Basic turmeric extract and preparation method therefor

Abstract

The present invention provides a method for preparing a basic turmeric extract, the method comprising the steps of: (a) adding water into a water tank having an air circulation passage provided at an upper portion thereof and having, at a lower bottom thereof, a magnet having a magnetic flux density of 10,000 Gauss or more and a silicon-based mineral plate containing mica and pegmatite, and then adding and mixing mica powder and seafood shell powder (including internal sand of seafood shells), followed by stirring the mixed solution for 16-72 hours; (b) transferring the solution obtained in step (a) into a hot-water device maintained at 48°C or less and having an air circulation passage provided at an upper portion thereof, and then stirring the solution for 12-36 hours while applying electrical energy through AC of a voltage of 220 V and a frequency of 60 Hz; (c) sealing the air circulation passage provided at the upper portion of the hot-water device to be in a vacuum state, and then dissolving 0.85-0.95% of salt in the solution obtained in step (b), and stirring the solution at atmospheric pressure and a temperature of 48°C or less for 15-24 hours, to thereby obtain a basic turmeric extract; (d) adding turmeric powder, salt, and seafood shell powder to the solution obtained in step (c) and mixing same, followed by stirring the mixed solution at atmospheric pressure and a temperature of 48°C or less for 1-3 hours, to thereby obtain a basic turmeric extract; (e) aging the basic turmeric extract obtained in step (d) at room temperature for 15-18 days; and (f) maintaining the aged basic turmeric extract obtained in step (e) in a vacuum state for 9-11 hours to thereby obtain a basic turmeric extract. Also, the present invention provides a basic turmeric extract prepared by the preparation method.

Description

Basic turmeric extract and method for preparing the same

[0001] The present invention relates to a basic turmeric extract and a method for preparing the same.

[0002] Turmeric (Curcuma linga L.) is a perennial herb belonging to the ginger family, distributed in tropical and subtropical regions, and native to India and Southeast Asia. Turmeric has long been used to treat inflammation, and it is known for its excellent anticancer effects, which inhibit the growth of proteins produced due to the efficacy of its similar chemical structural components, led by curcumin, a bioactive substance reported to be present in large quantities. Furthermore, research on curcumin reveals various findings regarding its physiological activities in the human body, including antioxidant, anticancer, anti-inflammatory, and antiviral properties.

[0003] Curcumin [1,7-bis-(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione], the main component of turmeric, is the substance that gives turmeric its yellow color, and many research results have been published as it has been revealed to have anti-inflammatory, antioxidant, and anticancer effects.

[0004] Curcumin exists in nature in the form of diferuloylmethane, demethoxycurcumin, and bis-demethoxycurcumin. Among these, diferuloylmethane is the most stable and has high bioavailability, but it has the disadvantage of low absorption rate because it is a fat-soluble component and does not dissolve well in water.

[0005] Curcumin is yellow in acidic conditions and red in basic conditions. Curcumin is a keto-enol tautomeric compound that exists in the enol form in organic solvents and in the keto form in water.

[0006]

[0007] The expected biosynthetic pathway of curcumin is as follows, but it has not yet been clearly identified.

[0008]

[0009] The predicted pathways for curcumin biosynthesis proposed so far are broadly divided into two types. The first pathway involves arylation into curcuminoid through a chain-extending reaction carried out by cinnamic acid and five malonyl-CoA molecules, and the second pathway involves the process in which two cinnamic acids are combined by malonyl-CoA.

[0010] In reality, curcumin has many limiting factors in the analysis and evaluation of its pharmacological effects, such as a chemically unstable structure, poor solubility in water, low bioavailability, and limited tissue distribution.

[0011] Conventionally, turmeric extracts were prepared by a hot water extraction method at 100–180°C under ultra-high pressure, which resulted in high production costs, acidic conditions during storage, and limitations in the analysis and evaluation of pharmacological effects.

[0012] After extensive research, the inventors devised a method for producing a basic turmeric extract by applying electric and magnetic energy at atmospheric pressure and a temperature of 48°C or lower, thereby enabling the mass production of a basic turmeric extract that is stable even during long-term storage while significantly reducing production costs.

