Quantitative sample loading device for blood samples and sample loading and cleaning method

WO2026199625A1PCT designated stage Publication Date: 2026-10-01SHANDONG ACCURDX BIO-TECHNOLOGY CO LTD
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Patent Information

Application Number
PCT/CN2025/087541
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2025-03-28
Filing Date
2025-04-07
Publication Date
2026-10-01

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Abstract

A quantitative sample loading device for blood samples and a sample loading and cleaning method, comprising a bracket (1), and a first plunger pump (3), a second plunger pump (4), a first blood sensor (5), a second blood sensor (6), a first tube clamp valve (7), a second tube clamp valve (8), a third tube clamp valve (9), a fourth tube clamp valve (10), and a confluence pool (11) mounted on the bracket (1), wherein the individual components are connected to one another by means of delivery tubes. By providing the first blood sensor (5) and the second blood sensor (6), the sampling volume of an aspirated blood sample is precisely controlled; by means of the cooperation of two plunger pumps and tube clamp valves, residual blood samples and pure water remaining in the tubes can be eliminated, thereby preventing the residual blood samples and pure water in the tubes from increasing the detection error.
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Description

A blood sample quantitative loading device and a loading and cleaning method Technical Field

[0001] This invention relates to the field of medical device technology, specifically to a blood sample quantitative addition device and a method for adding and cleaning the sample. Background Technology

[0002] The amount of blood sample taken in in vitro diagnostic instruments is crucial to the entire testing process. A serious deviation in the sample amount will lead to inaccurate results and waste of blood. If there is not much blood left in the test tube, too much blood left in the tube during the aspiration process will make it impossible to perform the test. There may be a small amount of cleaning fluid remaining in the aspiration tube, which will flow into the front part of the blood sample during aspiration, causing errors in the test results. Summary of the Invention

[0003] The purpose of this invention is to solve the problems mentioned in the background art, and therefore proposes a blood sample quantitative addition device and a method for adding and cleaning the sample. The device disclosed in this invention, by setting a first blood sensor and a second blood sensor, precisely controls the amount of blood sample taken, reducing detection errors and improving detection accuracy; through the cooperation of two sets of plunger pump clamp valves, it can remove residual blood sample and pure water from the tube, avoiding the increase in detection errors caused by residual blood sample and pure water, further improving detection accuracy.

[0004] The technical solution adopted by this invention to solve its technical problem is:

[0005] A blood sample quantitative dispensing device includes a support, a first plunger pump and a clamp valve assembly mounted on the support, which are connected by a delivery pipe assembly. The device also includes a second plunger pump, a first blood sensor, a second blood sensor, and a manifold mounted on the support. The first plunger pump is located on the lower left side of the front of the support, and the second plunger pump is located on the lower right side of the front of the support. The first and second plunger pumps provide power for the device to operate, completing the aspiration and mixing of sample blood and diluent, and simultaneously cleaning the device. The first blood sensor is located on the top surface of the support, and the second blood sensor is located on the right side of the support. The first and second blood sensors detect the flow positions of the sample blood and mixed detection solution, thereby precisely controlling the sample aspiration volume. The manifold is located on the front of the support, above the first plunger pump. The first plunger pump, the second plunger pump, the first blood sensor, the second blood sensor, the clamp valve assembly, and the manifold are connected by a delivery pipe assembly, with a valve connector assembly at the connection point.

