Biosensor cartridge

Abstract

The present invention relates to a biosensor cartridge comprising: a housing; a frame disposed in the housing; a biosensor that is detachably coupled to one side surface of the frame and senses a biomaterial; a printed circuit board detachably coupled to the other side surface of the frame; a channel formed in the frame and providing a path along which a buffer solution or a sample solution flows; a sealer disposed between the frame and the biosensor; and a contact clip penetrating the frame to contact the biosensor and the printed circuit board. Therefore, when a defect occurs in the biosensor, only the biosensor can be replaced after separating only a sensor cover.

Description

Biosensor cartridge

[0001] The present invention relates to a biosensor cartridge, and more specifically, to a biosensor cartridge that detects biological material and generates an electrical signal.

[0002] With the recent advancement of science and technology and the growing interest in the quality of life, the importance of disease diagnosis and prevention, as well as food and the environment, in human life is expanding day by day. As a result, there is an increasing need to measure the concentration of organic or inorganic substances in samples to diagnose human diseases, for specific processes in the fields of food chemistry and industrial chemistry, or to analyze pollutants in the environmental field, and many efforts are being made to address this.

[0003] A biosensor refers to a device that measures concentration by binding biomaterials, such as enzymes, microorganisms, antibodies, receptors, and DNA probes, to electrical or physicochemical transducers and detecting signals through electrochemical, optical, thermal, or piezoelectric methods based on electrode active substances or physical changes generated from reactions with the analyte to be measured.

[0004] Generally, the detection of biomaterials using biosensors is achieved through a combination of complex processes such as filtering, metering, mixing, transport, reaction, and washing. Therefore, conventionally, the detection of biomaterials is carried out manually at the laboratory level using various equipment.

[0005] Therefore, alongside the advancement of biosensor technology, the simultaneous development of fluid processing technology to automate and standardize the diagnostic process is crucial for low-cost, high-efficiency diagnosis.

[0006] Among these, equipment for detecting biomaterials requires flushing the internal flow paths before measuring each biological sample. Additionally, since large volumes of buffer solution must be stored in tanks to diagnose multiple samples, there is a risk of degradation, and there is the inconvenience of having to periodically empty the waste solution after diagnosis. Using such equipment presents limitations, as it may lead to reduced diagnostic speed and accuracy when a large volume of samples is required.

[0007] To solve this, a disposable biosensor cartridge with a built-in flow path through which buffer solution and sample solution can flow can be used. Since the disposable biosensor cartridge is used only once, a separate cleaning process is unnecessary. Additionally, since a single-use buffer solution is provided for each cartridge, there is no possibility of the buffer solution deteriorating, and the waste solution is emptied along with the cartridge when it is discarded.

[0008] Meanwhile, in the case of conventional biosensor cartridges, the biosensor and the printed circuit board (PCB) were combined inside the cartridge. Consequently, if a defect occurred in the biosensor during the cartridge manufacturing process, there was the inconvenience of having to disassemble the entire cartridge to replace the sensor.

[0009] In addition, even when reusing the cartridge, there was inconvenience because the cartridge had to be disassembled, the biosensor replaced, and then reassembled.

[0010] The present invention was created to improve upon the problems described above, and aims to provide a biosensor cartridge that allows a user to easily diagnose biomaterials through a simple process.

[0011] In addition, the purpose is to provide a biosensor cartridge that enables easy diagnosis of biomaterials at the site where diagnosis is required, without spatial constraints.

[0012] In addition, the purpose is to provide a biosensor cartridge that allows for diagnosis without spatial constraints and facilitates easy transport to sites requiring diagnosis by configuring the diagnostic device in a compact size.

[0013] In addition, the purpose is to provide a biosensor cartridge capable of stably controlling the flow rate and velocity of a buffer solution or sample solution flowing within the cartridge.

[0014] In addition, the purpose is to provide a biosensor cartridge that can prevent the decrease in sensing accuracy caused by inconsistent solution flow rates for each diagnostic biosensor cartridge.

[0015] In addition, the purpose is to provide a biosensor cartridge that can stably maintain the flow of a buffer solution or sample solution and stably transmit electrical signals while miniaturizing the size of the biosensor.

[0016] In addition, the purpose is to provide a biosensor cartridge that allows only the biosensor to be easily replaced without disassembling the entire cartridge in the event of a defect in the biosensor.

[0017] In addition, the purpose is to provide a biosensor cartridge that allows for easy injection of a buffer solution and prevents contamination of the buffer solution.

[0018] In addition, the purpose is to provide a biosensor cartridge capable of automatically flowing a buffer solution or sample solution into a biosensor and detecting biomaterials through the biosensor with just a simple insertion process into a diagnostic device.

[0019] To solve the problem described above, the biosensor cartridge according to the present invention comprises: a housing; a frame disposed within the housing; a biosensor detachably coupled to one side of the frame and detecting a biomaterial; a printed circuit board detachably coupled to the other side of the frame; a channel formed in the frame and providing a path for the buffer solution or the sample solution to flow through; a sealer disposed between the frame and the biosensor; and a contact clip penetrating the frame and contacting the biosensor and the printed circuit board.

[0020] At this time, the frame may include a sensor coupling part to which the biosensor is detachably coupled.

[0021] In addition, the housing may be formed to allow the biosensor to pass through, and a sensor input hole may be formed at a position facing the sensor coupling part.

[0022] At this time, the diameter of the sensor insertion hole may be larger than the diameter of the sensor coupling part.

[0023] Meanwhile, at least a portion of the side wall surrounding the sensor input hole may have a pair of cover coupling portions formed protruding radially inward.

[0024] Additionally, the sensor cover may include a hook formed protruding from the upper surface of the sensor cover and formed along the circumferential direction for a predetermined angle range.

[0025] At this time, the hook can be coupled to the cover coupling part according to the rotation of the sensor cover.

[0026] Meanwhile, the sensor cover further includes a sensor support member protruding from the upper surface of the sensor cover; and when the sensor cover is coupled to the housing and blocks the sensor insertion hole, the sensor support member can contact and support the biosensor.

[0027] As explained above, the biosensor cartridge according to the present invention is equipped with a valve part and a pump part, thereby providing the effect of automatically diagnosing biomaterials through a simple process of insertion into a diagnostic device.

[0028] In addition, since the valve and pump units are embedded in the biosensor cartridge, it is possible to detect biomaterials by operating the valve and pump units without spatial constraints when necessary.

[0029] In addition, since the valve and pump sections are embedded in the biosensor cartridge and the diagnostic device is equipped with only a simple actuator, the device can be configured compactly for easy transport and effectively eliminate spatial constraints on the installation location.

[0030] In addition, when the pump unit rotates, it presses the tube, which has the effect of stably flowing the buffer solution or sample solution.

[0031] In addition, using a graphene-based biosensor offers the advantage of enabling both miniaturization and sensitive sensing.

[0032] In addition, by forming a hydrophilic adhesive layer using a hydrophilic tape between the base frame and the top frame, the flow rate of the solution can be maintained at a constant level regardless of the environment in which the diagnosis is performed.

[0033] In addition, the purpose is to provide a biosensor cartridge that can stably maintain the flow of a buffer solution or sample solution and stably transmit electrical signals while miniaturizing the size of the biosensor.

[0034] In addition, if a defect occurs in the biosensor, it has the effect of allowing only the biosensor to be replaced after removing only the sensor cover.

[0035] In addition, it has the effect of allowing the user to easily attach the sensor cover and intuitively recognize and perform the method of locking or unlocking the attachment state.

[0036] In addition, by providing a buffer blister, the buffer solution can be injected by simply bursting the sealed buffer blister, and the buffer solution can be prevented from being contaminated.

[0037] In addition, the rotation of the valve section has the effect of automatically flowing the buffer solution and sample solution sequentially.

[0038] FIG. 1 is a drawing for explaining a biosensor cartridge and a diagnostic device according to one embodiment of the present invention.

[0039] FIG. 2 is a perspective view illustrating a biosensor cartridge according to one embodiment of the present invention.

[0040] Figure 3 is an exploded perspective view of Figure 2.

[0041] Figure 4 is a plan view of Figure 2.

[0042] FIG. 5 is a plan view illustrating the state in which the upper housing has been removed from FIG. 4.

[0043] FIG. 6 is an exploded perspective view for explaining a frame according to one embodiment of the present invention.

[0044] FIG. 7 is a plan view for explaining a top frame according to one embodiment of the present invention.

