System for biochemical synthesis on surface of solid-phase chip

Abstract

A system for biochemical synthesis on the surface of a solid-phase chip. The system comprises: a closed cavity, which is internally provided with a marble platform (130) and a marble support (140), wherein a first horizontal direction driving device (200) can move a chip carrier device (300) carrying a solid-phase chip (811) on the marble platform (130), a second horizontal direction driving device (400) is mounted on the marble support (140) to control a printing device (520) to move horizontally in a direction perpendicular to a first horizontal direction to print on the surface of the solid phase chip (811); a moisture-retaining device (600), which is arranged on the marble platform (130), matches the printing device (520), and is configured for maintaining the state of the printing device (520); and a third direction driving device (700), which is located above the first horizontal direction driving device (200) and drives a microfluidic device (800) to move vertically, wherein when the microfluidic device (800) presses against the solid-phase chip (811) on the chip carrier device (300), a plurality of sealed reaction chambers (812) are formed for a DNA synthesis reaction. The whole system is compact in design and accurate in motion control, can efficiently perform biochemical synthesis, and maintains the stability of reaction environment.

Description

Solid-phase chip surface biochemical synthesis system Technical Field

[0001] This disclosure relates to the field of biochemical synthesis technology, and in particular to a solid-phase chip surface biochemical synthesis system. Background Technology

[0002] The most widely used and mature method for DNA synthesis is the phosphoramidite triester synthesis method, which includes four steps: deprotection, coupling, capping, and oxidation.

[0003] The first-generation column synthesizer disclosed in the prior art is based on the principle of phosphoramidite triester synthesis. Its synthesis carrier is a columnar tubular synthesis column, and the internally filled controlled porous glass (CPG) is the actual reaction medium. The highest single-batch synthesis throughput of the first-generation column synthesizer is only 1536 lines, the synthesis length is generally less than 150 nt, the yield is generally 0.5~100 nmol, the synthesis error rate is about ~1 / 1000 nt, and the cost is 0.05~0.5 yuan per nt. It is difficult to meet the new market demand for high-throughput synthesis, and it is also difficult to significantly reduce the synthesis cost. Summary of the Invention

[0004] In view of this, the present disclosure provides a solid-phase chip surface biochemical synthesis system, which at least partially solves the problems of low synthesis efficiency, poor precision, and inability to meet the needs of high-throughput synthesis in the prior art.

[0005] This disclosure provides a solid-phase chip surface biochemical synthesis system, including a sealed cavity, a marble platform disposed inside the sealed cavity, and a first horizontal driving device disposed on the marble platform;

[0006] The power output end of the first horizontal driving device is provided with a chip stage device, which is used to support solid-state chips.

[0007] The marble support set on the marble platform has a second horizontal direction driving device on its side that is perpendicular to the first horizontal direction; the power output end of the second horizontal direction driving device is provided with a printing device, which has a degree of freedom to move along the second horizontal direction, and is used to print on the surface of the solid-phase chip carried by the chip stage device.

[0008] A moisturizing device is installed on the marble platform and matched with the printing device, the moisturizing device being used for condition maintenance of the printing device;

[0009] A third-direction driving device is arranged across the top of the first horizontal driving device. A microfluidic device matching the chip stage device is installed at the power output end of the third-direction driving device. The microfluidic device has the freedom to move along the third direction. When the microfluidic device presses against the target chip on the chip stage device, it forms several sealed reaction chambers.

[0010] In some embodiments, the first horizontal driving device includes two first direction magnetic rails fixedly mounted parallel to the marble platform, a first stator winding fixedly installed with the marble platform, and a first mover assembly matched with the first stator winding. The first mover assembly includes a first linear motor mover and a first mover mounting block.

[0011] The chip stage device includes:

[0012] The stage body has a downward-facing chamber, and the top of the chamber has a chip mounting area with several through holes.

[0013] A platform support is disposed below the platform body; the platform support is connected to the platform body by a rotating pin; an angle adjustment component is provided on the platform support, and the platform body has the freedom to rotate horizontally around the center of the rotating pin under the adjustment of the angle adjustment component.

[0014] The stage support is provided with a sealing component that matches the chamber; a negative pressure adsorption component is installed below the sealing component, and the negative pressure adsorption component is connected to the chamber through a pipeline; the chamber forms a negative pressure environment facing several through holes under the action of the negative pressure adsorption component, so as to adsorb the chip covered on the chip mounting area.

[0015] A horizontal support plate is disposed at the bottom of the platform support; the bottom of the horizontal support plate has two sets of first engaging sliders that respectively match the two first direction magnetic rails;

[0016] The first linear motor actuator is fixed to the bottom of the horizontal support plate by the first actuator mounting block, and the first linear motor actuator is located between the two sets of the first engaging sliders.

[0017] In some embodiments, the second horizontal drive device includes two second direction magnetic rails parallel to and fixed to the side of the marble support, a second stator winding fixedly installed to the side of the marble support, and a second mover assembly matched with the second stator winding. The second mover assembly includes a second linear motor mover and a second mover mounting block.

[0018] The printing device engages with two second-direction magnetic rails via a vertical support plate. The side of the vertical support plate has two sets of second engaging sliders that respectively match the two second-direction magnetic rails. The second linear motor actuator is fixed to the bottom of the vertical support plate via a second actuator mounting block, and the second linear motor actuator is located between the two sets of second engaging sliders.

[0019] The printing device includes:

[0020] Nozzle assembly;

[0021] The visible ink cartridge has an independently configured individual ink loading chamber and an activator ink loading chamber inside. The individual ink loading chamber and the activator ink loading chamber are respectively connected to corresponding interfaces on the printhead device through pipelines. Both the visible ink cartridge and the printhead device are installed in the printing module housing.

[0022] A single ink filling pipeline device includes a first ink filling component, a first connector, and a second connector. One end of the first connector is connected to the single ink loading chamber, and the other end has a first engaging portion. One end of the second connector has a second engaging portion that matches the first engaging portion, and the other end is connected to the first ink filling component.

[0023] An activator ink filling pipeline device includes a second ink filling component, a third connector, and a fourth connector. One end of the third connector is connected to the activator ink loading chamber, and the other end has a third engaging portion. One end of the fourth connector has a fourth engaging portion that matches the third engaging portion, and the other end is connected to the second ink filling component. A one-way valve is installed in both the third engaging portion and the first engaging portion.

[0024] A pressure source device for providing negative pressure that matches the nozzle device.

[0025] In some embodiments, the nozzle device includes a plurality of independently configured single nozzles and a multi-nozzle position adjustment device for adjusting the positions of the plurality of single nozzles;

[0026] The multi-nozzle position adjustment device includes:

[0027] A nozzle support plate has a groove on it, and the groove has a plurality of nozzle mounting positions;

[0028] Each of the nozzle mounting positions is provided with a horizontal deflection adjustment component; the horizontal deflection adjustment component includes an elastic element disposed on the first side of the nozzle and an adjustment element disposed on the second side of the nozzle, and the line connecting the center of the adjustment element and the center of the elastic element is set at a preset angle with the longitudinal axis of the nozzle.

[0029] A horizontal movement adjustment assembly is installed on the nozzle support plate. The horizontal movement adjustment assembly includes a leveling screw and a spring plunger. A first hole for accommodating the leveling screw is opened on one side of the nozzle support plate, and a second hole for accommodating the spring plunger is opened on the other side. The second hole is matched with the first hole. The top of the leveling screw abuts against a third side of the nozzle, and the top of the spring plunger abuts against a fourth side of the nozzle. The leveling screw has the freedom to move along the first hole.

[0030] In some embodiments, the moisturizing device includes:

[0031] A drive assembly has a first slider mounted on its power output end, the first slider having a degree of freedom to move along the longitudinal axis of the power output end of the drive assembly; a second slider is fixed to the side of the first slider.

[0032] The transmission assembly includes a guide rail that engages with the second slider and a vertical plate for mounting the guide rail. The longitudinal axis of the guide rail is set at a preset angle with the moving direction of the first slider. The guide rail has the freedom to move up or down under the drive of the second slider.

[0033] A moisturizing ink pad assembly is fixedly installed on the top of the upright plate; the moisturizing ink pad assembly includes an ink-absorbing top plate, the ink-absorbing top plate has a groove, and a moisturizing pad matching the printhead is disposed in the groove;

[0034] The ink suction assembly includes an ink suction pump and a waste ink storage bottle connected to the ink suction pump. The ink suction pump is connected to the groove. When the printing device is in standby mode and is located directly above the groove, the groove forms a negative pressure environment facing the corresponding printhead under the action of the ink suction pump.

[0035] In some embodiments, the drive assembly includes a drive motor and a lead screw mounted on the power output end of the drive motor; the drive motor is mounted on a support plate, and the support plate is fixedly connected to the marble platform; the support plate is provided with a linear guide rail that engages with the bottom of the first slider, and the linear guide rail is arranged parallel to the lead screw;

[0036] The support plate is provided with a first side plate, a second side plate, a third side plate, a fourth side plate, and a bearing mounting plate. The first side plate, the second side plate, the third side plate, and the fourth side plate are all fixedly installed on the bottom of the bearing mounting plate. The first side plate is arranged adjacent to the second side plate and opposite to the third side plate. The drive motor is mounted on the support plate through the second side plate. The ink absorption assembly is installed on the outside of the fourth side plate.

[0037] The bottom of the ink-absorbing top plate is provided with several columns; the columns are fitted with shaft support seats that are fixedly installed to the bottom of the ink-absorbing top plate.

[0038] The top of the bearing mounting plate is equipped with a linear bearing component that matches the column, and the linear bearing component is matched with the shaft support seat.

[0039] In some embodiments, the third-party directional drive device includes a bearing device and a pressure plate lifting device mounted on the bearing member. The bearing device includes a support member and a bearing member mounted on the top of the support member. The bearing member and the support member are connected by a horizontal adjustment assembly, and the bearing member has a horizontal adjustment degree of freedom. The pressure plate lifting device includes a power component and a pressure plate assembly mounted on the power output end of the power component. The pressure plate assembly has a degree of freedom to move up and down along the longitudinal axis of the power output end of the power component.

[0040] The microfluidic device includes a fluid path system and a sealing channel device installed below the pressure plate assembly. The sealing channel device includes a partitioned channel body, a sealing ring, and a sealing pressure plate. The partitioned channel body has stepped protrusions on its side away from the pressure plate assembly, and the sealing ring is matched with the stepped protrusions. The sealing pressure plate is installed around the sealing ring and is fixedly connected to the partitioned channel body. The sealing ring has several sub-rings, and several of the sub-rings protrude from the sealing pressure plate. The protruding portions of the several sub-rings and the pressed solid-phase chip form several sealed reaction chambers.

[0041] The liquid circuit system includes a liquid supply unit, a gas supply unit, and a waste liquid collection unit. The liquid supply unit is connected to several of the sealed reaction chambers through a liquid supply pipeline assembly. The gas supply unit is connected to several of the sealed reaction chambers through a gas supply pipeline assembly. The waste liquid collection unit is connected to several of the sealed reaction chambers through a liquid extraction pipeline assembly.

[0042] In some embodiments, the microfluidic device further includes a gas-liquid tube lifting device fixedly connected to the pressure plate assembly;

[0043] The gas-liquid pipe lifting device includes a bridging plate, a gas source delivery pipe, a waste liquid collection pipe, a first hollow pipe accommodating the gas source delivery pipe, a second hollow pipe accommodating the waste liquid collection pipe, and a lifting drag chain.

[0044] The bridging plate is fixedly connected to the side of the pressure plate assembly;

[0045] Both the first hollow tube and the second hollow tube are fixedly connected to the bridge plate;

[0046] One end of the lifting cable chain is fixed to the side of the support member, and the other end is fixedly connected to the bridge plate.

[0047] In some embodiments, the solid-phase chip surface biochemical synthesis system further includes a synthesis chip information acquisition device installed on the side of the marble support. The synthesis chip information acquisition device includes a dual-camera adjustment assembly and two cameras installed on the dual-camera adjustment assembly. The field of view centers of the two cameras are respectively aligned with the centers of the two crosshairs of the solid-phase chip.

[0048] The dual-camera adjustment assembly includes:

[0049] An assembly support plate is provided with a Z-axis displacement stage on its side. A turntable is provided on the side of the Z-axis displacement stage away from the assembly support plate, and the side of the Z-axis displacement stage away from the assembly support plate has a degree of freedom of movement parallel to the Z-axis direction.

[0050] The turntable is equipped with a transition plate on the side away from the Z-axis displacement stage, and the side of the turntable away from the Z-axis displacement stage has a degree of freedom to rotate around the X-axis.

[0051] The adapter plate is fixedly connected to the side of the turntable by several support rods, and the connection between the several support rods and the adapter plate has a degree of freedom of movement parallel to the Y-axis.

[0052] The adapter plate and the turntable form an accommodating space; the accommodating space is provided with a vertical support plate, the vertical support plate has a camera mounting part, and the vertical support plate is connected to the adapter plate through a plurality of joint bearing units, the plurality of joint bearing units having a degree of freedom to drive the vertical support plate to rotate around the Z-axis, a degree of freedom to rotate around the Y-axis, and a degree of freedom to move along a direction parallel to the X-axis.

[0053] In some embodiments, the solid-phase chip surface biochemical synthesis system further includes a multi-nozzle flexible ink wiping device installed on the marble platform, the multi-nozzle flexible ink wiping device being used to simultaneously wipe multiple independently configured single nozzles in the printing device.

[0054] The multi-nozzle flexible ink-wiping device includes:

[0055] A first load-bearing platform fixedly connected to the marble platform;

[0056] The first drive component is installed on the first carrier platform;

[0057] A slider assembly is installed at the power output end of the first drive assembly; the slider assembly has a degree of freedom to move along the longitudinal axis of the first bearing platform;

[0058] The slider assembly is equipped with a soiled nonwoven fabric tightening assembly, a new nonwoven fabric supply assembly, and an ink wiping assembly. A guide shaft is provided between the soiled nonwoven fabric tightening assembly and the ink wiping assembly to stretch the nonwoven fabric outward. A tensioning shaft is provided between the ink wiping assembly and the new nonwoven fabric supply assembly to press the nonwoven fabric inward. The center line connecting the guide shaft and the tensioning shaft is parallel to the center line connecting the soiled nonwoven fabric tightening assembly and the new nonwoven fabric supply assembly, and the center line connecting the guide shaft and the tensioning shaft is set lower than the ink wiping assembly.

[0059] The ink wiping assembly stretches the non-woven fabric outward to form a flexible ink wiping area that matches the multiple printheads;

[0060] The distance from the top of the flexible ink-wiping area to the marble platform is h1, and the distance from the bottom of the printing device to the marble platform is h2, where 3mm ≤ h1 - h2 ≤ 5mm.

[0061] The solid-phase chip surface biochemical synthesis system provided in this embodiment adopts a closed-cavity design, which can provide a completely sealed environment, ensuring an inert gas environment inside and effectively isolating water and oxygen. This helps prevent DNA from being oxidized or degraded during synthesis, improving the success rate and efficiency of synthesis. The system includes first, second, and third-axis driving devices, which can achieve high-precision horizontal and vertical motion control, ensuring that the printing device and microfluidic device can operate accurately on the chip stage. This multi-axis motion control ensures that every position on the chip can be precisely processed. The printing device is used to print on the solid-phase chip surface. Combined with the sealed reaction chamber formed by the microfluidic device, high-throughput DNA synthesis can be achieved. Through the clamping operation of the microfluidic device, multiple independent reaction chambers can be formed on the chip stage, thereby performing multiple DNA synthesis reactions simultaneously and improving synthesis efficiency. The presence of a humidification device ensures that the printing device remains in a suitable state during operation, extending the service life of the equipment. At the same time, the closed cavity is designed with multiple operating ports, which facilitates the maintenance and monitoring of the equipment by the operator, improving the convenience and efficiency of operation.

[0062] The above description is merely an overview of the technical solution disclosed herein. In order to better understand the technical means of this disclosure and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this disclosure more apparent and understandable, preferred embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description

[0063] To more clearly illustrate the technical solutions of the embodiments of this disclosure, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this disclosure. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0064] Figure 1 is a three-dimensional schematic diagram of a specific embodiment of the solid-phase chip surface biochemical synthesis system provided in this application.

[0065] Figure 2 is a schematic diagram of the assembly of the device installed inside the sealed cavity in Figure 1.

[0066] Figure 3 is a three-dimensional schematic diagram of the first horizontal drive device in Figure 2.

[0067] Figure 4 is a schematic diagram of the assembly of some components in the chip stage device in Figure 3.

[0068] Figure 5 is an exploded view of Figure 4.

[0069] Figure 6 is a schematic diagram of Figure 5 from another angle.

[0070] Figure 7 is a schematic diagram of a first embodiment of the platform support in this application.

[0071] Figure 8 is a schematic diagram of a second embodiment of the platform support in this application.

[0072] Figure 9 is a schematic diagram of the horizontal support plate in the chip stage device.

[0073] Figure 10 is a schematic diagram of the assembly of the second horizontal direction drive device and the printing device in Figure 2.

[0074] Figure 11 is an exploded view of some components of the printing device in Figure 10.

[0075] Figure 12 is a schematic diagram of ink supply for a single printhead in Figure 11.

[0076] Figure 13 is a schematic diagram of the explosion of the visible ink cartridge in Figure 12.

[0077] Figure 14 is a three-dimensional schematic diagram of the box in Figure 13.

[0078] Figure 15 is a schematic diagram of the installation of multiple single nozzles.

[0079] Figure 16 is a three-dimensional schematic diagram of the multi-nozzle position adjustment device in Figure 15.

[0080] Figure 17 is a schematic diagram of Figure 15 from another angle.

[0081] Figure 18 is a three-dimensional schematic diagram of the moisturizing device in Figure 2.

[0082] Figure 19 is a schematic diagram of the explosion in Figure 18.

[0083] Figure 20 is a schematic diagram of the assembly of the moisturizing ink pad assembly and the transmission assembly in Figure 19.

[0084] Figure 21 is a schematic diagram of the assembly of the transmission component and the second slider in Figure 20.

[0085] Figure 22 is a schematic diagram of the assembly of the third-party drive device and the microfluidic device in Figure 2.

[0086] Figure 23 is a three-dimensional schematic diagram of the support device in Figure 22.

[0087] Figure 24 is a three-dimensional schematic diagram of the pressure plate lifting device in Figure 22.

[0088] Figure 25 is a three-dimensional schematic diagram of the sealing flow channel device in Figure 22.

[0089] Figure 26 is an exploded view of some components of the sealing flow channel device in Figure 22.

[0090] Figure 27 is a right perspective view of the sealing flow channel device in Figure 22.

[0091] Figure 28 is an exploded schematic diagram of the sealing flow channel device in Figure 22.

[0092] Figure 29 is a magnified view of part A in Figure 28.