[0006] Preferably, the clamp valve assembly includes a first clamp valve, a second clamp valve, a third clamp valve, and a fourth clamp valve. The first clamp valve is located on the left side of the support, while the second, third, and fourth clamp valves are located on the front of the support, above the second plunger pump. A first delivery tube passes through the first blood sensor. One end of the first delivery tube is connected to a sample needle, through which sample blood is drawn. The other end is connected to the first clamp valve, and then connected to the manifold via a second valve connector. Above the manifold, the first delivery tube is connected to the third delivery tube via the first valve connector. The other end of the third delivery tube is connected to the fourth delivery tube via a ninth valve connector. The other end of the fourth delivery tube passes through the second blood sensor and connects to the second delivery tube. The other end of the second delivery tube is connected to the fifth valve connector. The head is connected to the first plunger pump. The fifth delivery tube is fixed to the right side of the manifold via the third pipe valve connector. The fifth delivery tube passes through the second clamp valve and is then connected to the second plunger pump via the seventh pipe valve connector. The seventh delivery tube is fixed to the front of the manifold via the fourth pipe valve connector. The seventh delivery tube passes through the third clamp valve and is then connected to the sample loading seat. The prepared sample is delivered to the erythrocyte sedimentation rate (ESR) measuring tube through the sample loading seat. The eighth delivery tube is fixed to the side of the first plunger pump via the sixth pipe valve connector. The eighth delivery tube passes through the fourth clamp valve and is then connected to the waste discharge pipe. After the device is cleaned, the cleaning waste liquid is discharged through the waste discharge pipe. The sixth delivery tube is fixed to the second plunger pump via the eighth pipe valve connector. The sixth delivery tube passes through the second clamp valve and is then connected to the diluent storage tank. The diluent is delivered to the manifold via the sixth delivery tube.

[0007] Preferably, a pressure sensor is also provided on the top surface of the support. The pressure sensor is connected to the fourth delivery pipe through the ninth delivery pipe, and a tenth pipe valve connector is provided at the connection position. The pressure sensor can detect the pressure of the internal pipes of the device in real time to avoid air leakage from the device affecting the sample absorption volume.

[0008] Preferably, an L-shaped bracket fixing plate is provided at the bottom of the bracket to improve the fixing reliability of the bracket.

[0009] Preferably, the device also includes a rotating fixed stage, which has two sets of rotating components. The two sets of rotating components fix the sample application seat and the extraction seat respectively. A tenth delivery tube is provided above the extraction seat. The other end of the tenth delivery tube is connected to a diaphragm pump. The successfully prepared sample is delivered into the erythrocyte sedimentation rate (ESR) measuring tube through the cooperation of the sample application seat and the extraction seat. The diaphragm pump provides power to draw pure water for cleaning the internal pipes of the device.

[0010] Preferably, the inner diameter of the third delivery tube is larger than that of the first and fourth delivery tubes. The blood sample mixed with the diluent flows through the third delivery tube, where it undergoes a change in diameter and mixing again to ensure thorough mixing of the blood and the diluent.

[0011] Preferably, the first, third, and fourth clamp valves are single-pipe clamp valves, and the second clamp valve is a double-pipe clamp valve.

[0012] Preferably, the device also includes a control device, which is electrically connected to the pressure sensor, the first plunger pump, the second plunger pump, the first blood sensor, and the second blood sensor, making the device more intelligent and its operation smoother.

[0013] A method for adding blood samples using a quantitative blood sample addition device includes the following steps:

[0014] S1. Close the third and fourth clamp valves, and open the first and second clamp valves;

[0015] S2. The sample arm needle passes through the test tube cap and enters the test tube. The first plunger pump runs and draws up the sample blood. After the sample blood flows through the first blood sensor, the sample arm needle lifts up, and the first plunger pump continues to work. The sample blood flows through the first clamp valve and reaches the manifold. At the same time, the second plunger pump starts to work, and the sixth delivery tube extends into the diluent storage to draw up the diluent. The diluent flows through the second clamp valve to the second plunger pump, and then through the fifth delivery tube and the second clamp valve to be delivered to the manifold, where it is initially mixed.

[0016] S3. The initially mixed sample moves upward under the action of the first and second plunger pumps, passes through the third and fourth delivery tubes, and reaches the second blood sensor. At this time, the first and second plunger pumps stop working.

[0017] S4. The first clamp valve is closed, the third clamp valve is opened, the first plunger pump starts to pump back, the prepared sample flows back through the manifold and the third clamp valve, through the seventh delivery tube and through the sample holder, and is injected into the erythrocyte sedimentation rate measuring tube, thus completing a quantitative sample loading process.

