Fully automatic nucleic acid methylation processing system

Abstract

Disclosed in the present application is a fully automatic nucleic acid processing system, comprising a control assembly, a detection cartridge carrier assembly, an extraction and purification pipetting assembly, a temperature control assembly, a driving assembly, and a fluorescence channel assembly. The control assembly separately controls the detection cartridge carrier assembly, the extraction and purification pipetting assembly, the temperature control assembly, the driving assembly, and the fluorescence channel assembly. The detection cartridge carrier assembly is used for placing a nucleic acid extraction and detection cartridge. The extraction and purification pipetting assembly is arranged above the detection cartridge carrier assembly in a liftable / lowerable manner, and is used for pipetting, oscillation, and / or magnetic attraction during the nucleic acid extraction process. The driving assembly is used for driving the detection cartridge carrier assembly to perform reciprocating motion in a first direction and driving the lifting and lowering of the extraction and purification pipetting assembly in a second direction. The temperature control assembly is arranged below a fixing sleeve, and is used for providing a temperature condition for the nucleic acid extraction and detection cartridge inside the fixing sleeve. The fluorescence channel assembly is used for performing PCR detection on a sample inside the nucleic acid extraction and detection cartridge.

Description

A fully automated nucleic acid methylation processing system Technical Field

[0001] This application relates to the field of molecular diagnostic technology, specifically to a fully automated nucleic acid methylation processing system. Background Technology

[0002] In the field of molecular biology research, nucleic acid extraction and PCR amplification detection are two core experimental techniques. With continuous advancements in technology, experimental science is undergoing a transformation from traditional manual operations to automated equipment. Automated equipment, with its superior repeatability and consistency, significantly reduces the cumulative errors caused by manual operations, greatly improving experimental efficiency and the accuracy of results.

[0003] DNA methylation is an important area of ​​research in epigenetics, and its alteration is considered a key factor in tumorigenesis. These alterations include a decrease in overall genome methylation levels and an abnormal increase in localized CpG island methylation levels. These changes can lead to genomic instability, thereby inducing cellular carcinogenesis. Compared to gene mutations, epigenetic variations are more frequent; therefore, utilizing these variations for tumor diagnosis can provide higher sensitivity and specificity. As an early event in tumorigenesis, DNA methylation detection for early tumor diagnosis has significant clinical value.

[0004] Circulating tumor DNA (ctDNA) is a DNA fragment derived from the tumor genome and carried in the human bloodstream. Its main sources are necrotic tumor cells, apoptotic tumor cells, circulating tumor cells, and efflux bodies secreted by tumor cells. This tumor DNA often contains methylation characteristics specific to the tumor genome.

[0005] Currently, the purification of cell-free DNA in plasma, sulfite treatment, and nucleic acid detection mostly employ a multi-platform, manual, and interconnected approach. This not only requires the use of multiple devices in conjunction but also necessitates multiple manual interventions, leading to inconsistent sample dwell times, prolonged testing times, poor sample repeatability, and increased cumulative errors across multiple platforms. The challenges of automating this process are: Challenge 1: Achieving end-to-end detection: How to implement end-to-end detection from sample processing to result output on a single device to eliminate or reduce cumulative errors across multiple platforms and devices, and minimize manual intervention. Challenge 2: Closed-loop nucleic acid detection: How to perform closed-loop nucleic acid detection within the device or its associated consumables to meet the requirements of a dedicated PCR testing environment. Challenge 3: Large-volume sample processing: Current nucleic acid testing primarily targets small-volume samples and lacks the capacity to process large-volume samples (such as 10ml mixtures). Summary of the Invention

[0006] To address the aforementioned deficiencies, this application provides a fully automated nucleic acid methylation processing system. The entire process, from the entry of a plasma sample into the device to obtaining the PCR detection result of the target DNA, is completed entirely by the device without human intervention, achieving a high degree of automation in nucleic acid methylation extraction and detection.

[0007] This application provides the following technical solution.

[0008] 1. A fully automated nucleic acid processing system, comprising a control component, a detection cartridge carrier component, an extraction and purification pipetting component, a temperature control component, a drive component, and a fluorescence channel component, wherein,

[0009] The control components control the detection kit carrier assembly, the extraction and purification pipetting assembly, the temperature control assembly, the drive assembly, and the fluorescence channel assembly, respectively.

[0010] The detection kit carrier assembly is used to hold the nucleic acid extraction detection kit;

[0011] The detection box carrier assembly includes a first fixing part and at least one set of heating fixing parts. The first fixing part includes a first fixing member and a second fixing member disposed opposite to each other. At least one set of heating fixing parts is disposed between the first fixing member and the second fixing member. Each set of heating fixing parts includes at least two spaced fixing sleeves. The first fixing member and the second fixing member are used to restrict the two ends of the nucleic acid extraction detection box, and the fixing sleeves are used to restrict a part of the nucleic acid extraction detection box.

[0012] The extraction and purification pipetting assembly is vertically and vertically positioned above the detection kit carrier assembly, and is used for pipetting, shaking, and / or magnetic suction during nucleic acid extraction.

[0013] The driving component is used to drive the detection box carrier assembly to reciprocate along the first direction and drive the extraction and purification pipetting assembly to move up and down along the second direction.

[0014] The temperature control component is located below the fixing sleeve and is used to provide temperature conditions for the nucleic acid extraction and detection box inside the fixing sleeve;

[0015] The fluorescence channel assembly is used for PCR detection of samples in the nucleic acid extraction and detection kit.

[0016] 2. The system according to claim 1, wherein the sidewall of the first fixing member facing the second fixing member has at least one set of first fixing grooves recessed therein.

[0017] The second fastener has at least one set of second fastening grooves recessed into its sidewall facing the first fastener.

[0018] 3. The system according to item 2, wherein a detection hole is further provided between the first fixing member and the second fixing member, the detection hole being located on the side close to the first fixing member, and the number of heating fixing parts and the number of detection holes are equal to the number of the first fixing groove and the second fixing groove.

[0019] 4. The system according to item 3, wherein the system further includes a first base plate, the driving component includes a first driving component, the temperature control component is disposed on the first base plate, the first driving component is used to drive the first base plate to reciprocate along a first direction, thereby driving the temperature control component and the detection box carrier assembly to reciprocate along the first direction.

[0020] 5. The system according to item 4, wherein the system further includes a second base plate, the first drive assembly is disposed on the second base plate, and the first base plate moves on the second base plate along a first direction.

[0021] 6. The system according to item 1, wherein the extraction and purification pipetting assembly includes a magnetic rod holder assembly and a multi-channel pipetting assembly, the magnetic rod holder assembly being vertically and vertically disposed above the detection kit carrier assembly, and is used for oscillation and / or magnetic attraction during the nucleic acid extraction process, the multi-channel pipetting assembly being vertically and vertically disposed above the detection kit carrier assembly, and is used to drive the syringe to pipette during the nucleic acid extraction process.

[0022] 7. The system according to item 6, wherein the system further includes a fourth base plate, a first bracket, a second bracket, a fourth bracket, and a fifth bracket, the fourth base plate being fixedly connected to the second base plate via a support column, and the first bracket, the second bracket, the fourth bracket, and the fifth bracket being connected to the fourth base plate.

