Pipetting interface for microfluidic devices

Abstract

In some embodiments, a pipette interface can include a housing having a first end, a second end, and a transverse surface. The housing can include one or more protrusions extending from the second end towards the first end along the transverse surface, and a first inlet having a first opening at the first end and a second opening at the second end. The pipette interface can include a core disposed within the housing, the core having a first end, a second end, and a transverse surface. The core can include a second inlet having a first opening at the first end and a second opening at the second end. The core can include at least one alignment feature configured to align the first inlet and the second inlet such that the second inlet is downstream from the first inlet.

Description

PFT-00425PIPETTING INTERFACE FOR MICROFLUIDIC DEVICESCROSS-REFERENCE TO RELATED APPLICATION

[0001] This application claims the benefit of U.S. Provisional Application No. 63 / 749,139, filed January 24, 2025, which is hereby incorporated by reference in its entirety.BACKGROUND

[0002] Embodiments of the present disclosure relate to pipette interfaces for microfluidic devices.BRIEF SUMMARY

[0003] In some embodiments, a pipette interface can include a housing having a first end, a second end, and a transverse surface. The housing can include one or more protrusions extending from the second end towards the first end along the transverse surface, and a first inlet having a first opening at the first end and a second opening at the second end. The pipette interface can include a core disposed within the housing, the core having a first end, a second end, and a transverse surface. The core can include a second inlet having a first opening at the first end and a second opening at the second end. The core can include at least one alignment feature configured to align the first inlet and the second inlet such that the second inlet is downstream from the first inlet.

[0004] In some embodiments, the core comprises a flange at the second end of the core, the flange extending from the transverse surface of the core.

[0005] In some embodiments, the flange is circular.

[0006] In some embodiments, the first inlet and the second inlet are configured to receive a pipette tip.

[0007] In some embodiments, the second inlet is configured to form a first seal with the pipette tip.

[0008] In some embodiments, the at least one alignment feature comprises a keying boss extending from the transverse surface of the core and a slot formed in the housing, the slot configured to receive at least a first portion of the keying boss.Page 1 of 28FOLEYHO AGUS 12700946.3PFT-00425

[0009] In some embodiments, the at least one alignment feature further comprises a groove formed in an interior surface of the housing, the groove configured to receive at least a second portion of the keying boss.

[0010] In some embodiments, the first inlet includes a first taper extending from the first opening towards the second opening of the first inlet and an interior volume configured to receive the core, the first taper extending into the interior volume.

[0011] In some embodiments, the first taper is conical.

[0012] In some embodiments, the pipette interface further comprises a seal protection shroud disposed between the first taper and the interior volume of the first inlet.

[0013] In some embodiments, the pipette interface further comprises a cap having a first side and a second side, defining a thickness therebetween. The first side can be disposed over the second end of the housing. The cap can comprise a third inlet extending through the thickness of the cap, the third inlet concentrically aligned with the second opening of the second inlet.

[0014] In some embodiments, the second side of the cap is welded to a surface of a microfluidic chip.

[0015] In some embodiments, the second inlet has a second taper.

[0016] In some embodiments, the second taper is conical.

[0017] In some embodiments, the core further comprises a gasket feature extending from the second end of the core, the gasket feature configured to compress against a surface of a microfluidic chip, thereby forming a second seal between the surface and the core.

[0018] In some embodiments, the gasket feature has a triangular cross-section.

[0019] In some embodiments, the housing is formed of a rigid polymer material.

[0020] In some embodiments, the core is formed of a flexible polymer material.

[0021] In some embodiments, the second end of the housing is welded to a surface of a microfluidic chip.

[0022] In some embodiments, the pipette interface further comprises a cap, wherein the microfluidic chip is disposed between the housing and the cap.

[0023] In some embodiments, the second inlet includes a first internal taper and a second internal taper, the first internal taper disposed towards the first end of the core and the second internal taper disposed towards the second end of the core, and the second internal taper being longer and steeper than the first internal taper.Page 2 of 28FOLEYHO AGUS 12700946.3PFT-00425

[0024] In some embodiments, the core includes a retention feature formed on an exterior surface of the core and configured to engage the housing to resist axial movement of the core relative to the housing.

[0025] In some embodiments, the retention feature of the core comprises a reverse taper feature.

[0026] In some embodiments, the housing includes a plurality of ribs extending inwardly from an interior surface of the housing, the plurality of ribs configured to engage an exterior surface of the core.

[0027] In some embodiments, the at least one alignment feature includes: a mating face formed at the first end of the core; and a gland formed in the housing, the gland configured to receive at least a portion of the mating face.

[0028] In some embodiments, the core is spaced from the housing to define a clearance therebetween that permits radial deformation of the core.

[0029] In some embodiments, a microfluidic chip can comprise a first side, a second side, at least one fluid via, and at least one fluidic channel in fluidic communication with the at least one fluid via. The microfluidic chip can further comprise the pipette interface described above affixed to the first side of the microfluidic chip.

[0030] In some embodiments, the fluid via includes a first opening disposed on the first side of the microfluidic chip, the second opening of the second inlet concentrically aligned with the first opening of the fluid via.

[0031] In some embodiments, a surface of the microfluidic device includes a tapered groove configured to receive a portion of the core.BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS

[0032] Fig. 1A illustrates a perspective view of an embodiment of a pipette interface attached to a microfluidic chip, in accordance with embodiments of this disclosure.

[0033] Fig. IB illustrates a top perspective view of an embodiment of an assembled pipette interface, in accordance with embodiments of this disclosure.

[0034] Figs. 1C and ID illustrate a top perspective view of an embodiment of pipette interfaces arranged in an array configuration, in accordance with embodiments of this disclosure.

[0035] Fig. 2A illustrates an exploded assembly view of an embodiment of a pipette interface including a housing and a core, in accordance with embodiments of this disclosure.Page 3 of 28FOLEYHO AGUS 12700946.3PFT-00425

[0036] Fig. 2B illustrates a cross-sectional side view of an embodiment of a pipette interface connected to a microfluidic chip showing interior features of the pipette interface, in accordance with embodiments of this disclosure.

[0037] Fig. 2C illustrates a bottom view of an embodiment of a pipette interface coupled to a fluid via in fluidic communication with a fluidic channel of a microfluidic device, in accordance with embodiments of this disclosure.

[0038] Fig. 3 illustrates a cross-sectional side view of an embodiment of a fully encapsulated pipette interface coupled to a microfluidic chip, in accordance with embodiments of this disclosure.

[0039] Figs. 4A and 4B illustrate cross-sectional views of pipette tips inserted through an opening of an embodiment of a pipette interface, in accordance with embodiments of this disclosure.

