Apparatus for generating liquid sample for airborne viruses

Abstract

The present invention may provide an apparatus for generating a liquid sample by generating an aerosol sample from air and then eluting the aerosol sample, in order to provide a virus detection sensor with a liquid sample for airborne viruses. The present invention may provide an apparatus capable of efficiently separating airborne particles of various sizes using a cyclone device and, in particular, effectively collecting fine virus particles. The present invention may provide an apparatus that integrates a process of combining airborne viruses collected from the air with an eluent to convert same into a liquid sample, and utilizes a cyclone not only for simple air-particle separation but also in the elution step. The present invention may provide an apparatus that enhances detection sensitivity by detecting sample concentration in real time through a particle sensor and adjusting the ratio of eluent to deionized water to provide a sample with optimal concentration to the virus detection sensor.

Description

Liquid sample generation device for airborne viruses

[0001] The present invention relates to an apparatus for generating a liquid sample for airborne viruses. Specifically, the present invention relates to an apparatus for generating a liquid sample by eluting an aerosol sample from air, in order to provide a liquid sample for airborne viruses to a virus detection sensor.

[0002]

[0003] Airborne viruses are one of the major causes of disease transmitted through the respiratory system. Particularly in enclosed spaces, airborne viruses can spread rapidly, significantly increasing the importance of technologies for early detection. As conventional surface sampling methods have limitations, the need for technologies to capture and analyze airborne viruses is emerging.

[0004] Currently, the representative technologies for capturing airborne particles are filtering and cyclone methods. The filtering method physically separates various particles based on size by filtering them through filter paper. On the other hand, the cyclone method utilizes centrifugal force to separate heavy particles from the airflow. However, conventional cyclone technology is primarily used to separate relatively large particles, so it has limitations in effectively capturing fine particles such as viruses.

[0005] Viruses exist in the air as nanometer-sized particles. While conventional filtering and cyclone methods are suitable for handling relatively large particles, capturing very small viral particles requires more precise capture technology. Since the concentration of viruses in the air is low, it is difficult to obtain a sufficient amount of sample after capture; therefore, combining this with concentration technology is essential for detection.

[0006] The novel method presented in this invention extends beyond merely using cyclone technology for capturing airborne particles to also utilizing it for eluting the captured viruses. The method of capturing airborne viruses using a cyclone device and combining them with an eluent to convert them into a liquid sample is a novel approach that is differentiated from existing technologies. In particular, in this invention, the cyclone plays the role of generating a liquid sample by simultaneously capturing viruses and suspending them in an eluent.

[0007] The standard method for detecting viruses involves suspending captured viruses in an eluent to convert them into a liquid state, and then supplying this to a biosensor or analysis device. Generally, an eluent such as PBS (Phosphate Buffered Saline) is used to ensure that the captured viruses are stably suspended in the liquid. The present invention combines cyclone capture technology with this method to provide a technology that effectively converts airborne viruses into a liquid sample.

[0008] A sensor for virus detection confirms the presence of viruses by detecting liquid samples containing captured viruses in real time. Since the sensor requires very high sensitivity, it is important to optimize the concentration of the captured sample before delivery. To this end, a technology is required to elute the captured viruses and accurately deliver a liquid sample of an appropriate concentration to the virus detection sensor. This invention addresses this through the control of the eluent concentration and an optimal sample delivery method.

[0009] The present invention provides an integrated system for converting airborne viruses into liquid samples by combining airborne virus capture technology using a cyclone with an elution device. The process involves organically separating particles of various sizes from the air using a cyclone, suspending the captured viruses in an eluent, and finally delivering them to a virus detection sensor. In this process, fluid control technology is also applied to regulate the concentration of the eluent and optimize the sample concentration.

[0010] This invention presents a new technology that improves upon existing cyclone technology to effectively capture even minute virus particles in the air and elute them to convert them into liquid samples. In particular, a key innovation of this invention is the method of utilizing the cyclone in the elution process, rather than limiting it to simple separation of airborne particles. Through this, the primary objective of this invention is to enhance the detection sensitivity of airborne viruses and implement a system capable of detecting viruses in real time.

[0011]

[0012] The present invention aims to solve the following problems in order to solve the aforementioned problems.

[0013] The present invention aims to provide an apparatus for generating a liquid sample by eluting an aerosol sample after generating an aerosol sample from air, in order to provide a liquid sample for airborne viruses to a virus detection sensor.

[0014] The present invention aims to provide a device capable of efficiently separating particles of various sizes in the air using a cyclone device, and in particular, effectively capturing fine virus particles.

[0015] The present invention is a technology that integrates a process of combining airborne viruses with an eluent to convert them into a liquid sample, and aims to provide a device that utilizes a cyclone not only for simple separation of airborne particles but also in the elution stage.

[0016] The present invention aims to provide a device that increases detection sensitivity by detecting the concentration of a sample in real time through a particle sensor and providing a sample of an appropriate concentration to a virus detection sensor by adjusting the ratio of the eluent to the deionized water.

[0017] The present invention aims to provide a device that combines an elution device, a fluid control system, and a particle sensor to convert airborne viruses into a liquid sample and optimize the sample concentration to maximize detection efficiency.

[0018] The present invention aims to provide an apparatus that prevents contamination during a continuous process and provides accurate and reliable samples, comprising an automated system capable of washing and disinfecting the collection surface with deionized water and ethanol after sample generation.

[0019] The problems solved by the present invention are not limited to those mentioned above, and other problems not mentioned will be clearly understood by a person skilled in the art from the description below.

[0020]

[0021] According to various embodiments of the present invention, a liquid sample generating device for airborne viruses comprises: a cyclone device for generating an aerosol sample by removing particles from air; an elution device for generating a liquid sample from the aerosol sample; and a control device connected to the cyclone device and the elution device, wherein the cyclone device comprises: one or more cyclones for filtering the airborne particles; It includes one or more particle sensors for detecting particle characteristics of particles introduced into the cyclone device and particles filtered by the one or more cyclones; wherein the one or more cyclones are connected in series and each of the one or more cyclones is configured to filter particles of different particle sizes, and the one or more particle sensors include an inlet particle sensor at the inlet where air is introduced into the cyclone device and cyclone particle sensors corresponding to each of the one or more cyclones; and the elution device comprises: a collection surface which is a space where the aerosol sample is collected and an eluent, which is phosphate buffered saline (PBS), is injected; an eluent injection device for injecting the eluent into the collection surface; a deionized water injection device for injecting deionized water (DI water) to control the concentration of the eluent injected into the collection surface by diluting the eluent into the collection surface; and an ethanol injection device for injecting ethanol (EtOH) into the collection surface for disinfecting the collection surface. A filtering device for filtering particles larger than a predetermined size from the aerosol sample in the eluent or a mixture of the eluent and the deionized water; a sample collection unit for collecting the liquid sample that has passed through the filtering device in the mixture;A liquid sample generating device is provided, comprising: a waste collection unit for collecting waste filtered by the filtering device among the above mixture; and a fluid control device for controlling the flow of the eluent, the deionized water, the ethanol, the mixture, and the liquid sample; wherein the control device comprises: a transceiver; a memory; an input device; an output device; and a processor functionally connected to the cyclone device, the eluent device, the transceiver, the memory, the input device, and the output device.

[0022]

[0023] The present invention may provide an apparatus for generating a liquid sample by eluting an aerosol sample from air, in order to provide a liquid sample for airborne viruses to a virus detection sensor.

[0024] The present invention can provide a device capable of efficiently separating particles of various sizes in the air using a cyclone device, and in particular, effectively capturing fine virus particles.

[0025] The present invention provides a technology that integrates a process of combining airborne viruses with an eluent to convert them into a liquid sample, and can provide a device that utilizes a cyclone not only for simple separation of airborne particles but also in the elution stage.

[0026] The present invention can provide a device that increases detection sensitivity by detecting the concentration of a sample in real time through a particle sensor and adjusting the ratio of the eluent to the deionized water to provide a sample of an appropriate concentration to a virus detection sensor.

[0027] The present invention can provide a device that combines an elution device, a fluid control system, and a particle sensor to convert airborne viruses into a liquid sample and optimize the sample concentration to maximize detection efficiency.

[0028] The present invention can provide an apparatus that prevents contamination during a continuous process and provides accurate and reliable samples by including an automated system capable of washing and disinfecting the collection surface with deionized water and ethanol after sample generation.

[0029] The effects of the present invention are not limited to those mentioned above, and other unmentioned effects will be clearly understood by those skilled in the art from the description below.

[0030]

[0031] FIG. 1 illustrates an example of a detection system for airborne viruses according to an embodiment of the present invention.

[0032] FIG. 2 illustrates an example of the configuration of a liquid sample generating device for airborne viruses according to an embodiment of the present invention.

[0033] FIG. 3 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.

[0034] FIG. 4 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.

[0035] FIG. 5 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.

[0036] FIG. 6 illustrates an example of an elution device in an airborne virus detection device implemented according to an embodiment of the present invention.

[0037]

[0038] Hereinafter, embodiments of the present invention are described in detail with reference to the attached drawings so that those skilled in the art can easily implement the present invention. The present invention may be embodied in various different forms and is not limited to the embodiments described herein.

[0039] FIG. 1 illustrates an example of a detection system for airborne viruses according to an embodiment of the present invention.

[0040] Referring to FIG. 1, a detection system for airborne viruses according to an embodiment of the present invention consists of a cyclone, elution, and virus detection.

