Colorimetric detection device, system, and control method

Abstract

A colorimetric detection device, comprising a detection module (10) and an automatic liquid dispensing module (20), wherein the detection module (10) and the automatic liquid dispensing module (20) are configured to be detachably connected and fitted. The detection module (10) comprises: a support bottom plate (11); and at least one card slot plate mechanism (12) and an image acquisition unit (13). The card slot plate mechanism (12) is arranged on the support bottom plate (11) and is provided with at least one card slot (C), and the card slot (C) is used for loading a detection card (30). The image acquisition unit (13) is arranged above the card slot plate mechanism (12) so as to acquire image information of the detection card (30) in the card slot (C). The automatic liquid dispensing module (20) is used for transferring and dispensing a sample to the detection card (30) in the card slot (C). By using a modular design, the automatic liquid dispensing module (20) can be assembled on the basis of a manual colorimetric detection device, so as to upgrade the manual colorimetric detection device as an automatic colorimetric detection device. For different application environments, the modular design enables simplified device manufacturing and satisfies the requirements of customers for upgrade.

Description

Colorimetric detection equipment, systems and control methods

[0001] Cross-references to related applications

[0002] This application claims priority and benefit to Chinese patent application filed on January 26, 2025, application number 202510125583.9, entitled "Colorimetric Detection Equipment, System and Control Method", the contents of which are incorporated herein by reference in their entirety. Technical Field

[0003] This application belongs to the field of biological detection technology, and in particular relates to a colorimetric detection device, system and control method. Background Technology

[0004] In the field of biodetection technology, detection based on immunological and physicochemical reaction analysis is a common principle, leading to a series of technical products such as immunochromatography and chromogenic slides based on paper materials. These products have extensive applications in areas such as agricultural product food safety, in vitro diagnostics, and environmental monitoring.

[0005] These products mainly have two sample addition methods: manual addition and automatic addition. Currently, the manual addition product and the automatic addition product are independent products, and it is not possible to upgrade from the manual addition product to the automatic addition product. Summary of the Invention

[0006] This application provides a colorimetric detection device, system, and control method to solve the problem that existing dynamic liquid dispensing products cannot be upgraded to automatic liquid dispensing products.

[0007] According to one aspect of this application, a colorimetric detection device is provided, including a detection module and an automatic liquid dispensing module, wherein the detection module and the automatic liquid dispensing module are detachably connected and coupled. The detection module includes a supporting base plate, at least one card slot mechanism, and an image acquisition unit. The card slot mechanism is disposed on the supporting base plate and has at least one card slot for loading detection cards. The image acquisition unit is disposed above the card slot mechanism for acquiring image information of the detection cards in the card slots; the automatic liquid dispensing module is used for transferring and dispensing samples to the detection cards in the card slots.

[0008] In an optional embodiment of this application, the card slot plate mechanism includes a card slot plate and at least one pressing push rod; the card slot plate has at least one card slot, and each pressing push rod is disposed on the card slot plate and corresponds to one card slot. The pressing push rod is configured to eject the detection card along the extension direction of the card slot when a pushing force is applied toward the pressing push rod via the detection card in the card slot.

[0009] In an optional embodiment of this application, the card slot extends along the X direction, and the top of the two side walls of the card slot in the Y direction are formed with steps extending towards each other. The steps include a first stage and a second stage; the second stage protrudes from the first stage, and the lower side wall of the second stage is higher than the lower side wall of the first stage.

[0010] In an optional embodiment of this application, the card slot plate mechanism further includes at least one spring piece; each spring piece is correspondingly disposed on the bottom side of a card slot and is used to cooperate with the spring piece to clamp the detection card in the Z direction.

[0011] In the optional scheme of this application, there are multiple card slot plate mechanisms, which are arranged side by side in the Y direction, and each card slot plate mechanism is configured to load different types of test cards; or there is one card slot plate mechanism with multiple card slots arranged side by side in the Y direction, which are divided into multiple partitions arranged side by side in the Y direction, and each partition is configured to load different types of test cards.

[0012] In an optional embodiment of this application, the automatic liquid dispensing module includes a splicing base plate, a multi-axis module, a dispensing needle, and a liquid circuit assembly. The splicing base plate is detachably connected to a supporting base plate; the multi-axis module is mounted on the splicing base plate; the dispensing needle is connected to the multi-axis module and can switch between a liquid extraction position, a liquid injection position, and a cleaning position under the drive of the multi-axis module; the liquid circuit assembly is configured to extract liquid through the dispensing needle at the liquid extraction position, inject liquid through the dispensing needle at the liquid injection position, and clean the dispensing needle at the cleaning position.

[0013] In an optional embodiment of this application, the liquid injection needle includes a mounting block, an adjusting block, an inner needle, an outer needle, and an elastic element; the mounting block is connected to the multi-axis module, and the outer needle and the adjusting block are respectively connected to the two ends of the mounting block in the Z direction; the inner needle passes through the adjusting block, the mounting block, and the outer needle in the Z direction, and is able to move relative to the adjusting block, the mounting block, and the outer needle in the Z direction, with the end of the inner needle away from the adjusting block protruding from the end of the outer needle away from the adjusting block; the elastic element is located between the adjusting block and the mounting block, and the adjusting block is configured to be able to adjust the mounting position in the Z direction to control the compression amount of the elastic element, and the elastic element is configured to be compressed when the inner needle moves upward.

[0014] In the optional embodiment of this application, the liquid circuit assembly includes a sample station, a cleaning station, a pump station, and a switching valve; both the sample station and the pump station are mounted on the splicing base plate, and the cleaning station is mounted on the splicing base plate or the support base plate; the pump station is connected to the cleaning station, and the liquid injection needle is connected to the pump station through the switching valve, which is used to switch the pump in the pump station to which the liquid injection needle is connected.

[0015] In an optional embodiment of this application, the pump station includes a liquid addition pump, a waste liquid pump, a first cleaning pump, and a second cleaning pump; the liquid addition pump and the second cleaning pump are connected to the liquid addition needle via a switching valve, and both the first cleaning pump and the second cleaning pump are connected to the cleaning station; at the liquid extraction position, the liquid addition needle extends into the sample station and liquid is extracted by the liquid addition pump; at the cleaning position, the liquid addition needle extends into the cleaning station and cleaning reagent is delivered by the first cleaning pump and the second cleaning pump to clean the liquid addition needle; at the liquid injection position, the liquid addition needle extends into the detection card in a slot and liquid is injected by the liquid addition pump.

[0016] In an optional embodiment of this application, the sample station includes a sample holder, at least one heating plate, a cup holder, and multiple sample tubes; the sample holder is connected to the splicing base plate and is provided with multiple cup holder slots, each cup holder slot receiving at least a portion of the cup holder, and at least one of the multiple cup holder slots receiving a heating plate, the heating plate being located between the bottom wall of the cup holder slot and the cup holder; each cup holder is provided with an insertion hole for inserting a sample tube.

[0017] In an optional embodiment of this application, the automatic liquid dispensing module further includes at least one X-axis linear module; the X-axis linear module is disposed on the support base plate and connected to the card slot plate mechanism, so as to drive the card slot plate mechanism to drive the detection card to switch between the first position and the second position along the X direction.

[0018] In an optional embodiment of this application, the multi-axis module includes a Y-axis linear module and a Z-axis linear module; the Z-axis linear module is disposed on the splicing base plate, the Y-axis linear module is connected to the Z-axis linear module and located above the splicing base plate, and the liquid injection needle is connected to the Y-axis linear module; the Z-axis linear module can drive the Y-axis linear module to move the liquid injection needle up and down along the Z direction, and the Y-axis linear module can drive the liquid injection needle to move along the Y direction.

[0019] According to another aspect of this application, a colorimetric detection system is provided, including a host computer, a control module, and the aforementioned colorimetric detection device; the control module is disposed on the colorimetric detection device and connected to an image acquisition unit and an automatic liquid addition module, and the host computer is connected to the control module and is capable of communication.

