Biosensor
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Filing Date
- 2025-02-10
- Publication Date
- 2026-08-13
Smart Images
Figure KR2025001920_13082026_PF_FP_ABST
Abstract
Description
biosensor
[0001] The present invention relates to a biosensor, and more specifically, to a biosensor that reduces current drift caused by ion suspension within a fluid.
[0002] With the recent advancement of science and technology and the growing interest in the quality of life, the importance of disease diagnosis and prevention, as well as food and the environment, in human life is expanding day by day. As a result, there is an increasing need to measure the concentration of organic or inorganic substances in samples to diagnose human diseases, for specific processes in the fields of food chemistry and industrial chemistry, or to analyze pollutants in the environmental field, and many efforts are being made to address this.
[0003] A biosensor refers to a device that measures concentration by binding biomaterials, such as enzymes, microorganisms, antibodies, receptors, and DNA probes, to electrical or physicochemical transducers and detecting signals through electrochemical, optical, thermal, or piezoelectric methods based on electrode active substances or physical changes generated from reactions with the analyte to be measured.
[0004] Generally, the detection of biomaterials using biosensors is achieved through a combination of complex processes such as filtering, metering, mixing, transport, reaction, and washing. Therefore, conventionally, the detection of biomaterials is carried out manually at the laboratory level using various equipment.
[0005] Therefore, alongside the advancement of biosensor technology, the simultaneous development of fluid processing technology to automate and standardize the diagnostic process is crucial for low-cost, high-efficiency diagnosis.
[0006] Among these, equipment for detecting biomaterials requires flushing the internal flow paths before measuring each biological sample. Additionally, since large volumes of buffer solution must be stored in tanks to diagnose multiple samples, there is a risk of degradation, and there is the inconvenience of having to periodically empty the waste solution after diagnosis. Using such equipment presents limitations, as it may lead to reduced diagnostic speed and accuracy when a large volume of samples is required.
[0007] To solve this, a disposable biosensor cartridge with a built-in flow path through which buffer solution and sample solution can flow can be used. Since the disposable biosensor cartridge is used only once, a separate cleaning process is unnecessary, and since a single-use buffer solution is provided for each cartridge, there is no possibility of the buffer solution deteriorating, and the waste solution is emptied along with the cartridge when it is discarded.
[0008] In this regard, Korean published patent KR 10-2022-0047600A discloses a point-of-care molecular diagnostic system.
[0009] In the case of conventional diagnostic systems, there is a problem where ions inside the fluid remain suspended during repeated measurements, causing current drift or leakage current.
[0010] The present invention was created to improve upon the problems described above, and aims to provide a biosensor capable of reducing current drift and preventing leakage current by preventing the suspension of ions within a fluid.
[0011] The present invention was created to improve upon the problems described above, and aims to provide a biosensor capable of minimizing noise during testing and improving sensing precision or accuracy.
[0012] To achieve the above-mentioned purpose, the biosensor according to the present invention may include a substrate, a gate formed on the substrate, a drain formed on one side of the gate, a source formed on the other side of the gate, a drain-source channel formed between the drain and the source, a first reset switch with one end connected to the gate, a second reset switch with one end connected to the drain, a third reset switch with one end connected to the source, and a switching control unit that connects the other end of the first reset switch to ground or a gate electrode, connects the other end of the second reset switch to ground or a drain electrode, and connects the other end of the third reset switch to ground or a source electrode.
[0013] When a test start input signal is received, the switching control unit may connect the first reset switch to the gate electrode, connect the second reset switch to the drain electrode, and connect the third reset switch to the source electrode.
[0014] The switching control unit can apply power to the gate electrode when fluid is detected in the drain source channel.
[0015] The switching control unit can determine whether the transfer curve of the drain source channel is measured based on the gate voltage and drain current after applying power to the gate electrode.
[0016] When the transfer curve is measured, the switching control unit can connect the first reset switch, the second reset switch, and the third reset switch to ground.
[0017] To achieve the above-mentioned purpose, the biosensor cartridge according to the present invention comprises a fluid flow path forming portion, a biosensor disposed below the fluid flow path forming portion, and a sensor switching control portion, wherein the biosensor may include a graphene FET comprising a substrate, a gate, a source, a drain, and a drain-source channel, a first reset switch having one end connected to the gate and the other end connected to ground or a gate electrode, a second reset switch having one end connected to the drain and the other end connected to ground or a drain electrode, and a third reset switch having one end connected to the source and the other end connected to ground or a source electrode.
[0018] When a test start input signal is received, the sensor switching control unit may connect the first reset switch to the gate electrode, connect the second reset switch to the drain electrode, and connect the third reset switch to the source electrode.
[0019] The sensor switching control unit above can apply power to the gate electrode after flowing the fluid into the fluid-forming unit above.
[0020] The sensor switching control unit can determine whether the transfer curve of the drain source channel is measured based on the gate voltage and drain current after applying power to the gate electrode.
[0021] The sensor switching control unit can connect the first reset switch, the second reset switch, and the third reset switch to ground when the transfer curve is measured.
[0022] To achieve the above-mentioned purpose, the diagnostic system according to the present invention comprises a biosensor cartridge having a fluid-flowing channel formed therein, a biosensor disposed in the biosensor cartridge, and a diagnostic device for analyzing a signal received from the biosensor. The biosensor may include a substrate, an FET formed on the substrate and comprising a gate, a source, a drain, and a drain-source channel, a first reset switch having one end connected to the gate and the other end connected to ground or a gate electrode, a second reset switch having one end connected to the drain and the other end connected to ground or a drain electrode, and a third reset switch having one end connected to the source and the other end connected to ground or a source electrode.
[0023] When a test start input signal is received, the diagnostic device may connect the first reset switch to the gate electrode, connect the second reset switch to the drain electrode, and connect the third reset switch to the source electrode.
[0024] The above diagnostic device can apply power to the gate electrode after flowing the fluid through the above fluid path.
