Skin composition and cosmetic method using the same

Abstract

Provided are skin compositions capable of sufficiently showing the efficacy of trisodium ascorbyl palmitate phosphate. The skin composition comprises at least 0.01% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition; 0.10 to 5.00% by weight of an ascorbyl phosphate salt based on the total weight of the skin composition; at least 0.005% by weight of polyquaternium-61 based on the total weight of the skin composition; 0.01 to 0.50% by weight of sodium hyaluronate based on the total weight of the skin composition; 2.50 to 7.50% by weight of polyols based on the total weight of the skin composition; and water, and has a pH in the range of 6.0 to 8.5.

Description

SKIN COMPOSITION AND COSMETIC METHOD USING THE SAME

[0001] The present invention relates to skin compositions comprising trisodium ascorbyl palmitate phosphate, an ascorbyl phosphate salt, polyquaternium-61, sodium hyaluronate, and polyols.

[0002] Trisodium ascorbyl palmitate phosphate, a derivative of ascorbic acid (vitamin C), is used in skincare products for its efficacy such as anti-aging because it promotes collagen production.

[0003] For example, commercially available DR.CI:LABO VC100 Essence Lotion EX (JNTL Consumer Health K.K.) is a Vitamin C product containing Water, dipropylene glycol (DPG), glycerin, 1,2-hexanediol, trisodium ascorbyl palmitate phosphate, 3-O-ethyl ascorbic acid, niacinamide, hydrolyzed collagen, soluble collagen crosspolymer, sodium hyaluronate, hydrolyzed hyaluronic acid, sodium acetylated hyaluronate, butylene glycol (BG), sodium pyrrolidone carboxylic acid (sodium PCA), PEG-10 methyl ether dimethicone, PEG-30 glyceryl cocoate, PEG-32, PEG-75, PPG-10 methyl glucose, PPG-20 methyl glucose, arginine, glycolic acid, trehalose, polyquaternium-51, polysorbate 80, menthyl lactate, sodium citrate, phenoxyethanol, grapefruit peel oil, and orange peel oil.

[0004] Commercially available DR.CI:LABO UV & White Moisture Milk SPF 50+ PA++++ (JNTL Consumer Health K.K.) is a sunscreen emulsion containing trisodium ascorbyl palmitate phosphate, sodium ascorbyl phosphate, along with Cyclopentasiloxane, water, zinc oxide, ethylhexyl methoxycinnamate, butylene glycol, polymethylsilsesquioxane, methyl methacrylate crosspolymer, ethylhexyl salicylate, phenylbenzimidazole sulfonic acid, silica, diethylamino hydroxybenzoyl hexyl benzoate, lauryl PEG-10 tris(trimethylsiloxy)silylethyl dimethicone, Verbascum thapsus extract, Vaccinium vitis-idaea fruit extract, cacao seed extract, Alteromonas ferment extract, Tabebuia impetiginosa bark extract, Mentha piperita (peppermint) leaf extract, hydroxyapatite, mica, Paeonia albiflora root extract, dimethicone / vinyl dimethicone crosspolymer, Cynara scolymus (artichoke) leaf extract, Rosa multiflora fruit extract, trisodium ascorbyl palmitate phosphate, resveratrol, Oenothera biennis seed extract, bilberry leaf extract, hydrolysed Prunus domestica, phenylethyl resorcinol, hydrolyzed collagen PG-propyl methylsilanediol, soluble collagen, hydrolyzed collagen, hydroxypropyl trimonium hyaluronate, sodium hyaluronate crosspolymer, sodium hyaluronate, ceramide NP, polyquaternium-51, dipotassium glycyrrhizate, tocopherol, glycerin, pentylene glycol, xanthan gum, dimethicone, hydrogen dimethicone, hydrated silica, calcium gluconate, gluconolactone, ethylhexyl triazone, bis-ethylhexyloxyphenol methoxyphenyl triazine, citric acid, sodium chloride, sodium hydroxide, polyglyceryl-2 triisostearate, laureth-4, and phenoxyethanol.

[0005] JP6209855JP7114897

[0006] As described above, trisodium ascorbyl palmitate phosphate has efficacy such as anti-aging. However, trisodium ascorbyl palmitate phosphate is instable. Therefore, skin compositions capable of sufficiently showing the efficacy of trisodium ascorbyl palmitate phosphate have not yet been provided.

[0007] In response to the above problem, an object of the present invention is to provide a skin composition capable of sufficiently showing the efficacy of trisodium ascorbyl palmitate phosphate. Another object of the present invention is to provide a skin composition capable of maintaining trisodium ascorbyl palmitate phosphate in a stable state. A further object of the present invention is to provide a cosmetic method for treating skin with the skin composition.

[0008] The inventors earnestly examined the problem in order to achieve the above-described objects, and concluded that adjusting the contents of trisodium ascorbyl palmitate phosphate, ascorbyl phosphate salt, polyquaternium-61, sodium hyaluronate, and polyols in the skin composition to their prescribed ranges allows trisodium ascorbyl palmitate phosphate to sufficiently show its efficacy and to be maintained in a stable state.

[0009] In view of the above conclusion, the present invention comprises the following Configurations: (Configuration 1) A skin composition comprising: at least 0.01% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition; 0.10 to 5.00% by weight of an ascorbyl phosphate salt based on the total weight of the skin composition; at least 0.005% by weight of polyquaternium-61 based on the total weight of the skin composition; 0.01 to 0.50% by weight of sodium hyaluronate based on the total weight of the skin composition; 2.50 to 7.50% by weight of polyols based on the total weight of the skin composition; and water, wherein the skin composition has a pH in the range of 6.0 to 8.5.

[0010] (Configuration 2) The skin composition of Configuration 1, comprising 0.01 to 1.00% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition.

[0011] (Configuration 3) The skin composition of Configuration 1 or 2, comprising 0.01 to 0.15% by weight of sodium hyaluronate based on the total weight of the skin composition.

[0012] (Configuration 4) The skin composition of any one of Configurations 1 to 3, wherein the ascorbyl phosphate salt is selected from the group consisting of sodium ascorbyl phosphate, magnesium ascorbyl phosphate and mixtures thereof.

[0013] (Configuration 5) The skin composition of any one of Configurations 1 to 4, wherein the polyols are selected from the group consisting of glycerin, butylene glycol, 1,2-hexanediol, and mixtures thereof.

[0014] (Configuration 6) The skin composition of any one of Configurations 1 to 5, in the form of a single-phase aqueous solution.