[0093] Figure 30 is a schematic diagram of the assembly of the gas-liquid pipe lifting device in Figure 22.

[0094] Figure 31 is a schematic diagram of the installation of the synthetic chip information acquisition device.

[0095] Figure 32 is a three-dimensional schematic diagram of the dual-camera adjustment component in Figure 31 during use.

[0096] Figure 33 is a schematic diagram of the assembly of the Z-axis displacement stage and the turntable in Figure 32.

[0097] Figure 34 is a schematic diagram of the installation of the spherical bearing unit in Figure 32.

[0098] Figure 35 is a three-dimensional schematic diagram of the multi-nozzle flexible ink-wiping device in Figure 2.

[0099] Figure 36 is a schematic diagram of the explosion in Figure 35.

[0100] Figure 37 is a magnified view of part B in Figure 36.

[0101] Explanation of reference numerals in the attached figures:

[0102] 110. Steel frame; 120. Glove box; 130. Marble platform; 140. Marble support;

[0103] 200. First horizontal direction drive device;

[0104] 300. Chip stage device; 310. Stage body; 301. Chamber; 320. Stage support; 321. First support frame; 322. First flat plate; 323. First branch; 324. Second support frame; 325. Second flat plate; 326. Second branch; 330. Hollow bearing plate; 340. Bearing bracket; 351. Sealing plate; 352. Sealing gasket; 360. Angle adjustment assembly; 361. First angle adjustment plate; 362. Second angle adjustment plate; 370. Rotating pin; 380. Negative pressure adsorption assembly; 390. Horizontal support plate;

[0105] 400. Second horizontal direction drive device;

[0106] 510. Vertical support plate; 520. Printing device; 530. Visual ink cartridge; 531. Cartridge body; 532. Individual ink loading chamber; 533. Activator ink loading chamber; 5341. First annular groove; 5342. Second annular groove; 5343. First ink outlet; 5344. Second ink outlet; 5345. First adapter; 5346. Second adapter; 535. Cover plate; 536. First through hole; 537. Second through hole; 540. Individual ink filling pipeline device; 541. First connector; 542. Second connector; 543. First ink filling assembly; 550. Activator ink filling pipeline device; 551. Third connector; 552. Fourth connector; 553. Second ink filling assembly; 561. First pipeline; 562. First filter; 56 3. Second conduit; 564. Second filter; 570. Overflow ink cartridge; 580. Pressure source device; 590. Printhead assembly; 591. Single printhead; 592. Printhead support plate; 5921. First groove; 5922. Through hole; 593. Positioning hole; 5941. Protrusion; 5942. First edge; 5951. First adjustment through hole; 5952. Second adjustment through hole; 5961. Second edge; 5962. Third adjustment through hole; 5963. Fourth adjustment through hole; 5971. Elastic element; 5972. Adjusting element; 5981. First hole; 5982. Second hole; 5983. Spring plunger; 5991. First adjusting screw; 5992. Second adjusting screw; 5993. Third adjusting screw; 5994. Fourth adjusting screw;

[0107] 600. Humidifying device; 610. Drive assembly; 611. Drive motor; 612. Lead screw; 621. First slider; 622. Second slider; 630. Transmission assembly; 631. Guide rail; 632. Vertical plate; 633. Limiting sensor; 640. Humidifying ink pad assembly; 641. Ink-absorbing top plate; 642. Second groove; 643. Humidifying pad; 644. Column; 645. Shaft support seat; 646. Sheet metal upper cover; 650. Ink-absorbing assembly; 661. Bearing plate; 662. Linear guide rail; 663. First side plate; 664. Second side plate; 665. Third side plate; 666. Fourth side plate; 667. First bearing mounting plate; 6671. Linear bearing component; 668. Slider limiting component; 669. Motor protection block; 671. First sheet metal cover; 672. Second sheet metal cover;

[0108] 700. Third-party drive unit;

[0109] 800. Microfluidic device; 811. Chip; 812. Sealed reaction chamber; 820. Support device; 821. Bracket; 8211. Gantry base plate; 8212. Gantry vertical plate; 822. Horizontal plate; 8221. Motor mounting hole; 8222. Column mounting hole; 823. Spherical bearing; 824. Second bearing mounting plate; 825. Decorative plate; 830. Pressure plate lifting device; 831. Servo motor; 832. Reducer; 833. T-type lead screw nut; 834. T-type lead screw; 835. Bearing assembly; 836. Pressure equalizing plate; 837. Ball spline; 838. Locking cover; 840. Sealed flow Channel device; 841, partitioned flow channel body; 842, stepped protrusion; 843, first layer protrusion; 844, second layer protrusion; 845, first contact part; 846, second contact part; 850, sealing ring; 851, sub-ring; 852, first sealing part; 853, second sealing part; 860, sealing pressure plate; 861, first pressure plate; 862, second pressure plate; 863, third pressure plate; 864, fourth pressure plate; 870, liquid inlet; 880, liquid outlet; 890, gas-liquid pipe lifting device; 891, bridging plate; 892, first hollow pipe; 893, second hollow pipe; 894, lifting drag chain;

[0110] 900. Synthetic chip information acquisition device; 911. Camera; 912. Light source assembly; 920. Assembly support plate; 930. Z-axis displacement stage; 931. First plate; 932. Second plate; 933. Adjustment knob; 934. Locking component; 940. Turntable; 950. Adapter plate; 960. Support rod; 961. First rod; 962. Second rod; 963. Third rod; 964. Fourth rod; 970. Vertical bearing plate; 971. Body mounting plate; 972. Lens limiting plate; 980. Joint bearing unit; 981. First unit; 982. Second unit; 983. Third unit;

[0111] 1000. Multi-nozzle flexible ink erasing device; 1100. First support platform; 1110. Guide rail; 1200. First drive assembly; 1210. First motor; 1220. First lead screw; 1230. Motor mounting bracket; 1300. Slider assembly; 1310. Second support platform; 1320. Transition plate; 1330. Vertical support plate; 1400. Dirty nonwoven fabric tightening assembly; 1410. Winding motor; 1 420, Drive shaft; 1430, Dirty nonwoven fabric roll; 1500, New nonwoven fabric supply assembly; 1510, Damping device; 1520, Driven shaft; 1530, New nonwoven fabric roll; 1600, Ink erasing assembly; 1610, Overhang plate; 1621, First long rubber pad; 1622, Second long rubber pad; 1700, Guide shaft; 1800, Tensioning shaft; 1910, First sensor; 1920, Second sensor. Detailed Implementation

[0112] The embodiments of this disclosure will now be described in detail with reference to the accompanying drawings.

[0113] It should be understood that the following specific examples illustrate the implementation of this disclosure, and those skilled in the art can easily understand other advantages and effects of this disclosure from the content disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of this disclosure, and not all of them. This disclosure can also be implemented or applied through other different specific implementation methods, and the details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this disclosure. It should be noted that, in the absence of conflict, the following embodiments and features in the embodiments can be combined with each other. Based on the embodiments in this disclosure, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this disclosure.

[0114] Referring to Figures 1 and 2, this application discloses a solid-phase chip surface biochemical synthesis system, including a sealed cavity. In this embodiment, the sealed cavity refers to the glove box 120, which is supported by a steel frame 110.

[0115] The sealed cavity is equipped with a marble platform 130, and a first horizontal drive device 200 is installed on the marble platform; a chip stage device 300 is installed at the power output end of the first horizontal drive device, and the chip stage device is used to support solid phase chips.

[0116] A marble support 140 is installed on a marble platform, and a second horizontal direction drive device 400 is provided on its side, which is perpendicular to the first horizontal direction and is located above the first horizontal direction drive device.

[0117] The power output end of the second horizontal drive device is provided with a printing device 520. The printing device has the degree of freedom to move along the second horizontal direction and is used to print on the surface of the solid chip carried by the chip stage device 300.

[0118] A humidification device 600 is installed on the marble platform 130 and matched with the printing device 520. The humidification device is used to perform condition maintenance on the printing device.

[0119] A third-direction drive device 700 is arranged horizontally above the first horizontal drive device. A microfluidic device 800 matching the chip stage device is installed at the power output end of the third-direction drive device. The microfluidic device has the freedom to move along the third direction. When the microfluidic device presses against the target chip on the chip stage device, it forms several sealed reaction chambers.

[0120] In this embodiment, all components operate within a sealed cavity. The cavity is completely sealed, providing an inert gas environment that isolates water and oxygen. Items can be transferred via a transition compartment (not shown in the figure) located on the side. The sealed cavity has five operating ports on the front, two on the right side, and two on the back for easy operation.

[0121] The solid-phase chip surface biochemical synthesis system disclosed in this application adopts a closed-cavity design, which can provide a completely sealed environment, ensuring an inert gas environment inside and effectively isolating water and oxygen. This helps prevent DNA from being oxidized or degraded during synthesis, improving the success rate and efficiency of synthesis. The system includes first, second, and third-axis driving devices, which can achieve high-precision horizontal and vertical motion control, ensuring that the printing device and microfluidic device can operate accurately on the chip stage. This multi-axis motion control ensures that every position on the chip can be precisely processed. The printing device is used to print on the solid-phase chip surface. Combined with the sealed reaction chamber formed by the microfluidic device, high-throughput DNA synthesis can be achieved. Through the clamping operation of the microfluidic device, multiple independent reaction chambers can be formed on the chip stage, thereby performing multiple DNA synthesis reactions simultaneously and improving synthesis efficiency. The presence of a humidification device ensures that the printing device remains in a suitable state during operation, extending the service life of the equipment. At the same time, the closed cavity is designed with multiple operating ports, which facilitates the maintenance and monitoring of the equipment by the operator, improving the convenience and efficiency of operation.

[0122] The system uses a side-mounted transition chamber for material transfer, ensuring that the internal environment of the sealed cavity remains unaffected by external factors during transfer, further guaranteeing operational safety and environmental stability. In summary, this solid-phase chip surface biochemical synthesis system significantly improves the efficiency and success rate of DNA synthesis through its design in multiple aspects, including a sealed cavity, high-precision motion control, high-throughput synthesis, ease of equipment maintenance, and operational convenience, while simultaneously ensuring operational safety and environmental stability.

[0123] Referring to Figures 3, 4 and 9, the first horizontal drive device includes two first direction magnetic rails fixedly mounted parallel to the marble platform, a first stator winding fixedly installed with the marble platform, and a first mover assembly matched with the first stator winding. The first mover assembly includes a first linear motor mover and a first mover mounting block.

[0124] The chip stage device includes: a stage body 310 and a stage support 320 disposed below the stage body 310. The stage support 320 is provided with a sealing component that matches the chamber 301. Below the sealing component, a negative pressure adsorption component 380 for providing negative pressure is installed. A horizontal support plate 390 is provided at the bottom of the stage support 320. The bottom of the horizontal support plate 390 has two sets of first engaging sliders that match two first direction magnetic tracks respectively. A first linear motor mover is fixed to the bottom of the horizontal support plate 390 through a first mover mounting block, and the first linear motor mover is located between the two sets of first engaging sliders.

[0125] The first horizontal drive device is a U-shaped groove linear motor. The driving principle of the U-shaped groove linear motor is based on the principle of electromagnetic induction and the principle of Lorentz force. When alternating current is applied to the first stator winding, a traveling wave magnetic field is generated. The conductor in the motor mover cuts the magnetic field lines in this magnetic field, generating an induced electromotive force, which in turn forms an induced current. The induced current interacts with the traveling wave magnetic field to generate an electromagnetic thrust along the straight line, which drives the motor mover to move in a straight line. That is, the chip carrier device moves along the first horizontal direction through the first mover mounting block.

[0126] Specifically, the stage body 310 has a downward-facing chamber 301, the top of which has a chip mounting area with several through holes.

[0127] The stage support 320 is connected to the stage body 310 via a rotating pin 370. An angle adjustment component 360 is provided on the stage support 320. Under the adjustment of the angle adjustment component 360, the stage body 310 has the freedom to rotate horizontally around the center of the rotating pin 370, so that the angle of the chip can be flexibly adjusted to adapt to different experimental needs, thereby improving the flexibility and versatility of the device.

[0128] The design of the stage body 310 and stage support 320 ensures that the stage surface has sufficient flatness and precision, which can be adapted to high-throughput synthesis chips that can withstand large pressure. It can effectively avoid the problem of chips being crushed due to uneven pressure during the pressing process, and ensure the integrity of the chips and the success rate of the experiment.

[0129] Referring to Figures 5 and 6, the negative pressure adsorption component 380 is connected to the chamber 301 through a pipeline; under the action of the negative pressure adsorption component 380, the chamber 301 forms a negative pressure environment facing several through holes to adsorb the chip covered on the chip mounting area.

[0130] Specifically, when the chip covers the upper surface of the chip mounting area, the cavity 301 under the lower sealing component forms a sealed cavity 301. Therefore, the negative pressure provided to the sealed cavity 301 by the negative pressure adsorption component 380 can be applied to the chip through several through holes, thereby firmly adsorbing the chip onto the upper surface of the chip mounting area and achieving effective fixation of the chip.

[0131] The negative pressure environment provided by the negative pressure adsorption component 380 allows the sealed chamber 301 to maintain better sealing, preventing leakage of highly corrosive reagents, protecting the integrity of equipment components, and improving the service life and safety of the equipment.

[0132] In this embodiment, a plurality of through holes are preferably arranged in an array to form a uniform adsorption force on the chip, thereby achieving uniform negative pressure adsorption on the chip without damaging it. This ensures the accurate landing position of the inkjet printing ink and effectively reduces reaction failure or contamination problems caused by positional deviation.

[0133] Furthermore, the chip mounting area has a positioning edge on its periphery. Through tolerance control, after the chip is placed within the positioning edge, it can be limited to the calibration range allowed by the alignment camera. The stage body 310 is preferably made of 316L stainless steel, which is high in strength and corrosion resistant, ensuring that the chip can maintain its shape stability and not deform when subjected to pressure, thus ensuring a high degree of flatness.

[0134] The sealing assembly includes a sealing plate 351 and a sealing gasket 352 disposed on the sealing plate 351. The periphery of the sealing gasket 352 is abutted against the periphery of the chamber 301 for sealing the chamber 301 from below in the assembled state.

[0135] The sealing plate 351 has several through holes, all of which are inside the hollow part of the sealing gasket 352.

[0136] Referring to Figures 5 and 6, the negative pressure adsorption assembly 380 includes a power component, an air collection manifold connected to the power output end of the power component, and several air pipes, which are independently arranged. One end of each air pipe is connected to a different outlet of the air collection manifold, and the other end extends through several through holes to the chamber 301. The number of air pipes is consistent with the number of through holes, which satisfies both the negative pressure function and the sealing effect on the chamber 301.

[0137] The power components include a vacuum pump and a vacuum breaker valve. One end of the vacuum breaker valve is connected to the vacuum pump, and the other end is open to the environment. When it is necessary to fix the chip, i.e. when negative pressure is required, the vacuum pump starts, and the vacuum breaker valve is connected to the vacuum pump. The vacuum pump starts to extract air from the sealed chamber 301 through the gas collector and several gas pipes to form a negative pressure environment. At this time, the vacuum breaker valve is connected to the vacuum pump to ensure that the vacuum pump can effectively extract gas and maintain negative pressure in the chamber 301.

[0138] During vacuum pump operation, the vacuum breaker valve remains connected to the vacuum pump to prevent external air from entering chamber 301 and maintain a negative pressure state within chamber 301. The negative pressure environment acts on the chip covered on the chip mounting area through several through holes, making it firmly adsorbed on the stage and ensuring the stability of the chip position during DNA biosynthesis.

[0139] When it is necessary to break the negative pressure environment, the vacuum pump stops working, and the vacuum breaker valve switches to the state of being in communication with the environment, allowing external air to enter the chamber 301, quickly neutralizing the negative pressure in the chamber 301, and releasing the chip from the adsorption state. This process can be achieved by controlling the opening and closing state of the vacuum breaker valve, which is simple and quick to operate.

[0140] Referring to Figure 7, in the first embodiment, the platform support 320 includes a first support frame 321 and a second support frame 324. The first support frame 321 includes a first flat plate portion 322 and a first branch portion 323 located below the first flat plate portion 322. The first flat plate portion 322 has a first pin hole that matches the rotating pin 370. The platform body 310 has a second pin hole that matches the first pin hole, ensuring that after the platform body 310 and the platform support 320 are assembled, the second pin hole is aligned with the first pin hole.

[0141] The second support frame 324 includes a second flat plate portion 325 and a second branch portion 326 located below the second flat plate portion 325; one side of the sealing plate 351 is located on the first flat plate portion 322, and the other side is located on the second flat plate portion 325.

[0142] Specifically, the length between one side of the sealing plate 351 on the first plate portion 322 and the other side of the sealing plate 351 on the second plate portion 325 is H1, the distance between the inner side of the first plate portion 322 and the inner side of the second plate portion 325 is H2, and the distance between the outer side of the first plate portion 322 and the outer side of the second plate portion 325 is H3, where H2 < H1 < H3, ensuring that the independently installed first support frame 321 and second support frame 324 have good load-bearing stability.

[0143] The angle adjustment assembly 360 includes a first angle adjustment plate 361 and a second angle adjustment plate 362. The first angle adjustment plate 361 is installed on the first side of the second flat plate portion 325, and the second angle adjustment plate 362 is installed on the second side of the second flat plate portion 325. The first angle adjustment plate 361 has a first adjustment hole for accommodating a first set screw, and the top end of the first set screw abuts against one side of the platform body 310.

[0144] The second angle adjustment plate 362 has a second adjustment hole for accommodating the second set screw, and the second adjustment hole is corresponding to the first adjustment hole; the top of the second set screw is pressed against the other side of the platform body 310.

[0145] When assembled, the stability of the platform body 310 can be ensured by the first set screw that abuts against one side of the platform body 310 and the second set screw that abuts against the other side of the platform body 310.

[0146] In this embodiment, the first set screw has the degree of freedom to move along the first adjustment hole; the second set screw has the degree of freedom to move along the second adjustment hole. Specifically, when the position of the chip needs to be adjusted, that is, when the position of the stage body 310 needs to be adjusted, for example, when clockwise adjustment is required, the first set screw is controlled to move outward (i.e., move away from the stage body 310), and the second set screw is controlled to move inward (i.e. move closer to the stage body 310). Under the thrust of the second set screw, the stage body 310 will rotate clockwise around the center of the rotating pin 370. When the horizontal rotation is in place, the top ends of the first set screw and the second set screw are respectively pressed against the two sides of the stage body 310.

[0147] When counterclockwise adjustment is required, the second setter screw is controlled to move outward (i.e., move away from the main body 310 of the platform) and the first setter screw is controlled to move inward (i.e. move closer to the main body 310 of the platform). Under the thrust of the first setter screw, the main body 310 of the platform will rotate counterclockwise around the center of the rotating pin 370. When the horizontal rotation is in place, the tops of the first setter screw and the second setter screw will abut against the two sides of the main body 310 of the platform, respectively.

