Method of obtaining usable water with probiotic bacteria-based biomaterial

Abstract

The invention relates to a method of obtaining usable water with a low-cost and environmentally friendly probiotic bacteria-based biomaterial, which removes organic contaminants without requiring a chemical process by subjecting wastewater (sewage water) to biological treatment in aerobic and anaerobic environments using the probif biomaterial, which contains prebiotic xylo-oligosaccharide, probiotic bifidobacterium infantis and postbiotic sodium alginate, reduces heavy metals and toxic compounds, makes the pH and turbidity levels suitable for agricultural irrigation, and also enables the solid organic component waste formed during the treatment process to be used as a potential fertilizer.

Description

[0001] DESCRIPTION

[0002] METHOD OF OBTAINING USABLE WATER WITH PROBIOTIC BACTERIA- BASED BIOMATERIAL

[0003] Technical Field

[0004] The invention relates to a method of obtaining usable water with probiotic bacteria-based biomaterial, which provides an environmentally friendly and economical solution without using chemicals and operating with natural biodegradation mechanisms compared to traditional water treatment systems, and effectively decomposes organic pollutants in sewage waste thanks to the metabolic activities of probiotic bacteria and makes dirty water reusable for irrigation purposes.

[0005] Background of the Invention

[0006] The basic components of the existing wastewater treatment systems have remained largely unchanged for 80-100 years. The conventional treatment methods include physical, chemical and biological treatment processes. However, as a result of the new technological developments, various chemicals and synthetic residues, increasing population, urbanization and industrial activities, the content of wastewater has become complex and the existing systems have become inadequate in the removal of the new generation pollutants.

[0007] Some substances that complicate the content of wastewater and make it difficult to be treated can be classified as follows:

[0008] • Use of synthetic detergents and phosphates: Detergents, which have become widespread in domestic and industrial use, contain chemical components that do not easily biodegrade in the aquatic environment.

[0009] • Toxic organic chemicals: Chemicals used in modern cleaning and industrial processes produce organic residues that current biological treatment systems cannot decompose.• Heavy metals and industrial pollutants: Heavy metals from the metal processing, mining, paint and textile industries cause permanent pollution in the aquatic environment.

[0010] In the current treatment systems, the following methods are generally used:

[0011] • Physical treatment: Settling of sediments, filtration and retention of coarse waste.

[0012] • Chemical treatment: Removal of the pollution using certain chemical methods such as coagulation, flocculation and disinfection.

[0013] • Biological treatment: Decomposition of organic substances with the help of microorganisms.

[0014] Although each of these methods has certain advantages, their biggest disadvantages are that they are chemical and energy intensive. In particular, by-products and wastes resulting from the chemical treatment methods can pose a great risk to the ecosystem.

[0015] The main problems of the treatment methods in the current systems are:

[0016] • High Cost: Very costly due to the use of chemicals and certain energy requirement.

[0017] • Chemical waste generation: The chemicals used can harm the aquatic ecosystems.

[0018] • Inefficient treatment: Micropollutants and some organic components fail to be completely cleaned.

[0019] • High carbon footprint: Conventional treatment plants cause large-scale energy use and therefore high greenhouse gas emissions.

[0020] On the other hand, although not preferred very often, the biological water treatment methods are also used. The advantages of the biological treatment methods can be listed as follows:

[0021] • being low cost and energy efficient,

[0022] • can purify water naturally without using chemicals,

[0023] • incorporating organic matter into the recycling process by supporting the nutrient cycle.However, the traditional biological treatment methods are not sufficiently effective on the synthetic organic compounds. In addition, the systems operate slowly and sufficient quality cannot be obtained for direct useable water (irrigation water) from wastewater (sewage waste / water).

[0024] On the other hand, biotechnology-based approaches attract attention both in terms of sustainability and lower operating costs in this field. The invention of the method of obtaining usable water with probiotic bacteria-based biomaterials, which was developed to completely eliminate the disadvantages of the current methods, provides a chemical-free and environmentally friendly treatment by transforming the harmful components in sewage waste through natural biological processes using probiotic bacteria and plant-based biomaterials.

[0025] The invention is based on the dissolution of organic and inorganic wastes by probiotics and biological disinfection to improve water quality, while also offering a new approach to the fields of waste management and agricultural irrigation. Unlike traditional methods, it is sustainable because it works with low energy consumption and does not require any additional chemical processing.

[0026] The advantages of the invention of the method of obtaining usable water with probiotic bacteria-based biomaterial, which makes the biological treatment process more effective with the use of probiotic bacteria, can be summarized as follows:

[0027] • There is no use of chemicals and the treatment is done using a completely biological method. It is ecologically friendly as the biological transformation is achieved without the use of chemicals.

[0028] • Thanks to the probiotic bacteria, the biological treatment speed increases and the wastewater is cleaned in a shorter time.

[0029] • The bacteria-based biodegradation enables the removal of chemical pollutants from water.Description of the Invention

[0030] In this detailed description, the subject of the invention relates to the method obtaining usable water with probiotic bacteria-based biomaterial and is explained only for the purpose of a better understanding of the subject and in a way that does not create any limiting effect.