[0015] (Configuration 7) A skin composition comprising: 0.20 to 0.30% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition; 0.30 to 0.50% by weight of sodium ascorbyl phosphate based on the total weight of the skin composition; 0.010 to 0.020% by weight of polyquaternium-61 based on the total weight of the skin composition; 0.095 to 0.115% by weight of sodium hyaluronate based on the total weight of the skin composition; 4.00 to 5.00% by weight of polyols based on the total weight of the skin composition; and water, wherein the skin composition has a pH in the range of 6.0 to 8.5.

[0016] (Configuration 8) The skin composition of Configuration 7, in the form of a single-phase aqueous solution.

[0017] (Configuration 9) A cosmetic method comprising applying the skin composition of Configuration 1 or 7 on the entire face.

[0018] As described above, the skin composition according to the present invention comprises at least 0.01% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition; 0.10 to 5.00% by weight of an ascorbyl phosphate salt based on the total weight of the skin composition; at least 0.005% by weight of polyquaternium-61 based on the total weight of the skin composition; 0.01 to 0.50% by weight of sodium hyaluronate based on the total weight of the skin composition; 2.50 to 7.50% by weight of polyols based on the total weight of the skin composition; and water, and has a pH in the range of 6.0 to 8.5. Thus, the skin composition according to the present invention may provide excellent skin glow. Further, the skin composition comprising 0.01 to 1.00% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition may provide superior stability of trisodium ascorbyl palmitate phosphate.

[0019] Another skin composition according to the present invention comprises 0.20 to 0.30% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition; 0.30 to 0.50% by weight of sodium ascorbyl phosphate based on the total weight of the skin composition; 0.010 to 0.020% by weight of polyquaternium-61 based on the total weight of the skin composition; 0.095 to 0.115% by weight of sodium hyaluronate based on the total weight of the skin composition; 4.00 to 5.00% by weight of polyols based on the total weight of the skin composition; and water, and has a pH in the range of 6.0 to 8.5. Thus, this skin composition according to the present invention is expected to provide excellent skin glow, and superior stability of trisodium ascorbyl palmitate phosphate.

[0020] Fig. 1 shows example photographs showing skin of a subject prior to start of test and after eight weeks of application of the skin composition, respectively.Fig. 2 shows example photographs showing skin of a subject who received IPL treatment, prior to start of test and after a lapse of eight weeks, respectively.Fig. 3 shows example photographs showing H&E staining results.Fig. 4 shows example photographs showing immunofluorescence detection results of Collagen I.Fig. 5 shows example photographs showing immunofluorescence detection results of Collagen III.Fig. 6 shows example photographs showing immunofluorescence detection results of Collagen IV.Fig. 7 shows example photographs showing immunofluorescence detection results of Collagen VII.Fig. 8 shows example photographs showing immunofluorescence detection results of Collagen XVII.Fig. 9 shows example photographs showing masson staining results.Fig. 10 shows example photographs taken with a skin ultrasound instrument.

[0021] Unless defined otherwise, all technical and scientific terms used herein have the meaning commonly understood by one of ordinary skill in the art to which the invention pertains. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference.

[0022] As used herein, “applying” means directly laying on or spreading on outer skin, the scalp, or hair, e.g., by use of the hands or an applicator such as a wipe, roller, or spray.

[0023] As used herein, “cosmetically acceptable” means the ingredients the term describes are suitable for use in contact with tissues (e.g., the skin or hair) without undue toxicity, incompatibility, instability, irritation, allergic response, or the like.

[0024] As used herein, a “cosmetically acceptable active agent” is a compound (synthetic or natural) that has a cosmetic or therapeutic effect on the skin or hair.

[0025] Skin compositions of the present invention are suitable for treating signs of skin aging. As used herein, "signs of skin aging" includes the presence of lines including fine lines and wrinkles, loss of elasticity, uneven skin, blotchiness, diminished skin thickness, and abnormal or diminished synthesis of collagen, glycosaminoglycans, proteoglycans, elastin, or glycoproteins including fibronectin. In one embodiment, the sign of aging is selected from the presence of lines, fine lines, wrinkles, loss of elasticity, and abnormal or diminished synthesis of collagen, glycosaminoglycans, proteoglycans, elastin, or glycoproteins including fibronectin.

[0026] Skin compositions of the invention are also suitable for treating skin in need of brightening or lightening treatment. As used herein, the terms “brightening” or “lightening” the skin refers generally to lightening, brightening, whitening, and / or evening of the skin tone, skin color, and / or shade of skin, and / or to the reduction in sallowness, and / or to the lightening and / or fading of hyperpigmented marks and / or lesions including, but not limited to, pigmented spots, melanin spots, age spots, sun spots, senile lentigos, freckles, lentigos simplex, pigmented solar keratosis, seborrhoeic keratosis, melasma, acne marks, post-inflammatory hyperpigmentation, lentigines, ephelides, combinations of two or more thereof and the like. “Brightening” or “lightening” the skin also refers to increased skin radiance, glow, translucency and / or luminescence and / or obtaining a more radiant, glowing, translucent or luminous skin tone appearance or a less yellow or sallow skin tone. In certain preferred embodiments, “brightening” or “lightening” the skin refers to lightening and evening the skin tone, increasing skin radiance and / or lightening age spots. In certain other preferred embodiments, the present invention is directed to skin compositions and cosmetic methods for use on skin in need of skin brightening or lightening treatment selected from sallow and / or darkened skin. In certain other preferred embodiments, the present invention is directed to skin compositions and cosmetic methods for use on skin in need of skin brightening or lightening treatment selected from the group consisting of age spots, freckles, marks left after acne, and combinations of two or more thereof.

[0027] As used herein, “treatment” or “treating” means the amelioration, prophylaxis, or reversal of a condition, disease, or disorder, or at least one discernible symptom thereof. In one embodiment, “treatment” or “treating” refers to an amelioration, prophylaxis, or reversal of at least one measurable physical parameter related to the condition, disease, or disorder being treated, not necessarily discernible in or by the subject being treated. In another embodiment, “treatment” or “treating” refers to inhibiting or slowing the progression of a condition, disease, or disorder, either physically, e.g., stabilization of a discernible symptom, physiologically, e.g., stabilization of a physical parameter, or both. In another embodiment, “treatment” or “treating” refers to delaying the onset of a condition, disease, or disorder.

[0028] In certain embodiments, a skin composition of the invention is administered as a preventative measure. As used herein, “prevention” or “preventing” refers to a reduction of the risk of acquiring a given condition, disease, or disorder.

