Method for evaluating intercellular interaction of neuroinflammation

A co-culture system of human-derived neural cells with inflammatory stimuli assesses cell interactions by monitoring markers and activity, addressing the limitations of existing methods and providing insights into neuroinflammation and therapeutic applications.

WO2025005230A1PCT designated stage expired Publication Date: 2025-01-02FUJIFILM CORP
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Patent Information

Application Number
PCT/JP2024/023480
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-04-26
Filing Date
2024-06-28
Publication Date
2025-01-02

AI Technical Summary

Technical Problem

Current methods for evaluating cell-cell interactions in neuroinflammation cannot determine which cells are activated and when they interact, limiting the understanding of their effects on normal brain function and pathological mechanisms.

Method used

A co-culture system containing human-derived astrocytes, neurons, microglia, and oligodendrocytes is used, with inflammatory stimuli applied to assess changes in inflammatory response markers, neural activity, and cell morphology over time to visualize and determine cell interactions.

Benefits of technology

This method allows for the evaluation of which cells interact with each other and at what time, providing insights into neuroinflammation mechanisms and potential therapeutic developments for neurodegenerative diseases.

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Abstract

The present invention addresses the problem of providing a method for evaluating intercellular interaction in neuroinflammation by using a co-culture containing human-derived neural cells capable of mimicking the function of a human brain. The present invention provides a method for evaluating intercellular interaction of neuroinflammation, the method comprising: a step for producing a co-culture containing at least two cells selected from the group consisting of human-derived astrocytes, human-derived neurons, human-derived microglia, and human-derived oligodendrocytes; a step for providing an inflammatory stimulus to a first cell contained in the co-culture; a step for detecting at least one selected from the group consisting of inflammatory reaction markers, cellular neuronal activity, and cell morphology in a cell contained in the co-culture; and a step for evaluating at least one change selected from the group consisting of the inflammatory reaction marker, the neuronal activity, and the cell morphology over time in the first cell and in a second cell included in the co-culture and different from the first cell.
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Citation Information

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