Stirring device for dsrna nano-nucleic acid insecticide for citrus psyllid

Abstract

The present application belongs to the technical field of magnetic stirring apparatuses. Disclosed is a stirring device for a dsRNA nano-nucleic acid insecticide for citrus psyllid, said stirring device comprising a base, wherein a gradual-separating lifting device is disposed at the top center of the base, the gradual-separating lifting device being provided with two vertical lifting ends, and an oscillation stirring device and a quick-release magnetic sleeve rack are fixedly mounted on the two vertical lifting ends in sequence from top to bottom, the oscillation stirring device comprising a plurality of coil oscillation structures arranged in a rectangular array.

Description

A stirring device for dsRNA nanonucleic acid insecticide for citrus psyllids Technical Field

[0001] This application relates to the field of magnetic stirring equipment technology, and in particular to a stirring device for a dsRNA nanonucleic acid insecticide for citrus psyllids. Background Technology

[0002] Magnetic bead extraction is a relatively mature method for nucleic acid extraction. Magnetic beads are supermagnetic particles that have a certain adsorption capacity for nucleic acids, while proteins and other impurities are not adsorbed and remain in the solution. Under the action of a magnetic field, the magnetic beads separate from the liquid, and the magnetic beads are recovered to obtain a magnetic bead-nucleic acid mixture. Finally, the nucleic acid can be obtained by elution.

[0003] After nucleic acid lysis, the magnetic rod, magnetic rod sleeve, and magnetic beads in the lysis buffer need to be separated. In some cases, the commonly used technical structure is a distributed multi-control structure, which requires multiple drive devices to separate the above components one by one and then clean or replace them in turn. This makes the structure of such magnetic bead nucleic acid extraction stirring device complex, occupies a large space, and results in cumbersome extraction operation steps, high equipment manufacturing costs, and limited compatibility between many components, which can easily cause equipment damage and reduce service life.

[0004] Secondly, the devices known to the applicant are mostly purely mechanical transmission structures, which generate significant noise during operation. This could easily affect the hearing of operators and is also detrimental to maintaining a good working environment.

[0005] Application content

[0006] In order to solve the above-mentioned problems in the prior art, the purpose of this application is to provide a stirring device for dsRNA nanonucleic acid insecticide for citrus psyllids.

[0007] The technical solution adopted in this application is as follows:

[0008] A stirring device for a dsRNA nano-nucleic acid insecticide for citrus psyllids includes a base. A gradually opening lifting device is located at the center of the top of the base. The gradually opening lifting device has two vertical lifting ends. A oscillating stirring device and a quick-release magnetic sleeve frame are fixedly installed on the two vertical lifting ends from top to bottom. The oscillating stirring device includes multiple coil oscillation structures arranged in a rectangular array. The quick-release magnetic sleeve frame has multiple oscillating magnetic sleeves arranged in a detachable rectangular array. Each coil oscillating structure corresponds one-to-one with each oscillating magnetic sleeve. A magnetic rod is fixedly installed at the bottom of each coil oscillating structure. Quick-clamping arms are provided on the left and right sides of the top of the base. The two quick-clamping arms are symmetrically arranged with the center of the base as the origin. A test tube tray is detachably clamped and positioned between the two quick-clamping arms. A test tube is provided on the surface of the test tube tray corresponding to each oscillating magnetic sleeve. The magnetic rod and the oscillating magnetic sleeve are driven synchronously towards or away from the corresponding test tube by the gradually opening lifting device. The magnetic rod is inserted into the oscillating magnetic sleeve, and the oscillating magnetic sleeve is inserted into the test tube. Attached Figure Description

[0009] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings of the embodiments of this application will be briefly described below. Obviously, the drawings described below only relate to some embodiments of this application and are not intended to limit this application. Wherein:

[0010] Figure 1 is a schematic diagram of the overall three-dimensional structure of one or more embodiments of this application;

[0011] Figure 2 is a three-dimensional exploded structure diagram of one or more embodiments of this application;

