Liquid path system and selector valve

Abstract

The present application discloses a liquid path system and a selector valve. The liquid path system comprises a first selector valve, a chip carrier, and a driving member. The first selector valve comprises a plurality of first liquid path ports, at least one second liquid path port, and a plurality of third liquid path ports. Since the first selector valve comprises the plurality of first liquid path ports, the at least one second liquid path port, and the plurality of third liquid path ports, and the first selector valve can be switched such that the plurality of first liquid path ports are connected to the plurality of third liquid path ports in one-to-one correspondence, so as to establish communication for a plurality of mutually independent sample channels, the liquid path system can realize one-to-one connection between the plurality of independent sample channels and a plurality of fluid channels on the chip carrier by switching the first selector valve, so that the plurality of fluid channels for sequencing slides can achieve separate and independent sample loading, thereby greatly improving the sample pooling efficiency, shortening the sample pooling time, and achieving immediate testing upon sample arrival.

Description

A hydraulic system and a selector valve

[0001] This application claims priority to the patent application filed on January 7, 2025, with application number 202510025193.4 and entitled "A hydraulic system and a selector valve". Technical Field

[0002] This application relates to the field of gene sequencing technology, specifically to a liquid circuit system and a selection valve. Background Technology

[0003] High-throughput sequencing platforms typically use multi-lane sequencing slides. After the samples are processed, they are manually loaded onto the sequencing slides by lanes outside the sequencing platform or using an additional sample loader.

[0004] In existing technologies, high-throughput sequencing platforms are limited by sample size and low-throughput applications. When products are released, they provide sequencing slides with various throughputs. At the same time, during use, it is necessary to collect samples, that is, to accumulate a certain number of samples before starting sequencing, in order to reduce sequencing costs.

[0005] However, the current sequencing slides have insufficient throughput and low granularity, and the sample collection time is long, making it difficult to achieve immediate testing upon sample arrival.

[0006] Application content

[0007] This application provides a liquid circuit system and a selection valve to address the problems of long sample collection time and difficulty in achieving immediate sample testing upon arrival in current sequencing processes.

[0008] In some embodiments, a fluid circuit system is provided, comprising:

[0009] The first selection valve includes a plurality of first liquid passage ports of the first selection valve, at least one second liquid passage port of the first selection valve, and a plurality of third liquid passage ports of the first selection valve. The first selection valve can be switched to connect the plurality of first liquid passage ports of the first selection valve to the plurality of third liquid passage ports of the first selection valve in a one-to-one correspondence to conduct multiple independent sample channels. The first selection valve can also be switched to connect the second liquid passage port of the first selection valve to one or more of the plurality of third liquid passage ports of the first selection valve to conduct at least one reagent channel.

[0010] A chip stage, the chip stage including a plurality of first liquid passage ports and a plurality of second liquid passage ports of the chip stage, the chip stage being used to place a sequencing slide, the sequencing slide having one or more fluid channels, one end of the fluid channel being connected to a first liquid passage port of the chip stage, and the other end of the fluid channel being connected to a second liquid passage port of the chip stage;

[0011] A driving element is connected to the second liquid channel port of the plurality of chip stages. The driving element is used to drive the sample or reagent through the first selection valve into the sequencing wafer on the chip stage.

[0012] In some embodiments, the first selection valve includes a stator and a rotor, and a first liquid passage port, a second liquid passage port, and a third liquid passage port of the first selection valve are disposed on the stator; the rotor is provided with multiple sample channels and at least one reagent channel, the sample channel having a first connection port and a second connection port, and the reagent channel having a first connection port and a second connection port.

[0013] The first connection port of the sample channel is used to switch to connect with the first liquid circuit port of the first selection valve, and the second connection port of the sample channel is used to switch to connect with the third liquid circuit port of the first selection valve.

[0014] The first connection port of the reagent channel is used to switch to connect to the second liquid path port of the first selector valve, and the second connection port of the reagent channel is used to switch to connect to the third liquid path port of the first selector valve.

[0015] In some embodiments, the rotor includes a first rotor and a second rotor, the first rotor and the second rotor being fixedly connected, the first rotor being located between the stator and the second rotor; the first rotor has a first surface facing the stator and a second surface facing away from the stator, the reagent channel being located on the first surface of the first rotor, the sample channel being located on the second surface of the first rotor, and a first connection port and a second connection port of the sample channel extending to the first surface of the first rotor; the stator has a first surface facing away from the first rotor and a second surface facing away from the first rotor, and a first liquid passage port, a second liquid passage port, and a third liquid passage port of the first selection valve extending from the first surface of the stator to the second surface of the stator.

[0016] In some embodiments, the first connection port of the sample channel, the second connection port of the sample channel, and the second connection port of the reagent channel are distributed at intervals along a first circumferential trajectory on a first surface of the first rotor. The first connection port of the reagent channel is located at the center of the first circumferential trajectory, and the reagent channel is distributed radially along the first circumferential trajectory. The first liquid passage port and the third liquid passage port of the first selection valve are distributed at intervals along a second circumferential trajectory on a second surface of the stator. The second liquid passage port of the first selection valve is located at the center of the second circumferential trajectory, and the first circumferential trajectory and the second circumferential trajectory are aligned and coincident in the axial direction of the rotor.

[0017] In some embodiments, the second connection port of the reagent channel is an arc-shaped strip structure distributed along the first circumferential trajectory, and the second connection port of the reagent channel is used to switch to connection with a third liquid passage port of one or more of the first selection valves.