[0013] The objective of the present invention is to provide a method for manufacturing a basic turmeric extract that can be mass-produced while significantly reducing production costs and maintaining stability even during long-term storage.

[0014] In addition, another objective of the present invention is to provide a basic turmeric extract prepared by the above-described manufacturing method, comprising diferuloylmethane, demethoxycurcumin, and bis-demethoxycurcumin, having a pH of 10 to 12, and being stable even during long-term storage.

[0015] The present invention comprises the steps of: (a) introducing water into a water tank having an air circulation passage at the top and a silicon-based mineral plate containing mica and coarse-grained stone arranged on the bottom, with a magnet having a magnetic flux density (magnetic flux density) of 10,000 Gauss or more; adding and mixing mica powder and shellfish shell powder (including internal sand of shellfish shells); and stirring the mixed solution for 16 to 72 hours; (b) transferring the solution produced in step (a) into the interior of a water heater at 48°C or lower having an air circulation passage at the top, and stirring the solution for 12 to 36 hours while applying electrical energy through alternating current of 220V and 60Hz frequency; (c) sealing an air circulation passage provided at the top of the hot water device to create a vacuum, then dissolving salt at a concentration of 0.85 to 0.95% in the solution produced in step (b), and stirring for 15 to 24 hours at atmospheric pressure and a temperature of 48°C or lower; (d) adding turmeric powder, salt, and shellfish shell powder to the solution produced in step (c), mixing them, and then stirring the mixed solution for 1 to 3 hours at atmospheric pressure and a temperature of 48°C or lower to obtain a basic turmeric extract; (e) aging the basic turmeric extract produced in step (d) at room temperature for 15 to 18 days; and (f) maintaining the basic turmeric extract aged in step (e) under a vacuum for 9 to 11 hours to provide a method for producing a basic turmeric extract.

[0016] In the method for preparing a basic turmeric extract of the present invention, in step (a), the weight ratio of mica powder to fish shell powder is preferably 7:3.

[0017] In the method for preparing a basic turmeric extract of the present invention, the shell powder is preferably the shell powder of a shellfish selected from the group consisting of coral reefs, oysters, and scallops.

[0018] In the method for preparing a basic turmeric extract of the present invention, the mixed solution in step (d) is composed of 92-94% (w / v) of solution, 3-5% (w / v) of turmeric powder, 1.25-1.78% (w / v) of salt, and 0.25-0.35% (w / v) of fish and shellfish shell powder.

[0019] In the method for preparing a basic turmeric extract of the present invention, the pH of the basic turmeric extract is 10 to 12.

[0020] In addition, the present invention provides a basic turmeric extract prepared by the above-described manufacturing method, comprising diferuloylmethane, demethoxycurcumin, and bis-demethoxycurcumin, and having a pH of 10 to 12.

[0021] The basic turmeric extract of the present invention has a curcumin content of 0.28 mg / L.

[0022] The basic turmeric extract of the present invention has 9561 mg / L of Cl. - , 13.9 mg / L of NO3 - , 334.9 mg / L of SO4 2- It contains 5684 mg / L of Na, 817 mg / L of Ca, 41 mg / L of Mg, 1097 mg / L of K, and 8.1 mg / L of Si.

[0023] According to the present invention, there is an advantage in being able to mass-produce a basic turmeric extract that remains stable even during long-term storage while significantly reducing production costs.

[0024] Figure 1 shows the high-performance liquid chromatography (HPLC) results of curcumin, fine panax (solution) + turmeric powder, and basic turmeric extract. The three main peaks of the basic turmeric extract represent, in order, bis-demethoxycurcumin (bdmc), demethoxycurcumin (dmc), and curcumin (ccm) [diferuloylmethane].

[0025] Figure 2 shows the emissivity of a basic turmeric extract according to the present invention.

[0026] Figure 3 shows the radiation energy of a basic turmeric extract according to the present invention.

[0027] The present invention will be described in detail by the following examples. The following examples are illustrative of preferred embodiments of the present invention, and therefore should not be interpreted as limiting the scope of the present invention.

