Method of selecting homogenous CHO cells from a population of CHO cells
The use of DNA methylation bead-based arrays to select epigenetically homogenous CHO cells addresses the inconsistency in current CHO cell selection methods, improving the efficiency and consistency of protein production by ensuring genetic and phenotypic uniformity.
WO2026139355A1PCT designated stage Publication Date: 2026-07-02EVONIK OPERATIONS GMBH
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- EVONIK OPERATIONS GMBH
- Filing Date
- 2025-12-18
- Publication Date
- 2026-07-02
AI Technical Summary
Technical Problem
Current methods for selecting CHO cells for recombinant protein production are time-consuming, inaccurate, and fail to ensure genetic and phenotypic consistency, leading to inefficiencies and financial losses in bioproduction.
Method used
A method using DNA methylation bead-based arrays to compare methylation profiles of CHO cells, identifying epigenetically homogenous cells within a phenotypically homogenous population, ensuring stability and consistency in protein production.
Benefits of technology
Enhances the speed, quality, and efficiency of heterologous protein production by selecting and maintaining genetically and phenotypically identical CHO populations, reducing variability and financial losses.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention relates to a method of selecting epigenetically homogenous Chinese Hamster Ovary (CHO) cells from a population of phenotypically homogenous CHO cells, the method comprising the steps of: (a) determining a methylation profile from genomic material obtained from each of the phenotypically homogenous CHO cells in the population of cells, and (b) comparing the methylation profiles of (a) with one another wherein a significant similarity between the methylation profiles of at least a first and second CHO cell from the population of phenotypically homogeneous CHO cells is indicative of the first and second CHO cells being epigenetically homogenous; and wherein the methylation profiles in step (a) are measured using DNA methylation bead-based array.
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