[0040] Fig. 5 illustrates a cross-sectional view of an embodiment of an assembled pipette interface, in accordance with embodiments of this disclosure.

[0041] Fig. 6 illustrates a view of an embodiment of a housing, in accordance with embodiments of this disclosure.

[0042] Fig. 7 illustrates a bottom view of an embodiment of an assembled pipette interface, in accordance with embodiments of this disclosure.

[0043] Figs. 8A and 8B illustrate partial cross-sectional views of an embodiment of an assembled pipette interface, in accordance with embodiments of this disclosure.DETAILED DESCRIPTION

[0044] Current microfluidic systems are often designed to handle and manipulate extremely small volumes of fluid — usually on the order of nanoliters to microliters — and must do so with great precision. While microfluidic chips are optimized for fluid routing, mixing, and analysis at the microscale, they frequently face a key challenge: interfacing with the macroscopic world. Researchers and technicians commonly introduce samples and reagents using handheld pipettes. However, the transition from the large-bore of a pipette tip to the microfluidic channel is often a point of difficulty. Ensuring a tight seal, precise alignment, and minimal dead volume while preventing contamination at this interface can be challenging. There thus remains a need for aPage 4 of 28FOLEYHO AGUS 12700946.3PFT-00425stable, reproducible interface on the chip itself, where standard pipette tips can reliably connect without leaks or alignment issues.

[0045] Transitioning to an automated pipetting robot exacerbates these problems because most available robots for this use do not have the same dexterity as a human user. Automation is a key driver in modern laboratory workflows, particularly in industries and research areas like high-throughput screening, genomics, drug discovery, and diagnostics. Automation reduces human error, and a consistent chip interface ensures that every fluid transfer is performed identically. Reproducibility improves with a stable interface and fixed coordinates. Data from automated assays tend to have lower variability and fewer outliers due to consistent sample handling.Beyond the initial fluid introduction, microfluidic chips can be integrated into full workflows involving automated mixing, incubation, optical detection, or other sensing steps. A robust pipetting interface is the first link in the chain, ensuring that the starting conditions are as controlled and repeatable as the rest of the automated process.

[0046] In automation, hands-on supervision can be limited; therefore, robust sealing between the pipette tip and the microinlet is needed. A well-designed pipetting interface that securely mates with standard pipette tips can prevent leaks, ensuring stable fluid handling. Automation thrives on reproducibility and reduced manual intervention. Having a well-integrated pipetting interface on a microfluidic chip can allow for a robot that does not need additional adapters, tubing, or complex alignment fixtures. The chip can be placed in a holder or a stage that the robot recognizes, and fluids can be introduced directly, simplifying the workflow.

[0047] A common format for products used in automated laboratory systems is defined by the Society for Laboratory Automation and screening. These products, commonly called microplates, are the basis for many high-throughput laboratory processes. However, many of the existing solutions for pipette interfaces are too large or require spacing that is too far apart to be compatible with common microplate well-spacing dimensions such as 4.5mm or 9mm from center to center.

[0048] A number of microfluidic chips exist that have a molded inlet port or barbed connector that is designed to mate with tubing. While these ports and connectors can eventually be connected to a pipette through an intermediate piece of tubing or specialized adapter, they do not typically allow direct pipette tip insertion. Additional adapters or tubing connections can be used, but these increase the complexity of the workflow because users must carefully align channelsPage 5 of 28FOLEYHO AGUS 12700946.3PFT-00425and ensure that the adapters or connectors are properly seated, which is time-consuming and prone to error. They can also introduce extra fluid volume that is not efficiently delivered into the chip, wasting precious reagents and samples. There are pipette- specific port designs integrated into the design of the microfluidic chip, but the lack of compliance of a rigid thermoplastic or glass device precludes the use of a pipetting robot. These solutions also only accommodate a specific model or small selection of pipette tips.

[0049] Soft-lithography-based microfluidic chips (commonly made of Polydimethylsiloxane (PDMS)) may incorporate open wells or reservoirs at the chip surface. A pipette tip can be pressed directly into these reservoirs to deliver fluid. Achieving a proper seal and controlling the exact fluid volume delivered can be difficult. These soft lithography-based microfluidic chips can be applied to a thermoplastic device and may work for human users, but a soft material can be difficult to use with a pipetting robot due to its relative inaccuracy. Any misalignment between the pipette tip and device inlet can lead to catching the soft material which can then occlude or contaminate the fluid path. Insertion of the pipette tip in a hole in PDMS can also create debris that can be propagated in the chip and alter the functionality of the chip or clog it.

[0050] Machined adapter blocks with O-rings or gaskets that align a pipette tip to a chip inlet also exist. While these adapters can improve reproducibility, they are bulky, expensive to fabricate, and not readily integrated into the chip itself. Much like the simpler additional tubing solution, significant dead volume is added in using these adapters.

[0051] Vendors offer specialized pipette tips designed for microfluidic applications. These tips may have finer, tapered ends or integral fittings that mate with micro-channels. However, switching to specialized tips adds cost, requires supply-chain reliability, and may limit flexibility. These tips also limit robot compatibility as most robots accept a small selection of pipette tips, often limited to only those from their own manufacturer.

[0052] As evident from the related art, conventional methods and systems fail to offer a seamless, reliable, cost-effective and scalable way to interface standard pipette tips directly to microfluidic chips. Achieving a reliable seal is difficult, especially with a robotic pipetting system. Additional hardware outside of the microfluidic device is required, adding complexity and dead volume or limiting compatibility with a broad set of available pipette tips. Scalability and integration into a mass produceable or highly parallelized chip format is not feasible due to cost or complexity. Finally, a majority of the solutions also require some level of humanPage 6 of 28FOLEYHO AGUS 12700946.3PFT-00425disassembly to remove the microfluidic device from a pipetting robot, limiting the types of automated workflows that can be completed by the robots without significant human intervention. The challenges described above have prevented the adoption of microfluidic devices in high throughput laboratory applications.

[0053] Automating pipetting in microfluidics can benefit from carefully engineered interfaces that address alignment, sealing, and scalability challenges. By enabling direct, robot-friendly pipetting of fluids into microfluidic chips, these interfaces streamline workflows, reduce human labor, enhance data quality, and substantially increase throughput. As more laboratories turn to automation, the value of well-designed pipetting interfaces will only continue to grow.

[0054] The disclosed subject matter includes microfluidic devices compatible with existing laboratory automation systems. The pipette interface can be designed to provide a robust, reliable, and cost-effective method for adapting microfluidics to lab automation systems without the need for customization of existing pipetting robots.