[0018] A method for cleaning a blood sample quantitative dispensing device includes the following steps:

[0019] S1. The rotating component inside the rotating station drives the sample dispensing seat and the extraction seat to rotate, reaching the cleaning position, as shown in the figure;

[0020] S2. The diaphragm pump starts to first extract the residual liquid in the device pipeline;

[0021] S3. After the residue extraction is completed, the sample arm needle is placed into pure water. The diaphragm pump continues to work to extract pure water. The pure water passes through the first delivery pipe, the first clamp valve and the manifold. At the same time, the first plunger pump starts to work, and the pure water will flow upward through the third delivery pipe and the fourth delivery pipe.

[0022] S4. The diaphragm pump continues to work. Pure water passes through the manifold, the seventh delivery pipe and the third clamp valve, and is discharged into the sample dosing seat and the extraction seat. Finally, it is discharged through the diaphragm pump.

[0023] S5. The diaphragm pump continuously draws pure water, while the first plunger pump works synchronously to repeatedly clean the third and fourth delivery pipes three times to complete the cleaning process.

[0024] Compared with the prior art, the beneficial effects of the present invention are:

[0025] 1. The device disclosed in this invention, by setting a first blood sensor and a second blood sensor, precisely controls the sampling amount of blood sample, reduces detection error, and improves detection accuracy; by cooperating with two sets of plunger pump clamp valves, it can remove residual blood sample and pure water in the tube, avoid the residual blood sample and pure water in the tube from increasing the detection error, and further improve the detection accuracy.

[0026] 2. The device disclosed in this invention uses a pressure sensor to detect the pressure of the internal pipes in real time, thereby preventing air leakage from affecting the sample intake, further reducing device error and improving detection accuracy.

[0027] 3. The device disclosed in this invention provides power for cleaning by setting up a rotating fixed platform, an extraction seat and a diaphragm pump, thereby accelerating the cleaning rate, improving work efficiency, improving cleaning effect and thus improving detection accuracy.

[0028] 4. The third delivery tube with a large background is provided in the device disclosed in this invention. When the sample passes through, the channel diameter changes and the blood and diluent are mixed evenly, so that the blood and diluent are mixed more thoroughly, thereby improving the detection accuracy. Attached Figure Description

[0029] Figure 1 is a schematic diagram of the front structure of the device disclosed in this invention;

[0030] Figure 2 is a schematic diagram of the rear structure of the device disclosed in this invention;

[0031] Figure 3 is a front isometric view of the device disclosed in this invention.

[0032] Figure 4 is a schematic diagram of the rotating fixed platform and auxiliary equipment of the device disclosed in this invention.

[0033] The components include: 1. Bracket; 101. Bracket fixing plate; 2. Pressure sensor; 3. First plunger pump; 4. Second plunger pump; 5. First blood sensor; 6. Second blood sensor; 7. First clamp valve; 8. Second clamp valve; 9. Third clamp valve; 10. Fourth clamp valve; 11. Manifold; 12. Valve connector assembly; 121. First valve connector; 122. Second valve connector; 123. Third valve connector; 124. Fourth valve connector; 125. Fifth valve connector; 126. Sixth valve connector. 127. Seventh valve connector; 128. Eighth valve connector; 129. Ninth valve connector; 1210. Tenth valve connector; 13. Conveying pipe assembly; 131. First conveying pipe; 132. Second conveying pipe; 133. Third conveying pipe; 134. Fourth conveying pipe; 135. Fifth conveying pipe; 136. Sixth conveying pipe; 137. Seventh conveying pipe; 138. Eighth conveying pipe; 139. Ninth conveying pipe; 1310. Tenth conveying pipe; 14. Rotating fixed table; 15. Sample feeding seat; 16. Extraction seat. Embodiments of the present invention