[0045] FIG. 8 is a cross-sectional view of a frame according to one embodiment of the present invention.

[0046] FIG. 9 is a bottom view illustrating a channel formed in a top frame according to one embodiment of the present invention.

[0047] FIG. 10 is a diagram illustrating the combination of a biosensor and a printed circuit board to a frame in a biosensor cartridge according to one embodiment of the present invention.

[0048] FIG. 11 is a drawing for explaining a sensor coupling portion in a biosensor cartridge according to one embodiment of the present invention.

[0049] FIG. 12 is a diagram illustrating the state in which a biosensor is coupled in a biosensor cartridge according to one embodiment of the present invention.

[0050] FIG. 13 is a cross-sectional view illustrating the contact between a biosensor and a printed circuit board in a biosensor cartridge according to one embodiment of the present invention.

[0051] FIGS. 14 and 15 are cross-sectional views illustrating the arrangement of a biosensor and a printed circuit board and the arrangement of a flow path flowing over the biosensor in a biosensor cartridge according to one embodiment of the present invention.

[0052] FIGS. 16 and 17 are drawings for explaining the process of opening and closing a sensor cover in a biosensor cartridge according to one embodiment of the present invention.

[0053] FIG. 18 is an exploded perspective view for explaining the valve part and the pump part in a biosensor cartridge according to one embodiment of the present invention.

[0054] FIG. 19 is an exploded perspective view for explaining the valve portion in a biosensor cartridge according to one embodiment of the present invention.

[0055] FIG. 20 is a perspective view illustrating a channel plate of a valve portion in a biosensor cartridge according to one embodiment of the present invention.

[0056] FIG. 21 is a bottom view illustrating a pump portion in a biosensor cartridge according to one embodiment of the present invention.

[0057] FIG. 22 is a diagram illustrating the process of compressing a tube according to the rotation of a pump part in a biosensor cartridge according to one embodiment of the present invention.

[0058] FIG. 23 is a diagram illustrating the process of introducing a buffer solution as a buffer blister bursts in a biosensor cartridge according to one embodiment of the present invention.

[0059] Preferred embodiments of the present invention will be described in detail below with reference to the attached drawings.

[0060] The present invention is capable of various modifications and may have various embodiments, and specific embodiments are illustrated in the drawings and described in detail in the detailed description. This is not intended to limit the present invention to specific embodiments, and should be interpreted to include all modifications, equivalents, and substitutions that fall within the spirit and scope of the invention.

[0061] In describing the present invention, terms such as "first," "second," etc., may be used to describe various components, but said components may not be limited by said terms. Such terms are used solely for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be named the second component, and similarly, the second component may be named the first component.

[0062] The term "and / or" may include a combination of multiple related listed items or any of the multiple related listed items.

[0063] When it is stated that one component is "connected" or "connected" to another component, it can be understood that while it may be directly connected or connected to that other component, there may also be other components in between. On the other hand, when it is stated that one component is "directly connected" or "directly connected" to another component, it can be understood that there are no other components in between.

[0064] The terms used in this application are used merely to describe specific embodiments and are not intended to limit the invention. Singular expressions may include plural expressions unless the context clearly indicates otherwise.

[0065] In this application, terms such as “comprising” or “having” are intended to indicate the existence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not excluding in advance the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

[0066] Unless otherwise defined, all terms used herein, including technical or scientific terms, may have the same meaning as generally understood by those skilled in the art to which the present invention pertains. Terms such as those defined in commonly used dictionaries may be interpreted as having a meaning consistent with their meaning in the context of the relevant technology, and may not be interpreted in an ideal or overly formal sense unless explicitly defined in this application.

[0067] In addition, the following embodiments are provided to explain more completely to those with average knowledge in the industry, and the shapes and sizes of the elements in the drawings may be exaggerated for clearer explanation.

[0068]

[0069] FIG. 1 illustrates a diagram for explaining a biosensor cartridge and a diagnostic device according to an embodiment of the present invention, FIG. 2 to FIG. 4 illustrates a diagram for explaining a biosensor cartridge according to an embodiment of the present invention, and FIG. 5 illustrates a plan view for explaining the state in which the upper housing in FIG. 4 has been removed.

[0070] For reference, in the present invention, target materials are biomaterials representing a specific substrate and can be interpreted as having the same meaning as analytes. In the present invention, probe materials are biomaterials that specifically bind to target materials and can be interpreted as having the same meaning as receptors or acceptors. In this embodiment, biomaterials may be antigens, antibodies, DNA, small molecule organics, proteins, peptides, amino acids, ligand proteins, etc.

[0071]

[0072] Referring to FIGS. 1 to 5, a biosensor cartridge (1) according to one embodiment of the present invention is described as follows.

[0073] A biosensor system according to one embodiment of the present invention includes a biosensor cartridge (1) and a diagnostic device (2). The biosensor cartridge (1) according to one embodiment of the present invention is coupled to the diagnostic device (2) to detect biomaterials and thereby diagnose diseases, etc.

[0074] At this time, the biosensor cartridge (1) of the present invention can be inserted into the diagnostic device (2) in a state arranged along the horizontal direction. Additionally, the biosensor cartridge (1) can flow buffer solution and sample solution inside to cause the biosensor (400) to undergo an electrochemical reaction with the biomaterial, and can transmit the resulting electrical change to the diagnostic device (2) through the printed circuit board (500).

[0075] Specifically, the biosensor cartridge (1) includes a housing (100), a frame (200), a tank (300), a biosensor (400), a printed circuit board (500), a channel (600), a tube (700), a valve section (800), and a pump section (900).

[0076] At this time, a frame (200) is accommodated inside the housing (100), a tank (300) is formed on the upper side of the frame (200), and a channel (600) may be formed inside the frame (200). Additionally, a tube (700), a valve section (800), and a pump section (900) are coupled to the frame (200), and a biosensor (400) and a printed circuit board (500) may be detachably coupled.

[0077] For reference, in the present invention, the direction in which the buffer solution is introduced relative to the frame (200) can be called the upper side, and the direction opposite to the upper side relative to the frame (200) can be called the lower side. Additionally, the direction in which the biosensor cartridge (1) is inserted into the diagnostic device (2) can be called the front. That is, the direction in which the printed circuit board (500) is placed relative to the housing (100) can be called the front. Additionally, the direction opposite to the front can be called the rear. Furthermore, when looking forward from the rear end of the housing (100), the direction placed on the left can be called the left side, and the direction placed on the right can be called the right side.

[0078] The housing (100) forms the outer shape of the biosensor cartridge (1) and can accommodate a frame (200), a tank (300), a biosensor (400), a printed circuit board (500), a channel (600), a tube (700), a valve section (800), and a pump section (900) inside.

[0079] The housing (100) includes an upper housing (110) and a lower housing (120).

[0080] For example, the upper housing (110) may be formed in the shape of a square box with the lower side open, and a buffer solution inlet hole (111), a sample solution inlet hole (112), a valve connection hole (113), and a pump connection hole (114) may be formed on the upper side.

[0081] The buffer solution inlet hole (111) can be formed to allow the buffer solution to flow in. The buffer solution inlet hole (111) can be formed in the blister receiving groove (111a). At this time, the blister receiving groove (111a) can be positioned vertically above the buffer solution tank (310).

[0082] A buffer blister (3) can be coupled to the blister receiving groove (111a). The blister receiving groove (111a) may be formed by being recessed downward on the upper side of the upper housing (110) in correspondence with the shape of the buffer blister (3). Additionally, at least one blister punch (111d) for piercing the buffer blister (3) may be formed protruding from the blister receiving groove (111a).

[0083] With this configuration, when the buffer blister (3) is pressurized while being received inside the blister receiving groove (111a), the blister punch (111d) bursts the buffer blister (3), allowing the buffer solution to pass through the buffer solution inlet hole (111) and flow into the buffer solution tank (310).

[0084] Meanwhile, according to an embodiment, at least one ventilation hole (111b, 116) may be formed on the upper surface of the upper housing (110). The ventilation hole (111b, 116) may be formed so that air inside the housing (100) can be discharged to the outside.

[0085] Specifically, a first ventilation hole (111b) may be formed on the upper surface of the upper housing (110). At this time, the first ventilation hole (111b) may be positioned vertically above the buffer solution tank (310). Accordingly, the first ventilation hole (111b) may be formed to communicate with the internal space of the buffer solution tank (310).