[0055] In some embodiments, the pipette interface is configured to interface between a pipette tip and microfluidic devices. The pipette interface includes a flexible core (e.g., elastomeric core) within a rigid polymer housing, configured to securely receive and form a seal with pipette tips of varying sizes. The interface can minimize dead volume, ensuring optimal performance with larger pipette tips, while gradually reducing dead volume for smaller tips. This feature can be particularly beneficial when handling small fluid volumes, as it maximizes sample retention and minimizes losses during transfer, improving precision in microfluidic applications.

[0056] In some embodiments, the rigid housing includes a lead-in surface such that a pipette tip introduced into the rigid housing can glide along the surface without risk of catching, which can facilitate working with automated pipetting systems. The rigid housing can incorporate guiding features that can be configured for robotic systems capable of handling 384- well microplates, a common format used in automated laboratory systems. A keying feature molded onto the exterior of the housing can ensure precise alignment during assembly, thereby maintaining the necessary center-to-center spacing to match the microplate configuration. For example, the keying feature can be configured to maintain a 4.5 mm center-to-center spacing to emulate a 384-well microplate. In some embodiments, pipette interfaces can be arranged for simultaneous mating with multi-tipped pipetting systems.Page 7 of 28FOLEYHO AGUS 12700946.3PFT-00425

[0057] The pipette interfaces can be installed on the molded microfluidic devices after initial fabrication using a secondary method. In this way, one or more pipette interfaces can be placed anywhere on the surface of the microfluidic device, which is distinct from existing solutions that are molded in place during initial fabrication. In some embodiments, installing pipette interfaces on a grid that matches existing laboratory standards can be advantageous.

[0058] The core of the pipette interface can allow interference between it and the pipette tip, forming a releasable leak-proof seal. The seal can be configured to withstand over 6 bar of pressure at the joint without leaking. This pressure tolerance can accommodate available automated liquid handling systems. The same pipette interface can also be used for connecting a microfluidic device to standard capillary tubing commonly employed in microfluidic systems.

[0059] The core can include an inner taper configured to release the pipette tip when the automated system retracts without causing the pipette tip to be removed from the pipetting head of the automated system. In conventional systems and methods, the pipette tip may become stuck in the microfluidic device, preventing successful retraction of the automated system. However, in some embodiments of the present disclosure, the releasable seal allows for repeated connection and disconnection of the pipette tip without functional damage to the seal within a number of cycles commonly used in lab applications. For example, in some embodiments of the present disclosure, the pipette interface can be designed to withstand over 100 connections and disconnections without noticeable degradation in performance. In contrast, conventional systems may only support one initial connection before performance begins to deteriorate.

[0060] In some aspects, the core and housing can be removably coupled to each other. In some aspects, separable components allows for wider material compatibility combinations. For example, the housing can be formed of the same material as the component to which it is attached or can be made of a different material (e.g., more economical material). The core can be formed of application specific wetted materials to ensure compatibility with a wide range of operating temperatures and chemicals. The core can be mechanically coupled to the housing. In some aspects, the mechanical coupling allows the core and the device surface (e.g., microfluidic device surface) to be formed of chemically incompatible materials, as no chemical bonding is used for their connection. The mechanical coupling can simplify integration into a variety of microfluidic chip configurations and can make it adaptable to multiple applications.Page 8 of 28FOLEYHO AGUS 12700946.3PFT-00425

[0061] Conventional systems often require manual assembly of several components, driving up device cost. The pipette interface can be configured for automated assembly with high volume manufacturing techniques such as laser welding, reducing cost for customers when they move their products to mass production. The low-cost nature of the pipette interface can allow for single-use devices, which can help to ensure accuracy of results in a device by eliminating risks associated with multiple uses, such as re- sterilization and washing of devices.

[0062] The pipette interface can be formed of one or more materials (e.g., a combination thereof), including, but not limited to, Cyclic Olefin Polymer, Cyclic Olefin Copolymer, silicone rubber, fluorosilicone, thermoplastic elastomers, polydimethylsiloxane, polyurethane, polymethyl methacrylate, polycarbonate, polystyrene, polypropylene, polyethylene, polyethylene terephthalate, polyetherimide, polytetrafluoroethylene, glass, and polyvinyl chloride. For example, the housing of the pipette interface can be formed of Cyclic Olefin Polymer and the core can be formed of Cyclic Olefin Copolymer. The pipette interface can be formed of materials that are compatible with gamma sterilization, ethylene oxide (ETO) sterilization, electron beam sterilization, and other industry standard sterilization methods.

[0063] The base surface of the pipette interface can be pretreated with a laser absorbing additive that allows absorption of laser energy only at the surface where the pipette interface bonds to the device surface. The pre-treatment can allow users to use pick and place style assembly techniques to install pipette interfaces in correct positions without the work-time limitations and risk of accidental contamination associated with adhesive-based techniques.

[0064] The various concepts introduced above and discussed in greater detail below may be implemented in a number of ways, as the described concepts are not limited to any particular manner of embodiment.

[0065] Reference will now be made in detail to the exemplary embodiments of the present disclosure, examples of which are illustrated in the accompanying drawings. Wherever possible, the same reference numbers will be used throughout the drawings to refer to the same or like parts.

[0066] References herein to positions of elements (e.g., “top”, “bottom”) are merely used to describe the orientation of various elements in the FIGURES. It should be noted that the orientation of various elements may differ according to other embodiments, and that such variations are intended to be encompassed by the present disclosure.Page 9 of 28FOLEYHO AGUS 12700946.3PFT-00425

[0067] The term “inlet” and as used herein, generally refers to an aperture, orifice or channel extending through at least a portion of a thickness of the component (e.g., pipette interface).

[0068] The term "about" means a range of values inclusive of the specified value that a person of ordinary skill in the art would reasonably consider to be comparable to the specified value. In some embodiments, "about" means within a standard deviation using measurements generally accepted by a person of ordinary skill in the art. In some embodiments, "about" means ranging up to ±10% of the specified value. In some embodiments, "about" means ranging up to ±5% of the specified value. In some embodiments, "about" means the specified value.

[0069] Fig. 1A is a perspective view 100 of a pipette interface attached to a microfluidic chip according to embodiments of the present disclosure. Fig. IB is a top perspective view 150 of an assembled pipette interface according to embodiments of the present disclosure. Referring now to Figs. 1A and IB, the pipette interface 101 includes a flexible core 102 configured to form a seal with the chip and a rigid housing 104 that protects the core while connecting it to the microfluidic chip 106. In some embodiments, the pipette interface 101 includes a housing 104 and a core 102 disposed within the housing. In some embodiments, the pipette interface 101 includes a series of housings 104 (e.g., interconnected housings) with a single core 102. In some embodiments, the core 102 is integral with the housing 104. For example, the core may be overmolded with the housing 104. In some embodiments, the core 102 and the housing are separable parts.