[0012] Figure 3 is an enlarged structural diagram of point A in Figure 2;

[0013] Figure 4 is an enlarged structural diagram of point B in Figure 2;

[0014] Figure 5 is a three-dimensional exploded structural diagram of one or more embodiments of the vibrating stirring device;

[0015] Figure 6 is a three-dimensional exploded structure schematic diagram of one or more embodiments of the coil oscillation structure;

[0016] The components include: 1. Base; 2. Involute lifting device; 21. Mounting bracket; 22. Electric telescopic rod; 23. Linear guide rail; 24. Slider; 25. First ear plate; 26. Scissor telescopic frame; 27. Second ear plate; 3. Quick clamp arm; 31. Column; 32. Snap-fit ​​groove; 33. Positioning post; 34. Pressing port; 35. Spring pin; 4. Test tube holder; 41. Positioning hole; 5. Test tube; 6. Quick-release magnetic sleeve frame; 61. Quick-release plate; 62. Outer frame; 63. L-shaped groove; 64. Locking pin; 65. Strong magnet; 7. Vibrating magnetic sleeve; 8. Vibrating stirring device; 81. Top cover; 82. Coil oscillation structure; 821. Vibrating rod core; 822. Coil assembly; 83. Circuit board; 84. Magnetic rod; 85. Mounting housing; 9. Controller. Detailed Implementation

[0017] To enable those skilled in the art to better understand this application, the application will be further described in detail below with reference to specific embodiments and accompanying drawings.

[0018] Example:

[0019] As shown in Figures 1-6, a stirring device for a dsRNA nano-nucleic acid insecticide for citrus psyllids includes a base 1. A gradually opening lifting device 2 is located at the center of the top of the base 1. The gradually opening lifting device 2 has two vertical lifting ends. A vibrating stirring device 8 and a quick-release magnetic sleeve frame 6 are fixedly installed on the two vertical lifting ends from top to bottom. The vibrating stirring device 8 includes multiple coil oscillation structures 82 arranged in a rectangular array. The quick-release magnetic sleeve frame 6 has multiple detachable rectangular arrays of oscillating magnetic sleeves 7. Each coil oscillation structure 82 is connected to each oscillating magnetic sleeve 7. In a corresponding manner, a magnetic rod 84 is fixedly installed at the bottom of the coil oscillation structure 82; quick-clamping arms 3 are provided on the top left and right sides of the base 1, and the two quick-clamping arms 3 are symmetrically arranged with the center of the base 1 as the origin. A test tube tray 4 is detachably clamped and positioned between the two quick-clamping arms 3. A test tube 5 is provided on the surface of the test tube tray 4 corresponding to each oscillating magnetic sleeve 7. The magnetic rod 84 and the oscillating magnetic sleeve 7 are driven synchronously to move closer to or away from the corresponding test tube 5 by the involute lifting device 2. The magnetic rod 84 is inserted into the oscillating magnetic sleeve 7, and the oscillating magnetic sleeve 7 is inserted into the test tube 5.

[0020] Specifically, as shown in Figure 1-2, by setting up an involute lifting device 2, and by driving each slider 24 to move synchronously closer or further away on the linear guide rail 23, the vibration stirring device 8 and the quick-release magnetic sleeve frame 6 are driven to move synchronously closer or further away from the test tube tray 4. This enables the magnetic rod 84 to be inserted into the vibration magnetic sleeve 7 and the vibration magnetic sleeve 7 to be inserted into the test tube 5 from top to bottom. At the same time, the involute action of the involute lifting device 2 increases the distance between the magnetic rod 84, the vibration magnetic sleeve 7 and the test tube 5, thus providing sufficient space for easy replacement of the vibration magnetic sleeve 7 and the test tube 5. The process is fully automatic and there is no structural interference, which is beneficial to improving the performance of the equipment.

[0021] Meanwhile, by symmetrically arranging two quick-clamping arms 3 on both sides of the base 1, the test tube tray 4 can be quickly clamped and replaced, thereby effectively improving the production efficiency of this stirring device.