[0025] The diagnostic device can determine whether the transfer curve of the drain source channel is measured based on the gate voltage and drain current after applying power to the gate electrode.
[0026] When the transfer curve is measured, the diagnostic device can connect the first reset switch, the second reset switch, and the third reset switch to ground.
[0027] To achieve the above-mentioned purpose, a control method for a diagnostic device coupled with a biosensor cartridge including a biosensor according to the present invention may include the steps of: connecting a gate reset switch, a drain reset switch, and a source reset switch of the biosensor to ground; flowing fluid into a flow path of the biosensor cartridge when a test start input signal is received; connecting the gate reset switch to the gate electrode of the biosensor, connecting the drain reset switch to the drain electrode of the biosensor, and connecting the source reset switch to the source electrode of the biosensor; applying power to the gate electrode; determining whether a transfer curve of the drain-source channel of the biosensor is measured based on the gate voltage and drain current; and, when the transfer curve is measured, connecting the gate reset switch, the drain reset switch, and the source reset switch to ground.
[0028] By connecting the reset switch to ground (GND) before performing the test, noise during the test can be minimized, thereby improving the virus detection capability.
[0029] By connecting the reset switch to ground (GND) after the test is completed, the floating of ions within the fluid can be prevented, thereby preventing leakage of drain current (Ids) during repeated measurements (which can recover the charge trap of the liquid generated during sweeping), and thereby improving sensing precision or accuracy. In addition, by preventing the floating of ions within the fluid, current drift phenomena can be reduced and leakage current can be prevented.
[0030] By connecting the reset switch to ground (GND) after the test is completed, any unspecified potential difference remaining in the graphene FET can be removed without delay. Additionally, by preventing the floating of ions within the fluid, damage to the graphene FET caused by the influence of the electric field can be prevented.
[0031] FIG. 1 is a drawing for explaining a biosensor cartridge and a diagnostic device according to one embodiment of the present invention.
[0032] FIGS. 2 and FIGS. 3 are drawings for explaining a biosensor cartridge according to an embodiment of the present invention.
[0033] FIG. 4 is an exploded perspective view for explaining a frame according to one embodiment of the present invention.
[0034] FIG. 5 is a plan view for explaining a top frame according to one embodiment of the present invention.
[0035] FIG. 6 is a diagram illustrating the combination of a biosensor and a printed circuit board to a frame in a biosensor cartridge according to one embodiment of the present invention.
[0036] FIGS. 7 and 8 are cross-sectional views illustrating the arrangement of a biosensor and a printed circuit board and the arrangement of a flow path flowing over the biosensor in a biosensor cartridge according to an embodiment of the present invention.
[0037] FIG. 9 is a diagram illustrating the structure of a biosensor according to one embodiment of the present invention.
[0038] FIG. 10 is a circuit diagram of a biosensor according to one embodiment of the present invention.
[0039] FIG. 11 is a diagram illustrating the combined structure of a biosensor cartridge and a biosensor according to one embodiment of the present invention.
[0040] FIG. 12 is a diagram illustrating the structure of a biosensor according to one embodiment of the present invention.
[0041] FIG. 13 is a flowchart of a control method for a diagnostic device according to one embodiment of the present invention.
[0042] FIG. 14 is a block diagram of a biosensor cartridge according to one embodiment of the present invention.
[0043] FIG. 15 is a block diagram of a diagnostic system according to one embodiment of the present invention.
[0044] Preferred embodiments of the present invention will be described in detail below with reference to the attached drawings.
[0045] The present invention is capable of various modifications and may have various embodiments, and specific embodiments are illustrated in the drawings and described in detail in the detailed description. This is not intended to limit the present invention to specific embodiments, and should be interpreted to include all modifications, equivalents, and substitutions that fall within the spirit and scope of the invention.
[0046] In describing the present invention, terms such as "first," "second," etc., may be used to describe various components, but said components may not be limited by said terms. Such terms are used solely for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be named the second component, and similarly, the second component may be named the first component.
[0047] The term "and / or" may include a combination of multiple related listed items or any of the multiple related listed items.
[0048] When it is stated that one component is "connected" or "connected" to another component, it can be understood that while it may be directly connected or connected to that other component, there may also be other components in between. On the other hand, when it is stated that one component is "directly connected" or "directly connected" to another component, it can be understood that there are no other components in between.
[0049] The terms used in this application are used merely to describe specific embodiments and are not intended to limit the invention. Singular expressions may include plural expressions unless the context clearly indicates otherwise.
[0050] In this application, terms such as “comprising” or “having” are intended to indicate the existence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not excluding in advance the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.
[0051] Unless otherwise defined, all terms used herein, including technical or scientific terms, may have the same meaning as generally understood by those skilled in the art to which the present invention pertains. Terms such as those defined in commonly used dictionaries may be interpreted as having a meaning consistent with their meaning in the context of the relevant technology, and may not be interpreted in an ideal or overly formal sense unless explicitly defined in this application.
[0052] In addition, the following embodiments are provided to explain more completely to those with average knowledge in the industry, and the shapes and sizes of the elements in the drawings may be exaggerated for clearer explanation.
[0053]
[0054] FIG. 1 is a drawing for explaining a biosensor cartridge and a diagnostic device according to an embodiment of the present invention. FIG. 2 and FIG. 3 are drawings for explaining a biosensor cartridge according to an embodiment of the present invention. FIG. 4 is an exploded perspective view for explaining a frame according to an embodiment of the present invention. FIG. 5 is a plan view for explaining a top frame according to an embodiment of the present invention.
[0055] Referring to FIGS. 1 to 5, a biosensor cartridge (1) according to one embodiment of the present invention is coupled to a diagnostic device (2) to detect biomaterials and thereby diagnose diseases, etc. In one embodiment, the biosensor cartridge (1) may be inserted into the diagnostic device (2) in a state arranged along a horizontal direction. The biosensor cartridge (1) may allow a buffer solution and a sample solution to flow inside, thereby causing the biosensor (400) to undergo an electrochemical reaction with the biomaterials, and the resulting electrical change may be transmitted to the diagnostic device (2) through a printed circuit board (500).
