Composition having inhibitory activity against ages and preparation method therefor

Abstract

Provided are a composition having inhibitory activity against advanced glycation end products (AGEs) and a preparation method therefor. The composition comprises a camellia oleifera extract, banana flowers, Yunnan large-leaf sun-dried raw tea, and rose flowers. The preparation method therefor comprises: first mixing the banana flowers and the Yunnan large-leaf sun-dried raw tea for pile fermentation, then adding the camellia oleifera extract for further sealing and storage, and finally adding the rose flowers for aging treatment.

Description

A composition with AGEs inhibitory activity and its preparation method Technical Field

[0001] This invention relates to the application field of camellia oil extract, specifically to a composition with AGEs inhibitory activity and its preparation method. Background Technology

[0002] Advanced glycation end products (AGEs) are products of the binding of excess sugar and protein. They originate in the body from two sources: the synthesis of AGEs from excess sugar and protein within the body, and the ingestion of AGEs present in food. AGEs can combine with and damage body cells, accelerating aging and leading to many chronic degenerative diseases. Technical issues

[0003] Therefore, researching products with inhibitory activity against AGEs is of great significance for preventing aging and other health problems. Technical solutions

[0004] This invention provides a composition with AGEs inhibitory activity and a method for preparing the same, which has significant inhibitory activity against advanced glycation end products.

[0005] The technical solution of this invention is implemented as follows:

[0006] A composition having AGEs inhibitory activity comprises the following parts by weight: 1-5 parts of camellia oil extract, 10-20 parts of banana flower, 5-15 parts of Yunnan large-leaf sun-dried green tea, and 5-10 parts of rose.

[0007] Furthermore, the composition comprises the following parts by weight: 3 parts of camellia oil extract, 15 parts of banana flower, 10 parts of Yunnan large-leaf sun-dried green tea, and 8 parts of rose.

[0008] Furthermore, the banana flowers mentioned are freshly picked banana flowers, and the roses mentioned are freshly picked roses.

[0009] Furthermore, the preparation method of the camellia oil extract includes the following steps:

[0010] Degreased and dried camellia seed cake is then added to 5-6 times its weight of water and heated and boiled for 20-30 minutes to remove insoluble matter and obtain filtrate. The filtrate is then dried to obtain the final product.

[0011] A method for preparing a composition having AGEs inhibitory activity includes the following steps:

[0012] Freshly picked banana flowers and Yunnan large-leaf sun-dried green tea leaves were taken separately. After removing impurities, washing and drying, the banana flowers were cut into thin strips. Then, they were withered, fixed, rolled and sun-dried separately for later use.

[0013] Take sun-dried banana flowers and Yunnan large-leaf sun-dried green tea, mix them evenly to obtain a mixture; place the mixture in a room to ferment for 40-60 days.

[0014] The camellia oil extract is mixed with water to form a suspension, which is then added to the mixture and mixed evenly to form a mixture. After drying to a moisture content of 20-30%, it is stored in a sealed room away from light for 1-3 days.

[0015] After sealing and storing, dry it; then add dried rose petals, mix well, and age for at least 30 days to obtain the final product.

[0016] Furthermore, in step (1), the banana flowers are cut or torn into thin strips 0.5-1.0cm wide; the banana flowers and Yunnan large-leaf sun-dried green tea are spread on bamboo mats or bamboo sieves and placed in a well-ventilated indoor or outdoor shady place to wither until the moisture content of the banana flowers and Yunnan large-leaf sun-dried green tea reaches 60-70%; the temperature is controlled at 180-220℃ during the fixation process; the tea is kneaded until the strips are tightly rolled and a little juice seeps out; the sun-drying operation is as follows: spread the kneaded banana flowers and Yunnan large-leaf sun-dried green tea 3-5 cm apart and choose a sunny and well-ventilated place to dry until the moisture content drops to below 10%.

[0017] Furthermore, in step (2), during the fermentation process of the mixture, the temperature in the room is controlled at 30-40℃ and the humidity at 70-85%, and the material is turned over 2-3 times a day.