[0148] In this embodiment, the second angle adjustment plate 362 has the same structure as the first angle adjustment plate 361, which facilitates processing.

[0149] The distance from the rotating pin 370 to the inner side of the first angle adjusting plate 361 is equal to the distance from the rotating pin 370 to the inner side of the second angle adjusting plate 362, ensuring precise control of the rotation angle.

[0150] Referring to Figure 8, in the second embodiment, the platform support 320 includes a hollow support plate 330 and a support bracket 340 fixed below the hollow support plate 330. The hollow support plate 330 has a through hole, the area of ​​which is smaller than the area of ​​the sealing plate 351. The hollow support plate 330 has a first pin hole that matches the rotating pin 370, and the platform body 310 has a second pin hole that matches the first pin hole.

[0151] The angle adjustment assembly 360 includes a first angle adjustment plate 361 and a second angle adjustment plate 362 respectively installed on the first side and the second side of the hollow support plate 330; the distance from the rotating pin 370 to the inner side of the first angle adjustment plate 361 is equal to the distance from the rotating pin 370 to the inner side of the second angle adjustment plate 362.

[0152] The first angle adjustment plate 361 has a first adjustment hole for accommodating the first set screw, and the top end of the first set screw abuts against one side of the stage body 310; the second angle adjustment plate 362 has a second adjustment hole for accommodating the second set screw, and the second adjustment hole is corresponding to the first adjustment hole; the top end of the second set screw abuts against the other side of the stage body 310.

[0153] The first set screw has a degree of freedom to move along the first adjustment hole; the second set screw has a degree of freedom to move along the second adjustment hole.

[0154] Furthermore, the chip mounting area has a retaining edge on its periphery, which forms a groove with the chip mounting area to store leaked reagents.

[0155] The chip stage device also includes a waste discharge component, which includes a negative pressure pump and a storage bottle. The negative pressure pump is connected to the groove through a pipeline, and the waste liquid in the groove can be drawn out to the storage bottle through the pipeline under the negative pressure of the negative pressure pump.

[0156] Furthermore, a liquid detection sensor is installed at the end of the pipe located in the groove to detect liquid, and an alarm is triggered when liquid is detected.

[0157] The chip platform device also includes a central control center. The negative pressure pump and liquid detection sensor are all connected to the central control center. The central control center controls the negative pressure pump to start pumping away waste liquid based on the alarm information of the liquid detection sensor. That is, when the liquid detection sensor detects liquid, it triggers an alarm and then immediately starts the negative pressure pump to pump away the liquid.

[0158] In this embodiment, all parts in contact with liquid are made of materials resistant to corrosion by all reagents, thereby improving the service life of the device.

[0159] The chip stage device disclosed in this application creates a negative pressure environment within the chamber through a negative pressure adsorption component. This effectively adsorbs the chip covering the chip mounting area, ensuring the chip remains stable during DNA biosynthesis and avoiding experimental errors caused by chip movement or vibration. The stage body and stage support are connected by a rotating pin and equipped with an angle adjustment component, allowing the stage body to rotate horizontally around the center of the rotating pin. This design allows the chip to be adjusted to the optimal angle to optimize the DNA synthesis process and adapt to different experimental needs. The stage support is equipped with a sealing component that matches the chamber, ensuring the chamber's airtightness and preventing external contamination. The entry of substances into the chamber affects the purity and accuracy of DNA synthesis, while ensuring the stability of negative pressure adsorption and maintaining the chip's position. The stage support is connected to the main body of the stage via a simple and stable rotating pin. The angle adjustment component is rationally designed and easy to operate. This structure not only improves the practicality of the device but also facilitates user adjustments and maintenance. Through angle adjustment, the chip can be repositioned on the stage with precise relative positioning. Furthermore, this design takes into account chips of different sizes and types. By adjusting the geometry of the chamber and the distribution of through holes, it can adapt to the needs of various biochips, further enhancing the device's versatility and practicality.

[0160] This chip stage device allows for precise chip positioning through position adjustment, ensuring accurate camera alignment. Negative pressure adsorption provides reliable chip fixation, guaranteeing precise inkjet ink placement and allowing alkaline ink to repeatedly land on physical reaction sites, thus ensuring optimal reaction results.

[0161] After the coupling reaction is completed by inkjet printing, it is generally completed by a fluid device. The fluid device is usually a pressing mechanism that presses against the chip surface to form a sealed reaction chamber. Different reagents are then introduced into the reaction chamber to carry out reactions such as capping, oxidation, and deprotection. During these reactions, the chip stage device can effectively seal the chip to ensure that the reagents do not leak.

[0162] In addition, the chip stage device can provide excellent flatness, ensuring that the chip will not be damaged even under great pressure during pressing, effectively guaranteeing the accuracy and reliability of DNA chip synthesis.

[0163] Referring to Figures 3 and 10, the second horizontal drive device 400 includes two second direction magnetic rails fixedly and parallel to the side of the marble support, a second stator winding fixedly installed with the side of the marble support, and a second mover assembly matched with the second stator winding. The second mover assembly includes a second linear motor mover and a second mover mounting block.

[0164] The printing device engages with two second-direction magnetic rails via a vertical support plate 510. The side of the vertical support plate 510 has two sets of second engagement sliders that match the two second-direction magnetic rails respectively. The second linear motor mover is fixed to the bottom of the vertical support plate via a second mover mounting block, and the second linear motor mover is located between the two sets of second engagement sliders.

[0165] The second horizontal drive device is a U-shaped groove linear motor. The driving principle of the U-shaped groove linear motor is based on the principles of electromagnetic induction and Lorentz force. When alternating current is applied to the second stator winding, a traveling wave magnetic field is generated. The conductor in the motor mover cuts the magnetic field lines in this magnetic field, generating an induced electromotive force, which in turn forms an induced current. The induced current interacts with the traveling wave magnetic field to generate an electromagnetic thrust along the straight line, which drives the motor mover to move in a straight line. That is, the vertical support plate 510 and the printing device 520 are moved along the second horizontal direction through the second mover mounting block, which can perform reciprocating linear motion and execute the printing action.

[0166] Referring to Figures 10 and 11, in this embodiment, the printing device has 5 sets of printheads arranged in a straight line; corresponding to 5 sets of ink path negative pressure systems, 5 printhead driver boards, and 5 buffer ink overflow bottles. The printing device can perform printing actions, each printhead is individually controllable, and can print individually or simultaneously, with the ejected ink droplets falling at any designated location.

[0167] The printing device includes: an overflow ink cartridge 570, a visible ink cartridge 530 installed in the printing module housing, and a printhead device 590. There are 5 sets of visible ink cartridges 530, which are arranged side by side and fixed to the printing device by an ink cartridge carrier plate. There are also 5 sets of overflow ink cartridges 570 corresponding to the visible ink cartridges 530.

[0168] Referring to Figures 12 and 13, the printing device will be further described in detail below using a single printhead ink supply printing system as an example. In a single printhead system, the printing device also includes: a single ink filling pipeline device 540, an activator ink filling pipeline device 550, and a pressure source device 580. The visible ink cartridge 530 has an independently configured single ink loading chamber 532 and an activator ink loading chamber 533. The single ink loading chamber 532 and the activator ink loading chamber 533 are respectively connected to the corresponding interfaces on the single printhead 591 through pipelines to meet the requirements of the single printhead 591.

[0169] The single ink filling pipeline device 540 includes a first ink filling component 543, a first connector 541 and a second connector 542. One end of the first connector 541 is connected to the single ink loading chamber 532 through a pipeline, and the other end has a first engaging portion. One end of the second connector 542 has a second engaging portion that matches the first engaging portion, and the other end is connected to the first ink filling component 543.

[0170] A one-way valve is installed in the first engagement part to seal the first connector 541 when the first connector 541 is disengaged from the second connector 542, preventing the single ink loading chamber 532 from communicating with the outside world and maintaining the internal pressure of the single ink loading chamber 532.

[0171] The activator ink filling pipeline device 550 includes a second ink filling assembly 553, a third connector 551 and a fourth connector 552. One end of the third connector 551 is connected to the activator ink loading chamber 533, and the other end has a third engaging portion. One end of the fourth connector 552 has a fourth engaging portion that matches the third engaging portion, and the other end is connected to the second ink filling assembly 553.

[0172] A one-way valve is installed in the third engagement part to seal the third connector 551 when the third connector 551 is disengaged from the fourth connector 552, preventing the activator ink loading chamber 533 from communicating with the outside world and maintaining the internal pressure of the activator ink loading chamber 533.

[0173] Furthermore, the first engaging part is inserted into the second engaging part, and the third engaging part is inserted into the fourth engaging part.

[0174] The engaging design of the first connector 541, the second connector 542, the third connector 551, and the fourth connector 552 makes the ink filling process more convenient and reliable. Users can fill ink through simple engaging operations, reducing operational complexity and the probability of errors.

[0175] One end of the first connector 541 is preferably connected to the monomer ink loading chamber 532 via a transparent flexible tube, and one end of the third connector 551 is preferably connected to the activator ink loading chamber 533 via a transparent flexible tube for easy observation.

[0176] In this embodiment, both the first ink filling component 543 and the second ink filling component 553 are preferably syringe components, which can perform convenient and fast ink filling operations while accurately controlling the amount of ink added.

[0177] In addition, both the first ink filling component 543 and the second ink filling component 553 can be automatic ink filling components to achieve automatic ink filling.

[0178] Furthermore, the inlets of both the monomer ink loading chamber 532 and the activator ink loading chamber 533 are preferably located at the center of the top of the chamber, ensuring that the added ink flows to the bottom of the chamber.

[0179] The individual ink filling line device 540 and the activator ink filling line device 550 are designed independently to ensure that different types of inks do not mix, further reducing the risk of corrosion.

[0180] The pressure source device 580 can provide negative pressure or positive pressure to match the single nozzle 591.

[0181] During the first working process, when ink is added to the single printhead 591 through the individual ink filling pipeline device 540 and the activator ink filling pipeline device 550 via the visible ink cartridge 530, the pressure source device 580 is activated to provide positive pressure. The purpose is to use the provided positive pressure (i.e., instantaneous ink pressing) to force the ink out of the corresponding hole of the single printhead 591, thereby cleaning the single printhead 591 and ensuring that the single printhead 591 is in optimal condition. Furthermore, the status of the single printhead 591 can be determined based on the printing test results.

[0182] Once the single printhead 591 is in the correct position, the pressure source device 580 provides the same negative pressure to the single ink loading chamber 532 and the activator ink loading chamber 533. At this time, the force of the negative pressure is balanced with the weight of the ink in the hole of the single printhead 591, ensuring the usability of the single printhead 591.

[0183] In this embodiment, the overflow ink cartridge 570 is configured as a safety feature. When the negative pressure provided by the pressure source device 580 causes the ink in the visible ink cartridge 530 to flow back, the overflow ink cartridge 570 is introduced into the overflow ink cartridge 570 to prevent the ink in the visible ink cartridge 530 from flowing back to the pressure source device 580 when the negative pressure is high, thereby protecting the pressure source device 580.

[0184] It should be noted that the pressure source device 580, the first ink filling component 543, and the second ink filling component 553 in the figure are all schematic and do not limit the scope of protection of this application.

[0185] In addition, the pressure source device 580 can completely empty the ink cartridge after printing by providing positive pressure. This is especially important for DNA ink, which has a short shelf life and needs to be replaced frequently, and can effectively prevent ink deterioration and contamination.

[0186] Specifically, the visual ink cartridge 530 includes a cartridge body 531 and a cover plate 535 disposed on the top of the cartridge body 531. The cover plate 535 has a first through hole and a second through hole that are matched with the single ink loading chamber 532 and the activator ink loading chamber 533. One end of the first connector 541 is connected to the first through hole through a pipe. One end of the third connector 551 is connected to the second through hole through a pipe.

[0187] The single ink loading chamber 532 and the activator ink loading chamber 533 are opened inside the box body 531, and the longitudinal axis of the single ink loading chamber 532 and the activator ink loading chamber 533 is arranged parallel to the longitudinal axis of the box body 531.

[0188] The top of the box 531 has a first annular groove 5341 and a second annular groove 5342 that match the single ink loading chamber 532 and the activator ink loading chamber 533, respectively. Annular elastic sealing rings are installed in both the first annular groove 5341 and the second annular groove 5342.

[0189] Furthermore, the annular elastic sealing ring is interference-fitted with the annular groove; the height of the annular elastic sealing ring is higher than the depth of the annular groove, ensuring that the annular elastic sealing ring is compressed after the cover plate 535 is installed, thus achieving a better sealing effect.

[0190] After the cover plate 535 is assembled with the box body 531, the annular elastic sealing ring is elastically compressed to be flush with the annular groove under the pressure of the cover plate 535. The annular elastic sealing ring is used to seal the box body 531 and effectively isolate the two chambers inside the box body 531 from the outside.

[0191] The side of the housing 531 has a first ink outlet 5343 and a second ink outlet 5344, which are respectively connected to the single ink loading chamber 532 and the activator ink loading chamber 533. The first ink outlet 5343 is connected to the first inlet of the single printhead 591 through a first adapter 5345 and a first pipe 561. A first filter 562 is provided on the first pipe 561. The second ink outlet 5344 is connected to the second inlet of the single printhead 591 through a second adapter 5346 and a second pipe 563. A second filter 564 is provided on the second pipe 563. The filter ensures high quality of ink addition to the single printhead 591.

[0192] In this embodiment, both the first filter 562 and the second filter 564 are preferably butterfly filters.

[0193] Furthermore, a first manual valve is provided between the first filter 562 and the first ink outlet 5343 for controlling the opening and closing of the first pipeline 561, that is, for controlling the opening and closing of the single ink loading chamber 532 and the single printhead 591; a second manual valve is provided between the second filter 564 and the second ink outlet 5344 for controlling the opening and closing of the second pipeline 563, that is, for controlling the opening and closing of the activator ink loading chamber 533 and the single printhead 591.

[0194] The volume ratio of the overflow ink cartridge 570 to the volume of the buffer gas cylinder in the pressure source device 580 is Δ, where 0.5≤Δ≤1.

[0195] In addition, the overflow ink cartridge 570 can act as a buffer when the pressure source device 580 switches between positive and negative pressure, thus avoiding large pressure fluctuations.

[0196] In this embodiment, the visible ink cartridge 530 can be a transparent or semi-transparent box, which makes it easy to clearly observe the remaining corresponding ink content inside the two chambers.

[0197] Furthermore, the Visual Ink Cartridge 530 is preferably made of a brand-new corrosion-resistant PP material (i.e., a non-metallic material). This material is not only corrosion-resistant, but also ensures the quality of the ink and the printing effect, effectively solving the corrosion problem of DNA ink in traditional aluminum alloy ink cartridges.

[0198] Referring to Figure 14, a first through hole 536 is provided between the single ink loading chamber 532 and the activator ink loading chamber 533; a second through hole 537 is provided on the side of the box body 531, and the pressure source device 580 is connected to the second through hole 537 through a pipeline.

[0199] The second through hole 537 is preferably located on the side of the activator ink loading chamber 533.

[0200] When ink is added to the single printhead 591 through the single ink filling pipeline device 540, the activator ink filling pipeline device 550, and the visual ink cartridge 530, the pressure source device 580 is activated to provide positive pressure. The pressure can be transmitted into the chamber through the second through hole 537, and the first through hole 536 ensures that the pressure acting on the two chambers is consistent.

[0201] In this embodiment, the distance between the first through hole 536 and the bottom of the cover plate 535 is H11, and the height of the box body 531 is H21, 0 < H11 / H21 < 0.1, ensuring that the first through hole 536 is close to the top, effectively preventing the ink in the single ink loading chamber 532 and the activator ink loading chamber 533 from mixing during the ink filling process.

[0202] The second through hole 537 is set at the same height as the first through hole 536.

[0203] In another embodiment, the monomer ink loading chamber 532 is isolated from the activator ink loading chamber 533; a first pressure hole is provided on the side of the monomer ink loading chamber 532, and a second pressure hole is provided on the side of the activator ink loading chamber 533; the pressure source device 580 is connected to the first pressure hole and the second pressure hole through a transmission pipeline assembly; the transmission pipeline assembly includes a main pipeline connected to the pressure source device 580 and a first sub-pipeline and a second sub-pipeline arranged in parallel, one end of the first sub-pipeline is connected to the main pipeline, and the other end is connected to the first pressure hole; one end of the second sub-pipeline is connected to the main pipeline, and the other end is connected to the second pressure hole.

[0204] When the pressure source device 580 is activated to provide positive or negative pressure, the pressure provided to the two chambers can be ensured to be consistent through the configuration of the transmission pipeline assembly.

[0205] The distance between the first pressure hole and the bottom of the cover plate 535 is H12, and the height of the box body 531 is H21, where 0 < H12 / H21 < 0.1; the distance between the second pressure hole and the bottom of the cover plate 535 is H13, and the height of the box body 531 is H21, where 0 < H13 / H21 < 0.1. This ensures that the first and second pressure holes are located close to the top to prevent the mixing of inks added to the two chambers.

[0206] In this embodiment, the size of the visual ink cartridge is matched with the size of the printhead device to ensure compact installation and effectiveness.

[0207] Furthermore, the distance between the bottom of the visible ink cartridge and the bottom of the printhead unit is h, 200mm≤h≤300mm, to ensure the ink filling effect of the printhead unit without affecting the normal operation of the printhead unit.

[0208] In this embodiment, the visible ink cartridge is preferably positioned directly above the printhead assembly; the first ink outlet 5343 is matched with the bottom of the single ink loading chamber 532, and the second ink outlet 5344 is matched with the bottom of the activator ink loading chamber 533, ensuring that the ink entering the single printhead 591 from the two chambers flows effectively and continuously.

[0209] The first ink outlet 5343 and the second ink outlet 5344 are matched with the first inlet and the second inlet of the single printhead 591. Furthermore, the first ink outlet 5343 and the second ink outlet 5344 are preferably located directly above the first inlet and the second inlet of the single printhead 591 to ensure that the ink flows quickly to the corresponding inlet of the single printhead 591 under the action of ink filling force and gravity.

[0210] The printing device disclosed in this application uses a visible ink cartridge, which has an independently configured individual ink loading chamber and an activator ink loading chamber. This allows for precise control of the capacity of each chamber, avoiding ink waste caused by excessive capacity in traditional auxiliary ink cartridges. The independent configuration of each chamber allows for optimized design to meet the small-volume requirements of DNA ink, ensuring the exact amount of ink used each time, thereby reducing unnecessary ink waste and storage space occupation. One-way valves are installed in the first and third locking sections to ensure that ink flows only in one direction during ink filling, preventing contamination caused by ink backflow. A pressure source device provides negative pressure matched to the printhead assembly, ensuring stable operation of the printhead assembly. The solution disclosed in this application has a simple structure, flexible ink filling, and long service life, effectively providing print quality and efficiency.