[0086] Additionally, according to an embodiment, a first ventilation channel (111c) may be further formed on the upper surface of the upper housing (110). The first ventilation channel (111c) may be formed to communicate with the first ventilation hole (111b) and may be formed in a groove shape along the upper surface of the upper housing (110).

[0087] The sample solution inlet hole (112) can be formed to allow the sample solution to flow in. The sample solution inlet hole (112) can be positioned vertically above the sample solution tank (320). Thus, the sample solution inlet hole (112) can be formed to communicate with the internal space of the sample solution tank (320). Meanwhile, a stopper (112a) can be detachably connected to the sample solution inlet hole (112).

[0088] The valve connection hole (113) may be formed so that a part of the valve portion (800) passes through it. The valve connection hole (113) may be positioned on the upper side of the valve coupling portion (223). At this time, the valve connection hole (113) may be formed in the shape of a circular hole, and a valve knob (820) may be positioned to pass through the valve connection hole (113). The valve knob (820) may rotate within the valve connection hole (113).

[0089] The pump connection hole (114) may be formed so that a part of the pump section (900) passes through it. The pump connection hole (114) may be positioned above the valve connection section (223). Specifically, the pump connection hole (114) may be positioned above the pump receiving groove (222a). At this time, the pump connection hole (114) may be formed in the shape of a circular hole, and the pump knob (910) may be positioned to pass through the valve connection hole (113). The pump knob (910) may rotate within the pump connection hole (114).

[0090] Meanwhile, a support projection (115) may be formed protruding from the upper surface of the upper housing (110) to support the printed circuit board (500) into the interior of the housing (100). The support projection (115) may be positioned vertically above the substrate coupling portion (221) of the frame (200). The support projection (115) may contact the upper surface of the printed circuit board (500) to support the printed circuit board (500).

[0091] Meanwhile, according to an embodiment, a second ventilation hole (116) may be further formed on the upper surface of the upper housing (110). In this case, the second ventilation hole (116) may be positioned vertically above the waste solution tank (330). Accordingly, the second ventilation hole (116) may be formed to communicate with the internal space of the waste solution tank (330).

[0092] Additionally, according to an embodiment, a second ventilation channel (116a) may be further formed on the upper surface of the upper housing (110). The second ventilation channel (116a) is formed to communicate with the second ventilation hole (116) and may be formed in a groove shape along the upper surface of the upper housing (110).

[0093]

[0094] The lower housing (120) can be combined with the upper housing (110) to form a space that accommodates a frame (200), a tank (300), a biosensor (400), a printed circuit board (500), a channel (600), a tube (700), a valve section (800), and a pump section (900) inside.

[0095] For example, the lower housing (120) may be formed in the shape of a square box with an open top and may be combined with the upper housing (120). At this time, a plurality of hooks may be formed protruding toward the upper housing (110) on the side wall of the lower housing (120) and may be inserted into a groove (not shown) formed on the side wall of the upper housing (110) to be combined.

[0096] At this time, the attachment between the upper housing (110) and the lower housing (120) can be further strengthened by performing fusion or adhesion on the edge attachment area of ​​the upper housing (110) and the lower housing (120).

[0097] Meanwhile, a sensor insertion hole (121) into which a biosensor (400) can be inserted may be formed on the lower surface of the lower housing (120). At this time, the diameter of the sensor insertion hole (121) may be larger than the diameter of the sensor coupling part (211) to be described later. The cover coupling part (121a) may be coupled with and supported by the hook (122a) of the sensor cover (122).

[0098] Additionally, a sensor cover (122) may be attached to the lower side of the lower housing (120). The sensor cover (122) can cover the sensor insertion hole (121) and prevent the biosensor (400) attached to the frame (200) from detaching.

[0099] The sensor cover (122) includes a hook (122a), a sensor support (122b), and a coupling guide groove (122c). For example, the sensor cover (122) may be formed in the shape of a disc overall, with the hook (122a) and the sensor support (122b) protruding from the upper surface of the sensor cover (122), and the coupling guide groove (122c) recessed from the lower surface of the sensor cover (122). At this time, the diameter of the sensor cover (122) may be formed to correspond to the diameter of the sensor insertion hole (121). For example, the diameter of the sensor cover (122) may be formed to be the same as the diameter of the sensor insertion hole (121).

[0100] The hook (122a) is formed to protrude upward from the upper surface of the sensor cover (122), and the upper portion may be formed to protrude radially outward. For example, a pair of hooks (122a) may be formed in a position facing each other and may be formed along a predetermined angle range along the circumferential direction. With this configuration, the hook (122a) can be positioned above the cover coupling part (121a) when the sensor cover (122) is rotated after being inserted into the sensor insertion hole (121) where the cover coupling part (121a) is not formed. Accordingly, the cover coupling part (121a) is positioned between the hook (122a) and the upper surface of the sensor cover (122), thereby preventing the sensor cover (122) from coming off.

[0101] The sensor support (122b) may be formed to protrude upward along the circumferential direction from the upper surface of the sensor cover (122). For example, the sensor support (122b) may be formed to protrude in the shape of a circular rib with the radial center of the sensor cover (122) as the origin. The sensor support (122b) may be formed to protrude so as to contact and support the lower surface of the biosensor (400). Thus, when the sensor cover (122) is coupled to the lower housing (120) and blocks the sensor insertion hole (121), the sensor support (122b) can contact and support the biosensor (400). Through this, the biosensor (400) is prevented from shaking, thereby preventing errors from occurring in the measurement value of the biosensor (400).

[0102] Meanwhile, referring to FIGS. 16 and 17, the process of a sensor cover (122) being coupled and fixed to a sensor insertion hole (121) is illustrated. At this time, a cover coupling guide part (123) is disposed in the lower housing (120), and a coupling guide groove (122c) may be formed on the lower surface of the sensor cover (122). The cover coupling guide part (123) is formed so that a user can intuitively recognize whether the coupling state of the sensor cover (122) is fixed or whether the sensor cover (122) is in a detachable state. For example, the cover coupling guide part (123) may be formed on the radial outer side of the sensor insertion hole (121), and an unlock indicator (123a) and a lock indicator (123b) may be disposed at a predetermined interval along the circumferential direction. At this time, a cover coupling part (121a) may be disposed on the radial inner side of the lock indicator (123b). Additionally, the coupling guide groove (122c) may be formed in the shape of a rectangular groove on the lower surface of the sensor cover (122). At this time, a hook (122a) may be positioned along the long axis direction (length direction) of the coupling guide groove (122c). Therefore, the user can separate the sensor cover (122) and the lower housing (120) by directing the longitudinal extension of the coupling guide groove (122c) toward the release indicator (123a). Furthermore, while the sensor cover (122) is blocking the sensor insertion hole (121), the user can lock the coupling state of the sensor cover (122) by rotating the sensor cover (122) so that the longitudinal extension of the coupling guide groove (122c) toward the lock indicator (123b).

[0103] With this configuration, the user can easily attach the sensor cover (122) and intuitively recognize and perform the method of locking or unlocking the attachment state.

[0104] Accordingly, according to the present invention, even when the user's proficiency is low, the biosensor (400) can be easily attached to and detached from the biosensor cartridge (1).

[0105] Meanwhile, a label (130) may be attached to the housing (100). The label (130) may be attached to the upper surface of the upper housing (110). Information about the biosensor cartridge (1) may be displayed on the label (130). For example, the label (130) may display information such as the manufacturer's name, the injection location of the buffer solution, the injection location of the sample solution, and the direction of insertion into the diagnostic device (2).

[0106] Additionally, the label (130) may display a QR code containing sensor information, such as a product ID and a manufacturing serial number, for authenticating the product.

[0107] Meanwhile, FIG. 6 shows an exploded perspective view for explaining a frame according to one embodiment of the present invention, FIG. 7 shows a plan view for explaining a top frame according to one embodiment of the present invention, and FIG. 8 shows a cross-sectional view of a frame according to one embodiment of the present invention.

[0108] Referring to FIGS. 6 to 8, the frame (200) of a biosensor cartridge (1) and the tank (300) formed in the frame according to one embodiment of the present invention are described as follows.

[0109] A frame (200) is positioned inside a housing (100) and can form a channel (600) through which a buffer solution and a sample solution flow. Additionally, a biosensor (400) and a printed circuit board (500) are detachably coupled to the frame (200) so that the biosensor (400) can detect biomaterials in the sample solution flowing through the channel (600).