[0020] According to another aspect of this application, a colorimetric detection control method is provided, applied to the aforementioned colorimetric detection system, and includes: acquiring detection card information of a detection card through an image acquisition unit; controlling an automatic liquid addition module to operate the detection card according to the detection card information, and obtaining a detection card after liquid addition is completed; acquiring image information of the detection card after liquid addition is completed through the image acquisition unit, and obtaining a detection result.

[0021] In summary, the colorimetric detection equipment, system, and control method provided in this application have at least the following beneficial effects:

[0022] This colorimetric detection device includes a detection module and an automatic liquid addition module, which are connected and detached via a snap-fit ​​mechanism. Without the automatic liquid addition module, sample detection can be achieved through manual liquid addition, making it a manual colorimetric detection device. With the automatic liquid addition module, sample detection can be achieved automatically, making it an automatic colorimetric detection device.

[0023] Thanks to its modular design and the detachable and compatible automatic liquid addition module and detection module, an automatic liquid addition module can be assembled on the basis of a manual colorimetric detection device to upgrade it into an automatic colorimetric detection device. In this way, the modular design simplifies the production of the equipment and meets the needs of customers for upgrades, catering to different application scenarios. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the specific embodiments of this application or the prior art, the accompanying drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application; those skilled in the art can obtain other drawings based on these drawings without any creative effort.

[0025] Figure 1 is a schematic diagram of a colorimetric detection device according to one embodiment of this application;

[0026] Figure 2 is a schematic diagram of a detection module provided according to one embodiment of this application;

[0027] Figure 3a is a schematic diagram of the slot plate mechanism in Figure 2;

[0028] Figure 3b is a schematic diagram of the slot plate mechanism in Figure 3a from another perspective;

[0029] Figure 3c is a schematic diagram of the slot plate mechanism in Figure 3a from another perspective;

[0030] Figure 4 is a schematic diagram of the colorimetric detection device in Figure 1 from another perspective;

[0031] Figure 5a is a schematic diagram of the assembly of the supporting base plate and the splicing base plate in Figure 4;

[0032] Figure 5b is an exploded view of the supporting base plate and the spliced ​​base plate in Figure 5a;

[0033] Figure 6 is a schematic diagram of the multi-axis module in Figure 4;

[0034] Figure 7 is a schematic diagram of the assembly of the X-axis linear module and the slot plate mechanism in Figure 4;

[0035] Figure 8a is a schematic diagram of the injection needle in Figure 4;

[0036] Figure 8b is an exploded view of the liquid injection needle in Figure 8a;

[0037] Figure 9 is a partial schematic diagram of a fluid circuit assembly provided according to one embodiment of this application;

[0038] Figure 10 is an exploded view of the sample station in Figure 4;

[0039] Figure 11 is a structural block diagram of a colorimetric detection system according to one embodiment of this application;

[0040] Figure 12 is a flowchart of the steps of a colorimetric detection control method according to one embodiment of this application.

[0041] The list of components represented by each number in the above attached diagram is as follows:

[0042] 100. Colorimetric detection equipment;

[0043] 10. Detection module;

[0044] 11. Support base plate; E. Buckle slot;

[0045] 12. Slot plate mechanism; 121a. First slot plate mechanism; 121b. Second slot plate mechanism; 121. Slot plate; C. Slot; 1211. First stage; 1212. Second stage; 1213. Linear module mounting interface; 122. Pressing rod; 123. Spring; 124. Switch;

[0046] 13. Image acquisition unit;

[0047] 14. Indicator light; 15. Outer casing sidewall; 16. Sliding column; 17. Bracket;

[0048] 20. Automatic liquid dispensing module;

[0049] 21. Splicing base plate; 211. Buckle;

[0050] 22. Multi-axis module; 221. Y-axis linear module; 2211. Y-axis crossbeam; 2212. Y-axis motor; 2213. Y-axis synchronous belt mechanism; 222. Z-axis linear module; 2221. Z-axis vertical beam; 2222. Z-axis motor; 2223. Z-axis synchronous belt mechanism;

[0051] 23. Fluid filling needle; 231. Mounting block; 232. Adjusting block; H4. Elongated hole; 233. Inner needle; 2331. Ring protrusion; 234. Outer needle; H5. Outer needle cleaning hole; 235. Elastic element; 236. Lubricating bushing;

[0052] 24. Hydraulic system assembly;

[0053] 241, Sample station; 2411, Sample holder; D, Cup holder slot; 2412, Heating plate; 2413, Cup holder; H1, Insertion hole; 2414, Sample tube;

[0054] 242. Cleaning station;

[0055] 243. Pumping station; 2431. Liquid filling pump; 2432. Waste liquid pump; 2433. First cleaning pump; 2434. Second cleaning pump;

[0056] 244. Switching valve;

[0057] 25. X-axis linear module; 251. X-axis linear motor; 252. X-axis guide rail slider mechanism;

[0058] 30. Test card; 31. First test card; H2. Gold cup well; H3. Sample well; 32. Second test card;

[0059] 400. Control module; 500. Host computer Detailed Implementation

[0060] In the description of this application, features specified with "first" or "second" are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Features specified with "first" or "second" may explicitly or implicitly include at least one of the specified features. The use of the term "multiple" generally means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0061] In this application, unless otherwise explicitly specified and limited, terms such as "installation," "connection," "joining," and "fixing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can be a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0062] In the description of this specification, the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that the specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0063] The “X direction”, “Y direction” and “Z direction” mentioned in this application are determined based on the rectangular coordinate system constructed by the colorimetric detection device 100. The “X direction” and “Y direction” together define the direction of the horizontal plane, and the “Z direction” is the direction perpendicular to the horizontal plane.

[0064] Figure 1 is a schematic diagram of a colorimetric detection device 100 according to one embodiment of the present application. Figure 2 is a schematic diagram of a detection module 10 according to one embodiment of the present application.

[0065] Please refer to Figures 1 and 2. The colorimetric detection device 100 includes a detection module 10 and an automatic liquid addition module 20. The detection module 10 and the automatic liquid addition module 20 are configured to be connected and disassembled in a snap-fit ​​manner.

[0066] In this embodiment, with only the detection module 10, the liquid addition operation can be performed by an operator, i.e., manual liquid addition. By adding an automatic liquid addition module 20 to the detection module 10, the automatic liquid addition function can be realized.

[0067] Furthermore, the detection module 10 includes a supporting base plate 11, at least one card slot mechanism 12, and an image acquisition unit 13. The card slot mechanism 12 is disposed on the supporting base plate 11 and has at least one card slot C for loading the detection card 30. The image acquisition unit 13 is disposed above the card slot mechanism 12 to acquire image information of the detection card 30 in the card slot C.

[0068] It should be noted that the test card 30 here can generate a visual result based on color or pattern changes according to the dropped sample, in order to determine whether the sample contains the target substance and its concentration level. In specific applications, the test card 30 may include, for example, immunochromatographic test strips or chromogenic slides.

[0069] The support base plate 11 provides support for other components in the detection module 10, and the card slot plate mechanism 12 has a card slot C, in which the detection card 30 can be installed.

[0070] It should be noted that, in the above, manual liquid addition refers to the operator dripping the sample onto the test card 30 located in the card slot C. Furthermore, the automatic liquid addition module 20 is used to transfer and add samples to the test card 30 in the card slot C. Automatic liquid addition refers to transferring the sample via the automatic liquid addition module 20 and dripping the sample onto the test card 30 located in the card slot C.

[0071] Next, the image information on the detection card 30 can be acquired through the image acquisition unit 13 for result analysis. The image information here can be the color, pattern, etc. on the detection card 30.

[0072] As can be seen from the above, for this colorimetric detection device 100, without the automatic liquid addition module 20, sample detection can be achieved through manual liquid addition, which is a manual colorimetric detection device. With the automatic liquid addition module 20, sample detection can be achieved through automatic liquid addition, which is an automatic colorimetric detection device.

[0073] Due to its modular design and the fact that the automatic liquid addition module 20 and the detection module 10 can be detached and connected, the automatic liquid addition module 20 can be assembled on the basis of the manual colorimetric detection equipment to upgrade it into an automatic colorimetric detection equipment. In this way, the modular design achieves the simplicity of equipment production and meets the needs of customer upgrades for different application scenarios.

