Procedure for isolation and purification of chrysin, an Anti-inflammatory compound, from the fruit of the wild tamarind (phyllanthus emblica l.)

Abstract

The useful solution refers to a process for isolating and purifying the anti-inflammatory compound chrysin from the fruit of the wild gooseberry (Phyllanthus emblica L.). The process according to the solution includes the following steps: a) material preparation; b) extraction of powdered wild gooseberry fruit; c) liquid-liquid fractional extraction to obtain the ethyl acetate fraction; d) collection of the fractional extract containing chrysin; e) collection of crude chrysin; and f) purification of the chrysin compound. The process followed to obtain the compound chrysin, which has anti-inflammatory effects based on inhibiting NO production.