[0110] The frame (200) includes a base frame (210), a top frame (220), a hydrophilic adhesive layer (230), and a microchannel forming adhesive layer (240). At this time, the hydrophilic adhesive layer (230) may be laminated on the upper side of the base frame (210), the microchannel forming adhesive layer (240) may be laminated on the upper side of the hydrophilic adhesive layer (230), and the top frame (220) may be laminated on the upper side of the microchannel forming adhesive layer (240).

[0111] The base frame (210) can be detachably coupled to the biosensor (400). The base frame (210) can be coupled to the biosensor (400) to support the biosensor (400). For example, the base frame (210) may be formed in the shape of a roughly rectangular flat plate, and a sensor coupling part (211) coupled to the biosensor (400) may be formed.

[0112] The sensor coupling portion (211) may be positioned facing the sensor insertion hole (121). At this time, the sensor coupling portion (211) may be formed to have a diameter smaller than the diameter of the sensor insertion hole (121).

[0113] The sensor coupling portion (211) can be detachably coupled to the biosensor (400). The sensor coupling portion (211) is formed in the shape of a hole corresponding to the shape of the biosensor (400) overall, and a frame may be formed that crosses the hole along the short axis direction. A pair of ports (631, 632) may be formed in the frame.

[0114] Accordingly, the biosensor (400) can be fitted and coupled to the sensor coupling portion (211). Additionally, at least a portion of the side wall surrounding the sensor coupling portion (211) can be formed to be larger than the thickness of the biosensor (400).

[0115] Meanwhile, a hole may be formed in the base frame (210) to fix the valve part (800) and the pump part (900). A fixing member, such as a screw, may pass through the hole and be coupled with the valve part (800) and the pump part (900) positioned on the upper side of the top frame (210).

[0116] A tank (300) may be formed on the top frame (220). Specifically, a tank (300) may be formed on the upper surface of the top frame (220). The tank (300) will be described later.

[0117] A channel (600) may be formed in the top frame (220). Specifically, a channel (600) may be formed on the lower surface of the top frame (220). The channel (600) will be described later.

[0118] At this time, the top frame (220) can be formed from a resin material. For example, the top frame (220) can be formed from PMMA (Polymethyl methacrylate) resin. Through this, it is possible to mass-produce while forming fine channels (600) through injection molding. In addition, there is an advantage that the top frame (220) and the base frame (210) can be easily bonded together using tape.

[0119] A substrate coupling portion (221) may be formed on the upper surface of the top frame (220). A printed circuit board (500) may be detachably coupled to the substrate coupling portion (221). A sensor coupling portion (211) may be disposed on one side of the frame (200), and a substrate coupling portion (221) may be disposed on the other side of the frame (200). At this time, the substrate coupling portion (221) may be disposed in a position facing the sensor coupling portion (211) with the frame (200) in between. Specifically, at least a portion of the substrate coupling portion (221) may be disposed on the upper side of the sensor coupling portion (211).

[0120] The substrate coupling portion (221) may include a coupling guide portion (221a) that guides the slide coupling of the printed circuit board (500). The coupling guide portion (221a) may be formed by protruding a pair facing upward from the upper surface of the top frame (220), and then being bent and extended in a direction facing each other. At this time, the gap between the pair of coupling guide portions (221a) may be equal to the width of the printed circuit board (500). Additionally, the protruding height of the pair of coupling guide portions (221a) may be equal to or slightly larger than the thickness of the printed circuit board (500).

[0121] Additionally, the substrate coupling portion (221) may further include a substrate support portion (221b) that guides the coupling position of the printed circuit board (500) and supports the printed circuit board (500). In this case, the substrate support portion (221b) may be formed to correspond to the shape of both ends in the width direction (short axis direction) of the printed circuit board (500). Additionally, the shortest distance between a pair of substrate support portions (221b) may be formed to be the same as the shortest distance in the width direction of the printed circuit board (500).

[0122] As a result, the substrate coupling portion (221) can prevent the printed circuit board (500) coupled through the coupling guide portion (221a) and the substrate support portion (221b) from shaking in the horizontal and vertical directions.

[0123] Additionally, a clip receiving hole (221c) for receiving a contact clip (450) may be formed in the substrate coupling portion (221). A pair of clip receiving holes (221c) may be arranged on the substrate coupling portion (221). At this time, at least a portion of one of the pair of clip receiving holes (221c) may be placed between a pair of coupling guide portions (221a). Additionally, at least a portion of the other of the pair of clip receiving holes (221c) may be placed between a pair of substrate support portions (221b).

[0124] Additionally, a pair of clip receiving holes (221c) may be formed to communicate with the sensor coupling portion (211). At this time, at least a portion of each of the pair of clip receiving holes (221c) may be positioned facing the sensor coupling portion (211). For example, the sensor coupling portion (211) may be positioned between the pair of clip receiving holes (221c), such that a portion of both ends in the longitudinal direction (front-rear direction) of the sensor coupling portion (211) overlaps with at least a portion of each of the pair of clip receiving holes (221c).

[0125] With this configuration, a base frame (210) and a top frame (220) can be stacked to form a stage to which a contact clip (450) is joined. Additionally, there is an advantage that the contact clip (450) itself can directly contact the biosensor (400) and the printed circuit board (500).

[0126] Meanwhile, a tube receiving portion (222) may be formed in the top frame (220). The tube receiving portion (222) may be formed to protrude upward from the upper surface of the top frame (220). The tube receiving portion (222) may guide the position where the tube (700) is placed on the top frame (220).

[0127] Specifically, the tube receiving portion (222) is formed to protrude in a block shape from the upper surface of the top frame (220), and a pump receiving groove (222a) and a tube guide groove (222b) may be formed inside.

[0128] The pump receiving groove (222a) may accommodate at least a portion of the tube (700) and the pump unit (900) inside. At this time, the tube (700) is arranged along the circumferential direction, and at least a portion of the pump unit (900) may be rotatably accommodated inside the wound tube (700). Additionally, at least a portion of the pump unit (900) may be placed within the pump receiving groove (222a) in contact with the tube (700). For example, the pump receiving groove (222a) may be formed in the shape of a circular groove, the tube (700) may be wound along the inner surface, and the pump unit (900) may be rotatably accommodated inside the tube (700).

[0129] With this configuration, when the pump unit (900) is rotated, the tube (700) placed between the side wall of the pump receiving groove (222a) and the pump unit (900) can be compressed.

[0130] The tube guide groove (222b) is formed to communicate with the pump receiving groove (222a) and can accommodate at least a portion of the tube (700). Specifically, the tube guide groove (222b) can accommodate one side and the other side of the tube (700) wound in the pump receiving groove (222a), respectively. At this time, the tube guide groove (222b) can accommodate the one side and the other side of the tube (700) so that they intersect each other. That is, the groove accommodating one side of the tube (700) and the groove accommodating the other side of the tube (700) can be joined together, and the joined point (222c) can be formed to communicate with the pump receiving groove (222a).

[0131] Through this, when the pump unit (900) rotates, at least a portion of the tube (700) can maintain contact with the pump unit (900). Therefore, backflow in the solution flowing through the tube (700) can be prevented when the pump unit (900) rotates.

[0132] Meanwhile, a valve coupling portion (223) may be formed on the top frame (220). The valve coupling portion (223) may be formed to protrude upward from the upper surface of the top frame (220). For example, the valve coupling portion (223) may be formed to protrude in a rib shape from the upper surface of the top frame (220). The valve coupling portion (223) may be formed to surround the outer side of at least a portion of the valve portion (800). Accordingly, the valve coupling portion (223) can guide the position where the valve portion (800) is coupled.

[0133] Meanwhile, a plurality of ports may be formed in the top frame (220). At this time, the ports may provide a space through which a buffer solution or a sample solution passes and flows into the channel (600), or through which the buffer solution or the sample solution is discharged from the channel (600). Specifically, a buffer solution inlet port (315), a buffer solution port (615), a sample solution port (625), a sensing port (635), a prefill port (645), a waste solution port (655), a first tube connection port (660), and a second tube connection port (670) may be formed in the top frame (220).

[0134] At this time, the buffer solution inlet port (315) is positioned within the buffer solution tank (310) to allow the buffer solution within the buffer solution tank (310) to flow into the channel (600).

[0135] Additionally, the buffer solution port (615), sample solution port (625), sensing port (635), and prefill port (645) may be arranged to be connected by the valve section (800). For example, the buffer solution port (615), sample solution port (625), and prefill port (645) may be arranged on concentric circles with the sensing port (635) as the origin. At this time, the buffer solution port (615), sample solution port (625), and prefill port (645) may be arranged with a predetermined angle difference with the sensing port (635) as the origin.