[0041] A detection system for airborne viruses according to an embodiment of the present invention includes a cyclone device, an elution device, a virus detection sensor, and an air discharge device. A detailed description of each component is as follows:

[0042] 1. Cyclone Device

[0043] A cyclone device serves to separate and capture particles of various sizes from the air. In the present invention, the cyclone device comprises one or more cyclones. When the one or more cyclones are multiple cyclones, the multiple cyclones are connected in series, and the multiple cyclones are composed of several cyclones connected in series, and each cyclone effectively separates particles of different sizes.

[0044] (1) Particle separation principle: The cyclone device rotates the air using centrifugal force, separating heavy particles (large particles) from the airflow and transferring light particles to the next cyclone. Through this process, even small particles such as viruses can be separated and captured step by step.

[0045] (2) Particle Sensor: A particle sensor corresponding to each cyclone is installed to detect the size and concentration of particles in the air in real time. This allows for the identification of the characteristics of the captured particles and subsequent adjustment of the amount and concentration of the eluent during the elution stage.

[0046] 2. Elution Device

[0047] The elution device is a mechanism that suspends virus particles captured by a cyclone in an eluent, converting them into a liquid sample. At this stage, the viruses captured from the air are converted into a liquid state and prepared for delivery to a virus detection sensor.

[0048] (1) Collection Surface: Captured virus particles gather on the collection surface, and an eluent such as PBS (Phosphate Buffered Saline) is injected into this surface so that the viruses are suspended in the eluent.

[0049] (2) Injection of eluent: Virus particles captured from the air are eluted into an analyzable liquid. During this process, deionized water (DI water) is mixed into the eluent to control the concentration of the eluent, and the amount of eluent injected is automatically adjusted according to the particle concentration.

[0050] (3) Filtering device: Filters the eluted mixture to remove particles larger than a certain size. It removes unwanted particles from the liquid sample through a membrane filter or a rotating liquid impactor, and produces a high-purity liquid sample.

[0051] (4) Lysis buffer injection device: Includes a buffer injection device for mixing lysis buffer into the liquid sample generated through the filtering process. This device automatically mixes the filtered liquid sample with the lysis buffer to efficiently extract viral proteins and nucleic acids (RNA / DNA) from the sample. Through this process, the sample completes chemical treatment to increase analytical sensitivity before being delivered to the virus detection sensor.

[0052] (5) Cleaning and disinfection system: After the elution and filtering process is finished, the inside of the elution device is cleaned and disinfected using ethanol (EtOH) and deionized water, which prevents sample contamination and maintains reliable analysis.

[0053] 3. Virus Detection Sensor

[0054] The virus detection sensor plays the role of detecting the presence of viruses by analyzing liquid samples generated from the elution device. The sensor is a highly sensitive device capable of detecting even small amounts of virus particles in real time.

[0055] (1) Liquid sample provision: The liquid sample obtained from the elution device is transferred to the virus detection sensor, and a sample of an appropriate concentration is provided to optimize detection sensitivity. To this end, the amount of the sample can be adjusted or a dilution process can be performed so that the sample particle concentration matches the reference concentration.

[0056] (2) Real-time detection: The virus detection sensor analyzes the virus concentration of the provided sample in real time and immediately determines the presence of the virus. This detection sensor is based on antibody or gene detection technology for specific viruses and can detect various viruses.

[0057] According to one embodiment of the present invention, a virus detection sensor may comprise: a reaction membrane for causing a chemical or biological reaction with a virus in a liquid sample; a water-permeable polymer layer of polyvinyl alcohol (PVA) provided on the reaction membrane; and a signal amplification pad located on the water-permeable polymer layer. A signal amplification buffer is injected into the reaction membrane simultaneously or sequentially with the ammonium sample, and the water-permeable polymer layer may delay the release of a signal amplification material contained in the signal amplification buffer.

[0058] According to one embodiment of the present invention, a virus detection sensor comprises: a sample pad; an absorbent pad; a membrane pad disposed between the sample pad and the absorbent pad; a conjugate pad disposed between the sample pad and the membrane pad; and a TMB (Tetramethylbenzidine) pad disposed between the membrane pad and the conjugate pad. The apparatus comprises a TMB reinforced pad disposed on the sample pad, wherein the test line of the membrane pad comprises an antibody that specifically binds to any one of COVID-19 (CoV), AdV (Adenovirus), Influenza A, and Influenza B, the conjugate pad comprises platinum nanoparticles (Pt NP), the TMB reinforced pad is shorter in length than the sample pad, the TMB reinforced pad is disposed on one side of the sample pad, the TMB reinforced pad comprises any one of citric acid, malic acid, maleic acid, and fumaric acid as an acid substance, the concentration of said acid substance is 0.01 to 0.5 M, the TMB reinforced pad comprises Na2O2, and the concentration of said Na2O2 may be 10 to 100 mM.

[0059] According to one embodiment of the present invention, a virus detection sensor comprises: a support of a single length member; a single sample pad disposed at one end on the support; a single absorbent pad disposed at the other end on the support; a first strip pad disposed on the support and disposed between the sample pad and the absorbent pad; a second strip pad disposed on the support and disposed between the sample pad and the absorbent pad and spaced apart from the first strip pad; and a first conjugate pad disposed between the sample pad and the first strip pad. The apparatus may include a second conjugate pad disposed between the sample pad and the second strip pad, wherein the first strip pad comprises at least two first test lines, and the at least two first test lines comprise at least two antibodies that specifically bind to any one of COVID-19 (CoV), RSV, Influenza A, and Influenza B, the second strip pad comprises at least two second test lines, and the second test lines comprise at least two antibodies that specifically bind to any one of COVID-19 (CoV), RSV, Influenza A, and Influenza B, the first conjugate pad comprises at least two metal nanoparticles bound to at least different detection antibodies, and the second conjugate pad may comprise at least two metal nanoparticles bound to at least different detection antibodies.

[0060]

[0061] 4. Air Exhaust Device

[0062] The air exhaust device serves to purify the air used within the virus detection device and safely discharge it to the outside. It is designed to prevent potential contamination risks that may occur during the virus capture and elution processes and to maintain the safety of the experimental environment.

[0063] (1) Air purification filter device: Purifies air that has been introduced through a cyclone device and passed through an elution device. It removes residual particles, viruses, or other harmful substances from the air to produce clean air.

[0064] (1-1) Configuration: It effectively removes airborne fine particles and viruses by including a High-Efficiency Particle Capture (HEPA) filter or an activated carbon filter. Purification efficiency can be maximized with a multi-stage filtering system (e.g., a pre-filter + HEPA filter). Additionally, it may include a plasma generator and perform sterilization using plasma light.

[0065] (1-2) Operating principle: As air passes through the purification filter, harmful substances are filtered out, and clean air moves to the exhaust line.

[0066] (2) Air outlet: Safely discharges purified air into the external environment. Prevents additional contamination during the process of the discharged air leaking out.

[0067] (2-1) Configuration: The air outlet is designed to maintain smooth airflow and includes a backflow prevention device. Sound-absorbing structures are added around the outlet to reduce noise, thereby minimizing noise generated during operation.

[0068] (3) System Integration: The air exhaust device operates in conjunction with other components within the virus detection device. Air generated from the cyclone device passes through the elution device and is purified through the air purification filter device before being discharged. The flow rate, filter status, and discharge efficiency of the exhaust air can be monitored in real time through the integrated control system.

[0069] (4) Summary of operating principles

[0070] (4-1) Air Purification: Air entering the air exhaust device has viruses and harmful substances removed through a purification filter.

[0071] (4-2) Air Discharge: Purified air is discharged to the outside through the outlet, and backflow prevention and noise reduction are ensured during this process.

[0072] (4-3) Safety Management: The condition of the filter can be monitored, and the replacement or cleaning process can be automated to maintain continuous purification performance.

[0073] The operation process of the airborne virus detection system according to an embodiment of the present invention is as follows.

[0074] (1) Air intake and particle collection: Airborne particles are introduced into the cyclone device and are separated and collected according to size as they pass through each cyclone. Particles of a specific size are collected in a form suitable for sensor analysis.

[0075] (2) Elution and Filtering: The captured viruses gather on the collection surface and are suspended in the eluent injected therein, converting them into a liquid sample. Subsequently, unnecessary particles are filtered out through a filtering process, producing a high-purity sample.

[0076] (3) Lysis Buffer Mixing: The liquid sample generated through the filtering process is mixed with lysis buffer. This mixing process extracts viral proteins and nucleic acids (RNA / DNA) from the sample to maximize detection sensitivity.

[0077] (4) Virus detection: The generated liquid sample is transferred to a virus detection sensor, and the presence of viruses is analyzed in real time.

[0078] (5) Air Purification and Discharge: The air used for virus detection is purified through an air purification filter device. The purified air is safely discharged to the outside through an air outlet, ensuring that contamination and backflow are prevented during this process.

[0079] The detection system for airborne viruses according to an embodiment of the present invention provides an integrated detection solution capable of capturing and analyzing viruses present in the air in real time, and is designed to effectively detect and respond to the spread of viruses in various environments.

[0080] FIG. 2 illustrates an example of the configuration of a liquid sample generating device for airborne viruses according to an embodiment of the present invention.

[0081] Referring to FIG. 2, a detection system for airborne viruses according to an embodiment of the present invention includes a liquid sample generating device (1000) and a virus detection sensor (2000).

[0082] A liquid sample generating device (1000) for airborne viruses according to an embodiment of the present invention includes a cyclone device (1100), an elution device (1200), a control device (1300), and an air discharge device (1400).

[0083] Air from outside the liquid sample generating device (1000) flows into the cyclone device (1100). The cyclone device (1100) provides an aerosol sample of airborne viruses to the elution device (1200). The elution device (1200) provides a liquid sample of airborne viruses to the virus detection sensor (2000).

