Curled hyaluronate sodium wool fiber and preparation method therefor

Abstract

The present invention relates to a curled hyaluronate sodium wool fiber and a preparation method therefor, and more specifically, to a curled hyaluronate sodium wool fiber comprising active ingredients for skin improvement and a preparation method therefor. The preparation method for the curled hyaluronate sodium wool fiber comprises the steps of: (a) preparing a hyaluronate sodium paste with the moisture content adjusted to 50-95 wt% by adding and mixing a solvent into hyaluronate sodium having a weight-average molecular weight of 50-3,000kDa and active ingredients for skin improvement; (b) extruding and spinning the moisture content-adjusted hyaluronate sodium paste through a nozzle at 15-70°C; and (c) forming a curled wool fiber by blow-drying the extruded spun material at 30-70°C. The curled hyaluronate sodium wool fiber according to the present invention can not only be easily produced into a nonwoven fabric due to the rough and wide surface but also be widely utilized in food products, cosmetics, medical devices, and pharmaceuticals due to inclusion therein of heat-sensitive active ingredients for skin improvement, such as vitamin, stem cell culture fluid, and exosomes.

Description

Curly hyaluronic acid wool fiber and method for manufacturing the same

[0001] The present invention relates to a curled hyaluronic acid wool fiber and a method for manufacturing the same, and more specifically, to a curled hyaluronic acid wool fiber containing an active ingredient for skin improvement and a method for manufacturing the same.

[0002] Recently, the elderly population has been increasing and perceptions of cosmetic surgery are changing. Consequently, interest in the development of products aimed at improving quality of life, particularly medical devices, pharmaceuticals, and cosmetics utilizing hyaluronic acid, is rapidly growing.

[0003] Hyaluronic acid, or hyaluronic acid salts, are biocompatible substances widely distributed in the human body's connective tissue, epithelial, and nervous tissues. With excellent water retention and viscoelasticity, and proven effects in skin regeneration, moisturization, elasticity maintenance, and wrinkle improvement, its fields of application are rapidly expanding.

[0004] Most finished hyaluronic acid products currently on the market are in liquid form, and due to their high water content, the use of sterile facilities or preservatives is essential. As most patents related to these liquid products have expired, fierce price competition is taking place in the market.

[0005] Accordingly, the inventors have secured a method for manufacturing solidified hyaluronic acid in various forms, such as films, fibers, and nonwoven fabrics, which have increased resistance to microorganisms and other pathogens unlike existing liquid formulations, by processing hyaluronic acid under various conditions, and are proceeding with the development of various products utilizing this method (Aesthetic Plastic Surgery, 48:221~227, 2024, J. of The Korean Academy of Pediatric Dentistry, 49(3): 300-309, 2022, J. of Dental Sciences, 17(4): 1595-1603, 2022. Oral Disease, accepted 2023.).

[0006] Korean Registered Patent No. 1709608 disclosed a method for manufacturing hyaluronic acid fibers by melt spinning, comprising: (a) a step of controlling the moisture content of a hyaluronic acid salt having a weight-average molecular weight of 500 to 3,000 kDa to 5 to 20%; (b) a step of placing the hyaluronic acid salt with controlled moisture content into a melt spinning device, heating it to 150 to 200°C, and then high-pressure spinning to produce hyaluronic acid fibers; and (c) a step of immersing the hyaluronic acid fibers in an aqueous ethanol solution to harden the surface, and Korean Registered Patent No. 2426699 disclosed a method comprising: (a) a step of manufacturing a hyaluronic acid paste by controlling the moisture content of a hyaluronic acid salt having a weight-average molecular weight of 100 to 3,000 kDa to 50 to 95 weight%; (b) a step of melting a hyaluronic acid paste with controlled moisture content at 15°C to 100°C and then extruding it through a nozzle; and (c) a step of drying the extruded spinning fluid to form a fiber, thereby disclosing a method for manufacturing a hyaluronic acid fiber.

[0007] However, these are hyaluronic acid fibers in the form of smooth filaments, and when using them to manufacture nonwoven fabric, there were difficulties in forming the nonwoven fabric because the entanglement force was weak during the needle punching process after web formation.

