Method of non-invasive fetal DNA and fetal fraction analysis using multiplex digital PCR reaction

WO2024263045A1PCT designated stage expired Publication Date: 2024-12-26INST CENT ZDROWIA MATKI POLKI
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Patent Information

Application Number
PCT/PL2024/050042
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-06-20
Filing Date
2024-06-20
Publication Date
2024-12-26

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Abstract

A method of cell-free fetal DNA (cffDNA) analysis for sex determination and fetal fraction content percentage evaluation, characterized in that biological material is collected from a pregnant female patient, cffDNA is isolated, the fetal DNA is evaluated, a multiplex digital PCR reaction is performed in order to amplify genes on Y chromosome, including RPS4Y2, SRY, USP9Y, and reference genes RPPH1, TERT, EIF2C1, and the data is analyzed.
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Citation Information

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