[0074] In one optional embodiment, there are multiple card slot plate mechanisms 12, which are arranged side by side in the Y direction, and each card slot plate mechanism 12 is configured to load different types of detection cards 30.

[0075] In this embodiment, there are multiple card slot plate mechanisms 12. Each card slot C on the same card slot plate mechanism 12 can be used to load the same type of detection card 30, while different card slot plate mechanisms 12 are used to load different detection cards.

[0076] In the embodiment shown in Figure 2, there are two card slot plate mechanisms 12, which are respectively the first card slot plate mechanism 12a and the second card slot plate mechanism 12b. The two card slot plate mechanisms 12 are arranged side by side in the Y direction. The first card slot plate mechanism 12a and the second card slot plate mechanism 12b each have 4 card slots C. Preferably, the first card slot plate mechanism 12a and the second card slot plate mechanism 12b cannot be loaded with the same type of detection card 30, so that two kinds of samples can be detected at the same time.

[0077] Of course, the first card slot mechanism 12a and the second card slot mechanism 12b can also use the same type of detection card 30 to increase the throughput of a single sample. Understandably, for a card slot mechanism 12, the detection throughput can be increased by increasing the number of card slots C, and the number of card slot mechanisms 12 can be increased to increase the number of compatible samples. In this way, multiple samples can be detected while ensuring a high detection throughput.

[0078] In another optional embodiment, the number of card slot plate mechanisms 12 is one and it is provided with multiple card slots C arranged side by side along the Y direction. The multiple card slots C are divided into multiple partitions arranged side by side in the Y direction, and each partition is configured to load different types of detection cards 30.

[0079] In this embodiment, there is only one card slot plate mechanism 12 and it has multiple card slots C. These card slots C are divided into multiple regions. Each card slot C in the same region is loaded with the same type of detection card 30, and different regions are loaded with different types of detection cards 30.

[0080] For example, the card slot plate mechanism 12 has eight card slots C arranged side by side in the Y direction and is divided into two areas arranged side by side in the Y direction, so that two types of detection cards 30 can be loaded, and each area has four card slots C, and four of a single type of detection card 30 can be loaded at one time.

[0081] Compared with the two embodiments, the arrangement of multiple card slot plate mechanisms 12 is preferred. This allows for the loading of another type of detection card 30 while one type of detection card 30 is being tested, providing greater flexibility and enabling on-demand testing.

[0082] Figure 3a is a schematic diagram of the slot plate mechanism 12 in Figure 2. Figure 3b is a schematic diagram of the slot plate mechanism 12 in Figure 3a from another perspective. Figure 3c is a schematic diagram of the slot plate mechanism 12 in Figure 3a from yet another perspective.

[0083] Referring to Figures 3a to 3c, the slot plate mechanism 12 includes a slot plate 121 and at least one pressing push rod 122. The slot plate 121 has at least one slot C, and each pressing push rod 122 is disposed on the slot plate 121 and corresponds to one slot C. The pressing push rod 122 is configured to eject the detection card 30 along the extending direction of the slot C when a pushing force is applied toward the pressing push rod 122 via the detection card 30 in the slot C.

[0084] In this embodiment, a slot C is formed on the slot plate 121 and a pressing push rod 122 is installed thereon. The number of pressing push rods 122 is equal to the number of slots C and they are arranged in alignment.

[0085] In one embodiment, each pressing push rod 122 is disposed on the same side of each card slot C in the extension direction, and the detection card 30 can be inserted into the card slot C along the extension direction of the card slot C and abut against the corresponding pressing push rod 122.

[0086] If the test card 30 is pressed further to apply a pushing force to the push rod 122, the push rod 122 will pop out the test card 30, making it easier for the operator to replace the test card 30.

[0087] It should be noted that the push rod 122 is widely used in the mechanical field, and will not be described in detail here. Additionally, in this embodiment, the extension direction of the card slot C is the X-direction, which is also the insertion direction of the detection card 30.

[0088] In a further optional embodiment, the slot C extends along the X direction, and the top of the Y-direction sidewalls of the slot C are formed with steps extending toward each other, the steps including a first stage 1211 and a second stage 1212. The second stage 1212 protrudes beyond the first stage 1211, and the lower sidewall of the second stage 1212 is higher than the lower sidewall of the first stage 1211.

[0089] In this embodiment, the second stage 1212 protrudes further inward than the first stage 1211, and the lower sidewall of the second stage 1212 is higher than the lower sidewall of the first stage 1211.

[0090] In one embodiment, the detection card 30 has two types of detection cards, corresponding to the first detection card 31 and the second detection card 32 respectively. The width of the first detection card 31 is narrower than that of the second detection card 32, and the thickness of the first detection card 31 is thicker than that of the second detection card 32. Thus, the first detection card 31 can be limited by the spring piece 123 in conjunction with the lower side wall of the second stage 1212, and the second detection card 32 can be limited by the spring piece 123 in conjunction with the lower side wall of the first stage 1211.

[0091] Thus, the card slot plate mechanism 12 can be used with at least two types of detection cards 30. It should be noted that the relative relationship between inside and outside is determined based on the center of the card slot C. The center that is relatively closer to the center of the card slot C is considered inside, and the center that is relatively farther away from the center of the card slot C is considered outside.

[0092] In a further optional embodiment, the card slot plate mechanism 12 further includes at least one spring piece 123; each spring piece 123 is correspondingly disposed on the bottom side of a card slot C, and is used to cooperate with the spring piece 123 to clamp the detection card 30 in the Z direction.

[0093] In this embodiment, a spring piece 123 is installed at the bottom of each card slot C. The spring piece 123 can cooperate with the steps on both sides of the top of the card slot C to hold the detection card 30 and prevent the detection card 30 from sliding in the card slot C.

[0094] In particular, since the spring 123 can prevent the detection card 30 from sliding, it can reduce the risk of image acquisition deviation caused by the slippage of the detection card 30 during the sample transfer process.

[0095] In one alternative embodiment, the spring 123 is arched upwards, with the highest point of the spring 123 extending beyond the bottom wall of the slot C. As the detection card 30 is inserted, the spring 123 is compressed, thereby enabling the upper side wall of the detection card 30 to abut against the lower side wall of the step, thus achieving a snap-fit.

[0096] In some optional embodiments, the detection module 10 further includes at least one indicator light 14, each indicator light 14 being disposed above the card slot plate mechanism 12 and corresponding to a card slot C.

[0097] The card slot plate mechanism 12 also includes at least one switch 124, each switch 124 being triggered by a detection card 30 disposed in the card slot C and configured to be pushed into the card slot C to switch on the corresponding indicator light 14.

[0098] In this embodiment, the detection module 10 also includes an indicator light 14, which indicates to the operator whether the corresponding card slot C is loaded with a detection card 30 by turning the indicator light 14 on or off.

[0099] Specifically, each card slot C is equipped with a corresponding switch 124, and the detection card 30 can come into contact with the switch 124 during the insertion of the card slot C, thereby triggering the switch 124 to turn on and off.

[0100] For example, when a detection card 30 is loaded in card slot C, the indicator light 14 corresponding to card slot C is lit. When a detection card 30 is not loaded in card slot C, the indicator light 14 corresponding to card slot C is off.

[0101] In the illustrated embodiment, there are 8 card slots C and 8 indicator lights 14, which are aligned one by one.

[0102] In a further optional embodiment, the slot plate 121 is arranged at intervals above the support base plate 11, and the bottom side of the slot plate 121 is provided with a linear module mounting interface 1213.

[0103] In this embodiment, the linear module mounting interface 1213 on the bottom side of the slot plate 121 is used to mount the X-axis linear module 25 to quickly assemble the X-axis linear module 25, thereby facilitating the upgrade from manual to automatic colorimetric detection equipment.

[0104] In one optional embodiment, the image acquisition unit 13 includes a camera and a lighting lamp. The lighting lamp provides illumination to facilitate the camera in acquiring clear image information, ensuring the accuracy of the result analysis. The camera may be an industrial camera, including, for example, a CCD camera or a CMOS camera.