[0029] More broadly, the skin compositions of the invention may also be used to treat or prevent cosmetic, dermatological, or other conditions and disorders including, but not limited to, infections, deranged or disordered cutaneous or mucocutaneous tissue relevant to skin, nail and hair; oral, vaginal and anal mucosa; disturbed keratinization; inflammation; changes associated with intrinsic and extrinsic aging, and others which may or may not be related to cutaneous system. The manifestations include, but are not limited to, oily skin; acne; rosacea; age spots; blemished skin; blotches; cellulite; dermatoses; dermatitis; skin, nail and hair infections; dandruff; dryness or looseness of skin, nail and hair; xerosis; inflammation, or eczema; elastosis; herpes; hyperkeratosis; hyperpigmented skin; ichthyosis; keratoses; lentigines; melasmas; mottled skin; pseudofolliculitis barbae; photoaging and photodamage; pruritus; psoriasis; skin lines; stretch marks; thinning of skin, nail plate and hair; warts; wrinkles; oral or gum disease; irritated, inflamed, red, unhealthy, damaged or abnormal mucosa, skin, hair, nail, nostril, ear canal, anal or vaginal conditions; breakdown, defective synthesis or repair of dermal components; abnormal or diminished synthesis of collagen, glycosaminoglycans, proteoglycans and elastin, as well as diminished levels of such components in the dermis; uneven skin tone; uneven and rough surface of skin, nail and hair; loss or reduction of skin, nail and hair resiliency, elasticity and recoilability; laxity; lack of skin, nail and hair lubricants and luster; fragility and splitting of nail and hair; yellowing skin; reactive, irritating or telangiectatic skin; and dull and older-looking skin, nail and hair. In addition, the skin compositions of the current invention can be used for general care of skin, nail and hair; to improve skin texture and pores, flakiness and redness; to make skin soft, smooth, fresh, balanced, visibly clear, even-toned and brighter; to increase skin fullness and plumpness; and for skin bleach and lightening and wound healing; to reduce or prevent sweating or perspiration of underarm, crotch, palm, or other parts of the body.

[0030] As used herein, the term “subject” means any animal, preferably a mammal, most preferably a human, to whom a skin composition of the invention will be or has been administered. The term “mammal” as used herein, encompasses any mammal. Examples of mammals include, but are not limited to, cows, horses, sheep, pigs, cats, dogs, mice, rats, rabbits, guinea pigs, monkeys, and humans. In a preferred embodiment, the subject is a human.

[0031] As used herein, "wrinkle" includes fine lines, fine wrinkles, or coarse wrinkles. Examples of wrinkles include, but are not limited to, fine lines around the eyes (e.g., “crow’s feet”), forehead and cheek wrinkles, frown-lines, and laugh-lines around the mouth.

[0032] As used herein, "loss of elasticity" includes loss of elasticity or structural integrity of the skin or tissue, including but not limited to sagging, lax and loose tissue. The loss of elasticity or tissue structure integrity may be a result of a number of factors, including but not limited to disease, aging, hormonal changes, mechanical trauma, environmental damage, or the result of an application of products, such as a cosmetics or pharmaceuticals, to the tissue.

[0033] As used herein, "uneven skin" means a condition of the skin associated with diffuse or mottled pigmentation, which may be classified as hyperpigmentation, such as post-inflammatory hyperpigmentation.

[0034] As used herein, "blotchiness" means a condition of the skin associated with redness or erythema.

[0035] As used herein, "cosmetic" refers to beautifying or preserving, restoring, bestowing, simulating, or enhancing the appearance of bodily beauty or appearing to enhance beauty or youthfulness, specifically as it relates to the appearance of tissue or skin.

[0036] As used herein, "cosmetically effective amount" means an amount sufficient for treating or preventing one or more signs of skin aging, but low enough to avoid serious side effects. The cosmetically effective amount of the compound or composition will vary with the particular condition being treated, the age and physical condition of the end user, the severity of the condition being treated / prevented, the duration of the treatment, the nature of other treatments, the specific compound or product / composition employed, the particular cosmetically acceptable carrier utilized, and like factors.

[0037] As used herein, “substantially free of” means the ingredient referred to is not directly or intentionally added to the formula. Preferably, “substantially free of” means containing less than about 1% of an ingredient. More preferably, “substantially free of” means containing less than about 0.5% of an ingredient. Even more preferably, “substantially free of” means containing less than about 0.1% by weight of an ingredient. The present compositions may be completely free of an ingredient, i.e., contain none of the ingredient.

[0038] Unless otherwise indicated, a percentage or concentration refers to a percentage or concentration by weight (i.e., % (W / W)). A weight percent of an ingredient is based on the total weight of the composition containing the ingredient. Unless stated otherwise, all ranges are inclusive of the endpoints, e.g., “from 4 to 9” includes the endpoints 4 and 9.

[0039] Trisodium Ascorbyl Palmitate Phosphate The skin composition of the invention comprises trisodium ascorbyl palmitate phosphate.

[0040] The skin composition of the invention contains at least 0.01% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition. The content of trisodium ascorbyl palmitate phosphate of at least 0.01% by weight allows trisodium ascorbyl palmitate phosphate to show its efficacy. Preferably, the skin composition of the invention contains 0.01 to 2.00% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition. More preferably, the skin composition of the invention contains 0.01 to 1.00% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition. The content of trisodium ascorbyl palmitate phosphate of 1.00% by weight or less allows trisodium ascorbyl palmitate phosphate to be maintained in a stable state. Even more preferably, the skin composition of the invention contains 0.10 to 1.00% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition. The content of trisodium ascorbyl palmitate phosphate in the range of 0.10 to 1.00% by weight allows trisodium ascorbyl palmitate phosphate to sufficiently show its efficacy and to be maintained in a stable state. Further preferably, the skin composition of the invention contains 0.10 to 0.50% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition. Still further preferably, the skin composition of the invention contains 0.20 to 0.30% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition.

[0041] Trisodium ascorbyl palmitate phosphate is commercially available, for example, as Apprecier from Resonac Corporation.

[0042] Ascorbyl Phosphate Salt The skin composition of the invention contains an ascorbyl phosphate salt. The ascorbyl phosphate salt may be selected, for example, from the group consisting of sodium ascorbyl phosphate and magnesium ascorbyl phosphate.

[0043] The ascorbyl phosphate salt may be sodium ascorbyl phosphate.

[0044] The skin composition of the invention contains 0.10 to 5.00% by weight of ascorbyl phosphate salt based on the total weight of the skin composition. The content of ascorbyl phosphate salt in the range of 0.10 to 5.00% by weight allows ascorbyl phosphate salt to show its skin-whitening effect. Preferably, the skin composition of the invention contains 0.10 to 3.00% by weight of ascorbyl phosphate salt based on the total weight of the skin composition. More preferably, the skin composition of the invention contains 0.30 to 0.50% by weight of ascorbyl phosphate salt based on the total weight of the skin composition.

[0045] Sodium ascorbyl phosphate is commercially available, for example, as Ascorbyl PS (APS) from Resonac Corporation, and StayC-50 from DSM. Magnesium ascorbyl phosphate is commercially available, for example, as Ascorbyl PM (APM) from Resonac Corporation.