[0056] A biosensor cartridge (1) according to one embodiment of the present invention may include a housing (100), a frame (200), a tank (300), a biosensor (400), a printed circuit board (500), a channel (600), a tube (700), a valve section (800), and a pump section (900).
[0057] A frame (200) is accommodated inside the housing (100), a tank (300) is formed on the upper side of the frame (200), and a channel (600) may be formed inside the frame (200). A tube (700), a valve section (800), and a pump section (900) are coupled to the frame (200), and a biosensor (400) and a printed circuit board (500) may be detachably coupled.
[0058] In the present invention, the direction in which the buffer solution is introduced relative to the frame (200) can be called the upper side, the direction opposite to the upper side relative to the frame (200) can be called the lower side, and the direction in which the biosensor cartridge (1) is inserted into the diagnostic device (2) can be called the front. That is, the direction in which the printed circuit board (500) is placed relative to the housing (100) can be called the front. Also, the direction opposite to the front can be called the rear. Additionally, when looking at the front from the rear end of the housing (100), the direction placed on the left can be called the left, and the direction placed on the right can be called the right.
[0059] The housing (100) forms the outer shape of the biosensor cartridge (1) and can accommodate a frame (200), a tank (300), a biosensor (400), a printed circuit board (500), a channel (600), a tube (700), a valve section (800), and a pump section (900) inside.
[0060] The housing (100) may include an upper housing (110) and a lower housing (120).
[0061] In one embodiment, the upper housing (110) is formed in the shape of a square box with the lower side open, and a buffer solution inlet hole (111), a sample solution inlet hole (112), a valve connection hole (113), and a pump connection hole (114) may be formed on the upper side.
[0062] The buffer solution inlet hole (111) can be formed to allow the buffer solution to flow in. The buffer solution inlet hole (111) can be formed in the blister receiving groove (111a).
[0063] A buffer blister (3) can be coupled to the blister receiving groove (111a). With this configuration, the buffer blister (3) can burst while being received inside the blister receiving groove (111a), allowing the buffer solution to pass through the buffer solution inlet hole (111) and flow into the buffer solution tank (310).
[0064] At least one ventilation hole (111b, 116) may be formed on the upper surface of the upper housing (110). This prevents the air pressure inside the buffer solution tank (310) from rising and reduces the probability of air mixing with the buffer solution.
[0065] A first ventilation channel (111c) may be further formed on the upper surface of the upper housing (110). The first ventilation channel (111c) is formed to communicate with the first ventilation hole (111b) and may be formed in a groove shape along the upper surface of the upper housing (110).
[0066] The sample solution inlet hole (112) can be formed to allow the sample solution to flow in.
[0067] The valve connection hole (113) can be formed so that a part of the valve part (800) passes through it.
[0068] The pump connection hole (114) can be formed so that a part of the pump section (900) passes through it.
[0069] A second ventilation hole (116) may be further formed on the upper surface of the upper housing (110). The second ventilation hole (116) may be positioned vertically above the waste solution tank (330). This prevents the air pressure inside the waste solution tank (330) from rising.
[0070] A second ventilation channel (116a) may be further formed on the upper surface of the upper housing (110). The second ventilation channel (116a) is formed to communicate with the second ventilation hole (116) and may be formed in a groove shape along the upper surface of the upper housing (110). Through this configuration, when a label (130) is attached to the upper surface of the upper housing (110), the second ventilation channel (116a) forms a space between the upper housing (110) and the label (130) to accommodate air discharged through the second ventilation hole (116).
[0071] The lower housing (120) can be combined with the upper housing (110) to form a space that accommodates a frame (200), a tank (300), a biosensor (400), a printed circuit board (500), a channel (600), a tube (700), a valve section (800), and a pump section (900) inside.
[0072] A sensor insertion hole (121) into which a biosensor (400) can be inserted may be formed on the lower side of the lower housing (120).
[0073] A sensor cover (122) can be attached to the lower side of the lower housing (120).
[0074] The sensor cover (122) may include a hook (122a), a sensor support (122b), and a coupling guide (not shown).
[0075] Referring to FIGS. 3 to 5, the frame (200) is placed inside the housing (100) and can form a channel (600) through which a buffer solution and a sample solution flow.
[0076] A biosensor (400) and a printed circuit board (500) are detachably coupled to the frame (200) so that the biosensor (400) can detect biomaterials in a sample solution flowing through a channel (600).
[0077] The frame (200) may include a base frame (210), a top frame (220), a hydrophilic adhesive layer (230), and a microchannel forming adhesive layer (240).
[0078] The base frame (210) can be detachably coupled to the biosensor (400).
[0079] The sensor coupling portion (211) can be detachably coupled to the biosensor (400).
[0080] A biosensor (400) can be fitted and coupled to the sensor coupling part (211).
[0081] A hole may be formed in the base frame (210) to fix the valve part (800) and the pump part (900), and a fixing member such as a screw may pass through the hole and be coupled with the valve part (800) and the pump part (900) positioned on the upper side of the top frame (220).
[0082] A tank (300) may be formed on the top frame (220). In one embodiment, a tank (300) may be formed on the upper surface of the top frame (220).
[0083] A channel (600) may be formed in the top frame (220). In one embodiment, a channel (600) may be formed on the lower surface of the top frame (220).
[0084] A substrate coupling portion (221) may be formed on the upper surface of the top frame (220). A printed circuit board (500) may be detachably coupled to the substrate coupling portion (221). The substrate coupling portion (221) may be positioned facing the sensor coupling portion (211).
[0085] The substrate coupling portion (221) may include a coupling guide portion (221a) that guides the slide coupling of the printed circuit board (500).