[0105] Figure 4 is a schematic diagram of the colorimetric detection device 100 in Figure 1 from another perspective. Referring to Figure 4, the automatic liquid dispensing module 20 includes a splicing base plate 21, a multi-axis module 22, a liquid dispensing needle 23, and a liquid circuit assembly 24.

[0106] The splicing base plate 21 is detachably connected to the supporting base plate 11. The multi-axis module 22 is disposed on the splicing base plate 21. The liquid injection needle 23 is connected to the multi-axis module 22 and can switch between the liquid extraction position, the liquid injection position, and the cleaning position under the drive of the multi-axis module 22.

[0107] The fluid assembly 24 is configured to draw liquid through the filling needle 23 at the drawing position, inject liquid through the filling needle 23 at the injection position, and clean the filling needle 23 at the cleaning position.

[0108] In this embodiment, the splicing base plate 21 can provide support for other components of the automatic liquid addition module 20, and the splicing base plate 21 is detachably connected to the support base plate 11.

[0109] Figure 5a is an assembly diagram of the supporting base plate 11 and the splicing base plate 21 in Figure 4. Figure 5b is an exploded view of the supporting base plate 11 and the splicing base plate 21 in Figure 5a.

[0110] In the embodiments shown in Figures 5a and 5b, the supporting base plate 11 and the splicing base plate 21 can be spliced ​​in the Y direction. Specifically, the supporting base plate 11 has a plurality of snap-fit ​​grooves E arranged at intervals along the X direction on the Y direction side facing the splicing base plate 21, and the splicing base plate 21 has a plurality of snap-fits 211 arranged at intervals along the X direction on the Y direction side facing the supporting base plate 11. The plurality of snap-fits 211 can be inserted into the plurality of snap-fit ​​grooves E one by one, and the bottom side of the snap-fits 211 and the snap-fit ​​grooves E form aligned holes to cooperate with bolts (such as bolts, screws) to achieve a detachable connection.

[0111] It should be noted that the detachable connection between the support base plate 11 and the splicing base plate 21 is not limited to the method shown in the figure, which is achieved by using a snap-fit ​​and slot with a screw. For example, bolt connection or pin connection can also be used directly.

[0112] The multi-axis module 22 provides the injection needle 23 with at least two degrees of freedom of movement in two directions, thereby enabling the injection needle 23 to move to the corresponding working position, which includes the liquid extraction position, the liquid injection position, and the cleaning position.

[0113] Figure 6 is a schematic diagram of the multi-axis module 22 in Figure 4. Referring to Figure 6, the multi-axis module 22 includes a Y-axis linear module 221 and a Z-axis linear module 222.

[0114] The Z-axis linear module 222 is mounted on the splicing base plate 21, the Y-axis linear module 221 is connected to the Z-axis linear module 222 and is located above the splicing base plate 21, and the liquid injection needle 23 is connected to the Y-axis linear module 221.

[0115] The Z-axis linear module 222 can drive the Y-axis linear module 221 to move the liquid injection needle 23 up and down along the Z direction, and the Y-axis linear module 221 can drive the liquid injection needle 23 to move along the Y direction.

[0116] In this embodiment, the multi-axis module 22 is a dual-axis linear module, specifically composed of a Y-axis linear module 221 and a Z-axis linear module 222. The liquid injection needle 23 is mounted on the Y-axis linear module 221 and has Y-axis and Z-axis movement degrees of freedom under the combined drive of the Y-axis linear module 221 and the Z-axis linear module 222.

[0117] In the embodiment shown in Figure 6, both the Y-axis linear module 221 and the Z-axis linear module 222 are synchronous belt linear modules. Specifically, the Z-axis linear module 222 includes a Z-axis upright beam 2221, a Z-axis motor 2222, and a Z-axis synchronous belt mechanism 2223. The Z-axis upright beam 2221 is mounted on the splicing base plate 21. The Z-axis motor 2222 is mounted at the lower end of the Z-axis upright beam 2221 and connected to the Z-axis synchronous belt mechanism 2223. It can drive the Z-axis synchronous belt mechanism 2223 to move the Y-axis linear module 221 connected to the Z-axis synchronous belt mechanism 2223 up and down in the Z direction.

[0118] Furthermore, the Y-axis linear module 221 includes a Y-axis crossbeam 2211, a Y-axis motor 2212, and a Y-axis synchronous belt mechanism 2213. The Y-axis crossbeam 2211 is connected to the Z-axis synchronous belt mechanism 2223 and slidably connected to the Z-axis vertical beam 2221, so that it can move up and down relative to the Z-axis vertical beam 2221 in the Z-direction under the drive of the Z-axis synchronous belt mechanism 2223.

[0119] The liquid injection needle 23 is connected to the Y-axis synchronous belt mechanism 2213 and slidably connected to the Y-axis crossbeam 2211. The Y-axis motor 2212 is located at the Y-axis end of the Y-axis crossbeam 2211 near the Z-axis linear module 222 and is connected to the Y-axis synchronous belt mechanism 2213 to drive the Y-axis synchronous belt mechanism 2213 to move the liquid injection needle 23 along the Y-axis.

[0120] Thus, the injection needle 23, driven by the two linear modules, possesses both Y-axis and Z-axis degrees of freedom. It should be noted that the synchronous belt mechanism comprises a synchronous belt, a drive wheel, and a follower wheel. The drive wheel and follower wheel are spaced apart, and a synchronous belt connects them. The wheel connected to the motor serves as the drive wheel. The synchronous belt mechanism is a transmission mechanism well-known to those skilled in the art and will not be described in detail here. Furthermore, the multi-axis module 22 is not limited to the combination of synchronous belt linear modules shown in the figure; for example, it can also employ combinations of ball screw linear modules, linear motors, etc.

[0121] Referring to Figure 4, in one embodiment, the supporting base plate 11 is provided with a sliding column 16. The sliding column 16 and the Z-axis vertical beam 2221 in the Z-axis linear module 222 are arranged at intervals in the Y direction. The end of the Y-axis horizontal beam 2211 in the Y-axis linear module 221 away from the Z-axis linear module 222 is slidably connected to the sliding column 16.

[0122] Thus, the Z-axis vertical beam 2221, the Y-axis horizontal beam 2211, and the sliding column 16 form a gantry structure, ensuring the reliability of the multi-axis module 22. Alternatively, the sliding column 16 can be omitted, meaning the Y-axis horizontal beam 2211 would only have support at one end, adopting a cantilever structure. Correspondingly, the Y-axis horizontal beam 2211 would require a reinforced structural design, such as using high-strength materials (e.g., ultra-hard aluminum alloy) or changing the cross-sectional shape to improve strength.

[0123] Figure 7 is a schematic diagram of the assembly of the X-axis linear module 25 and the slot plate mechanism 12 in Figure 4. Referring to Figure 7, in a further optional embodiment, the automatic liquid dispensing module 20 also includes at least one X-axis linear module 25. The X-axis linear module 25 is disposed on the support base plate 11 and connected to the slot plate mechanism 12 to drive the slot plate mechanism 12 to switch the detection card 30 between a first position and a second position along the X direction.

[0124] In this embodiment, the X-axis linear module 25 can provide X-axis movement freedom, so that the slot plate mechanism 12 can move in the X-axis and switch between the first position and the second position.

[0125] In practical applications, the X-axis linear module 25, in conjunction with the multi-axis module 22, can confirm the injection position. Therefore, there are multiple injection positions.

[0126] In one embodiment, the first detection card 31 has a gold cup hole H2 and a sample hole H3. When the card slot plate mechanism 12 is in the first position, the liquid injection needle 23 can move above the gold cup hole H2 under the drive of the multi-axis module 22 to inject liquid into the gold cup hole H2. When the card slot plate mechanism 12 is in the second position, the liquid injection needle 23 can move above the sample hole H3 under the drive of the multi-axis module 22 to inject liquid into the sample hole H3.

[0127] In the embodiment shown in Figure 7, the X-axis linear module 25 is connected to the linear module mounting interface 1213 and includes an X-axis linear motor 251 and an X-axis guide rail slider mechanism 252 arranged at intervals in the Y direction. That is, the X-axis linear module 25 is a guide rail type linear module, but it is not limited to this; for example, it can also be a lead screw type linear module.