[0084] The cyclone device (1100) includes a cyclone (1101) for large particles, a cyclone (1102) for medium-sized particles, a cyclone (1103) for small particles, a particle sensor (1104) for all particles entering the cyclone device (1100), a particle sensor (1105) for particles captured by the cyclone (1101) for large particles, a particle sensor (1106) for particles captured by the cyclone (1102) for medium-sized particles, and a particle sensor (1107) for particles captured by the cyclone (1103) for small particles. The cyclone device (1100) is a key device for separating and capturing particles of various sizes in the air, thereby enabling efficient separation of airborne particles during the virus detection process. In FIG. 2, three cyclones (1101, 1102, 1103) are shown, but this is exemplary and it is obvious that one or more various numbers of cyclones may be applied. Additionally, it is obvious that one or more various numbers of particle sensors (1105, 1106, 1107) for the cyclones (1101, 1102, 1103) may also be applied. A detailed description of each component of the cyclone device (1100) is as follows.

[0085] The cyclone for large particles (1101) serves to separate and capture large, heavy particles contained in the air. The cyclone for large particles (1101) purifies the airflow by removing relatively large particles (e.g., dust, pollen, etc.) from the air. Large, heavy particles are separated by being pushed against the walls by centrifugal force. The large particle cyclone (1101) is primarily designed to capture particles larger than 10 micrometers (μm). This is the first step in purifying the airflow so that the remaining cyclone can handle smaller particles.

[0086] The cyclone (1102) for medium-sized particles processes medium-sized particles that have passed through the first cyclone. The cyclone (1102) for medium-sized particles captures relatively small particles (e.g., bacteria, fine dust, etc.) and prepares to capture small particles more effectively. It is an intermediate step for filtering medium-sized particles that were not removed in the first cyclone. The medium-sized particle cyclone (1102) is designed to capture particles between 2 micrometers (μm) and 10 μm.

[0087] The cyclone for small particles (1103) serves to capture the smallest particles in the air. The cyclone for small particles (1103) captures nanometer-sized particles (e.g., virus particles). It is the final step in detecting and separating fine particles, such as viruses, in the air. The small particle cyclone (1103) is primarily designed to capture particles smaller than 1 micrometer (μm). This plays an important role in separating small particles from the air.

[0088] The particle sensor (1104) for all particles detects all particles in the air entering the cyclone device (1100). The particle sensor (1104) for all particles detects and records the characteristics (size, concentration, mass, etc.) of the particles in the air entering the cyclone device in real time. This allows for the identification of the overall distribution of particles entering the device. The particle sensor (1104) for all particles analyzes the size distribution and concentration of particles in the air in real time, providing important data for system control and adjustment of the amount of eluent injected.

[0089] The particle sensor (1105) for large particles is a sensor that detects particles captured by the large particle cyclone (1101). The particle sensor (1105) for large particles detects the number, size, and concentration of particles separated from the cyclone (1101) for large particles to determine whether the particles have been properly captured. It provides data so that the system can adjust the appropriate amount of eluent according to the characteristics of the captured particles.

[0090] The particle sensor (1106) for medium-sized particles is a sensor that detects particles captured by the medium-sized particle cyclone (1102). The particle sensor (1106) for medium-sized particles detects and records the number, size, and concentration of particles captured for medium-sized particles in real time. This allows for verification of the capture efficiency of medium-sized particles and enables adjustment of the eluent injection and filtering processes.

[0091] The particle sensor (1107) for small particles is a sensor that detects fine particles captured by a small particle cyclone (1103). The particle sensor (1107) for small particles detects small particles in nanometer units and monitors the size and concentration of the captured particles in real time. It is primarily used to determine whether fine particles, such as viruses floating in the air, have been captured.

[0092] When air is introduced into the cyclone device (1100), the particles are gradually separated as they pass through cyclones (1101, 1102, 1103) for large particles, medium-sized particles, and small particles. Particle sensors (1105, 1106, 1107) corresponding to each cyclone monitor the characteristics of the captured particles in real time and provide data to the system. Through this data, the amount of eluent injected and filtering efficiency can be controlled. This configuration plays an important role in effectively capturing particles of various sizes from the air to generate accurate and reliable samples during the subsequent virus detection process. The cyclone device (1100) is optimized to efficiently separate particles of various sizes present in the air to generate a liquid sample for final delivery to a virus detection sensor.

[0093] A particle sensor is a device that detects fine particles contained in air or liquid and measures their size, number, concentration, and mass. Particle sensors play an important role in various fields, including environmental monitoring, industrial process control, medical diagnosis, and virus detection. Generally, they detect particles using optical, electrical, or mechanical principles.

[0094] The configuration of the particle sensor is as follows.

[0095] (1) Light source: Serves to emit light along the path through which particles pass. Generally, a laser or LED light source is used to shine light onto the particles and initiate the detection process. The light emitted from the light source interacts with the particles and is scattered, absorbed, or reflected.

[0096] (2) Detector: Detects scattered light generated after particles interact with light, and calculates the size and number of particles. Generally, photodiodes, CCDs, or CMOS sensors are used to detect changes in light and convert them into electrical signals.

[0097] (3) Flow Path (or Measurement Chamber): This is the path through which particles flow, and it is the section within the sensor where particles interact with light. Particles are measured as they pass between the light source and the detector through this flow path. The flow path is designed to ensure that particles flow at a constant rate and provides an environment where particles can sufficiently interact with optical signals.

[0098] (4) Electronic circuit section: A device that processes and analyzes detected data, amplifying or filtering electrical signals generated by the sensor and converting them into digital signals. It processes the detected signals to produce meaningful data such as particle size, number, and concentration.

[0099] (5) Processor and Memory: These functions to process, store, or transmit data collected from sensors. The processor analyzes the data in real time, and the memory can store the data for a certain period. The processor analyzes the characteristics of the particles through the collected data and generates appropriate control signals based on this analysis.

[0100] The operating principle of a particle sensor is as follows. Particle sensors are generally based on optical principles and operate through the following procedure.

[0101] (1) Emission of light and passage of particles: A light source emits light, and particles pass through a path between the light source and the detector, interacting with the light. This interaction occurs in the form of light being scattered, reflected, or absorbed by the particles.

[0102] (2) Scattering and detection of light: When particles scatter or absorb light, the resulting change in light is detected by a detector. Larger particles scatter more light, while smaller particles scatter less. The detector converts these changes into electrical signals.

[0103] (3) Analysis of particle size and number: The detector analyzes the size of particles by measuring the intensity of scattered light. Large particles generate strong signals, and small particles generate weak signals, and based on this, the size and number of particles can be calculated.

[0104] (4) Calculation of concentration and mass: Concentration can be measured by calculating how many particles there are per unit time or per unit volume. Additionally, since the mass of a particle is proportional to its size, the mass of the particle can also be inferred.

[0105] (5) Real-time data processing: The electronic circuit and processor process data in real time to calculate the size, number, concentration, mass, etc. of the particles, and transmit or store the results in the system.

[0106] The main functions of the particle sensor are as follows.

[0107] (1) Particle size detection: The size of particles can be measured by the degree of light scattering or absorption. Since the way light scatters varies depending on the size, particle sizes are distinguished through this.

[0108] (2) Particle count measurement: The number of particles can be measured by analyzing the frequency or intensity of light scattered as particles pass through the detection zone. This is calculated by recording the frequency or intensity of light scattered by each particle.

[0109] (3) Concentration measurement: The concentration of particles can be calculated based on the number of particles detected per unit volume or time. This allows for determining the particle density in air or liquid.

[0110] (4) Mass measurement: The total mass of the particles can be calculated by combining particle size and concentration. Since size and mass are proportional, the larger the size, the greater the mass.

[0111] (5) Real-time monitoring: Particle sensors detect particle characteristics and process data in real time, enabling immediate response and monitoring. This plays an important role in air quality monitoring and virus detection.

[0112] The operation process of the particle sensor is as follows.

[0113] (1) Air or liquid inflow: Air or liquid containing particles moves through the sensor's flow path.

[0114] (2) Interaction with light source: Particles interact with light from a light source, scattering or absorbing the light. Changes in light occurring during this process are detected by a detector.

[0115] (3) Signal conversion: The detected change in light is converted into an electronic signal, which is used to calculate particle size, number, concentration, etc.

[0116] (4) Data processing: The processor processes data in real time and stores or transmits the results externally. This allows for the analysis of particle characteristics.

[0117] The application fields of particle sensors are as follows.

[0118] (1) Air quality monitoring: Particle sensors are widely used to monitor the concentration of fine dust (PM2.5, PM10) or harmful particles in indoor and outdoor air.

[0119] (2) Virus detection: The presence of viruses in the air or liquid can be determined by measuring the size and concentration of virus particles in real time.

[0120] (3) Industrial process control: Used to improve quality control by monitoring the concentration of impurities or fine particles in the manufacturing process.

[0121] (4) Medical and pharmaceutical fields: Used to analyze particles in drugs during pharmaceutical processes or to detect particles in medical equipment.

[0122] Particle sensors are devices that utilize optical principles to detect and analyze fine particles in air or liquids. They can measure particle size, number, concentration, and mass in real time and play an essential role in various industrial fields.

[0123] The elution device (1200) includes a collection surface (1201), an eluent injection device (1202), a deionized water injection device (1203), an ethanol injection device (1204), a filtering device (1205), a sample collection unit (1206), a waste collection unit (1207), a fluid control device (1208), and a buffer injection device (1209). The elution device (1200) serves to suspend viruses captured from the air in an eluent to convert them into a liquid sample, filter the sample, and then separate the sample from the waste to provide a sample for virus detection. A detailed description of each component of the elution device (1200) is as follows.