[0008] To solve this problem, the inventor disclosed in Korean Registered Patent No. 2475485 a method of manufacturing a nonwoven fabric by drying an extruded hyaluronic acid spinning fluid at a high temperature of 100 to 150°C to form a fiber with a rough surface and using the same.

[0009] Meanwhile, Korean Registered Patent No. 2475485 disclosed the mixing of active ingredients for skin improvement, such as collagen, chitosan, and alginic acid other than hyaluronic acid, into fibers during web formation; however, ingredients such as vitamins and stem cell culture media, which cannot be manufactured in fiber form, could not be applied, and there was a problem in that the active ingredients were destroyed during the high-temperature drying stage of the spinning fluid when these active ingredients were mixed into the hyaluronic acid paste manufacturing stage.

[0010] Accordingly, the inventors of the present invention, having made efforts to solve the above problem, confirmed that when a hyaluronic acid paste containing an active ingredient for skin improvement is prepared and then air-dried at room temperature to 70°C instead of high-temperature drying at 100 to 150°C, a wool fiber with a rough surface and curl can be manufactured more simply than the existing method without destroying the active ingredient for skin improvement, and thus completed the present invention.

[0011] The objective of the present invention is to provide a curled wool fiber containing an active ingredient for skin improvement and a method for manufacturing the same.

[0012] Another objective of the present invention is to provide a nonwoven fabric, a wound dressing, a shaping filler, and a cosmetic comprising curled wool fibers containing an active ingredient for skin improvement.

[0013] To achieve the above objective, the present invention provides a method for manufacturing a curled hyaluronic acid wool fiber comprising: (a) a step of adding a solvent to a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for skin improvement, and mixing and kneading to produce a hyaluronic acid paste with a moisture content controlled to 50 to 95 weight%; (b) a step of extruding the hyaluronic acid paste with controlled moisture content through a nozzle at 15 to 70°C; and (c) a step of blow-drying the extruded spinning fluid at 30 to 70°C to form a curled wool fiber.

[0014] In the present invention, the active ingredient for skin improvement is characterized by being one or more selected from the group consisting of (a) moisturizing and barrier strengthening ingredients, (b) antioxidant ingredients, (c) skin regeneration ingredients, (d) skin soothing and anti-inflammatory ingredients, and (e) whitening ingredients.

[0015] In the present invention, the active ingredient for skin improvement is characterized by being selected from the group consisting of vitamins, peptides, stem cell culture medium, and exosomes.

[0016] In the present invention, the skin-improving active ingredient is characterized in that it is present in an amount of 0.1 to 20 parts by weight per 100 parts by weight of hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa.

[0017] The present invention also provides a curled hyaluronic acid wool fiber comprising a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for improving skin.

[0018] The present invention also provides a nonwoven fabric comprising a curled hyaluronic acid wool fiber having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for improving skin.

[0019] The present invention also provides a wound dressing comprising a hyaluronic acid wool fiber with curls, comprising a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for improving skin.

[0020] The present invention also provides a shaping filler comprising a hyaluronic acid wool fiber with curls, comprising a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for improving skin.

[0021] The present invention also provides a cosmetic product comprising a hyaluronic acid wool fiber with curls, comprising a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for improving skin.

[0022] The curled hyaluronic acid wool fiber of the present invention has a rough and wide surface, making it easy to manufacture into a nonwoven fabric, and also contains active ingredients for improving skin that is sensitive to heat, such as vitamins, stem cell culture medium, and exosomes.

[0023] FIG. 1 is a vial-shaped product containing curled hyaluronic acid wool fibers manufactured according to the present invention.

[0024] FIG. 2 is a specification sheet of a stem cell culture medium used in one embodiment of the present invention.

[0025] Figure 3 is a photograph of a nonwoven fabric comprising curled hyaluronic acid wool fibers manufactured according to the present invention.

[0026] FIG. 4 is a general photograph and a micrograph of hyaluronic acid fibers prepared according to an embodiment and a comparative example of the present invention (A: curled hyaluronic acid wool fiber, B: filament-type hyaluronic acid fiber, C: curled hyaluronic acid wool fiber that is not dried by blow-drying at low temperature and has some aggregated crystals).