[0128] Referring to Figures 2 and 7, for the automatic colorimetric detection equipment, the slot plate mechanism 12 is supported by the X-axis linear module 25 and is arranged at intervals with the support base plate 11 in the Z direction. For the manual colorimetric detection equipment, the slot plate mechanism 12 is supported by the bracket 17 and is arranged at intervals with the support base plate 11 in the Z direction.

[0129] Understandably, to upgrade a manual to an automatic colorimetric detection device, the bracket 17 can be removed, and then the X-axis linear module 25 can be matched with the linear module mounting interface 1213 to provide the slot plate mechanism 12 with X-axis freedom of movement.

[0130] In one alternative embodiment, the X-axis linear module 25 may be integrated into the multi-axis module 22, so that the multi-axis module 22 can provide three degrees of freedom. Accordingly, the structure of the multi-axis module 22 is more complex, resulting in a relatively large overall device.

[0131] In this embodiment, the X-axis linear module 25 is separated and arranged between the slot plate mechanism 12 and the support base plate 11, which can ensure that the whole device is more compact, that is, it is conducive to the miniaturization design of the device.

[0132] Figure 8a is a schematic diagram of the liquid filling needle 23 in Figure 4. Figure 8b is an exploded view of the liquid filling needle 23 in Figure 8a. Referring to Figures 8a and 8b, the liquid filling needle 23 includes a mounting block 231, an adjusting block 232, an inner needle 233, an outer needle 234, and an elastic element 235. The mounting block 231 is connected to the multi-axis module 22, and the outer needle 234 and the adjusting block 232 are respectively connected to the two Z-axis ends of the mounting block 231.

[0133] The inner needle 233 passes through the adjusting block 232, the mounting block 231 and the outer needle 234 in the Z direction, and can move relative to the adjusting block 232, the mounting block 231 and the outer needle 234 in the Z direction. The end of the inner needle 233 away from the adjusting block 232 protrudes from the end of the outer needle 234 away from the adjusting block 232.

[0134] The elastic element 235 is located between the adjusting block 232 and the mounting block 231. The adjusting block 232 is configured to adjust the mounting position in the Z direction to control the compression amount of the elastic element 235. The elastic element 235 is configured to be compressed when the inner needle 233 moves upward.

[0135] In this embodiment, the liquid injection needle 23 adopts a double-layer structure with inner and outer sleeves. Specifically, it has an inner needle 233 and an outer needle 234, with the outer needle 234 sleeved over the inner needle 233.

[0136] The outer needle 234 and the adjusting block 232 are respectively installed at the lower and upper ends of the mounting block 231, i.e., at both ends in the Z direction. The inner needle 233 passes through the adjusting block 232, the mounting block 231, and the outer needle 234. Specifically, the outer needle 234 has a hollow structure, and both the adjusting block 232 and the mounting block 231 are provided with through holes to cooperate with the outer needle 234 to form a channel that allows the inner needle 233 to pass through.

[0137] Furthermore, the inner needle 233 can move up and down relative to the adjusting block 232, the mounting block 231 and the outer needle 234 in the 2nd position, that is, the inner needle 233 is in sliding connection with the adjusting block 232, the mounting block 231 and the outer needle 234.

[0138] Furthermore, the end furthest from the adjusting block 232 corresponds to the lower end, meaning the lower end of the inner needle 233 protrudes beyond the lower end of the outer needle 234. Thus, during the process of moving the injection needle 23 to the injection position, the inner needle 233 contacts the detection card 30 before the outer needle 234. Because there is an elastic element 235 between the adjusting block 232 and the mounting block 231, and this elastic element 235 is compressed when the inner needle 233 moves upward, the inner needle 233 contacts the detection card 30 before being compressed, until the outer needle 234 then contacts the detection card 30.

[0139] In one optional embodiment, the end of the outer needle 234 furthest from the adjusting block 232 is formed with a pointed tip, that is, the lower end of the outer needle 234 is a pointed tip. It should be noted that, for the first detection card 31, the gold cup hole H2 contains colloidal gold-labeled bioactive substances. In order to avoid contamination of these bioactive substances, the top of the gold cup hole H2 is generally provided with a sealing film layer.

[0140] Since the lower end of the inner needle 233 is not a pointed tip, although the inner needle 233 contacts the sealing film layer on the gold cup hole H2 first during the downward movement of the liquid injection needle 23, the lower end of the inner needle 233 cannot pierce through it, which causes the inner needle 233 to move upward, and the elastic element 235 is compressed until the outer needle 234 contacts the sealing film layer on the gold cup hole H2 and pierces through the sealing film layer.

[0141] Since the inner needle 233 has no sealing membrane layer to obstruct it, it is ejected by the elastic force of the elastic element 235 and then extends into the gold cup orifice H2, thus allowing liquid injection. It should be noted that the inner needle 233 is also a hollow structure, forming a channel that allows sample flow.

[0142] Thus, by adopting a double-layer needle structure with an elastic element, the inner needle 233 can extend and retract and contact the detection card 30 before the outer needle 234, and the lower end of the outer needle 234 forms a puncture tip to avoid contaminating the bioactive substances in the detection card 30 during the liquid injection process.

[0143] In a further optional embodiment, the outer peripheral wall of the inner needle 233 is provided with an annular protrusion 2331, and the elastic member 235 is sleeved on the inner needle 233 and sandwiched between the annular protrusion 2331 and the adjusting block 232.

[0144] In this embodiment, the elastic element 235 is sleeved on the inner needle 233, with its lower end abutting against the annular protrusion 2331 and its upper end abutting against the adjusting block 232. Understandably, as the inner needle 233 moves upward, the annular protrusion 2331 moves upward, thereby compressing the elastic element 235.

[0145] It should be noted that the number of elastic elements 235 is not limited to one and the installation method of inner pin 233 is not adopted. For example, there can be multiple elastic elements 235, which are arranged circumferentially around the inner pin 233, and the two ends of each elastic element 235 abut against the annular protrusion 2331 and the adjusting block 232, respectively. In the illustrated embodiment, the elastic element 235 is a spring, but it is not limited to this. For example, elastic rubber pads can also be used.

[0146] In one optional embodiment, the outer peripheral wall of the outer needle 234 is provided with a plurality of outer needle cleaning holes H5 to allow cleaning agent to pass through. Understandably, the lower end of the inner needle 233 is the part that contacts the sample and active reagent, and arranging these outer needle cleaning holes H5 close to the bottom facilitates cleaning of the outer peripheral wall of the inner needle 233.

[0147] In the embodiment shown in Figure 8b, the adjusting block 232 is provided with parallel, spaced elongated holes H4 extending along the Z-direction. These holes H4, in conjunction with the holes on the mounting block 231, allow for fine-tuning of the mounting positions of the adjusting block 232 and the mounting block 231 in the Z-direction, thereby adjusting the compression of the elastic element 235. It should be noted that, preferably, the mounting position of the adjusting block 232 ensures that the elastic element 235 is in a slightly compressed state, that is, ensures that the elastic element 235 has a certain amount of compression and maintains a certain elastic force.

[0148] In one optional embodiment, both the mounting block 231 and the adjusting block 232 are provided with a lubricating bushing 236, which is sleeved over the inner needle 233.

[0149] In this embodiment, a lubricating bushing 236 is provided at the hole through which the inner needle 233 passes by the mounting block 231 and the adjusting block 232. The lubricating bushing 236 reduces the friction generated during the movement of the inner needle 233, thereby ensuring the service life of the related components of the liquid injection needle 23.

[0150] Preferably, the lubricating bushing 236 is a self-lubricating bushing, for example, a bushing made of a material with self-lubricating function such as polytetrafluoroethylene.

[0151] Figure 9 is a partial schematic diagram of a fluid circuit assembly according to one embodiment of this application. Referring to Figure 9 and Figure 4, in some optional embodiments, the fluid circuit assembly 24 includes a sample station 241, a cleaning station 242, a pump station 243, and a switching valve 244. The sample station 241 and the pump station 243 are both disposed on the splicing base plate 21, and the cleaning station 242 is disposed on the splicing base plate 21 or the support base plate 11.