[0124] The collection surface (1201) is a space where virus particles captured by the cyclone device (1100) are collected, and is a surface into which an eluent is injected to suspend the virus particles in a liquid state. Particles (especially viruses) captured from the air are collected on the collection surface (1201), and an eluent (PBS) is injected to suspend the viruses in a liquid state. This surface is optimized to allow particles to come into contact with the eluent, thereby maximizing suspension efficiency. The material of the collection surface does not react with the eluent and is designed to effectively collect virus particles. It is mainly treated with a smooth surface so that virus particles can be easily washed away by the eluent.

[0125] The material and shape of the collection surface (1201) are important factors determining the performance of the elution device, playing a key role in efficiently capturing fine particles such as viruses and maximizing contact with the eluent to properly convert the sample. The material of the collection surface (1201) must be a smooth, inert material. The collection surface (1201) is designed with a smooth material so that virus particles do not easily adhere to it and are easily washed away by the eluent. This material must be an inert substance to prevent unnecessary chemical reactions between the particles and the eluent. Materials commonly used for the collection surface (1201) include glass, polytetrafluoroethylene (PTFE), and stainless steel. Glass is frequently used for collection surfaces because it is inert and has chemically stable properties. Additionally, the smooth surface of glass helps virus particles to be easily removed by the eluent. PTFE has inert properties and high chemical resistance, which minimizes reactions with the eluent and prevents sample contamination. Furthermore, the smooth surface of PTFE prevents viruses from easily adhering. Stainless steel is highly durable and chemically stable, making it suitable for systems requiring high durability. However, the surface of stainless steel must be finished smoothly.

[0126] The collection surface (1201) must be able to withstand repeated elution processes, washing, and disinfection. Since it is repeatedly washed and disinfected with deionized water or ethanol, it requires durability that prevents wear even with prolonged use. The collection surface (1201) must be easily washed and disinfected with ethanol and deionized water, and must be easy to maintain so that no residue remains.

[0127] The shape of the collection surface (1201) may be a flat collection surface, or a collection surface with an incline or curve, or a collection surface with fine grooves.

[0128] The flat collection surface (1201) allows virus particles to be evenly distributed and enables the eluent to flow easily. The flat collection surface is advantageous for the eluent to evenly cover the surface and effectively suspend virus particles. The flat collection surface has a simple structure, allowing the eluent to spread uniformly and come into contact with virus particles as it flows. The flat collection surface is easy to clean and disinfect because any remaining particles can be easily washed away after elution. The flat collection surface is a surface shape frequently used in laboratories and has a structure that makes it easy to control the flow of the eluent.

[0129] A collection surface (1201) with a slope or curve helps the eluent flow down naturally by gravity. The collection surface with a slope or curve makes the eluent more efficient by ensuring better distribution of the eluent and preventing particles from remaining on the surface. Since the eluent flows down naturally without pooling on the collection surface, particles can come into more efficient contact with the eluent and be suspended. Even during washing, the eluent is easily discharged without remaining.

[0130] The collection surface (1201) with micro-grooves is designed with a shape that allows the eluent to flow while providing a position where particles can be fixed to the surface by adding micro-grooves or patterns to the surface. This shape prevents particles from flowing down and increases the contact time with the eluent, thereby enabling efficient suspension. The collection surface with micro-grooves increases contact with the eluent while the micro-grooves temporarily hold the particles. This helps more particles to be suspended in the eluent and contributes to increasing elution efficiency.

[0131] The collection surface (1201) must be designed so that the eluent spreads evenly over the collection surface to maximize contact with particles. Therefore, the smoothness, slope, and shape of the grooves of the surface are important factors in controlling the flow of the eluent. The collection surface (1201) must be designed with a structure that allows sufficient contact between virus particles and the eluent, and for this purpose, the shape and texture of the surface are important. The collection surface must be designed so that no residue remains after elution and that cleaning and disinfection are easy. This is an important factor in increasing the reusability of the system and preventing contamination.

[0132] The collection surface (1201) is made of a smooth, inert material to maximize virus capture and interaction with the eluent, and is designed with a flat, sloped, or micro-grooved structure to allow the eluent to flow effectively. Through this design, virus particles in the air are efficiently eluted, and accurate virus detection becomes possible.

[0133] The eluent injection device (1202) is a device that suspends viruses by injecting an eluent into a collection surface (1201). The eluent injection device (1202) converts captured virus particles into a liquid state by injecting an eluent (PBS) into the collection surface. The amount of eluent injected is controlled according to the quantity and characteristics of the collected particles. In the eluent injection device (1202), the amount and injection speed of the eluent are controlled based on information obtained from the particle sensor, and the sample concentration is optimized by precisely managing the required volume of eluent.

[0134] The deionized water (DI Water) injection device (1203) is a device that injects deionized water into the collection surface to control the concentration of the eluent or for cleaning. The deionized water (DI Water) injection device (1203) injects deionized water mixed with the eluent to control the concentration of the collected particle sample. It also serves to clean the collection surface after elution to remove residue. In the deionized water (DI Water) injection device (1203), deionized water is used to dilute the concentration of the eluent and to clean the collection surface in preparation for the next sampling. The cleaning step prevents sample contamination and increases the reliability of the system.

[0135] The ethanol (EtOH) injection device (1204) is a device that injects ethanol to disinfect the system after the elution process. The ethanol (EtOH) injection device (1204) disinfects the system after the elution process is completed to ensure that no viruses or other contaminants remain. This is an essential process to prevent cross-contamination during reuse. In the ethanol (EtOH) injection device (1204), ethanol is a powerful disinfectant that completely disinfects the system after elution to remove virus particles or impurities. The disinfection process plays an important role in increasing the reliability of the sample.

[0136] The filtering device (1205) serves to filter out particles larger than a certain size from the eluted liquid sample. The filtering device (1205) purifies the sample using a membrane filter or a rotary liquid impactor. The filtering device (1205) filters out large particles (e.g., particles larger than 1 μm) from the eluted mixture to obtain a pure virus sample. This ensures that only small particles, such as viruses, are delivered to the detection sensor. The filtering device (1205) uses one or both of a membrane filter and a rotary liquid impactor. The membrane filter allows only small particles to pass through, while the rotary liquid impactor separates particles using centrifugal force.

[0137] A Rotating Liquid Impactor is a device that captures fine particles suspended in the air into a liquid and separates them based on their size or mass. It utilizes rotational force, specifically centrifugal force, to collect particles into a liquid and filter out particles of a desired size. Rotating Liquid Impactors are used to filter airborne particles or prepare samples for analysis, and are particularly effective for capturing fine particles such as airborne viruses.

[0138] The composition of the Rotating Liquid Impactor is as follows.

[0139] (1) Rotating Disc: The rotating disc is a core component of the Rotating Liquid Impactor and is a disc that rotates at high speed. Particles suspended in PBS are separated according to size and mass as they strike the surface of the disc. The rotating disc separates particles using centrifugal force and effectively separates large and small particles through contact between the liquid and the particles. When particles suspended in PBS strike the rotating disc, they are separated by size and move along specific paths.

[0140] (2) Inlet System: The inlet system is a system that introduces a liquid mixture of PBS eluent and particles into a rotating disc. The mixture is injected into the rotating disc at a constant speed, and the particles are separated by the rotating disc. The inlet system controls the inlet speed and volume of the liquid to ensure that the mixture reaches the rotating disc in appropriate proportions. This allows the particles to be properly separated and captured.

[0141] (3) Particle separation system: The particle separation system uses centrifugal force to separate particles suspended in PBS according to size and mass. Large particles are pushed outward, while small particles remain in the center and are separated. The particle size is separated according to the speed and structure of the rotating disk, and the separated particles are moved to the sample collection unit and the waste collection unit.

[0142] (4) Sample Collection Unit: The sample collection unit collects liquid containing small particles suspended in PBS eluent. Liquid separated according to particle size and mass is collected in the sample collection unit and transferred to the detection sensor. The sample collection unit collects PBS mixtures containing only small particles, and the liquid is subsequently sent to the virus detection sensor.

[0143] (5) Waste Treatment System: Liquids containing large particles are treated as waste. Large particles that are separated from the entire disk and pushed outward flow into the waste collection unit. The waste collection unit collects and processes the large particles and some PBS mixture, and discharges them through the waste treatment system if necessary.

[0144] The operating principle of the Rotating Liquid Impactor is as follows.

[0145] (1) Inflow of mixture: A mixture of particles suspended in PBS eluent is injected into the rotating disk through the inflow system. The liquid spreads over the surface of the rotating disk, and the particles come into contact with the disk surface.

[0146] (2) Particle separation by centrifugal force: As the rotating disk rotates at high speed, centrifugal force is generated. Due to this centrifugal force, large particles are pushed outward from the disk, while small particles remain near the center. In this process, particles are separated by size.

[0147] (3) Particle Collection and Separation: Particles attached to the disc are separated according to size and mass. Large particles are pushed to the outer edge of the disc and moved to the waste collection section, while small particles remain in the center and are collected in the sample collection section. At this time, the PBS eluent is also distributed.

[0148] (4) Sample Collection and Provision: A PBS mixture containing small particles is transferred to a sample collection unit and then sent to a detection sensor. During this process, the sample collection unit provides a liquid containing only unfiltered small particles.

[0149] (5) Waste treatment: PBS eluent mixed with large particles is transferred to a waste collection unit for treatment. If necessary, the waste can be discharged or treated for reuse.

[0150] The fine control of the Rotating Liquid Impactor is as follows.