[0018] Furthermore, in step (3), during the sealed storage of the mixture, the room temperature is controlled at 25-35℃ and the humidity at 70-80%. The height of the mixture during stacking is 50-70 cm. The mixture is turned over once every 2 days. After the turning is completed, the room is sealed again.

[0019] Furthermore, in step (4), the mixture is dried to a moisture content of no more than 14%; the drying is carried out by baking; and the baking temperature does not exceed 60°C.

[0020] The above-described composition is used in the preparation of formulations that inhibit advanced glycation end products (AGEs).

[0021] An formulation capable of inhibiting advanced glycation end products, comprising the composition described above. Beneficial effects

[0022] The composition with AGEs inhibitory activity described in this invention involves first mixing and piling banana flowers and Yunnan large-leaf sun-dried green tea, allowing the banana flowers and Yunnan large-leaf sun-dried green tea to absorb each other's aromas during the mixing process. During the piling process, microorganisms in the Yunnan large-leaf sun-dried green tea facilitate the initial fermentation of the active ingredients in the banana flowers. Then, camellia oil extract is added for further sealed preservation, and finally, rose petals are added for aging treatment. This process enhances the composition's inhibitory activity against advanced glycation end products (AGEs), enriches the development of products with AGEs inhibitory activity, provides new ideas for the development of AGEs inhibitors, and offers new insights into the application of camellia oil extract, thus enriching the research and development of camellia oil-related products. Embodiments of the present invention

[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention. Example 1

[0024] A composition having AGEs inhibitory activity comprises the following components in parts by weight: camellia oil extract, banana flower, Yunnan large-leaf sun-dried green tea, and rose.

[0025] (1) Take freshly picked banana flowers and Yunnan large-leaf sun-dried green tea respectively, remove impurities, wash and dry them, then cut (cut or tear) the banana flowers into thin strips 0.5-1.0cm wide, and then wither, fix, roll and sun-dry them respectively for later use.

[0026] The withering process is as follows: banana flowers and Yunnan large-leaf sun-dried green tea are spread out on bamboo mats or bamboo sieves and placed in a well-ventilated, shaded indoor or outdoor place to wither until the moisture content of banana flowers and Yunnan large-leaf sun-dried green tea reaches 60-70%.

[0027] During the blanching process, control the temperature at 180℃; knead until the strips are tightly curled and a little juice seeps out.

[0028] The sun-drying process involves spreading the kneaded banana flowers and Yunnan large-leaf sun-dried green tea leaves 3-5 cm apart and drying them in a sunny and well-ventilated area until the moisture content drops below 10%.

[0029] (2) Take the sun-dried banana flowers and Yunnan large-leaf sun-dried green tea, mix them evenly to obtain a mixture; place the mixture in a room for fermentation for 40 days; during the fermentation process, control the room temperature at 30℃ and the humidity at 70-85%; enter the room twice a day to turn the mixture over.

[0030] (3) Add water to the camellia oil extract to make a suspension, add it to the mixture and mix evenly to make a mixture. Then dry it to a moisture content of 20%, and store it in a sealed room away from light for 1 day. During the sealed storage of the mixture, control the temperature in the room to be 25-35℃ and the humidity to be 70-80%. The height of the mixture during the stacking process is 50-70 cm. Enter the room every 2 days to turn the mixture over. After turning the mixture over, continue to seal the room.

[0031] (4) After sealing and preservation, dry it by drying until the moisture content is no more than 14% and the drying temperature is no more than 60℃; then add rose petals, mix evenly, and place it in a well-ventilated and dry environment to age for 30 days to obtain the product. Example 2

[0032] A composition having AGEs inhibitory activity comprises the following parts by weight: 3 parts of camellia oil extract, 15 parts of banana flower, 10 parts of Yunnan large-leaf sun-dried green tea, and 8 parts of rose.