[0023] 8. The system according to item 7, wherein the magnetic rod holder assembly includes a plurality of magnetic rods and magnetic rod sleeves arranged in an array, the magnetic rods being located above the magnetic rod sleeves and capable of moving downwards to extend into the magnetic rod sleeves.

[0024] 9. The system according to item 8, wherein the driving component further includes a second driving component and a third driving component, the second driving component being used to drive the magnetic rod to move up and down along a second direction, and the third driving component being used to drive the magnetic rod sleeve to move up and down along the second direction;

[0025] The magnetic rod holder assembly is located above the nucleic acid extraction and detection box. The second and third driving components drive the magnetic rod holder assembly or magnetic rod sleeve to move above the nucleic acid extraction and detection box, and can move downward to extend the magnetic rod holder assembly or magnetic rod sleeve into the nucleic acid extraction and detection box.

[0026] 10. The system according to item 8 or 9, wherein the first support is provided with m columns of magnetic bars, the spacing of the m columns of magnetic bars is adjustable, each column has n magnetic bars, and the spacing of the n magnetic bars is adjustable;

[0027] The second support is provided with m columns of magnetic rod sleeves, the spacing of the m columns of magnetic rod sleeves is adjustable, each column has n magnetic rod sleeves, and the spacing of the n magnetic rod sleeves is adjustable;

[0028] Where m≥1, n≥2.

[0029] 11. The system according to item 7, wherein the multi-channel pipetting assembly includes a syringe driving part and a piston driving part, a portion of the piston driving part is sleeved inside the syringe driving part, and the piston driving part is vertically and vertically disposed inside the syringe driving part along a second direction.

[0030] The syringe drive unit is used to drive the syringe syringe to move up and down in the second direction;

[0031] The piston drive unit is used to drive the piston of the syringe to move up and down in the second direction.

[0032] 12. The system according to item 11, wherein the driving assembly further includes a fourth driving assembly and a fifth driving assembly, the fourth driving assembly being used to drive the syringe driving part to move up and down along the second direction, and the fifth driving assembly being used to drive the piston driving part to move up and down along the second direction;

[0033] The multi-channel pipetting assembly is located above the nucleic acid extraction and detection box. The fourth and fifth driving components drive the multi-channel pipetting assembly or the syringe driving part to move above the nucleic acid extraction and detection box, and can move downward to allow the syringe to extend into the nucleic acid extraction and detection box.

[0034] 13. The system according to item 11 or 12, wherein the fourth support is provided with m columns of syringe driving units, the spacing between the m columns of syringe driving units is adjustable, each column has n syringe driving units, and the spacing between the n syringe driving units is adjustable, or

[0035] The fourth support is provided with an injection cylinder driving assembly, which has m columns of mutually spaced injection cylinder driving parts. The spacing between the m columns of injection cylinder driving parts is adjustable. Each column has n injection cylinder driving parts, and the spacing between the n injection cylinder driving parts is adjustable.

[0036] The fifth support is provided with m columns of piston drive units, the spacing between the m columns of piston drive units is adjustable, each column has n piston drive units, and the spacing between the n piston drive units is adjustable; where m≥1, n≥2.

[0037] 14. The system according to item 1, wherein the system further includes a third base plate disposed above the detection box carrier assembly, and the third base plate has an opening in the middle, through which the multi-channel pipetting assembly and the magnetic rod holder assembly pass when moving in the second direction.

[0038] 15. The system according to item 14, wherein the driving component further includes a sixth driving component, the sixth driving component being used to drive the third base plate to move up and down along a second direction.

[0039] 16. The system according to item 15, wherein a first pressure plate and a second pressure plate are provided on the side surface of the third base plate facing the detection box carrier assembly, the first pressure plate is located above the first fixing member, the second pressure plate is located above the second fixing member, and the first pressure plate and the second pressure plate are driven to descend in a second direction by the sixth driving assembly, so that the first pressure plate and the second pressure plate press on the top of the nucleic acid extraction detection box, thereby fixing the nucleic acid extraction detection box on the detection box carrier assembly.

[0040] 17. The system according to item 16, wherein the first pressure plate has a first slot on the side surface facing the nucleic acid extraction and detection box, and the second pressure plate has a second slot on the side surface facing the nucleic acid extraction and detection box, the first slot and the second slot being used to engage the nucleic acid extraction and detection box.

[0041] 18. The system according to item 4, wherein the fluorescence channel assembly is disposed on the first base plate, and the optical fiber of the fluorescence channel assembly extends into the detection well, thereby performing PCR detection on the sample in the nucleic acid extraction detection kit.

[0042] 19. The system according to item 1, wherein the temperature control component includes a heating unit and a cooling unit, the heating unit being disposed directly below the fixing sleeve, and the cooling unit being disposed on one side of the heating unit for cooling the heating unit.

[0043] 20. The system according to claim 1, wherein the system further includes a display mechanism connected to the control mechanism for issuing instructions to the control mechanism.

[0044] The fully automated nucleic acid methylation system described in this application fixes the nucleic acid extraction detection box using the detection box carrier assembly. Plasma samples are then added to the nucleic acid extraction detection box. A drive assembly moves the multi-channel pipetting component within the extraction and purification pipetting assembly, which in turn moves the syringe to transfer different reagents to corresponding positions within the nucleic acid extraction detection box. The drive assembly also moves the magnetic rod holder assembly to agitate and extract the sample solution within the nucleic acid extraction detection box. Because the syringe needs to transfer different reagents to different positions within the nucleic acid extraction detection box, the multi-channel pipetting component can only move up and down in a second direction. The drive assembly moves the detection box carrier assembly in a first direction, allowing the multi-channel pipetting component to move the syringe to different positions within the nucleic acid extraction detection box. After the plasma sample within the nucleic acid extraction detection box is processed, PCR detection is performed on the sample using the fluorescence channel assembly, thus completing the extraction and PCR detection of the plasma sample.

[0045] The fully automated nucleic acid methylation system described in this application completes the entire process from plasma sample entry into the device to obtaining the PCR detection result of the target DNA entirely by the equipment, without human intervention, achieving a high degree of automation in nucleic acid methylation extraction and detection. Furthermore, the various components in this application are highly independent, allowing for collaborative work between components while individual components can also perform their respective tasks; the relative positions of all components can be varied depending on space constraints, offering flexibility in their relative placement. Attached Figure Description

[0046] Figure 1 is a schematic diagram of the fully automated nucleic acid methylation processing system provided in this application.

[0047] Figure 2 is a schematic diagram of the fully automated nucleic acid methylation processing system provided in this application.

[0048] Figure 3 is a schematic diagram of the fully automated nucleic acid methylation processing system provided in this application.

[0049] Figure 4 is a schematic diagram of the fully automated nucleic acid methylation processing system provided in this application.

[0050] Figure 5 is a schematic diagram of the fully automated nucleic acid methylation processing system provided in this application.