[0022] Furthermore, to enhance the customizable mixing capabilities of this mixing device, it is achieved by incorporating a quick-release magnetic sleeve frame 6 and multiple coil oscillation structures 82 within the oscillating mixing device 8. Several oscillating magnetic sleeves 7 are integrated onto a quick-release plate 61 as a module. The modules are quickly assembled and disassembled with the outer frame 62, enabling the replacement of the oscillating magnetic sleeves 7 at any time. This allows for rapid replacement of the oscillating magnetic sleeves 7 after a batch of test tubes 5 has been mixed, facilitating the next mixing operation and resulting in high production performance. Combined with the coil oscillation structures 82, each coil oscillation structure 82 is connected via a circuit board and can be controlled by a controller 9 to achieve independent vibration with adjustable frequency. This allows the mixing device to handle multiple different products simultaneously, enabling the appropriate distribution of different liquids to be mixed, significantly improving the mixing effect and making the device more powerful and better suited to the user's production needs.

[0023] Secondly, since the vibration unit of this mixing equipment utilizes an electromagnetic coil, the equipment noise is significantly reduced, thereby effectively avoiding the harm caused to operators by working noise. Due to the reduction of friction, the service life of the equipment is effectively extended, making this mixing device more durable and economically viable.

[0024] The vibrating stirring device 8 includes a mounting housing 85, and multiple coil oscillation structures 82 are disposed inside the mounting housing 85. A top cover 81 is provided on the top of the mounting housing 85. The mounting housing 85 and the top cover 81 are provided with hollow holes corresponding to each coil oscillation structure 82. The magnetic rod 84 passes through the hollow holes and protrudes from the bottom of the mounting housing 85.

[0025] The coil oscillation structure 82 includes a coil assembly 822, multiple coil assemblies 822 are evenly soldered around the circuit board 83, a vibrating rod core 821 is movably connected to the center of the coil assembly 822, and a magnetic rod 84 is detachably fixedly installed at the bottom of the vibrating rod core 821.

[0026] Specifically, as shown in Figure 6, a magnetic rod 84 is installed at the bottom of the vibrating rod core 821 via a thread or other detachable connection method, forming the main power unit of this stirring device. This facilitates replacement and cleaning, and each unit can move synchronously and operate independently, allowing for highly customizable operation and further enhancing the practicality and versatility of this stirring device.

[0027] The quick-release magnetic sleeve 6 includes an outer frame 62. Multiple L-shaped slots 63 are equidistantly provided on the front and rear sides of the outer frame 62 along the length direction. A quick-release plate 61 is inserted between two opposite L-shaped slots 63. The end of the quick-release plate 61 is inserted into the transverse slot of the L-shaped slot 63. A locking pin 64 is provided at the top of the transverse slot. A strong magnet 65 is embedded at the bottom of the inner wall of the transverse slot corresponding to the locking pin 64. The bottom end of the locking pin 64 passes through multiple outer frames 62 and quick-release plates 61 in sequence and is magnetically attracted to the strong magnet 65. The vibrating magnetic sleeve 7 is fixedly connected to the top of the quick-release plate 61.

[0028] Specifically, as shown in Figures 2 and 4, multiple L-shaped grooves 63 are equidistantly opened on the two longer sides of the outer frame 62. After the two ends of the quick-release plate 61 are inserted through the vertical grooves of the corresponding two L-shaped grooves 63, the vertical freedom is restricted by sliding and locking it into the horizontal groove of the L-shaped groove 63. At the same time, to restrict the horizontal and vertical freedom of the quick-release plate 61, a locking pin 64 is detachably inserted into the horizontal slot of the L-shaped groove 63 at the top of the outer frame 62, and a strong magnet 65 is embedded in the corresponding position in the horizontal groove. A magnetic component is set at the bottom of the locking pin 64 to attract the strong magnet 65. When the end of the quick-release plate 61 is bolted by the locking pin 64, the quick-release plate 61 can be fixed. The above structure can realize the function of quickly replacing a set of vibrating magnetic sleeves 7. Thus, after the operation of this stirring device is completed, the vibrating magnetic sleeves 7 can be quickly replaced for the next operation, thereby preventing the vibrating magnetic sleeves 7 from carrying residual liquid and contaminating the stirring liquid of another operation.

