The complex for simultaneous expression of the AtFT gene and the CRISPR / Cas9 system in plant cells to shorten the time to create gene-edited plants and the method for generating this complex.

Abstract

This invention is in the field of biotechnology, specifically gene editing in plants. Existing methods for creating genetically modified crops, especially for long-cycle species such as forestry trees, often require a long time due to growth and selection of mutant lines. The invention provides a complex for the simultaneous expression of the AtFT gene and the CRISPR / Cas9 system in plant cells. This complex includes a target vector carrying the AtFT gene and the hCas9 enzyme coding sequence, along with a vector to bind a specific gRNA sequence to the target gene. The two vectors are designed to recombine to form a complete construct allowing for the simultaneous expression of the aforementioned components. The complex according to the invention allows for the combination of early flowering induction with gene editing, thereby contributing to shortening the time required to create genetically modified crops.