[0028] Example 1: Preparation of Basic Turmeric Extract

[0029] Water was poured into a tank equipped with an air circulation passage at the top and a silicon-based mineral plate containing mica and coarse-grained stone, along with a magnet having a magnetic flux density of 10,000 Gauss or more, placed on the bottom. Then, mica powder and fish shell powder (including the internal sand of the fish shells) were added and mixed, and the mixed solution was stirred for about 72 hours. Subsequently, the resulting solution was transferred into a 48°C water heater equipped with an air circulation passage at the top, and the solution was stirred for about 36 hours while applying electrical energy through alternating current of 220V and 60Hz frequency. Subsequently, the air circulation passage at the top of the water heater was sealed to create a vacuum, salt with a concentration of 0.85–0.95% was dissolved, and the solution was stirred for about 20 hours at atmospheric pressure and a temperature of 48°C. The resulting solution is referred to as "Fine Panax (solution)." Next, 4% (w / v) of turmeric powder, 1.75% (w / v) of salt, and 0.35% (w / v) of fish and shellfish shell powder were added to 93.9% (w / v) of Fine Panax (solution) and mixed. Then, the mixed solution was stirred for 2 hours at atmospheric pressure and a temperature of 48°C to produce a basic turmeric extract. Subsequently, the produced basic turmeric extract was aged at room temperature for about 16 days. Subsequently, the aged basic turmeric extract was maintained under vacuum for about 10 hours to obtain the final basic turmeric extract.

[0030] Example 2: Component analysis of Fine Panax (solution) and Turmeric extract

[0031] The results of the component analysis of Fine Panax (solution) and basic turmeric extract obtained by commissioning the Korea Quality Testing Institute (KQT) are presented in Table 1 below.

[0032] Ingredient Content (mg / L) Fine Panax Turmeric Extract Cl - 107.59561NO3 - 8.413.9SO42- 21.7334.9Na335684Ca137817Mg0.341K2.51097Si4.48.1

[0033] Example 3: High-performance liquid chromatography (HPLC) analysis of basic turmeric extract. High-performance liquid chromatography (HPLC) analysis was performed by the Korea Basic Science Institute Seoul Center. The analysis conditions are presented in Table 2 below.

[0034] Column: Kinetex C18 (46 X 250 mm, 5 µm, Phenomenex) Guard Column: UHPLC C18 (AJ0-8768, Phenomenex) Column Temperature: 35 ℃ Mobile Phase: A: 0.1% Trifluoroacetic Acid (TFA) B: 0.1% TFA / Acetonitrile Gradient * Equilibrium: 5 mins, Flow rate 1.0 mL / wavelength 420 nm, Injection volume 10 μL

[0035] As shown in Figure 3, the three main peaks of the basic turmeric extract represent, in order, bis-demethoxycurcumin (bdmc), demethoxycurcumin (dmc), and curcumin (ccm) [diferuloylmethane].

[0036] Example 4: Analysis of Curcumin Content in Basic Turmeric Extract

[0037] The curcumin content of the basic turmeric extract was analyzed by commissioning the Korea Quality Testing Institute (KQT).

[0038] The details of the sample pretreatment are as follows:

[0039] ① The sample stored in a refrigerated state was taken out and left at room temperature 2 hours before the test was performed so that the temperature of the sample was similar to the laboratory temperature.

[0040] ② After homogenizing the sample by shaking, 1 mL was taken and added to 74 mL of ultrapure water. The total volume was 74 mL.

[0041] ③ After shaking and mixing to ensure the solute is homogeneous, the solution was filtered through a 0.45 μm PTTE syringe filter to obtain the test solution.

[0042] ④ The test solution was placed in a 2 mL vial, mounted on an autosampler, and a certain amount was injected for instrumental analysis.

[0043] The analysis conditions are presented in Table 3 below.

[0044] Instruments: Shimadzu HPLC Column: Shim-pack GIS, (250 x 4.6) mm, 5 μm Detector: PDA Wavelength: 425±4 nm Column Temperature: 35℃ Flow rate: 1.0 mL / min Injection volume: 50 μL Mobile phase: A: 0.025M KH2PO4 (pH 4.0) B: Acetonitrile gradient Recording Time: 35 minutes

[0045] As a result of the analysis, the curcumin content of the basic turmeric extract was 0.28 mg / L.