[0086] The substrate coupling portion (221) may further include a substrate support portion (221b) that guides the coupling position of the printed circuit board (500) and supports the printed circuit board (500). A pair of substrate support portions (221b) may be formed protruding upward from the upper surface of the top frame (220).
[0087] A clip receiving hole (221c) for receiving a contact clip (450) may be formed in the substrate coupling portion (221).
[0088] A pair of clip receiving holes (221c) can be formed to communicate with the sensor coupling part (211).
[0089] A tube receiving portion (222) may be formed in the top frame (220). The tube receiving portion (222) may be formed to protrude upward from the upper surface of the top frame (220).
[0090] The tube receiving portion (222) is formed to protrude in a block shape from the upper surface of the top frame (220), and a pump receiving groove (222a) and a tube guide groove (222b) may be formed inside.
[0091] The pump receiving groove (222a) can accommodate at least a portion of the tube (700) and the pump part (900) inside.
[0092] The tube guide groove (222b) is formed to communicate with the pump receiving groove (222a) and can accommodate at least a portion of the tube (700).
[0093] A valve coupling portion (223) may be formed on the top frame (220).
[0094] A buffer solution inlet port (315), a buffer solution port (615), a sample solution port (625), a sensing port (635), a prefill port (645), a waste solution port (655), a first tube connection port (660), and a second tube connection port (670) may be formed in the top frame (220).
[0095] The buffer solution port (615), sample solution port (625), sensing port (635) and prefill port (645) can be arranged to be connected by the valve section (800).
[0096] The first tube connection port (660) and the second tube connection port (670) may be provided to be connected to the tube (700).
[0097] The tank (300) is formed on the upper side of the frame (200) and can provide a space in which a buffer solution and / or a sample solution can be received.
[0098] The tank (300) may include a buffer solution tank (310).
[0099] A buffer solution inlet port (315) may be formed in the top frame (220), and the buffer solution inlet port (315) may be formed to communicate with the buffer solution channel (610).
[0100] The tank (300) may include a sample solution tank (320).
[0101] The tank (300) may include a waste solution tank (330).
[0102]
[0103] FIG. 6 is a diagram illustrating the combination of a biosensor and a printed circuit board to a frame in a biosensor cartridge according to an embodiment of the present invention. FIG. 7 and FIG. 8 are cross-sectional views illustrating the arrangement of a biosensor and a printed circuit board and the arrangement of a flow path flowing over the biosensor in a biosensor cartridge according to an embodiment of the present invention.
[0104] Referring to FIGS. 6 to 8, the biosensor (400) is detachably coupled to the frame (200) and can detect biomaterials. The biosensor (400) can be inserted through the sensor insertion hole (121) of the lower housing (120), and the biosensor (400) can be coupled to the sensor coupling part (211) of the base frame (210).
[0105] A biosensor (400) can selectively detect a minute amount of biomaterial to be analyzed by combining a bioreceptor having a recognition function for a specific biomaterial with an electrical transducer to convert biological interactions and recognition responses into electrical signals.
[0106] In one embodiment, a sensing portion may be provided on the upper surface of the biosensor (400). A bio-receptor may be disposed in the sensing portion. A buffer solution and a sample solution may flow through the sensing portion. The sensing portion may be connected to a circuit to transmit an electrical signal generated in the sensing portion.
[0107] A circuit may be placed on the biosensor (400) and may be electrically connected to the printed circuit board (500) by a contact clip (450).
[0108] A sealer (410) may be disposed on the upper side of the biosensor (400). The sealer (410) may be disposed on the sensor coupling portion (211). A flow path forming portion (411) may be formed in the sealer (410). In one embodiment, the sealer (410) may be formed in the shape of a rectangular parallelepiped, and the flow path forming portion (411) may be in the shape of a slit formed along the left and right directions.
[0109] The flow path forming part (411) may be positioned on the lower side of the frame formed in the sensor coupling part (211). The flow path forming part (411) may be positioned on the lower side of the inlet port (631a) and the outlet port (632a) formed in the sensor coupling part (211), and may be in communication with the inlet port (631a) and the outlet port (632a). The buffer solution and sample solution flowing through the first sensing channel (631) may flow through the flow path inside the flow path forming part (411) via the inlet port (631a), pass through the outlet port (632a), and be introduced into the second sensing channel (632).
[0110] The flow path forming part (411) may be positioned above the sensing part of the biosensor (400). The sealer (410) may be formed to surround the outer edge of the sensing part. The sealer (410) seals the outer edge of the flow path forming part (411) to prevent the buffer solution and sample solution flowing inside the flow path forming part (411) from leaking out.
[0111] The printed circuit board (500) can be detachably coupled to the frame (200). At least a portion of the biosensor (400) can be inserted into the upper housing (110) and detachably coupled to the substrate coupling portion (221) of the top frame (220). The printed circuit board (500) can be supported by the coupling guide portion (221a) and the substrate support portion (221b).
[0112] The printed circuit board (500) may include a board body (510), a connector (520), and a guide portion (530). In one embodiment, the board body (510) may be formed in the shape of a roughly rectangular flat plate. A circuit may be mounted on the board body (510) and electrically connected to the biosensor (400).
[0113] A connector (520) may be provided at the front end of the substrate body (510). The connector (520) may be connected to a circuit provided in the substrate body (510). When the biosensor cartridge (1) is coupled to the diagnostic device (2), an electrical signal can be transmitted to the diagnostic device (2) through the connector (520). When the biosensor cartridge (1) is coupled to the diagnostic device (2), power can be applied to the printed circuit board (500) and the biosensor (400) through the connector (520).
[0114] The guide portion (530) may be formed at both ends of the substrate body (510) in the left and right directions and may be coupled with the substrate support portion (221b) of the substrate coupling portion (221). In one embodiment, the guide portion (530) may be formed as a recessed shape in the left and right directions of the substrate body (510). Through this, it may be coupled with the protruding shape of the substrate support portion (221b) to guide the accurate coupling position of the printed circuit board (500) and stably support the printed circuit board (500) in the coupled state.