[0018] In some embodiments, the first selection valve further includes a housing and a drive unit, the stator and the rotor are located inside the housing, the stator is fixedly connected to the housing, the rotor is rotatably connected to the housing, the output end of the drive unit is fixedly connected to the rotor, and the drive unit is used to drive the rotor to rotate relative to the stator.

[0019] In some embodiments, a second selector valve is further included, the second selector valve including a plurality of first liquid passage ports of the second selector valve and at least one second liquid passage port of the second selector valve, the second liquid passage port of the second selector valve being connected to the second liquid passage port of the first selector valve; the second selector valve is used to switch to one or more connections between the first liquid passage ports of the second selector valve and the second liquid passage ports of the second selector valve.

[0020] In some embodiments, the drive includes a plurality of unit pumps, each unit pump including a first liquid passage port and a second liquid passage port of the unit pump, wherein the first liquid passage port of each unit pump is connected to the second liquid passage port of the chip stage.

[0021] In some embodiments, a plurality of sample pools are also included, the plurality of sample pools being connected to a first liquid passage port of one or more of the first selection valves;

[0022] And / or, also includes multiple reagent pools, the multiple reagent pools being connected to one or more first liquid passage ports of the first selection valve;

[0023] And / or, it also includes a waste liquid tank, which is connected to the drive unit.

[0024] In some embodiments, a selection valve is provided, including a plurality of first liquid passage ports of the selection valve, at least one second liquid passage port of the selection valve, and a plurality of third liquid passage ports of the selection valve. The selection valve can be switched to connect one-to-one with the first liquid passage ports of the selection valve and the third liquid passage ports of the selection valve to conduct multiple independent sample channels. The selection valve can also be switched to connect the second liquid passage port of the selection valve with one or more of the third liquid passage ports of the selection valve to conduct at least one reagent channel.

[0025] In some embodiments, the selection valve includes a stator and a rotor, and a first liquid passage port, a second liquid passage port, and a third liquid passage port of the selection valve are disposed on the stator; the rotor is provided with multiple sample channels and at least one reagent channel, the sample channel having a first connection port and a second connection port, and the reagent channel having a first connection port and a second connection port.

[0026] The first connection port of the sample channel is used to switch to connect with the first liquid passage port of the selection valve, and the second connection port of the sample channel is used to switch to connect with the third liquid passage port of the selection valve.

[0027] The first connection port of the reagent channel is used to switch to connect with the second liquid path port of the selector valve, and the second connection port of the reagent channel is used to switch to connect with the third liquid path port of the selector valve.

[0028] In some embodiments, the rotor includes a first rotor and a second rotor, the first rotor and the second rotor being fixedly connected, the first rotor being located between the stator and the second rotor; the first rotor has a first surface facing the stator and a second surface facing away from the stator, the reagent channel is located on the first surface of the first rotor, the sample channel is located on the second surface of the first rotor, and a first connection port and a second connection port of the sample channel extend to the first surface of the first rotor; the stator has a first surface facing away from the first rotor and a second surface facing away from the first rotor, and a first liquid passage port, a second liquid passage port, and a third liquid passage port of the selection valve extend from the first surface of the stator to the second surface of the stator.

[0029] In some embodiments, the first connection port of the sample channel, the second connection port of the sample channel, and the second connection port of the reagent channel are distributed at intervals along a first circumferential trajectory on a first surface of the first rotor. The first connection port of the reagent channel is located at the center of the first circumferential trajectory, and the reagent channel is distributed radially along the first circumferential trajectory. The first liquid passage port and the third liquid passage port of the selection valve are distributed at intervals along a second circumferential trajectory on a second surface of the stator. The second liquid passage port of the selection valve is located at the center of the second circumferential trajectory, and the first circumferential trajectory and the second circumferential trajectory are aligned and coincident in the axial direction of the rotor.

[0030] In some embodiments, the second connection port of the reagent channel is an arc-shaped strip structure distributed along the first circumferential trajectory, and the second connection port of the reagent channel is used to switch to connection with a third liquid passage port of one or more of the selector valves.

[0031] In some embodiments, the selector valve further includes a housing and a drive unit, the stator and the rotor are located inside the housing, the stator is fixedly connected to the housing, the rotor is rotatably connected to the housing, the output end of the drive unit is fixedly connected to the rotor, and the drive unit is used to drive the rotor to rotate relative to the stator.

[0032] According to the liquid circuit system and selection valve of the above embodiment, since the first selection valve includes a plurality of first liquid circuit ports of the first selection valve, at least one second liquid circuit port of the first selection valve, and a plurality of third liquid circuit ports of the first selection valve, and the first selection valve can be switched to connect the first liquid circuit ports of the plurality of first selection valves and the third liquid circuit ports of the plurality of first selection valves one-to-one to conduct multiple independent sample channels, the liquid circuit system can realize the connection of multiple independent sample channels to multiple fluid channels on the chip carrier by switching the first selection valve, so that the multiple fluid channels of the sequencing carrier can be loaded with samples independently, which can greatly improve the sample collection efficiency, shorten the sample collection time, and realize the immediate testing of samples. Attached Figure Description

[0033] Figure 1 is a schematic diagram of the structure of the liquid circuit system in one embodiment;

[0034] Figure 2 is a schematic diagram of the first sampling mode of the liquid circuit system in one embodiment;