[0070] In some embodiments, the housing 104 includes multiple housings 104. In some embodiments, the housing 104 is configured to hold multiple cores 102. For example, a pipette interface 101 may include a row, column, or a grid of locations, with spacing that matches existing laboratory standards (e.g., 9mm or 4.5mm from center-to-center), where a housing 104 is positioned at each location with a core 102 disposed in the housing. This pipette interface assembly can minimize costs by decreasing the number of discrete components required for assembly. Compact spacing may be required of pipette interfaces on a microfluidic chip for custom configurations or to match that of a row, column, or full grid of a 384-well or 96-well microplate. Figs. 1C and ID are top perspective views 170, 190 of pipette interfaces arranged in arrays corresponding to standard well plates: a 384-well plate with 4.5 mm center-to-center spacing (Fig. 1C) and a 96-well plate with 9 mm center-to-center spacing (Fig. ID).Page 10 of 28FOLEYHO AGUS 12700946.3PFT-00425

[0071] The housing 104 may include one or more alignment features to orient or fixture the housing relative to other housings to achieve the required spacing. These features can also be used to align a mating component or connector during manufacturing, testing, or end use of the microfluidic chip. The alignment features can include positive features such as pins, protrusions, tabs, flanges, raised elements on the housing and / or negative features such as holes, recesses, slot configured to receive the positive features. The alignment features can include ridges, rails, and / or visual markers (e.g., alignment marks, engraved lines). Embodiments of these alignment features may include variations in shape, quantity, and / or position of the feature. In some embodiments, the one or more alignment features are integral with the housing 104. In some embodiments, the one or more alignment features are separate from the housing 104.

[0072] The core 102 can fit inside of the housing 104, forming a subassembly that can be attached to a microfluidic chip 106 through an additional assembly process. The microfluidic chip 106 can include one or more fluidic channels 108, which may converge at a single fluid via or each lead to its own individual fluid via. The assembled pipette interface 101 (e.g., as shown in Fig. IB) can be positioned on the fluid via 110 on the microfluidic chip 106. The fluid via can be routed through a hole in the cap 112 of the microfluidic chip, in which case the pipette interface 101 can be attached to the cap 112 of the microfluidic chip. There may be one or more assembled pipette interfaces 101 disposed on the microfluidic chip 106 and / or the cap 112 of the microfluidic chip, facilitating fluidic communication between the microfluidic chip and the pipette interface(s). The housing 104 can include inlet 204 having a lead-in surface that tapers towards an interior volume (e.g., a cavity) that is configured to receive the core 102. The housing 104 can include an assembly keying boss 122 that is configured to receive keying bosses of the core 102.

[0073] The core 102 can be formed of an elastomeric thermoplastic such as Cyclic Olefin Copolymer elastomer, Styrene-Ethylene-Butylene-Styrene (SEBS) elastomer, thermoplastic urethane, rubber, fluoroelastomer, or like flexible materials. The housing 104 can be formed of the same material as the microfluidic chip so that it can be welded or bonded onto the device. For example, the housing 104 can be formed of polydimethylsiloxane, glass, polycarbonate, Cyclic Olefin Polymer, polystyrene, polymethyl methacrylate, and / or silicon.

[0074] Fig. 2A is an exploded assembly view 200 of a pipette interface including a housing and core, in accordance with embodiments of the present disclosure. Fig. 2B is a cross-sectional sidePage 11 of 28FOLEYHO AGUS 12700946.3PFT-00425view 230 of a pipette interface connected to a microfluidic chip showing guiding and sealing features of the pipette interface, in accordance with embodiments of the present disclosure. The cap 112 of the microfluidic chip is disposed beneath the microfluidic chip 106, which includes the fluid via 110 that is in fluidic communication with the fluidic channel 108. Referring now to Figs. 2A and 2B, the rigid housing 104 can include a body 202 (e.g., cylindrical body) with an inlet 204 having a first opening 206 (e.g., circular opening) disposed on a first end of the body and a second opening 232 (e.g., circular opening) disposed on the second end of the body 202. The exterior surface of the body 202 can taper from the first end to the second end. The first opening 206 of the housing 104 has a larger cross-sectional area than the second opening 232. The first opening 206 can be the same shape as the second opening 232. The inlet 204 can be referred to as a lead-in surface. An assembly keying boss 122 can receive keying bosses of the core 102, as described further below.

[0075] The housing 104 can include an interior volume 233 configured to receive the core 102. For example, a first portion of the interior volume 233 can be configured to receive the body 210 of the core 102, while a second portion of the interior volume 233 can be configured to receive the flange 218 of the core 102. The interior volume 233 can be larger than the core 102, such that a gap, or housing core-clearance, is formed between the exterior surface of the core 102 and the interior of the housing 104 in the assembled configuration. In this way, the gap can provide space for the core 102 to deform when a pipette tip is inserted. The inlet 204 can include one or more tapered sections and one or more straight sections (i.e., sections with a constant diameter) between the first opening 206 to the second opening 232. The one or more tapered sections and one or more straights sections can be positioned upstream from the interior volume configured to receive the core 102. For example, the inlet 204 can include a first taper 234 followed by a straight section. The first taper 234 has a gradual reduction in cross-sectional area, transitioning from a larger cross-sectional area at the first opening 206 to a smaller cross-section area. In some embodiments, the first taper is symmetric. For example, the first taper 234, or hard lead-in surface, can be a conical surface that serves as a guide for a pipette tip inserted through the first opening 206. In some embodiments, the first taper 234 is axisymmetric. In some embodiments, a pipette tip is inserted through the first opening 206 of the housing 104 without contacting the surface of the taper. In some embodiments, a pipette tip is inserted through the first opening 206Page 12 of 28FOLEYHO AGUS 12700946.3PFT-00425and contacts the surface of the taper, allowing the tip to glide along the surface and be guided to the interior of the pipette interface.

[0076] The inlet 204 can include, or be connected to, a seal protection shroud 235. In some embodiments, the seal protection shroud 235 is an O-ring, a gasket, or a membrane. In some embodiments, the seal protection shroud 235 is disposed over the first opening 206 of the housing 104. The seal protection shroud 235 can be shaped and sized to cover (e.g., circumferentially cover) the first opening 206 of the housing 104. In some embodiments, the seal protection shroud 235 is positioned between the first and second opening of the inlet. For example, the seal protection shroud 235 can be positioned within a straight section of the inlet 204. In some aspects, the seal protection shroud 235 provides a physical barrier that prevents external elements (e.g., contaminants) from entering the interior of the pipette interface. In some aspects, the seal protection shroud 235 provides a physical barrier that reduces a loss of liquid by evaporation. The seal protection shroud 235 can be formed of one or more materials, including but not limited to, flexible materials such as rubber and silicone. The seal protection shroud 235 can allow for the introduction of liquids while maintaining an airtight and / or leak-proof seal. In some embodiments, a pipette tip or needle tip can penetrate through the thickness of the seal protection shroud 235. In some embodiments, the seal protection shroud 235 is configured to reseal once the tip is removed.