[0211] In existing technologies, the capacity of traditional auxiliary ink cartridges and automatic ink supply systems are unsuitable for the small-volume, short-term demand for DNA ink; the materials of traditional auxiliary ink cartridges cannot withstand the corrosiveness of DNA ink, failing to guarantee ink quality and printing results; traditional designs cannot meet the requirement of using different inks for multiple printing tasks simultaneously, leading to ink mixing and shortened shelf life. The printing device disclosed in this application proposes an ink supply system more suitable for DNA printing needs, including smaller capacity, a completely emptying function, and the selection of corrosion-resistant materials, thereby improving printing quality and efficiency. It not only solves the problems of ink waste, storage space occupation, pollution and deterioration, and ink corrosion in DNA printing applications of traditional inkjet printing technology, but also improves the ease of operation and reliability of the system. These improvements give this solution significant advantages in the field of DNA chip synthesis printing, and can better meet the special requirements of DNA printing for ink management and auxiliary ink cartridge materials.

[0212] It should be noted that the inks used in this application are monomer inks and activator inks. When other types of inks are needed, they can be flexibly replaced, and new products can be developed. That is, when adding ink to at least two types of inks, the chambers and ink adding devices in this application can be set up to correspond to the ink types. All of these are within the protection scope of this application, so they will not be described in detail here.

[0213] Referring to Figures 15 and 16, the printhead device includes multiple independently configured single printheads 591 and a multi-printhead position adjustment device for adjusting the positions of the multiple single printheads. The multiple single printheads 591 are correspondingly installed on the multi-printhead position adjustment device. The multi-printhead position adjustment device is used to adjust the positions of the multiple single printheads in inkjet printing, ensuring that the positional accuracy of the multiple single printheads meets the requirements and achieving accurate printing.

[0214] The multi-nozzle position adjustment device includes a nozzle support plate 592, on which a first groove 5921 is provided. The first groove 5921 has a plurality of nozzle mounting positions. In this embodiment, five nozzle mounting positions are provided for corresponding installation of five single nozzles 591.

[0215] Specifically, a number of through holes 5922 are provided in the first groove 5921. Each through hole 5922 is staggered from the inkjet area of ​​the single printhead 591, so that the inkjet printing of the single printhead 591 is not affected after the single printhead 591 is installed.

[0216] Each through hole 5922 has a positioning hole 593 on both sides that fits with the positioning pin on the single nozzle 591. This facilitates the initial positioning of the single nozzle 591 at each through hole 5922 without affecting the fine adjustment of the position of the single nozzle 591, so that the positioning pin will not disengage from the corresponding positioning hole 593.

[0217] In this embodiment, each through hole 5922 and its corresponding positioning hole 593 constitute a nozzle mounting position.

[0218] Furthermore, the longitudinal axis of the through hole 5922 is set parallel to the longitudinal axis of the single nozzle 591, that is, the through hole 5922 is a long slot hole, which provides movable space for the single nozzle 591 to move back and forth and swing left and right.

[0219] Each nozzle mounting position is equipped with a horizontal deflection adjustment component; specifically, the horizontal deflection adjustment component includes an elastic element 5971 with compression preload on the first side of the single nozzle 591 and an adjustment element 5972 on the second side of the single nozzle 591. The line connecting the center of the adjustment element 5972 and the center of the elastic element 5971 is set at a preset angle with the longitudinal axis of the single nozzle 591.

[0220] Among them, the elastic element 5971 is preferably a spring, and the adjusting element 5972 is preferably a tapered screw, which is simple and reliable.

[0221] The preset angle is α, where 60° < α < 80°. This means that the adjusting element 5972 and the elastic element 5971 are misaligned to ensure that the single nozzle 591 can be adjusted to swing left and right.

[0222] When the adjusting component 5972 is a conical screw, since the outer side of the conical screw is conical, that is, the outer diameter increases from the head to the tail, when the conical screw is moved downwards by screwing, the outer diameter in contact with the single nozzle 591 will increase, that is, it will exert a leftward thrust on the second side of the single nozzle 591, that is, realize the clockwise rotation adjustment (i.e., rightward yaw adjustment) of the single nozzle 591 in the horizontal plane. The elastic component 5971 set on the second side of the single nozzle 591 will be compressed. Under the combined action of the compressed elastic component 5971, the adjusted adjusting component 5972 on the second side of the single nozzle 591, and the corresponding positioning pin and positioning hole 593, the single nozzle 591 after position adjustment is fixed.

[0223] When the conical screw is moved upwards, the outer diameter in contact with the single nozzle 591 decreases. Under the compression and pre-tightening force of the elastic element 5971 on the first side of the single nozzle 591, a rightward thrust is applied to the first side of the single nozzle 591, thus achieving a pseudo-clockwise rotation adjustment (i.e., leftward sway adjustment) of the single nozzle 591 in the horizontal plane. The extended elastic element 5971 is still in a compressed state. Under the combined action of the elastic element 5971, the adjusted element 5972 on the second side of the single nozzle 591, and the corresponding positioning pin and positioning hole 593, the single nozzle 591 is fixed after the position adjustment.

[0224] The distance from the center of the elastic element 5971 to the transverse central axis of the single nozzle 591 is H1, the distance from the center of the adjusting element 5972 to the transverse central axis of the single nozzle 591 is H2, and the length of the single nozzle 591 is H3, where 0 < H1 < H2 < H3 / 2. This means that both the elastic element 5971 and the adjusting element 5972 are located on one side of the transverse central axis of the single nozzle 591, and on opposite sides of the longitudinal central axis of the single nozzle 591. The adjusting element 5972 is located closer to the end of the single nozzle 591, meaning the elastic element 5971 is closer to the transverse central axis of the single nozzle 591 than the adjusting element 5972. Because the distance from the center of the adjusting element 5972 to the transverse central axis of the single nozzle 591 is less than half the length of the single nozzle 591, the effective effect of the elastic element 5971 and the adjusting element 5972 on the yaw force of the single nozzle 591 can be effectively ensured.

[0225] In this embodiment, a horizontal movement adjustment assembly is installed on the nozzle support plate 592. The horizontal movement adjustment assembly includes a leveling screw and a spring plunger 5983 with a preset compression elastic force. That is, the spring plunger 5983 has a certain compression during assembly, and the amount of compression can be adjusted by adjusting the position of the plunger.

[0226] The nozzle support plate 592 has a first hole 5981 on one side to accommodate the leveling screw, and a second hole 5982 on the other side to accommodate the spring plunger 5983. The second hole 5982 is matched with the first hole 5981. The top of the leveling screw is pressed against the third side of the single nozzle 591, and the top of the spring plunger 5983 is pressed against the fourth side of the single nozzle 591 to ensure the fixation of the single nozzle 591.

[0227] Preferably, the longitudinal center axis of the first hole 5981 coincides with the longitudinal center axis of the second hole 5982; preferably, the longitudinal center axis of the first hole 5981 coincides with the longitudinal center axis of the through hole 5922.

[0228] Among them, the leveling screw has the degree of freedom to move along the first hole 5981, and the spring plunger 5983 has the degree of freedom to move along the second hole 5982. That is, the position of the single nozzle 591 can be adjusted by adjusting the screw depth in the first hole 5981.

[0229] When the leveling screw moves inward, the top of the leveling screw pushes the single nozzle 591 closer to the spring plunger 5983, while compressing the spring plunger 5983. When the leveling screw moves outward, the single nozzle 591 moves closer to the leveling screw under the push of the preset compression elastic force of the spring plunger 5983. At this time, the spring plunger 5983 still has a compression preload.

[0230] In this application, a set of horizontal movement adjustment components can be provided at each nozzle mounting position for precise adjustment of the position of each individual nozzle 591.

[0231] Furthermore, in this embodiment, only four sets of horizontal movement adjustment components can be set, meaning the number of horizontal movement adjustment components can be one less than the number of single printheads 591. For the single printheads 591 arranged side by side from left to right, the leftmost single printhead 591 does not need a horizontal movement adjustment component. Therefore, after the leftmost single printhead 591 is installed, it can be used as a reference, meaning the leftmost single printhead 591 can be adjusted left and right, and its front and back position is the reference position, without needing to be adjusted forward and backward. For the remaining four single printheads 591, the front and back position and left and right tilt position can be adjusted through the corresponding horizontal movement adjustment components and horizontal tilt adjustment components. By observing the actual ink droplet landing points, the positions of the single printheads 591 are adjusted, and finally, the five single printheads 591 are in a state of being parallel to each other and aligned front and back. This ensures that at the same reaction site, the ink droplets of the five single printheads 591 can all land within the reaction site.

[0232] Referring to Figures 16 and 17, the bottom of the nozzle support plate 592 includes a protrusion 5941 and a first edge portion 5942 and a second edge portion 5961 disposed opposite to each other. The protrusion 5941 is located between the first edge portion 5942 and the second edge portion 5961, and the area of ​​the protrusion 5941 is larger than the area of ​​the first groove 5921. The bottom surface of the protrusion 5941 and the upper surface of the nozzle mounting position are both precision machined surfaces with a high degree of flatness.

[0233] The distance from the first edge portion 5942 to the top of the nozzle support plate 592 is the same as the distance from the second edge portion 5961 to the top of the nozzle support plate 592; the distance from the first edge portion 5942 to the top of the nozzle support plate 592 is less than the distance from the protrusion 5941 to the top of the nozzle support plate 592, that is, from the side view, the bottom surface of the protrusion 5941 is lower than the first edge portion 5942 and the second edge portion 5961.

[0234] The first edge portion 5942 has a first adjustment through hole 5951 and a second adjustment through hole 5952, and the second edge portion 5961 has a third adjustment through hole 5962 and a fourth adjustment through hole 5963; the first adjustment through hole 5951 and the third adjustment through hole 5962 are symmetrically arranged with respect to the transverse central axis of the nozzle support plate 592; the first adjustment through hole 5951 and the second adjustment through hole 5952 are symmetrically arranged with respect to the longitudinal central axis of the nozzle support plate 592.

[0235] The multi-nozzle position adjustment device also includes an overall leveling assembly, which includes a first adjusting screw 5991, a second adjusting screw 5992, a third adjusting screw 5993, and a fourth adjusting screw 5994 respectively installed in the first adjusting through hole 5951, the second adjusting through hole 5952, the third adjusting through hole 5962, and the fourth adjusting through hole 5963; the first adjusting screw 5991, the second adjusting screw 5992, the third adjusting screw 5993, and the fourth adjusting screw 5994 all have degrees of freedom of movement.

[0236] Specifically, when the first adjusting screw 5991 and the second adjusting screw 5992 on the left are rotated or pushed downwards, the left side of the overall nozzle support plate 592 will be pushed upwards; when the third adjusting screw 5993 and the fourth adjusting screw 5994 on the right are rotated or pushed downwards, the right side of the overall nozzle support plate 592 will be pushed upwards, thereby adjusting the overall flatness. By repeatedly fine-tuning the four adjusting screws, the overall flatness of the nozzle support plate 592 can be made to meet the design requirements, ensuring the flatness of the upper surface of the nozzle mounting position.

[0237] The multi-nozzle position adjustment device disclosed in this application can precisely control the position and direction of multiple individual nozzles to meet complex application requirements. By setting grooves and nozzle mounting positions on the nozzle carrier plate and equipping the nozzle mounting positions with horizontal deflection adjustment components, the device enables minute angle adjustments of individual nozzles. Specifically, the horizontal deflection adjustment components, through the cooperation of elastic elements and adjusting elements, can achieve deflection of individual nozzles within a specific angle range, allowing each individual nozzle to independently adjust its minute left and right sway angles, thereby adapting to different working scenarios or material handling requirements. Through the leveling screw and spring plunger in the horizontal movement adjustment components, pressure can be applied to both sides of the individual nozzles respectively. By adjusting the position of these components, precise movement and positioning of the individual nozzles on the horizontal plane can be achieved. This device not only improves the flexibility and accuracy of individual nozzle use but also facilitates the maintenance and replacement of individual nozzles.

[0238] This application discloses a multi-nozzle position adjustment device that addresses the shortcomings of existing technologies, particularly the challenges of precise calibration and adjustment of single-nozzle positions in high-throughput synthesis chips, and proposes an innovative solution. Specifically, the device includes a nozzle carrier plate, a horizontal deflection adjustment component, and a horizontal movement adjustment component. These components work together to ensure high-precision position adjustment of a single nozzle under complex operating environments, thereby improving printing accuracy and efficiency. The combination of the horizontal deflection adjustment component and the horizontal movement adjustment component enables precise adjustment of the single nozzle position. The horizontal deflection adjustment component utilizes the synergistic effect of elastic and adjusting elements to ensure free horizontal deflection of the single nozzle, adapting to different printing requirements. The horizontal movement adjustment component, through the setting of a leveling set screw and a spring plunger, further improves the vertical position adjustment accuracy of the single nozzle, ensuring that the reaction reagent ejected from the nozzle falls precisely within the reaction site.

[0239] Traditional single-nozzle position adjustment techniques typically require large adjustment structures around the single nozzle, occupying considerable space. The device in this application, through optimized design, reduces the space occupied by the adjustment mechanism, allowing more single nozzles to be installed within a limited space, thereby increasing the overall throughput of the system. The device is designed for ease of operation; users can achieve precise adjustment of each nozzle through simple mechanical operations, eliminating the need for complex calibration processes and significantly reducing operational difficulty. Precise position adjustment prevents reaction reagents from being sprayed into non-target areas, thus reducing the possibility of reaction failure and contamination, and improving the success rate of DNA synthesis reactions. This device is particularly suitable for high-throughput synthesis chips, capable of handling complex situations with a large number of reaction sites, small spacing, and varying site sizes, ensuring high precision even with large printing coverage areas.

[0240] Referring to Figures 18 and 19, the moisturizing device includes a drive assembly 610, a transmission assembly, and an ink absorption assembly 650. A first slider 621 is mounted on the power output end of the drive assembly 610. The first slider 621 has a degree of freedom to move along the longitudinal axis of the power output end of the drive assembly 610, that is, the first slider 621 can move under the drive of the drive assembly 610.

[0241] Specifically, the drive assembly 610 includes a drive motor 611 and a lead screw 612 mounted on the power output end of the drive motor 611. The drive motor 611 is mounted on a horizontal support plate 661, and a linear guide rail 662 is provided on the horizontal support plate 661 to engage with the bottom of the first slider 621. The linear guide rail 662 is arranged parallel to the lead screw 612. The first slider 621 is sleeved on the lead screw 612 and threadedly connected to the lead screw 612. The linear guide rail 662 ensures the linear movement of the first slider 621.

[0242] In this embodiment, the drive motor 611 is biased, which ensures that the ink-absorbing top plate 641 is located in a relatively central position, while also ensuring that the overall structure is compact and occupies less space.

[0243] Referring to Figures 19 and 20, a second slider 622 is fixedly provided on the side of the first slider 621. In this embodiment, the first slider 621 is an L-shaped slider. The bottom of the first side of the L-shaped slider is provided with a locking groove that matches the linear guide rail 662. The second slider 622 is fixedly provided on the second side of the L-shaped slider.

[0244] The transmission assembly 630 includes a guide rail 631 that engages with the second slider 622 and a vertical plate 632 for mounting the guide rail 631. The longitudinal axis of the guide rail 631 is set at a preset angle with the moving direction of the first slider 621. The guide rail 631 has the freedom to rise or fall under the drive of the second slider 622.

[0245] The preset included angle is α, where 30°≤α≤45°.

[0246] A moisturizing ink pad assembly 640 is fixedly provided on the top of the upright plate 632; the moisturizing ink pad assembly 640 includes an ink-absorbing top plate 641, on which a second groove 642 (i.e., a chamber) is provided, and a moisturizing pad 643 matching the printhead is provided in the second groove 642. In this embodiment, matching the printhead can be understood as the moisturizing pad 643 being sufficient to fit tightly against the corresponding printhead, satisfying the moisturizing of the area corresponding to the printhead; wherein, each printhead corresponds to one moisturizing pad 643, each printhead can be maintained individually, and the ink and gas generated during maintenance are absorbed by the corresponding moisturizing pad 643.

[0247] The ink suction assembly 650 includes an ink suction pump and a waste ink storage bottle (not shown in the figure) connected to the ink suction pump. The ink suction pump is connected to the second groove 642 through a pipeline. Under the action of the ink suction pump, the second groove 642 forms a negative pressure environment facing the corresponding printhead.

[0248] When the printing device is in standby mode, it is located directly above the groove. Under the action of the ink pump, the groove forms a negative pressure environment facing the corresponding printhead. That is, when the ink pump is started, it provides negative pressure and forms a negative pressure environment in the area of ​​the second groove 642.

[0249] Specifically, before flash printing, ink pressing, and cleaning, the ink suction pump is activated to create a negative pressure environment within the second groove 642 (i.e., the moisturizing pad 643 and its surrounding area). When there are reagents that are about to evaporate or diffuse at the printhead, the negative pressure of the negative pressure environment will instantly suck away these reagents (i.e., the chemical reagents generated during printhead maintenance) and transfer them to the waste ink storage bottle through the ink suction pump. This ensures that there are almost no evaporating or diffused reagents in the surrounding environment, effectively reducing or even avoiding printhead clogging and uneven ink spraying caused by ink residue, dust, ink diffusion and fusion during use, thus ensuring printing quality and normal operation of the equipment.

[0250] Furthermore, before flash printing or waste ink pressing is performed, the moisturizing pad 643 will rise to the designated position in advance, and the ink pump will start a certain time in advance to form a negative pressure environment, reducing the diffusion of ink into the environment caused by ink pressing or flash printing.

[0251] After a flash or waste ink press is performed, the moisturizing pad 643 will descend with a delay, and the ink pump will shut down for a period of time to maintain a negative pressure environment and prevent ink residue caused by the waste ink press or flash from spreading into the environment.

[0252] In operation, the drive motor 611 starts, driving the lead screw 612 to rotate, which in turn drives the first slider 621 to move along the lead screw 612. When the first slider 621 moves closer to the drive motor 611, it is equivalent to the second slider 622 moving closer to the drive motor 611. Since the second slider 622 is fixed on the first slider 621, the second slider 622 can only move horizontally. Through the engagement and matching of the second slider 622 with the guide rail 631, the guide rail 631 will be driven to move upward. Since the guide rail 631, the upright plate 632, and the moisturizing ink pad assembly 640 are all fixedly connected, the moisturizing ink pad assembly 640 will be driven to move upward. Conversely, when the first slider 621 moves away from the drive motor 611, the transmission component will drive the moisturizing ink pad assembly 640 to move downward.