[0034] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0035] Example 1, as shown in Figures 1-4, a blood sample quantitative dispensing device includes a support 1, a first plunger pump 3 and a clamp valve assembly mounted on the support 1, which are connected by a delivery tube assembly 13. It also includes a second plunger pump 4, a first blood sensor 5, a second blood sensor 6, and a manifold 11 mounted on the support 1. The first plunger pump 3 is located on the lower left of the front of the support 1, and the second plunger pump 4 is located on the lower right of the front of the support 1. The first plunger pump 3 and the second plunger pump 4 provide power for the device to operate and complete the aspiration of sample blood and diluent. The device operates in a mixed manner, simultaneously cleaning the equipment. The first blood sensor 5 is located on the top surface of the support 1, and the second blood sensor 6 is located on the right side of the support 1. The first blood sensor 5 and the second blood sensor 6 detect the flow position of the sample blood and the mixed test solution, thereby accurately controlling the sample aspiration volume. The manifold 11 is located on the front of the support 1, above the first plunger pump 3. The first plunger pump 3, the second plunger pump 4, the first blood sensor 5, the second blood sensor 6, the clamp valve assembly, and the manifold 11 are connected by the delivery pipe assembly 13, and the connection point is equipped with a pipe valve connector assembly 12.

[0036] The clamp valve assembly includes a first clamp valve 7, a second clamp valve 8, a third clamp valve 9, and a fourth clamp valve 10. The first clamp valve 7 is located on the left side of the support 1, while the second clamp valve 8, the third clamp valve 9, and the fourth clamp valve 10 are located on the front of the support 1, above the second plunger pump 4. The first blood sensor 5 passes through a first delivery tube 131. One end of the first delivery tube 131 is connected to a sample needle, through which sample blood is drawn. The other end is connected to the first clamp valve 7, and then connected to the manifold 11 via a second valve connector 122. Above the manifold 11, the first valve connector 121 connects to the third delivery tube 133. The other end of the third delivery tube 133 is connected to the fourth delivery tube 134 via a ninth valve connector 129. The other end of the fourth delivery tube 134 passes through the second blood sensor 6 and connects to the second delivery tube 132. The other end of the second delivery tube 132 is connected to the first plunger via a fifth valve connector 125. Pump 3 is connected. The fifth delivery tube 135 is fixed to the right side of the manifold 11 via the third pipe valve connector 123. The fifth delivery tube 135 passes through the second clamp valve 8 and is connected to the second plunger pump 4 via the seventh pipe valve connector 127. The seventh delivery tube 137 is fixed to the front of the manifold 11 via the fourth pipe valve connector 124. The seventh delivery tube 137 passes through the third clamp valve 9 and is connected to the sample loading seat 15. The prepared sample is delivered to the erythrocyte sedimentation rate (ESR) measuring tube through the sample loading seat 15. The eighth delivery tube 138 is fixed to the side of the first plunger pump 3 via the sixth pipe valve connector 126. The eighth delivery tube 138 passes through the fourth clamp valve 10 and is connected to the waste discharge tube. After the device is cleaned, the cleaning waste liquid is discharged through the waste discharge tube. The sixth delivery tube 136 is fixed to the second plunger pump 4 via the eighth pipe valve connector 128. The sixth delivery tube 136 passes through the second clamp valve 8 and is connected to the diluent storage tank. The diluent is delivered to the manifold 11 through the sixth delivery tube 136.

[0037] As shown in Figure 4, the device disclosed in this invention also includes a rotating fixed stage 14. Two sets of rotating components are arranged inside the rotating fixed stage 14. The two sets of rotating components fix the sample application seat 15 and the extraction seat 16 respectively. A tenth delivery tube 1310 is arranged above the extraction seat 16. The other end of the tenth delivery tube 1310 is connected to a diaphragm pump. The successfully prepared sample is delivered into the erythrocyte sedimentation rate measuring tube through the cooperation of the sample application seat 15 and the extraction seat 16. The diaphragm pump provides power to draw pure water for cleaning the internal pipes of the device.

[0038] As shown in Figures 1 and 2, the first clamp valve 7, the third clamp valve 9 and the fourth clamp valve 10 are single-pipe clamp valves, and the second clamp valve 8 is a double-pipe clamp valve.

