Procedures for isolation and purification of flavonoid compounds from the stems and leaves of high-mountain tamarind (phyllanthus anthopotamicus hand.-mazz.)

Abstract

This useful solution describes a process for isolating and purifying flavonoid compounds from the stems and leaves of Phyllanthus anthopotamicus, a species belonging to the Phyllanthaceae family. The plant material, after drying and grinding, was extracted using MeOH solvent. The extract was then fractionated using solvents of varying polarity, including n-hexane and EtOAc. The EtOAc fraction was further separated by silica gel column chromatography with a solvent system of increasing polarity to obtain intermediate fractions. These fractions were further purified using chromatographic methods such as silica gel, Sephadex LH-20, and reversed-phase chromatography (RP-18) to obtain pure flavonoid compounds. The process, following a useful solution, allowed for the isolation of four flavonoid compounds, including (+)-catechin, quercetin 3-O-(6"-O-galloyl)-β-glucopyranoside, astragalin, and apigenin 7-O-β-glucopyranoside, with high purity, contributing to the provision of standard sources for chemical research and evaluation of the biological activity of natural compounds.