[0152] Pump station 243 is connected to cleaning station 242. Liquid injection needle 23 is connected to pump station 243 through switching valve 244. Switching valve 244 is used to switch the pump in pump station 243 to which liquid injection needle 23 is connected.

[0153] In this embodiment, the sample station 241 is used to hold the sample to be injected. The injection needle 23 moves under the drive of the multi-axis module 22 to the position of the sample station 241 or the slot of the detection card 30 to be injected, which corresponds to the injection position.

[0154] The cleaning station 242 is used to hold cleaning agent for cleaning the liquid injection needle. The liquid injection needle 23 moves to the position of the cleaning station 242 under the drive of the multi-axis module 22, which is the cleaning position.

[0155] Driven by the multi-axis module 22, the liquid injection needle 23 moves to the slot position corresponding to the liquid injection position of the detection card 30 to be injected. It should be understood that there are multiple liquid extraction positions and multiple liquid injection positions. Except for the liquid extraction position corresponding to the position of the sample station 241, the liquid extraction positions and liquid injection positions of other parts coincide.

[0156] As can be seen from the above, the sample station 241, the cleaning station 242 and the card slot plate mechanism 12 are within the moving coverage range of the liquid injection needle 23. In the illustrated embodiment, the sample station 241, the cleaning station 242 and the card slot plate mechanism 12 are arranged at intervals in the Y direction, and the cleaning station 242 is installed on the support base plate 11.

[0157] Understandably, the cleaning station 242 can also be installed on the splicing base plate 21 by appropriately adjusting the shape of the splicing base plate 21.

[0158] Furthermore, pump station 243 is connected to cleaning station 242 and liquid injection needle 23, and a switching valve 244 is also provided between pump station 243 and liquid injection needle 23. It should be noted that pump station 243 includes multiple pumps, each with a different function. The switching valve 244 can switch the connection between different pumps in pump station 243 and liquid injection needle 23, thereby realizing different functions such as liquid pumping, liquid injection, and cleaning.

[0159] In a further optional embodiment, pump station 243 includes a liquid filling pump 2431, a waste liquid pump 2432, a first cleaning pump 2433, and a second cleaning pump 2434. The liquid filling pump 2431 and the second cleaning pump 2434 are connected to the liquid filling needle 23 via a switching valve 244, and both the first cleaning pump 2433 and the second cleaning pump 2434 are connected to the cleaning station 242.

[0160] At the aspiration position, the dosing needle 23 extends into the sample station 241 and aspirates liquid via the dosing pump 2431. At the cleaning position, the dosing needle 23 extends into the cleaning station 242 and is cleaned by cleaning reagent delivered by the first cleaning pump 2433 and the second cleaning pump 2434. At the injection position, the dosing needle 23 extends into the detection card 30 in a slot C and is injected with liquid via the dosing pump 2431.

[0161] In this embodiment, the pump station 243 has at least four pumps, namely, a liquid addition pump 2431, a waste liquid pump 2432, a first cleaning pump 2433, and a second cleaning pump 2434.

[0162] The liquid injection pump 2431 performs liquid injection and liquid extraction operations. The first cleaning pump 2433 provides cleaning agent to the cleaning station 242 to clean the outer peripheral walls of the outer needle 234 and the inner needle 233. The second cleaning pump 2434 provides cleaning agent to the inner needle 233 to clean the inner peripheral wall of the inner needle 233.

[0163] Understandably, after the injection needle 23 injects the sample into the corresponding test card 30, it needs to be cleaned to avoid cross-contamination, which could affect the test results.

[0164] It should be understood that the injection and aspiration actions and the cleaning of the inner peripheral wall of the inner needle 233 cannot be performed simultaneously. Therefore, the switching valve 244 is used to switch the connection between the liquid injection pump 2431 and the liquid injection needle 23, as well as between the second cleaning pump 2434 and the liquid injection needle 23.

[0165] It should be noted that both the first cleaning pump 2433 and the second cleaning pump 2434 are connected to pipelines providing cleaning reagents, and the waste liquid pump 2432 is connected to a waste liquid discharge pipeline to extract the waste liquid generated during cleaning in the cleaning station 242. In practical applications, the waste liquid pump 2432, the first cleaning pump 2433, and the second cleaning pump 2434 are all peristaltic pumps, and the liquid injection pump 2431 needs to be able to precisely control the injection volume, therefore a plunger pump is used.

[0166] Figure 10 is an exploded view of sample station 241 in Figure 4. Referring to Figure 10, sample station 241 includes sample holder 2411, at least one heating plate 2412, cup holder 2413, and multiple sample tubes 2414.

[0167] The sample holder 2411 is connected to the splicing base plate 21 and has multiple cup holder slots D. Each cup holder slot D accommodates at least a portion of a cup holder 2413. At least one of the multiple cup holder slots D accommodates a heating plate 2412, which is located between the bottom wall of the cup holder slot D and the cup holder 2413. The cup holder 2413 has an insertion hole H1 for inserting a sample tube 2414.

[0168] In this embodiment, there are multiple sample tubes 2414, each sample tube 2414 can hold one type of sample, and multiple sample tubes 2414 can hold multiple types of samples.

[0169] The sample holder 2411 is mounted on the splicing base plate 21 and is provided with multiple cup holder slots D, which are used to install cup holders 2413.

[0170] In the embodiment shown in Figure 10, there are multiple cup holders 2413. Each cup holder slot D has a corresponding cup holder 2413 installed and a portion of the cup holder 2413 is accommodated. Each cup holder 2413 is provided with a plug hole H1, into which the sample tube 2414 can be inserted.

[0171] As can be seen, the number of cup holders D, the number of sample tubes 2414, and the number of cup holders 2413 are the same, with one cup holder 2413 supporting one sample tube 2414. Furthermore, the sample station 241 may also include a heating plate 2412. The number of heating plates 2412 generally cannot exceed the number of sample tubes 2414, and they are installed on the bottom wall of the cup holder D and sandwiched between the cup holder D and the cup holders 2413.

[0172] It should be noted that the depth of the cup holder D, which requires the heating plate 2412 to be installed, is increased to facilitate the housing of the heating plate 2412 and to ensure the height alignment of each sample tube 2414.

[0173] In one optional embodiment, the number of cup holders 2413 is one, and the cup holder 2413 is provided with at least two insertion holes H1, which can support multiple sample tubes 2414 of the same size or different sizes.

[0174] In one optional embodiment, the number of cup holders 2413 is one, and the cup holder 2413 is provided with at least two insertion holes H1, and the materials corresponding to the different insertion holes H1 are different.

[0175] For example, the cup holder 2413 has two insertion holes H1, and the cup holder 2413 is divided into a heating zone made of a metal thermally conductive material and a non-heating zone made of a heat-insulating material. The heating zone and the non-heating zone are each provided with an insertion hole H1. Of course, the heating zone works with the heating plate 2412 to heat the sample tube 2414 inserted therein, while the sample tube 2414 in the non-heating zone cannot be heated.

[0176] In one alternative embodiment, the heating plate 2412 may employ an electric heating element, including, for example, an electric heating plate, a PTC heating element, a film electric heating element, etc.

[0177] In one optional embodiment, the portion of the cup holder 2413 that protrudes from the cup holder groove D corresponds to the handle portion, which is designed to be easy for fingers to grip, so that staff can easily pull out and replace the sample tube 2414 containing different samples.

[0178] In the illustrated embodiment, the sample station 241 contains two sample tubes 2414, one of which is equipped with a heating plate 2412 to heat the sample inside. However, the number of sample tubes 2414 and heating plates 2412 in the sample station 241 is not limited to the illustrated embodiment and can be adjusted appropriately. Preferably, the number of sample tubes 2414 in the sample station 241 is equal to the number of slot plate mechanisms 12.

[0179] Figure 11 is a structural block diagram of a colorimetric detection system according to one embodiment of this application. Referring to Figure 11, the colorimetric detection system includes a host computer 500, a control module 400, and the aforementioned colorimetric detection device 100.