[0039] In Example 2, based on the above examples, a pressure sensor 2 is also provided on the top surface of the support 1. The pressure sensor 2 is connected to the fourth delivery pipe 134 through the ninth delivery pipe 139, and a tenth pipe valve connector 1210 is provided at the connection position. The pressure sensor 2 detects the pressure of the internal pipes of the device in real time to prevent air leakage from affecting the sample absorption volume. An L-shaped support fixing plate 101 is provided at the bottom of the support 1 to improve the fixation reliability of the support 1.

[0040] In Example 3, based on the above examples, the inner diameter of the third delivery tube 133 is larger than the inner diameters of the first delivery tube 131 and the fourth delivery tube 134. The blood sample mixed with the diluent flows through the third delivery tube 133, and the diameter is changed again in the third delivery tube 133 to mix the blood and the diluent thoroughly, making the mixture of the diluent and the blood more complete.

[0041] Example 4, based on the above examples, also includes a control device. The control device is electrically connected to the pressure sensor 2, the first plunger pump 3, the second plunger pump 4, the first blood sensor 5, and the second blood sensor 6, making the device more intelligent and its operation smoother.

[0042] Example 5: A sample addition method using a blood sample quantitative addition device disclosed in this invention, the specific steps of which are as follows:

[0043] S1. Close the third clamp valve 9 and the fourth clamp valve 10, and open the first clamp valve 7 and the second clamp valve 8;

[0044] S2. The sample arm needle passes through the test tube cap and enters the test tube. The first plunger pump 3 runs and draws up the sample blood. After the sample blood flows through the first blood sensor 5, the sample arm needle lifts up, and the first plunger pump 3 continues to work. The sample blood flows through the first clamp valve 7 and reaches the manifold 11. At the same time, the second plunger pump 4 starts to work, and the sixth delivery tube 136 extends into the diluent storage to draw up the diluent. The diluent flows through the second clamp valve 8 to the second plunger pump 4, and then through the fifth delivery tube 135 and the second clamp valve 8 to be delivered to the manifold 11, where it is initially mixed.

[0045] S3. The initially mixed sample moves upward under the action of the first plunger pump 3 and the second plunger pump 4, and reaches the second blood sensor 6 through the third delivery tube 133 and the fourth delivery tube 134. At this time, the first plunger pump 3 and the second plunger pump 4 stop working.

[0046] In the above steps, the initially mixed sample is mixed again in the third delivery tube 133 by changing the diameter to ensure that the blood and diluent are fully mixed, and the mixed sample completes the second mixing.

[0047] S4. The first clamp valve 7 is closed, the third clamp valve 9 is opened, the first plunger pump 3 starts to pump back, the prepared sample flows back, through the manifold 11 and the third clamp valve 9, through the seventh delivery tube 137 and through the sample loading seat 15, and is injected into the erythrocyte sedimentation rate measuring tube through the sample loading seat 15, thus completing a quantitative sample loading process.

[0048] Example 6: A cleaning method for a blood sample quantitative dispensing device disclosed in this invention, the specific steps of which are as follows:

[0049] S1. The rotating component inside the rotating fixed stage 14 drives the sample feeding seat 15 and the extraction seat 16 to rotate, reaching the cleaning position, as shown in Figure 4;

[0050] S2. The diaphragm pump starts to first extract the residual liquid in the device pipeline;

[0051] S3. After the residue extraction is completed, the sample arm needle is placed into pure water, and the diaphragm pump continues to work to extract pure water. The pure water passes through the first delivery pipe 131, the first clamp valve 7 and the manifold 11. At the same time, the first plunger pump 3 starts to work, and the pure water will flow upward through the third delivery pipe 133 and the fourth delivery pipe 134.

[0052] S4. The diaphragm pump continues to work, and pure water passes through the manifold 11, the seventh delivery pipe 137 and the third clamp valve 9, and is discharged into the sample dosing seat 15 and the extraction seat 16, and is finally discharged through the diaphragm pump.