[0027] In the present invention, we intended to confirm that when a hyaluronic acid paste containing an active ingredient for skin improvement is prepared and then extruded and air-dried at 30 to 70°C instead of high-temperature drying at 100 to 150°C, a wool fiber with a rough surface and curl can be manufactured more simply than the existing method without destroying the active ingredient for skin improvement.

[0028] In the present invention, a solvent was added to hyaluronic acid salt and an active ingredient for skin improvement and mixed to form a paste with a moisture content of 50 to 95% by weight. Then, the paste was extruded, and the extruded spinning fluid was air-dried at 30 to 70°C to produce a curled wool fiber. Next, it was confirmed that the production of a nonwoven fabric was facilitated using the produced curled wool fiber, and it was confirmed that the active ingredient for skin improvement contained in the produced nonwoven fabric was not destroyed and maintained its effect.

[0029] Accordingly, in one aspect, the present invention relates to a method for manufacturing a curled hyaluronic acid wool fiber comprising: (a) a step of adding a solvent to a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for skin improvement, and mixing and kneading to produce a hyaluronic acid paste with a moisture content controlled to 50 to 95 weight%; (b) a step of extruding the hyaluronic acid paste with controlled moisture content through a nozzle at 15 to 70°C; and (c) a step of blow-drying the extruded spinning fluid at 30 to 70°C to form a curled wool fiber.

[0030] In the present invention, the hyaluronic acid salt is a salt formed by binding a salt to hyaluronic acid, and examples include sodium hyaluronate, calcium hyaluronate, potassium hyaluronate, etc., but are not limited thereto.

[0031] In the present invention, the molecular weight of the hyaluronic acid salt is preferably 50 to 3,000 kDa. In addition, the hyaluronic acid salt paste is characterized by having a water content of 50 to 95 weight% of the hyaluronic acid salt.

[0032] The above hyaluronic acid paste can be prepared by adding a solvent to the hyaluronic acid, wherein the solvent is water or an aqueous solution of ethanol, and it is preferable to mix until the solvent is uniformly mixed.

[0033] The above-mentioned active ingredients for skin improvement are added during the preparation of hyaluronic acid paste to enhance the skin improvement effect, and examples include (a) moisturizing and barrier-strengthening ingredients, (b) antioxidant ingredients, (c) skin regeneration ingredients, (d) skin soothing and anti-inflammatory ingredients, (e) whitening ingredients, etc.

[0034] Examples of moisturizing and barrier-strengthening ingredients include glycerin, betaine, panthenol, squalane, ceramide, cholesterol, and fatty acids; examples of antioxidant ingredients include vitamin C, tocopherol, niacinamide, coenzyme Q10, alpha-lipoic acid, polyphenols, and flavonoids; examples of skin regeneration ingredients known to promote cell regeneration include retinol, retinyl palmitate, peptides, copper peptides, adenosine, beta-glucan, and madecassoside; examples of soothing and anti-inflammatory ingredients include allantoin, panthenol, Centella asiatica extract, chamomile extract, aloe vera leaf extract, green tea extract, licorice extract, zinc oxide, bisabolol, madecassoside, beta-sitosterol, and ceramide; and examples of whitening ingredients include niacinamide, arbutin, kojic acid, tranexamic acid, glutathione, and vitamin C derivatives. Examples of active ingredients include alpha-abutin, but are not limited thereto. Other active ingredients may include collagen, elastin, astaxanthin, propolis, snail mucin, sericin, stem cell culture medium, exosomes, etc.

[0035] It is preferable that the above-mentioned active ingredient for skin improvement be included in an amount of 0.1 to 20 parts by weight per 100 parts by weight of hyaluronic acid having a weight-average molecular weight of 50 to 3,000 kDa. If the amount is less than 0.1 parts by weight, the effect of the skin-improving active ingredient is negligible, and if the amount exceeds 20 parts by weight, it may be difficult to manufacture hyaluronic acid wool fibers or physical properties such as tensile strength may be reduced.