[0046] Polyquaternium-61 The skin composition of the invention also contains polyquaternium-61.

[0047] The skin composition of the invention contains at least 0.005% by weight of polyquaternium-61 based on the total weight of the skin composition. The content of polyquaternium-61 of at least 0.005% by weight may improve the stability of trisodium ascorbyl palmitate phosphate. Preferably, the skin composition of the invention contains 0.005 to 0.500% by weight of polyquaternium-61 based on the total weight of the skin composition. The content of polyquaternium-61 in the range of 0.005 to 0.500% by weight may improve the stability of trisodium ascorbyl palmitate phosphate, but does not block the efficacy of trisodium ascorbyl palmitate phosphate. More preferably, the skin composition of the invention contains 0.005 to 0.050% by weight of polyquaternium-61 based on the total weight of the skin composition. Preferably, the skin composition of the invention contains 0.010 to 0.020% by weight of polyquaternium-61 based on the total weight of the skin composition.

[0048] Polyquaternium-61 is commercially available, for example, as Lipidure NR or Lipidure S from NOF corporation.

[0049] Sodium Hyaluronate The skin composition of the invention also contains sodium hyaluronate.

[0050] The skin composition of the invention contains 0.01 to 0.50% by weight of sodium hyaluronate based on the total weight of the skin composition. The content of sodium hyaluronate in the range of 0.01 to 0.50% by weight allows sodium hyaluronate to show its penetration effect. Preferably, the skin composition of the invention contains 0.01 to 0.20% by weight of sodium hyaluronate based on the total weight of the skin composition. More preferably, the skin composition of the invention contains 0.01 to 0.15% by weight of sodium hyaluronate based on the total weight of the skin composition. Further preferably, the skin composition of the invention contains 0.095 to 0.115% by weight of sodium hyaluronate based on the total weight of the skin composition.

[0051] Sodium hyaluronate is commercially available, for example, as Bio-Sodium Hyaluronate 1% Solution (J Type) from Hyundai Bioland Co., Ltd.

[0052] Polyols The skin composition of the invention also contains polyols. The polyols are preferably selected from the group consisting of glycerin, butylene glycol, 1,2-hexane diol, propanediol, pentylene glycol, and mixtures thereof.

[0053] The skin composition of the invention contains 2.50 to 7.50% by weight of polyols based on the total weight of the skin composition. The content of polyols in the range of 2.50 to 7.50% by weight does not block the efficacy of trisodium ascorbyl palmitate phosphate. Preferably, the skin composition of the invention contains 4.00 to 5.00% by weight of polyols based on the total weight of the skin composition.

[0054] Glycerin is commercially available, for example, as Cosmetic Grade Concentrated Glycerin from Sakamoto Yakuhin Kogyo co., ltd., as RG・CO・P from NOF corporation, and as GLYCERINE (COSMETIC GRADE) from Kao Corporation. Butylene glycol is commercially available, for example, as 1,3-BG from Daicel Corporation. 1,2-hexane diol is commercially available, for example, as LexGard H from INOLEX, Inc. and as effisin HD from Ashland Japan. Propanediol is commercially available, for example, as effisin Propanediol from Ashland Japan. Pentylene glycol is commercially available, for example, as DIOL PD from KOKYU ALCOHOL KOGYO CO., LTD.

[0055] Water-Containing Vehicle The skin composition of the invention also comprises water. Preferably, the skin composition of the invention comprises at least 60% by weight of water based on the total weight of the skin composition. The skin composition of the invention may comprise 70% by weight of water based on the total weight of the skin composition.

[0056] The skin composition of the invention may be in the form of a single-phase aqueous solution. In such case, it comprises at least 70% by weight of water based on the total weight of the skin composition. For example, the skin composition may comprise at least 75% by weight of water based on the total weight of the skin composition. The skin composition in the form of a single-phase aqueous solution is light and easily spread on and absorbed by the skin and can therefore be used as a skin lotion.

[0057] The skin composition may be in the form of an oil-in-water emulsion. In such case, it comprises at least 60% by weight of water based on the total weight of the skin composition. For example, the skin composition may comprise at least 62% by weight of water based on the total weight of the skin composition. The skin composition in the form of an oil-in-water emulsion creates a barrier with its oil content to prevent evaporation of moisture supplied to skin by, for example, a skin lotion and to keep skin moist, and thus, can be used as a milky lotion.

[0058] Other Cosmetically Acceptable Active Agents The skin composition of the invention may contain one or more other cosmetically acceptable active agents.

[0059] Cosmetically acceptable active agents include for example other anti-aging agents, other antioxidants, anti-acne agents, shine control agents, anti-microbial agents, anti-inflammatory agents, anti-mycotic agents, anti-parasite agents, external analgesics, sunscreens, photoprotectors, keratolytic agents, surfactants, moisturizers, nutrients, other vitamins, energy enhancers, anti-perspiration agents, astringents, deodorants, firming agents, anti-callous agents, and agents for hair and / or skin conditioning.

[0060] The amount of other cosmetically acceptable active agent in the skin composition may range from about 0.001% to about 20% by weight, e.g., about 0.005% to about 10% by weight, such as about 0.01% to about 5% by weight, based on the total weight of the skin composition.

[0061] The cosmetically acceptable active agent may be selected for instance from alpha and polyhydroxy acids such as glycolic acid, lactic acid, malic acid, salicylic acid, citric acid, tartaric acid, and gluconolactone, benzoyl peroxide, D-panthenol carotenoids, retinoids such as retinol and retinyl palmitate, ceramides, polyunsaturated fatty acids, essential fatty acids, enzymes such as laccase, enzyme inhibitors, minerals, hormones such as estrogens, steroids such as hydrocortisone, 2-dimethylaminoethanol, copper salts such as copper chloride, peptides such as dipeptides including N-acyl dipeptide derivatives including Acetyl Dipeptide-31 Amide, tripeptides, argireline and syn-ake, those containing copper, coenzyme Q10, amino acids such as proline, other vitamins, lactobionic acid, acetyl-coenzyme A, niacin, riboflavin, thiamin, ribose, electron transporters such as NADH and FADH2, natural extracts such as those from Moringa Oleifera seed, aloe vera, feverfew (for example, Chrysanthemum Parthenium (Feverfew) Flower / Leaf / Stem Juice), oatmeal, dill, blackberry, princess tree, lemon aspen, resorcinols such as 4-hexyl resorcinol, curcuminoids, sugar amines such as N-acetyl glucosamine, glycosaminoglycans such as hyaluronic acid and chondroitin sulfate, niacinamide, trehalose and derivatives and mixtures thereof.