[0253] In this embodiment, the upright plate 632 includes a first plate for mounting guide rails 631 and a vertical connecting plate fixedly connected to the first plate. The top of the vertical connecting plate is fixedly connected to the ink-absorbing top plate 641.

[0254] Furthermore, an elastic element is provided between the moisturizing pad 643 and the second groove 642, preferably a spring; when the elastic element is in its naturally extended state, the moisturizing pad 643 is higher than the second groove 642, and when it is pressed on the nozzle surface, the spring can provide a certain buffering effect, thereby protecting the nozzle.

[0255] Preferably, a through hole is provided at the bottom of the second groove 642. The ink pump is connected to the through hole through a pipeline. When the moisturizing pad 643 comes into contact with the printhead, the moisturizing pad 643 is preferably lower than the top of the second groove 642 to ensure that the reagent from the printhead is absorbed by the moisturizing pad 643 and will not escape into the surrounding environment. The negative pressure environment formed in this way will surround the entire moisturizing pad 643. Therefore, the reagent present in the second groove 642 and the moisturizing pad 643 can be quickly collected into the waste ink storage bottle under the action of suction.

[0256] Furthermore, the center of the moisturizing pad 643 is preferably aligned with the center of the corresponding nozzle; the top area of ​​the moisturizing pad 643 is smaller than the surface cross-section of the nozzle, but larger than the sum of the areas of all the holes on the nozzle, ensuring effective maintenance of the nozzle while avoiding any waste in the design of the moisturizing pad 643.

[0257] In this embodiment, the distance from the top of the moisturizing pad 643 to the lower surface of the nozzle in the initial position is H1, and the rising distance of the moisturizing pad 643 from the initial position to the target position is H2, Δ=H2-H1, 1mm≤Δ≤3mm. By setting it in this way, it can be ensured that the moisturizing pad 643 is in close contact with the nozzle, thus ensuring the maintenance effect on the nozzle.

[0258] In this embodiment, a first side plate 663, a second side plate 664, a third side plate 665, a fourth side plate 666, and a first bearing mounting plate 667 are provided on the horizontal support plate 661. The first side plate 663, the second side plate 664, the third side plate 665, and the fourth side plate 666 are all fixedly installed on the bottom of the first bearing mounting plate 667. The first side plate 663 is arranged adjacent to the second side plate 664 and opposite to the third side plate 665. The drive motor 611 is installed on the horizontal support plate 661 through the second side plate 664. The ink absorption assembly 650 is installed on the outside of the fourth side plate 666.

[0259] Furthermore, an L-shaped first sheet metal cover 671 is provided on the outer side of the first side plate 663 and the second side plate 664, and an L-shaped second sheet metal cover 672 is provided on the outer side of the second side plate 664 and the third side plate 665. The first sheet metal cover 671, the second sheet metal cover 672 and the fourth side plate 666 form a protective cover around the perimeter.

[0260] The outer side of the ink-absorbing top plate 641 is provided with a sheet metal upper cover 646 to protect the ink-absorbing top plate 641.

[0261] The bottom of the ink-absorbing top plate 641 is provided with several columns 644, and each column 644 is fitted with a shaft support seat 645 that is fixedly installed on the bottom of the ink-absorbing top plate 641; the top of the first bearing mounting plate 667 is equipped with a linear bearing component 6671 that matches the column 644, and the linear bearing component 6671 is matched with the shaft support seat 645. The setting of the shaft support seat 645 can ensure that the ink-absorbing top plate 641 will not directly contact the linear bearing component 6671, thereby protecting the ink-absorbing top plate 641 during the lifting process.

[0262] The lower end of the column 644 extends through the shaft support 645 and then extends into the linear bearing 6671. When the ink-absorbing top plate 641 rises or falls, the column 644 can rise or fall relative to the linear bearing 6671. That is, the column 644 has the freedom of movement to rise or fall along the longitudinal axis of the linear bearing 6671. The linear bearing 6671 can guide the column 644.

[0263] Furthermore, a tension spring is provided between the top of the first bearing mounting plate 667 and the ink-absorbing top plate 641. The tension spring has a preset tension force, which pulls the ink-absorbing top plate 641 close to the first bearing mounting plate 667 when the ink-absorbing top plate 641 is in its initial assembly state (i.e., a downward tension force). During the upward movement of the ink-absorbing top plate 641, it still has a downward tension force, ensuring the stability of the ink-absorbing top plate 641 and preventing unnecessary movement or vibration when no external force is applied. At the same time, when the ink-absorbing top plate 641 is subjected to external force to rise or fall, the tension spring can provide a certain buffering effect, absorbing part of the impact force, thereby protecting the relevant components from damage.

[0264] Referring to Figure 21, in this embodiment, a limit sensor 633 is installed on the upright plate 632; a first bracket and a second bracket are installed on the side of the first side plate 663, a first sensor is installed on the first bracket, and a second sensor is installed on the second bracket; the first sensor and the second sensor are both configured to correspond to the limit sensor 633.

[0265] The first bracket is positioned above the second bracket, meaning the first sensor is positioned above the second sensor. When the second sensor detects the limit sensor 633, it indicates that the upright plate 632 has risen to the preset humidity position, thus triggering the drive motor 611 to stop.

[0266] When the second sensor detects the limit sensor 633, it indicates that the upright plate 632 has descended to the preset origin position, and then the drive motor 611 is triggered to stop.

[0267] In this embodiment, a slider limiting member 668 is installed at one end of the linear guide 662 to limit the first slider 621 and prevent the first slider 621 from slipping off the lead screw 612; a motor protection block 669 is installed at the other end of the linear guide 662 to prevent the first slider 621 from damaging the drive motor 611.

[0268] In this embodiment, the engagement of the second slider 622 with the guide rail 631 ensures relative movement between them. Specifically, the fixedly connected transmission assembly and the moisturizing ink pad assembly 640 can be considered as a whole. The horizontal movement of the whole is limited by the linear bearing 6671, which is matched with the column 644 and installed on the top of the first bearing mounting plate 667, so that the whole can only rise or fall. Therefore, when the second slider 622 and the first slider 621 move horizontally along the lead screw 612, they act on the guide rail 631, which controls the rise or fall of the guide rail 631, which is equivalent to controlling the rise or fall of the moisturizing ink pad assembly 640.

[0269] In this implementation, the number of ink pumps and waste ink storage bottles connected to the ink pumps are set to correspond to the number of moisturizing pads 643 and the number of printheads, that is, one printhead corresponds to one moisturizing pad 643, one ink pump, and one waste ink storage bottle.

[0270] When there are multiple printheads, the multiple second grooves 642 are arranged in parallel, and the corresponding multiple ink pumps are arranged in parallel, which makes the installation simple and efficient, while reducing the path of the connecting pipeline.

[0271] Each ink pump has an inlet and an outlet, with the inlet connected to a through-hole via piping. When there are multiple ink pumps, each outlet is connected to a multi-way connector via a sub-pipeline. The multi-way connector is then connected to a waste ink storage bottle via a main pipe. Because in this field, the entire humidification system, except for the waste ink storage bottle, is sealed in a glove box, the sub-pipelines, multi-way connectors, and main pipe effectively reduce the number of external connecting pipes and ensure a tight seal.

[0272] The moisturizing device disclosed in this embodiment is an innovative device designed to address the shortcomings in the precision and effectiveness of printhead maintenance in existing technologies, particularly the challenges faced by printheads interacting with chemical reagents in the field of DNA synthesis. It fully considers the unique characteristics of the inks used in DNA synthesis and the stringent environmental requirements of the synthesis process. Specifically, through the synergistic action of the drive and transmission components, the printhead can be precisely mechanically adjusted. The design of the first slider, second slider, and guide rail allows the horizontal movement of the first slider to be converted into the upward or downward movement of the guide rail, thereby precisely controlling the distance between the moisturizing ink pad assembly and the printhead, achieving precise lifting and lowering control, and improving the accuracy of printhead maintenance. The design of the ink-absorbing assembly, especially the combination of the ink pump and the groove, creates a negative pressure environment facing the printhead. This negative pressure effectively prevents improper ink diffusion, reduces the volatilization of chemical reagents and the diffusion of useless components, ensuring environmental consistency and synthetic quality. The ink pump and waste ink storage bottle allow for the automatic discharge and storage of waste ink during maintenance, greatly simplifying the cleaning process, reducing the need for manual operation, and lowering the risk of human error.

[0273] In this application, the combined use of drive and transmission components, along with the integrated design of the negative pressure and humidification systems, effectively reduces reliance on specialized technicians, decreases maintenance complexity and costs, and improves maintenance efficiency. The humidification device proposed in this application effectively ensures control precision, reduces environmental impact, enhances humidification function, and improves ease of operation, providing a better technical solution for highly sensitive applications such as DNA synthesis, thereby significantly improving the accuracy and efficiency of DNA synthesis.

[0274] The device disclosed in this application, through the adoption of advanced cleaning technology and intelligent maintenance processes, can achieve efficient cleaning of the printhead, reduce ink residue and diffusion, thereby ensuring the accuracy and stability of DNA synthesis. At the same time, the operation process is simple, highly automated, and has low maintenance costs, meeting the practical needs of a wide range of DNA synthesis researchers and users.

[0275] In this application, based on the standby state of the DNA synthesis device, the drive component can be controlled to move the moisturizing ink pad assembly toward the printhead in the DNA synthesis device until the moisturizing pad in the moisturizing ink pad assembly covers the printhead (i.e., when it rises to a preset position) and then the movement stops. This position is the moisturizing position. The moisturizing pad in the moisturizing ink pad assembly covers the printhead, thus maintaining the state of the printhead, because under normal conditions, the printhead is always in a moisturizing state.

[0276] When a task instruction is received (including printing instructions, ink wiping instructions, and situations where the printhead is used or processed), the control drive component moves the moisturizing ink pad assembly away from the printhead in the DNA synthesis device until it returns to its original position (i.e., the initial position). After receiving the instruction from the moisturizing device that the moisturizing ink pad assembly returns to its original position, the instruction to start the DNA printing and synthesis device is issued, and the DNA synthesis device starts. Specifically, the X-axis printing module in the DNA synthesis device can then move freely.

[0277] When flash spraying / pressing ink is required (with the printhead stationary), the drive component is controlled to move the moisturizing ink pad component to the moisturizing / pressing position according to the flash spraying / pressing ink command. The moisturizing / pressing ink position is the position between the moisturizing position and the origin position, that is, the moisturizing / pressing ink position is above the origin position and below the moisturizing position.

[0278] Specifically, the distance from the moisturizing / pressing ink position to the printhead is greater than 0 and not greater than 2mm.

[0279] In this embodiment, an intelligent control strategy can ensure precise coordination of printhead status maintenance and task execution at different working stages. Specifically, by intelligently controlling the moisturizing ink pad assembly, it can automatically move to the moisturizing position, ensuring that the printhead is always moist under normal conditions, preventing the printhead from clogging or degrading due to dryness, especially in long-term standby or intermittent use. When a printing command or other task command is received, the moisturizing ink pad assembly automatically returns to its original position, freeing up space for normal printhead use, reducing the need for manual operation, and improving the accuracy and consistency of operation.

[0280] Based on task instructions (such as print instructions, wipe instructions, etc.), the drive components are precisely controlled to ensure that the moisturizing ink pad assembly moves to the correct position at the appropriate time. This precise coordination not only ensures effective moisturization of the printhead but also optimizes the workflow of the printing synthesis equipment and improves overall efficiency. The DNA synthesis equipment will only start after the moisturizing ink pad assembly has completely returned to its original position. This safe start-up mechanism ensures that printing will only begin when the printhead is ready, avoiding work interruptions or quality problems caused by poor printhead condition.

[0281] During flash spray / press ink operation, the moisturizing ink pad assembly moves to the moisturizing / press ink position, which is between the origin position and the moisturizing position. This ensures that the printhead can perform necessary processing without moving. This design completes the flash spray or press ink operation in place, avoiding unnecessary wear and errors of the printhead during movement.

[0282] The integrated moisturizing device works seamlessly with the DNA synthesis equipment, enabling precise control of flash / press ink operations and ensuring refined and efficient operation. Intelligent control of the maintenance device allows for precise positioning of the moisturizing ink pad assembly near or away from the printhead at the correct time, ensuring the most effective use of chemical reagents and minimizing waste. Simultaneously, it ensures consistency between the printhead and the surrounding environment, reducing improper diffusion and volatilization of chemical reagents and improving the stability and quality of the synthesis process.

[0283] This embodiment can achieve the goals of automated nozzle humidification management, coordinated optimization of task execution, flexibility of on-site processing operations, and reduction of chemical reagent waste and diffusion through intelligent control. These benefits together improve the efficiency and accuracy of DNA chip printing synthesis, ensuring the continuity of the synthesis process and high-quality output.

[0284] Referring to Figure 22, the third-party directional drive device 700 includes a support device 820 and a pressure plate lifting device 830 mounted on the support device. The support device 820 includes a support member and a support device mounted on top of the support member. The support device and the support member are connected by a horizontal adjustment assembly, and the support device has a horizontal adjustment degree of freedom. The pressure plate lifting device 830 includes a power component and a pressure plate assembly mounted on the power output end of the power component. The pressure plate assembly has a degree of freedom to move up and down along the longitudinal axis of the power output end of the power component.

[0285] The microfluidic device 800 includes a fluid system and a sealing channel device 840 installed below the pressure plate assembly. The pressure plate lifting device 830 and the sealing channel device 840 are both mounted on the support device 820. The pressure plate lifting device 830 is used to control the sealing between the sealing channel device 840 and the chip 811 to be pressed and placed in a preset position. After the sealing channel device 840 and the chip 811 to be pressed and sealed are sealed, a plurality of sealed reaction chambers 812 are formed.

[0286] The liquid circuit system includes an independently configured liquid supply unit, gas supply unit, and waste liquid collection unit. The liquid supply unit is connected to several sealed reaction chambers 812 via a liquid supply pipeline assembly to supply reagents to each sealed reaction chamber 812. The gas supply unit is connected to several sealed reaction chambers 812 via a gas supply pipeline assembly and can purge the corresponding chambers with supplied gas. The waste liquid collection unit is connected to several sealed reaction chambers 812 via a liquid extraction pipeline assembly to collect the liquid that has finished reacting in the corresponding chamber. The liquid supply unit and the gas supply unit are switched via a gas-liquid circuit switching unit.

[0287] In this embodiment, by partitioning and sealing and controlling fluid flow in partitions, the large-area reaction area is divided into several independent small reaction areas (i.e., several sealed reaction chambers 812). Matching the chip 811 forms an openable and closable reaction chamber, which can control different reagents to complete the required reaction process in the corresponding sealed reaction chamber 812. At the same time, it can control the formation of a smaller liquid film thickness. After the reaction is completed, the corresponding sealed reaction chamber 812 can be quickly cleaned, which effectively solves the problems of uneven liquid injection, incomplete cleaning, and incomplete purging in the large-area reaction area in the prior art. This makes it easier to realize the high-throughput large-size chip 811 synthesis reaction.

[0288] Referring to Figure 23, the support device 820 includes a support member and a support device mounted on top of the support member. The support device and the support member are connected by a horizontal adjustment assembly, and the support device has a degree of freedom for horizontal adjustment.

[0289] In this embodiment, the support includes two brackets 821 and a horizontal plate 822. The horizontal plate 822 and the two brackets 821 form a gantry structure.

[0290] The bracket 821 includes an L-shaped gantry base plate 8211 and a gantry vertical plate 8212. The gantry base plate 8211 and the gantry vertical plate 8212 are connected and fixed by screws to ensure the support stability of the bracket 821. The two brackets 821 are arranged back to back, that is, both L-shaped gantry base plates 8211 are arranged outward.

[0291] The horizontal plate 822 has three leveling component mounting parts arranged in an acute-angled triangle; the horizontal adjustment assembly includes three sets of leveling components respectively installed on the three leveling component mounting parts.

[0292] Each leveling assembly includes a spherical bearing 823, an adjusting screw, and a second bearing mounting plate 824. The second bearing mounting plate 824 is fixed to the gantry vertical plate 8212 by screws, and threaded holes are provided on the second bearing mounting plate 824.

[0293] One end of the adjusting screw is fixedly connected to the spherical bearing 823 by a locking cover, and the end protrudes from the spherical bearing 823 and is threadedly connected to the horizontal plate 822 and locked by a nut. The other end of the adjusting screw is threadedly connected to the threaded hole and can rotate, that is, the adjusting screw has the freedom to move along the longitudinal central axis of the threaded hole. Specifically, the horizontal plate 822 can be adjusted horizontally by adjusting the rotatable end of the adjusting screw with a hexagonal wrench.

[0294] In this embodiment, when the left side of the horizontal plate 822 is lower than the horizontal plane, the position of the two joint bearings 823 on the left side is adjusted to increase the distance between the joint bearings 823 and the corresponding second bearing mounting plate 824. Since the second bearing mounting plate 824 remains stationary, this is equivalent to raising the left side of the horizontal plate 822, thus ensuring that the horizontal plate 822 as a whole meets the preset level. Similarly, when the right side of the horizontal plate 822 is lower than the horizontal plane, the position of one joint bearing 823 on the right side is adjusted to increase the distance between the joint bearing 823 and the corresponding second bearing mounting plate 824. Since the second bearing mounting plate 824 remains stationary, this is equivalent to raising the right side of the horizontal plate 822, thus ensuring that the horizontal plate 822 as a whole meets the preset level. Of course, the horizontal adjustment of the corresponding position of the horizontal plate 822 can also be achieved by adjusting the two joint bearings 823 on the left side individually.

[0295] After the horizontal plate 822 is horizontally adjusted, the rotatable end of the joint bearing 823 is locked by a nut, and the horizontal plate 822 can be connected and fixed to the gantry vertical plate 8212 by screws and set screws.

[0296] Furthermore, a decorative panel 825 is also provided on the horizontal panel 822 for decorative purposes.

[0297] Referring to Figures 23 and 24, the pressure plate lifting device 830 is installed on the bearing device. The pressure plate lifting device 830 includes a power component and a pressure plate assembly installed at the power output end of the power component. The pressure plate assembly has the freedom to lift and lower along the longitudinal axis of the power output end of the power component.

[0298] The horizontal plate 822 has motor mounting holes 8221 and column mounting holes 8222. The power assembly includes a servo motor 831 and a reducer 832 mounted on the power output end of the servo motor 831. The reducer 832 is fixed in the motor mounting hole 8221. The power output end of the reducer 832 is provided with a T-shaped nut 833 and a T-shaped screw 834. The T-shaped nut 833 is fixedly connected to the power output end of the reducer 832. The T-shaped screw 834 is rotatably engaged with the T-shaped nut 833. That is, when the servo motor 831 is started, the reducer 832 drives the T-shaped nut 833 to rotate, which in turn drives the T-shaped screw 834 to rise or fall.

[0299] Among them, the reducer 832 is a high-precision spiral bevel gear reducer.