[0033] (1) Take freshly picked banana flowers and Yunnan large-leaf sun-dried green tea respectively, remove impurities, wash and dry them, then cut (cut or tear) the banana flowers into thin strips 0.5-1.0cm wide, and then wither, fix, roll and sun-dry them respectively for later use.

[0034] The withering process is as follows: banana flowers and Yunnan large-leaf sun-dried green tea are spread out on bamboo mats or bamboo sieves and placed in a well-ventilated, shaded indoor or outdoor place to wither until the moisture content of banana flowers and Yunnan large-leaf sun-dried green tea reaches 60-70%.

[0035] During the blanching process, control the temperature at 200℃; knead until the strips are tightly curled and a little juice seeps out.

[0036] The sun-drying process involves spreading the kneaded banana flowers and Yunnan large-leaf sun-dried green tea leaves 3-5 cm apart and drying them in a sunny and well-ventilated area until the moisture content drops below 10%.

[0037] (2) Take the sun-dried banana flowers and Yunnan large-leaf sun-dried green tea, mix them evenly to obtain a mixture; place the mixture in a room for fermentation for 50 days; during the fermentation process, control the room temperature at 35℃ and the humidity at 70-85%; enter the room to turn the mixture 3 times a day.

[0038] (3) Add water to the camellia oil extract to make a suspension, add it to the mixture and mix evenly to make a mixture. Then dry it to a moisture content of 25%, and store it in a sealed room away from light for 2 days. During the sealed storage of the mixture, control the temperature in the room to be 25-35℃ and the humidity to be 70-80%. The height of the mixture during the stacking process is 50-70 cm. Enter the room and turn the mixture once every 2 days. After turning the mixture, continue to seal the room.

[0039] (4) After sealing and preservation, dry it by drying until the moisture content is no more than 14% and the drying temperature is no more than 60℃; then add rose petals, mix evenly, and place it in a well-ventilated and dry environment to age for 40 days to obtain the product. Example 3

[0040] A composition having AGEs inhibitory activity comprises the following parts by weight: 5 parts of camellia oil extract, 20 parts of banana flower, 15 parts of Yunnan large-leaf sun-dried green tea, and 10 parts of rose.

[0041] (1) Take freshly picked banana flowers and Yunnan large-leaf sun-dried green tea respectively, remove impurities, wash and dry them, then cut (cut or tear) the banana flowers into thin strips 0.5-1.0cm wide, and then wither, fix, roll and sun-dry them respectively for later use.

[0042] The withering process is as follows: banana flowers and Yunnan large-leaf sun-dried green tea are spread out on bamboo mats or bamboo sieves and placed in a well-ventilated, shaded indoor or outdoor place to wither until the moisture content of banana flowers and Yunnan large-leaf sun-dried green tea reaches 60-70%.

[0043] During the blanching process, control the temperature at 220℃; knead until the strips are tightly curled and a little juice seeps out.

[0044] The sun-drying process involves spreading the kneaded banana flowers and Yunnan large-leaf sun-dried green tea leaves 3-5 cm apart and drying them in a sunny and well-ventilated area until the moisture content drops below 10%.

[0045] (2) Take the sun-dried banana flowers and Yunnan large-leaf sun-dried green tea, mix them evenly to obtain a mixture; place the mixture in a room for fermentation for 60 days; during the fermentation process, control the room temperature at 40℃ and the humidity at 70-85%; enter the room to turn the material 3 times a day.

[0046] (3) Add water to the camellia oil extract to make a suspension, add it to the mixture and mix evenly to make a mixture. Then dry it to a moisture content of 30%, and store it in a sealed room away from light for 3 days. During the sealed storage of the mixture, control the temperature in the room to be 25-35℃ and the humidity to be 70-80%. The height of the mixture during the stacking process is 50-70 cm. Enter the room every 2 days to turn the mixture over. After turning the mixture over, continue to seal the room.