[0136] Additionally, the first tube connection port (660) and the second tube connection port (670) may be provided to be connected to the tube (700). At this time, the first tube connection port (660) and the second tube connection port (670) may be positioned adjacent to the pump unit (900). For example, the second tube connection port (670) may be positioned on one side in the longitudinal direction of the top frame (220) relative to the pump unit (900), and the first tube connection port (660) may be positioned on the other side in the longitudinal direction of the top frame (220) relative to the pump unit (900).

[0137]

[0138] Meanwhile, in the case of conventional biosensor cartridges, channels were formed inside the frame to allow liquid to flow, but there was a limitation in that the accuracy of sensing was low because the flow rate of the solution was not constant whenever a sample solution was tested.

[0139] To address this, hydrophilic coatings were applied to the base frame; however, while the addition of the coating process increased production costs, it had the disadvantage of raising the defect rate due to coating non-uniformity.

[0140] Accordingly, in the present invention, the flow rate of the solution can be maintained constant through the hydrophilic adhesive layer (230), while minimizing the increase in production costs and the defect rate.

[0141] A hydrophilic adhesive layer (230) may be placed between the base frame (210) and the top frame (220). A hydrophilic adhesive layer (230) may be placed between the base frame (210) and the microchannel forming adhesive layer (240). For example, the hydrophilic adhesive layer (230) may be a hydrophilic tape or a hydrophilic film. The hydrophilic adhesive layer (230) may be placed on the upper side of the base frame (210) to facilitate the flow of fluid flowing through the channel (600). Thus, the hydrophilic adhesive layer (230) has the effect of stably maintaining the flow rate of the solution.

[0142] In addition, there is an advantage that bonding is possible through a simple process of placing a hydrophilic adhesive layer (230), a micro-channel forming adhesive layer (240), and a top frame (220) on the upper side of a base frame (210) and applying pressure, without a separate heating process during the manufacturing process.

[0143] A shape corresponding to the shape of the top frame (220) may be formed in the hydrophilic adhesive layer (230). For example, a clip receiving hole (231) may be formed in the hydrophilic adhesive layer (230). The clip receiving hole (231) of the hydrophilic adhesive layer (230) may be formed in a position facing the clip receiving hole (221c) of the top frame (220) and may be formed in a shape of the same size.

[0144] Additionally, the hydrophilic adhesive layer (230) may be formed with a shape corresponding to the shape of the base frame (210). For example, a port may be formed in the hydrophilic adhesive layer (230). The port of the hydrophilic adhesive layer (230) may be formed in a position facing the port (631a, 632a) of the base frame (210) and may be formed in a shape of the same size.

[0145]

[0146] A microchannel forming adhesive layer (240) can be placed between a hydrophilic adhesive layer (230) and a top frame (220). The microchannel forming adhesive layer (240) can bond the hydrophilic adhesive layer (230) and the top frame (220). At this time, the microchannel forming adhesive layer (240) can be formed with a shape corresponding to the shape of the top frame (220).

[0147] Specifically, the micro-channel forming adhesive layer (240) may have a channel slit (242) formed in communication with the channel (600) formed in the top frame (220). The channel slit (242) may be formed at a position facing the channel (600). At this time, the width of the channel slit (242) may be formed to be larger than the width of the channel (600). With this configuration, even if an error occurs in the position of the channel (600) and the channel slit (242), an error in the flow of the solution can be prevented.

[0148] Additionally, a clip receiving hole (241) may be formed in the micro-channel forming adhesive layer (240). The clip receiving hole (241) of the micro-channel forming adhesive layer (240) may be formed in a position facing the clip receiving hole (221c) of the top frame (220) and may be formed in the same size.

[0149]

[0150] A tank (300) is formed on the upper surface of the frame (200) and can provide a space in which a buffer solution and / or a sample solution can be received. For example, the tank (300) may be formed by protruding from the upper surface of the top frame (220) and forming a shape that surrounds a predetermined space.

[0151] The tank (300) may include a buffer solution tank (310). The buffer solution tank (310) may receive a buffer solution, at least temporarily contain the buffer solution, and allow the buffer solution to flow into a channel (600).

[0152] The buffer solution tank (310) may be positioned on the rear side (one side in the longitudinal direction) of the frame (200). The buffer solution tank (310) may be positioned on the opposite side of the substrate coupling portion (221) on the frame (200).

[0153] Additionally, the buffer solution tank (310) may be placed on the left side (one side in the short direction) of the frame (200). The buffer solution tank (310) may be placed on the opposite side of the waste solution tank (330) on the frame (200).

[0154] The buffer solution tank (310) may be formed to protrude in the form of a wall on the upper side of the top frame (220). At this time, a buffer solution inlet port (315) may be formed in the top frame (220), and the buffer solution inlet port (315) may be formed to communicate with the buffer solution channel (610). The buffer solution inlet port (315) may be formed within the buffer solution tank (310). Additionally, an inclined surface (311) and a guide groove may be formed within the buffer solution tank (310) to guide the buffer solution to flow into the buffer solution inlet port (315).

[0155] The tank (300) may include a sample solution tank (320). The sample solution tank (320) may receive a sample solution, at least temporarily receive the sample solution, and allow the sample solution to flow into a channel (600).

[0156] The sample solution tank (320) may be placed on the rear side of the frame (200). The sample solution tank (320) may be placed on the opposite side of the substrate coupling portion (221) on the frame (200).

[0157] Additionally, at least a portion of the sample solution tank (320) may be positioned in the left-right central part of the frame (200). The sample solution tank (320) may be positioned between the buffer solution tank (310) and the waste solution tank (330).

[0158] The sample solution tank (320) may be formed to protrude in the form of a wall from the upper side of the top frame (220). At this time, the top frame (220) may be formed such that at least a portion of the lower side of the internal space of the sample solution tank (320) is open. The internal space of the sample solution tank (320) may be formed to communicate with the sample solution channel (620).

[0159] The tank (300) may include a waste solution tank (330). The waste solution tank (330) may receive waste solution and store waste solution.

[0160] The waste solution tank (330) may be placed on the rear side of the frame (200). The waste solution tank (330) may be placed on the opposite side of the substrate coupling part (221) on the frame (200).

[0161] Additionally, the waste solution tank (330) can be placed on the right side of the frame (200).

[0162] The waste solution tank (330) may be formed to protrude in the form of a wall on the upper side of the top frame (220). At this time, a waste solution port (655) may be formed in the top frame (220), and the waste solution port (655) may be formed to communicate with the waste solution channel (650). The waste solution port (655) may be formed within the waste solution tank (330).

[0163]

[0164] Meanwhile, FIG. 9 shows a bottom view illustrating a channel formed in a top frame according to one embodiment of the present invention.

[0165] Referring to FIG. 9, the channel (600) of the biosensor cartridge (1) according to one embodiment of the present invention is described as follows.

[0166] A channel (600) is formed inside the frame (200) and can provide a flow path through which a buffer solution or sample solution can be agitated. Specifically, the channel (600) can be formed on the lower side of the top frame (220).

[0167] The channel (600) is connected to the buffer solution tank (310) and includes a buffer solution channel (610) through which the buffer solution flows. Specifically, one side of the buffer solution channel (610) may be in communication with a buffer solution inlet port (315). Additionally, the other side of the buffer solution channel (610) may be in communication with a buffer solution port (615). The other side of the buffer solution channel (610) may be in communication with a sensing channel (630) through the buffer solution port (615) depending on the operation of the valve section (800).

[0168] The buffer solution channel (610) can guide the buffer solution flowing into the buffer solution tank (310) to the valve section (800).

[0169] Meanwhile, the buffer solution inlet port (315) can serve as an inlet for the buffer solution channel (610), and the buffer solution port (615) can serve as an outlet for the buffer solution channel (610).

[0170] At this time, the buffer solution channel (610) may be formed with a width on the inlet side and a width on the outlet side that are different. For example, the buffer solution channel (610) may be formed with a width on the outlet side that is larger than the width on the inlet side. Through this, the buffer solution can be sufficiently present on the outlet side of the buffer solution channel (610), and the flow rate of the buffer solution passing through the valve section (800) can be prevented from decreasing. Therefore, there is an effect of maintaining a stable flow rate of the buffer solution.