[0151] (1) Rotation speed control: By adjusting the speed of the rotating disk, particles can be separated accurately by size. When rotating quickly, large particles are separated more effectively, and when rotating slowly, small particles are captured better.

[0152] (2) Liquid Inflow Control: The amount and speed of the mixture injected through the liquid inflow system can be controlled. If the injection volume is too high, particles may not be sufficiently captured, so the inflow must be carried out at an appropriate ratio.

[0153] The rotary liquid impactor is a device suitable for processing mixtures in which particles are suspended in PBS, separating particles by size through centrifugal force. This allows the PBS mixture containing only small particles to be sent to a sample collection unit, while large particles are treated as waste. This device is effective for processing not only airborne particles but also particles suspended in liquids.

[0154] The sample collection unit (1206) serves to collect filtered liquid samples and deliver them to a virus detection sensor. The sample collection unit (1206) collects liquid samples that have been purified through a filtering process and then supplies them to the virus detection sensor. During this process, the concentration of the sample must be optimized, and a sufficient amount required for detection is collected. The sample collection unit (1206) includes a device capable of controlling the volume and concentration of the liquid sample, thereby providing the sample required for virus detection in an optimal state.

[0155] The waste collection unit (1207) is a component that collects waste remaining after filtering, namely large particles or impurities. The waste collection unit (1207) serves to collect and process the large particles or impurities remaining after filtering. This waste is properly processed so as not to interfere with the analysis. The waste collection unit (1207) is designed to collect and dispose of the used eluent and impurities, and is managed to prevent cross-contamination.

[0156] The fluid control device (1208) is a device that controls the flow of the eluent, deionized water, ethanol, and sample liquid, and plays a key role in the system's coordination. The fluid control device (1208) controls the injection amount of the eluent, deionized water, ethanol, etc., and controls the flow of liquid distributed to the sample collection unit and the waste collection unit. Through this, the concentration of the sample is accurately controlled, and the optimal sample required for detection is provided. The fluid control device (1208) receives real-time data from the sensor to adjust the flow of the eluent and sample, ensuring that each liquid is injected at an accurate ratio. This allows the quality and concentration of the liquid sample to be consistently maintained.

[0157] The fluid control device (1208) is a key device that precisely controls the flow of various liquids, such as eluent, deionized water, and ethanol, in the elution device to ensure optimal performance of the system. This device regulates the flow of liquids, distributes liquids to the sample collection unit and waste collection unit as needed, and controls the amount and ratio of liquids required at each injection stage.

[0158] The fluid control device (1208) is composed of various elements, which allow for fine control of the flow of liquid within the system. The basic components are as follows.

[0159] (1) Fluid pump: A pump is used to transfer each liquid (eluent, deionized water, ethanol, etc.) to a collection surface or filtering device. The pump controls the flow rate of the liquid and is precisely designed to provide various injection speeds.

[0160] (2) Valve System: Valves used to block or open fluid flow regulate the flow of liquid at various locations. This is used to control the distribution of liquid to the sample collection unit and waste collection unit, or to regulate the mixing ratio of the eluent and deionized water.

[0161] (3) Flow meter: A flow meter is a device that measures the speed or amount of each liquid flowing. Through this, it detects in real time whether the liquid is flowing accurately according to the set amount and provides feedback.

[0162] (4) Sensor System: The fluid control device includes various sensors (particle sensors, pressure sensors, flow sensors, etc.) to monitor the flow of each liquid and its effects in real time. Based on this data, the processor can precisely control the liquid flow.

[0163] The operating principle of the fluid control device (1208) is basically to control the liquid flow through a processor and a sensor, and to regulate the eluent, deionized water, ethanol, etc., in the required ratio. The operation process is as follows.

[0164] (1) Data collection from sensors: Sensors such as particle sensors and flow meters detect liquid flow, particle concentration, size, etc. at each stage. Through this data, the fluid control device determines the volume and flow rate of each liquid.

[0165] (2) Processor control command: Based on the collected sensor data, the processor issues a command to adjust the flow of the liquid. For example, if the concentration of sample particles is lower than the standard, it issues a command to increase the injection amount of the eluent or adjust the ratio of deionized water.

[0166] (3) Pump and valve operation: According to the command of the processor, the fluid pump injects the required amount of liquid, and the valve system adjusts the flow of the liquid to the correct path. The pump controls the flow rate, and the valve opens or closes the flow of liquid to distribute the liquid to the sample collection unit and the waste collection unit.

[0167] (4) Real-time feedback control: By receiving real-time feedback from flow meters and sensors, the processor can continuously check and adjust whether the flow is proceeding as expected. If the eluent is injected too fast or too slow, the pump speed is finely adjusted to correct this.

[0168] The fluid control device (1208) utilizes real-time communication and a feedback loop between the sensor and the processor to control a very precise liquid flow. The method for finely adjusting the liquid flow through this is as follows.

[0169] (1) Flow rate control: The fluid flow rate can be adjusted very precisely through a combination of a pump and a flow meter. The flow meter measures the amount and speed of the liquid injected by the pump in real time and provides feedback to the processor so that it can be adjusted. This feedback is repeated in very short cycles, and even small errors are adjusted immediately.

[0170] (2) Mixing Ratio Control: The ratio of the eluent to the deionized water is adjusted by a fluid control device, which may vary depending on the particle concentration or sample condition. Based on the particle size and concentration detected by the sensor, the processor adjusts the pump speed and the degree of valve opening so that the two liquids are mixed at the desired ratio.

[0171] (3) Pressure and flow rate balance control: It is also important to balance the pressure within the system to ensure smooth fluid flow. Each valve and pump operates in synchronization to maintain the set pressure and flow rate, and pressure sensors detect when this balance is disrupted and take immediate action.

[0172] A specific example of the operation of the fluid control device (1208) is as follows.

[0173] (1) Sample collection and waste distribution: The fluid control device adjusts the amount of liquid sample according to the size and concentration of sample particles. If the sensor detects that the concentration of sample particles is high, it sends more eluent to the sample collection unit and reduces the amount of liquid sent to the waste collection unit. Conversely, if the sample concentration is low, it injects more deionized water to dilute it and then discharges a portion to the waste collection unit.

[0174] (2) Cleaning and Disinfection Process: During the cleaning and disinfection stage, the fluid control device precisely controls the flow of deionized water and ethanol to thoroughly clean and disinfect the collection surface. This prevents cross-contamination and increases the reusability of the system.

[0175] The fluid control device (1208) is a key device that precisely controls the flow of liquids such as eluent, deionized water, and ethanol through a real-time feedback loop of a sensor and a processor. This system, composed of a pump, valve, and flow meter, maintains virus samples captured from the air in an optimized state and efficiently controls the amount and ratio of liquid to increase detection sensitivity.

[0176] The buffer injection device (1209) is a device for accurately and uniformly mixing a lysis buffer into a liquid sample generated through a filtering process. This device precisely controls the mixing ratio of the sample and the buffer to maximize virus detection sensitivity and automates the chemical processing of the sample. The buffer injection device includes a fluid control system, such as a metering pump, to adjust the injection volume and flow rate according to the concentration of the sample and analysis conditions. This allows for the efficient extraction of viral proteins and nucleic acids (RNA / DNA) from the sample, preparing them in an optimized state before delivery to a virus detection sensor.

[0177] The operation of the elution device (1200) is as follows. Particles captured by the cyclone device (1100) are collected on the collection surface (1201) of the elution device (1200) and are converted into a liquid sample by being suspended in the eluent injected through the eluent injection device (1202). Subsequently, large particles are sent to the waste collection unit (1207) via the filtering device (1205), and small particles are collected in the sample collection unit (1206). The liquid sample collected in the sample collection unit (1206) is transferred to a virus detection sensor for analysis. During this process, a lysis buffer is mixed into the sample via the buffer injection device (1209) to extract virus proteins and nucleic acids, thereby maximizing detection sensitivity. After the operation is completed, the collection surface is washed via the deionized water injection device (1203), and the system is disinfected via the ethanol injection device (1204) to prevent cross-contamination. The elution device (1200) is designed as a high-efficiency system that effectively captures viruses in the air and converts them into an optimized liquid sample.

[0178] The control unit (1300) includes a transceiver (1301), a memory (1302), a processor (1303), an input device (1304), and an output device (1305). The control unit (1300) controls the cyclone device (1100), the elution device (1200), and the air discharge device (1400), and plays an important role in coordinating the operation of the entire system, such as liquid flow, filtering, and sample distribution.

[0179] The transceiver (1301) is responsible for transmitting and receiving data between the control device and an external system or internal components. The transceiver (1301) receives data from various sensors and external devices and functions to transmit commands processed by the processor to other components. The transceiver (1301) collects real-time data from sensors and transmits it to the processor. In addition, it plays an important role in controlling the liquid sample generation device (1000) by transmitting commands processed by the processor (1303) to the eluent injection device, fluid control device, particle sensor, etc. Through the transceiver (1301), the control device (1300) receives particle size or concentration from the sensor in real time and issues commands to adjust the eluent injection amount, buffer injection amount, etc.

[0180] The memory (1302) is a device that stores data and commands processed by the control device (1300). It serves to temporarily store data collected in real time or to record commands and setting values ​​that can be preserved for a long period. The memory (1302) stores data necessary for the liquid sample generating device (1000) to operate normally and allows the processor (1303) to access the necessary data at any time. It records operational data such as particle size, concentration data, and the injection speed and ratio of each liquid collected in real time, so that the processor (13030) can analyze and process them whenever necessary. The memory (1302) stores set reference values ​​(e.g., injection ratio of eluent, particle size reference value, buffer liquid injection amount, etc.) and processes them by comparing them with data detected in real time.

