Methods and compositions for selective control of signal generation efficiency
By forming ligation junctions with different efficiencies for first and second analytes, the method addresses signal crowding from highly expressed analytes, improving the detection and sequencing of other analytes in biological samples.
WO2026136945A1PCT designated stage Publication Date: 2026-06-2510X GENOMICS INC
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- 10X GENOMICS INC
- Filing Date
- 2025-12-19
- Publication Date
- 2026-06-25
AI Technical Summary
Technical Problem
Existing methods for in situ detection of analytes, such as nucleic acids, face challenges due to crowding of optical signals from highly expressed analytes, which overwhelm the ability to sequence other analytes of interest in a biological sample.
Method used
A method involving hybridization of first and second ligatable probes to form ligation junctions with different ligation efficiencies, followed by ligation and detection of signals from these junctions, allowing selective reduction of signals from highly expressed analytes.
Benefits of technology
This approach enables the selective control of signal generation efficiency, enhancing the ability to detect and sequence analytes of interest by reducing interference from highly expressed analytes.
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Smart Images
Figure US2025060754_25062026_PF_FP_ABST
Abstract
The present disclosure relates in some aspects to methods and compositions for selectively reducing the efficiency of signal generation from one or more analytes in a biological sample. In some aspects, the methods can improve sample analysis by reducing crowding of optical signals and / or reducing sequencing reads from highly expressed analytes, such as RNA transcripts.
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