[0062] Examples of other vitamins include, but are not limited to, vitamin A, vitamin B’s such as vitamin B3, vitamin B5, and vitamin B12, vitamin K, and different forms of vitamin E such as alpha, beta, gamma or delta tocopherols or their mixtures, and derivatives thereof.

[0063] Examples of other antioxidants include, but are not limited to, water-soluble antioxidants such as sulfhydryl compounds and their derivatives (e.g., N-acyl-cysteine), lipoic acid and dihydrolipoic acid, resveratrol, lactoferrin, and other ascorbic acid derivatives (e.g., ethyl L-ascorbic acid, ascorbyl palmitate and ascorbyl polypeptide) and epigallocatechin gallate (EGCG). Oil-soluble antioxidants suitable for use in the skin compositions of this invention include, but are not limited to, butylated hydroxytoluene, retinoids (e.g., retinol and retinyl palmitate), tocopherols (e.g., tocopherol acetate), tocotrienols, and ubiquinone. Natural extracts containing antioxidants suitable for use in the skin compositions of this invention, include, but not limited to, extracts containing flavonoids and isoflavonoids and their derivatives (e.g., genistein and diadzein), extracts containing resveratrol and the like. Examples of such natural extracts include grape seed, pine bark, and propolis.

[0064] Preferably, the skin composition of the invention comprises another cosmetically acceptable active ingredient selected from niacinamide, trehalose, hydrolyzed collagen, and hydrolyzed hyaluronic acid.

[0065] The skin composition of the invention may contain one or more emollients as known in the art. As used herein, "emollients" refer to materials used for the prevention or relief of dryness, such as by preventing the transepidermal loss of water from the skin. Examples of emollients include, but are not limited to, those set forth in the International Cosmetic Ingredient Dictionary and Handbook, eds. Pepe, Wenninger and McEwen, pp. 2930-36 (The Cosmetic, Toiletry, and Fragrance Assoc., Washington, D.C., 9th Edition, 2002) (hereinafter “ICI Handbook”). Examples of particularly suitable emollients include vegetable oils, mineral oils, fatty esters, and the like.

[0066] The skin composition of the invention may contain, in addition to the above components, a wide variety of additional oil-soluble materials and / or water-soluble materials conventionally used in compositions for use on skin and hair, at their art-established levels. These include humectants, pH adjusters, fragrances, dyes, and preservatives (e.g., parabens).

[0067] The skin composition of the invention may comprise one or more oils. As used herein, the term “oil” means a hydrophobic material, for example hydrocarbon-based oils, silicones, fatty acid derivatives, glycerides, vegetable oils, vegetable oil derivatives, alkyl esters, wax esters, beeswax derivatives, sterols, and phospholipids.

[0068] Suitable hydrocarbon oils include petrolatum, mineral oil, micro-crystalline waxes, squalene and combinations thereof.

[0069] Suitable silicone oils include dimethicone, dimethiconol, phenyl dimethicone and cyclic polysiloxanes and combinations thereof. Silicone oils having viscosities from about 0.5 to about 100,000 centistokes at 25° C may also be useful in the skin composition of the invention.

[0070] Suitable glycerides include castor oil, sunflower seed oil, coconut oil and derivatives, vegetable oils and derivatives, palm oil, jojoba oil, Shea butter, lanolin and combinations thereof.

[0071] Suitable alkyl ester oils include, but are not limited to, isopropyl esters of fatty acids and esters of long chain fatty acids. More preferably, alkyl esters selected from the group consisting of isopropyl palmitate, isopropyl myristate, myristyl myristate, isohexyl palmitate, decyl oleate, isononyl isononanoate and combinations thereof are useful.

[0072] pH The skin composition of the invention has a pH in the range of 6.0 to 8.5. A pH in the range of 6.0 to 8.5 allows a vitamin C derivative to be maintained in a stable state. Preferably, the skin composition of the invention has a pH in the range of 7.0 to 8.5.

[0073] Method of Preparing Skin Composition The skin compositions of the present invention may be prepared using methodology that is well known in the art.

[0074] Methods of Treating Skin The skin composition of the invention is applied to skin. The skin may be treated by applying the skin composition of the invention.

[0075] Effects The skin composition of the invention comprises at least 0.01% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition; 0.10 to 5.00% by weight of an ascorbyl phosphate salt based on the total weight of the skin composition; at least 0.005% by weight of polyquaternium-61 based on the total weight of the skin composition; 0.01 to 0.50% by weight of sodium hyaluronate based on the total weight of the skin composition; 2.50 to 7.50% by weight of polyols based on the total weight of the skin composition; and water, and has a pH in the range of 6.0 to 8.5. Thus, the skin composition of the invention may provide excellent skin glow, and penetrates the skin well. Further, the skin composition comprising 0.01 to 1.00% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition may provide superior stability of trisodium ascorbyl palmitate phosphate. In addition, the skin composition comprising 0.01 to 0.15% by weight of sodium hyaluronate based on the total weight of the skin composition is non-sticky.

[0076] Another skin composition of the invention comprises 0.20 to 0.30% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition; 0.30 to 0.50% by weight of sodium ascorbyl phosphate based on the total weight of the skin composition; 0.010 to 0.020% by weight of polyquaternium-61 based on the total weight of the skin composition; 0.095 to 0.115% by weight of sodium hyaluronate based on the total weight of the skin composition; 4.00 to 5.00% by weight of polyols based on the total weight of the skin composition; and water, and has a pH in the range of 6.0 to 8.5. Thus, this skin composition of the invention is expected to provide excellent skin glow, and superior stability of trisodium ascorbyl palmitate phosphate.

[0077] The following non-limiting examples further illustrate the invention.

[0078] Examples 1 to 4 Skin compositions of Examples 1 to 4 and Comparative Examples 1 to 7 were made using ingredients in Table 1. The skin compositions of Examples 1 to 4 were in the form of single-phase aqueous solutions. The skin compositions of Examples 1 to 4 and Comparative Examples 1 to 6 had a pH in the range of 6.0 to 8.5. The skin composition of Comparative Example 7 had a pH outside the range of 6.0 to 8.5.

[0079] The skin compositions of Examples 1 to 4 and Comparative Examples 1 to 7 were prepared as follows. All ingredients were added to water at room temperature. The resulting mixture was then stirred to homogenate it.

[0080] The skin compositions of Examples 1 to 4 and Comparative Examples 1 to 7 were evaluated using the following four tests. Table 1 shows the results of the tests.