[0053] S5. The diaphragm pump continues to work to draw pure water, and the first plunger pump 3 works synchronously to repeatedly clean the third delivery pipe 133 and the fourth delivery pipe 134 three times to complete the cleaning work.

[0054] In the description of this invention, the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "vertical," and "horizontal," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only to describe the invention and not to require the invention to be constructed or operated in a specific orientation; therefore, they should not be construed as limitations on the invention. The terms "connected" and "linked" in this invention should be interpreted broadly. For example, they can refer to a connection or a detachable connection; they can refer to a direct connection or an indirect connection through intermediate components. Those skilled in the art can understand the specific meaning of the above terms based on the specific circumstances.

[0055] The above description represents the preferred mode of operation of the present invention. The specific operational modes are provided solely for a better understanding of the invention's concept. Those skilled in the art can make various improvements or equivalent substitutions based on the principles of this invention, and these improvements or equivalent substitutions are also considered to fall within the scope of protection of this invention.

Claims

1. A blood sample quantitative dispensing device, comprising a support (1), a first plunger pump (3) and a clamp valve assembly mounted on the support (1), wherein the first plunger pump (3) and the clamp valve assembly are connected via a delivery tube assembly (13), characterized in that: It also includes a second plunger pump (4), a first blood sensor (5), a second blood sensor (6) and a manifold (11) installed on the bracket (1). The first plunger pump (3) is located on the lower left of the front of the bracket (1), the second plunger pump (4) is located on the lower right of the front of the bracket (1), the first blood sensor (5) is located on the top surface of the bracket (1), the second blood sensor (6) is located on the right side of the bracket (1), and the manifold (11) is located on the front of the bracket (1) above the first plunger pump (3). The first plunger pump (3), the second plunger pump (4), the first blood sensor (5), the second blood sensor (6), the clamp valve assembly and the manifold (11) are connected through a delivery pipe assembly (13), and a pipe valve connector assembly (12) is provided at the connection point.

2. The blood sample quantitative dispensing device according to claim 1, characterized in that: The clamp valve assembly includes a first clamp valve (7), a second clamp valve (8), a third clamp valve (9), and a fourth clamp valve (10). The first clamp valve (7) is located on the left side of the support (1), and the second clamp valve (8), the third clamp valve (9), and the fourth clamp valve (10) are located on the front of the support (1), above the second plunger pump (4). The first blood sensor (5) passes through the first delivery tube (131). One end of the first delivery tube (131) is connected to the sample arm needle, and the other end is connected to the first clamp valve (7). Then, it is connected to the manifold (11) through the second valve connector (122). The manifold (11) is connected to the third delivery tube (133) above the first valve connector (121). The other end of the third delivery tube (133) is connected to the fourth delivery tube (134) through the ninth valve connector (129). The other end of the fourth delivery tube (134) passes through the second blood sensor (6) and is connected to the second delivery tube (132). The other end of the second delivery pipe (132) is connected to the first plunger pump (3) via the fifth pipe valve connector (125). The right side of the manifold (11) is fixed to the fifth delivery pipe (135) via the third pipe valve connector (123). The fifth delivery pipe (135) passes through the second clamp valve (8) and is then connected to the second plunger pump (4) via the seventh pipe valve connector (127). The front of the manifold (11) is fixed to the seventh delivery pipe (137) via the fourth pipe valve connector (124). The tube (137) passes through the third clamp valve (9) and is connected to the sample holder (15). The first plunger pump (3) fixes the eighth delivery tube (138) on the side through the sixth pipe valve connector (126). The eighth delivery tube (138) passes through the fourth clamp valve (10) and is connected to the waste discharge tube. The second plunger pump (4) fixes the sixth delivery tube (136) through the eighth pipe valve connector (128). The sixth delivery tube (136) passes through the second clamp valve (8) and is connected to the diluent storage.

3. The blood sample quantitative dispensing device according to claim 2, characterized in that: The top surface of the bracket (1) is also provided with a pressure sensor (2), which is connected to the fourth delivery pipe (134) through the ninth delivery pipe (139), and the connection position is provided with a tenth pipe valve connector (1210).