[0029] The involute lifting device 2 includes a linear guide rail 23, a scissor-type telescopic frame 26, and a linear drive device. The linear guide rail 23 is fixedly installed in the center of the base 1 in the vertical direction. Two sliders 24 are slidably connected to the surface of the linear guide rail 23. The two sliders 24 are respectively fixedly installed with a second ear plate 27 and a first ear plate 25 from top to bottom. The second ear plate 27 and the first ear plate 25 are respectively fixedly installed with the vibrating stirring device 8 and the quick-release magnetic sleeve 6. The linear drive device is fixedly installed with the base 1 through the mounting bracket 21. The three hinge points of the scissor-type telescopic frame 26 on the same vertical side are respectively hinged to the second ear plate 27, the first ear plate 25, and the mounting bracket 21 from top to bottom. The lowest hinge point on the other vertical side of the scissor-type telescopic frame 26 is hinged to the telescopic end of the linear drive device.

[0030] Specifically, as shown in Figures 1-2, the linear drive device is an electric telescopic rod 22, which is fixedly installed on the top of the mounting bracket 21. Driven by the electric telescopic rod 22, the scissor telescopic frame 26 is driven to achieve telescopic movement. Since the movement characteristics of each connecting joint of the scissor telescopic frame 26 are synchronous and equidistant, the quick-release magnetic sleeve frame 6 and the oscillating stirring device 8 are respectively installed through the first ear plate 25 and the second ear plate 27, thereby realizing the insertion and withdrawal of the corresponding magnetic rod 84 and the oscillating magnetic sleeve 7, thus realizing the open and closed state of this stirring device, providing the operator with sufficient operating space.

[0031] The quick-clamping arm 3 includes a column 31, which is fixedly installed on one side of the top of the base 1. A snap-fit ​​groove 32 is fixedly installed on the top of the column 31. The test tube tray 4 is inserted between the two snap-fit ​​grooves 32. A limit structure is provided in the snap-fit ​​groove 32. The test tube tray 4 is locked with the limit structure by sliding into the snap-fit ​​groove 32. The test tube tray 4 is unlocked by pressing and dragging the limit structure.

[0032] The limiting structure includes two positioning posts 33, which are fixedly connected to the two ends of the top of the inner wall of the snap-fit ​​groove 32. Positioning holes 41 are opened at the four corners of the test tube tray 4 and at the corresponding positioning posts 33. The positioning posts 33 are inserted into the positioning holes 41. A spring pin 35 is fixedly installed in the center of the bottom of the snap-fit ​​groove 32. The top of the telescopic rod of the spring pin 35 passes through the inside of the snap-fit ​​groove 32 and is close to the bottom surface of the test tube tray 4.

[0033] A pressing opening 34 is provided in the center of one side of the top of the snap-fit ​​groove 32. The pressing opening 34 is arc-shaped.

[0034] Specifically, as shown in Figure 3, by inserting the side of the test tube tray 4 into the snap-fit ​​groove 32, the operator pushes it forward and presses it down lightly. The spring pin 35 is pressed back into the seat by the plate body, so that the test tube tray 4 can be pushed into place smoothly. After that, it is released, and the spring pin 35 rebounds and pushes the test tube tray 4 upward, so that the positioning holes 41 at the four corners are inserted and limited with the corresponding positioning posts 33, thereby realizing the quick installation and positioning of the test tube tray 4.

[0035] When it is necessary to disassemble the test tube tray 4, the operator presses down on the plate body at the pressing port 34, so that the positioning hole 41 separates from the positioning post 33, and then the test tube tray 4 can be pulled out to achieve disassembly. The operation is simple and convenient, which simplifies the installation process of the test tube 5 and improves production efficiency.