[0077] The body 202 of the housing 104 can include one or more slots 208 and / or one or more grooves 207, each configured to receive at least a portion of one or more keying bosses 216 of the core 102. These features ensure correct orientation during assembly and prevent misalignment between the core 102 and housing 104. The engagement of the keying bosses 216 with the slots 208 and / or grooves 207 restricts relative motion, such as rotation, between the core 102 and housing 104. The grooves 207 can be formed on the interior of the housing, positioned above each corresponding slot 208. In the assembled configuration (i.e., coupled housing and core), the one or more keying bosses 216 of the core 102 may protrude from the slots 208 (e.g., as shown in Fig. IB).

[0078] In some embodiments, the housing 104 and the core 102 are coupled using a fastening mechanism (e.g., screw connection, bolted connection). In some embodiments, the housing 104 and the core 102 are coupled using opposing magnetic contacts, mating joints, and / or a snap-fit mechanism (e.g., snap-fit joints).Page 13 of 28FOLEYHO AGUS 12700946.3PFT-00425

[0079] The body 202 of the housing 104 can include one or more protrusions 209 extending longitudinally along the transverse surface (e.g., a surface other than an end surface, which can include a curved or lateral surface extending generally along a longitudinal axis of the component) from the second end of the body towards the first end of the body. In some aspects, the one or more protrusions 209 increase the surface area of the bottom surface of the pipette interface, which is welded to the surface of the device.

[0080] Still referring to Figs. 2A and 2B, the flexible core 102 can include a body 210 (e.g., cylindrical body) with an inlet 212 having a first opening 214 (e.g., circular opening) disposed on a first end of the body and a second opening 236 (e.g., circular opening) disposed on the second end of the body. The exterior surface of the body 210 can taper from the first end to the second end. The first opening 214 of the flexible core 102 has a larger cross-sectional area than the second opening 236. The first opening 214 can be the same shape as the second opening 236.

[0081] The inlet 212 can taper from the first opening 214 to the second opening 236. In some embodiments, the inlet 212 includes a first taper and a second taper. The first taper has a gradual reduction in cross-sectional area, transitioning from a larger cross-sectional area at the first opening 214 to a smaller cross-sectional area. The second taper, which follows the first taper, continues to reduce the cross-sectional area from the smaller cross-sectional area of the first taper. The first taper and the second taper can have different rates of taper. In some embodiments, the shape of the taper (e.g., the second taper) is configured to follow the contour of the exterior surface of a pipette tip, such that the taper gradually reduces in cross-sectional area in a manner consistent with the tapering geometry of the pipette tip. In some embodiments, the rate of taper of the second taper is different from the taper of the pipette tip. In some embodiments, the first taper and / or the second taper of the inlet 212 is conical. In some embodiments, the inlet 212 can be referred to as a pipette tip sealing cone.

[0082] The second opening 236 of the flexible core 102 can have a geometry that matches the contours of an opening of the fluid via 110. For example, the second opening 236 can be a circular opening that circumferentially aligns with a circular edge of an opening of the fluid via 110, ensuring a seamless interface between the opening and the fluid path.

[0083] The body 210 of the core 102 can include one or more keying bosses 216 extending longitudinally along its transverse surface. For example, a pair of keying bosses 216 can be positioned around the edge of the body 210 and on opposite sides. In another example, a pair ofPage 14 of 28FOLEYHO AGUS 12700946.3PFT-00425keying bosses 216 can be positioned around the circumference of a cylindrical body 210 and diametrically opposing each other. In some embodiments, other amounts of keying bosses 216 can be employed and disposed around the edge of the body 210. The one or more keying bosses 216 are protrusions configured to engage with a corresponding slot 208 and / or groove 207 on the housing 104. The one or more keying bosses 216 can be dimensioned and shaped to ensure a precise fit within the slot 208 and / or groove 207. The one or more keying bosses 216 may have a cylindrical, rectangular, or tapered shape.

[0084] The body 210 can include a flange 218 extending (e.g., extending radially) from the exterior surface of the body, perpendicular to the longitudinal axis of the body. The flange may be a circular flange, for example, as shown in Fig. 2A. The flange 218 can include a gasket feature 238 (e.g., a tongue or protrusion). In some embodiments, the gasket feature 238 is configured to fit within a complementary recess that surrounds the fluid via 110. In some embodiments, the gasket feature 238 is configured contact the planar surface of the device. The body 210 can include a protrusion 240 that extends from the bottom surface of the body 210. A gap can be formed between the gasket feature 238 and the exterior surface of the protrusion 240 when the core 102 is positioned on the surface of the chip.

[0085] The core 102 can be sealed between the chip and housing 104 by means of the gasket feature 238. In some embodiments, the gasket feature 238 is integral with the core 102. In some embodiments, the gasket feature 238 is separate from the core. The gasket feature 238 can be a compressible seal that traces the perimeter of the core 102 and isolates the fluid path between the end of the pipette tip and the chip. The width, height, and shape of this feature can be configured to provide a specific amount of pressure when compressed against the microfluidic chip during assembly. This compressive force can be provided by the elastic behavior of the material; the deformation of the material acts like a uniform spring along the perimeter of the core 102. The gasket feature 238 may have a rounded, rectangular, or triangular cross-section. A triangular cross-section with its pointed tip oriented towards the chip surface may provide the most of compliance. This triangular cross-section may be suitable for elastomeric materials with a high compression set, as the pointed tip of the triangular profile allows for sufficient collapse to account for permanent deformation and gradual displacement of material after assembly.

[0086] Fig. 2C is a bottom view 250 of a pipette interface coupled to a fluid via 110 in fluidic communication with a fluidic channel 108 of a microfluidic device, in accordance withPage 15 of 28FOLEYHO AGUS 12700946.3PFT-00425embodiments of the present disclosure. The one or more protrusions 209 define planar surfaces 252, which serve as welding surfaces 256 to attach the housing 104 to the device. In some embodiments, a portion of the bottom surface of the core 102 (e.g., gasket feature 238) forms a surface seal 254 with the surface of the chip, with the seal encircling the opening of the fluid via 110. In some embodiments, the bottom surface of the core 102 is planar and forms a seal with the surface of the chip.