[0035] Figure 3 is a schematic diagram of the second sampling mode of the liquid circuit system in one embodiment;

[0036] Figure 4 is a schematic diagram of the third sampling mode of the liquid circuit system in one embodiment;

[0037] Figure 5 is a schematic diagram of the fourth sampling mode of the liquid circuit system in one embodiment;

[0038] Figure 6 is a schematic diagram of the first reagent addition mode of the liquid circuit system in one embodiment;

[0039] Figure 7 is a schematic diagram of the second reagent addition mode of the liquid circuit system in one embodiment;

[0040] Figure 8 is a schematic diagram of the third reagent addition mode of the liquid circuit system in one embodiment;

[0041] Figure 9 is a schematic diagram of the fourth reagent addition mode of the liquid circuit system in one embodiment;

[0042] Figure 10 is an exploded view of the first selection valve in one embodiment;

[0043] Figure 11 is a schematic diagram of the exploded structure of the rotor in one embodiment;

[0044] Figure 12 is a structural perspective view of the first rotor in one embodiment;

[0045] Figure 13 is a top view of the first rotor in one embodiment;

[0046] Figure 14 is a bottom view of the first rotor in one embodiment;

[0047] Figure 15 is a schematic diagram of the first selection valve in one embodiment.

[0048] The reference numerals in the attached figures are as follows: 1-First selection valve, 1a-First liquid path port, 1b-Second liquid path port, 1c-Third liquid path port, 11-Stator, 12-Rotor, 121-First rotor, 122-Second rotor, 13-Sample channel, 13a-First connection port, 13b-Second connection port, 14-Reagent channel, 14a-First connection port, 14b-Second connection port, 15-Housing, 151-Connection hole, 16-Driver; 2-Chip stage, 2a-First liquid path port, 2b-Second liquid path port, 21-Fluid channel; 3-Driver, 31-Unit pump, 3a-First liquid path port, 3b-Second liquid path port; 4-Sample pool; 5-Reagent pool; 6-Second selection valve, 6a-First liquid path port, 6b-Second liquid path port; 7-Waste liquid pool. Detailed Implementation

[0049] The present application will now be described in further detail with reference to the accompanying drawings and specific embodiments. Similar elements in different embodiments are referred to by related similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of the present application. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, certain operations related to the present application are not shown or described in the specification. This is to avoid obscuring the core parts of the present application with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.

[0050] Furthermore, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can be rearranged or adjusted in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and drawings are only for the clear description of a particular embodiment and do not imply a necessary order, unless otherwise stated that a particular order must be followed.

[0051] The serial numbers assigned to components in this document, such as "first" and "second," are used only to distinguish the described objects and have no sequential or technical meaning. The terms "connection" and "linkage" used in this application, unless otherwise specified, include both direct and indirect connections (linkages). Port-to-port connections in this document include the meaning of interconnection, as do other fluid circuit connections.

[0052] In one embodiment, a liquid path system is provided for loading samples and reagents onto sequencing slides. This liquid path system can independently load samples onto multiple flow channels on the sequencing slides, enabling simultaneous sample loading, greatly improving sample loading efficiency, shortening sample loading time, and achieving immediate sample testing upon arrival.

[0053] Please refer to Figures 1 and 10. This liquid path system mainly includes a first selection valve 1, a chip stage 2, and a drive unit 3. The first selection valve 1 is located at the front end of the liquid path of the chip stage 2, and the drive unit 3 is located at the rear end of the liquid path of the chip stage 2. The first selection valve 1 is used to switch between the sample channel and the reagent channel connected to the chip stage 2. The chip stage 2 is used to mount sequencing slides, and the drive unit 3 is used to drive the sample or reagent into the sequencing slide on the chip stage 2.

[0054] The first selection valve 1 has multiple sample channels and at least one reagent channel. The first selection valve 1 can be switched to connect multiple sample channels to the chip stage 2, or to connect only one reagent channel to the chip stage 2. For example, the first selection valve 1 has four sample channels and one reagent channel, and the sequencing slide installed in the chip stage 2 has four liquid channels. The first selection valve 1 can be switched to connect the four sample channels to the four liquid channels in a one-to-one correspondence, enabling independent sample loading for each of the four liquid channels. The first selection valve 1 can also be switched to connect one reagent channel to one, two, three, or all four sample channels to add reagents to the connected liquid channels.

[0055] The first selection valve 1 may include multiple first liquid path ports 1a, at least one second liquid path port 1b, and multiple third liquid path ports 1c. The first liquid path port 1a is a sample input port and is used to connect to the sample pool. The second liquid path port 1b is a reagent input port and is used to connect to the reagent pool. The third liquid path port 1c is an output port and can output samples or reagents. The third liquid path port 1c is connected to the chip stage 2.

[0056] The first selection valve 1 is mainly used to switch between two modes: a sample loading mode and a reagent loading mode. When the first selection valve 1 is switched to the sample loading mode, multiple first liquid path ports 1a are connected one-to-one with multiple third liquid path ports 1c to conduct multiple independent sample channels. These sample channels can independently load samples onto multiple fluid channels 21 on the chip stage 2. When the first selection valve 1 is switched to the reagent loading mode, the second liquid path port 1b is connected to one or more third liquid path ports 1c to conduct at least one reagent channel, adding reagents to at least one of the multiple fluid channels 21 on the chip stage 2. The reagent loading mode can include multiple modes, with different numbers of third liquid path ports 1c connected to the second liquid path port 1b in different modes. For example, when there are four third liquid path ports 1c, the reagent loading mode includes four different modes to connect one, two, three, or four third liquid path ports 1c, achieving reagent loading for different fluid channels 21.