[0051] Explanation of reference numerals in the attached drawings: 1-First base plate, 2-Second base plate, 3-Third base plate, 4-Fourth base plate, 5-First bracket, 6-Second bracket, 7-Fourth bracket, 8-First drive motor, 9-Fifth bracket, 10-Third drive motor, 11-Fourth drive motor, 12-Fifth drive motor, 13-Sixth drive motor, 14-Support column, 15-First fixing component, 16-Second fixing component, 17-First pressure plate, 18-Heating unit, 19-Cooling unit, 20-Second drive motor, 21-Piston drive section, 22-Injection cylinder drive section assembly, 23-Injection cylinder, 24-Magnetic rod holder assembly, 25-Fixing sleeve. Detailed Implementation

[0052] The following description provides exemplary embodiments of this application, including various details to aid understanding, and should be considered merely exemplary. Therefore, those skilled in the art will recognize that various changes and modifications can be made to the embodiments described herein without departing from the scope and spirit of this application. Similarly, for clarity and brevity, descriptions of well-known functions and structures are omitted in the following description.

[0053] As shown in Figures 1-5, this application provides a fully automated nucleic acid processing system, which includes a control component, a detection cartridge carrier component, an extraction and purification pipetting component, a temperature control component, a drive component, and a fluorescence channel component, wherein...

[0054] The control components control the detection kit carrier assembly, the extraction and purification pipetting assembly, the temperature control assembly, the drive assembly, and the fluorescence channel assembly, respectively.

[0055] The detection kit carrier assembly is used to place the nucleic acid extraction detection kit. In this document, the nucleic acid extraction detection kit can be a conventional multi-well plate (e.g., a 96-well plate or a 48-well plate) or a multi-well plate with a liquid storage function. A multi-well plate with a liquid storage function refers to a multi-well plate that includes liquid storage wells and experimental wells. The liquid storage wells store reagents and magnetic beads required for nucleic acid extraction and purification, while the experimental wells provide the space required for nucleic acid extraction and purification and other experimental steps.

[0056] The detection kit carrier assembly can hold multiple nucleic acid extraction detection kits, allowing for the simultaneous extraction of multiple samples.

[0057] The detection kit carrier assembly includes a first fixing part and at least one set of heating fixing parts. The first fixing part includes a first fixing member 15 and a second fixing member 16 disposed opposite to each other. At least one set of heating fixing parts is disposed between the first fixing member 15 and the second fixing member 16. Each set of heating fixing parts includes at least two spaced fixing sleeves 25. The first fixing member 15 and the second fixing member 16 are respectively used to restrict the two ends of the nucleic acid extraction detection kit. The fixing sleeves 25 are used to restrict a part of the nucleic acid extraction detection kit. When the nucleic acid extraction detection kit has multiple holes, some holes can be placed inside the fixing sleeves 25.

[0058] The height of the first fixing member 15 and the second fixing member 16 is higher than the height of the fixing sleeve 25.

[0059] The number of fixing sleeves 25 in each group of heating fixing parts can be 2, 3, 4, 5, 6, or more, and the number of fixing sleeves 25 can be determined according to actual needs. The shape and size of the fixing sleeves 25 in each group of heating fixing parts can be the same or different. For example, the shape of the fixing sleeve 25 can be a columnar structure or a strip-shaped groove structure, etc.

[0060] The number of heating fixing parts can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc., and the number of heating fixing parts can be determined according to actual needs.

[0061] The extraction and purification pipetting assembly is vertically and vertically positioned above the detection kit carrier assembly, and is used for pipetting, shaking, and / or magnetic suction during nucleic acid extraction.

[0062] Furthermore, the extraction and purification pipetting assembly includes a magnetic rod holder assembly 24 and a multi-channel pipetting assembly. The magnetic rod holder assembly 24 is vertically and vertically positioned above the detection kit carrier assembly and is used for oscillation and / or magnetic attraction during the nucleic acid extraction process. The multi-channel pipetting assembly is vertically and vertically positioned above the detection kit carrier assembly and is used to drive the syringe to pipette during the nucleic acid extraction process.

[0063] The driving component is used to drive the detection box carrier assembly to reciprocate along the first direction and drive the extraction and purification pipetting assembly to move up and down along the second direction.

[0064] Furthermore, the driving component is used to drive the detection box carrier assembly to reciprocate along the first direction, drive the magnetic rod holder assembly 24 to move up and down along the second direction, and drive the multi-channel pipetting assembly to move up and down along the second direction.

[0065] In this paper, the first direction is perpendicular to the second direction.

[0066] The temperature control component is located below the fixing sleeve 25 and is used to provide temperature conditions for the nucleic acid extraction and detection box inside the fixing sleeve 25;

[0067] The fluorescence channel assembly is used for PCR detection of samples in the nucleic acid extraction and detection kit.

[0068] The fully automated nucleic acid methylation system described in this application fixes the nucleic acid extraction detection box using the detection box carrier assembly. Plasma is then added to the nucleic acid extraction detection box. A drive assembly drives the multi-channel pipetting component in the extraction and purification pipetting assembly, which in turn drives the syringe to transfer different reagents to corresponding positions within the nucleic acid extraction detection box. The drive assembly also drives the magnetic rod holder assembly 24 to agitate and extract the sample solution within the nucleic acid extraction detection box. Because the syringe needs to transfer different reagents to different positions within the nucleic acid extraction detection box, the multi-channel pipetting component can only move up and down in a second direction. The drive assembly drives the detection box carrier assembly to move in a first direction, allowing the multi-channel pipetting component to drive the syringe to transfer different reagents to different positions within the nucleic acid extraction detection box. After the plasma sample within the nucleic acid extraction detection box is processed, PCR detection is performed on the sample using the fluorescence channel assembly, thus completing the extraction of the plasma sample and the PCR detection.

[0069] In this application, the sidewall of the first fixing member 15 facing the second fixing member 16 has at least one set of first fixing grooves recessed inward therein, and the sidewall of the second fixing member 16 facing the first fixing member 15 has at least one set of second fixing grooves recessed inward therein.

[0070] The two ends of the nucleic acid extraction and detection kit can be respectively engaged with the first fixing slot and the second fixing slot, thereby restricting the position of the nucleic acid extraction and detection kit and ensuring that the nucleic acid extraction and detection kit will not move during the nucleic acid extraction process, thus avoiding affecting the nucleic acid extraction process.

[0071] In some embodiments, when the nucleic acid extraction detection kit has a row of wells, the nucleic acid extraction detection kit is arranged along the direction from the first fixing member 15 to the second fixing member 16. With this design, the detection kit carrier assembly of this application can simultaneously fix multiple nucleic acid extraction detection kits, thereby enabling simultaneous nucleic acid extraction and purification of multiple samples.

[0072] Furthermore, a detection hole is provided between the first fixing member 15 and the second fixing member 16. The detection hole is located on the side closer to the first fixing member 15. The number of heating fixing parts and the number of detection holes are equal to the number of the first fixing groove and the second fixing groove.

[0073] Furthermore, in the direction from the first fixing member 15 to the second fixing member 16, a detection holes and a sets of heating fixing parts are sequentially arranged between the first fixing member 15 and the second fixing member 16. Each set of heating fixing parts includes b spaced-apart fixing sleeves 25. Each detection hole corresponds to a set of heating fixing parts, and the detection hole and its corresponding multiple fixing sleeves 25 are on the same straight line. Where a is greater than or equal to 1, and b is greater than or equal to 2.