[0036] In order to smoothly carry out the above mass spinning process, the moisture content of the hyaluronic acid paste may be 50 to 95 weight percent, preferably 88 to 92 weight percent. If the moisture content is less than 50 weight percent, high pressure is required during spinning, and if the moisture content exceeds 95 weight percent, there is a problem that the thread breaks during spinning because it is in a liquid form rather than a paste.

[0037] In the present invention, a hyaluronic acid paste with a moisture content adjusted to 50 to 95 weight percent is placed in a spinning device, melted at 15 to 70°C, and then extruded through a nozzle to produce a spinning fluid. At this time, if the temperature is below 15°C, the hyaluronic acid paste does not melt and therefore cannot be extruded, and if the temperature exceeds 70°C, the active ingredient for skin improvement, which has poor thermal stability, may be destroyed.

[0038] In the present invention, fibers are finally formed by drying an extruded spinning fluid, and the invention is characterized by manufacturing wool fibers with curls without destroying active ingredients for skin improvement by blow-drying at 30 to 70°C. If the temperature of the blow-drying is less than 30°C, it is not completely dried, so some crystals are formed, or the surface of the fibers is smooth because curls are not imparted, so the entanglement force is weak during the needle punching process after web formation, making it difficult to form a nonwoven fabric, and if it exceeds 70°C, there is a problem that active ingredients for skin improvement are destroyed.

[0039] For reference, Korean registered patent No. 10-2475485 performed general drying at a high temperature of 100~150℃ to produce rough-surfaced fibers for the manufacture of nonwoven fabric.

[0040]

[0041] In another aspect, the present invention relates to a curled hyaluronic acid wool fiber comprising a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for improving skin.

[0042] The above-mentioned curled hyaluronic acid wool fiber has a moisture content of 10 to 20% and a bulk density of 0.3 to 0.35 g / ml.

[0043] The above bulk density indicates the degree of surface roughness and curl imparted to the fibers, and is measured by the following method with reference to ASTM D7481 or USP 616.

[0044] (a) Prepare 1 g of curled hyaluronic acid wool fiber with a length of 1 mm and a diameter of 0.05 mm.

[0045] (b) After adding this to a 20 mL graduated cylinder, measure the final volume by tapping 10 to 20 times or more with a plastic stick.

[0046] (c) Calculate Bulk Density using the following formula

[0047] Bulk density = Mass / Volume (g / ml)

[0048]

[0049] The curled hyaluronic acid wool fiber of the present invention has a rough surface, which increases its surface area and thus increases its solubility in water compared to conventional filament-shaped hyaluronic acid fibers, resulting in excellent usability. Furthermore, it is characterized by containing an active ingredient for skin improvement with low thermal stability.

[0050] As shown in FIG. 1, the curled hyaluronic acid wool fiber according to the present invention is stored in a vial and immediately dissolved by adding purified water, saline solution, or sterile water for injection just before use, and can be used as a cosmetic, skin booster, dermal filler, or injectable drug.

[0051] The curled hyaluronic acid wool fiber of the present invention has excellent utility for secondary processing into nonwoven fabric.

[0052] Accordingly, the present invention relates to a nonwoven fabric comprising a curled hyaluronic acid wool fiber having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for improving skin.

[0053] The nonwoven fabric comprising the above-mentioned curled hyaluronic acid wool fiber can be manufactured by a method comprising the steps of: (a) cutting the curled hyaluronic acid wool fiber; (b) forming a web with the cut curled hyaluronic acid wool fiber; and (c) needle punching the formed hyaluronic acid web.

[0054] To form a web for manufacturing a nonwoven fabric, the manufactured curled hyaluronic acid wool fibers are cut to a desired length, and the cut curled hyaluronic acid wool fibers are dispersed by air pressure and then extruded by air pressure to form the web through a carding and air-laid process.

[0055] The needle punching step of the present invention is a step of punching the formed flat sheet web with a needle. Specifically, the needle punching step can manufacture a nonwoven fabric by repeatedly moving a needle plate with a needle attached up and down while passing it through a Needle Loom, thereby combining a portion of a two-dimensional random fiber arrangement into a three-dimensional random structure.

[0056] The nonwoven fabric made of the curled hyaluronic acid wool fiber of the present invention can be used for purposes such as mask packs for external skin preparations, wound dressings, biomaterials for tissue repair for medical devices, and anti-adhesion dressings.