[0171] The channel (600) includes a sample solution channel (620) through which the sample solution flows, which is connected to the sample solution tank (320). Specifically, one side of the sample solution channel (620) may be in communication with the internal space of the sample solution tank (320). Additionally, the other side of the sample solution channel (620) may be in communication with the sample solution port (625). The other side of the sample solution channel (620) may be in communication with the sensing channel (630) through the sample solution port (625) according to the operation of the valve section (800).

[0172] The sample solution channel (620) can guide the sample solution flowing into the sample solution tank (320) to the valve section (800). Accordingly, the internal space of the sample solution tank (320) can serve as the inlet of the sample solution channel (620), and the sample solution port (625) can serve as the outlet of the sample solution channel (620).

[0173] Meanwhile, the internal space of the sample solution tank (320) can serve as an inlet for the sample solution channel (620), and the sample solution port (625) can serve as an outlet for the sample solution channel (620).

[0174] At this time, the diameter of the internal space of the sample solution tank (320) may be formed to be larger than the width of the discharge side of the sample solution channel (620). Additionally, the width of at least a portion of the sample solution channel (620) may be changed. For example, the sample solution channel (620) may be formed such that the width of the inlet side and the width of the discharge side are the same, while having a section where the width narrows in between.

[0175] Through this, it is possible to prevent the flow rate of the sample solution flowing through the sample solution channel (620) from decreasing instantaneously depending on the operation of the pump unit (900). Therefore, there is an effect of maintaining the flow rate of the sample solution stably.

[0176] The channel (600) may include a sensing channel (630) that is connected to a buffer solution channel (610) or a sample solution channel (620) and guides the buffer solution or sample solution to the biosensor (400). Specifically, one side of the sensing channel (630) may be in communication with a sensing port (635). Additionally, the other side of the sensing channel (630) may be in communication with a first tube connection port (660). The other side of the sensing channel (630) may be in communication with a flow path formed inside the tube (700) through the first tube connection port (660) according to the operation of the valve part (800).

[0177] The sensing channel (630) can guide the buffer solution or sample solution introduced through the valve section (800) to the biosensor (400) and allow it to pass through the biosensor (400). Specifically, the sensing channel (630) may include a first sensing channel (631) and a second sensing channel (632).

[0178] At this time, the first sensing channel (631) can guide the buffer solution or sample solution that has passed through the valve section (800) to the biosensor (400). Specifically, the first sensing channel (631) can flow the buffer solution or sample solution into the internal space of the sensor coupling section (211) through the inlet port (631a) formed in the base frame (210).

[0179] With this configuration, the buffer solution or sample solution that has passed through the first sensing channel (631) can flow on the upper surface of the biosensor (400).

[0180] Meanwhile, the sensing port (635) can serve as an inlet for the first sensing channel (631), and the sensor coupling part (211) can serve as an outlet for the first sensing channel (631).

[0181] At this time, the width of the inlet side of the first sensing channel (631) can be formed to be larger than the width of the outlet side of the first sensing channel (631). Through this, the buffer solution or sample solution can be sufficiently present on the inlet side of the first sensing channel (631), and the flow rate of the buffer solution or sample solution flowing through the first sensing channel (631) instantaneously according to the operation of the pump unit (900) can be prevented from decreasing. Therefore, there is an effect that the flow rate of the buffer solution or sample solution can be stably maintained.

[0182] Additionally, the second sensing channel (632) can guide the buffer solution or sample solution that has passed through the biosensor (400) into the tube (700). At this time, the second sensing channel (632) can be positioned in a straight line downstream of the first sensing channel (631). With this configuration, the flow path passing through the biosensor (400) can be formed in a straight line, and the flow rate and / or flow rate of the buffer solution or sample solution flowing through the biosensor (400) can be stably maintained.

[0183] The channel (600) may include a prefill channel (640) through which the sample solution flows, which is in communication with the sample solution channel (620). Specifically, one side of the prefill channel (640) may be in communication with the sample solution channel (620). Additionally, one side of the prefill channel (640) may be in communication with the sample solution port (625). Additionally, the other side of the prefill channel (640) may be in communication with the prefill port (645).

[0184] The prefill channel (640) can guide the sample solution that has passed through the sample solution channel (620) to the prefill port (645). For example, the prefill channel (640) is formed to communicate with the sample solution channel (620) and can be formed by bending it multiple times to communicate with the prefill port (645).

[0185] At this time, the width of at least a portion of the prefill channel (640) can be changed.

[0186] Additionally, the prefill channel (640) may be equipped with a valve (641) formed to be wider than the inlet and outlet. The valve (641) may be formed to be the widest in the prefill channel (640), while the width of the inlet entering the valve (641) may be formed to be the narrowest in the prefill channel (640). Through this, when a large amount of sample solution is instantaneously introduced into the prefill channel (640), the sample solution may be prevented from being discharged into the prefill port (645).

[0187] The channel (600) may include a waste solution channel (650) that guides the buffer solution or sample solution passing through the tube (700) to the waste solution tank (330). Specifically, one side of the waste solution channel (650) may be in communication with a second tube connection port (670). Additionally, the other side of the waste solution channel (650) may be in communication with a waste solution port (655). Thus, when the pump unit (900) is operated, the buffer solution or sample solution passing through the tube (700) can be stored in the waste solution tank (330).

[0188]

[0189] Meanwhile, FIG. 10 illustrates a diagram for explaining the coupling of a biosensor and a printed circuit board to a frame in a biosensor cartridge according to an embodiment of the present invention, FIG. 11 illustrates a diagram for explaining a sensor coupling part in a biosensor cartridge according to an embodiment of the present invention, FIG. 12 illustrates a diagram for explaining the state in which a biosensor is coupled in a biosensor cartridge according to an embodiment of the present invention, FIG. 13 illustrates a cross-sectional view for explaining the contact between a biosensor and a printed circuit board in a biosensor cartridge according to an embodiment of the present invention, FIG. 14 and FIG. 15 illustrate cross-sectional views for explaining the arrangement of a biosensor and a printed circuit board and the arrangement of a flow path flowing over the biosensor in a biosensor cartridge according to an embodiment of the present invention.

[0190] Referring to FIGS. 10 to 15, the biosensor (400), contact clip (450), and printed circuit board (500) of the biosensor cartridge (1) according to one embodiment of the present invention are described as follows.

[0191] The biosensor (400) is detachably coupled to the frame (200) and can detect biomaterials. The biosensor (400) can be inserted through the sensor insertion hole (121) of the lower housing (120), and the biosensor (400) can be coupled to the sensor coupling part (211) of the base frame (210).

[0192] A biosensor (400) can selectively detect a minute amount of biomaterial to be analyzed by combining a bioreceptor having a recognition function for a specific biomaterial with an electrical transducer to convert biological interactions and recognition responses into electrical signals.

[0193] The biosensor (400) of the present invention may be an electrochemical-based biosensor. An electrochemical-based biosensor combines the analytical capability of an electrochemical method with the specificity of biological recognition, and detects the biological recognition phenomenon of a target substance as a change in current or potential by immobilizing or containing a substance having biological specificity, such as an enzyme, antigen, antibody, or biochemical substance, on the electrode surface.

[0194] For example, a sensing portion may be provided on the upper surface of the biosensor (400). A bio-receptor may be placed in the sensing portion. A buffer solution and a sample solution may flow through the sensing portion. The sensing portion may be connected to a circuit to transmit an electrical signal generated in the sensing portion.

[0195] A circuit may be placed on the biosensor (400) and may be electrically connected to the printed circuit board (500) by a contact clip (450).

[0196] A biosensor (400) according to one embodiment of the present invention comprises a graphene-based field effect transistor (FET) using a graphene layer as a channel.

[0197] Meanwhile, when a sample solution is introduced and a corresponding voltage is applied to the source electrode, drain electrode, and gate electrode, respectively, if a target substance is present in the sample solution, the graphene layer becomes charged with a specific carrier as the target substance reacts with the sensing substance. Accordingly, a depletion state in which charge accumulates in the graphene layer proceeds, and the drain current flowing through the drain electrode increases.

[0198] Meanwhile, when a sample solution is introduced and the corresponding voltage is applied to the source electrode, drain electrode, and gate electrode, respectively, if there is no target substance in the sample solution, the drain current flowing through the drain electrode flows at a significantly lower level than the drain current when the target substance is present.

[0199] Meanwhile, the sample solution may refer to a solution diluted with biological material such as saliva, body fluids including sweat, blood, serum, or plasma.

