The cultivation process for Trametes versicolor mushrooms uses a liquid-form, generation II spore culture.

Abstract

The invention relates to a process for cultivating Trametes versicolor mushrooms using a liquid culture of secondary spawn. Sawdust is moistened with lime water, incubated for 3 to 5 days, then mixed with corn bran, rice bran, and calcium carbonate. The substrate is packed in bags of 1.2 to 1.4 kg, sterilized at 118°C to 120°C, cooled below 28°C, and directly inoculated with 15 to 20 mL of liquid spawn per bag. The bags are incubated at 20°C to 24°C, then fruiting bodies are formed at 24°C to 28°C, with a humidity of 80% to 95% and a CO₂ concentration below 1,000 ppm. The process shortens mycelial growth time, reduces fruiting body formation time, and maintains stable yield.