[0057] The present invention will be described in more detail below through examples. These examples are solely for illustrating the present invention, and it will be obvious to those skilled in the art that the scope of the present invention is not to be interpreted as being limited by these examples.

[0058]

[0059] Example 1: Preparation of a hyaluronic acid paste containing stem cell culture medium components

[0060] Sodium hyaluronate with a molecular weight of 150 kDa (Hi-Aqua TM Water was added to 100g of (Jinwoo Bio Co., Ltd.) and 1g of stem cell culture medium (Jungjin Bioscience, JJ-UCM, see Fig. 2) and mixed to prepare a hyaluronic acid-based paste with a moisture content of 50–95%. The prepared hyaluronic acid paste was refrigerated at 4°C for 12 hours to induce moisture equilibrium.

[0061]

[0062] Example 2: Preparation of curled hyaluronic acid wool fibers containing stem cell culture medium components

[0063] The hyaluronic acid paste prepared in Example 1 was placed in the storage section of a spinning device, and the paste was pressurized at room temperature (15–25°C) and extruded through a nozzle, and air-dried at 50–60°C to produce a hyaluronic acid wool fiber with curls (Fig. 4A).

[0064]

[0065] Comparative Example 1: Preparation of hyaluronic acid fiber containing stem cell culture medium components (filament type)

[0066] The hyaluronic acid paste prepared in Example 1 was placed in the storage section of a spinning device, and the paste was pressurized at room temperature (15–25°C) and extruded through a nozzle, and then dried at room temperature (15–25°C) to produce hyaluronic acid fibers (Fig. 4B).

[0067]

[0068] Comparative Example 2: Preparation of hyaluronic acid fibers containing stem cell culture medium components (high-temperature static drying)

[0069] The hyaluronic acid paste prepared in Example 1 was placed in the storage section of a spinning device, melted at 15 to 100°C, then pressurized and extruded through a nozzle, and dried at 120°C to produce hyaluronic acid fibers.

[0070]

[0071] Comparative Example 3: Preparation of hyaluronic acid fibers containing stem cell culture medium components (crystal precipitation)

[0072] The hyaluronic acid paste prepared in Example 1 was placed in the storage section of a spinning device, and the paste was pressurized at room temperature (15–25°C), spun through a nozzle, and air-dried at room temperature (15–25°C) to produce hyaluronic acid wool fibers (Fig. 4C).

[0073]

[0074] Comparative Example 4: Preparation of curled hyaluronic acid wool fibers containing stem cell culture medium components (high-temperature blow-dried)

[0075] The hyaluronic acid paste prepared in Example 1 was placed in the storage section of a spinning device, and the paste was pressurized at room temperature (15~25℃), spun through a nozzle, and blow-dried at 80℃ to produce a hyaluronic acid wool fiber with curls.

[0076]

[0077] Example 3: Preparation of a nonwoven fabric comprising curled hyaluronic acid wool fibers

[0078] (1) Cutting

[0079] The curled hyaluronic acid wool fiber containing the stem cell culture medium component prepared in Example 2 was cut into a uniform length of 3 to 10 cm using a cutter.

[0080]

[0081] (2) Web formation by carding and airlaid

[0082] Hyaluronic acid wool fibers with cut curls were dispersed by air pressure and then injected by air pressure to form a web.

[0083]

[0084] (3) Needle punching

[0085] A multi-layer sheet formed from a web was needle-punched to produce a nonwoven fabric.

[0086]

[0087] (4) Hot pressing

[0088] The formed nonwoven fabric was pressurized at a certain temperature (70℃ or lower) to produce the final nonwoven fabric.

[0089]

[0090] Comparative Example 5: Nonwoven fabric manufacturing

[0091] A nonwoven fabric was prepared using the filament-type hyaluronic acid fiber prepared in Comparative Example 1 in the same manner as in Example 3.

[0092] As a result, it was confirmed that when using hyaluronic acid fibers with a smooth surface, the entanglement force between the fibers is weak during the needle punching process after web formation, so the nonwoven fabric is not formed well.