[0181] The processor (1303) acts as the brain of the control device, processes and analyzes collected data, and serves as the central hub for issuing various control commands. It analyzes data transmitted from sensors to issue commands for necessary adjustments or performs system control based on user input. Based on sensor data, the processor (1303) controls the operation of the liquid sample generating device (1000) and controls the eluent injection amount, particle filtering, buffer injection amount, and cleaning and disinfection processes in real time. The processor (1303) also compares real-time data with reference values ​​stored in memory to adjust the eluent concentration or manages the liquid sample generating device (1000) to operate efficiently. When the particle concentration is higher than the set reference, the processor (1303) issues a command to increase the eluent injection amount or send more liquid to the sample collection unit.

[0182] The input device (1304) is an interface that allows a user to input commands into the system or change setting values. Through the input device, the user can control various operations of the system or set the eluent injection ratio, cleaning cycle, disinfection cycle, etc. The input device (1304) is configured in the form of a keypad, touchscreen, or button, and through this, the user can control the system or set parameters. The values ​​set by the user are transmitted to the processor and used to adjust the system operation. Through the input device (1304), the user can manually set the eluent injection ratio or change the filtering cycle. Additionally, through the input device (1304), the user can adjust the cleaning and disinfection cycles according to specific sample conditions.

[0183] The output device (1305) is a device that displays the status of the liquid sample generating device (1000), sensor data, processing results of the processor, etc., to the user. It allows the current status of the liquid sample generating device (1000) to be visually checked and also serves to convey warning or error messages. The output device (1305) is configured in the form of an LED display, an LCD panel, or a warning light, and displays the status or result data of the liquid sample generating device (1000) in real time. Through this, the user can check whether the liquid sample generating device (1000) is operating properly or if a problem has occurred. The output device (1305) displays the current particle concentration, eluent injection amount, buffer injection amount, and system warnings (e.g., filter clogging, eluent shortage, etc.) to the user in real time. In addition, data is output that allows verification of whether the liquid sample generating device (1000) is operating correctly.

[0184] The control device (1300) collects real-time data from a sensor, and based on the data, the processor determines the optimal liquid flow, eluent concentration, cleaning and disinfection cycle, etc., to control the liquid sample generating device (1000). It exchanges data with an external device through a transceiver and checks whether the liquid sample generating device (1000) is operating normally by comparing it with a reference value stored in memory. The user can control the liquid sample generating device (1000) or change setting values ​​through an input device, and can check the status of the liquid sample generating device (1000) through an output device.

[0185] The operation process of the control device (1300) is as follows.

[0186] (1) Data collection: Data collected from particle sensors, flow meters, etc. is transmitted to the processor via a transmitter / receiver.

[0187] (2) Data analysis and processing: The processor compares the collected data with reference values ​​in memory and issues appropriate control commands.

[0188] (3) Liquid flow control: The eluent injection device and the fluid control device control the liquid flow according to the processor's command.

[0189] (4) Real-time feedback: Transmits real-time data from the system to an output device so that the user can check the status of the system.

[0190] (5) User control: The user can control the operation of the system or set specific values ​​through an input device.

[0191] The control device (1300) plays an essential role in stably and precisely managing the entire liquid sample generation device (1000), and each component interacts to optimize the performance of the airborne virus detection system.

[0192] The air exhaust device (1400) includes an air outlet (1401) and an air purification filter device (1402). The air exhaust device (1400) performs the function of purifying the air used inside the virus detection system and safely discharging it to the outside. This prevents uncaptured fine particles, residual viruses, or other harmful substances from leaking out during the airflow within the system, and ensures the safety of the working environment and the reliability of the system. The air exhaust device is composed of an air purification filter device (1402) that removes particles from the air and an air outlet (1401) that smoothly discharges the purified air to the outside, thereby efficiently integrating the purification and discharge processes.

[0193] The air outlet (1401) is designed as a passage for safely discharging purified air to the outside. This component controls the direction and flow of the air discharge and includes a backflow prevention device to prevent contaminated air from flowing back. The outlet is designed to optimize the speed and direction of the airflow to increase discharge efficiency and ensure uniform discharge to the external environment. Additionally, sound-absorbing structures may be additionally applied to reduce noise during the discharge process, thereby improving the convenience of the working environment.

[0194] The air purification filter device (1402) is a key component for purifying air within a virus detection system. The air purification filter device (1402) includes a high-efficiency particulate air (HEPA) filter and an activated carbon filter to remove fine particles, viruses, and harmful substances from the air. The HEPA filter provides the ability to filter out more than 99.97% of nanometer-sized fine particles, and the activated carbon filter improves air quality by adsorbing harmful gases. The purification filter is designed in a multi-stage manner to simultaneously perform particle removal and chemical purification processes, and is designed to facilitate periodic replacement and maintenance. The air purification filter device (1402) may further include a plasma air purification device that purifies air by generating plasma.

[0195]

[0196] According to various embodiments of the present invention, a liquid sample generating device for airborne viruses comprises: a cyclone device for generating an aerosol sample by removing particles from air; an elution device for generating a liquid sample from the aerosol sample; an air discharge device for discharging the air that has been introduced through the cyclone device and passed through the elution device to the outside; and a control device connected to the cyclone device, the elution device, and the air discharge device.

[0197] According to various embodiments of the present invention, the cyclone device comprises: one or more cyclones for filtering particles in the air; and one or more particle sensors for detecting particle characteristics of particles introduced into the cyclone device and particles filtered by the one or more cyclones. The one or more particle sensors include an inlet particle sensor at the inlet where air enters the cyclone device and one or more cyclone particle sensors corresponding to each of the one or more cyclones. If the one or more cyclones are multiple cyclones, the multiple cyclones are connected in series. Each of the multiple cyclones is configured to filter particles of different particle sizes.

[0198] According to various embodiments of the present invention, the elution device comprises: a collection surface which is a space in which the aerosol sample is collected and an eluent, which is phosphate buffered saline (PBS), is injected; an eluent injection device for injecting the eluent into the collection surface; a deionized water injection device for injecting deionized water (DI water) to control the concentration of the eluent injected into the collection surface by diluting the eluent into the collection surface; an ethanol injection device for injecting ethanol (EtOH) into the collection surface for disinfecting the collection surface; a filtering device for filtering particles larger than a predetermined size from the aerosol sample in the eluent or from the mixture of the eluent and the deionized water; a sample collection unit for collecting the liquid sample that has passed through the filtering device in the mixture; and a waste collection unit for collecting waste filtered by the filtering device in the mixture. It includes a buffer solution injection device for mixing a buffer solution, which is a lysis buffer, with the above liquid sample; and a fluid control device for controlling the flow of the eluent, the deionized water, the ethanol, the mixture, the liquid sample, and the buffer solution.

[0199] According to various embodiments of the present invention, the air discharge device comprises: an air purification filter device for purifying the air that has been introduced through the cyclone device and passed through the elution device; and an air outlet for discharging the air that has passed through the air purification filter to the outside.

[0200] According to various embodiments of the present invention, the control device comprises: a transceiver; a memory; an input device; an output device; and a processor functionally connected to the cyclone device, the elution device, the air discharge device, the transceiver, the memory, the input device and the output device.

[0201] According to various embodiments of the present invention, the processor may be configured to: determine the ratio and amount of the eluent and the deionized water to be injected into the collection surface according to the particle characteristics detected by the one or more particle sensors; and to inject the eluent, or the eluent and the deionized water, into the collection surface by the eluent injection device and the deionized water injection device based on the determined ratio and amount.

[0202] According to various embodiments of the present invention, the particle characteristics may include one or more of particle size, particle concentration, particle mass, particle number, and particle collection efficiency. The particle concentration may be the number of particles per unit volume. The particle mass may be the total mass of particles collected by the one or more cyclones. The particle number may be the total number of particles collected by the one or more cyclones. The particle collection efficiency may be the ratio of the number of particles collected by the one or more cyclones to the total number of particles entering the plurality of cyclones.

[0203] According to various embodiments of the present invention, based on the particle characteristics, the processor comprises: increasing the ratio of the eluent and decreasing the ratio of the deionized water when, among the particles captured by the one or more cyclones, the number of particles larger than a reference size is greater than the number of particles smaller than the reference size; increasing the amount of the eluent, increasing the ratio of the eluent, and decreasing the ratio of the deionized water when, among the particles captured by the one or more cyclones, the number of particles captured is higher than a reference mass when, the amount of the eluent, increasing the ratio of the eluent, and decreasing the ratio of the deionized water; and increasing the total number of particles captured by the one or more cyclones when, the number of particles captured is higher than a reference number when, the amount of the eluent, increasing the ratio of the eluent, and decreasing the ratio of the deionized water. Alternatively, if the particle capture efficiency is higher than the standard efficiency, the device may be further configured to increase the amount of the eluent, increase the ratio of the eluent, and decrease the ratio of the deionized water.

[0204] According to various embodiments of the present invention, the filtering device may be composed of one of a rotating liquid impactor or a membrane filter, or a combination of the rotating liquid impactor and the membrane filter. The rotating liquid impactor may be configured to rotate the mixture so that, depending on the size of the particles, large particles are captured in the liquid and small particles are discharged. The membrane filter may be configured so that, through the pores of the membrane filter, large particles are captured in the membrane filter and small particles are discharged. When the filtering device is composed of a combination of the rotating liquid impactor and the membrane filter, the filtering device may be configured such that the rotating liquid impactor filters particles relatively larger than the membrane filter, and then the membrane filter filters particles relatively smaller than the rotating liquid impactor.