[0180] The control module 400 is installed in the colorimetric detection device 100 and connected to the image acquisition unit 13 and the automatic liquid addition module 20. The host computer 500 is connected to the control module 400 and can communicate with it.

[0181] In this embodiment, the control module 400 is integrated into the colorimetric detection device 100. Of course, it is not limited to this. For example, the control module 400 can also be integrated into the host computer 500.

[0182] In the illustrated embodiment, the control module 400 can be a control circuit board built based on a microprocessor, such as a microcontroller or digital signal processor, and the control module 400 is mounted on the outer cover side wall 15 connected to the support base plate 11. Additionally, the host computer 500 can refer to a personal computer, laptop computer, smart tablet, smartphone, etc.

[0183] The control module 400 can acquire the image information obtained by the image acquisition unit 13 and process the image information. It should be understood that after upgrading from manual to automatic liquid addition, the control module 400 also needs to be upgraded to control the automatic liquid addition module 20.

[0184] In specific applications, the control module 400 is connected to at least the multi-axis module 22, the X-axis linear module 25, and the hydraulic circuit assembly 24 to control the linear module to move the liquid injection needle 23 to the required position, and to control each pump and switching valve 244 in the hydraulic circuit assembly 24, thereby automating each step of the automatic liquid injection.

[0185] The control module 400 communicates with the host computer 500. Operators can input corresponding commands through the host computer 500 and the current status of the colorimetric detection device 100 can be displayed in real time.

[0186] Figure 12 is a flowchart illustrating the steps of a colorimetric detection control method according to one embodiment of this application. This colorimetric detection control method can be applied to the aforementioned colorimetric detection system, specifically deployed in the control module 400 and / or the host computer 500. The colorimetric detection control method may include the following steps:

[0187] S151, the detection card information of the detection card 30 is acquired through the image acquisition unit 13.

[0188] In one optional embodiment, the upper surface of the detection card 30 is provided with an identifier that can be acquired by the image acquisition unit 13 to obtain the detection card information corresponding to the detection card 30.

[0189] In this embodiment, a QR code is preferably used for identification. However, it is not limited to this; for example, a barcode or other similar tool could also be used. The information obtained from the identification on the test card also facilitates traceability.

[0190] It should be noted that the detection card information here may include the detection card model, the detection card liquid addition method, etc. In one optional embodiment, the detection card information can be acquired by the control module 400 and sent to the host computer 500.

[0191] In addition, the host computer 500 can display the test card information through a display device (such as a display screen). Operators can add samples to be tested to the sample station 241 according to the test card model and input the sample information into the host computer 500.

[0192] S152, based on the information on the detection card, the automatic liquid dispensing module 20 is controlled to operate the detection card 30, and the detection card 30 after liquid dispensing is completed is obtained.

[0193] In this embodiment, the host computer 500 can generate corresponding instructions based on the liquid addition method of the test card in the test card information. These instructions may include liquid extraction instructions, liquid injection instructions, cleaning instructions, sample temperature adjustment instructions, incubation time, etc.

[0194] The liquid extraction command includes information such as the desired extraction position of the injection needle 23, the operation command of the injection pump 2431, and the amount of sample to be extracted. It should be understood that determining the extraction position determines the type of sample to be extracted.

[0195] The injection command includes information such as the injection position to be moved by the injection needle 23, the operation command of the injection pump 2431, and the required movement position (first position or second position) of the corresponding detection card 30 in the card slot mechanism 12. It should be understood that determining the injection position determines the position of the detection card 30.

[0196] The cleaning command includes information such as the cleaning position to which the liquid injection needle 23 needs to be moved, the sequential action commands of the first cleaning pump 2433, the second cleaning pump 2434, the waste liquid pump 2432, and the switching valve 244.

[0197] It should be noted that the sequence of actions such as liquid extraction, liquid injection, and cleaning is also determined according to the liquid addition method of the test card. To facilitate understanding of this scheme, the first test card 31 is used as an example. As mentioned above, the first test card 31 has a gold cup orifice H2 and a sample orifice H3.

[0198] Specifically, by controlling the X-axis linear module 25, the first detection card 31 loaded at the card slot plate mechanism 12 is moved to the first position. Then, the multi-axis module 22 is controlled to move the liquid injection needle 23 so that the liquid injection pump 2431 can sample (draw liquid) 200 microliters at the sample station 241. After the sampling is completed, the multi-axis module 22 is controlled to move the liquid injection needle 23 above the gold cup hole H2 and drive the liquid injection needle 23 downward. The outer needle 234 punctures the membrane, the inner needle 233 moves downward, and the liquid injection pump 2431 controls the injection and mixing.

[0199] The multi-axis module 22 is controlled to move the liquid injection needle 23 to the cleaning station 242, and the first cleaning pump 2433, the second cleaning pump 2434, the waste liquid pump 2432 and the switching valve 244 are controlled to clean the liquid injection needle 23.

[0200] After the sample in the gold cup well H2 has been incubated for 3 minutes, after sampling is completed, the multi-axis module 22 continues to move the liquid injection needle 23 to the gold cup well H2 to draw 180 microliters of sample. The X-axis linear module 25 moves the first detection card 31 to the second position, controls the multi-axis module 22 to move the liquid injection needle 23 to the sample well H3, and injects the incubated sample into the sample well H3 to obtain the first detection card 31 after liquid injection is completed.

[0201] For example, the second detection card 32 is used as an example. It should be noted that the second detection card 32 only has sample hole H3.

[0202] After confirming that the sample station 241 has the corresponding sample, the sample is heated until it reaches 30°C. The second detection card 32, which is mounted on the card slot plate mechanism 12, is moved to the second position by controlling the X-axis linear module 25. Then, the multi-axis module 22 is controlled to move the liquid injection needle 23 so that the liquid injection pump 2431 can take 200 microliters of sample at the sample station 241. The multi-axis module 22 is then controlled to move the liquid injection needle 23 to the sample hole H3 and the liquid injection pump 2431 injects the sample into the sample hole H3.

[0203] The multi-axis module 22 is controlled to move the liquid injection needle 23 to the cleaning station 242, and the first cleaning pump 2433, the second cleaning pump 2434, the waste liquid pump 2432, and the switching valve 244 are controlled to clean the liquid injection needle 23. After waiting for 10 minutes of incubation, the second detection card 32 after liquid injection is completed is obtained.

[0204] It should be noted that the first test card 31 here can be, for example, a sulfonamide or chloramphenicol test card, and the second test card 32 can be a quinolone test card, a pH card, etc.

[0205] It should be noted that the above-mentioned instructions for automatic liquid addition can be directly generated from the detection card information obtained by the image acquisition unit 13, and / or can be input by the operator through the host computer 500 based on the detection card information. For example, the instruction to start heating the sample and the required heating temperature can be input and confirmed by the operator through the host computer 500. The type information of the detection card can be input and confirmed by the operator through the host computer 500. The relevant instructions for the action of the liquid addition needle 23 and the pump can be automatically generated.

[0206] S153, the image information of the detection card 30 after the liquid addition is completed is acquired by the image acquisition unit 13, and the detection result is obtained.

[0207] It should be understood that after the liquid is added, the test card 30 will change color or form a pattern. The image acquisition unit 13 can acquire the image information on the test card 30, and the host computer 500 can analyze the image information and obtain the test results.

[0208] It should be noted that in the case of manual liquid addition (where the automatic liquid addition module 20 is not available), only step S151 can be performed. The automatic liquid addition operation of the automatic liquid addition module 20 requires manual replacement.

[0209] In summary, the solution provided in this application has at least the following advantages:

[0210] 1. Enables high-throughput simultaneous detection of multiple detection cards (30 units per second), improving detection efficiency;

[0211] 2. It achieves the preservation of the original image of the detection card 30, including the traceability of the QR code and the original detection card results, thus realizing high-fidelity traceability;

[0212] 3. The acquisition of QR codes can include instructions for implementing detection methodologies, enabling automated acquisition and execution of automated detection methods and equipment;

[0213] 4. Card slot C is compatible with two different types of detection cards 30, enabling use in various scenarios;

[0214] 5. The automatic version can realize sample input and result output, and automatically realize the entire process of sample mixing, aspiration, incubation, sample addition, result acquisition and data analysis, reducing human error and intervention, improving the quality and objectivity of results, and improving efficiency;

[0215] 6. Miniaturization enables portable, mobile automated inspection, meeting the needs of various application scenarios;

[0216] 7. Modular design enables quick upgrades and production from manual to automatic versions, simplifying the production process.