[0300] The pressure plate assembly includes a bearing assembly 835 installed at the free end of the T-shaped lead screw 834, several equalizing plates 836 disposed below the bearing assembly 835, and several guide members. Under the drive of the power assembly, the several equalizing plates 836, several guide members, and bearing assembly 835 rise or fall synchronously with the T-shaped lead screw 834.

[0301] Several guide members have one end mounted on at least two pressure equalizing plates 836, and the other end passes through the column mounting hole 8222, providing guidance for the lifting and lowering of the pressure equalizing plates 836, the guide members, and the bearing assembly 835. The longitudinal central axis of the guide members is parallel to the longitudinal central axis of the T-shaped lead screw 834.

[0302] In this embodiment, the bearing assembly 835 includes a bearing member and a bearing housing that supports the bearing member. Specifically, the bearing member is an angular contact bearing, which is installed in the bearing housing and pressed by a locking cap 838.

[0303] Preferably, three equalizing plates 836 are provided. The three equalizing plates 836 are fixed at equal intervals in parallel at the bottom of the bearing seat. The middle equalizing plate 836 is located directly below the T-shaped lead screw 834, and the two equalizing plates 836 on both sides are symmetrically arranged relative to the middle equalizing plate 836.

[0304] The guide component is preferably a ball spline 837, of which four ball splines 837 are provided; two ball splines 837 are installed on each of the two pressure equalizing plates 836 on both sides, and the spline sleeve of the ball spline 837 is fixedly connected to the horizontal plate 822. The spline sleeve of the ball spline 837 has the freedom to move along the axis of the spline shaft while maintaining circumferential positioning. In this embodiment, the spline sleeve is fixedly connected to the corresponding column mounting hole 8222. Therefore, when the spline sleeve is fixed, the spline shaft can move up and down relative to the spline sleeve.

[0305] In addition, several equalizing plates 836, several guide components, and bearing assemblies 835 can be manually raised and lowered. That is, the servo motor 831 is stationary, and the T-screw 834 is driven to rotate and raise and lower through an Allen wrench. This method is used for manual testing and troubleshooting.

[0306] In this embodiment, the switching between manual lifting control and automatic lifting control via servo motor 831 is achieved by locking cover 838. Locking cover 838 is fixed to bearing seat with screws to press the angular contact bearing. At the same time, locking cover 838 can lock or release T-screw 834. When locked, it is in electric mode, and when released, it is in manual mode.

[0307] Furthermore, a first position detector can be provided at the top of the horizontal plate 822, and a second position detector can be provided at the bottom of the horizontal plate 822, for detecting the lifting position of the ball spline 837, that is, the lifting position of the pressure equalizing plate 836.

[0308] Referring to Figures 25 to 27, the sealing flow channel device 840 is installed below the pressure plate assembly. The sealing flow channel device 840 includes a partitioned flow channel body 841, a sealing ring 850, and a sealing pressure plate 860. The partitioned flow channel body 841 has a stepped protrusion 842 on the side away from the pressure plate assembly. The sealing ring 850 is matched with the stepped protrusion 842. The sealing pressure plate 860 is installed around the sealing ring 850 and is fixedly connected to the partitioned flow channel body 841. The sealing ring 850 has several sub-rings 851, which protrude from the sealing pressure plate 860. The protruding portions of the several sub-rings 851 and the pressed solid phase chip 811 form several sealed reaction chambers 812.

[0309] Each sealed reaction chamber 812 has an inlet 870 and an outlet 880, both of which penetrate the partitioned flow channel body 841 and the stepped protrusion 842. Specifically, the inlet 870 and outlet 880 are located near their two edges to facilitate a complete reaction. In this embodiment, the inlet 870 is a straight hole perpendicular to the plane of the partitioned flow channel body 841, with one end on the upper plane of the partitioned flow channel body 841 and the other end penetrating the stepped protrusion 842 and communicating with the corresponding sealed reaction chamber 812. The outlet 880 is an L-shaped hole, with one end on the side of the partitioned flow channel body 841 and the other end penetrating the stepped protrusion 842 and communicating with the corresponding sealed reaction chamber 812, used to output the reacted reagent.

[0310] Referring to Figure 28, the stepped protrusion 842 includes a first layer protrusion 843 and a plurality of second layer protrusions 844 disposed outside the first layer protrusion 843, the plurality of second layer protrusions 844 being disposed adjacent to each other in sequence; the edges of the second layer protrusions 844 and the edges of the first layer protrusions 843 form a stepped structure, and the total area of ​​the plurality of second layer protrusions 844 is smaller than the area of ​​the first layer protrusions 843.

[0311] The peripheral edge of the sealing ring 850 is matched with the peripheral edge formed by the second layer protrusion 844 and the first layer protrusion 843, and the second layer protrusion 844 is within the sub-rings 851.

[0312] There is a preset gap between two adjacent second-layer protrusions 844; the common edge of two adjacent sub-rings 851 fits into the preset gap, ensuring the installation stability of the middle area of ​​the sealing ring 850 and the independent non-connection of adjacent sub-rings 851.

[0313] The sealing plate 860 has a groove that matches the peripheral edge of the sealing ring 850 to ensure that the sealing ring 850 is pressed and fixed around its periphery.

[0314] Referring to Figure 29, the cross-section of the sealing ring 850 is T-shaped. Specifically, each sub-ring 851 is described in detail. The sub-ring 851 includes a first sealing part 852 and a second sealing part 853. The cross-sections of the first sealing part 852 and the second sealing part 853 form a T-shaped structure, that is, the outer lateral dimension of the first sealing part 852 is larger than the outer lateral dimension of the second sealing part 853, and the inner lateral dimension of the first sealing part 852 is smaller than the inner lateral dimension of the second sealing part 853.

[0315] The second layer protrusion 844 includes a first contact portion 845 and a second contact portion 846. The first contact portion 845 is located between the second contact portion 846 and the first layer protrusion 843, and the outer lateral dimension of the first contact portion 845 is smaller than the outer lateral dimension of the second contact portion 846.

[0316] Specifically, the first sealing part 852 and the first contact part 845 are fitted together and pressed tightly. Specifically, the first sealing part 852 can be interference-fitted with the first contact part 845 to achieve close contact with the area of ​​the second layer protrusion 844 near the first layer protrusion 843.

[0317] The second sealing part 853 and the second contact part 846 are tightly abutted together, and the two are also interference fit to achieve a seal between them and the second layer protrusion 844.

[0318] The sealing pressure plate 860 includes a first pressure plate 861, a second pressure plate 862, a third pressure plate 863, and a fourth pressure plate 864 arranged in sequence with their ends pressed together. The first pressure plate 861, the second pressure plate 862, the third pressure plate 863, and the fourth pressure plate 864 form an annular structure that presses the sealing ring 850. The inner side of the annular structure is correspondingly arranged with the first contact portion 845 and the second contact portion 846, and the second contact portion 846 extends through the annular structure.

[0319] In the assembled state, the T-shaped inner protrusion of the sealing ring 850 is fitted into the first contact portion 845 (i.e., the area near the first layer protrusion 843) in the second layer protrusion 844, and the T-shaped straight portion of the sealing ring 850 is pressed against the second contact portion 846 (i.e., the area away from the first layer protrusion 843) in the second layer protrusion 844. Then, the sealing plate 860 acts on the T-shaped outer protrusion of the sealing ring 850, and the sealing plate 860 and the partitioned flow channel body are connected. The fixed connection of 841 fixes the sealing ring 850 to the partitioned flow channel body 841. At the same time, the T-shaped straight part of the sealing ring 850 protrudes out of the sealing pressure plate 860. When the sealing flow channel device 840 is pressed against the solid phase chip 811 to be pressed, the protruding part of the T-shaped straight part of the sealing ring 850 after protruding out of the sealing pressure plate 860 is tightly attached to the surface of the chip 811. The surface of the chip 811 is divided into several sealed reaction chambers 812 by several sub-rings 851.

[0320] The height of the sub-rings 851 protruding from the sealing plate 860 is set to H, where 0.3mm ≤ H ≤ 0.35mm. This dimension refers to the natural state. When pressed with the chip, the height of the sub-rings 851 protruding from the sealing plate 860 is 0.1 ± 0.02mm, which is the liquid film height. The number of sealed reaction chambers 812 is the same as the number of sub-rings 851.

[0321] In this embodiment, the first layer of protrusions 843 is preferably disposed at the center of the partitioned flow channel body 841; a plurality of second layer protrusions 844 are disposed with the same shape and area; a plurality of sub-rings 851 are disposed with the same shape and area; the length and width of the sealing plate 860 are consistent with the length and width of the partitioned flow channel body 841; the area of ​​the sealing plate 860 is matched with the area of ​​the chip 811 to be pressed; the consistency of the flow channel state is more conducive to the stability of fluid control, thereby ensuring that the set reaction is completed according to the process requirements.

[0322] In this embodiment, seven independent sealed reaction chambers 812 are preferred, and the sub-channel reaction zones are separated by partitioned sealing rings 850. The sealed channel structure essentially divides a large reaction zone into seven smaller reaction zones, making it easier to control processes such as fluid injection, purging, and degassing.

[0323] Referring to Figure 30, the microfluidic device 800 also includes a gas-liquid tube lifting device 890 fixedly connected to the pressure plate assembly, which is used to move synchronously with the pressure plate assembly to avoid bending damage to the gas and liquid tubes during long-distance movement of the mechanism, thereby improving sealing and service life.

[0324] Specifically, the gas-liquid pipe lifting device 890 includes a bridging plate 891, a gas source delivery pipe, a waste liquid collection pipe, a first hollow pipe 892 accommodating the gas source delivery pipe, a second hollow pipe 893 accommodating the waste liquid collection pipe, and a lifting drag chain 894; the bridging plate 891 is fixedly connected to the side of the pressure equalizing plate 836; the first hollow pipe 892 and the second hollow pipe 893 are both fixedly connected to the bridging plate 891; one end of the lifting drag chain 894 is fixedly mounted to the side of the bracket 821, and the other end is fixedly connected to the bridging plate 891, and rises and falls together with the bridging plate 891; some cables are mainly arranged inside the lifting drag chain 894.

[0325] In this embodiment, a first hollow tube 892 is fitted into a first linear bearing, which is mounted on a first linear bearing mounting plate 824. The first linear bearing mounting plate 824 is connected and fixedly connected to a bridging plate 891. A second hollow tube 893 is fitted into a second linear bearing, which is mounted on a second linear bearing mounting plate 824. The second linear bearing mounting plate 824 is connected and fixedly connected to the bridging plate 891. In this embodiment, the first hollow tube 892 and the second hollow tube 893 can slide up and down in the first linear bearing and the second linear bearing, respectively.

[0326] The first hollow tube 892 is equipped with an air inlet connector and an air outlet connector at both ends, and the second hollow tube 893 is equipped with a liquid pipe connector at both ends. The air inlet connector, air outlet connector, and liquid pipe connector are all fixedly connected to the bridge plate 891.

[0327] The gas-liquid switching unit is connected to the waste liquid collection pipe via pipes and connectors.

[0328] In this embodiment, there are two first hollow tubes 892, with air inlet connectors at the bottom and air outlet connectors at the top, providing an air source for high-pressure purging of the liquid circuit system; there are two second hollow tubes 893, with liquid circuit connectors connected to the top and bottom of the two second hollow tubes 893, and the liquid circuit connectors are then connected to the bridge plate 891; the waste liquid pipe coming out of the sealed flow channel structure passes directly through the two second hollow tubes 893 and connects to the subsequent waste liquid collection unit.

[0329] Furthermore, the gas-liquid switching unit includes multiple valves and solenoid valves, enabling rapid switching, cleaning, and purging of various reagents without cross-contamination, ensuring the reaction is completed according to process requirements. Different process flows can be achieved by setting different process parameters, ensuring process flexibility. The liquid system also features comprehensive functions such as liquid preparation, waste liquid collection, and liquid cleaning.

[0330] In this implementation, by analyzing the physicochemical properties of various reaction reagents and conducting extensive resistance tests, suitable corrosion-resistant materials were selected for the components in contact with the reagents to ensure the safety and stability of the liquid circuit system.

[0331] In this embodiment, the liquid circuit system can deliver different reagents and gases at different pressures to the sealed flow channel structure and the sealed reaction chamber formed after chip bonding, thereby completing processes such as reagent filling, reagent reaction, and rinsing. Switching between different reagents and gases is accomplished through a multi-way valve in the gas-liquid circuit switching unit, and the switching process is free of cross-contamination and has negligible trace residual contamination. Liquid supply is achieved by filling the reagent bottle with positive pressure inert gas to force the reagent out. Waste liquid collection utilizes standardized waste liquid collection tanks, with all waste liquid and waste gas pipelines integrated into the tank lid; replacing the waste liquid tank only requires replacing the tank body. Furthermore, by setting different process parameters, different process flows can be achieved, ensuring process flexibility.

[0332] In this implementation, the gantry structure serves as the basic framework of the microfluidic cleaning system, possessing functions such as high rigidity, corrosion resistance, and adjustable level. The main purpose of level adjustment is to ensure that after the sealed flow channel structure is pressed with the chip, the seal is uniform to prevent leakage, while forming a reaction liquid film of the same thickness to ensure the consistency of reaction and rinsing.

[0333] It should be noted that this application can achieve independent control of each sealed reaction chamber, or several of them can be controlled synchronously. For example, if there are a total of 7 sealed reaction chambers, 4 can be controlled as a group and the remaining 3 can be controlled as a group. Zoned control can better realize the fluid function. These are all within the protection scope of this application, so they will not be elaborated here. In addition, for waste liquid collection, low-pressure or high-pressure purging gas can be provided to the sealed reaction chamber to discharge the liquid in the chamber through the liquid pumping pipeline assembly.

[0334] In this embodiment, the microfluidic device and the third-party directional drive device constitute a microfluidic cleaning system for DNA chip synthesis. The carrier device is connected to the carrier device through a horizontal adjustment component, giving the carrier device a degree of freedom for horizontal adjustment. The pressure plate lifting device achieves precise lifting and lowering of the pressure plate through a power component, ensuring a consistent sealing effect when processing chips of different sizes and shapes, thereby improving reaction efficiency and consistency. The stepped protrusions of the partitioned flow channel body are matched with the sealing ring to ensure the sealing of the reaction chamber. Several sub-rings of the sealing ring protrude from the sealing pressure plate, further enhancing the sealing effect, preventing fluid leakage, and ensuring the reliability and efficiency of the cleaning and reaction process. The protruding part of the sealing ring and... The pressed solid-phase chips form a sealed reaction chamber. This design effectively isolates different reaction areas, prevents cross-contamination between different reactions, and improves the purity of the reaction and the accuracy of the results. The liquid system includes a liquid supply unit, a gas supply unit, and a waste liquid collection unit, which are connected to each sealed reaction chamber through their respective pipeline assemblies. This not only enables precise control of different reaction steps but also simplifies the cleaning and waste disposal process, effectively improving the overall efficiency and ease of operation of the system. Through the separately connected liquid supply pipeline assembly, gas supply pipeline assembly, and liquid extraction pipeline assembly, independent control of multiple reaction chambers can be achieved, adapting to the synthesis needs of high-throughput and large-size chips and meeting the requirements of complex DNA synthesis reactions.

[0335] In this embodiment, the microfluidic device divides a large reaction area into several independent small reaction areas (i.e., several sealed reaction chambers) through partitioned sealing and partitioned fluid control. Matching chips form openable and closable reaction chambers, which can control different reagents to complete the required reaction process in the corresponding sealed reaction chambers. At the same time, it can control the formation of a small liquid film thickness. After the reaction is completed, the corresponding sealed reaction chambers can be quickly cleaned, effectively solving the problems of uneven liquid injection, incomplete cleaning, and incomplete purging in the large reaction area in the prior art. This makes it easier to realize high-throughput large-size chip synthesis reactions.

[0336] Microfluidic devices divide a large reaction area into several smaller reaction zones (i.e., sealed reaction chambers), and then precisely control the fluid flow within each small reaction zone. Through zoned sealing and fluid control, multi-zone reaction chambers that can be opened and closed can be formed. This allows for the control of different reagents within the reaction chambers to complete the required reaction process and to thoroughly remove waste liquid. The device features a precise and stable structure, highly resistant reagent contact materials, multi-zone control enabling large-area synthetic reactions, and flexible reaction processes. Microfluidic devices, with their precise and stable structure, highly resistant reagent contact materials, multi-zone control enabling large-area synthetic reactions, and flexible reaction processes, play a crucial role in biosynthesis, especially in DNA synthesis.

[0337] Referring to Figures 31 and 32, the solid-phase chip surface biochemical synthesis system disclosed in this application further includes: a synthesis chip information acquisition device installed on the side of the marble support 140. The synthesis chip information acquisition device includes a dual-camera adjustment assembly and two cameras 911 installed on the dual-camera adjustment assembly. The field of view centers of the two cameras coincide with the centers of the two crosshairs of the solid-phase chip, respectively.

[0338] The dual-camera adjustment assembly also has two sets of light source assemblies 912 corresponding to the two cameras 911.

[0339] In this embodiment, the chip to be tested has the following specifications: external dimensions of 200x200mm and crosshair center distance of 170mm. Through the dual-camera adjustment component disclosed in this application, the field of view centers of the two cameras 911 can be made to almost completely coincide with the center of the crosshair.

[0340] Referring to Figures 32 and 33, the dual-camera adjustment assembly includes an assembly support plate 920, a Z-axis displacement stage 930, a turntable 940, a support rod 960, an adapter plate 950, a vertical support plate 970 for mounting the camera 911, and a joint bearing unit 980. The Z-axis displacement stage 930 is mounted on the side of the assembly support plate 920, and the turntable 940 is mounted on the side of the Z-axis displacement stage 930 away from the assembly support plate 920. That is, one side of the Z-axis displacement stage 930 is fixedly connected to the assembly support plate 920, and the other side (i.e., the outer side) has a degree of freedom of lifting along the Z direction (i.e., a degree of freedom of movement parallel to the Z-axis direction), which can drive the lifting of the turntable 940 fixed on the outer side.

[0341] A transition plate 950 is installed on the side of the turntable 940 away from the Z-axis displacement stage 930. The transition plate 950 is fixedly connected to the side of the turntable 940 through several support rods 960, and the transition plate 950 and the turntable 940 form an accommodating space. The outer side of the turntable 940 has a degree of freedom to rotate around the X-axis, and the transition plate 950 can be driven to rotate around the X-axis through the support rods 960.