[0057] The chip stage 2 can be used to place and mount one or more sequencing wafers (also called sequencing chips). The chip stage 2 has multiple first liquid access ports 2a and multiple second liquid access ports 2b. Each channel on the sequencing wafer is connected to one second liquid access port 2b, and each channel on the sequencing wafer is connected to one first liquid access port 2a. That is, the multiple first liquid access ports 2a and multiple second liquid access ports 2b on the chip stage 2 limit the upper limit of the channels on which the sequencing wafers can be mounted. During sequencing, the number of channels on the chip stage 2 for mounting the sequencing wafers can be equal to or less than the number of first liquid access ports 2a and second liquid access ports 2b. For example, if there are four first liquid access ports 2a and four second liquid access ports 2b, the number of channels on the chip stage 2 for mounting the sequencing wafers can be four or less. When there are fewer than four channels on the chip stage 2 for mounting the sequencing wafers, the other first liquid access ports 2a and second liquid access ports 2b can remain idle without affecting the use of the first liquid access ports 2a and second liquid access ports 2b connected to channels.

[0058] The first liquid path port 2a and the second liquid path port 2b are located on both sides of the chip stage 2, that is, the first liquid path port 2a and the second liquid path port 2b are located on both sides of the sequencing wafer. The number of the first liquid path ports 2a of the chip stage 2 is the same as the number of the third liquid path ports 1c of the first selection valve 1. The multiple first liquid path ports 2a of the chip stage 2 are connected one-to-one with the multiple third liquid path ports 1c of the first selection valve 1.

[0059] The driver 3 is connected to multiple second liquid channel ports 2b of the chip stage 2. The driver 3 can drive one fluid channel 21 in the sequencing slide to load samples or reagents individually, or it can drive multiple fluid channels 21 in the sequencing slide to load samples or reagents simultaneously.

[0060] The driving component 3 can be a driving pump, which includes multiple unit pumps 31. The number of unit pumps 31 is equal to the number of second liquid passage ports 2b of the chip stage 2. For example, if the number of second liquid passage ports 2b of the chip stage 2 is four, then the driving component 3 includes four unit pumps 31, that is, each unit pump 31 is used to drive the loading of samples and reagents through one fluid channel 21. Each unit pump 31 includes a first liquid passage port 3a and a second liquid passage port 3b. The driving body of the unit pump 31 can be switched to connect to one of the first liquid passage port 3a and the second liquid passage port 3b. The first liquid passage port 3a of each unit pump 31 is connected to one of the second liquid passage ports 2b of the chip stage 2.

[0061] The unit pump 31 has a solenoid valve, which can switch the drive body of the unit pump 31 to be connected to one of the first liquid passage port 3a and the second liquid passage port 3b.

[0062] The drive unit 3 serves as a power source to drive samples, reagents, cleaning solutions, or other media into the liquid path pipeline to achieve gene sequencing, liquid path detection, and liquid path cleaning.

[0063] In other embodiments, multiple driving elements 3 may be provided, each driving element 3 being connected to a second liquid channel port 2b of the chip stage 2, which can also realize the separate driving of multiple fluid channels 21 to load samples or reagents and other media.

[0064] In this embodiment, the liquid path system includes a first selection valve 1 comprising multiple first liquid path ports 1a, at least one second liquid path port 1b, and multiple third liquid path ports 1c. The first selection valve 1 can be switched to connect the multiple first liquid path ports 1a and the multiple third liquid path ports 1c in a one-to-one correspondence, thereby opening multiple independent sample channels. The liquid path system can achieve one-to-one connection between multiple independent sample channels and multiple fluid channels 21 on the chip stage 2 by switching the first selection valve 1. This allows multiple fluid channels 21 of the sequencing wafer to be loaded independently, greatly improving sample loading efficiency, shortening sample loading time, and enabling immediate sample testing upon arrival. Furthermore, since the liquid path system can achieve individual sample loading of the fluid channels 21, it can enrich the rotation of sequencing wafer specifications and cover a wider range of sequencing throughput.

[0065] Please refer to Figure 2. In one embodiment, the liquid circuit system may further include multiple sample pools 4. The multiple sample pools 4 may be set in a container, which has multiple partitioned areas. Each partitioned area forms a sample pool 4. The multiple sample pools 4 may store different samples, or of course, they may store the same samples.

[0066] The number of sample pools 4 is the same as the number of first liquid path ports 1a. Multiple sample pools 4 are connected one-to-one with multiple first liquid path ports 1a of the first selection valve 1. For example, the first selection valve 1 includes four first liquid path ports 1a, and the liquid path system includes four sample pools 4. Each sample pool 4 is connected to one first liquid path port 1a. Multiple sample pools 4, together with multiple independent sample channels in the first selection valve 1, can realize the individual or simultaneous addition of samples to multiple fluid channels 21 on the chip stage 2, and can load different or the same samples.

