The cultivation process for pearl oyster mushrooms (Agrocybe aegerita) uses a liquid-form, generation II spore culture.

Abstract

The invention relates to a process for cultivating the pearl mushroom Agrocybe aegerita using a liquid culture of secondary inoculum. Sawdust is moistened with lime water, incubated for 3 to 5 days, then mixed with corn bran, rice bran, and calcium carbonate. The substrate is packed in bags of 0.8 kg to 1.0 kg, sterilized at 118°C to 121°C, cooled below 28°C, and directly inoculated with 10 mL to 15 mL of liquid culture per bag. The bags are incubated at 23°C to 25°C, then fruiting bodies are formed at 20°C to 25°C, with a humidity of 80% to 95% and a CO₂ concentration below 1,000 ppm. The process shortens mycelial growth time, maintains a biological efficiency of approximately 20%, and is suitable for practical production.