[0342] The Z-axis displacement stage 930 includes a first plate 931 fixed to the side of the assembly support plate 920, a first guide rail fixedly mounted on the first plate 931, a second plate 932 (i.e., a sliding plate), a second guide rail fixedly mounted on the second plate 932, an adjusting knob 933, and a locking member 934. The first and second guide rails form a cross roller guide rail structure, and the longitudinal axis of the cross roller guide rail structure is parallel to the Z-axis. The second guide rail has a degree of freedom to move relative to the first guide rail. The locking member 934 has a degree of freedom to lock the second guide rail. The second plate 932 can be moved by controlling the adjusting knob 933 (i.e., the fine-tuning knob), and locked by the locking member 934 (i.e., the locking screw). The adjusting knob 933 and the second plate 932 are separate structures, and force can only be applied in one direction. When rotating, the second plate 932 is reset by a spring.

[0343] Furthermore, the flatness of the second plate 932 is preferably around 5 micrometers.

[0344] The turntable 940 is an L / R manual rotary slide, capable of 360° coarse adjustment and ±5° fine adjustment. Specifically, the turntable contains precision copper bushing bearings. When the coarse adjustment is released, the turntable can rotate 360 ​​degrees. After the coarse adjustment, fine adjustment (±5°) can be performed using the fine adjustment knob. After fine adjustment, it is locked in place by the opposing locking mechanism.

[0345] The side of the manual rotating slide away from the second plate 932 is parallel to the Z-axis and has a degree of rotational freedom. Specifically, the side of the manual rotating slide away from the second plate 932 has a degree of rotational freedom about a direction perpendicular to the second plate 932 (i.e., about the X-axis).

[0346] Among them, several support rods 960 are arranged in parallel and perpendicular to the side of the turntable 940 away from the Z-direction displacement stage 930, ensuring that the plane of the adapter plate 950 is parallel to the outer side of the turntable 940.

[0347] Furthermore, at least four support rods 960 are provided to ensure the installation stability of the outer side of the adapter plate 950 and the turntable 940.

[0348] When four support rods 960 are provided, the support rods 960 include a first rod 961, a second rod 962, a third rod 963 and a fourth rod 964. The center line connecting the first rod 961 and the second rod 962 coincides with the diameter of the turntable 940. The third rod 963 and the fourth rod 964 are respectively provided corresponding to the first rod 961 and the second rod 962.

[0349] In this embodiment, the adapter plate 950 has a plurality of elongated slots that match a plurality of support rods 960; the longitudinal axis of the elongated slots is parallel to the first horizontal direction (e.g., the Y-axis), and the support rods 960 have the degree of freedom to move along the longitudinal axis of the elongated slots.

[0350] The longitudinal axis of the support rod 960 is parallel to the second horizontal direction (e.g., the X-axis), and the second horizontal direction is perpendicular to the first horizontal direction.

[0351] Since the support rod 960 is fixedly connected to the outer side of the turntable 940, the adapter plate 950 can move freely in the Y direction relative to the turntable 940 through several long slots, that is, it has a degree of freedom of movement parallel to the Y-axis.

[0352] A vertical support plate 970 is provided within the accommodating space (i.e., the vertical support plate 970 is located between the adapter plate 950 and the turntable 940). The vertical support plate 970 has a camera mounting part. In this embodiment, the camera mounting part includes a first mounting part and a second mounting part. The first mounting part is provided with a body mounting plate 971 fixed to the body of the camera 911. The second mounting part is provided with a lens limiting plate 972 fixed to the lens of the camera 911, thereby constraining the position of the lens and securely mounting the camera 911. That is, the camera 911 and the vertical support plate 970 form a whole and move synchronously.

[0353] A number of spherical bearing units 980 are mounted on the vertical bearing plate 970; a number of adjustment holes are opened on the adapter plate 950, and the free ends of the spherical bearing units 980 are respectively connected to the adjustment holes, and each spherical bearing unit 980 has a degree of freedom of movement relative to the adapter plate 950.

[0354] Specifically, the spherical bearing unit 980 includes a bearing housing fixedly connected to the vertical support plate 970, a spherical bearing installed on the bearing housing, a locking cover installed on one side of the spherical bearing, and an adjusting screw installed on the other side of the spherical bearing. The free end of the adjusting screw is connected to the adapter plate 950 through a locking member 934. The locking member 934 includes a first nut and a second nut respectively provided on both sides of the adapter plate 950. Both the first nut and the second nut are threadedly connected to the adjusting screw.

[0355] In this embodiment, three joint bearing units 980 are preferably provided, and the centers of the three joint bearing units 980 form a right triangle.

[0356] Referring to Figure 34, the three joint bearing units 980 are respectively the first unit 981, the second unit 982, and the third unit 983; the center line connecting the first unit 981 and the second unit 982 is parallel to the center line connecting the first rod 961 and the second rod 962; the distance between the center of the first unit 981 and the center of the second unit 982 is h, and the distance between the center of the first rod 961 and the center of the second rod 962 is H, where H / 2≤h<H, ensuring the load-bearing stability of the vertical bearing plate 970 through the adapter plate 950.

[0357] Based on the principle that three points determine a plane, the vertical support plate 970 can be rotated around the Z-axis by adjusting the length of the adjusting screw in the first unit 981 alone; the vertical support plate 970 can be rotated around the Y-axis by adjusting the length of the adjusting screw in the third unit 983 alone; and the vertical support plate 970 can be moved along a direction parallel to the X-axis by adjusting the lengths of the adjusting screws in the first unit 981, the second unit 982, and the third unit 983 simultaneously.

[0358] The dual-camera adjustment assembly disclosed in this application allows the camera 911 to be moved up and down along the Z-axis via the lifting and lowering motion of the outer side of the Z-axis displacement stage 930. This can be achieved by using the support rod 960, adapter plate 950, joint bearing unit 980, and vertical support plate 970 to adjust the Z-axis position of the camera 911. The Z-axis displacement adjusts the distance between the lens of the camera 911 and the chip surface, thereby adjusting the focal length. The outer side of the turntable 940 has a degree of freedom to rotate around the X-axis. The support rod 960 can drive the adapter plate 950 to rotate around the X-axis, thus allowing the camera 911 to be rotated and adjusted around the X-axis. Several The connection between the support rod 960 and the adapter plate 950 has a degree of freedom of movement parallel to the Y-axis, which means that the camera 911 can be moved and adjusted freely in the direction parallel to the Y-axis. Several joint bearing units 980 can realize the rotational adjustment of the vertical support plate 970 around the Z-axis, the rotational adjustment of the vertical support plate 970 around the Y-axis, and the movement adjustment along the direction parallel to the X-axis, which means that the camera 911 can be rotated and adjusted around the Z-axis, the rotational adjustment of the vertical support plate 970 around the Y-axis, and the movement adjustment along the direction parallel to the X-axis. Through the dual-camera adjustment assembly disclosed in this application, the position adjustment of the mounted camera 911 with six degrees of freedom can be realized.

[0359] In this embodiment, each vision system is matched and installed with the camera mounting part; both vision systems and two light source assemblies 912 are fixedly installed on the vertical support plate 970, the assembly support plate 920 in the two dual-camera adjustment assemblies is the same plate, the height of the two dual-camera adjustment assemblies is the same, and the two light source assemblies 912 are respectively installed on the two vertical support plates 970.

[0360] Furthermore, the center position between the two sets of dual-camera adjustment components is aligned with the center of the assembly support plate 920 to ensure installation stability.

[0361] Since the relative position of the camera 911 to the printing system and the chip is imprecise and uncertain when the camera 911 is installed, the position of the two cameras 911 can be adjusted based on the chip position first, so as to achieve precise adjustment of the relative position of the vision system and the chip, thereby ensuring higher alignment accuracy.

[0362] Based on the aforementioned application scenarios and requirements, in the dual-camera positioning and adjustment component, each camera can independently perform positional adjustment in six degrees of freedom, including X-axis displacement, Y-axis displacement, Z-axis displacement, rotation around the X-axis, rotation around the Y-axis, and rotation around the Z-axis. The size and positional accuracy of the two markers on the chip are at the micrometer level, which is highly precise. Therefore, the relative position of the two cameras 911 can be determined and adjusted by the position of the markers within the field of view of each camera 911. By precisely adjusting and locking the spatial position of the two cameras 911, high-precision visual alignment and high stability can be achieved, effectively reducing printing deviations and improving synthesis efficiency.

[0363] The dual-camera adjustment assembly disclosed in this application has several significant advantages in the field of DNA biosynthesis using high-throughput synthesis chips. Specifically, it provides multiple degrees of freedom adjustment capabilities through the combination of a Z-axis displacement stage, a turntable, and a support rod, ensuring that the camera can be precisely adjusted in the X, Y, and Z axes as well as in the rotational directions around the X, Y, and Z axes. This allows the camera to accurately align with the crosshairs on the chip, improving printing accuracy. The relative position of the printing system and the chip is calculated through a vision system, and then the printing position is calibrated. The multi-degree-of-freedom adjustment ensures that the camera can capture images of the reaction sites from the optimal viewing angle, thereby improving calibration accuracy.

[0364] In this application, the vertical support plate is connected to the adapter plate via a spherical bearing unit, enabling it to rotate around the Z and Y axes and move along the X axis. This allows the camera to be flexibly adjusted according to the design of different chips and the layout of reaction sites, adapting to reaction sites of different sizes and spacings. The accommodating space formed between the adapter plate and the turntable allows the vertical support plate and the camera to be placed in a relatively fixed environment, reducing the influence of the external environment and improving stability and reliability. The multi-degree-of-freedom adjustment capability allows the camera to quickly align with the crosshairs on the chip, reducing calibration time and steps and improving work efficiency. High-precision positioning and calibration reduce synthesis failures or inefficient synthesis caused by positional errors, improving the overall efficiency of the synthesis process.

[0365] This device is particularly suitable for high-throughput chip synthesis. Precise positioning can be achieved simply by aligning the camera's field of view with the crosshairs on the chip. Since the crosshairs and reaction sites on the chip have absolute physical positions during chip processing, the algorithm determines the position of the reaction sites after the camera aligns with the crosshairs. This ensures accurate printing of each reaction site simultaneously, meeting the needs of large-scale production. The device's design allows for adjustments based on different chip sizes and site layouts, offering excellent scalability to adapt to various production scales and types. Multi-degree-of-freedom adjustment and high-precision positioning ensure stability during long-term use, reducing maintenance costs due to error accumulation. The design of the joint bearing unit and support rod allows for easy disassembly and maintenance, lowering repair and replacement costs.

[0366] Referring to Figures 35 and 36, the solid-phase chip surface biochemical synthesis system disclosed in this application also includes a multi-nozzle flexible ink wiping device installed on a marble platform. The multi-nozzle flexible ink wiping device is used to simultaneously wipe multiple independently set single nozzles in the printing device, wherein simultaneous ink wiping refers to wiping multiple independently set single nozzles at the same time.

[0367] Specifically, the multi-nozzle flexible ink wiping device includes: a first support platform 1100 fixedly connected to the marble platform 130, a first drive assembly 1200 installed on the first support platform 1100, and a slider assembly 1300 installed on the power output end of the first drive assembly 1200. The slider assembly 1300 is equipped with a soiled nonwoven fabric tightening assembly 1400, a new nonwoven fabric supply assembly 1500, and an ink wiping assembly 1600. An outward-facing space is provided between the soiled nonwoven fabric tightening assembly 1400 and the ink wiping assembly 1600. A guide shaft 1700 for tightening the nonwoven fabric is provided between the ink wiping assembly 1600 and the new nonwoven fabric supply assembly 1500, and a tensioning shaft 1800 for pressing the nonwoven fabric inward. The center line connecting the guide shaft 1700 and the tensioning shaft 1800 is parallel to the center line connecting the dirty nonwoven fabric tightening assembly 1400 and the new nonwoven fabric supply assembly 1500, and the center line connecting the guide shaft 1700 and the tensioning shaft 1800 is set lower than the ink wiping assembly 1600. The ink wiping assembly 1600 stretches the nonwoven fabric outward to form a flexible ink wiping area that matches the multiple printheads.

[0368] The distance from the top of the flexible ink erasing area to the marble platform is h1, and the distance from the bottom of the printing device to the marble platform is h2, where 3mm ≤ h1 - h2 ≤ 5mm.

[0369] Driven by the first drive assembly 1200, the slider assembly 1300 can be controlled to reciprocate linearly relative to the first support platform 1100, that is, the ink wiping assembly 1600 can be controlled to move linearly to a position close to the multi-printhead, and then the ink wiping assembly 1600 can be controlled to continue to move forward and wipe the multi-printhead through the flexible ink wiping area.

[0370] In this embodiment, in the initial state, the non-woven fabric on the flexible ink-wiping area formed by the ink-wiping assembly 1600 and matched with the multi-printhead is clean. The slider assembly 1300 is moved closer to the multi-printhead by the power drive of the first drive assembly 1200. Then the flexible ink-wiping area will contact the bottom of the multi-printhead. During the continued forward movement, the ink wiping and cleaning of the multi-printhead is completed. Then it continues to move forward and the flexible ink-wiping area will move to a position that is offset from the multi-printhead. Then it will stop by the power drive of the first drive assembly 1200. At this time, because the flexible ink-wiping area is in a position that is offset from the multi-printhead, the power drive of the multi-printhead can be controlled to move the multi-printhead away. After the multi-printhead is moved away, the slider assembly 1300 is moved back to the original position by the power drive of the first drive assembly 1200.

[0371] After completing a multi-printer cleaning and wiping process, the dirty nonwoven fabric tightening component 1400 can be controlled to move the dirty nonwoven fabric in the flexible wiping area without interfering with the multi-printer. This allows the new nonwoven fabric supply component 1500 to provide new nonwoven fabric, ensuring that the nonwoven fabric in the flexible wiping area is new and ready for the next process.

[0372] Specifically, the first drive assembly 1200 includes a first motor 1210 and a first lead screw 1220 installed at the power output end of the first motor 1210; the first motor 1210 is fixedly installed on the first support platform 1100 via a motor mounting bracket 1230.

[0373] The first bearing platform 1100 is provided with two guide rails 1110, and the longitudinal axis of the two guide rails 1110 is arranged parallel to the longitudinal axis of the first lead screw 1220; the bottom of the slider assembly 1300 has grooves that respectively engage and match with the two guide rails 1110.

[0374] The slider assembly 1300 is matched with the first lead screw 1220, that is, under the drive of the first motor 1210, the first lead screw 1220 is controlled to rotate. Through the matching setting with the first lead screw 1220, the slider assembly 1300 is controlled to move linearly, that is, the slider assembly 1300 has the degree of freedom to move along the longitudinal axis of the first bearing platform 1100.

[0375] Specifically, the slider assembly 1300 is fixedly connected to the nut sleeved on the first lead screw 1220. When the first lead screw 1220 rotates in its original position, it will drive the nut to move along the first lead screw 1220, thereby driving the slider assembly 1300 to move.

[0376] The slider assembly 1300 includes a second support platform 1310, a transition plate 1320 fixed to the top of the second support platform 1310, and a vertical support plate 1330 fixed to the side of the transition plate 1320. The vertical support plate 1330 and the transition plate 1320 form an L-shaped structure.

[0377] The soiled nonwoven fabric tightening assembly 1400 includes a winding motor 1410 fixed to a vertical support plate 1330, a drive shaft 1420 installed at the power output end of the winding motor 1410, and a soiled nonwoven fabric roll 1430 fixedly sleeved on the drive shaft 1420. In this embodiment, the new design uses a nonwoven fabric roll as the medium for cleaning the nozzle. Each time the nozzle is cleaned, the drive shaft 1420 rotates at an angle under the control of the winding motor 1410 to retract the soiled fabric roll. The next time the nozzle is wiped, a brand new nonwoven fabric is used.

[0378] The new nonwoven fabric supply assembly 1500 includes a damping device 1510 fixed to a vertical support plate 1330, a driven shaft 1520 matched with the damping device 1510, and a new nonwoven fabric roll 1530 fixedly sleeved on the driven shaft 1520. The direction of resistance of the damping device 1510 to the driven shaft 1520 is opposite to the driving direction of the winding motor 1410 to the drive shaft 1420, ensuring that after the winding motor 1410 stops, the driven shaft 1520 stops precisely under the action of the damping device 1510, ensuring that the nonwoven fabric between the new nonwoven fabric roll 1530 and the dirty nonwoven fabric roll 1430 is always in a taut state.

[0379] The ink wiping assembly 1600 includes a cantilever plate 1610 fixed to a vertical support plate 1330 and a soft rubber pad fixed to the top of the cantilever plate 1610. The cantilever plate 1610 is vertically fixed to the vertical support plate 1330. The longitudinal length of the soft rubber pad is greater than the sum of the widths of multiple independently set single printheads, ensuring simultaneous ink wiping of multiple printheads, which is simple, efficient, and effectively prevents cross-contamination.

[0380] In this embodiment, the top of the flexible ink wiping area is set higher than the bottom of the multi-printhead, preferably 3mm to 5mm higher, because the flexible ink wiping area is flexible, this setting can ensure sufficient contact and cleaning of the bottom of the multi-printhead.

[0381] In this embodiment, the overhanging arrangement of the dirty nonwoven fabric tightening component 1400, the new nonwoven fabric supply component 1500, and the ink wiping component 1600 allows for quick disassembly and assembly from the end when the new nonwoven fabric is used up.

[0382] Referring to Figure 37, the soft rubber pad includes a first long rubber pad 1621 and a second long rubber pad 1622 arranged in parallel. The first long rubber pad 1621 and the second long rubber pad 1622 have the same structure. The first long rubber pad 1621 is arranged away from the tensioning shaft 1800, that is, the first long rubber pad 1621 is arranged close to the guide shaft 1700.

[0383] The following detailed description uses the first long rubber pad as an example. The first long rubber pad 1621 includes a bottom of the rubber pad and a semi-circular rubber pad disposed on the bottom of the rubber pad. The length of the bottom of the rubber pad is less than the length of the suspension plate 1610, which can effectively ensure the stable bearing of the first long rubber pad 1621 by the suspension plate 1610, and at the same time ensure that the semi-circular rubber pad has sufficient wiping force when it comes into contact with the non-woven fabric (i.e., wiping ink).

[0384] The distance between the two semicircular rubber pads in the first long rubber pad 1621 and the second long rubber pad 1622 is no more than 2mm, that is, the first long rubber pad 1621 and the second long rubber pad 1622 can be set with a gap or in contact.

[0385] Among them, the dirty nonwoven fabric between the first long rubber pad 1621 and the guide shaft 1700 forms a first oblique line, the new nonwoven fabric between the second long rubber pad 1622 and the tension shaft 1800 forms a second oblique line, and the new nonwoven fabric between the first long rubber pad 1621 and the second long rubber pad 1622 forms a horizontal line.

[0386] The angle between the first diagonal line and the horizontal line is smaller than the angle between the second diagonal line and the horizontal line. The angle between the second diagonal line and the horizontal line is α0, where 30°≤α0≤60°. This ensures that when the flexible ink-wiping area is staggered from the multi-printer, the multi-printer will not come into contact with the non-woven fabric at the second diagonal line, because the non-woven fabric at the second diagonal line is new, effectively preventing contamination of the new non-woven fabric.