[0036] The interior of the mounting housing 85 is filled with sealant, which completely seals the circuit board 83. Through the encapsulation process, the waterproof performance of this device can be effectively improved, so that the coil oscillation structure 82 will not have the risk of leakage when energized, protecting the equipment so that it can be used normally and thus extending the service life of the equipment.

[0037] It should be noted that the electrical control principle of the coil oscillation structure 82 is a conventional circuit control principle, so it will not be elaborated here. For details on its specific circuit and control method, please refer to common knowledge and existing technology.

[0038] This application designs an involute lifting device 2, which is driven by an electric telescopic rod 22 to vertically extend and retract a scissor-type telescopic frame 26. Utilizing the characteristics of the scissor-type telescopic frame 26, it achieves synchronous movement of the quick-release magnetic sleeve frame 6 and the vibrating stirring device 8, thus simplifying the transmission structure and reducing equipment manufacturing costs. Simultaneously, it creatively utilizes an electromagnetic induction coil as the power source for vibration stirring, enabling each magnetic rod to function as an independent stirring unit. This allows each coil oscillation structure 82 to be controllable and adjustable, resulting in a high degree of parameter customization capability. Furthermore, compared to a purely mechanical transmission structure, it significantly reduces equipment operating noise and extends equipment lifespan, making the device more practical and economical.

[0039] This application, by setting up a quick-release magnetic sleeve holder 6 and two sets of quick-clamping arms 3, makes it easier and more convenient to replace the magnetic sleeve and test tube 5, thereby further improving the production efficiency and flexibility of the device.

[0040] This application is not limited to the above-mentioned optional embodiments. Anyone can derive other forms of products under the guidance of this application. However, regardless of any changes made in their shape or structure, any technical solution that falls within the scope of the claims of this application shall be protected by this application.

Claims

1. A stirring device for a dsRNA nanonucleic acid insecticide for citrus psyllids, comprising a base (1), characterized in that: The base (1) has an involute lifting device (2) at the top center. The involute lifting device (2) has two vertical lifting ends. The two vertical lifting ends are fixedly installed with a vibrating stirring device (8) and a quick-release magnetic sleeve (6) from top to bottom. The oscillating stirring device (8) includes multiple coil oscillating structures (82) arranged in a rectangular array. The quick-release magnetic sleeve frame (6) is provided with multiple oscillating magnetic sleeves (7) in a detachable rectangular array. Each coil oscillating structure (82) corresponds to each oscillating magnetic sleeve (7). A magnetic rod (84) is fixedly installed at the bottom of each coil oscillating structure (82). The base (1) is provided with quick clamping arms (3) on the top left and right sides. The two quick clamping arms (3) are symmetrically arranged with the center of the base (1) as the origin. A test tube tray (4) is detachably clamped and positioned between the two quick clamping arms (3). Test tubes (5) are provided on the surface of the test tube tray (4) corresponding to each of the oscillating magnetic sleeves (7). The magnetic rod (84) and the oscillating magnetic sleeve (7) are driven synchronously to move closer to or away from the corresponding test tubes (5) by the involute lifting device (2). The magnetic rod (84) is inserted into the oscillating magnetic sleeve (7), and the oscillating magnetic sleeve (7) is inserted into the test tube (5).

2. The stirring device for the dsRNA nanonucleic acid insecticide for citrus psyllids according to claim 1, characterized in that: The oscillating stirring device (8) includes a mounting housing (85), and a plurality of coil oscillation structures (82) are disposed inside the mounting housing (85). A top cover (81) is provided on the top of the mounting housing (85). The mounting housing (85) and the top cover (81) are provided with perforated holes corresponding to each coil oscillation structure (82). The magnetic rod (84) passes through the perforated holes and protrudes from the bottom of the mounting housing (85).

3. The stirring device for the dsRNA nanonucleic acid insecticide for citrus psyllids according to claim 2, characterized in that: The coil oscillation structure (82) includes a coil assembly (822), and multiple coil assemblies (822) are uniformly welded around the circuit board (83). A vibration rod core (821) is movably connected to the center of the coil assembly (822), and the magnetic rod (84) is detachably fixedly installed at the bottom of the vibration rod core (821).