[0047] (4) After sealing and preservation, dry it by drying until the moisture content is no more than 14% and the drying temperature is no more than 60℃; then add rose petals, mix evenly, and place it in a well-ventilated and dry environment to age for 60 days to obtain the product.

[0048] In Examples 1-3, the banana flowers are freshly picked banana flowers, and the rose flowers are dried rose petals.

[0049] The preparation method of the camellia oil extract includes the following steps:

[0050] Camellia seed cake is defatted with petroleum ether at a material-to-liquid ratio of 1:8; the filtrate is removed by filtration, and the solid portion is dried in an electric heating oven at 50-60℃ to obtain defatted cake. Then, 5-6 times the weight of the defatted cake is added to water and heated and boiled for 20-30 minutes to remove insoluble matter, obtaining a filtrate. The filtrate is pre-cooled and then freeze-dried under vacuum to obtain the final product. The petroleum ether used must comply with the "National Food Safety Standard for Food Additives Petroleum Ether" (GB 1886.59-2015).

[0051] The compositions prepared in the above embodiments have the activity of inhibiting advanced glycation end products (AGEs) and can be used to prepare formulations that inhibit AGEs, especially suitable for the preparation of tea bags.

[0052] The classic protein glycosylation model (BSA-FRU) was selected for activity assay. The reaction system contained 60 mg / mL BSA and 1.5 M FRU, prepared in 50 mM PBS (containing 0.02% NaN3, pH 7.4). Each sample was added at a dose of 3.0 mg / mL (calculated based on the amount of the prepared composition). Each sample was diluted with six times its volume of boiling water, and the filtrate was concentrated to an equal mass before being added. The tubes were incubated at 37°C for 7 days in an incubator (SPX-250B-Z, Shanghai Boxun, China). Fluorescence intensity was measured at excitation / emission wavelengths of 325 / 440 nm using a fluorescence spectrophotometer (Hitachi F-7000, Tokyo, Japan). Differences in AGEs were compared, and the inhibition rate of each fraction was calculated.

[0053] Inhibition rate (%) = (λ) 对照 -λ 样品 ) / λ 对照 ×100%

[0054] Where, λ 对照 λ represents the fluorescence intensity of the control group after culture. 对照 The fluorescence intensity is shown in the control group after culture.

[0055] The preparation methods for each sample to be tested are shown in Table 1.

[0056] Sample 1: The composition was prepared according to the proportions and preparation method of Example 1.

[0057] Sample 2: The composition was prepared according to the proportions and preparation method of Example 2.

[0058] Sample 3: The composition was prepared according to the proportions and preparation method described in Example 3.

[0059] Sample 4: The material ratio and preparation method of Example 2 were followed, but banana flowers were not added.

[0060] Sample 5: Following the material ratio and preparation method of Example 2, without adding Yunnan large-leaf sun-dried green tea.

[0061] Sample 6: Refer to the material ratio and preparation method of Example 2, but do not add tea oil extract in step (3), and the rest of the operation remains unchanged.

[0062] Sample 7: The material ratio of Example 2 is referenced, but in the preparation process, banana flowers, Yunnan large-leaf sun-dried green tea and tea oil extract are added at once in step (2), dried to a moisture content of 10%, and then composted for 52 days, and then processed according to the method of step (4).

[0063] The AGEs inhibition rates of each sample are shown in Table 1.

[0064] Table 1 Comparison of AGEs inhibition rates among different samples

[0065] Sample No. Inhibition Rate (%) Sample 1 21.15 Sample 2 22.34 Sample 3 20.48 Sample 4 13.53 Sample 5 10.48 Sample 6 14.60 Sample 7 11.12

[0066] As shown in Table 1, all seven tested samples exhibited some AGEs inhibitory activity. Samples 1-3 showed the strongest inhibitory activity. For samples 4-6, although the material ratio was changed and the preparation method was the same as in Example 2, the AGEs inhibitory activity was still reduced to varying degrees compared to samples 1-3. Sample 7, although using the same material ratio as in Example 2, underwent a one-time sealed fermentation process, resulting in an improved AGEs inhibition rate compared to sample 5, but still lower than samples 4 and 6, and significantly lower than samples 1-3. These experimental results demonstrate that the method of first sealing and fermenting banana flowers, Yunnan large-leaf sun-dried green tea, and rose flowers, followed by adding camellia oil extract for further fermentation, can significantly improve the AGEs content of the prepared composition. Changing the material composition or the fermentation method significantly affects the AGEs inhibitory activity.