[0387] In this embodiment, the top of the tensioning shaft 1800 is positioned below the first long rubber pad 1621, which can both tension the new section of nonwoven fabric and protect the new section of nonwoven fabric, effectively avoiding contact with multiple nozzles.

[0388] One end of the guide shaft 1700 and the tensioning shaft 1800 are fixedly installed on the vertical bearing plate 1330; the angle between the center line connecting the guide shaft 1700 and the drive shaft 1420 and the center line connecting the driven shaft 1520 and the drive shaft 1420 is α1, 30°≤α1≤60°.

[0389] The angle between the line connecting the centers of tensioning shaft 1800 and driven shaft 1520 and the line connecting the centers of driven shaft 1520 and driving shaft 1420 is α2, where 30°≤α2≤60°.

[0390] The distance H between the center line connecting the guide shaft 1700 and the tensioning shaft 1800 and the center line connecting the dirty nonwoven fabric tightening component 1400 and the new nonwoven fabric supply component 1500 is 40mm≤H≤60mm.

[0391] Furthermore, the side of the first support platform 1100 is provided with a first sensor 1910 and a second sensor 1920 that are matched with the slider assembly 1300. The distance between the first sensor 1910 and the second sensor 1920 is set to correspond to the moving distance of the slider assembly 1300. The first sensor 1910 is the sensor at the origin position, and the second sensor 1920 is the farthest position moved after ink wiping.

[0392] The multi-nozzle flexible ink wiping device disclosed in this application uses non-woven fabric as the wiping material, and each nozzle has an independent wiping area, ensuring that each nozzle only contacts its own wiping material, thereby effectively avoiding cross-contamination problems; the flexible properties of the non-woven fabric also make the wiping process gentler and reduce damage to the nozzles.

[0393] Traditional ink strips are prone to losing their cleaning effectiveness due to corrosion or crystallization from DNA synthetic inks, resulting in incomplete wiping and affecting printhead cleanliness. The multi-printhead flexible ink wiping device disclosed in this application, through the cooperation of a soiled nonwoven fabric tightening component and a new nonwoven fabric supply component, ensures that clean nonwoven fabric is always used for wiping, avoiding the problem of decreased cleanliness caused by contamination of the wiping material. Furthermore, the design of the guide shaft and tension shaft keeps the nonwoven fabric taut throughout the wiping process, enabling more effective removal of ink residue and dirt from the printhead surface, improving wiping efficiency and cleanliness.

[0394] Traditional ink-wiping methods use a narrow strip to clean multiple printheads, making it impossible to perform personalized cleaning for each printhead. Furthermore, the hardness of the strip can damage the printhead surface. The ink-wiping component of the multi-printhead flexible ink-wiping device disclosed in this application can stretch the non-woven fabric outward to form a flexible wiping area that matches multiple printheads. This design can adapt to the shape and position of different printheads, ensuring that each printhead is wiped precisely. This avoids uneven wiping caused by differences in printhead shape or position. The flexible wiping area design also reduces mechanical damage to the printhead surface and extends the printhead's service life.

[0395] Traditional ink wiping methods typically require manual operation, which is inefficient and prone to errors. The multi-nozzle flexible ink wiping device disclosed in this application automates the wiping process by combining a first drive component and a slider component. The slider component can move along the longitudinal axis of the first support platform, driving the ink wiping component to wipe multiple nozzles sequentially. The entire process is efficient and precise, reducing human error and improving production efficiency.

[0396] Traditional ink strips are prone to failure during wiping due to ink corrosion or crystallization, requiring frequent replacement and increasing maintenance costs and time. The multi-nozzle flexible ink wiping device disclosed in this application, through the design of a soiled non-woven fabric tightening component and a new non-woven fabric supply component, allows for convenient replacement of the non-woven fabric. During wiping, the soiled non-woven fabric is tightened, and new non-woven fabric is supplied, ensuring that the wiping material remains clean at all times. This design not only improves wiping efficiency but also reduces maintenance costs and time.

[0397] Traditional rubber strip wiping methods are poorly adaptable to different types of printheads and inks, especially when handling special inks (such as DNA synthesis inks), which can easily lead to incomplete wiping or material damage. The multi-printhead flexible ink wiping device disclosed in this application, through the precise control of flexible non-woven fabric and guide shafts and tension shafts, can adapt to different types of printheads and inks, and is particularly suitable for high-precision and high-requirement fields such as DNA synthesis. The softness and replaceability of the non-woven fabric make the device perform well when handling different inks, and can effectively cope with the challenges posed by corrosive and crystalline inks.

[0398] This multi-nozzle flexible ink-wiping device offers significant advantages in the field of DNA synthesis. By avoiding cross-contamination, improving wiping efficiency and cleanliness, enabling flexible wiping areas, automating operation, facilitating the replacement of non-woven fabrics, and providing strong adaptability, it solves many problems associated with traditional ink-wiping methods in DNA synthesis. This not only improves the accuracy and efficiency of DNA synthesis but also reduces maintenance costs and enhances the reliability and lifespan of the equipment.

[0399] In another embodiment, the top of the soft rubber pad has a first transition zone, a horizontal zone, and a second transition zone arranged in parallel. The horizontal zone is located between the first and second transition zones. The first transition zone is inclined between the horizontal zone and the guide shaft, and the second transition zone is inclined between the horizontal zone and the tensioning shaft. The widths of both the first and second transition zones are smaller than the width of the horizontal zone. This soft rubber pad arrangement ensures sufficient contact with multiple printheads while maintaining smoothness during contact, resulting in a more uniform and cleaner ink wiping process.

[0400] In the solid-phase chip surface biochemical synthesis system disclosed in this application, the specific synthesis method includes: using the solid-phase chip surface as a synthesis carrier, the monomer and activator are sprayed onto the chip surface through a micro-piezoelectric printhead to carry out a coupling reaction; then, through a microfluidic system, deprotection, oxidation and capping are carried out to complete a four-step cycle, and finally a single base synthesis is completed.

[0401] The above description of the disclosed aspects is provided to enable any person skilled in the art to make or use this disclosure. Various modifications to these aspects will be readily apparent to those skilled in the art, and the general principles defined herein may be applied to other aspects without departing from the scope of this disclosure. Therefore, this disclosure is not intended to be limited to the aspects shown herein, but rather to be carried out within the widest scope consistent with the principles and novel features disclosed herein.

[0402] The above description has been given for purposes of illustration and description. Furthermore, this description is not intended to limit the embodiments of this disclosure to the forms disclosed herein. Although numerous exemplary aspects and embodiments have been discussed above, those skilled in the art will recognize certain variations, modifications, alterations, additions, and sub-combinations therein.

Claims

1. A solid-phase chip surface biochemical synthesis system, comprising a sealed cavity, wherein a marble platform is disposed inside the sealed cavity, and a first horizontal driving device is disposed on the marble platform; The power output end of the first horizontal driving device is provided with a chip stage device, which is used to support solid-state chips. The marble support set on the marble platform has a second horizontal direction drive device that is perpendicular to the first horizontal direction on its side. The power output end of the second horizontal drive device is provided with a printing device, which has a degree of freedom to move along the second horizontal direction. The printing device is used to print on the surface of the solid chip carried by the chip stage device. A moisturizing device is installed on the marble platform and matched with the printing device, the moisturizing device being used for condition maintenance of the printing device; A third-direction driving device is arranged across the top of the first horizontal driving device. A microfluidic device matching the chip stage device is installed at the power output end of the third-direction driving device. The microfluidic device has the freedom to move along the third direction. When the microfluidic device presses against the target chip on the chip stage device, it forms several sealed reaction chambers.

2. The solid-phase chip surface biochemical synthesis system according to claim 1, characterized in that, The first horizontal drive device includes two first direction magnetic rails that are parallel and fixed on the marble platform, a first stator winding that is fixedly installed on the marble platform, and a first mover assembly that is matched with the first stator winding. The first mover assembly includes a first linear motor mover and a first mover mounting block. The chip stage device includes: The stage body has a downward-facing chamber, and the top of the chamber has a chip mounting area with several through holes. A platform support is disposed below the platform body; the platform support is connected to the platform body by a rotating pin; an angle adjustment component is provided on the platform support, and the platform body has the freedom to rotate horizontally around the center of the rotating pin under the adjustment of the angle adjustment component. The stage support is provided with a sealing component that matches the chamber; a negative pressure adsorption component is installed below the sealing component, and the negative pressure adsorption component is connected to the chamber through a pipeline; the chamber forms a negative pressure environment facing several through holes under the action of the negative pressure adsorption component, so as to adsorb the chip covered on the chip mounting area. A horizontal support plate is disposed at the bottom of the platform support; the bottom of the horizontal support plate has two sets of first engaging sliders that respectively match the two first direction magnetic rails; The first linear motor actuator is fixed to the bottom of the horizontal support plate by the first actuator mounting block, and the first linear motor actuator is located between the two sets of the first engaging sliders.

3. The solid-phase chip surface biochemical synthesis system according to claim 1, characterized in that, The second horizontal drive device includes two second direction magnetic rails fixedly and parallel to the side of the marble support, a second stator winding fixedly installed with the side of the marble support, and a second mover assembly matched with the second stator winding. The second mover assembly includes a second linear motor mover and a second mover mounting block. The printing device engages with two second-direction magnetic rails via a vertical support plate. The side of the vertical support plate has two sets of second engaging sliders that respectively match the two second-direction magnetic rails. The second linear motor actuator is fixed to the bottom of the vertical support plate via a second actuator mounting block, and the second linear motor actuator is located between the two sets of second engaging sliders. The printing device includes: Nozzle assembly; The visible ink cartridge has an independently configured individual ink loading chamber and an activator ink loading chamber inside. The individual ink loading chamber and the activator ink loading chamber are respectively connected to corresponding interfaces on the printhead device through pipelines. Both the visible ink cartridge and the printhead device are installed in the printing module housing. A single ink filling pipeline device includes a first ink filling component, a first connector, and a second connector. One end of the first connector is connected to the single ink loading chamber, and the other end has a first engaging portion. One end of the second connector has a second engaging portion that matches the first engaging portion, and the other end is connected to the first ink filling component. An activator ink filling pipeline device includes a second ink filling component, a third connector, and a fourth connector. One end of the third connector is connected to the activator ink loading chamber, and the other end has a third engaging portion. One end of the fourth connector has a fourth engaging portion that matches the third engaging portion, and the other end is connected to the second ink filling component. A one-way valve is installed in both the third engaging portion and the first engaging portion. A pressure source device for providing negative pressure that matches the nozzle device.

4. The solid-phase chip surface biochemical synthesis system according to claim 3, characterized in that, The nozzle device includes multiple independently configured single nozzles and a multi-nozzle position adjustment device for adjusting the positions of the multiple single nozzles; The multi-nozzle position adjustment device includes: A nozzle support plate has a groove on it, and the groove has a plurality of nozzle mounting positions; Each of the nozzle mounting positions is provided with a horizontal deflection adjustment component; the horizontal deflection adjustment component includes an elastic element disposed on the first side of the nozzle and an adjustment element disposed on the second side of the nozzle, and the line connecting the center of the adjustment element and the center of the elastic element is set at a preset angle with the longitudinal axis of the nozzle. A horizontal movement adjustment assembly is installed on the nozzle support plate. The horizontal movement adjustment assembly includes a leveling screw and a spring plunger. A first hole for accommodating the leveling screw is opened on one side of the nozzle support plate, and a second hole for accommodating the spring plunger is opened on the other side. The second hole is matched with the first hole. The top of the leveling screw abuts against a third side of the nozzle, and the top of the spring plunger abuts against a fourth side of the nozzle. The leveling screw has the freedom to move along the first hole.

5. The solid-phase chip surface biochemical synthesis system according to claim 4, characterized in that, The moisturizing device includes: A drive assembly has a first slider mounted on its power output end, the first slider having a degree of freedom to move along the longitudinal axis of the power output end of the drive assembly; a second slider is fixed to the side of the first slider. The transmission assembly includes a guide rail that engages with the second slider and a vertical plate for mounting the guide rail. The longitudinal axis of the guide rail is set at a preset angle with the moving direction of the first slider. The guide rail has the freedom to move up or down under the drive of the second slider. A moisturizing ink pad assembly is fixedly installed on the top of the upright plate; the moisturizing ink pad assembly includes an ink-absorbing top plate, the ink-absorbing top plate has a groove, and a moisturizing pad matching the printhead is disposed in the groove; The ink suction assembly includes an ink suction pump and a waste ink storage bottle connected to the ink suction pump. The ink suction pump is connected to the groove. When the printing device is in standby mode and is located directly above the groove, the groove forms a negative pressure environment facing the corresponding printhead under the action of the ink suction pump.

6. The solid-phase chip surface biochemical synthesis system according to claim 5, characterized in that, The drive assembly includes a drive motor and a lead screw mounted on the power output end of the drive motor; the drive motor is mounted on a support plate, and the support plate is fixedly connected to the marble platform; the support plate is provided with a linear guide rail that engages with the bottom of the first slider, and the linear guide rail is arranged parallel to the lead screw; The support plate is provided with a first side plate, a second side plate, a third side plate, a fourth side plate, and a bearing mounting plate. The first side plate, the second side plate, the third side plate, and the fourth side plate are all fixedly installed on the bottom of the bearing mounting plate. The first side plate is arranged adjacent to the second side plate and opposite to the third side plate. The drive motor is mounted on the support plate through the second side plate. The ink absorption assembly is installed on the outside of the fourth side plate. The bottom of the ink-absorbing top plate is provided with several columns; the columns are fitted with shaft support seats that are fixedly installed to the bottom of the ink-absorbing top plate. The top of the bearing mounting plate is equipped with a linear bearing component that matches the column, and the linear bearing component is matched with the shaft support seat.

7. The solid-phase chip surface biochemical synthesis system according to claim 1, characterized in that, The third-party directional drive device includes a bearing device and a pressure plate lifting device mounted on the bearing device. The bearing device includes a support member and a bearing device mounted on the top of the support member. The bearing device and the support member are connected by a horizontal adjustment assembly, and the bearing device has a horizontal adjustment degree of freedom. The pressure plate lifting device includes a power assembly and a pressure plate assembly mounted on the power output end of the power assembly. The pressure plate assembly has a degree of freedom to move up and down along the longitudinal axis of the power output end of the power assembly. The microfluidic device includes a fluid path system and a sealing channel device installed below the pressure plate assembly. The sealing channel device includes a partitioned channel body, a sealing ring, and a sealing pressure plate. The partitioned channel body has stepped protrusions on its side away from the pressure plate assembly, and the sealing ring is matched with the stepped protrusions. The sealing pressure plate is installed around the sealing ring and is fixedly connected to the partitioned channel body. The sealing ring has several sub-rings, and several of the sub-rings protrude from the sealing pressure plate. The protruding portions of the several sub-rings and the pressed solid-phase chip form several sealed reaction chambers. The liquid circuit system includes a liquid supply unit, a gas supply unit, and a waste liquid collection unit. The liquid supply unit is connected to several of the sealed reaction chambers through a liquid supply pipeline assembly. The gas supply unit is connected to several of the sealed reaction chambers through a gas supply pipeline assembly. The waste liquid collection unit is connected to several of the sealed reaction chambers through a liquid extraction pipeline assembly.

8. The solid-phase chip surface biochemical synthesis system according to claim 7, characterized in that, The microfluidic device also includes a gas-liquid pipe lifting device fixedly connected to the pressure plate assembly; The gas-liquid pipe lifting device includes a bridging plate, a gas source delivery pipe, a waste liquid collection pipe, a first hollow pipe accommodating the gas source delivery pipe, a second hollow pipe accommodating the waste liquid collection pipe, and a lifting drag chain. The bridging plate is fixedly connected to the side of the pressure plate assembly; Both the first hollow tube and the second hollow tube are fixedly connected to the bridge plate; One end of the lifting cable chain is fixed to the side of the support member, and the other end is fixedly connected to the bridge plate.

9. The solid-phase chip surface biochemical synthesis system according to claim 1, characterized in that, It also includes a synthetic chip information acquisition device installed on the side of the marble support. The synthetic chip information acquisition device includes a dual-camera adjustment assembly and two cameras installed on the dual-camera adjustment assembly. The field of view centers of the two cameras are respectively aligned with the centers of the two crosshairs of the solid-phase chip. The dual-camera adjustment assembly includes: An assembly support plate is provided with a Z-axis displacement stage on its side. A turntable is provided on the side of the Z-axis displacement stage away from the assembly support plate, and the side of the Z-axis displacement stage away from the assembly support plate has a degree of freedom of movement parallel to the Z-axis direction. The turntable is equipped with a transition plate on the side away from the Z-axis displacement stage, and the side of the turntable away from the Z-axis displacement stage has a degree of freedom to rotate around the X-axis. The adapter plate is fixedly connected to the side of the turntable by several support rods, and the connection between the several support rods and the adapter plate has a degree of freedom of movement parallel to the Y-axis. The adapter plate and the turntable form an accommodating space; the accommodating space is provided with a vertical support plate, the vertical support plate has a camera mounting part, and the vertical support plate is connected to the adapter plate through a plurality of joint bearing units, the plurality of joint bearing units having a degree of freedom to drive the vertical support plate to rotate around the Z-axis, a degree of freedom to rotate around the Y-axis, and a degree of freedom to move along a direction parallel to the X-axis.

10. The solid-phase chip surface biochemical synthesis system according to claim 1, characterized in that, It also includes a multi-nozzle flexible ink wiping device installed on the marble platform, which is used to simultaneously wipe multiple independently configured single nozzles in the printing device. The multi-nozzle flexible ink-wiping device includes: A first load-bearing platform fixedly connected to the marble platform; The first drive component is installed on the first carrier platform; A slider assembly is installed at the power output end of the first drive assembly; the slider assembly has a degree of freedom to move along the longitudinal axis of the first bearing platform; The slider assembly is equipped with a soiled nonwoven fabric tightening assembly, a new nonwoven fabric supply assembly, and an ink wiping assembly. A guide shaft is provided between the soiled nonwoven fabric tightening assembly and the ink wiping assembly to stretch the nonwoven fabric outward. A tensioning shaft is provided between the ink wiping assembly and the new nonwoven fabric supply assembly to press the nonwoven fabric inward. The center line connecting the guide shaft and the tensioning shaft is parallel to the center line connecting the soiled nonwoven fabric tightening assembly and the new nonwoven fabric supply assembly, and the center line connecting the guide shaft and the tensioning shaft is set lower than the ink wiping assembly. The ink wiping assembly stretches the non-woven fabric outward to form a flexible ink wiping area that matches the multiple printheads; The distance from the top of the flexible ink-wiping area to the marble platform is h1, and the distance from the bottom of the printing device to the marble platform is h2, where 3mm ≤ h1 - h2 ≤ 5mm.

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