[0074] In some embodiments, a detection hole and a set of heating fixing parts are sequentially provided between the first fixing member 15 and the second fixing member 16 in the direction from the first fixing member 15 to the second fixing member 16, and each set of heating fixing parts includes four fixing sleeves 25 arranged at intervals.

[0075] In some embodiments, two detection holes and two sets of heating fixing parts are sequentially arranged between the first fixing member 15 and the second fixing member 16 in the direction from the first fixing member 15 to the second fixing member 16, and each set of heating fixing parts includes four fixing sleeves 25 arranged at intervals.

[0076] In this application, the system further includes a first base plate 1, the driving component includes a first driving assembly, and the temperature control assembly is disposed on the first base plate 1. The first driving assembly is used to drive the first base plate 1 to reciprocate along a first direction, thereby driving the temperature control assembly and the detection box carrier assembly on the temperature control assembly to reciprocate along the first direction. When it is necessary to pipette or agitate at different positions within the nucleic acid extraction detection box, the first driving assembly drives the detection box carrier assembly and the nucleic acid extraction detection box thereon to move along the first direction.

[0077] The first direction is consistent with the direction from the first fastener 15 to the second fastener 16.

[0078] Furthermore, the system also includes a second base plate 2, on which the first drive component is disposed, and the first base plate 1 moves on the second base plate 2 along a first direction.

[0079] The first driving assembly includes a first driving motor 8, a first driving shaft, a first slider, and a first slide rail. The first slide rail is disposed on the second base plate 2, and the first slider is disposed on the bottom of the first base plate 1. The first driving motor 8 is connected to the first driving shaft, and the first driving shaft is connected to the slider. The first driving motor 8 can drive the first driving shaft to reciprocate along a first direction, thereby driving the first slider to reciprocate along the first direction on the first slide groove, and further driving the first base plate 1 and the various parts of the structure on the first base plate 1 to reciprocate along the first direction.

[0080] In this application, the system further includes a fourth base plate 4, a first support 5, a second support 6, a fifth support 9, and a fourth support 7. The fourth base plate 4 is fixedly connected to the second base plate 2 via a support column 14. The first support 5, the second support 6, the fifth support 9, and the fourth support 7 are all connected to the fourth base plate 4. The second base plate is placed on a table or the ground, and the fourth base plate is fixed to the second base plate via the support column. The first support, the second support, the fourth support, and the fifth support are directly or indirectly connected to the fourth base plate. This design allows for the vertical movement of the magnetic rod, magnetic rod sleeve, and pipetting assembly while maintaining a compact structure, resulting in a smaller device size and more flexible transmission.

[0081] The number of support columns 14 can be four, and the four support columns 14 support the fourth base plate 4 above the second base plate 2.

[0082] Furthermore, the magnetic rod holder assembly 24 includes a plurality of magnetic rods and magnetic rod sleeves arranged in an array, wherein the magnetic rods are located above the magnetic rod sleeves and can move downwards to extend into the magnetic rod sleeves.

[0083] Furthermore, the driving assembly also includes a second driving assembly and a third driving assembly, wherein the second driving assembly is used to drive the magnetic rod to move up and down along the second direction, and the third driving assembly is used to drive the magnetic rod sleeve to move up and down along the second direction;

[0084] The magnetic rod holder assembly 24 is located above the nucleic acid extraction and detection box. The second driving assembly and the third driving assembly drive the magnetic rod holder assembly 24 or the magnetic rod sleeve to move above the nucleic acid extraction and detection box, and can move downward to allow the magnetic rod holder assembly 24 or the magnetic rod sleeve to extend into the nucleic acid extraction and detection box.

[0085] The second drive assembly includes a second drive motor 20 and a second drive shaft. The second drive motor 20 is connected to the second drive shaft, and the second drive shaft is connected to the first bracket 5. Thus, when the second drive motor 20 drives the second drive shaft to move up and down in the second direction, the first bracket 5 will also move up and down in the second direction, thereby driving the magnetic rod to move up and down in the second direction.

[0086] The third drive assembly includes a third drive motor 10 and a third drive shaft. The third drive motor 10 is connected to the third drive shaft, and the third drive shaft is connected to the second bracket 6. Thus, when the third drive motor 10 drives the third drive shaft to move up and down in the second direction, the second bracket 6 will also move up and down in the second direction, thereby driving the magnetic rod sleeve to move up and down in the second direction.

[0087] Further, the first bracket 5 includes a first fixed bracket and a first connecting bracket. The first fixed bracket is connected to the second drive shaft, and the first connecting bracket is connected to the magnetic rod. The second bracket 6 includes a second fixed bracket and a second connecting bracket. The second fixed bracket is connected to the third drive shaft, and the second connecting bracket is connected to the magnetic rod sleeve. The first connecting bracket has a first through hole, and the second connecting bracket passes through the first through hole, so that the first connecting bracket is located above the second connecting bracket, thereby positioning the magnetic rod directly above the magnetic rod sleeve.

[0088] Furthermore, a first limiting cylinder is provided on the first connecting bracket surrounding the first through hole, and the second connecting bracket passes through the first limiting cylinder and the first through hole. When the second connecting bracket moves up and down, the first connecting bracket is not affected, and the first limiting cylinder can limit the offset or shaking of the second connecting bracket when it moves up and down.

[0089] Furthermore, the first support 5 is provided with m columns of magnetic bars, the spacing of the m columns of magnetic bars is adjustable, each column has n magnetic bars, and the spacing of the n magnetic bars is adjustable;

[0090] The second support 6 is provided with m columns of magnetic rod sleeves, the spacing of the m columns of magnetic rod sleeves is adjustable, each column has n magnetic rod sleeves, and the spacing of the n magnetic rod sleeves is adjustable;

[0091] Where m≥1, n≥2.

[0092] m can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc., and n can be 2, 3, 4, 5, 6, 7, 8, 9, 10, etc. The number of m and n can be designed according to actual needs.

[0093] The adjustable spacing between adjacent magnetic rods / magnetic rod sleeves can be achieved using a telescopic rod.

[0094] In some embodiments, the first support 5 is provided with a row of 12 magnetic rods, the 12 magnetic rods being equally spaced. The second support 6 is provided with a row of 12 magnetic rod sleeves, the 12 magnetic rod sleeves being equally spaced.

[0095] In this application, the multi-channel pipetting assembly includes a syringe driving part and a piston driving part 21. A portion of the piston driving part 21 is sleeved inside the syringe driving part, and the piston driving part 21 is vertically and vertically disposed inside the syringe driving part along a second direction.

[0096] The syringe includes a syringe barrel 23 and a piston. The syringe barrel drive unit is connected to the syringe barrel 23, thereby driving the syringe barrel 23 to move up and down in the second direction. The piston drive unit 21 is connected to the piston, and drives the piston to move up and down in the second direction.

[0097] In some embodiments, one end of the piston drive unit 21, which is fitted inside the syringe drive unit, is connected to the piston. The piston is fitted inside the syringe drive unit, and the syringe 23 is connected to the end of the syringe drive unit away from the piston drive unit 21. A portion of the piston extends into the syringe 23. Thus, the syringe drive unit and the piston drive unit 21 control the raising and lowering of the syringe 23 and the piston, respectively, to perform pipetting.