[0115] A contact clip (450) may be placed between the biosensor (400) and the printed circuit board (500). The contact clip (450) is coupled to the sensor coupling portion (211) and may come into contact with the printed circuit board (500) and the biosensor (400). The contact clip (450) is positioned to pass through the clip receiving hole (221c) and to pass through the hole of the sensor coupling portion (211), but at least a portion may be supported by the base frame (210).
[0116] A plurality of contact clips (450) may be provided. An even number of contact clips (450) may be provided. A plurality of pairs of contact clips (450) may be arranged side by side, and one pair may be symmetrically arranged in a position facing each other. In one embodiment, six contact clips (450) may be provided, and three pairs may be arranged side by side, and each pair may be symmetrically arranged in a position facing each other.
[0117] The contact clip (450) may be formed of a conductive material. In one embodiment, the contact clip (450) may be formed of a metal material. Each contact clip (450) may include a substrate contact portion (451), a sensor contact portion (452), and a connection portion (453).
[0118] The sensor contact portion (452) is positioned to pass through a hole formed in the sensor coupling portion (211) and can come into contact with a terminal (not shown) provided in the biosensor (400). That is, the sensor contact portion (452) can be electrically connected to a circuit mounted on the biosensor (400). In one embodiment, the sensor contact portion (452) may be in the form of a plate that is formed extending downward from the coupling portion (453), then folded and extended upward.
[0119] The total height of the contact clip (450) in the vertical direction can be formed to be longer than the shortest distance between the biosensor (400) and the printed circuit board (500). Through this, when the sensor contact portion (452) comes into contact with the biosensor (400), the sensor contact portion (452) can apply pressure to the biosensor (400) while elastically deforming, and can maintain a strong contact state while minimizing the contact area with the biosensor (400).
[0120] The connecting portion (453) may be formed to connect the substrate contact portion (451) and the sensor contact portion (452). The connecting portion (453) is formed by bending and extending downward from the substrate contact portion (451), then being bent and extended along the front-rear direction, and may be connected to the sensor contact portion (452) by bending and extending downward. The connecting portion (453) may be supported by contacting the base frame (210).
[0121] The contact clip (450) is seated on the frame (200), so that its upper side contacts the printed circuit board and its lower side contacts the biosensor (400). Through the contact clip (450), the buffer solution and sample solution can be flowed between the printed circuit board (500) and the biosensor (400), while the electrical signal generated from the biosensor (400) can be transmitted to the printed circuit board over the shortest distance.
[0122]
[0123] Referring to FIGS. 1 and 2, the biosensor cartridge (1) may have a biosensor (400) and a printed circuit board (500) combined therein. However, if there is an error in the biosensor (400) and the printed circuit board (500), the user may replace them.
[0124] The user can inject the buffer solution into the biosensor cartridge (1). The user can inject it into the buffer solution injection hole (111). In the present invention, the buffer solution can be injected into the buffer solution injection hole (111) using a buffer blister (3).
[0125] In one embodiment, the buffer blister (3) may be formed with a circular dome shape at the top and a flat shape at the bottom. The top of the buffer blister (3) may be formed of a deformable material, and the bottom surface of the buffer blister (3) may be formed of a tearable material.
[0126] The buffer blister (3) can be received in the blister receiving groove (111a). The buffer blister (3) may be provided in a fixed state in the blister receiving groove (111a), and the buffer blister (3) may also be detachably coupled to the blister receiving groove (111a).
[0127] With the buffer blister (3) placed inside the blister receiving groove (111a), the user can press the buffer blister (3) downward using a finger or the like. In this case, the upper part of the dome-shaped buffer blister (3) is deformed downward, and as the internal pressure of the buffer blister (3) increases, the lower surface bursts, and the buffer solution stored inside the buffer blister (3) flows downward due to gravity and can be introduced into the buffer solution injection hole (111).
[0128] The buffer solution that has passed through the buffer solution inlet hole (111) can be contained in the buffer solution tank (310). It can then flow into the buffer solution inlet port (315) along the inclined surface (311). Additionally, some of the buffer solution contained in the buffer solution tank (310) can flow along the buffer solution channel (610). However, unless the pump unit (900) is operating, it may not flow into the sensing channel (630).
[0129] The user can inject the sample solution into the sample solution inlet hole (112). The user can inject the sample solution into the sample solution inlet hole (112) using a sample injection tool (not shown) including a dropper.
[0130] It is possible to inject the buffer solution and the sample solution simultaneously, to inject the sample solution first and then the buffer solution, or to inject the buffer solution first and then the sample solution.
[0131] The sample solution that has passed through the sample solution inlet hole (112) can be received in the sample solution tank (320). And, some of the sample solution received in the sample solution tank (320) can flow along the sample solution channel (620). However, unless the pump unit (900) is operated, it may not flow into the sensing channel (630).
[0132] After the buffer solution and sample solution are introduced, the user can insert the biosensor cartridge (1) into the diagnostic device (2).
[0133]
[0134] FIG. 9 is a diagram illustrating the structure of a biosensor according to an embodiment of the present invention. FIG. 10 is a circuit diagram of a biosensor according to an embodiment of the present invention. FIG. 11 is a diagram illustrating the combined structure of a biosensor cartridge and a biosensor according to an embodiment of the present invention. FIG. 12 is a diagram illustrating the structure of a biosensor according to an embodiment of the present invention.
[0135] Referring to FIGS. 9 to 12, a biosensor (400) according to one embodiment of the present invention may be a graphene FET (Field effect transistor) (5) and may include a substrate (401), a first gate (421), a second gate (422), a drain (431), a source (432), a drain-source channel (433), a first reset switch (441), a second reset switch (442), a third reset switch (443), and a switching control unit (445).
[0136] As one embodiment, the substrate (401) may be in a single crystal state and may include silicon (Si) material. As one embodiment, the substrate (401) may be a thinned substrate through a thinning process.
