Viral vector packaging system and use thereof

By designing CAR binding molecules in the packaging system, the problem of false transduction of lentiviral vectors and retroviral vectors is solved, the detection accuracy and efficacy of CAR-T cell therapy are improved, and antigen escape is reduced.

WO2025209590A1 Publication Date: 2025-10-09SHENZHEN GENOCURY BIOTECH CO LTD
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Patent Information

Application Number
PCT/CN2025/087359
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-04-03
Filing Date
2025-04-03
Publication Date
2025-10-09

AI Technical Summary

Technical Problem

During the packaging of lentiviral or retroviral vectors, false transduction of CAR molecules between the viral envelope and the host cell membrane leads to inaccurate detection and antigen escape, affecting the efficacy of CAR-T cell therapy.

Method used

A packaging system is designed, comprising a polynucleotide encoding a chimeric antigen receptor and a CAR binding molecule. By assembling the CAR binding region in the packaging cell, the expression of CAR on the packaging cell membrane is reduced, false transduction is avoided, and the binding ability of the CAR molecule to the host cell is enhanced.

Benefits of technology

It improves the detection accuracy and efficacy of CAR-T cell therapy, reduces false transduction and off-target effects, and enhances the expression and recognition ability of CAR molecules in host cells.

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Abstract

Provided are a packaging system for packaging a lentiviral vector or a retroviral vector, and a use of the packaging system. Specifically, the provided packaging system comprises a polynucleotide encoding a CAR binding molecule, wherein the CAR binding molecule can bind to a CAR. Pseudotransduction is reduced when a host cell is transduced by the lentiviral vector or the retroviral vector packaged by the provided packaging system, and the ability to transduce a target cell expressing an antigen decreases, thereby significantly improving the targeting performance of the lentiviral vector or the retroviral vector in the transduction of immune cells such as T cells.
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