[0031] The preparation method steps of the invention are as follows:

[0032] • taking 1000 mL samples from the sewer or domestic wastewater source, • preserving the samples in a sterile and controlled environment during the experiment,

[0033] • creating the experimental groups,

[0034] creating a control group with 1000 mL of raw sewage wastewater without any treatment,

[0035] creating an anaerobic test group with 1000 mL of wastewater sample kept in a partially anaerobic environment by adding the probif powder, creating an aerobic experimental group with 1000 mL of wastewater sample kept in an aerobic environment by adding the probif powder, • adding the probif biomaterial,

[0036] adding 1-100% of the probif powder containing prebiotic xylooligosaccharide, probiotic bifidobacterium infantis and postbiotic sodium alginate composite to each test sample,

[0037] • starting the experiment process,

[0038] keeping the samples under specified conditions for 7 days, keeping the anaerobic group in a closed and oxygen-free environment, keeping the aerobic group in an environment with constant oxygen contact,

[0039] • making daily observations and measurements,

[0040] monitoring the pH changes and physical parameters,

[0041] monitoring turbidity and organic matter load,

[0042] • assessing the treated water and the waste generated at the end of the experiment,Performing the water phase separation and chemical analysis of the obtained water,

[0043] Performing the separation of the solid waste phase (mucilage) and analysis to be used as fertilizer,

[0044] • Determining whether the treated water is suitable for irrigation under the desired conditions,

[0045] Measuring the pH value (ideal: 6-7.5),

[0046] measuring the turbidity level,

[0047] determining the conductivity (EC < 3 dS / m),

[0048] determining the chemical oxygen demand (COD) and biological oxygen demand (BOD) levels,

[0049] determining the heavy metal content (absence of toxic elements such as Pb, Cd, Hg, As),

[0050] measuring the microbiological load (total coliform bacteria level), measuring the amount of suspended solids,

[0051] determining the total dissolved solids,

[0052] determining the sodium adsorption rate (should be 18-26%), determining the nitrogen load (should be 10-30%),

[0053] determining the irrigation water class, (must be suitable for C1 S3, C2S3, C3S3, C3S2, C3S1 classes)

[0054] • evaluating the results,

[0055] determining whether the treated water is suitable for use in agriculture, examining the suitability of the resulting solid waste (mucilage) for use as fertilizer by evaluating its organic matter content, carbon / nitrogen ratio and nutrient elements.

[0056] The invention in question consists of:

[0057] • probif in powder form at a rate of 1 -100% in 1000ml wastewater (sewage water), • bifidobacterium-specific prebiotic xylo-oligosaccharide,

[0058] • probiotic bifidobacterium infantis,

[0059] • postbiotic sodium alginate.It is obvious that a person specialized about the technique may reveal the innovation specified in this invention by means of using similar structures and / or implements this structure in other areas with similar purposes used in the respective technique. Therefore, it is obvious that such attempts would fail to fulfill the criteria to get considered an invention.

Claims

CLAIMS1. The invention relates to a method of obtaining usable water with probiotic bacteria-based biomaterial, and is characterized by:• taking 1000 mL samples from the sewer or domestic wastewater source, • preserving the samples in a sterile and controlled environment during the experiment,• creating the experimental groups,✓ creating a control group with 1000 mL of raw sewage wastewater without any treatment,creating an anaerobic test group with 1000 mL of wastewater sample kept in a partially anaerobic environment by adding the probif powder, creating an aerobic experimental group with 1000 mL of wastewater sample kept in an aerobic environment by adding the probif powder, • adding the probif biomaterial,adding 1-100% of the probif powder containing prebiotic xylooligosaccharide, probiotic bifidobacterium infantis and postbiotic sodium alginate composite to each test sample,• starting the experiment process,keeping the samples under specified conditions for 7 days, keeping the anaerobic group in a closed and oxygen-free environment,keeping the aerobic group in an environment with constant oxygen contact,• making daily observations and measurements,monitoring the pH changes and physical parameters, monitoring turbidity and organic matter load,• assessing the treated water and the waste generated at the end of the experiment,Performing the water phase separation and chemical analysis of the obtained water,Performing the separation of the solid waste phase (mucilage) and analysis to be used as fertilizer,• Determining whether the treated water is suitable for irrigation under the desired conditions,Measuring the pH value (ideal: 6-7.5),measuring the turbidity level,determining the conductivity (EC < 3 dS / m),determining the chemical oxygen demand (COD) and biological oxygen demand (BOD) levels,determining the heavy metal content (absence of toxic elements such as Pb, Cd, Hg, As),measuring the microbiological load (total coliform bacteria level), measuring the amount of suspended solids,determining the total dissolved solids,determining the sodium adsorption rate (should be 18-26%), determining the nitrogen load (should be 10-30%),determining the irrigation water class, (must be suitable for C1S3, C2S3, C3S3, C3S2, C3S1 classes)• evaluating the results,determining whether the treated water is suitable for use in agriculture,examining the suitability of the resulting solid waste (mucilage) for use as fertilizer by evaluating its organic matter content, carbon / nitrogen ratio and nutrient elements.

2. The invention relates to a method of obtaining usable water with probiotic bacteria-based biomaterial, and is characterized by:• probif in powder form at a rate of 1-100% in 1000ml wastewater (sewage water),• bifidobacterium-specific prebiotic xylo-oligosaccharide,• probiotic bifidobacterium infantis,• postbiotic sodium alginate.