[0205] According to various embodiments of the present invention, the mixture may be composed of a solid component and a liquid component. The solid component may include sample particles of a reference size or smaller that pass through the filtering device and are sent to the sampling collection unit, and waste particles exceeding the reference size that are filtered by the filtering device and sent to the waste collection unit. The liquid component may include the eluent injected into the collection surface, or the eluent and the deionized water. The liquid component may be distributed to the sample collection unit and the waste collection unit through a fluid distribution valve by the fluid control device based on a set distribution ratio.

[0206] According to various embodiments of the present invention, the distribution ratio may be based on the purity of the liquid sample or the efficient use of the eluent. If the distribution ratio is based on the purity of the liquid sample, the distribution ratio may be set so that the ratio of the liquid distributed to the sample collection unit is higher than the reference sample liquid ratio and the ratio of the liquid distributed to the waste collection unit is lower than the reference waste liquid ratio. If the distribution ratio is based on the efficient use of the eluent, the distribution ratio may be set so that the ratio of the liquid distributed to the sample collection unit is lower than the reference sample liquid ratio and the ratio of the liquid distributed to the waste collection unit is higher than the reference waste liquid ratio.

[0207] According to various embodiments of the present invention, the processor may be further configured to: clean the collection surface by injecting the deionized water into the collection surface by means of the deionized water injection device after the distribution of the mixture to the sample collection unit and the waste collection unit is completed; and disinfect the collection surface by injecting the ethanol into the collection surface by means of the ethanol injection device after the collection surface is cleaned.

[0208] According to various embodiments of the present invention, the processor may be further configured to discharge a set amount of the liquid sample from the sample collection unit to a virus detection sensor outside the liquid sample generating device by means of the fluid control device after the distribution of the mixture to the sample collection unit and the waste collection unit is completed.

[0209] According to various embodiments of the present invention, the sample collection unit may include a liquid particle sensor. The liquid particle sensor may be configured to detect one or more of the number or concentration of sample particles among the liquid samples within the sample collection unit. The concentration of the particles may be based on the volume of the liquid sample within the sample collection unit and the number of sample particles. The volume of the liquid sample may be based on the volume of the liquid composition distributed to the sample collection unit. The amount of sample provided may be determined by the processor based on the concentration of the sample particles. Based on whether the concentration of the sample particles is within a set reference sample concentration range, or is higher than or lower than the reference sample concentration range, the amount of sample provided may be set to a reference sample amount, set higher than the reference sample amount, or set lower than the reference sample amount. The degree to which the amount of sample provided is set higher or lower than the reference sample amount may be based on the ratio of the concentration of the sample particles to the median value of the reference sample concentration range.

[0210] Liquid particle sensors are devices that detect the size, number, and concentration of particles present in a liquid in real time, and are particularly used for accurately detecting and analyzing fine particles. Because these sensors can measure the characteristics of particles contained in liquids in various environments, they play an important role in virus detection systems, pharmaceuticals, life sciences, and industrial processes.

[0211] The configuration of the liquid particle sensor is as follows.

[0212] (1) Light source: Optical methods are used to detect particles in a liquid, and light is generally shone into the liquid using a light source such as a laser or LED. The light from the light source passes through the liquid and hits the particles, and the size or concentration of the particles is measured by utilizing the properties of scattering or absorption by the particles.

[0213] (2) Detector: A device that detects signals reflected or scattered after light emitted by a light source interacts with particles. The detector converts optical signals into electrical signals and analyzes the size and number of particles based on the degree to which the particles scatter or absorb light. High-sensitivity devices such as photodiodes or CCD sensors may be used.

[0214] (3) Flow Cell: A passage through which liquid flows, designed to pass between a light source and a detector. As the liquid passes through this passage, particles are irradiated by the light source and detected by the detector. The flow cell is made of a transparent material so that particles can interact accurately with the light, and is designed to allow particles to flow freely. Since the liquid must flow regularly within a fixed flow cell, a pump or valve to control the flow of the liquid may also be used.

[0215] (4) Electronic circuit section: This is a device that analyzes signals received from the detector and calculates the size, number, concentration, etc. of particles. It converts electrical signals based on the scattering intensity, size, and concentration of particles into digital signals for processing. A high-sensitivity circuit capable of detecting very small voltages or currents is required, and it processes this data to provide the final analysis results. This circuit section may also include a memory and communication device responsible for storing and transmitting data.

[0216] (5) Processor and Software: A central processing unit (CPU) and software linked to it used to process and interpret detected data. Data is processed in real time, and the size distribution, number, concentration, etc. of particles are calculated and results are provided to the user. The software visually displays the data through a graphical interface so that the user can easily understand it.

[0217] The operating principle of the liquid particle sensor is as follows.

[0218] (1) Light scattering and particle detection: When particles in a liquid interact with light emitted from a light source, the light is scattered or partially absorbed by the particles. This scattering of light depends on the size, number, and concentration of the particles. Larger particles scatter more light, while smaller particles scatter relatively less. The detector measures the amount of this scattered light to analyze the size and concentration of the particles.

[0219] (2) Particle Size Analysis: The detector measures the intensity of scattered light and estimates the particle size. It operates by generating strong signals for large particles and weak signals for small particles. Based on this, the sensor calculates the particle size distribution within the liquid. When particles of various sizes are present, the scattered signal differs for each size, thereby obtaining the distribution.

[0220] (3) Measurement of particle count and concentration: The number of particles present in the liquid can be calculated based on the frequency and intensity of the detected signal. If the intensity of the scattered light originates from particles of a certain size, the number of particles of that size can be estimated by measuring the frequency of the signal. Concentration is defined as the number of particles per unit volume, and the concentration can be calculated by comparing the volume of the liquid passing through the channel with the number of particles.

[0221] (4) Real-time data processing: The electronic circuit and processor process the detection data in real time to calculate the particle size, number, and concentration in the liquid and provide the results to the user. If necessary, the data may be stored or transmitted to an external system via a communication device.

[0222] The operation process of the liquid particle sensor is as follows.

[0223] (1) Liquid flow inflow: Liquid flows through the channel, and particles flowing within the channel are irradiated by a light source.

[0224] (2) Light scattering: As particles contained in the liquid interact with light, scattered light is generated, and the detector detects this in real time.

[0225] (3) Signal conversion: The scattered light is converted into an electronic signal, and the size and number of particles are measured according to their intensity and frequency.

[0226] (4) Data processing: The electronic circuit and processor process the signal in real time to calculate the size, number, and concentration of the particles and provide the data to the user.

[0227] Liquid particle sensors can be utilized in various fields. For example:

[0228] (1) Virus detection: Used in virus detection systems to measure the size and concentration of virus particles suspended in PBS in real time.

[0229] (2) Pharmaceutical industry: Plays an important role in detecting and controlling impurities in liquids during pharmaceutical processes.

[0230] (3) Life science: Used to analyze biological samples such as cells, viruses, and protein particles.

[0231] (4) Water quality monitoring: Used to monitor water quality and detect fine impurities.

[0232] Liquid particle sensors are devices capable of precisely measuring the size, number, and concentration of particles contained within a liquid, analyzing particle characteristics in real time using a light source and a detector. They play a crucial role in various fields, such as virus detection, pharmaceutical process control, and water quality monitoring, and are particularly useful for accurately detecting and analyzing fine particles.

[0233]

[0234] FIG. 3 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.

[0235] FIG. 3 illustrates a side view of the airborne virus detection device of the present invention, showing the main components of the device. The cyclone device separates and collects airborne particles by size, and the collected particles are transferred to an elution device.

[0236] The elution device serves to convert virus particles delivered from the cyclone device into a liquid sample. This device includes a collection surface and an eluent injection device, and maximizes virus detection sensitivity through the purification of the liquid sample.

[0237] The collection surface is a key component where virus particles gather, and the viruses are eluted through interaction with the eluent. The liquid sample generated through this process is transferred to the sample collection unit to complete preparation for detection.

[0238] The air outlet of the virus detection device discharges purified air to the outside and prevents airborne contaminants from leaking out. The air outlet is designed to maintain a smooth airflow.

[0239]

[0240] FIG. 4 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.

[0241] Figure 4 illustrates another side view of the airborne virus detection device and highlights the system integration design including the control unit. The control unit manages all components of the device in an integrated manner.

[0242] The cyclone device separates particles of different sizes in the air in stages, ensuring that each separated particle is delivered to an elution device. This enables the efficient processing of various particle sizes in the air.

[0243] Multiple injection devices are positioned at the top of the elution unit, supporting sample preparation and device disinfection through the injection of eluent, deionized water, ethanol, and other substances, respectively. This process contributes to preventing cross-contamination and maintaining sample quality.

[0244] The control unit interacts with the user through a touch screen display, enabling real-time monitoring and operation of the status of each component. This enhances the efficiency and ease of use of the device.

[0245]

[0246] FIG. 5 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.

[0247] FIG. 5 illustrates a top view of the airborne virus detection device of the present invention and shows the physical arrangement of all components at a glance. The interconnection of the cyclone device, the elution device, and the air outlet is clearly shown.

[0248] A cyclone device is a centrifugal force-based multi-stage system for the separation and capture of airborne particles, where capture is performed according to particle size at each stage. This plays a key role in increasing detection sensitivity.

[0249] The upper part of the elution device is equipped with various injection devices, each of which supports the elution process as well as the cleaning and disinfection processes. This ensures the continuous performance maintenance of the elution device.

[0250] The air outlet discharges purified air to the outside in conjunction with the air purification filter device, maintaining the safety and environmental friendliness of the virus detection device. The outlet smoothly regulates the flow of purified air, providing overall system stability.