[0098] Furthermore, the drive assembly also includes a fourth drive assembly and a fifth drive assembly. The fourth drive assembly is used to drive the syringe drive unit to move up and down along the second direction, and the fifth drive assembly is used to drive the piston drive unit 21 to move up and down along the second direction.

[0099] The multi-channel pipetting assembly is located above the nucleic acid extraction and detection box. The fourth and fifth driving components drive the multi-channel pipetting assembly or the syringe driving part to move above the nucleic acid extraction and detection box, and can move downward to allow the syringe 23 to extend into the nucleic acid extraction and detection box.

[0100] The fourth drive assembly includes a fourth drive motor 11 and a fourth drive shaft. The fourth drive motor 11 is connected to the fourth drive shaft, and the fourth drive motor 11 drives the fourth drive shaft to move up and down in a second direction. The fourth drive motor 11 is connected to the fourth base plate 4, and the fourth drive shaft is connected to the fourth bracket 7. By driving the fourth drive shaft to move up and down in the second direction through the fourth drive motor 11, the fourth bracket 7 and the injection cylinder drive unit are also driven to move up and down in the second direction.

[0101] The fifth drive assembly includes a fifth drive motor 12 and a fifth drive shaft. The fifth drive motor 12 is connected to the fifth drive shaft, and the fifth drive motor 12 drives the fifth drive shaft to move up and down in a second direction. The fifth drive motor 12 is connected to the fourth bracket 7, and the fifth drive shaft is connected to the fifth bracket 9. By driving the fifth drive shaft to move up and down in the second direction through the fifth drive motor 12, the fifth bracket 9 and the piston drive unit 21 are driven to move up and down in the second direction.

[0102] The fifth bracket 9 includes a fifth fixed bracket and a fifth limiting sleeve. The fifth fixed bracket has fifth through holes at both ends, and the fifth limiting sleeve surrounds the fifth through holes. A piston drive unit 21 is connected to the fifth fixed bracket along a second direction. The fourth bracket 7 includes a fourth fixed bracket and a fourth connecting bracket. The fourth fixed bracket is connected to the fourth drive shaft and is vertically connected to the fourth base plate 4 via a fourth limiting slide rail. The fourth connecting bracket passes through the fifth limiting sleeve and the fifth through hole and is connected to the injection cylinder drive unit. When the fourth connecting bracket moves vertically, it does not affect the fifth fixed bracket. The fifth limiting sleeve can limit the offset or swaying of the fourth connecting bracket during vertical movement.

[0103] Furthermore, the fifth support 9 is provided with m columns of piston drive units 21, the spacing of the m columns of piston drive units 21 is adjustable, each column has n piston drive units 21, and the spacing of the n piston drive units 21 is adjustable; where m≥1, n≥2.

[0104] In some embodiments, the fourth support 7 is provided with m columns of syringe driving parts, the spacing between the m columns of syringe driving parts is adjustable, each column has n syringe driving parts, and the spacing between the n syringe driving parts is adjustable; where m≥1, n≥2.

[0105] In some embodiments, the fourth support 7 is provided with an injection cylinder drive assembly 22, which has m rows of mutually spaced injection cylinder drive units. The spacing between the m rows of injection cylinder drive units is adjustable, and each row has n injection cylinder drive units. The spacing between the n injection cylinder drive units is adjustable; where m≥1 and n≥2.

[0106] Furthermore, the number of extraction and purification pipetting components is at least one set, for example, the number can be 1 set, 2 sets, 3 sets, 4 sets, 5 sets, 6 sets, 7 sets, 8 sets, 9 sets, 10 sets, etc.

[0107] Furthermore, the number of magnetic rod holder assemblies 24 is at least one set, for example, the number can be 1 set, 2 sets, 3 sets, 4 sets, 5 sets, 6 sets, 7 sets, 8 sets, 9 sets, 10 sets, etc.

[0108] In some embodiments, there are two sets of the fourth support 7 and the fifth support 9, with two sets of the fourth support 7 arranged side by side and two sets of the fifth support 9 arranged side by side. There are two sets of the multi-channel pipetting assembly, and one set of the magnetic rod holder assembly 24, with two sets of multi-channel pipetting assemblies arranged side by side and the magnetic rod holder assembly 24 positioned between the two sets of multi-channel pipetting assemblies. The fourth base plate 4 has an opening channel through which both sets of multi-channel pipetting assemblies perpendicularly penetrate the fourth base plate 4.

[0109] In this application, the system further includes a third base plate 3, which is disposed above the detection box carrier assembly, and the third base plate 3 has an opening in the middle, through which the multi-channel pipetting assembly and the magnetic rod holder assembly 24 can pass when moving in the second direction.

[0110] The third base plate 3 is disposed between the fourth base plate 4 and the first base plate 1. The third base plate 3 is movably disposed on the support column 14.

[0111] A sliding sleeve is provided on the support column 14, and the third base plate 3 is sleeved on the sliding sleeve, so that the third base plate 3 slides on the support column 14 through the sliding sleeve.

[0112] Furthermore, the drive assembly also includes a sixth drive assembly, which is used to drive the third base plate 3 to move up and down along the second direction.

[0113] The sixth drive assembly includes a sixth drive motor 13 and a sixth drive shaft. The sixth drive motor 13 is connected to the sixth drive shaft, and the sixth drive motor 13 drives the sixth drive shaft to move up and down along the second direction. The sixth drive motor 13 is connected to the fourth base plate 4, and the sixth drive shaft is connected to the third base plate 3. By driving the sixth drive shaft to move up and down along the second direction, the sixth drive motor 13 drives the third base plate 3 to move up and down along the second direction.

[0114] Furthermore, a first pressure plate 17 and a second pressure plate are provided on the side surface of the third base plate 3 facing the detection box carrier assembly. The first pressure plate 17 is located above the first fixing member 15, and the second pressure plate is located above the second fixing member 16. The sixth driving assembly drives the first pressure plate 17 and the second pressure plate to descend along the second direction, so that the first pressure plate 17 and the second pressure plate press on the top of the nucleic acid extraction detection box, thereby pressing the nucleic acid extraction detection box tightly and confining it on the detection box carrier assembly, ensuring that the nucleic acid extraction detection box will not move during the nucleic acid extraction process and avoiding affecting the nucleic acid extraction process.

[0115] Furthermore, the first pressure plate 17 has a first slot on the side surface facing the nucleic acid extraction and detection box, and the second pressure plate has a second slot on the side surface facing the nucleic acid extraction and detection box. The first slot and the second slot are used to engage the nucleic acid extraction and detection box.

[0116] The number of first slots on the first pressure plate 17 is the same as the number of second slots on the second pressure plate, and each first slot corresponds to one second slot. Specifically, the number of both the first and second slots can be greater than or equal to 1. In some embodiments, the number of both the first and second slots can be greater than or equal to 5, greater than or equal to 10, or greater than or equal to 15. Specifically, the number of both the first and second slots can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, etc.

[0117] In some embodiments, the first pressure plate 17 is provided with five first card slots arranged in sequence, namely first card slot A, first card slot B, first card slot C, first card slot D, and first card slot E. The second pressure plate is provided with five second card slots arranged in sequence, namely second card slot A, second card slot B, second card slot C, second card slot D, and second card slot E. The first card slot A and the second card slot B are arranged opposite each other, the first card slot B and the second card slot B are arranged opposite each other, the first card slot C and the second card slot C are arranged opposite each other, the first card slot D and the second card slot D are arranged opposite each other, and the first card slot E and the second card slot E are arranged opposite each other.