[0137] The first gate (421) is formed on the substrate (401), and when a fluid (L) flows through the sensing channel (630) of the biosensor cartridge (1) and reaches the flow path forming part (411), the fluid (L), which is a buffer solution or a sample solution, can be contacted. The first gate (421) serves as a common gate electrode and can perform the role of applying an intended voltage to the solution in contact with the drain source channel (433).
[0138] The first gate (421) can be positioned or formed at a lower position of the inlet port (631a) formed in the base frame (210) of the biosensor cartridge (1) when coupled to the biosensor cartridge (1).
[0139] The second gate (422) is formed on the substrate (401) and may be in contact with a buffer solution or sample solution, which is a fluid (L).
[0140] The first gate (421) and the second gate (422) may, as one embodiment, include polysilicon or a metallic material (e.g., tungsten (W), copper (Cu), aluminum (Al), gold (Au), palladium (Pd)).
[0141] The first gate (421) and the second gate (422) can be formed by a deposition process such as photolithography patterning or CVD as in one embodiment.
[0142] The drain (431) may be formed on the substrate (401). The drain (431) may be placed or formed on one side of the gate (420).
[0143] The source (432) may be formed on the substrate (401). The source (432) may be placed or formed on the other side of the gate (420).
[0144] As one embodiment, the drain (431) and source (432) may include polysilicon or a metallic material (e.g., tungsten (W), copper (Cu), aluminum (Al), gold (Au), palladium (Pd)).
[0145] As an example, the drain (431) and source (432) can be formed by a deposition process such as photolithography patterning or CVD.
[0146] The drain source channel (433) is a graphene channel through which fluid (L) passes, and can be formed between the drain (431) and the source (432).
[0147] The drain source channel (433) may include graphene as in one embodiment. As in one embodiment, the drain source channel (433) may be formed through a patterning and graphene deposition process.
[0148] One end of the first reset switch (441) is electrically connected to the first gate (421), and the other end can be electrically connected to ground (GND) or the gate electrode (471) by a switching control signal.
[0149] One end of the second reset switch (442) is electrically connected to the drain (431), and the other end can be electrically connected to the ground (GND) or drain electrode (472) by a switching control signal.
[0150] One end of the third reset switch (443) is electrically connected to the source (432), and the other end can be electrically connected to the ground (GND) or the source electrode (473) by a switching control signal.
[0151] The switching control unit (445) may connect the other end of the first reset switch (441) to ground (GND) or the gate electrode (471), connect the other end of the second reset switch (442) to ground (GND) or the drain electrode (472), or connect the other end of the third reset switch (443) to ground (GND) or the source electrode (473).
[0152]
[0153] FIG. 13 is a flowchart of a control method for a diagnostic device according to one embodiment of the present invention.
[0154] Referring to FIG. 13, in step S110, the switching control unit (445) determines whether the first reset switch (441), the second reset switch (442), and the third reset switch (443) are connected to ground (GND) before receiving a test start input signal from the diagnostic device (2) or the biosensor cartridge (1), and if the first reset switch (441), the second reset switch (442), and the third reset switch (443) are not connected to ground (GND), the first reset switch (441), the second reset switch (442), and the third reset switch (443) can be connected to ground (GND) (S110). By connecting the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND) before performing the test, noise during the test can be minimized, and thereby, the virus detection capability can be improved.
[0155] In step S120, when the biosensor cartridge (1) receives a test start input signal from the diagnostic device (2), it can operate the pump unit (900) to flow fluid (L). The fluid (L) can reach the flow path forming unit (411) along the sensing channel (630).
[0156] In step S130, when the switching control unit (445) receives a test start input signal from the diagnostic device (2) or the biosensor cartridge (1), it may connect the other end of the first reset switch (441) connected to ground (GND) to the gate electrode (471), connect the other end of the second reset switch (442) connected to ground (GND) to the drain electrode (472), and connect the other end of the third reset switch (443) connected to ground (GND) to the source electrode (473).
[0157] The switching control unit (445) can apply power to the gate electrode (471) when fluid (L) is detected in the drain source channel (433). In one embodiment, the switching control unit (445) can detect that fluid (L) is reaching the drain source channel (433) using at least one of a pressure sensor, a temperature sensor, a flow sensor, and a flow rate sensor. It is not limited thereto, and the switching control unit (445) can detect that fluid (L) is reaching the drain source channel (433) through various types of sensors or methods. In one embodiment, the switching control unit (445) may connect the other end of the first reset switch (441) to the gate electrode (471), connect the other end of the second reset switch (442) to the drain electrode (472), and connect the other end of the third reset switch (443) to the source electrode (473), and then apply power to the gate electrode (471) even before fluid (L) is detected in the drain source channel (433).
[0158] In step S140, the switching control unit (445) can determine whether the transfer curve (Tr-curve) of the drain source channel (433) is measured based on the gate voltage and drain current after power is applied to the gate electrode (471). The transfer curve may represent the correlation between the gate voltage (Vgs) and the drain current (Ids).
[0159] In step S150, when the transfer curve is measured, the switching control unit (445) can connect the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND). The completion of the test can be determined through the measurement of the transfer curve, and after the test is completed, by connecting the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND), the floating of ions inside the fluid (L) can be prevented, thereby preventing leakage of drain current (Ids) during repeated measurements (the charge trap of the liquid generated during sweeping can be recovered), and thereby the sensing precision or accuracy can be improved. In addition, by preventing the floating of ions inside the fluid, the current drift phenomenon can be reduced and leakage current can be prevented. In addition, by connecting the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND) after the test is completed, any unspecified potential difference remaining in the graphene FET can be removed without delay. In addition, by preventing the floating of ions inside the fluid (L), damage to the graphene FET caused by the electric field can be prevented.
[0160]
[0161] FIG. 14 is a block diagram of a biosensor cartridge according to one embodiment of the present invention.
[0162] Referring to FIG. 14, the biosensor cartridge (1) may include a flow path forming part (411), a biosensor (400), and a sensor switching control part (455).