4. The stirring device for the dsRNA nanonucleic acid insecticide for citrus psyllids according to claim 2, characterized in that: The quick-release magnetic sleeve frame (6) includes an outer frame (62). Multiple L-shaped slots (63) are equidistantly provided on the front and rear sides of the outer frame (62) along the length direction. A quick-release plate (61) is inserted between two opposite L-shaped slots (63). The end of the quick-release plate (61) is inserted into the transverse slot of the L-shaped slot (63). A locking pin (64) is provided at the top of the transverse slot. A strong magnet (65) is embedded at the bottom of the inner wall of the transverse slot corresponding to the locking pin (64). The bottom end of the locking pin (64) passes through the outer frame (62) and the quick-release plate (61) in sequence and magnetically attracts the strong magnet (65). The vibrating magnetic sleeve (7) is fixedly connected to the top of the quick-release plate (61).

5. The stirring device for the dsRNA nanonucleic acid insecticide for citrus psyllids according to claim 4, characterized in that: The involute lifting device (2) includes a linear guide rail (23), a scissor telescopic frame (26), and a linear drive device. The linear guide rail (23) is fixedly installed in the center of the base (1) in the vertical direction. Two sliders (24) are slidably connected to the surface of the linear guide rail (23). The two sliders (24) are respectively fixedly installed with a second ear plate (27) and a first ear plate (25) from top to bottom. The second ear plate (27) and the first ear plate (25) are respectively fixedly installed with the vibration stirring device (8) and the quick-release magnetic sleeve frame (6). The linear drive device is fixedly installed with the base (1) through a mounting bracket (21). The three hinge points of the scissor telescopic frame (26) on the same vertical side are respectively hinged to the second ear plate (27), the first ear plate (25), and the mounting bracket (21) from top to bottom. The lowest hinge point on the other vertical side of the scissor telescopic frame (26) is hinged to the telescopic end of the linear drive device.

6. The stirring device for the dsRNA nanonucleic acid insecticide for citrus psyllids according to claim 5, characterized in that: The linear drive device is an electric telescopic rod (22), which is fixedly installed on the top of the mounting bracket (21).

7. The stirring device for the dsRNA nanonucleic acid insecticide for citrus psyllids according to claim 1, characterized in that: The quick clamp arm (3) includes a column (31), which is fixedly installed on one side of the top of the base (1). A snap-fit ​​groove (32) is fixedly installed on the top of the column (31). The test tube tray (4) is inserted between the two snap-fit ​​grooves (32). A limiting structure is provided in the snap-fit ​​groove (32). The test tube tray (4) is locked with the limiting structure by sliding into the snap-fit ​​groove (32). The test tube tray (4) is unlocked by pressing and dragging the limiting structure.

8. The stirring device for the dsRNA nanonucleic acid insecticide for citrus psyllids according to claim 7, characterized in that: The limiting structure includes two positioning posts (33), which are fixedly connected to the two ends of the top of the inner wall of the snap-fit ​​groove (32). The four corners of the test tube tray (4) and the corresponding positioning posts (33) are provided with positioning holes (41). The positioning posts (33) are inserted into the positioning holes (41). A spring pin (35) is fixedly installed in the center of the bottom of the snap-fit ​​groove (32). The top of the telescopic rod of the spring pin (35) passes through the inside of the snap-fit ​​groove (32) and is close to the bottom surface of the test tube tray (4).

9. The stirring device for the dsRNA nanonucleic acid insecticide for citrus psyllids according to claim 8, characterized in that: A pressing opening (34) is provided in the center of one side of the top of the snap-fit ​​groove (32), and the pressing opening (34) is arc-shaped.

10. The stirring device for the dsRNA nanonucleic acid insecticide for citrus psyllids according to claim 3, characterized in that: The interior of the mounting housing (85) is filled with sealant, which completely seals the circuit board (83).

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