[0200]

[0201] A sealer (410) may be disposed on the upper side of the biosensor (400). The sealer (410) may be disposed on the sensor coupling portion (211). A flow path forming portion (411) may be formed in the sealer (410). For example, the sealer (410) may be formed in the shape of a rectangular parallelepiped, and the flow path forming portion (411) may be in the shape of a slit formed along the left and right directions.

[0202] At this time, the flow path forming part (411) may be positioned on the lower side of the frame formed in the sensor coupling part (211). Specifically, the flow path forming part (411) may be positioned on the lower side of the inlet port (631a) and the outlet port (632a) formed in the sensor coupling part (211), and may be in communication with the inlet port (631a) and the outlet port (632a).

[0203] Additionally, the flow path forming part (411) may be positioned above the sensing part of the biosensor (400). At this time, the sealer (410) may be formed to surround the outer edge of the sensing part. The sealer (410) seals the outer edge of the flow path forming part (411) to prevent the buffer solution and sample solution flowing inside the flow path forming part (411) from leaking out.

[0204] The printed circuit board (500) can be detachably coupled to the frame (200). At least a portion of the biosensor (400) can be inserted into the upper housing (110) and detachably coupled to the substrate coupling portion (221) of the top frame (220). The printed circuit board (500) can be supported by the coupling guide portion (221a) and the substrate support portion (221b).

[0205] Specifically, the printed circuit board (500) may include a board body (510), a connector (520), and a guide portion (530). For example, the board body (510) may be formed in the shape of a roughly rectangular flat plate. A circuit may be mounted on the board body (510) and electrically connected to the biosensor (400).

[0206] Meanwhile, a connector (520) may be provided at the front end of the substrate body (510). The connector (520) may be connected to a circuit provided in the substrate body (510). Thus, when the biosensor cartridge (1) is coupled to the diagnostic device (2), an electrical signal can be transmitted to the diagnostic device (2) through the connector (520). Additionally, when the biosensor cartridge (1) is coupled to the diagnostic device (2), power can be applied to the printed circuit board (500) and the biosensor (400) through the connector (520).

[0207] The guide portion (530) can be formed at both ends of the left and right directions of the substrate body (510) and can be coupled with the substrate support portion (221b) of the substrate coupling portion (221).

[0208] Meanwhile, a contact clip (450) may be disposed between the biosensor (400) and the printed circuit board (500). The contact clip (450) is coupled to the sensor coupling portion (211) and may come into contact with the printed circuit board (500) and the biosensor (400). Additionally, the contact clip (450) may be disposed to pass through the clip receiving hole (221c) and to pass through the hole of the sensor coupling portion (211), while at least a portion may be supported by the base frame (210).

[0209] Multiple contact clips (450) may be provided. An even number of contact clips (450) may be provided. In this case, multiple pairs of contact clips (450) may be arranged side by side, while one pair may be symmetrically arranged in a position facing each other.

[0210] Specifically, the contact clip (450) may be formed of a conductive material. For example, the contact clip (450) may be formed of a metal material. Each contact clip (450) may include a substrate contact portion (451), a sensor contact portion (452), and a connection portion (453).

[0211] The substrate contact portion (451) can be positioned to pass through the clip receiving hole (221c). At this time, the substrate contact portion (451) can be in contact with a terminal (not shown) provided on the printed circuit board (500). That is, the substrate contact portion (451) can be electrically connected to a circuit mounted on the printed circuit board (500).

[0212] The sensor contact portion (452) is positioned to pass through a hole formed in the sensor coupling portion (211) and can come into contact with a terminal (not shown) provided in the biosensor (400). That is, the sensor contact portion (452) can be electrically connected to a circuit mounted on the biosensor (400).

[0213] At this time, the total height of the contact clip (450) in the vertical direction can be formed to be longer than the shortest distance between the biosensor (400) and the printed circuit board (500).

[0214] With this configuration, when the sensor contact portion (452) comes into contact with the biosensor (400), the sensor contact portion (452) can apply pressure to the biosensor (400) while elastically deforming, and can maintain a strong contact state while minimizing the contact area with the biosensor (400).

[0215] The connecting portion (453) may be formed to connect the substrate contact portion (451) and the sensor contact portion (452). At this time, the connecting portion (453) is formed by bending and extending downward from the substrate contact portion (451), and then is bent and extended along the front-rear direction, and can be connected to the sensor contact portion (452) by bending and extending downward. The connecting portion (453) may be supported by contacting the base frame (210).

[0216] Therefore, the contact clip (450) is seated on the frame (200), so that the upper side is in contact with the printed circuit board and the lower side is in contact with the biosensor (400).

[0217]

[0218] Meanwhile, FIG. 18 shows an exploded perspective view for explaining the valve section and the pump section in a biosensor cartridge according to one embodiment of the present invention, FIG. 19 shows an exploded perspective view for explaining the valve section in a biosensor cartridge according to one embodiment of the present invention, FIG. 20 shows a perspective view for explaining the channel plate of the valve section in a biosensor cartridge according to one embodiment of the present invention, FIG. 21 shows a bottom view for explaining the pump section in a biosensor cartridge according to one embodiment of the present invention, and FIG. 22 shows a diagram for explaining the process of compressing a tube according to the rotation of the pump section in a biosensor cartridge according to one embodiment of the present invention.

[0219] Referring to FIGS. 18 to 22, the tube (700), valve part (800), and pump part (900) of a biosensor cartridge (1) according to one embodiment of the present invention are described as follows.

[0220] The tube (700) is connected to the channel (600) and can flow a buffer solution or a sample solution. The tube (700) is formed of a flexible material and has a flow path formed inside through which fluid can flow.

[0221] One end of the tube (700) can be connected to a first tube connection port (660), and the other end of the tube (700) can be connected to a second tube connection port (670). Through this, one side of the tube (700) can be connected to a sensing channel (630), and the other side of the tube (700) can be connected to a waste solution channel (650). Accordingly, the tube (700) can receive a buffer solution or sample solution that has passed through the sensing channel (630) and discharge the buffer solution or sample solution to the waste solution channel (650).

[0222] The tube (700) can be coupled to the tube receiving portion (222). Specifically, the tube (700) can be positioned along the inner circumference of the pump receiving groove (221a) to wrap around at least a portion of the pump knob (910). At this time, a portion of the tube (700) can be elastically deformed by being pressed between the pump knob (910) and the inner circumference of the pump receiving groove (221a). Thus, when the pump knob (910) is rotated, the tube (700) is sequentially pressed along the circumference, causing the buffer solution or sample solution inside to flow. Additionally, as the buffer solution or sample solution within the tube (700) flows, negative pressure is formed in the sensing channel (630), causing the buffer solution or sample solution to flow in.

[0223] Meanwhile, in a conventional structure in which fluid flows by pressing the tube according to the rotation of the pump, even if the inlet side and the discharge side are positioned close together, the tube bends sharply in the region just before the tube is wound in a circle, and since the inlet side and the discharge side bend in opposite directions, a region is created in which the tube cannot be pressed while the pump rotates 360 degrees.

[0224] In this case, as the tube compressed by the pump is instantaneously restored, backflow occurs in the fluid flowing through the tube. Consequently, there is a problem of backflow occurring in the entire fluid path. In particular, when the flow rate of a buffer solution or sample solution must be maintained stably, such as in a biosensor cartridge, there is a problem that errors may occur in the measurement values.

[0225] To solve this, in the present invention, the tube (700) is arranged along the circumferential direction so as to surround the pump knob (910), with the inlet side and the discharge side intersecting each other. In this case, either the inlet side or the discharge side of the tube (700) may be stacked on top of the other.

[0226] Specifically, one side and the other side in the longitudinal direction of the tube (700) can be received in the tube guide groove (222b), respectively. At this time, the tube guide groove (222b) can be formed so that the groove receiving one side of the tube (700) and the groove receiving the other side of the tube (700) merge into one and communicate with the pump receiving groove (222a). Then, the one side and the other side of the tube (700) can be received in a stacked state in the tube guide groove (222b) that has merged into one.

[0227]

[0228] The valve part (800) can be rotatably coupled to the frame (200).

[0229] Specifically, the valve section (800) includes a valve housing (810), a valve knob (820), and a channel plate (830). At this time, at least a portion of the valve knob (820) is rotatably accommodated inside the valve housing (810), and the channel plate (830) can be coupled to the lower side of the valve knob (820).

[0230] The valve portion (800) can be coupled to the upper side of the top frame (220). Specifically, the valve housing (810) can be coupled to the valve coupling portion (223). At this time, the valve housing (810) can be fixed to the frame (200) by being coupled with a fixing member such as a screw. At this time, the fixing member can be coupled to the valve housing (810) by penetrating the base frame (210) and the top frame (220).

