Method for analyzing metabolite of intestinal bacterial flora and system for analyzing metabolite of intestinal bacterial flora

WO2026141307A1PCT designated stage Publication Date: 2026-07-02KOBE UNIV +1
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Patent Information

Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
KOBE UNIV
Filing Date
2025-12-22
Publication Date
2026-07-02

AI Technical Summary

Technical Problem

Conventional methods for analyzing metabolites of the gut microbiota are insufficient in comprehensiveness and quantity, often limiting the number of metabolites that can be quantitatively analyzed to a few dozen types, which results in incomplete data.

Method used

A method and system that involves an extraction process, a derivatization process, and a quantitative analysis process using chromatographic mass spectrometry with multiple columns and derivatization, allowing for the comprehensive and rapid quantitation of at least 100 types of metabolites.

Benefits of technology

The method significantly increases the number of metabolites that can be analyzed, enhancing the comprehensiveness of metabolite analysis by using a combination of non-derivative and derivative extracts through chromatographic mass spectrometry with different separation characteristics.

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Abstract

In an extraction treatment process, an extract containing a metabolite of intestinal bacterial flora is extracted from a culture solution 101. In a derivatization treatment process, derivatization treatment is performed on some of the extract to prepare a derivatized extract. In a quantitative analysis process, there are performed: a first quantitative analysis in which a non-derivatized extract that has not been derivatized is introduced into a first column 31 and subjected to quantitative analysis; a second quantitative analysis in which a non-derivatized extract is introduced into a second column 32 having separation characteristics different from those of the first column 31 and subjected to quantitative analysis; and a third quantitative analysis in which the derivatized extract is introduced into the second column 32 and subjected to quantitative analysis. By performing three quantitative analyses having different characteristics, it becomes possible to comprehensively perform quantitative analyses on a wider variety of metabolites.
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