[0067] Referring to Figure 2, in one embodiment, the liquid circuit system further includes a reagent pool 5 and a second selection valve 6. Multiple reagent pools 5 may be located within a container, which has multiple partitioned areas, each partitioning area forming a reagent pool 5. The second selection valve 6 is disposed between the reagent pool 5 and the first selection valve 1. The second selection valve 6 includes multiple first liquid circuit ports 6a and at least one second liquid circuit port 6b. The number of first liquid circuit ports 6a of the second selection valve 6 is greater than or equal to the number of reagent pools 5. One reagent pool 5 is connected to one first liquid circuit port 6a. Only one second liquid circuit port 6b may be provided, and the second liquid circuit port 6b is connected to the second liquid circuit port 2b of the first selection valve 1.

[0068] The second selection valve 6 can switch one or more first liquid path ports 6a to be connected to the second liquid path port 6b. That is, the second liquid path port 6b can be connected to one first liquid path port 6a to load one reagent, and the second liquid path port 6b can also be connected to multiple first liquid path ports 6a at the same time to load multiple reagents simultaneously.

[0069] In other embodiments, where only one reagent is involved, the second selection valve 6 can be omitted, and the reagent pool 5 can be directly connected to the first selection valve 1.

[0070] Please refer to Figure 2. In one embodiment, the liquid system further includes a waste liquid tank 7. The second liquid port 3b of all unit pumps 31 of the drive unit 3 are respectively connected to the waste liquid tank 7. The waste liquid tank 7 is used to collect test waste liquid or cleaning liquid and other media.

[0071] Referring to Figures 10 to 14, in one embodiment, the first selection valve 1 can be a rotary valve. The first selection valve 1 includes a stator 11 and a rotor 12. The rotor 12 can rotate relative to the stator 11. The first liquid passage port 1a, the second liquid passage port 1b, and the third liquid passage port 1c of the first selection valve 1 are all disposed on the stator 11. The rotor 12 is provided with multiple sample channels 13 and at least one reagent channel 14. After the rotor 12 rotates relative to the stator 11, the sample channels 13 and the reagent channel 14 will rotate relative to the rotor 12 to switch the connection between the first liquid passage port 1a and the third liquid passage port 1c, or the connection between the second liquid passage port 1b and the third liquid passage port 1c.

[0072] Each sample channel 13 has a first connection port 13a and a second connection port 13b, and the reagent channel 14 has a first connection port 14a and a second connection port 14b. The first connection ports 13a, 13b, and 14a are ordinary round port holes, each used to connect to a liquid path port. The second connection port 14b is an elongated port, which can connect to one or more liquid path ports.

[0073] The first connection port 13a of the sample channel 13 is used to switch to connect with the first liquid circuit port 1a of the first selection valve 1, and the second connection port 13b of the sample channel 13 is used to switch to connect with the third liquid circuit port 1c of the first selection valve 1, so that the first selection valve 1 can switch to connect multiple sample channels 13.

[0074] The first connection port 14a of the reagent channel 14 is used to switch to connect to the second liquid passage port 1b of the first selector valve 1, and the second connection port 14b of the reagent channel 14 is used to switch to connect to the third liquid passage port 1c of the first selector valve 1, so that the first selector valve 1 switches to connect to the reagent channel 14.

[0075] This liquid handling system, using four fluid channels 21 as an example, illustrates the switchable modes. This system can switch to four sample loading modes and four reagent loading modes to achieve high-throughput loading of samples and reagents across multiple channels, as detailed below:

[0076] I. Sampling Mode

[0077] In different sample dispensing modes, the first selection valve 1 switches to the same mode, the difference being that the drive unit 3 switches to different modes. Specifically, when the first selection valve 1 switches to the first connection port 13a of the four sample channels 13, they are connected one-to-one with the four first liquid passage ports 1a of the first selection valve 1, and the second connection port 13b of the sample channels 13 are connected one-to-one with the four third liquid passage ports 1c of the first selection valve 1. That is, when the first selection valve 1 switches to the four sample channels 13, they are connected one-to-one with the four fluid channels 21.

[0078] (1) First sample loading mode: Please refer to Figure 2. The drive body of one unit pump 31 is connected to the first liquid channel port 3a, and the drive bodies of the other three unit pumps 31 are connected to the second liquid channel port 3b. That is, one fluid channel 21 on the chip stage 2 is connected to one unit pump 31, and the other three fluid channels 21 are not connected to the unit pump 31. At this time, the liquid channel system can load samples onto the connected fluid channel 21.

[0079] (2) Second sample loading mode: Please refer to Figure 3. The driving bodies of the two unit pumps 31 are connected to the first liquid channel port 3a, and the driving bodies of the other two unit pumps 31 are connected to the second liquid channel port 3b. That is, the two fluid channels 21 on the chip stage 2 are connected to one unit pump 31, and the other two fluid channels 21 are not connected to the unit pumps 31. At this time, the liquid system can load samples onto the two connected fluid channels 21 respectively.

[0080] (3) Third sample loading mode: Please refer to Figure 4. The drive body of the three unit pumps 31 is connected to the first liquid channel port 3a, and the drive body of the other unit pump 31 is connected to the second liquid channel port 3b. That is, the three fluid channels 21 on the chip stage 2 are connected to one unit pump 31, and the other fluid channel 21 is not connected to the unit pump 31. At this time, the liquid channel system can load samples onto the three connected fluid channels 21 respectively.

[0081] (4) Fourth sample loading mode: Please refer to Figure 5. The drive body of the four unit pumps 31 is connected to the first liquid channel port 3a; that is, the four fluid channels 21 on the chip stage 2 are connected to one unit pump 31. At this time, the liquid system can load samples onto the four connected fluid channels 21 respectively.