[0087] The housing 104 can be coupled to the device in a number of ways. For example, the housing 104 can be bonded to the device by means of a laser welding process or other alternative thermoplastic welding methods such as ultrasonic welding and diffusion bonding. Laser welding can minimize the heat produced during welding and allows for better part alignment and tolerance management. The housing 104 can be coupled to the device by means of a snap-fit feature, a bolted connection, a magnetic connection, or an adhesive. The housing 104 and device can be clamped together with an additional component or subassembly. The coupling of the housing 104 to the device compresses the gasket feature 238 in the core 102.

[0088] Fig. 3 is a cross-sectional side view 300 of a fully encapsulated pipette interface 101 connected to a microfluidic chip, in accordance with embodiments of the present disclosure. The cap 112 of the microfluidic chip is disposed beneath the microfluidic chip 106, which includes the fluid via 110 that is in fluidic communication with the fluidic channel 108. Referring now to Fig. 3, in some embodiments, the core 102 is fully encapsulated within the interior volume 233 of the housing, or interface housing, 104 by a cap 302. In the assembled configuration, with the core 102 disposed within the housing 104, the cap 302 can be positioned over the second end of the body 202, extending over the second opening 232 of the housing. The cap 302 has an upper surface (e.g., planar surface) and a lower surface (e.g., planar surface), defining a thickness therebetween. The cap 302 (or interface housing cap) includes an inlet 304 with a first opening 306 disposed on the upper surface and a second opening 308 disposed on the lower surface of the cap. The inlet 304 can have a constant diameter along the thickness of the cap 302. The cap 302 can be cylindrical, rectangular, or triangular. The cap 302 can be shaped such that, in the assembled configuration, the outermost edge of the bottom surface of the housing 104 aligns with an edge of the cap 302 (e.g., the outermost edge of the lower planar surface of the cap). In the assembled configuration, the bottom surface of the core 102 and of the housing 104 contact the upper surface of the cap 302, while the surface of the chip 106 (or microfluidic chip) contactsPage 16 of 28FOLEYHO AGUS 12700946.3PFT-00425the lower surface of the cap 302. The second opening 236 of the core 102 can be concentrically aligned with the first opening 306 of the cap 302, while the opening of the fluid via 110 can be concentrically aligned with the second opening 308 of the cap 302. The lower surface of the cap 302 can be coupled to the chip by means of thermoplastic welding processes, a snap-fit feature, a bolted connection, a magnetic connection, or an adhesive The cap 302 and device can be clamped together with an additional component or subassembly. The cap 302 can compress the gasket feature 238 of the core 102.

[0089] The microfluidic chip 106 and / or the cap 112 of the microfluidic chip can include one or more recesses configured to receive the pipette interfaces 101. In this way, the housing 104 may be recessed into the device to optimize space utilization. In some embodiments, the housing 104 is surrounded by a raised web of material. In some embodiments, the housing 104 is contained in the chip as part of a multi-layer stack.

[0090] Figs. 4A and 4B are cross-sectional views 400, 450 of different- sized pipette tips inserted through an opening of a pipette interface, in accordance with embodiments of the present disclosure. The housing 104 and core 102 are designed to guide and seal multiple sizes of pipette tips. The first taper 234 of the housing 104 defines an interior hard surface that can deflect the sharp edge of a pipette tip as it is inserted through the first opening 206 of the inlet 204 of the housing 104. The first taper 234 guides the pipette toward the longitudinal axis of the pipette interface 101. As the pipette tip nears the narrowest point of the first taper 234, a seal protection shroud 235 serves as a protective surface to prevent the sharp edge of the pipette tip from catching on and deflecting the core 102 into the fluid path. In some aspects, the first taper 234 and / or the seal protection shroud 235 align the pipette tip vertically, ensuring that the longitudinal axis of the pipette tip is colinear with the longitudinal axis of the pipette interface 101. This alignment ensures that the pipette tip advances through the first opening 214 of the inlet 212 of the core 102 without damaging the pipette tip and / or disrupting the core 102. The pipette tip is advanced until reaches a depth at which it interferes with the core 102, forming a pressure-resistant seal. Different sized pipette tips (e.g., pipette tips 402, 452) may need to be inserted to different depths to form a seal. For example, a smaller diameter pipette tip can be advanced further into the pipette interface 101 compared to a larger diameter pipette tip, for example, as shown in Figs. 4A and 4B. The distance from the tip of the pipette tip to the second opening 236 of the inlet 212 of the core 102 may vary depending on the size of the pipette tip.Page 17 of 28FOLEYHO AGUS 12700946.3PFT-00425

[0091] Repeated interference over numerous pipette tip insertions is supported through controlled deflection of the sealing surface. The deflection of the sealing surface can be controlled through design features including the shape of the sealing cone clearance in the housing for the core to stretch and the durometer of the core’s selected material.

[0091] In some embodiments, repeated interference over numerous pipette tip insertions is supported through controlled deflection of the core 102. The deflection of the core 102 can be controlled through design features including the shape of the housing core-clearance in the housing for the core to stretch and the durometer of the core’s selected material.

[0092] The geometrical form of the inlet 212 of the core 102 (e.g., the second taper) and the first taper 234 of the inlet 204 of the housing 104 can be configured to interfere sufficiently with the core to generate a strong enough seal before any other surface of the pipette tip contacts the housing, thus preventing further insertion into the pipette interface. The accuracy and precision of the pipette tip’s depth can vary, for example, depending on whether a robotic system or a manual pipette is used. The dimensions of the second taper of the inlet 212 may be adjusted to provide either a wider or narrower tip depth tolerance, or to accommodate a different selection of pipette tip sizes. Tubing may be inserted directly into the pipette interface. The mechanics of creating a seal with tubing, through interference against its outer surface, are similar to those used for creating a seal against a pipette tip.

[0093] Fig. 5 illustrates a cross-sectional view 500 of an assembled pipette interface 101, in accordance with embodiments of the present disclosure. Referring now to Fig. 5, the pipette interface 101 includes the rigid housing 104 (including body 202 and inlet 204) and the flexible core 102 disposed within the interior volume 233 of the housing 104. In some embodiments, the pipette interface 101 employs an enlarged or taller flexible core 102 relative to other embodiments (e.g., embodiments shown in Figs. 2A - 4B). In some aspects, an enlarged or taller flexible core 102 can increase the range of compatible pipette tip geometries while maintaining a robust seal and can allow for a wider tolerance for the pipette tip insertion depth. In some aspects, an enlarged or taller flexible core 102 can maintain a strong seal while minimizing the required extraction force upon tip removal, which may be an important consideration for automated liquid handling systems.