[0046] Example 5: Measurement of Anions, Emissivity, and Radiation Energy of Basic Turmeric Extract

[0047] The negative ions, emissivity, and radiant energy of the basic turmeric extract were measured by commissioning the Korea Far Infrared Application Evaluation Research Institute, affiliated with the Korea Far Infrared Association.

[0048] Using a charged particle measuring device, a test was conducted to measure the number of negative ions under conditions of an indoor temperature of 25℃, humidity of 55%, and an atmospheric negative ion count of 126 / cc. The negative ions emitted from the subject were measured and expressed as the number of ions per unit volume, and the number of negative ions was 140 / cc.

[0049] The emissivity and radiant energy measured relative to BLACK BODY using an FT-IR spectrometer at 37°C for cotton samples immersed in basic turmeric extract are shown in Table 4 and Figures 2 and 3 below.

[0050] Emissivity (5~20 µm) Radiant Energy (W / m² µm, 37℃) 0.867 3.34 × 10⁻⁶ 2

Claims

1. (a) A step of introducing water into a tank having an air circulation passage at the top and a silicon-based mineral plate containing mica and coarse-grained stone arranged on the bottom with a magnet having a magnetic flux density (magnetic flux density) of 10,000 Gauss or more, and introducing and mixing mica powder and shellfish shell powder (including internal sand of shellfish shells), and stirring the mixed solution for 16 to 72 hours; (b) a step of transferring the solution generated in step (a) into the interior of a water heater at 48°C or lower, which is equipped with an air circulation passage at the top, and then stirring the solution for 12 to 36 hours while applying electrical energy through alternating current of 220V and 60Hz frequency; (c) sealing the air circulation passage provided at the top of the water heater to create a vacuum, then dissolving salt at a concentration of 0.85–0.95% in the solution produced in step (b), and stirring for 15–24 hours at atmospheric pressure and a temperature of 48°C or lower; (d) a step of adding turmeric powder, salt, and shellfish shell powder to the solution produced in step (c) and mixing, and then stirring the mixed solution for 1 to 3 hours at atmospheric pressure and a temperature of 48°C or lower to produce a basic turmeric extract; (e) a step of aging the basic turmeric extract produced in step (d) above at room temperature for 15 to 18 days; and (f) A method for preparing a basic turmeric extract comprising the step of maintaining the basic turmeric extract aged in step (e) under vacuum for 9 to 11 hours to obtain a basic turmeric extract.

2. In Paragraph 1, A method for preparing a basic turmeric extract in which the weight ratio of mica powder to fish shell powder in step (a) is 7:

3.

3. In Paragraph 1, A method for preparing a basic turmeric extract, wherein the above-mentioned shellfish powder is a shellfish powder selected from the group consisting of coral reefs, oysters, and scallops.

4. In Paragraph 1, A method for preparing a basic turmeric extract in which, in step (d) above, the mixed solution consists of 92-94% (w / v) of solution, 3-5% (w / v) of turmeric powder, 1.25-1.78% (w / v) of salt, and 0.25-0.35% (w / v) of fish and shellfish powder.

5. In Paragraph 1, A method for preparing a basic turmeric extract in which the pH of the basic turmeric extract is 10 to 12.

6. A basic turmeric extract produced by the manufacturing method of any one of paragraphs 1 to 5, wherein Basic turmeric extract containing diferuloylmethane, demethoxycurcumin, and bis-demethoxycurcumin, with a pH of 10 to 12.

7. In Paragraph 6, Basic turmeric extract having a curcumin content of 0.28 mg / L.

8. In Paragraph 6, 9561 mg / L of Cl - , 13.9 mg / L of NO3 - , 334.9 mg / L of SO4 2- Basic turmeric extract containing 5684 mg / L Na, 817 mg / L Ca, 41 mg / L Mg, 1097 mg / L K and 8.1 mg / L Si.

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