[0163] As an example, the biosensor (400) may include a graphene FET (5), a first reset switch (441), a second reset switch (442), and a third reset switch (443).
[0164] The graphene FET (5) may include a substrate (401), a first gate (421), a second gate (422), a drain (431), a source (432), and a drain-source channel (433).
[0165] The sensor switching control unit (455) determines whether the first reset switch (441), the second reset switch (442), and the third reset switch (443) are connected to ground (GND) before receiving a test start input signal from the diagnostic device (2), and if the first reset switch (441), the second reset switch (442), and the third reset switch (443) are not connected to ground (GND), the first reset switch (441), the second reset switch (442), and the third reset switch (443) can be connected to ground (GND). By connecting the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND) before performing the test, noise during the test can be minimized, and thereby, the virus detection capability can be improved.
[0166] When the sensor switching control unit (455) receives a test start input signal from the diagnostic device (2), it can operate the pump unit (900) to flow the fluid (L). The fluid (L) can reach the flow path forming unit (411) along the sensing channel (630).
[0167] When the sensor switching control unit (455) receives a test start input signal from the diagnostic device (2), it can connect the other end of the first reset switch (441) connected to ground (GND) to the gate electrode (471), connect the other end of the second reset switch (442) connected to ground (GND) to the drain electrode (472), and connect the other end of the third reset switch (443) connected to ground (GND) to the source electrode (473).
[0168] The sensor switching control unit (455) can apply power to the gate electrode (471) after fluid (L) is flowed into the flow path forming unit (411) and fluid (L) is detected in the drain source channel (433) of the biosensor (400). The sensor switching control unit (455) can detect that fluid (L) reaches the drain source channel (433) using at least one of a pressure sensor, a temperature sensor, a flow rate sensor, and a flow velocity sensor. It is not limited thereto, and the sensor switching control unit (445) can detect that fluid (L) reaches the drain source channel (433) through various types of sensors or methods. As an example, the sensor switching control unit (455) can apply power to the gate electrode (471) after fluid (L) is flowed into the flow path forming unit (411) even before fluid (L) is detected in the drain source channel (433).
[0169] After applying power to the gate electrode (471), the sensor switching control unit (455) can determine whether the transfer curve (Tr-curve) of the drain source channel (433) is measured based on the gate voltage and drain current.
[0170] When the transfer curve is measured, the sensor switching control unit (455) can connect the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND). The test completion status can be determined through the measurement of the transfer curve, and after the test is completed, by connecting the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND), the floating of ions inside the fluid (L) can be prevented, thereby preventing leakage of drain current (Ids) during repeated measurements (the charge trap of the liquid generated during sweeping can be restored), and thereby the sensing precision or accuracy can be improved. In addition, by connecting the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND) after the test is completed, any unspecified potential difference remaining in the graphene FET (5) can be removed without delay. In addition, by preventing the floating of ions inside the fluid (L), damage to the graphene FET caused by the electric field can be prevented.
[0171]
[0172] FIG. 15 is a block diagram of a diagnostic system according to one embodiment of the present invention.
[0173] Referring to FIG. 15, the diagnostic system may include a biosensor cartridge (1), a biosensor (400), and a diagnostic device (2).
[0174] As an example, the biosensor (400) may include a graphene FET (5), a first reset switch (441), a second reset switch (442), and a third reset switch (443).
[0175] The graphene FET (5) may include a substrate (401), a first gate (421), a second gate (422), a drain (431), a source (432), and a drain-source channel (433).
[0176] The diagnostic device control unit (26) of the diagnostic device (2) determines whether the first reset switch (441), the second reset switch (442), and the third reset switch (443) are connected to ground (GND) before a test start signal is input by a user through the operation unit (21a) or before a test start input signal is received by a user terminal (not shown). If the first reset switch (441), the second reset switch (442), and the third reset switch (443) are not connected to ground (GND), the first reset switch (441), the second reset switch (442), and the third reset switch (443) can be connected to ground (GND). By connecting the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND) before performing the test, noise during the test can be minimized, and thereby, the virus detection capability can be improved.
[0177] When a test start signal is input by a user through the operating unit (21a) or a test start input signal is received by a user terminal (not shown), the diagnostic device control unit (26) can operate the pump unit (900) of the biosensor cartridge (1) to flow fluid (L). The fluid (L) can reach the flow path forming unit (411) along the sensing channel (630) which is the flow path.
[0178] When a test start signal is input by a user through the operating unit (21a) or a test start input signal is received by a user terminal (not shown), the diagnostic device control unit (26) may connect the other end of a first reset switch (441) connected to ground (GND) to a gate electrode (471), connect the other end of a second reset switch (442) connected to ground (GND) to a drain electrode (472), and connect the other end of a third reset switch (443) connected to ground (GND) to a source electrode (473).
[0179] The diagnostic device control unit (26) can apply power to the gate electrode (471) after the fluid (L) is flowed into the fluid forming unit (411) along the sensing channel (630) of the biosensor (400) and the fluid (L) is detected in the drain source channel (433) of the biosensor (400). The diagnostic device (2) can detect when the fluid (L) reaches the drain source channel (433) using at least one of a pressure sensor, a temperature sensor, a flow sensor, and a flow rate sensor. It is not limited thereto, and the diagnostic device control unit (26) can detect when the fluid (L) reaches the drain source channel (433) through various types of sensors or methods. As an example, the diagnostic device control unit (26) can apply power to the gate electrode (471) after the fluid (L) is flowed into the fluid forming unit (411) even before the fluid (L) is detected in the drain source channel (433).
[0180] After applying power to the gate electrode (471), the diagnostic device control unit (26) can determine whether the transfer curve (Tr-curve) of the drain source channel (433) is measured based on the gate voltage and drain current.