4. The blood sample quantitative dispensing device according to claim 2, characterized in that: The bracket (1) has an L-shaped bracket fixing plate (101) at its bottom.

5. The blood sample quantitative dispensing device according to claim 2, characterized in that: It also includes a rotating fixed stage (14), in which two sets of rotating components are provided. The two sets of rotating components fix the sample feeding seat (15) and the extraction seat (16) respectively. A tenth delivery pipe (1310) is provided above the extraction seat (16), and the other end of the tenth delivery pipe (1310) is connected to the diaphragm pump.

6. The blood sample quantitative dispensing device according to claim 2, characterized in that: The inner diameter of the third conveying pipe (133) is larger than the inner diameters of the first conveying pipe (131) and the fourth conveying pipe (134).

7. The blood sample quantitative dispensing device according to claim 2, characterized in that: The first clamp valve (7), the third clamp valve (9) and the fourth clamp valve (10) are single-pipe clamp valves, and the second clamp valve (8) is a double-pipe clamp valve.

8. The blood sample quantitative dispensing device according to claim 2, characterized in that: It also includes a control system, which is electrically connected to a pressure sensor (2), a first plunger pump (3), a second plunger pump (4), a first blood sensor (5), and a second blood sensor (6).

9. A method for adding samples using a blood sample quantitative addition device, characterized in that... The blood sample quantitative addition device according to any one of claims 1 to 8 includes the following steps: S1. Close the third clamp valve (9) and the fourth clamp valve (10), and open the first clamp valve (7) and the second clamp valve (8). S2. The sample arm needle passes through the test tube cap and enters the test tube. The first plunger pump (3) runs to draw sample blood. After the sample blood flows through the first blood sensor (5), the sample arm needle lifts up. The first plunger pump (3) continues to work. The sample blood flows through the first clamp valve (7) and reaches the manifold (11). At the same time, the second plunger pump (4) starts to work. The sixth delivery tube (136) extends into the diluent storage to draw diluent. The diluent flows through the second clamp valve (8) to the second plunger pump (4), and then through the fifth delivery tube (135) and the second clamp valve (8) to be delivered to the manifold (11). It is initially mixed in the manifold (11). S3. The initially mixed sample moves upward under the action of the first plunger pump (3) and the second plunger pump (4), and reaches the second blood sensor (6) through the third delivery tube (133) and the fourth delivery tube (134). At this time, the first plunger pump (3) and the second plunger pump (4) stop working. S4. The first clamp valve (7) is closed, the third clamp valve (9) is opened, the first plunger pump (3) starts to pump back, the prepared sample flows back, through the manifold (11) and the third clamp valve (9), through the seventh delivery tube (137) and through the sample loading seat (15), and is injected into the erythrocyte sedimentation rate measuring tube through the sample loading seat (15), thus completing a quantitative sample loading process.

10. A method for cleaning a blood sample quantitative dispensing device, comprising the following steps: S1. The rotating component inside the rotating fixed stage (14) drives the sample feeding seat (15) and the extraction seat (16) to rotate, reaching the cleaning position; S2. The diaphragm pump starts to first extract the residual liquid in the device pipeline; S3. After the residue extraction is completed, the sample arm needle is placed into pure water. The diaphragm pump continues to work to extract pure water. The pure water passes through the first delivery pipe (131), the first clamp valve (7) and the manifold (11). At the same time, the first plunger pump (3) starts to work. The pure water will flow upward and pass through the third delivery pipe (133) and the fourth delivery pipe (134). S4. The diaphragm pump continues to work, and pure water passes through the manifold (11), the seventh delivery pipe (137) and the third clamp valve (9), and is discharged into the sample dosing seat (15) and the extraction seat (16), and is finally discharged through the diaphragm pump. S5. The diaphragm pump works continuously to draw pure water, and the first plunger pump (3) works synchronously to repeatedly clean the third delivery pipe (133) and the fourth delivery pipe (134) three times to complete the cleaning work.