[0118] In some embodiments, when the nucleic acid extraction detection kit is a multi-well plate, the direction from the first slot to the second slot is the extension direction of the pores of the multi-well plate, and this direction is also the first direction. The two ends of the extension direction of the pores of the multi-well plate are detachably engaged in the first slot and the second slot. That is, the two ends of the multi-well plate are respectively engaged in the first slot A and the second slot A.

[0119] In this application, the fluorescence channel assembly is disposed on the first base plate 1, and the optical fiber of the fluorescence channel assembly extends into the detection well to perform PCR detection on the sample in the nucleic acid extraction detection kit.

[0120] Furthermore, the fluorescence channel assembly includes an optical fiber sensor, with an optical fiber at one end of the sensor extending into the detection well to perform PCR detection on the sample in the nucleic acid extraction detection kit.

[0121] In this application, the temperature control component includes a heating unit 18 and a cooling unit 19. The heating unit 18 is disposed directly below the fixing sleeve 25, and the cooling unit 19 is disposed on one side of the heating unit 18, which is used to cool the heating unit 18.

[0122] In this application, the system further includes a display mechanism connected to the control mechanism for issuing instructions to the control mechanism.

[0123] The control mechanism sends commands to the detection cartridge carrier assembly, extraction and purification pipetting assembly, temperature control assembly, driving assembly, and fluorescence channel assembly via the display mechanism, and transmits the data of the detection cartridge carrier assembly, extraction and purification pipetting assembly, temperature control assembly, driving assembly, and fluorescence channel assembly to the display mechanism.

[0124] In this application, the system further includes a protection component, which comprises an ultraviolet sterilization unit, an illumination unit, and an operation warning unit. The ultraviolet sterilization unit is used to sterilize areas within the system that require sterilization, the illumination component is used to illuminate areas that require illumination, and the operation warning unit is used to monitor, warn of risks, and control the operation of various components within the system in real time, ensuring the safe and stable operation of the system and preventing accidents.

[0125] Example

[0126] The materials and test methods used in the embodiments of this application are described in a general and / or specific manner. In the following embodiments, unless otherwise specified, % means wt%, i.e., weight percentage. Reagents or instruments used, unless otherwise specified, are all commercially available conventional reagent products.

[0127] Example 1

[0128] The fully automated nucleic acid processing system of this embodiment includes a control component, a detection cartridge carrier assembly, an extraction and purification pipetting assembly, a temperature control component, a driving component, a fluorescence channel assembly, and a display component. The control mechanism sends commands to the detection cartridge carrier assembly, the extraction and purification pipetting assembly, the temperature control component, the driving component, and the fluorescence channel assembly through the display mechanism, and transmits the data of the detection cartridge carrier assembly, the extraction and purification pipetting assembly, the temperature control component, the driving component, and the fluorescence channel assembly to the display mechanism.

[0129] The detection kit carrier assembly includes a first fixing part and four sets of heating fixing parts. The first fixing part includes a first fixing member 15 and a second fixing member 16 disposed opposite to each other. Twelve detection holes and twelve sets of the heating fixing parts are arranged along a first direction between the first fixing member 15 and the second fixing member 16. Each set of heating fixing parts includes four spaced-apart fixing sleeves 25. The first fixing member 15 and the second fixing member 16 are used to restrict both ends of the nucleic acid extraction detection kit, and the fixing sleeves 25 are used to restrict a portion of the nucleic acid extraction detection kit. When the nucleic acid extraction detection kit has multiple holes, the holes requiring heating can be placed inside the fixing sleeves 25. The height of the first fixing member 15 and the second fixing member 16 is higher than the height of the fixing sleeves 25. The sidewall of the first fixing member 15 facing the second fixing member 16 has twelve sets of recessed first fixing grooves, and the sidewall of the second fixing member 16 facing the first fixing member 15 has twelve sets of recessed second fixing grooves. The twelve sets of first fixing grooves and the twelve sets of second fixing grooves are disposed opposite to each other. The two ends of the nucleic acid extraction and detection kit can be respectively snapped into the first fixing slot and the second fixing slot, thereby restricting the position of the nucleic acid extraction and detection kit.

[0130] The extraction and purification pipetting assembly includes a set of magnetic rod holder assemblies 24 and two sets of multi-channel pipetting assemblies. The two sets of multi-channel pipetting assemblies are arranged side by side, with the magnetic rod holder assemblies 24 positioned between them. The magnetic rod holder assemblies 24 are vertically and vertically positioned above the detection kit carrier assembly, and are used for agitation and / or magnetic attraction during the nucleic acid extraction process. The multi-channel pipetting assemblies are vertically and vertically positioned above the detection kit carrier assembly, and are used to drive the syringe to pipette during the nucleic acid extraction process. Each set of magnetic rod holder assemblies 24 includes 12 magnetic rods and 12 magnetic rod sleeves. The 12 magnetic rods are equally spaced, and a portion of each magnetic rod is movably disposed within the magnetic rod sleeve. Each multi-channel pipetting assembly includes a syringe drive assembly 22 and 12 piston drive units 21. The syringe drive assembly 22 includes 12 spaced-apart syringe drive units. The 12 syringe drive units are internally independent and do not communicate with each other. The external parts of the 12 syringe drive units are connected together to form a whole. A portion of the piston drive unit 21 is movably disposed within the syringe drive unit.

[0131] The system also includes a first base plate 1, a second base plate 2, a third base plate 3, a fourth base plate 4, four support columns 14, a first bracket 5, a second bracket 6, a fourth bracket 7, and a fifth bracket 9. The fourth base plate 4 is fixedly connected to the second base plate 2 via the four support columns 14. The first bracket 5, the second bracket 6, the two sets of fifth brackets 9, and the two sets of fourth brackets 7 are all connected to the fourth base plate 4. The drive assembly includes a first drive assembly, a second drive assembly, a third drive assembly, a fourth drive assembly, and a fifth drive assembly.

[0132] The first driving assembly includes a first driving motor 8, a first driving shaft, a first slider, and a first slide rail. The first driving motor 8 and the first slide rail are disposed on the second base plate 2, and the first slider is disposed on the bottom of the first base plate 1. The first driving motor 8 is connected to the first driving shaft, and the first driving shaft is connected to the slider. The first driving motor 8 can drive the first driving shaft to reciprocate along a first direction, thereby driving the first slider to reciprocate along the first direction on the first slide groove, and further driving the first base plate 1 and the various parts of the structure on the first base plate 1 to reciprocate along the first direction.

[0133] The second drive assembly includes a second drive motor 20 and a second drive shaft. The second drive motor 20 is connected to the second drive shaft, and the second drive shaft is connected to the first bracket 5. Thus, when the second drive motor 20 drives the second drive shaft to move up and down in the second direction, the first bracket 5 will also move up and down in the second direction, thereby driving the magnetic rod to move up and down in the second direction.

