Multi-epitope antigen, immunogenic composition comprising said antigen, pneumococcal diagnostic kit, and uses of said antigen

Abstract

The present invention relates to a multi-epitope antigen comprising 20 epitope blocks of 20 to 36 amino acids from proteins selected from the group consisting of NanA, PcsB, PhtD, Ply, PncO, StkP, PspA-F1 and PspA-F2. In addition, the present invention relates to an immunogenic composition comprising said antigen and a pharmaceutically acceptable vehicle and / or adjuvant. In addition, a pneumococcal diagnostic kit comprising said antigen or a biologically active fragment thereof bound to a detectable fraction; an antibody generated by said antigen; and instructions for use is also disclosed. In addition, the present invention relates to the use of said antigen to be used in the preparation of a vaccine to prevent diseases caused by pneumococcus bacteria; and for the production of monoclonal and polyclonal antibodies. Lastly, the present invention relates to an in vitro method for diagnosing pneumococcus which comprises applying the said antigen or a biologically active fragment of it bound to a detectable fraction, in contact with samples of body fluids selected from among blood, mucus expelled from the lower airways and urine.

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