[0181] When the transfer curve is measured, the diagnostic device control unit (26) can connect the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND). The test completion status can be determined through the measurement of the transfer curve, and after the test is completed, by connecting the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND), the floating of ions inside the fluid (L) can be prevented, thereby preventing leakage of drain current (Ids) during repeated measurements (the charge trap of the liquid generated during sweeping can be restored), and thereby the sensing precision or accuracy can be improved. In addition, by connecting the first reset switch (441), the second reset switch (442), and the third reset switch (443) to ground (GND) after the test is completed, any unspecified potential difference remaining in the graphene FET (5) can be removed without delay. In addition, by preventing the floating of ions inside the fluid (L), damage to the graphene FET caused by the electric field can be prevented.
[0182] The switching control operation of the first reset switch (441), the second reset switch (442), and the third reset switch (443) may be performed by the switching control unit (445), but is not limited thereto. As described above, it may be performed by the sensor switching control unit (455) of the biosensor cartridge (1) or the diagnostic device control unit (26) of the diagnostic device (2), and the effect of the switching control operation of the first reset switch (441), the second reset switch (442), and the third reset switch (443) may be the same.
[0183]
[0184] Although the present invention has been described in detail through specific embodiments, this is for the purpose of specifically explaining the invention and is not limited thereto. It is evident that modifications or improvements to the present invention are possible by those skilled in the art within the technical scope of the invention.
[0185] All simple variations or modifications of the present invention fall within the scope of the present invention, and the specific scope of protection of the present invention will be clarified by the appended claims.
Claims
1. Substrate, A gate formed on the above substrate, A drain formed on one side of the above gate, A source formed on the other side of the above gate, A drain source channel formed between the drain and the source, A first reset switch connected to the above gate, A second reset switch connected to the above drain, A third reset switch connected to the above source, and A switching control unit that connects the other end of the first reset switch to ground or a gate electrode, connects the other end of the second reset switch to ground or a drain electrode, and connects the other end of the third reset switch to ground or a source electrode. A biosensor including 2. In Paragraph 1, The above switching control unit is, A biosensor that, when a test start input signal is received, connects the first reset switch to the gate electrode, connects the second reset switch to the drain electrode, and connects the third reset switch to the source electrode.
3. In Paragraph 2, The above switching control unit is, A biosensor that applies power to the gate electrode when fluid is detected in the drain source channel.
4. In Paragraph 3, The above switching control unit is, A biosensor that determines whether a transfer curve of the drain-source channel is measured based on the gate voltage and drain current after applying power to the gate electrode.
5. In Paragraph 4, The above switching control unit is, A biosensor that connects the first reset switch, the second reset switch, and the third reset switch to ground when the above transfer curve is measured.
6. A channel forming part through which fluid flows, A biosensor disposed below the above-mentioned Euro forming part, and Sensor switching control unit Includes, The above biosensor is, A graphene FET comprising a substrate, a gate, a source, a drain, and a drain-source channel, A first reset switch, one end of which is connected to the gate and the other end of which is connected to ground or the gate electrode, A second reset switch, one end of which is connected to the drain and the other end of which is connected to the ground or drain electrode, and A third reset switch, one end of which is connected to the above source and the other end of which is connected to ground or the source electrode. A biosensor cartridge containing 7. In Paragraph 6, The sensor switching control unit above is, A biosensor cartridge that, when a test start input signal is received, connects the first reset switch to the gate electrode, connects the second reset switch to the drain electrode, and connects the third reset switch to the source electrode.
8. In Paragraph 7, The sensor switching control unit above is, A biosensor cartridge that applies power to the gate electrode after flowing the fluid into the above-mentioned Euro forming portion.
9. In Paragraph 8, The sensor switching control unit above is, A biosensor cartridge that determines whether a transfer curve of the drain-source channel is measured based on the gate voltage and drain current after applying power to the gate electrode.
10. In Paragraph 9, The sensor switching control unit above is, A biosensor cartridge that connects the first reset switch, the second reset switch, and the third reset switch to ground when the above transfer curve is measured.
11. Biosensor cartridge in which a fluid flow path is formed, A biosensor disposed in the above biosensor cartridge, and Diagnostic device for analyzing signals received from the above biosensor Includes, The above biosensor is, substrate, A FET formed on the above substrate and comprising a gate, a source, a drain, and a drain-source channel, A first reset switch, one end of which is connected to the gate and the other end of which is connected to ground or the gate electrode, A second reset switch, one end of which is connected to the drain and the other end of which is connected to the ground or drain electrode, and A third reset switch, one end of which is connected to the above source and the other end of which is connected to ground or the source electrode. A diagnostic system including 12. In Paragraph 11, The above diagnostic device is, A diagnostic system that, when a test start input signal is received, connects the first reset switch to the gate electrode, connects the second reset switch to the drain electrode, and connects the third reset switch to the source electrode.
13. In Paragraph 12, The above diagnostic device is, A diagnostic system that applies power to the gate electrode after flowing the fluid through the above-mentioned Euro.
14. In Paragraph 13, The above diagnostic device is, A diagnostic system that determines whether a transfer curve of the drain-source channel is measured based on the gate voltage and drain current after applying power to the gate electrode.
15. In Paragraph 14, The above diagnostic device is, A diagnostic system that connects the first reset switch, the second reset switch, and the third reset switch to ground when the above transfer curve is measured.
16. A method for controlling a diagnostic device combined with a biosensor cartridge including a biosensor, wherein A step of connecting the gate reset switch, drain reset switch, and source reset switch of the biosensor to ground, When a test start input signal is received, a step of flowing fluid into the flow path of the biosensor cartridge, A step of connecting the gate reset switch to the gate electrode of the biosensor, connecting the drain reset switch to the drain electrode of the biosensor, and connecting the source reset switch to the source electrode of the biosensor. Step of applying power to the gate electrode, A step of determining whether a transfer curve of the drain source channel of the biosensor is measured based on the gate voltage and drain current, and When the above transfer curve is measured, the step of connecting the gate reset switch, the drain reset switch, and the source reset switch to ground. A method including