[0093]

[0094] Experimental Example 1: Measurement of Bulk Density of Hyaluronic Acid Fibers

[0095] 1 g each of the curled hyaluronic acid wool fiber prepared in Example 2, the filament-type hyaluronic acid fiber (Comparative Example 1), and the high-temperature static-dried hyaluronic acid fiber (Comparative Example 2) were prepared to have a length of 1 mm and a diameter of 0.0 mm, and after placing them into a 20 mL graduated cylinder, they were tapped 10 to 20 times or more with a plastic stick. Afterward, the bulk density was measured using the following formula, and the results are shown in Table 1.

[0096] Bulk density = Mass / Volume (g / ml)

[0097] Hyaluronic acid powder (g / ml) Hyaluronic acid fiber (g / ml) Comparative Example 1 Comparative Example 2 Example 2 0.5 80.3 80.3 30.31

[0098] From Table 1, the filament-type hyaluronic acid fiber of Comparative Example 1, which had a smooth surface, had a bulk density of 0.38 g / ml, while the hyaluronic acid fiber of Comparative Example 2, which was dried at high temperature and had a rough surface, had a bulk density of 0.33 g / ml, and the curled hyaluronic acid wool fiber prepared in Example 2, which had a rough surface, had a bulk density of 0.31 g / ml, indicating that secondary processing into nonwoven fabric was easy.

[0099]

[0100] Experimental Example 2: Evaluation of the Stability of Stem Cell Culture Medium Components

[0101] The stability of the active ingredient was evaluated by measuring the duration of the photoaging effect of the curl-impregnated hyaluronic acid wool fiber containing the stem cell culture medium component prepared in Example 2 and Comparative Example 4.

[0102] 1) Culture of human dermal fibroblasts

[0103] Human-derived skin fibroblasts were cultured in a 37°C incubator with a continuous supply of carbon dioxide using fibroblast culture medium (DMEM, 10% FBS, 1% A / A).

[0104] 2) Induction of photoaging in human dermal fibroblasts

[0105] To induce photoaging in cultured human-derived skin fibroblasts, six UVB lamps with a wavelength of 312 nm were exposed to the cells at an intensity of 100 mJ / cm². At this time, the exposure intensity of the UVB light source was monitored in real time through a UV detector installed inside the UVB irradiation device.

[0106] 3) Harsh treatment

[0107] Stem cell culture medium having the specifications of Fig. 2, and hyaluronic acid wool fibers with curls containing stem cell culture medium components (Examples 2 and Comparative Example 4) were left exposed for 24 hours at room temperature in a general laboratory without packaging or sealing.

[0108] 4) Anti-aging safety evaluation

[0109] The anti-aging effect on human-derived skin fibroblasts, which had undergone photoaging via UVB rays, was evaluated by treating them with a harsh-treated stem cell culture medium and a curled hyaluronic acid wool fiber containing stem cell culture medium components (Examples 2 and Comparative Example 4). In this case, the stem cell culture medium and hyaluronic acid wool fiber before harsh treatment were used as a control group. The anti-aging effect was quantitatively evaluated by selecting WST-8 for the quantitative evaluation of the proliferation rate of fibroblasts over time, and the detailed method is as follows. Human-derived skin fibroblasts were seeded at a rate of 4,000 per well in a 96-well plate and cultured in a cell culture incubator for 24 hours; afterward, the culture medium was removed and the cells were washed twice with PBS. After adding a small amount of PBS to prevent the cells from drying out, UVB irradiation was performed at a rate of 100 J / ㎠. Subsequently, PBS was removed and the FBS concentration was reduced to 1 / 100. The sample was added at an appropriate concentration (0.1-1 mg / mL) to the culture medium and then introduced into the cells. After culturing in an incubator for 24 hours, the final quantitative evaluation was completed by measuring cell viability using the Quanti-Max WST-8 cell viability assay kit (BioMax, QM5000). (Int J Oral Sci 14, 21. 10.1038, 2022. ACS Nano 14, 6887-6896. 2020. Aging Cell 21, 2022).