[0251]

[0252] FIG. 6 illustrates an example of an elution device in an airborne virus detection device implemented according to an embodiment of the present invention.

[0253] FIG. 6 is an example illustrating the main components of an elution device in an airborne virus detection device according to an embodiment of the present invention. The elution device serves to convert viruses captured in the air into a liquid sample and includes various injection and collection devices for purifying the sample and preparing it for analysis.

[0254] The eluent injection device is a core component of the elution system that injects an eluent (PBS) onto the collection surface to suspend captured virus particles in the liquid. This converts the viruses into a liquid state, ensuring uniform sample distribution and high precision.

[0255] The deionized water injection device and the ethanol injection device are key components that support the cleaning and disinfection processes of the elution device. Deionized water cleans the collection surface and the interior of the system, while ethanol is used as a disinfectant to prevent contamination within the device and minimize cross-contamination.

[0256] The waste collection unit collects filtered large particles or used eluents for safe disposal, while the sample collection unit gathers purified liquid samples and delivers them to a virus detection sensor. This design is configured to efficiently support waste disposal while maintaining sample quality.

[0257]

[0258] The embodiments described above are combinations of the components and features of the present invention in a specific form. Each component or feature should be considered optional unless otherwise explicitly stated. Each component or feature may be implemented in a form not combined with other components or features. Additionally, it is possible to construct embodiments of the present invention by combining some components and / or features. The order of operations described in the embodiments of the invention may be changed. Some components or features of one embodiment may be included in another embodiment, or may be replaced with corresponding components or features of another embodiment. It is obvious that embodiments may be constructed by combining claims that do not have an explicit citation relationship in the claims, or that they may be included as new claims through amendments made after filing.

[0259] It will be apparent to those skilled in the art that the present invention may be embodied in other forms without departing from the technical spirit and essential features of the present invention. Accordingly, the above embodiments should be considered in all illustrative aspects rather than as a limiting one. The scope of the present invention shall be determined by a reasonable interpretation of the appended claims and all possible variations within the equivalent scope of the present invention.

[0260]

[0261] The present invention relates to an apparatus for generating a liquid sample for airborne viruses. Specifically, the present invention relates to an apparatus for generating a liquid sample by eluting an aerosol sample from air, in order to provide a liquid sample for airborne viruses to a virus detection sensor.

Claims

1. In a liquid sample generating device for airborne viruses, A cyclone device for generating an aerosol sample by removing particles from air; An elution device for generating a liquid sample from the above aerosol sample; An air discharge device for discharging the air to the outside after it has been introduced through the cyclone device and passed through the elution device; It includes a control device connected to the above-mentioned cyclone device, the above-mentioned elution device, and the above-mentioned air discharge device, and The cyclone device comprises: one or more cyclones for filtering airborne particles; and one or more particle sensors for detecting particle characteristics of particles introduced into the cyclone device and particles filtered by the one or more cyclones; wherein the one or more particle sensors include an inlet particle sensor at the inlet where air enters the cyclone device and one or more cyclone particle sensors corresponding to each of the one or more cyclones; and where the one or more cyclones are multiple cyclones, the multiple cyclones are connected in series, and the multiple cyclones are each configured to filter particles of different particle sizes. The above elution device comprises: a collection surface, which is a space where the aerosol sample is collected and an eluent, which is phosphate buffered saline (PBS), is injected; an eluent injection device for injecting the eluent into the collection surface; a deionized water injection device for injecting deionized water (DI water) to control the concentration of the eluent injected into the collection surface by diluting the eluent into the collection surface; an ethanol injection device for injecting ethanol (EtOH) into the collection surface for disinfecting the collection surface; a filtering device for filtering particles larger than a predetermined size from the aerosol sample in the eluent or from a mixture in which the eluent and the deionized water are eluted; a sample collection unit for collecting the liquid sample that has passed through the filtering device in the mixture; a waste collection unit for collecting waste filtered by the filtering device in the mixture; and a buffer injection device for mixing a buffer solution, which is a lysis buffer, with the liquid sample. A fluid control device for controlling the flow of the above eluent, the above deionized water, the above ethanol, the above mixture, the above liquid sample, and the above buffer solution; comprising The above air discharge device comprises: an air purification filter device for purifying the air that has been introduced through the cyclone device and passed through the elution device; and an air outlet for discharging the air that has passed through the air purification filter to the outside. The above control device comprises: a transceiver; a memory; an input device; an output device; and a processor functionally connected to the cyclone device, the elution device, the air discharge device, the transceiver, the memory, the input device, and the output device. Liquid sample generation device.

2. In Paragraph 1, The above processor is: Determining the ratio and amount of the eluent and the deionized water injected into the collection surface according to the particle characteristics detected by the one or more particle sensors; Configured to inject the eluent, or the eluent and the deionized water, into the collection surface based on the above-determined ratio and amount by means of the eluent injection device and the deionized water injection device. Liquid sample generation device.

3. In Paragraph 2, The above particle characteristics include one or more of particle size, particle concentration, particle mass, particle number, and particle capture efficiency, and The above particle concentration is the number of particles per unit volume, and The above particle mass is the total mass of particles captured by the one or more cyclones, and The above number of particles is the total number of particles captured by the one or more cyclones, and The particle capture efficiency is the ratio of the number of particles captured by one or more cyclones to the total number of particles that entered the plurality of cyclones, Liquid sample generation device.

4. In Paragraph 3, Based on the above particle characteristics, the processor: If, among the particles captured by the one or more cyclones, particles larger than a reference size are more numerous than particles smaller than a reference size, the ratio of the eluent is increased and the ratio of the deionized water is decreased; If the particle concentration of particles captured by the above one or more cyclones is higher than the reference concentration, increase the amount of the eluent, increase the ratio of the eluent, and decrease the ratio of the deionized water; If the total mass of particles captured by the above one or more cyclones is higher than the reference mass, the amount of the eluent is increased, the ratio of the eluent is increased, and the ratio of the deionized water is decreased; If the total number of particles captured by the above one or more cyclones is higher than the reference number, increase the amount of the eluent, increase the ratio of the eluent, and decrease the ratio of the deionized water; or, If the particle capture efficiency is higher than the standard efficiency, the above is further configured to increase the amount of the eluent, increase the ratio of the eluent, and decrease the ratio of the deionized water. Liquid sample generation device.

5. In Paragraph 1, The filtering device is composed of one of a rotating liquid impactor or a membrane filter, or a combination of the rotating liquid impactor and the membrane filter. The above rotary liquid impactor is configured to rotate the mixture so that, depending on the size of the particles, large particles are captured in the liquid and small particles are discharged. The above membrane filter is configured such that large particles are captured by the membrane filter and small particles are discharged through the pores of the membrane filter, and When the filtering device is composed of a combination of the rotary liquid impactor and the membrane filter, the filtering device is configured such that the rotary liquid impactor filters particles relatively larger than the membrane filter, and then the membrane filter filters particles relatively smaller than the rotary liquid impactor. Liquid sample generation device.

6. In Paragraph 1, The above mixture consists of a solid composition and a liquid composition, and The above solid composition includes sample particles of a reference size or smaller that pass through the filtering device and are sent to the sampling collection unit, and waste particles exceeding the reference size that are filtered by the filtering device and sent to the waste collection unit. The above liquid composition comprises the eluent injected into the collection surface, or the eluent and the deionized water, and The above liquid composition is distributed to the sample collection unit and the waste collection unit through the fluid distribution valve by the fluid control device based on a set distribution ratio, Liquid sample generation device.

7. In Paragraph 6, The above distribution ratio is based on the purity of the liquid sample or the efficient use of the eluent, and If the above distribution ratio is based on the purity of the liquid sample, the distribution ratio is set to increase the ratio of the liquid distributed to the sample collection unit above the reference sample liquid ratio and to decrease the ratio of the liquid distributed to the waste collection unit below the reference waste liquid ratio, and When the above distribution ratio is based on the efficient use of the above eluent, the distribution ratio is set such that the ratio of the liquid distributed to the sample collection unit is lower than the reference sample liquid ratio and the ratio of the liquid distributed to the waste collection unit is higher than the reference waste liquid ratio. Liquid sample generation device.

8. In Paragraph 6, The above processor is: After the distribution of the above mixture to the above sample collection unit and the above waste collection unit is completed, the collection surface is cleaned by injecting the deionized water into the collection surface by the above deionized water injection device; After the above-mentioned collection surface is washed, the collection surface is further configured to be disinfected by injecting the ethanol into the collection surface by the ethanol injection device. Liquid sample generation device.

9. In Paragraph 6, The above processor is: After the distribution of the above mixture to the above sample collection unit and the above waste collection unit is completed, the fluid control device is further configured to discharge a set amount of the provided sample among the liquid samples in the above sample collection unit to a virus detection sensor outside the liquid sample generating device. Liquid sample generation device.

10. In Paragraph 9, The above sample collection unit includes a liquid particle sensor, and The liquid particle sensor is configured to detect one or more of the number or concentration of sample particles among the liquid samples in the sample collection unit, and The concentration of the above particles is based on the volume of the liquid sample within the sample collection unit and the number of the sample particles, and The volume of the above liquid sample is based on the volume distributed to the sample collection part of the above liquid composition, and The amount of the sample provided above is determined by the processor based on the concentration of the sample particles, and Based on whether the concentration of the sample particles is within a set reference sample concentration range, or is higher than or lower than the reference sample concentration range, the provided sample amount is set to a reference sample amount, or is set higher than or lower than the reference sample amount, and The degree to which the provided sample amount is set higher or lower than the reference sample amount is based on the ratio of the concentration of the sample particles to the median value of the reference sample concentration range. Liquid sample generation device.

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