[0067] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A composition having AGEs inhibitory activity, characterized in that, The product contains the following components by weight: 1-5 parts of camellia oil extract, 10-20 parts of banana flower, 5-15 parts of Yunnan large-leaf sun-dried green tea, and 5-10 parts of rose.

2. The composition according to claim 1, characterized in that, The product contains the following components by weight: 3 parts camellia oil extract, 15 parts banana flower, 10 parts Yunnan large-leaf sun-dried green tea, and 8 parts rose.

3. The composition according to claim 1, characterized in that, The banana flowers mentioned are freshly picked banana flowers, and the rose flowers mentioned are dried rose flowers.

4. The composition according to claim 1, characterized in that, The preparation method of the camellia oil extract includes the following steps: Camellia seed cake is defatted and dried to obtain defatted cake. Then, 5-6 times the weight of the defatted cake is added to water and heated and boiled for 20-30 minutes to remove insoluble matter and obtain filtrate. The filtrate is then dried to obtain the final product.

5. A method for preparing a composition with AGEs inhibitory activity as described in any one of claims 1-4, characterized in that, Includes the following steps: Freshly picked banana flowers and Yunnan large-leaf sun-dried green tea leaves were taken separately. After removing impurities, washing and drying, the banana flowers were cut into thin strips. Then, they were withered, fixed, rolled and sun-dried separately for later use. Take sun-dried banana flowers and Yunnan large-leaf sun-dried green tea, mix them evenly to obtain a mixture; place the mixture in a room to ferment for 40-60 days. The camellia oil extract is mixed with water to form a suspension, which is then added to the mixture and mixed evenly to form a mixture. After drying to a moisture content of 20-30%, it is stored in a sealed room away from light for 1-3 days. After sealing and storing, dry it; then add dried rose petals, mix well, and age for at least 30 days to obtain the final product.

6. The preparation method according to claim 5, characterized in that, In step (1), the banana flowers are cut or torn into thin strips 0.5-1.0cm wide; the banana flowers and Yunnan large-leaf sun-dried green tea are spread on bamboo mats or bamboo sieves and placed in a well-ventilated indoor or outdoor shady place to wither until the moisture content of the banana flowers and Yunnan large-leaf sun-dried green tea reaches 60-70%; the temperature is controlled at 180-220℃ during the fixation process; the tea is kneaded until the strips are tightly rolled and a little juice seeps out; the sun-drying operation is as follows: spread the kneaded banana flowers and Yunnan large-leaf sun-dried green tea 3-5 cm apart and choose a sunny and well-ventilated place to dry until the moisture content drops to below 10%.

7. The preparation method according to claim 5, characterized in that, In step (2), during the fermentation of the mixture, the temperature in the room is controlled at 30-40℃ and the humidity at 70-85%; the material is turned over in the room 2-3 times a day; in step (3), during the sealed storage of the mixture, the temperature in the room is controlled at 30-40℃ and the humidity at 70-85%, the height of the mixture is 50-70 cm during the stacking process, the material is turned over in the room once every 2 days, and the room is sealed again after the material is turned over.

8. The preparation method according to claim 5, characterized in that, In step (4), the mixture is dried to a moisture content of no more than 14%; the drying is carried out by baking; the baking temperature does not exceed 60°C.

9. Use of the composition according to any one of claims 1-4 in the preparation of formulations that inhibit advanced glycation end products.

10. An formulation capable of inhibiting advanced glycation end products, comprising the composition according to any one of claims 1-4.