[0082] II. Reagent Addition Mode

[0083] In different reagent addition modes, the first selection valve 1 switches to different modes, and the drive unit 3 switches to different modes.

[0084] (1) First reagent addition mode: Please refer to Figure 6. The first selection valve 1 switches to the second connection port 14b of the four reagent channels 14 and connects to one of the third liquid passage ports 1c of the first selection valve 1. That is, the first selection valve 1 switches to the reagent channel 14 and connects to one fluid channel 21 respectively. The drive body of one unit pump 31 is connected to the first liquid passage port 3a, and the drive bodies of the other three unit pumps 31 are connected to the second liquid passage port 3b. That is, one fluid channel 21 on the chip stage 2 is connected to one unit pump 31, and the other three fluid channels 21 are not connected to the unit pump 31. At this time, the liquid system can load reagents onto the connected fluid channel 21.

[0085] (2) Second reagent addition mode: Please refer to Figure 7. The first selection valve 1 switches to the second connection port 14b of the four reagent channels 14 and connects to the two third liquid circuit ports 1c of the first selection valve 1. That is, the first selection valve 1 switches to the reagent channel 14 and connects to the two fluid channels 21 respectively. The drive body of the two unit pumps 31 is connected to the first liquid circuit port 3a, and the drive body of the other two unit pumps 31 is connected to the second liquid circuit port 3b. That is, the two fluid channels 21 on the chip stage 2 are connected to one unit pump 31, and the other two fluid channels 21 are not connected to the unit pumps 31. At this time, the liquid circuit system can load reagents onto the two connected fluid channels 21 respectively.

[0086] (3) Third reagent addition mode: Please refer to Figure 8. The first selection valve 1 switches to the second connection port 14b of the four reagent channels 14 and connects to the three third liquid circuit ports 1c of the first selection valve 1. That is, the first selection valve 1 switches to the reagent channel 14 and connects to the three fluid channels 21 respectively. The drive body of the three unit pumps 31 is connected to the first liquid circuit port 3a, and the drive body of the other unit pump 31 is connected to the second liquid circuit port 3b. That is, the three fluid channels 21 on the chip stage 2 are connected to one unit pump 31, and the other fluid channel 21 is not connected to the unit pump 31. At this time, the liquid circuit system can load reagents onto the three connected fluid channels 21 respectively.

[0087] (4) Fourth reagent addition mode: Please refer to Figure 9. The first selection valve 1 switches to the second connection port 14b of the four reagent channels 14 and connects to the four third liquid circuit ports 1c of the first selection valve 1. That is, the first selection valve 1 switches to the reagent channel 14 and connects to the four fluid channels 21 respectively. The drive body of the four unit pumps 31 is connected to the first liquid circuit port 3a. That is, the four fluid channels 21 on the chip stage 2 are connected to one unit pump 31. At this time, the liquid circuit system can load reagents to the four connected fluid channels 21 respectively.

[0088] In one embodiment, the rotor 12 includes a first rotor 121 and a second rotor 122, which are stacked and fixedly connected along the axial direction, with the first rotor 121 located between the stator 11 and the second rotor 122.

[0089] The stator 11 has a first surface facing away from the first rotor 121 and a second surface facing the first rotor 121. A first liquid passage port 1a, a second liquid passage port 1b, and a third liquid passage port 1c extend from the first surface of the stator 11 to the second surface of the stator 11. That is, the stator 11 has multiple through holes along the axial direction, forming the first liquid passage port 1a, the second liquid passage port 1b, and the third liquid passage port 1c. The multiple first liquid passage ports 1a and the multiple third liquid passage ports 1c are located on a second circumferential trajectory on the second surface of the stator 11, and also on a circumferential trajectory on the first surface of the stator 11. The second liquid passage port 1b is located at the center of the second circumferential trajectory, which is located on the rotation center line of the rotor 12.

[0090] The first rotor 121 has a first surface facing the stator 11 and a second surface facing away from the stator. The second surface of the first rotor 121 is attached and fixed to the second rotor 122. The first surface of the first rotor 121 has a reagent channel 14, as well as a first connection port 14a and a second connection port 14b having the reagent channel 14. The first surface of the first rotor 121 has a groove, which, together with the second surface of the stator 11, forms the reagent channel 14. The second surface of the first rotor 121 has multiple sample channels 13, with the first connection port 13a and the second connection port 13b of the multiple sample channels 13 extending from the second surface of the first rotor 121 to the first surface. The second surface of the first rotor 121 has multiple grooves, which, together with the surface of the second rotor 122 facing the first rotor 121, form multiple sample channels 13, which are spaced apart from each other and independent.

[0091] On the first surface of the first rotor 121, a plurality of first connection ports 13a, a plurality of second connection ports 13b, and a second connection port 14b are located on a first circumferential track, with the first connection port 14a located at the center of the first circumferential track. The first and second circumferential tracks are aligned and coincident on the axis of the rotor 12, that is, the first and second circumferential tracks have the same diameter, and their centers are both located on the rotation center line of the rotor 12. This allows the first rotor 121 to switch connections with the plurality of first connection ports 13a, the plurality of second connection ports 13b, or the second connection port 14b on the first surface of the first rotor 121 to the plurality of first liquid passage ports 1a and the plurality of third liquid passage ports 1c on the second surface of the stator 11 when the first rotor 121 rotates relative to the stator 11.