[0134] The third drive assembly includes a third drive motor 10 and a third drive shaft. The third drive motor 10 is connected to the third drive shaft, and the third drive shaft is connected to the second bracket 6. Thus, when the third drive motor 10 drives the third drive shaft to move up and down in the second direction, the second bracket 6 will also move up and down in the second direction, thereby driving the magnetic rod sleeve to move up and down in the second direction.

[0135] The first bracket 5 includes a first fixed bracket and a first connecting bracket. The first fixed bracket is connected to the second drive shaft, and the first connecting bracket is connected to the magnetic rod. The second bracket 6 includes a second fixed bracket and a second connecting bracket. The second fixed bracket is connected to the third drive shaft, and the second connecting bracket is connected to the magnetic rod sleeve. The first connecting bracket has a first through hole, and the second connecting bracket passes through the first through hole, causing the first connecting bracket to be positioned above the magnetic rod sleeve. A first limiting cylinder is provided on the first connecting bracket, surrounding the first through hole. The second connecting bracket passes through the first limiting cylinder and the first through hole. When the second connecting bracket moves up and down, the first connecting bracket is not affected. The first limiting cylinder can limit the offset or sway of the second connecting bracket during up and down movement.

[0136] The fourth drive assembly includes a fourth drive motor 11 and a fourth drive shaft. The fourth drive motor 11 is connected to the fourth drive shaft, and the fourth drive motor 11 drives the fourth drive shaft to move up and down along a second direction. The fourth drive motor 11 is connected to the fourth base plate 4, and the fourth drive shaft is connected to the fourth bracket 7. By driving the fourth drive shaft to move up and down along the second direction via the fourth drive motor 11, the fourth bracket 7, the syringe drive unit, and the syringe itself are moved up and down along the second direction.

[0137] The fifth drive assembly includes a fifth drive motor 12 and a fifth drive shaft. The fifth drive motor 12 is connected to the fifth drive shaft, and the fifth drive motor 12 drives the fifth drive shaft to move up and down along a second direction. The fifth drive motor 12 is connected to the fourth bracket 7, and the fifth drive shaft is connected to the fifth bracket 9. By driving the fifth drive shaft to move up and down along the second direction via the fifth drive motor 12, the fifth bracket 9, the piston drive unit 21, and the piston are driven to move up and down along the second direction.

[0138] The fifth bracket 9 includes a fifth fixed bracket and a fifth limiting sleeve. The fifth fixed bracket has fifth through holes at both ends, and the fifth limiting sleeve surrounds the fifth through holes. Twelve pistons are connected to the fifth fixed bracket along the second direction. The fourth bracket 7 includes a fourth fixed bracket and a fourth connecting bracket. The fourth fixed bracket is connected to the fourth drive shaft and is vertically connected to the fourth base plate 4 via a fourth limiting slide rail. The fourth connecting bracket passes through the fifth limiting sleeve and the fifth through holes and connects to the injection cylinder drive assembly 22. When the fourth connecting bracket moves vertically, it does not affect the fifth fixed bracket. The fifth limiting sleeve limits the offset or swaying of the fourth connecting bracket during vertical movement. Two sets of fourth brackets 7 and two sets of fifth brackets 9 are arranged side-by-side.

[0139] The sixth drive assembly includes a sixth drive motor 13 and a sixth drive shaft. The sixth drive motor 13 is connected to the sixth drive shaft, and the sixth drive motor 13 drives the sixth drive shaft to move up and down along the second direction. The sixth drive motor 13 is fixedly connected to the fourth base plate 4, and the sixth drive shaft is connected to the third base plate 3. By driving the sixth drive shaft to move up and down along the second direction through the sixth drive motor 13, the third base plate 3 is also driven to move up and down along the second direction.

[0140] The third base plate 3 is disposed between the fourth base plate 4 and the first base plate 1. A sliding sleeve is provided on the support column 14, and the third base plate 3 is sleeved on the sliding sleeve, so that the third base plate 3 can slide on the support column 14 through the sliding sleeve. The third base plate 3 is located above the detection box carrier assembly, and the third base plate 3 has an opening in the middle. When the multi-channel pipetting assembly and the magnetic rod holder assembly 24 move in the second direction, they can extend into the opening and then into the nucleic acid extraction detection box. The third base plate 3 has a first pressure plate 17 and a second pressure plate on the side surface facing the detection box carrier assembly. The first pressure plate 17 is located above the first fixing member 15, and the second pressure plate is located above the second fixing member 16. The first pressure plate 17 has 12 first slots on the side surface facing the nucleic acid extraction detection box, and the second pressure plate has 12 second slots on the side surface facing the nucleic acid extraction detection box. The first slots and the second slots are used to engage the nucleic acid extraction detection box.

[0141] The fluorescence channel assembly is disposed on the first base plate 1. The fluorescence channel assembly includes an optical fiber sensor. The optical fiber of the optical fiber sensor extends into the detection well to perform PCR detection on the sample in the nucleic acid extraction detection kit.

[0142] The temperature control component includes a heating unit 18 and a cooling unit 19. The heating unit 18 is located directly below the fixing sleeve 25, and the cooling unit 19 is located on one side of the heating unit 18, which is used to cool the heating unit 18.

[0143] In this embodiment, the system is used by placing the nucleic acid extraction detection box within the detection box carrier assembly and securing it with the first fixing member 15 and the second fixing member 16. Then, the sixth driving component lowers the third base plate 3, causing the first pressure plate 17 and the second pressure plate to press against the top of the nucleic acid extraction detection box. A plasma sample is then injected into the nucleic acid extraction detection box. The second, third, fourth, and fifth driving components adjust the raising and lowering of the magnetic rod holder assembly 24 and the multi-channel pipetting assembly to perform nucleic acid extraction and purification. After nucleic acid extraction and purification, the optical fiber of the fluorescence channel assembly performs PCR detection through the detection well. The fully automated nucleic acid methylation processing system described in this application completes the entire process from plasma sample entry to obtaining the PCR detection result of the target DNA entirely by the equipment, requiring no manual intervention, achieving a high degree of automation in nucleic acid methylation extraction and detection. Furthermore, the various mechanisms in this application are highly independent, allowing for collaborative work between mechanisms while individual mechanisms can also perform their respective tasks. The relative positions of all mechanisms can be varied depending on space constraints, offering flexibility in their relative positions.

[0144] [Amended according to Regulation 26, 30.12.2025] Although the embodiments of this application have been described above in conjunction with the accompanying drawings, this application is not limited to the specific embodiments and application fields described above. The specific embodiments described above are merely illustrative and instructive, not restrictive. Those skilled in the art can make many other forms based on the guidance of this specification and without departing from the scope of protection of the claims of this application, and these are all within the scope of protection of this application.

