Paired index position-encoded sequencing

Abstract

Paired index position-encoded sequencing is described. A plurality of transposons may be generated, each of the plurality of transposons comprising two oligonucleotides coupled together in a 5'-to-5' orientation to encode a unique pair of index sequences that distinguishes one transposon of the plurality of transposons from other transposons of the plurality of transposons. The plurality of transposons may be integrated into genomic DNA during a sequencing workflow. The unique pair of index sequences of the plurality of transposons may be matched between sequencing reads obtained during the sequencing workflow. A genomic sequence of the biological sample may be reconstructed based on the matching.

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