[0094] Still referring to Fig. 5, the housing inlet 204 includes the first opening 206 that leads into the interior volume 233 of the housing 104, and the housing 104 further includes a secondPage 18 of 28FOLEYHO AGUS 12700946.3PFT-00425opening 232 at the opposite end. The housing inlet 204 can include a lead-in taper configured to guide the pipette tip toward a longitudinal axis of the pipette interface 101. The flexible core 102 includes the body 210 having the inlet 212 with the first opening 214 and the second opening 236. The inlet 212 of the flexible core can include one or more internal tapers configured to receive a pipette tip and form an interference-based seal with the exterior surface of the pipette.

[0095] In some embodiments, the flexible core 102 includes two internal tapers, wherein one of the internal tapers is longer and steeper than the other. In some aspects, the longer and steeper taper allows the pipette tip to travel further into the sealing component to create a robust seal. In some embodiments, the assembled pipette interface 101 includes a housing core-clearance 252 (a clearance defined between the exterior surface of the flexible core 102 and the interior surface of the rigid housing 104) to accommodate the increased insertion depth and the resulting material deformation of the flexible core 102. In some aspects, this housing core-clearance can allow the flexible core 102 to expand radially outward when compressed by an inserted pipette tip without being forced against the interior surface of the housing 104, which may otherwise increase extraction force or lead to damage of the seal.

[0096] In some embodiments, the diameter of the first opening 206 of the housing inlet 204 is increased to accommodate the larger flexible core 102, which may only be feasible in embodiments employing an enlarged or taller assembly.

[0097] In some embodiments, the flexible core 102 is formed from a lower durometer, higher elasticity elastomer such that the flexible core 102 can undergo elastic deformation while maintaining sealing engagement with the pipette tip. A low-durometer core can also provide reduced force for extraction of the pipette tip. The compliance of the flexible core 102 can allow the bulk material of the seal to compress and deflect upon tip insertion which can be beneficial when the pipette tip is overly inserted, inserted at an angle, or when using tips with less predictable geometry due to manufacturing tolerances. In some embodiments, the flexible core 102 has a hardness in a range of about Shore 40A to about Shore 80A. In some embodiments, the flexible core 102 is formed from an elastomer such styrene-ethylene-butylene-styrene (SEBS), styrene-[ethylene-(ethylene-propylene)]-styrene (SEEPS), thermoplastic polyolefin (TPO), thermoplastic elastomer (TPE), thermoplastic vulcanizate (TPV), thermoplastic polyurethane (TPU), silicone, or another low durometer elastomer.Page 19 of 28FOLEYHO AGUS 12700946.3PFT-00425

[0098] An additional benefit of an enlarged or taller flexible core 102 is the ability to simplify the molding process. In some embodiments, smaller embodiments of the flexible core 102 may require side tabs or other features for demolding, while larger embodiments of the core may allow for ejector pins to be placed on a bottom surface of the component. This configuration can simplify tooling and improve manufacturing reliability.

[0099] In some embodiments, the flexible core 102 includes a retention feature 502. In some embodiments, the housing 104 includes a corresponding retention feature (e.g., a portion of the interior surface, a shoulder, a groove, a tapered or counter- tapered surface). In some embodiments, the retention feature of the flexible core 102 includes a reverse taper feature on an exterior surface of the flexible core. In some embodiments, the reverse taper feature increases in outer dimension towards the second end of the flexible core 102 and extends along a portion of a longitudinal length of the flexible core. In some embodiments, the reverse taper feature defines a localized outwardly flared region configured to engage the corresponding retention feature of the housing 104. In some embodiments, the reverse taper feature can be configured to retain the flexible core on a preferred side of the injection mold during cooling and mold opening. Due to the component's larger size and higher elasticity, the flexible core 102 can be reliably ejected without structural damage, thereby preserving the precision of the internal sealing taper geometry.

[0100] In some embodiments, the retention feature 502 (e.g., reverse taper feature) is configured to constrain the flexible core against the corresponding retention feature of the housing 104. This configuration can help maintain a clearance (e.g., a housing-core clearance) between the flexible core 102 and the housing 104 in regions surrounding the retention features. Alternatively, in some embodiments, the flexible core 102 has molded tabs that press up against the interior surface of the housing 104 to hold it in place. In some embodiments, molded tabs of the flexible core 102 may eliminate the need for retention features (e.g., one or more ribs) on the housing 104.

[0101] Fig. 6 illustrates a view 600 of a housing 104 of a pipette interface, in accordance with embodiments of the present disclosure. Fig. 7 illustrates a bottom view 700 of an assembled pipette interface, in accordance with embodiments of the present disclosure. Referring to Figs.6-7, in some embodiments, the housing 104 includes one or more ribs 602 (e.g., one, two, three, four, five, etc.). In some embodiments, the one or more ribs 602 extend inwardly from thePage 20 of 28FOLEYHO AGUS 12700946.3PFT-00425interior surface of the housing 104. In some embodiments, the one or more ribs 602 extend along at least a portion of a longitudinal length of the housing 104, for example from the second opening 232 toward the first opening 206. In some embodiments, the ribs 602 are circumferentially spaced and disposed around the second opening 232 of the housing, along the interior surface of the housing 104. In some embodiments, three or more bumps or ribs 602 extend inwards from the housing interior to interfere with the outer surface of the flexible core 102 (e.g., as shown in Fig. 7). The flexible core 102 can be press-fit into the housing 104 and held in place by this localized interference. This technique can minimize the inward deformation of the flexible core 102 in critical sealing regions, thereby preserving the required sealing characteristics.

[0102] Figs. 8A and 8B illustrate partial cross-sectional views 800, 850 of an assembled pipette interface, in accordance with embodiments of the present disclosure. Referring now to Figs. 8A and 8B, in some embodiments, the at least one alignment feature includes a mating face 802 (e.g., a contacting surface) at the top of the flexible core 102 and a gland 804 at an upper portion of the housing 104. In some embodiments, the mating face 802 includes an annular flat surface. In some embodiments, the pipette interface 101 includes a mating face 802 at the top of the flexible core 102 having an increased surface area relative to other embodiments. The mating face can serve multiple functions. For example, the mating face 802 can ensure proper seating of the flexible core 102 within the gland 804 at an upper portion of the housing 104 and can facilitate effective axial compression of the flexible core 102 in the assembled pipette interface 101. This geometry can ensure that the flexible core 102 is axially compressed within the housing 104 and can provide the necessary compressive force during assembly, such as during laser welding of the housing 104 to the microfluidic device. The mating face can also act as a primary locating feature at the top of the flexible core 102, which assists in guiding an inserted pipette tip into proper alignment with the inlet 212 of the flexible core 102.