[0081] 1. Skin Glow (“Skin Glow Test”): Six human subjects applied a usual usage amount of a skin composition on their entire faces. After application, they evaluated whether or not they felt the skin composition improved their skin glow. The results were compiled, and the skin composition was assigned one of the following grades: AA: 100% agreed they had improvement in skin glow A: More than 67% agreed they had improvement in skin glow (4 or 5 out of 6) B: More than 33% agreed they had improvement in skin glow (2 or 3 out of 6) C: Less than 33% agreed they had improvement in skin glow (1 or 0 out of 6).

[0082] 2. Stability (“Stability Test”): A skin composition was prepared in a glass bottle and stored in a 50° C chamber for 7 days. The skin composition was then evaluated visually with the unaided human eye at a distance of approximately 30.48 cm (approximately 12 inches) and assigned one of the following scores: AA: Transparent with no color A: Transparent and slightly yellow B: Slightly cloudy and slightly yellow C: Precipitates observed and yellow.

[0083] 3. Stickiness (“Stickiness Test”): Six human subjects applied a usual usage amount of a skin composition on their entire faces. After application, they evaluated the skin composition as “sticky” or “not sticky.” The results were compiled, and the skin composition was assigned one of the following grades: AA: 100% agree not sticky A: More than 67% agree not sticky (4 or 5 out of 6) B: More than 33% agree not sticky (2 or 3 out of 6) C: Less than 33% agree not sticky (1 or 0 out of 6).

[0084] 4. Penetration (“Penetration Test”): Six human subjects applied a usual usage amount of a skin composition on their entire faces. After application, they evaluated whether or not they felt the skin composition penetrated their skin. The results were compiled, and the skin composition was assigned one of the following grades: AA: 100% felt penetration A: More than 67% felt penetration (4 or 5 out of 6) B: More than 33% felt penetration (2 or 3 out of 6) C: Less than 33% felt penetration (1 or 0 out of 6).

[0085] Table 1 shows the results of the tests for the skin compositions of Examples 1 to 4 and Comparative Examples 1 to 7.

[0086]

[0087] <Skin Glow> The skin compositions of Examples 1 to 4 were rated "A" or better in Skin Glow Test. This is probably because the concentrations of the ingredients, i.e., trisodium ascorbyl palmitate phosphate, sodium ascorbyl phosphate, polyquaternium-61, sodium hyaluronate, and polyols, were within their respective appropriate ranges and the pH was also within the appropriate range. On the other hand, the skin compositions of Comparative Examples 1 and 2 were rated "B" in Skin Glow Test. This is probably because the skin composition of Comparative Example 1 did not contain 1, 2-hexanediol and had a low total polyol content, which decreased the stability of trisodium ascorbyl palmitate phosphate, and consequently few human subjects felt improvement in skin glow, and probably because the skin composition of Comparative Example 2 had a high total polyol content, which also decreased the stability of trisodium ascorbyl palmitate phosphate, and consequently few human subjects felt improvement in skin glow. Based on the results of Comparative Examples 1 and 2, there should be an appropriate range for the total polyol content. The skin composition of Comparative Example 3 was rated "B" in Skin Glow Test. This is probably because it had a low content of polyquaternium-61, which decreased the stability of trisodium ascorbyl palmitate phosphate, and consequently few human subjects felt improvement in skin glow. The skin composition of Comparative Example 4 was rated "C" in Skin Glow Test. This is probably because an absence of sodium hyaluronate resulted in low moisture-retaining properties and insufficient efficacy of trisodium ascorbyl palmitate phosphate, and consequently few human subjects felt improvement in skin glow. The skin composition of Comparative Example 5 was rated "C" in Skin Glow Test. This is probably because it failed to provide the efficacy of trisodium ascorbyl palmitate phosphate due to an absence of trisodium ascorbyl palmitate phosphate, and consequently few human subjects felt improvement in skin glow. The skin composition of Comparative Example 6 was rated "B" in Skin Glow Test. This is probably because it failed to provide the efficacy of sodium ascorbyl phosphate due to an absence of sodium ascorbyl phosphate, and consequently few human subjects felt improvement in skin glow. The skin composition of Comparative Example 7 was rated "C" in Skin Glow Test. This is probably because its pH was outside the appropriate range, which decreased the stability of trisodium ascorbyl palmitate phosphate, and consequently few human subjects felt improvement in skin glow.

[0088] <Stability> The skin compositions of Examples 1 to 3 were rated "A" or better in Stability Test. On the other hand, the skin composition of Example 4 showed a decrease in transparency and yellowed during Stability Test, and was rated "B" in Stability Test. This is probably because it had a high content of trisodium ascorbyl palmitate phosphate. However, it was rated "A" in Skin Glow Test, and it may improve skin glow. The skin compositions of Comparative Examples 1 and 2 were rated "C" and "B", respectively, in Stability Test. This is probably because the total polyol contents in the skin compositions of Comparative Examples 1 and 2 were outside the appropriate range. The skin composition of Comparative Example 3 was rated "B" in Stability Test. This is probably because it had a low content of polyquaternium-61. The skin composition of Comparative Example 7 was rated "C" in Stability Test. This is probably because its pH was outside the appropriate range.

[0089] <Stickiness> The skin compositions of Example 1, 2, and 4 were rated "A" or better in Stickiness Test. On the other hand, the skin composition of Example 3 was rated "C" in Stickiness Test. This is probably because it had a high content of sodium hyaluronate acting as a thickener. However, it was rated "A" in both Skin Glow Test and Stability Test, so it may improve skin glow and maintain the stability. The skin composition of Comparative Example 2 was rated "C" in Stickiness Test. This is probably because it had a high total content of polyols with adhesive properties. The skin composition of Comparative Example 4 was rated "C" in Stickiness Test. This is probably because it emphasized the stickiness of polyols due to an absence of sodium hyaluronate. The skin composition of Comparative Example 7 was rated "C" in Stickiness Test. This is probably because its pH was outside the range sufficient to show efficacy of the skin composition.

[0090] <Penetration> The skin compositions of Examples 1 to 4 were rated "A" or better in Penetration Test. On the other hand, the skin composition of Comparative Example 2 was rated "C" in Penetration Test. This is probably because it was highly sticky due to the high total polyol content, and consequently few human subjects felt penetration. The skin composition of Comparative Example 3 was rated "B" in Penetration Test. This is probably because it had a low content of polyquaternium-61, which decreased the stability, and thus the absorption, of trisodium ascorbyl palmitate phosphate. The skin composition of Comparative Example 4 was rated "C" in Penetration Test. This is probably because it did not contain sodium hyaluronate, and consequently few human subjects felt penetration. The skin composition of Comparative Example 7 was rated "B" in Penetration Test. This is probably because its pH was outside the range sufficient to show efficacy of the skin composition.

[0091] The skin compositions of Examples 1 and 2 were rated "A" or better in all the aforementioned four tests. From this, the skin compositions of Examples 1 and 2 were found to be non-sticky, penetrate the skin well, maintain trisodium ascorbyl palmitate phosphate in a stable state, and provide excellent skin glow.