[0231] For example, the valve housing (810) may include a fixed part (811) coupled to the upper side of the top frame (220) and a support part (812) extending upward from the fixed part. On the other hand, the support part (812) may be formed in a cylindrical shape, and the inner diameter of the support part (812) may be formed smaller than the inner diameter of the knob receiving hole of the fixed part (811). That is, the inner surface of the valve housing (810) may be formed to form a step.

[0232] The valve knob (820) may be provided to rotate when an external force is applied.

[0233] At least a portion of the valve knob (820) may be rotatably received in the valve housing (810). The valve knob (820) comprises a large diameter portion (821) and a small diameter portion (822), wherein the outer diameter of the large diameter portion (821) is formed to be larger than the outer diameter of the small diameter portion (822), and the large diameter portion (821) may be positioned lower than the small diameter portion (822). Additionally, the large diameter portion (821) may be received in the fixed portion (811) of the valve housing, and the small diameter portion (822) may be received in the support portion (812) of the valve housing.

[0234] By doing so, the valve knob (820) can be prevented from moving along the axial direction.

[0235] Meanwhile, a knob groove (823) may be formed in the valve knob (820) so that an external force can be applied. The knob groove (823) may be coupled to a valve actuator provided in the diagnostic device (2) when the biosensor cartridge (1) is coupled to the diagnostic device (2). At this time, the knob groove (823) may be formed to guide the initial position when the valve knob (820) is rotated. For example, the knob groove (823) may be formed with an uneven diameter. Accordingly, the knob groove (823) may be key-coupled to the valve actuator. With this configuration, the knob groove (823) and the valve actuator can be coupled in the correct position.

[0236] Meanwhile, the channel plate (830) is coupled to the lower side of the valve knob (820) and can rotate together with the rotation of the valve knob (820). Specifically, the channel plate (830) may include a plate body (831) and a coupling guide hole (832). For example, the plate body (831) may be formed in the shape of a disc and coupled to the lower end of the valve knob (820). At this time, at least one coupling projection (not shown) may be formed protruding from the lower surface of the valve knob (820). Along with this, a coupling guide hole (832) may be formed in the plate body (831) at a position facing the coupling projection.

[0237] With this configuration, when the coupling projection of the valve knob (820) and the coupling guide hole (832) are coupled, the valve knob (820) and the channel plate (830) can rotate in conjunction. Additionally, there is an advantage in that the position of the connecting channel (833), which will be described later, can be determined through the degree to which the knob groove (823) is rotated.

[0238] A connecting channel (833) may be formed in the channel plate (830). The connecting channel (833) is formed on the lower surface of the channel plate (830) and can connect at least two ports formed in the top frame (220) to each other according to the rotation of the channel plate (830).

[0239] The connection channel (833) can be formed to connect at least two of the buffer solution port (615), sample solution port (625), sensing port (635) and prefill port (645) to each other.

[0240] Accordingly, the sensing port (635) can be connected to any one of the buffer solution port (615), sample solution port (625), and prefill port (645) by the connection channel (833), and when the pump unit (900) is operated, the buffer solution or sample solution can be introduced into the sensing port (635).

[0241] Therefore, the valve knob (820) can open and close the channel (600) to selectively flow the buffer solution or sample solution into the biosensor (400) depending on the rotation.

[0242]

[0243] The pump unit (900) is rotatably coupled to the frame (200) and, as it rotates, pressurizes the tube (700) to generate a flow force in the buffer solution or sample solution flowing through the channel (600).

[0244] The pump unit (900) may include a pump knob (910) and a bearing (920).

[0245] The pump knob (910) can be positioned on the upper surface of the top frame (220). The pump knob (910) can be rotatably coupled within the tube receiving portion (222). The pump knob (910) can be rotatably received within the pump receiving groove (222a).

[0246] Specifically, the pump knob (910) includes a shaft (911), a support (912), and a drive groove (913). The shaft (911) is formed in a cylindrical shape and provides the rotation axis of the pump knob (910). A cam (911a) may be formed on the shaft (911) such that the radius from the rotation axis is the largest. Thus, since the shaft (911) has an outer surface in the shape of a cam, the position where the tube (700) is pressed can be changed according to the rotation of the shaft (911).

[0247] The support member (912) is positioned on the upper part of the shaft (911) and can be positioned on the lower side of the upper housing (110).

[0248] The support member (912) can be formed in the shape of a circular block. The support member (912) can be formed integrally with the shaft (911). Therefore, the support member (912) and the shaft (911) can rotate together.

[0249] The outer diameter of the support member (912) may be formed to be larger than the maximum outer diameter of the shaft (911). At this time, the diameter of the support member (912) may be larger than the diameter of the combined state of the shaft (911), the bearing (920) surrounding the outer surface of the shaft, and the tube (700) surrounding the outer surface of the bearing (920). For example, the diameter of the support member (912) may be equal to the diameter of the pump receiving groove (222a).

[0250] The driving groove (913) may be positioned on the top of the support member (912). The driving groove (913) may be formed to allow external force to be applied. When the biosensor cartridge (1) is coupled to the diagnostic device (2), the driving groove (913) may be coupled to a pump actuator provided in the diagnostic device (2). The driving groove (913) may be formed corresponding to the shape of the pump actuator. For example, the driving groove (913) may be formed in the shape of a cross groove.

[0251] Therefore, after the pump actuator is coupled to the drive groove (913), when the pump actuator rotates, rotational force can be applied to the pump knob (910).

[0252] The bearing (920) can wrap around the outer surface of the shaft (911). The bearing (920) can come into contact with the tube (700) when the shaft (911) rotates. This prevents the shaft (911) from directly rubbing against the tube (700).

[0253]

[0254] Although the present invention has been described in detail through specific embodiments, this is for the purpose of specifically explaining the invention and is not limited thereto. It is evident that modifications or improvements to the present invention are possible by those skilled in the art within the technical scope of the invention.

[0255] All simple variations or modifications of the present invention fall within the scope of the present invention, and the specific scope of protection of the present invention will be clarified by the appended claims.

Claims

1. Housing; A frame disposed within the above housing; A biosensor detachably coupled to the above frame; A channel formed in the above frame and providing a path for the buffer solution or the sample solution to flow through; Includes, In the above housing, A sensor input hole is formed through which the above biosensor can pass, and The sensor input hole above is, A biosensor cartridge characterized by a sensor cover that is detachably coupled.

2. In Paragraph 1, The above frame is, A sensor coupling part to which the above biosensor is detachably coupled; A biosensor cartridge containing 3. In Paragraph 2, The diameter of the sensor input hole above is, A biosensor cartridge characterized by having a diameter larger than that of the sensor coupling portion.

4. In Paragraph 1, A biosensor cartridge characterized by having a pair of cover coupling portions formed protruding radially inward on at least a portion of the side wall surrounding the sensor insertion hole.

5. In Paragraph 4, The sensor cover above is, A hook formed protruding from the upper surface of the sensor cover and formed along the circumferential direction for a predetermined angle range; A biosensor cartridge containing 6. In Paragraph 5, The above hook is, A biosensor cartridge characterized by being coupled to the cover coupling portion according to the rotation of the sensor cover.

7. In Paragraph 1, The sensor cover above is, A sensor support member protruding from the upper surface of the sensor cover; Includes more, A biosensor cartridge characterized in that when the sensor cover is coupled to the housing and blocks the sensor insertion hole, the sensor support contacts and supports the biosensor.

8. In Paragraph 2, A sealer disposed between the biosensor and the sensor coupling portion, having a fluid path forming portion communicating with the channel; A biosensor cartridge containing 9. In Paragraph 2, A contact clip coupled to the sensor coupling portion and in contact with the biosensor and the printed circuit board; A biosensor cartridge containing additional 10. Housing; A frame disposed within the above housing; A biosensor detachably coupled to one side of the above frame and detecting biomaterials; A printed circuit board detachably coupled to the other side of the above frame; A channel formed in the above frame and providing a path for the buffer solution or the sample solution to flow through; A sealer disposed between the above frame and the above biosensor; A contact clip that penetrates the frame and contacts the biosensor and the printed circuit board; Includes, The above frame is, A sensor coupling part to which the above biosensor is detachably coupled; Includes, In the above housing, A biosensor cartridge characterized by having a sensor insertion hole formed so as to allow the biosensor to pass through, and formed at a position facing the sensor coupling part.

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