[0217] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.

Claims

1. A colorimetric detection device, characterized in that, It includes a detection module (10) and an automatic liquid dispensing module (20), wherein the detection module (10) and the automatic liquid dispensing module (20) are configured to be detachably connected and cooperate; The detection module (10) includes: Support base plate (11); At least one card slot plate mechanism (12) is disposed on the supporting base plate (11) and has at least one card slot (C), the card slot (C) being used to load a detection card (30); and An image acquisition unit (13) is disposed above the card slot plate mechanism (12) to acquire image information of the detection card (30) in the card slot (C); The automatic liquid dispensing module (20) is used to transfer and dispense samples to the test card (30) in the card slot (C).

2. The colorimetric detection device according to claim 1, characterized in that, The slot plate mechanism (12) includes a slot plate (121) and at least one pressing push rod (122); The slot plate (121) has at least one slot (C), and each of the pressing push rods (122) is disposed on the slot plate (121) and corresponds to one slot (C). The pressing push rod (122) is configured to eject the detection card (30) along the extending direction of the slot (C) when a pushing force is applied toward the pressing push rod (122) via the detection card (30) in the slot (C).

3. The colorimetric detection device according to claim 2, characterized in that, The slot (C) extends along the X direction, and the top of the two side walls of the slot (C) in the Y direction are formed with steps that extend toward each other; The steps include a first stage (1211) and a second stage (1212); The second stage (1212) protrudes beyond the first stage (1211), and the lower sidewall of the second stage (1212) is higher than the lower sidewall of the first stage (1211).

4. The colorimetric detection device according to claim 3, characterized in that, The slot plate mechanism (12) also includes at least one spring piece (123); Each of the aforementioned spring pieces (123) is correspondingly disposed on the bottom side of one of the card slots (C) for cooperating with the spring pieces (123) to clamp the detection card (30) in the Z direction.

5. The colorimetric detection device according to any one of claims 1 to 4, characterized in that, The number of the card slot plate mechanisms (12) is multiple, and the multiple card slot plate mechanisms (12) are arranged side by side in the Y direction, and each card slot plate mechanism (12) is configured to load different types of detection cards (30); or The number of the card slot plate mechanism (12) is one and it is provided with multiple card slots (C) arranged side by side along the Y direction. The multiple card slots (C) are divided into multiple partitions arranged side by side in the Y direction. Each partition is configured to load different types of detection cards (30).

6. The colorimetric detection device according to claim 1, characterized in that, The automatic liquid dispensing module (20) includes: The splicing base plate (21) is detachably connected to the supporting base plate (11); A multi-axis module (22) is disposed on the splicing base plate (21); A liquid injection needle (23) is connected to the multi-axis module (22) and, driven by the multi-axis module (22), can switch between aspiration, injection, and cleaning positions; and The fluid assembly (24) is configured to draw liquid through the filling needle (23) at the drawing position, inject liquid through the filling needle (23) at the injection position, and clean the filling needle (23) at the cleaning position.

7. The colorimetric detection device according to claim 6, characterized in that, The liquid injection needle (23) includes a mounting block (231), an adjusting block (232), an inner needle (233), an outer needle (234), and an elastic element (235); The mounting block (231) is connected to the multi-axis module (22), and the outer pin (234) and the adjusting block (232) are respectively connected to the two Z-axis ends of the mounting block (231); The inner needle (233) passes through the adjusting block (232), the mounting block (231) and the outer needle (234) along the Z direction, and is able to move relative to the adjusting block (232), the mounting block (231) and the outer needle (234) in the Z direction. The end of the inner needle (233) away from the adjusting block (232) protrudes from the end of the outer needle (234) away from the adjusting block (232). The elastic element (235) is located between the adjusting block (232) and the mounting block (231). The adjusting block (232) is configured to adjust the mounting position in the Z direction to control the compression of the elastic element (235). The elastic element (235) is configured to be compressed when the inner needle (233) moves upward.

8. The colorimetric detection device according to claim 6, characterized in that, The fluid circuit assembly (24) includes a sample station (241), a cleaning station (242), a pump station (243), and a switching valve (244); The sample station (241) and the pump station (243) are both located on the splicing base plate (21), and the cleaning station (242) is located on the splicing base plate (21) or the supporting base plate (11); The pump station (243) is connected to the cleaning station (242), and the liquid injection needle (23) is connected to the pump station (243) through the switching valve (244). The switching valve (244) is used to switch the pump in the pump station (243) to which the liquid injection needle (23) is connected.

9. The colorimetric detection device according to claim 8, characterized in that, The pump station (243) includes a liquid filling pump (2431), a waste liquid pump (2432), a first cleaning pump (2433) and a second cleaning pump (2434); The liquid injection pump (2431) and the second cleaning pump (2434) are connected to the liquid injection needle (23) via the switching valve (244), and the first cleaning pump (2433) and the second cleaning pump (2434) are both connected to the cleaning station (242); At the liquid extraction position, the liquid injection needle (23) extends into the sample station (241) and extracts liquid through the liquid injection pump (2431); At the cleaning position, the liquid injection needle (23) extends into the cleaning station (242) and is cleaned by the first cleaning pump (2433) and the second cleaning pump (2434). At the injection position, the injection needle (23) extends into the detection card (30) in the card slot (C) and injects liquid through the injection pump (2431).

10. The colorimetric detection device according to claim 8, characterized in that, The sample station (241) includes a sample holder (2411), at least one heating plate (2412), a cup holder (2413), and multiple sample tubes (2414); The sample holder (2411) is connected to the splicing base plate (21) and is provided with a plurality of cup holder slots (D). Each cup holder slot (D) contains at least a portion of the cup holder (2413). At least one of the plurality of cup holder slots (D) contains a heating plate (2412). The heating plate (2412) is located between the bottom wall of the cup holder slot (D1) and the cup holder (2413). The cup holder (2413) is provided with a insertion hole (H1) for inserting the sample tube (2414).

11. The colorimetric detection device according to claim 6, characterized in that, The automatic liquid dispensing module (20) also includes at least one X-axis linear module (25); The X-axis linear module (25) is disposed on the support base plate (11) and connected to the card slot plate mechanism (12) for driving the card slot plate mechanism (12) to drive the detection card (30) to switch between the first position and the second position along the X direction.

12. The colorimetric detection device according to any one of claims 6 to 11, characterized in that, The multi-axis module (22) includes a Y-axis linear module (221) and a Z-axis linear module (222); The Z-axis linear module (222) is disposed on the splicing base plate (21), the Y-axis linear module (221) is connected to the Z-axis linear module (222) and is located above the splicing base plate (21), and the liquid injection needle (23) is connected to the Y-axis linear module (221); The Z-axis linear module (222) can drive the Y-axis linear module (221) to move the liquid injection needle (23) up and down along the Z direction, and the Y-axis linear module (221) can drive the liquid injection needle (23) to move along the Y direction.

13. A colorimetric detection system, characterized in that, It includes a host computer (500), a control module (400), and a colorimetric detection device (100) according to any one of claims 1 to 12; The control module (400) is located in the colorimetric detection device (100) and connected to the image acquisition unit (13) and the automatic liquid addition module (20). The host computer (500) is connected to the control module (400) and can communicate with it.

14. A colorimetric detection and control method, characterized in that, Applied to the colorimetric detection system as described in claim 13 and comprising: The detection card information of the detection card (30) is obtained through the image acquisition unit (13); The automatic liquid dispensing module (20) is controlled to operate the detection card (30) according to the detection card information, and the detection card (30) after liquid dispensing is completed is obtained; The image acquisition unit (13) acquires the image information of the detection card (30) after the liquid addition is completed, and obtains the detection result.

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