[0110]

[0111] Classification OD at 520nm UV Damaged Cells (Control Group) 0.31 Stem Cell Culture Medium Before Harsh Treatment 0.61 After Harsh Treatment 0.35 Comparative Example 1 Hyaluronic Acid Fiber Containing Stem Cell Culture Medium (Filament Type) Before Harsh Treatment 0.65 After Harsh Treatment 0.60 Example 2 Curled Hyaluronic Acid Wool Fiber Containing Stem Cell Culture Medium Before Harsh Treatment 0.65 After Harsh Treatment 0.60 Comparative Example 4 Curled Hyaluronic Acid Wool Fiber Containing Stem Cell Culture Medium (High-Temperature Blow-Dried) Before Harsh Treatment 0.45 After Harsh Treatment 0.40 Comparative Example 2 Hyaluronic Acid Fiber Containing Stem Cell Culture Medium (High-Temperature Stand-Dried) Before Harsh Treatment 0.38 After Harsh Treatment 0.36

[0112] From Table 2, the stem cell culture medium had an anti-aging effect before harsh treatment, but the anti-aging effect decreased after harsh treatment. On the other hand, the hyaluronic acid wool fiber containing the curled stem cell culture medium of Example 2 maintained an anti-aging effect not only before but also after harsh treatment. In addition, it was found that the hyaluronic acid fiber containing the stem cell culture medium that was static-dried at high temperature (Comparative Example 2) and the hyaluronic acid wool fiber containing the curled stem cell culture medium that was blow-dried at high temperature (Comparative Example 4) did not have a high anti-aging effect because the stem cell culture medium was damaged during the drying process.

[0113] Foregoing, specific parts of the present invention have been described in detail. It will be apparent to those skilled in the art that such specific descriptions are merely preferred embodiments and do not limit the scope of the invention. Accordingly, the actual scope of the invention is defined by the appended claims and their equivalents.

[0114]

[0115] The curled hyaluronic acid wool fiber of the present invention can be widely utilized in the fields of food, cosmetics, medical devices, and pharmaceuticals.

Claims

1. (a) A step of preparing a hyaluronic acid paste with a moisture content of 50 to 95% by weight by adding a solvent to a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for skin improvement, and mixing and kneading the mixture; (b) a step of extruding a hyaluronic acid paste with controlled moisture content through a nozzle at 15–70°C; and (c) A method for manufacturing curled hyaluronic acid wool fibers comprising the step of blow-drying an extruded spinning fluid at 30 to 70°C to form curled wool fibers.

2. A method for manufacturing a curled hyaluronic acid wool fiber according to claim 1, wherein the active ingredient for skin improvement is one or more selected from the group consisting of (a) a moisturizing and barrier strengthening ingredient, (b) an antioxidant ingredient, (c) a skin regeneration ingredient, (d) a skin soothing and anti-inflammatory ingredient, and (e) a whitening ingredient.

3. A method for manufacturing a curl-impregnated hyaluronic acid wool fiber, characterized in that, in paragraph 2, the skin-improving active ingredient is selected from the group consisting of vitamins, stem cell culture medium, and exosomes.

4. A method for manufacturing a curl-impregnated hyaluronic acid wool fiber, characterized in that, in addition to 100 parts by weight of the hyaluronic acid having a weight-average molecular weight of 50 to 3,000 kDa, the skin-improving active ingredient is 0.1 to 20 parts by weight.

5. A curled hyaluronic acid wool fiber comprising a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for skin improvement.

6. A nonwoven fabric comprising curled hyaluronic acid wool fibers containing a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for skin improvement.

7. A nonwoven fabric made of curled hyaluronic acid wool fiber, characterized in that, in claim 6, the skin-improving active ingredient is one or more selected from the group consisting of (a) moisturizing and barrier-strengthening ingredients, (b) antioxidant ingredients, (c) skin regeneration ingredients, (d) skin soothing and anti-inflammatory ingredients, and (e) whitening ingredients.

8. A wound dressing comprising a hyaluronic acid wool fiber with curls, comprising a hyaluronic acid salt having a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for skin improvement.

9. A shaping filler comprising a hyaluronic acid wool fiber with a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for skin improvement.

10. Cosmetics comprising curled hyaluronic acid wool fibers containing hyaluronic acid with a weight-average molecular weight of 50 to 3,000 kDa and an active ingredient for skin improvement.

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