PROCESS FOR EXTRACTING AND ENRICHING QUERCETIN COMPOUND FROM PERSIMMON PEEL

Abstract

The invention proposes a process for obtaining and enriching quercetin and its bioactive derivatives from persimmon peel using a deep eutectic solvent system combined with ethanol to improve extraction efficiency and increase selectivity for quercetin. The process includes the following steps: (i) washing persimmon peel to remove mechanical impurities; (ii) drying to a suitable moisture content; (iii) fine grinding and sieving to create a powder with uniform particle size; (iv) creating a deep eutectic solvent by mixing choline chloride with an organic acid with 2 to 6 carbon atoms; (v) mixing the deep eutectic solvent with ethanol to form a DES-ethanol solvent system; (vi) mixing this solvent system with persimmon peel powder at a solvent / material ratio of 20:1 to 40:1 (weight / volume), while mechanically stirring for 20-40 minutes at 30-60°C; (vii) perform ultrasonic extraction for 10–30 minutes at 50–60°C to enhance the release of the target compound; (viii) separate the extract by sedimentation, filtration, or centrifugation to remove solid residue;(ix) Purify the extract by silica gel column chromatography with a particle size of 40-63 µm, using an elution solvent system with increasing polarity consisting of ethyl acetate and ethanol to obtain the quercetin-rich fraction; (x) Concentrate the obtained fraction, preferably under reduced pressure to remove the solvent and enrich the quercetin and its derivatives; and (xi) Analyze the compound composition by HPLC-DAD. The procedure in this invention allows for improved recovery efficiency and quercetin enrichment compared to conventional organic solvent extraction methods, while also improving the purity of the obtained fraction.

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