[0103] In some embodiments, the gasket feature 238 located on the bottom of the flexible core 102, which forms the fluidic isolation barrier against the microfluidic chip 106, has a reduced diameter to decrease internal dead or wasted fluid volume. In some embodiments, the gasket feature 238 is dimensioned to provide a substantial reaction force when compressed during tip insertion. This stored mechanical energy can assist the automated system by applying a force to the pipette tip, the force causing the pipette to move out of the inlet 212 of the flexible core 102Page 21 of 28FOLEYHO AGUS 12700946.3PFT-00425during extraction, thereby reducing a required retraction force. In some embodiments, the gasket feature 238 at the bottom of the seal permits axial displacement of the flexible core 102 toward the fluidic interface by several millimeters while maintaining a fluid-tight seal. In some embodiments, the compressibility of the gasket feature 238 allows large-diameter pipette tips to form a seal against a generally flat face of the flexible core 102, which can reduce frictional engagement during pipette tip extraction.

[0104] In some embodiments, a mating surface of the microfluidic device 106 or the cap 112 includes a tapered groove. The tapered groove can be configured to receive a complementary portion of the flexible core 102 (e.g., the gasket feature 238), thereby maintaining or improving alignment of the core relative to the fluidic layer or bottom capping component during assembly.

[0105] In some embodiments, the retention features described herein may also function as alignment features configured to align the core with the housing.

[0106] The descriptions of the various embodiments of the present disclosure have been presented for purposes of illustration, but are not intended to be exhaustive or limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The terminology used herein was chosen to best explain the principles of the embodiments, the practical application or technical improvement over technologies found in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.Page 22 of 28FOLEYHO AGUS 12700946.3

Claims

PFT-00425CLAIMSWhat is claimed is:

1. A pipette interface, comprising:a housing having a first end, a second end, and a transverse surface, the housing including:one or more protrusions extending from the second end towards the first end along the transverse surface,a first inlet having a first opening at the first end and a second opening at the second end;a core disposed within the housing, the core having a first end, a second end, and a transverse surface, the core including:a second inlet having a first opening at the first end and a second opening at the second end; andat least one alignment feature configured to align the first inlet and the second inlet such that the second inlet is downstream from the first inlet.2 The pipette interface of Claim 1, wherein the core comprises a flange at the second end of the core, the flange extending from the transverse surface of the core.3 The pipette interface of Claim 2, wherein the flange is circular.The pipette interface of any one of Claims 1-3, wherein the first inlet and the second inlet are configured to receive a pipette tip.5 The pipette interface of Claim 4, wherein the second inlet is configured to form a first seal with the pipette tip.Page 23 of 28FOLEYHO AGUS 12700946.3PFT-004256. The pipette interface of any one of Claims 1-5, wherein the at least one alignment feature comprises:a keying boss extending from the transverse surface of the core; anda slot formed in the housing, the slot configured to receive at least a first portion of the keying boss.7 The pipette interface of Claim 6, wherein the at least one alignment feature further comprises a groove formed in an interior surface of the housing, the groove configured to receive at least a second portion of the keying boss.8 The pipette interface of any one of Claims 1-7, wherein the first inlet includes a first taper extending from the first opening towards the second opening of the first inlet and an interior volume configured to receive the core, the first taper extending into the interior volume.9 The pipette interface of Claim 8, wherein the first taper is conical.10 The pipette interface of Claim 8, further comprising a seal protection shroud disposed between the first taper and the interior volume of the first inlet.11 The pipette interface of any one of Claims 1-10, further comprising a cap having a first side and a second side, defining a thickness therebetween, the first side disposed over the second end of the housing, and wherein the cap comprises a third inlet extending through the thickness of the cap, the third inlet concentrically aligned with the second opening of the second inlet.12 The pipette interface of Claim 11, wherein the second side of the cap is welded to a surface of a microfluidic chip.13 The pipette interface of any one of Claims 1-12, wherein the second inlet has a second taper.Page 24 of 28FOLEYHO AGUS 12700946.3PFT-0042514. The pipette interface of Claim 13, wherein the second taper is conical.

15. The pipette interface of any one of Claims 1-14, wherein the core further comprises a gasket feature extending from the second end of the core, the gasket feature configured to compress against a surface of a microfluidic chip, thereby forming a second seal between the surface and the core.

16. The pipette interface of Claim 15, wherein the gasket feature has a triangular cross-section.

17. The pipette interface of any one of Claims 1-16, wherein the housing is formed of a rigid polymer material.

18. The pipette interface of any one of Claims 1-17, wherein the core is formed of a flexible polymer material.

19. The pipette interface of any one of Claims 1-10, 13-18, wherein the second end of the housing is welded to a surface of a microfluidic chip.

20. The pipette interface of any one of Claims 19, further comprising a cap, wherein the microfluidic chip is disposed between the housing and the cap.

21. The pipette interface of any one of Claims 1 to 20, wherein the second inlet includes a first internal taper and a second internal taper, the first internal taper disposed towards the first end of the core and the second internal taper disposed towards the second end of the core, and the second internal taper being longer and steeper than the first internal taper.Page 25 of 28FOLEYHO AGUS 12700946.3PFT-0042522. The pipette interface of any one of Claims 1 to 21, wherein the core includes a retention feature formed on an exterior surface of the core and configured to engage the housing to resist axial movement of the core relative to the housing.

23. The pipette interface of Claim 22, wherein the retention feature of the core comprises a reverse taper feature.

24. The pipette interface of any one of Claims 1 to 23, wherein the housing includes a plurality of ribs extending inwardly from an interior surface of the housing, the plurality of ribs configured to engage an exterior surface of the core.

25. The pipette interface of any one of Claims 1 to 24, wherein the at least one alignment feature includes:a mating face formed at the first end of the core; anda gland formed in the housing, the gland configured to receive at least a portion of the mating face.

26. The pipette interface of any one of Claims 1 to 25, wherein the core is spaced from the housing to define a clearance therebetween that permits radial deformation of the core.

27. A microfluidic chip, comprising:a first side;a second side;at least one fluid via; andat least one fluidic channel in fluidic communication with the at least one fluid via;wherein the microfluidic chip further comprises the pipette interface of any one of Claims 1-26 affixed to the first side of the microfluidic chip.Page 26 of 28FOLEYHO AGUS 12700946.3PFT-0042528. The microfluidic chip of Claim 27, wherein the fluid via includes a first opening disposed on the first side of the microfluidic chip, the second opening of the second inlet concentrically aligned with the first opening of the fluid via.

29. The microfluidic chip of Claim 27 or 28, wherein a surface of the microfluidic device includes a tapered groove configured to receive a portion of the core.Page 27 of 28FOLEYHO AGUS 12700946.3

Images