[0092] By configuring the rotor 12 as a combination of the first rotor 121 and the second rotor 122, the reagent channel 14 can be disposed on the first surface of the rotor, and multiple sample channels 13 can be disposed on the second surface of the rotor 12. This allows the reagent channel 14 and the multiple sample channels 13 to be staggered, and they can be closer to the rotation centerline of the rotor 12, thereby reducing the diameter of the rotor 12 and miniaturizing the first selection valve 1. Furthermore, both the sample channels 13 and the reagent channels 14 on the rotor 12 form channels without dead angles, and during the rotation of the rotor 12, only the connection port of the sample channel 13 connects with the liquid port of the stator 11, which reduces the intersection area between the sample channels 13 and the reagent channels 14 and reduces reagent contamination.

[0093] The rotor 12 is divided into two parts, which facilitates the processing of the sample channel 13.

[0094] In one embodiment, the second connection port 14b of the reagent channel 14 is an arc-shaped strip structure extending along the first circumferential trajectory, allowing the second connection port 14b to be switched to connect with one or more third liquid passage ports 1c. The reagent channels 14 are radially linearly distributed along the first circumferential trajectory, ultimately forming a sickle-shaped structure. In other embodiments, the reagent channels 14 may also include multiple channels, with multiple reagent channels 14 sharing a first connection port 14a and each having its own second connection port 14b located on the first circumferential trajectory. This structure can also enable the reagent channels 14 to connect with one or more fluid channels 21.

[0095] Referring to Figure 15, in one embodiment, the first selector valve 1 further includes a housing 15 and a drive member 16. The housing 15 has a receiving cavity, a stator 11 is fixedly installed in the receiving cavity of the housing 15, and a rotor 12 is rotatably installed in the receiving cavity of the housing 15. The second surface of the stator 11 and the first surface of the first rotor 121 are in contact. The housing 15 is provided with a plurality of connection holes 151, which are respectively provided with a plurality of first liquid passage ports 1a, second liquid passage ports 1b and third liquid passage ports 1c. The plurality of first liquid passage ports 1a, second liquid passage ports 1b and third liquid passage ports 1c are connected to other components through the corresponding connection holes 151.

[0096] The drive unit 16 can be installed inside the housing 15 or outside the housing 15. The drive unit 16 can be a drive motor. The drive unit 16 has an output end, which is fixedly connected to the rotor 12. The drive unit 16 is used to drive the rotor 12 to rotate relative to the stator 11, so as to realize the switching of different modes of the first selection valve 1.

[0097] In one embodiment, a selector valve is provided. This selector valve is the first selector valve 1 in any of the above embodiments. This selector valve is a rotary valve that switches between different modes by rotation.

[0098] The above examples illustrate this application only to aid understanding and are not intended to limit its scope. Those skilled in the art to which this application pertains can make various simple deductions, modifications, or substitutions based on the ideas presented.

Claims

1. A fluid circuit system, characterized in that, include: The first selection valve includes a plurality of first liquid passage ports of the first selection valve, at least one second liquid passage port of the first selection valve, and a plurality of third liquid passage ports of the first selection valve. The first selection valve can be switched to connect the plurality of first liquid passage ports of the first selection valve to the plurality of third liquid passage ports of the first selection valve in a one-to-one correspondence to conduct multiple independent sample channels. The first selection valve can also be switched to connect the second liquid passage port of the first selection valve to one or more of the plurality of third liquid passage ports of the first selection valve to conduct at least one reagent channel. A chip stage, the chip stage including a plurality of first liquid passage ports and a plurality of second liquid passage ports of the chip stage, the chip stage being used to place a sequencing slide, the sequencing slide having one or more fluid channels, one end of the fluid channel being connected to a first liquid passage port of the chip stage, and the other end of the fluid channel being connected to a second liquid passage port of the chip stage; A driving element is connected to the second liquid channel port of the plurality of chip stages. The driving element is used to drive the sample or reagent through the first selection valve into the sequencing wafer on the chip stage.

2. The fluid circuit system as described in claim 1, characterized in that, The first selection valve includes a stator and a rotor. The first liquid passage port, the second liquid passage port, and the third liquid passage port of the first selection valve are disposed on the stator. The rotor is provided with multiple sample channels and at least one reagent channel. The sample channel has a first connection port and a second connection port, and the reagent channel has a first connection port and a second connection port. The first connection port of the sample channel is used to switch to connect with the first liquid circuit port of the first selection valve, and the second connection port of the sample channel is used to switch to connect with the third liquid circuit port of the first selection valve. The first connection port of the reagent channel is used to switch to connect to the second liquid path port of the first selector valve, and the second connection port of the reagent channel is used to switch to connect to the third liquid path port of the first selector valve.

3. The fluid circuit system as described in claim 2, characterized in that, The rotor includes a first rotor and a second rotor, which are fixedly connected. The first rotor is located between the stator and the second rotor. The first rotor has a first surface facing the stator and a second surface facing away from the stator. The reagent channel is located on the first surface of the first rotor, and the sample channel is located on the second surface of the first rotor. The first connection port and the second connection port of the sample channel extend to the first surface of the first rotor. The stator has a first surface facing away from the first rotor and a second surface facing away from the first rotor. The first liquid passage port, the second liquid passage port, and the third liquid passage port of the first selection valve extend from the first surface of the stator to the second surface of the stator.