Claims

1. A fully automated nucleic acid processing system, wherein, It includes control components, detection cartridge carrier components, extraction and purification pipetting components, temperature control components, drive components, and fluorescence channel components, among which... The control components control the detection kit carrier assembly, the extraction and purification pipetting assembly, the temperature control assembly, the drive assembly, and the fluorescence channel assembly, respectively. The detection kit carrier assembly is used to hold the nucleic acid extraction detection kit; The detection box carrier assembly includes a first fixing part and at least one set of heating fixing parts. The first fixing part includes a first fixing member and a second fixing member disposed opposite to each other. At least one set of heating fixing parts is disposed between the first fixing member and the second fixing member. Each set of heating fixing parts includes at least two spaced fixing sleeves. The first fixing member and the second fixing member are respectively used to restrict the two ends of the nucleic acid extraction detection box. The fixing sleeves are used to restrict a part of the nucleic acid extraction detection box. The extraction and purification pipetting assembly is vertically and vertically positioned above the detection kit carrier assembly, and is used for pipetting, shaking, and / or magnetic suction during nucleic acid extraction. The driving component is used to drive the detection box carrier assembly to reciprocate along the first direction and drive the extraction and purification pipetting assembly to move up and down along the second direction. The temperature control component is located below the fixing sleeve and is used to provide temperature conditions for the nucleic acid extraction and detection box inside the fixing sleeve; The fluorescence channel assembly is used for PCR detection of samples in the nucleic acid extraction and detection kit.

2. The system according to claim 1, wherein, The sidewall of the first fastener facing the second fastener has at least one set of first fastening grooves recessed inward therein. The second fastener has at least one set of second fastening grooves recessed into its sidewall facing the first fastener.

3. The system according to claim 2, wherein, A detection hole is also provided between the first fixing member and the second fixing member. The detection hole is located on the side closer to the first fixing member. The number of heating fixing parts and the number of detection holes are equal to the number of the first fixing groove and the second fixing groove.

4. The system according to claim 3, wherein, The system also includes a first base plate, the driving component includes a first driving component, the temperature control component is disposed on the first base plate, the first driving component is used to drive the first base plate to reciprocate along a first direction, thereby driving the temperature control component and the detection box carrier assembly to reciprocate along the first direction.

5. The system according to claim 4, wherein, The system also includes a second base plate, on which the first drive component is disposed, and the first base plate moves along a first direction on the second base plate.

6. The system according to claim 1, wherein, The extraction and purification pipetting assembly includes a magnetic rod holder assembly and a multi-channel pipetting assembly. The magnetic rod holder assembly is vertically and vertically positioned above the detection kit carrier assembly and is used for oscillation and / or magnetic attraction during the nucleic acid extraction process. The multi-channel pipetting assembly is vertically and vertically positioned above the detection kit carrier assembly and is used to drive the syringe to pipette during the nucleic acid extraction process.

7. The system according to claim 6, wherein, The system also includes a fourth base plate, a first bracket, a second bracket, a fourth bracket, and a fifth bracket. The fourth base plate is fixedly connected to the second base plate via support columns. The first bracket, the second bracket, the fourth bracket, and the fifth bracket are all connected to the fourth base plate.

8. The system according to claim 7, wherein, The magnetic rod holder assembly includes multiple magnetic rods and magnetic rod sleeves arranged in an array. The magnetic rods are located above the magnetic rod sleeves and can move downwards to extend into the magnetic rod sleeves.

9. The system according to claim 8, wherein, The driving assembly further includes a second driving assembly and a third driving assembly. The second driving assembly is used to drive the magnetic rod to move up and down along the second direction, and the third driving assembly is used to drive the magnetic rod sleeve to move up and down along the second direction. The magnetic rod holder assembly is located above the nucleic acid extraction and detection box. The second and third driving components drive the magnetic rod holder assembly or magnetic rod sleeve to move above the nucleic acid extraction and detection box, and can move downward to extend the magnetic rod holder assembly or magnetic rod sleeve into the nucleic acid extraction and detection box.

10. The system according to claim 8 or 9, wherein, The first support is provided with m columns of magnetic bars, the spacing of the m columns of magnetic bars is adjustable, each column has n magnetic bars, and the spacing of the n magnetic bars is adjustable; The second support is provided with m columns of magnetic rod sleeves, the spacing of the m columns of magnetic rod sleeves is adjustable, each column has n magnetic rod sleeves, and the spacing of the n magnetic rod sleeves is adjustable; Where m≥1, n≥2.

11. The system according to claim 7, wherein, The multi-channel pipetting assembly includes a syringe drive and a piston drive. A portion of the piston drive is fitted inside the syringe drive, and the piston drive is vertically and vertically disposed inside the syringe drive along a second direction. The syringe drive unit is used to drive the syringe syringe to move up and down in the second direction; The piston drive unit is used to drive the piston of the syringe to move up and down in the second direction.

12. The system according to claim 11, wherein, The drive assembly further includes a fourth drive assembly and a fifth drive assembly. The fourth drive assembly is used to drive the syringe drive unit to move up and down along the second direction, and the fifth drive assembly is used to drive the piston drive unit to move up and down along the second direction. The multi-channel pipetting assembly is located above the nucleic acid extraction and detection box. The fourth and fifth driving components drive the multi-channel pipetting assembly or the syringe driving part to move above the nucleic acid extraction and detection box, and can move downward to allow the syringe to extend into the nucleic acid extraction and detection box.

13. The system according to claim 11 or 12, wherein, The fourth support is provided with m columns of syringe drive units, the spacing between the m columns of syringe drive units is adjustable, each column has n syringe drive units, and the spacing between the n syringe drive units is adjustable, or The fourth support is provided with an injection cylinder driving assembly, which has m columns of mutually spaced injection cylinder driving parts. The spacing between the m columns of injection cylinder driving parts is adjustable. Each column has n injection cylinder driving parts, and the spacing between the n injection cylinder driving parts is adjustable. The fifth support is provided with m columns of piston drive units, the spacing between the m columns of piston drive units is adjustable, each column has n piston drive units, and the spacing between the n piston drive units is adjustable; where m≥1, n≥2.

14. The system according to claim 1, wherein, The system also includes a third base plate, which is disposed above the detection box carrier assembly and has an opening in the middle. When the multi-channel pipetting assembly and the magnetic rod holder assembly move along the second direction, they pass through the opening.

15. The system according to claim 14, wherein, The drive assembly also includes a sixth drive assembly, which is used to drive the third base plate to move up and down along the second direction.

16. The system according to claim 15, wherein, The third base plate has a first pressure plate and a second pressure plate on one side of the surface facing the test box carrier assembly. The first pressure plate is located above the first fixing member, and the second pressure plate is located above the second fixing member. The sixth driving assembly drives the first pressure plate and the second pressure plate to descend in the second direction, so that the first pressure plate and the second pressure plate press on the top of the nucleic acid extraction test box, thereby fixing the nucleic acid extraction test box on the test box carrier assembly.

17. The system according to claim 16, wherein, The first pressure plate has a first slot on the side of its surface facing the nucleic acid extraction and detection box, and the second pressure plate has a second slot on the side of its surface facing the nucleic acid extraction and detection box. The first slot and the second slot are used to engage the nucleic acid extraction and detection box.

18. The system according to claim 4, wherein, The fluorescence channel assembly is disposed on the first base plate, and the optical fiber of the fluorescence channel assembly extends into the detection well to perform PCR detection on the sample in the nucleic acid extraction detection kit.

19. The system according to claim 1, wherein, The temperature control component includes a heating unit and a cooling unit. The heating unit is located directly below the fixing sleeve, and the cooling unit is located on one side of the heating unit and is used to cool the heating unit.

20. The system according to claim 1, wherein, The system also includes a display mechanism connected to the control mechanism for issuing commands to the control mechanism.

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