[0092] Example 5 A skin composition of Example 5 was prepared in a manner similar to Examples 1 to 4 using the ingredients shown in Table 2. The skin composition of Example 5 is in the form of a single-phase aqueous solution, and used as a skin lotion. The skin composition of Example 5 had a pH in the range of 6.0 to 8.5. The skin composition of Example 5 had excellent stability and transparency.

[0093]

[0094] The skin composition of Example 5 was evaluated using the following tests.

[0095] 5. Blind Test Sixty-five human subjects applied a usual usage amount of the skin composition of Example 5 on their entire faces. This treatment was continued for four weeks. After four weeks of treatment, 74% of the human subjects felt improvement in skin glow, 88% felt improvement in skin plumpness, and 75% felt improvement in skin smoothness.

[0096] The results of Blind Test show that the skin composition of Example 5 may provide excellent skin glow, excellent skin plumpness, and excellent skin smoothness.

[0097] 6. Test for comparison with IPL Treatment Thirty Japanese female subjects between the ages of 31 and 45 applied a usual usage amount of the skin composition of Example 5 on their entire faces. The skin composition was applied twice a day, morning and evening, for eight weeks. Skin glow, stratum corneum moisture content, and skin elasticity were measured before starting the test and after eight weeks of application. After eight weeks of application in the 30 subjects, skin glow was improved by 8.7% on average, stratum corneum moisture content was improved by 21.6% on average, and skin elasticity was improved by 14.6% on average. Fig. 1 shows example photographs showing skin of a subject prior to start of test and after application of the skin composition of Example 5 under the above-described condition for eight weeks, respectively. Fig. 1 shows color photographs in grayscale. Fig. 1(A) shows the skin condition prior to start of test, and Fig. 1(B) shows the skin condition after eight weeks of application. The skin glow value was 5.22 prior to start of test and 6.48 after eight weeks of application, and the skin glow was improved by 24%.

[0098] On the other hand, twelve Japanese female subjects between the ages of 33 and 45 applied to the left sides of their faces skin compositions that they normally used. The skin compositions were applied twice a day, morning and evening, for eight weeks. During this period of time, the subjects received Intense Pulsed Light (IPS) treatment three times. Skin glow, stratum corneum moisture content, and skin elasticity were measured before starting the test and after eight weeks of treatment. After eight weeks of treatment in the 12 subjects, skin glow was improved by 6.9% on average, stratum corneum moisture content was improved by 20.8% on average, and skin elasticity was improved by 10.9% on average. Fig. 2 shows example photographs showing skin of a subject who received IPL treatment under the above-described condition, prior to start of test and after a lapse of eight weeks, respectively. Fig. 2 shows color photographs in grayscale. Fig. 2(A) shows the skin condition prior to start of test, and Fig. 2(B) shows the skin condition after eight weeks of treatment. The skin glow value was 5.11 prior to start of test and 5.51 after eight weeks of treatment, and the skin glow was improved by 7.9%.

[0099] Based on the result of Test for comparison with IPL treatment, the skin composition of Example 5 was found to provide excellent skin glow, excellent skin hydration, and excellent skin elasticity, equivalent to IPL treatment.

[0100] An apparatus used for IPL treatment is PHOTOSILK plus (DEKA M.E.L.A s.r.l.; Italy). IPL treatment was performed as follows. The subjects received the initial IPL treatment after baseline measurement. Then, after an interval of at least two weeks, the subjects received a total of three IPL treatments. The subjects completed the three IPL treatments by two weeks prior to the measurement in the eighth week. IPL output was 10J to 13J (The melisma area and under the nose may be treated with lower power). Treatment area was about 100 counts for 2 circles around the entire face (depending on the size of the face).

[0101] An apparatus used to measure skin glow is Glossymeter GL200 (Courage+Khazaka electronic GmbH; Germany). Skin glow is measured by analyzing reflection of light on skin surface. Five measurement points were set on one cheek, and five measurements was taken at each point. The average of these measurements was calculated, and the average value of the left and right sides was used for evaluation.

[0102] An apparatus used to measure stratum corneum moisture content is Corneometer (Courage+Khazaka electronic GmbH; Germany). Skin moisture content was measured by capacitance measurement. Measurable capacitance is mainly due to the moisture in the stratum corneum. Measurements were taken by shifting the apparatus slightly by about 5 mm sequentially around the cheekbone, ten measurements were taken on right and left sides, respectively, and the average value was used for evaluation.

[0103] An apparatus used to measure skin elasticity is Cutometer (Courage+Khazaka electronic GmbH; Germany). Skin elasticity was evaluated by suctioning the skin and measuring its deformation and return. Measurements were taken five times each on the left and right sides while shifting the apparatus approximately 10 mm around the center of the cheek, and the average value was used for evaluation.

[0104] 7. Ex-vivo Test A negative control group and a sample group were prepared as follows. In the negative control group, excised skin tissue was placed in a medium for two days and then irradiated with UV (30 J / cm2UVA and 50 mJ / cm2UVB) for four days. After UV irradiation, the medium was refreshed. After four consecutive days of UV irradiation, the skin tissue culture was continued for three days without UV irradiation. Throughout the 7-day culture period, the medium was refreshed daily. In the sample group, excised skin tissue was placed in a medium for two days and then irradiated with UV (30 J / cm2UVA and 50 mJ / cm2UVB) for four days. After UV irradiation, the medium was refreshed. Then, the skin composition of Example 5 was applied to the skin tissue. After four consecutive days of UV irradiation and the application of the skin composition of Example 5, the skin composition of Example 5 was applied without UV irradiation for three days to continue the skin tissue culture. Throughout the 7-day culture period, the medium was refreshed daily. The amount of the skin composition of Example 5 was 2 mg / cm2per application.

[0105] For the negative control and sample groups, the thickness of epidermal layer; the contents of Collagen I, Collagen III, Collagen IV, Collagen VII, and Collagen XVII; the content of collagen fiber; and collagen fiber density were measured.

[0106] Three skin tissues were used in measurement for each of the negative control and sample groups. Each skin tissue was measured at three locations. Thus, each of the negative control and sample groups was measured at nine locations. The average of the results of measurements at the nine locations was used to calculate the improvement rate described below.

[0107] The thickness of epidermal layer was measured using H&E staining. Fig. 3 shows example photographs showing H&E staining results. Fig. 3 shows color photographs in grayscale. Fig. 3(A) shows the negative control group, and Fig. 3(B) shows the sample group. The photographs were taken with an upright microscope (Olympus, BX53). Scale bar is 100 μm. The thickness of epidermal layer was measured with IPP 6.0 software, and the measurement unit was μm.