4. The fluid circuit system as described in claim 3, characterized in that, The first connection port of the sample channel, the second connection port of the sample channel, and the second connection port of the reagent channel are distributed at intervals along the first circumferential trajectory of the first surface of the first rotor. The first connection port of the reagent channel is located at the center of the first circumferential trajectory, and the reagent channel is distributed radially along the first circumferential trajectory. The first liquid passage port and the third liquid passage port of the first selection valve are distributed at intervals along the second circumferential trajectory of the second surface of the stator. The second liquid passage port of the first selection valve is located at the center of the second circumferential trajectory, and the first circumferential trajectory and the second circumferential trajectory are aligned and coincident in the axial direction of the rotor.

5. The hydraulic system as described in claim 4, characterized in that, The second connection port of the reagent channel is an arc-shaped strip structure distributed along the first circumferential trajectory. The second connection port of the reagent channel is used to switch to connect to the third liquid path port of one or more of the first selection valves.

6. The fluid circuit system according to any one of claims 2 to 5, characterized in that, The first selector valve further includes a housing and a drive unit. The stator and the rotor are located inside the housing. The stator is fixedly connected to the housing, and the rotor is rotatably connected to the housing. The output end of the drive unit is fixedly connected to the rotor, and the drive unit is used to drive the rotor to rotate relative to the stator.

7. The fluid circuit system as described in claim 1, characterized in that, It also includes a second selector valve, which includes a plurality of first liquid passage ports of the second selector valve and at least one second liquid passage port of the second selector valve, wherein the second liquid passage port of the second selector valve is connected to the second liquid passage port of the first selector valve; the second selector valve is used to switch to one or more connections between the first liquid passage ports of the second selector valve and the second liquid passage ports of the second selector valve.

8. The fluid circuit system as described in claim 1, characterized in that, The drive unit includes multiple unit pumps, each unit pump including a first liquid passage port and a second liquid passage port, and the first liquid passage port of each unit pump is connected to the second liquid passage port of the chip stage.

9. The fluid circuit system as described in claim 1, characterized in that, It also includes multiple sample pools, which are connected to one or more first liquid passage ports of the first selection valve; And / or, also includes multiple reagent pools, the multiple reagent pools being connected to one or more first liquid passage ports of the first selection valve; And / or, it also includes a waste liquid tank, which is connected to the drive unit.

10. A selector valve, characterized in that, The device includes a plurality of first liquid passage ports of the selection valve, at least one second liquid passage port of the selection valve, and a plurality of third liquid passage ports of the selection valve. The selection valve can be switched to connect one-to-one with the first liquid passage ports of the selection valve and the third liquid passage ports of the selection valve to conduct multiple independent sample channels. The selection valve can also be switched to connect the second liquid passage port of the selection valve with one or more of the third liquid passage ports of the selection valve to conduct at least one reagent channel.

11. The selector valve as claimed in claim 10, characterized in that, The selection valve includes a stator and a rotor. The first liquid passage port, the second liquid passage port, and the third liquid passage port of the selection valve are disposed on the stator. The rotor is provided with multiple sample channels and at least one reagent channel. The sample channel has a first connection port and a second connection port, and the reagent channel has a first connection port and a second connection port. The first connection port of the sample channel is used to switch to connect with the first liquid passage port of the selection valve, and the second connection port of the sample channel is used to switch to connect with the third liquid passage port of the selection valve. The first connection port of the reagent channel is used to switch to connect with the second liquid path port of the selector valve, and the second connection port of the reagent channel is used to switch to connect with the third liquid path port of the selector valve.

12. The selector valve as claimed in claim 11, characterized in that, The rotor includes a first rotor and a second rotor, which are fixedly connected. The first rotor is located between the stator and the second rotor. The first rotor has a first surface facing the stator and a second surface facing away from the stator. The reagent channel is located on the first surface of the first rotor, and the sample channel is located on the second surface of the first rotor. The first connection port and the second connection port of the sample channel extend to the first surface of the first rotor. The stator has a first surface facing away from the first rotor and a second surface facing away from the first rotor. The first liquid passage port, the second liquid passage port, and the third liquid passage port of the selection valve extend from the first surface of the stator to the second surface of the stator.

13. The selector valve as claimed in claim 12, characterized in that, The first and second connection ports of the sample channel and the second connection port of the reagent channel are distributed at intervals along a first circumferential trajectory on the first surface of the first rotor. The first connection port of the reagent channel is located at the center of the first circumferential trajectory, and the reagent channel is distributed radially along the first circumferential trajectory. The first and third liquid passage ports of the selection valve are distributed at intervals along a second circumferential trajectory on the second surface of the stator. The second liquid passage port of the selection valve is located at the center of the second circumferential trajectory. The first and second circumferential trajectories are aligned and coincident in the axial direction of the rotor.

14. The selector valve as claimed in claim 13, characterized in that, The second connection port of the reagent channel is an arc-shaped strip structure distributed along the first circumferential trajectory. The second connection port of the reagent channel is used to switch to connect to the third liquid path port of one or more of the selection valves.

15. The selector valve according to any one of claims 11 to 14, characterized in that, The selector valve further includes a housing and a drive unit. The stator and the rotor are located inside the housing. The stator is fixedly connected to the housing, and the rotor is rotatably connected to the housing. The output end of the drive unit is fixedly connected to the rotor, and the drive unit is used to drive the rotor to rotate relative to the stator.

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