[0108] The contents of Collagen I, Collagen III, Collagen IV, Collagen VII, and Collagen XVII were measured by an immunofluorescence test.

[0109] Fig. 4 shows example photographs showing immunofluorescence detection results of Collagen I. Fig. 4 shows color photographs in grayscale. Fig. 4(A) shows the negative control group, and Fig. 4(B) shows the sample group. Scale bar in Fig. 4 is 50 μm. The green fluorescence in the color photographs is Collagen I. The stronger the green fluorescence intensity, the higher the content of Collagen I. The content of Collagen I was counted with IPP 6.0 software, and the statistical value was integrated optical density (IOD).

[0110] Fig. 5 shows example photographs showing immunofluorescence detection results of Collagen III. Fig. 5 shows color photographs in grayscale. Fig. 5(A) shows the negative control group, and Fig. 5(B) shows the sample group. Scale bar in Fig. 5 is 50 μm. The green fluorescence is Collagen III. The stronger the green fluorescence intensity, the higher the content of Collagen III. The content of Collagen III was counted with IPP 6.0 software, and the statistical value was integrated optical density (IOD).

[0111] Fig. 6 shows example photographs showing immunofluorescence detection results of Collagen IV. Fig. 6 shows color photographs in grayscale. Fig. 6(A) shows the negative control group, and Fig. 6(B) shows the sample group. Scale bar in Fig. 6 is 50 μm. The green fluorescence in the color photographs is Collagen IV. The stronger the green fluorescence intensity, the higher the content of Collagen IV. The content of Collagen IV was counted with IPP 6.0 software, and the statistical value was integrated optical density (IOD).

[0112] Fig. 7 shows example photographs showing immunofluorescence detection results of Collagen VII. Fig. 7 shows color photographs in grayscale. Fig. 7(A) shows the negative control group, and Fig. 7(B) shows the sample group. Scale bar in Fig. 7 is 50 μm. The green fluorescence in the color photographs is Collagen VII. The stronger the green fluorescence intensity, the higher the content of Collagen VII. The content of Collagen VII was counted with IPP 6.0 software, and the statistical value was integrated optical density / area (IOD / area).

[0113] Fig. 8 shows example photographs showing immunofluorescence detection results of Collagen XVII. Fig. 8 shows color photographs in grayscale. Fig. 8(A) shows the negative control group, and Fig. 8(B) shows the sample group. Scale bar in Fig. 8 is 50 μm. The green fluorescence in the color photographs is Collagen XVII. The stronger the green fluorescence intensity, the higher the content of Collagen XVII. The content of Collagen XVII was counted with IPP 6.0 software, and the statistical value was integrated optical density / area (IOD / area).

[0114] The content of collagen fiber was measured using masson staining. Fig. 9 shows example photographs showing masson staining results. Fig. 9 shows color photographs in grayscale. Fig. 9(A) shows the negative control group, and Fig. 9(B) shows the sample group. The photographs were taken with an upright microscope (Olympus, BX53). Scale bar is 100 μm. The blue color in the color photographs represents collagen fiber. The more blue, the higher the content of collagen fiber. The content of collagen fiber was counted with IPP 6.0 software, and the statistical value was relative area.

[0115] Collagen fiber density was measured using a skin ultrasound instrument (Cortex Texhnology, DermaLab Combo). Fig. 10 shows example photographs taken with the skin ultrasound instrument. Fig. 10 shows color photographs in grayscale. Fig. 10(A) shows the negative control group, and Fig. 10(B) shows the sample group. The dermis green (low density area) and yellow white (high density area) in the color photographs represent collagen. The more yellow and white, the brighter, and the higher the collagen density. Collagen fiber density was counted with IPP 6.0 software, and the statistical value was average density.

[0116] Improvement rate in the sample group relative to the negative control group was calculated using the following equation: Improvement rate (%) = ((sample group - negative control group) / negative control group) x 100

[0117] Based on the result of Ex-vivo Test, compared with the negative control group, the contents of Collagen IV, Collagen VII, and Collagen XVII in the sample group increased significantly, with the improvement rates of 100.00%, 124.39%, and 160.00%, respectively. Increase in the contents of Collagen IV, Collagen VII and Collagen XVII indicates that the skin composition of Example 5 may provide excellent skin tightening efficacy.

[0118] Further, based on the result of Ex-vivo Test, compared with the negative control group, the thickness of the epidermal layer; the contents of Collagen I, Collagen III, and collagen fiber; and collagen fiber density in the sample group increased significantly, with the improvement rates of 68.29%, 121.43%, 120.75%, 141.46%, and 35.42%, respectively. Improvement of tissue morphology; increase in the contents of Collagen I, Collagen III, and collagen fiber; and increase in collagen fiber density indicate that the skin composition of Example 5 may provide the anti-wrinkle efficacy.

Claims

1. A skin composition comprising: at least 0.01% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition; 0.10 to 5.00% by weight of an ascorbyl phosphate salt based on the total weight of the skin composition; at least 0.005% by weight of polyquaternium-61 based on the total weight of the skin composition; 0.01 to 0.50% by weight of sodium hyaluronate based on the total weight of the skin composition; 2.50 to 7.50% by weight of polyols based on the total weight of the skin composition; and water, wherein the skin composition has a pH in the range of 6.0 to 8.5.

2. The skin composition of claim 1, comprising 0.01 to 1.00% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition.

3. The skin composition of claim 1 or 2, comprising 0.01 to 0.15% by weight of sodium hyaluronate based on the total weight of the skin composition.

4. The skin composition of claim 1 or 2, wherein the ascorbyl phosphate salt is selected from the group consisting of sodium ascorbyl phosphate, magnesium ascorbyl phosphate and mixtures thereof.

5. The skin composition of claim 1 or 2, wherein the polyols are selected from the group consisting of glycerin, butylene glycol, 1,2-hexanediol, and mixtures thereof.

6. The skin composition of claim 1 or 2, in the form of a single-phase aqueous solution.

7. A skin composition comprising: 0.20 to 0.30% by weight of trisodium ascorbyl palmitate phosphate based on the total weight of the skin composition; 0.30 to 0.50% by weight of sodium ascorbyl phosphate based on the total weight of the skin composition; 0.010 to 0.020% by weight of polyquaternium-61 based on the total weight of the skin composition; 0.095 to 0.115% by weight of sodium hyaluronate based on the total weight of the skin composition; 4.00 to 5.00% by weight of polyols based on the total weight of the skin composition; and water, wherein the skin composition has a pH in the range of 6.0 to 8.5.

8. The skin composition of claim 7, in the form of a single-phase aqueous solution.

9. A cosmetic method comprising applying the skin composition of claim 1 or 7 on the entire face.

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