Sample pretreatment device, and sample analysis system and sample analysis method using same

Abstract

The present invention provides a sample pretreatment device, and a sample analysis system and sample analysis method using same. The sample pretreatment device comprises: a housing having an inner space; a pretreatment unit that is accommodated in the inner space of the housing and pretreats a sample using one or more reagents; and a controller which is connected to a driving unit for providing a driving force to the pretreatment unit and controls the pretreatment of the sample. The pretreatment unit may select at least some of the sample and the one or more reagents to adjust a flow, and may mix the selected at least some to pretreat the sample.

Description

Sample pretreatment device, sample analysis system using the same, and sample analysis method

[0001] The present invention relates to a sample pretreatment device, a sample analysis system using the same, and a sample analysis method.

[0002] Intestinal microorganisms present in our bodies have a close relationship with human cells and are reported to play important roles in the human immune system, metabolic processes, and the manifestation of diseases. Furthermore, intestinal microorganisms are reported to be involved in the development and progression of various intestinal diseases—such as functional bowel disorders, irritable bowel disorders, inflammatory bowel diseases, and antibiotic-induced Clostridium difficile infections—as well as allergic diseases, autism, various metabolic diseases, diabetes, obesity, and various types of cancer. Accordingly, interest in technologies capable of collecting and analyzing intestinal microorganisms is increasing.

[0003] The present invention aims to provide a sample pretreatment device that automates the sample collection and pretreatment process, a sample analysis system using the same, and a sample analysis method.

[0004] To achieve the above objectives, one aspect of the present invention provides a sample pretreatment device comprising: a housing having an internal space; a pretreatment unit accommodated in the internal space of the housing and pretreating a sample using one or more reagents; and a controller connected to a driving unit that provides driving force to the pretreatment unit and controls the pretreatment of the sample, wherein the pretreatment unit selects at least a portion of the sample and the one or more reagents to control the flow and mixes the selected at least portion to pretreat the sample.

[0005] A sample pretreatment device, a sample analysis system using the same, and a sample analysis method according to one embodiment of the present invention automate the processes of sample collection, pretreatment, and packaging so that a sample can be obtained quickly and easily and used for analysis while minimizing sample contamination.

[0006] A sample pretreatment device, a sample analysis system using the same, and a sample analysis method according to one embodiment of the present invention can pretreat a sample in a consistent and efficient manner by introducing a sample and a reagent into a replaceable pretreatment unit and sequentially mixing the sample and the reagent by driving an injection control module and a rotation module.

[0007] FIG. 1 is a block diagram schematically illustrating a sample analysis system according to one embodiment of the present invention.

[0008] FIG. 2 is a diagram exemplarily showing the sample collection unit and sample packaging unit of FIG. 1.

[0009] FIG. 3 is a perspective view illustrating an exemplary sample pretreatment device of FIG. 1.

[0010] Figure 4 is a drawing showing the state in which a part of the housing has been removed from the sample pretreatment device of Figure 3.

[0011] Figure 5 is an exploded perspective view showing the preprocessing unit of Figure 3.

[0012] Figure 6 is a drawing showing an enlarged view of a part of Figure 4.

[0013] FIG. 7 is a block diagram conceptually showing the connection relationship between the driving unit of FIG. 4 and other components.

[0014] Figure 8 is a plan view specifically showing the kit base of Figure 5.

[0015] FIG. 9 is a perspective view specifically showing the kit base of FIG. 5.

[0016] FIGS. 10 to 14 are diagrams illustrating the process of the sample pretreatment device of FIG. 3 pretreating a sample.

[0017] FIGS. 15 to 18 are flowcharts illustrating a sample analysis method according to one embodiment of the present invention.

[0018] One aspect of the present invention provides a sample pretreatment device comprising: a housing having an internal space; a pretreatment unit accommodated in the internal space of the housing and pretreating a sample using one or more reagents; and a controller connected to a driving unit that provides driving force to the pretreatment unit and controls the pretreatment of the sample, wherein the pretreatment unit selects at least a portion of the sample and the one or more reagents to control the flow and mixes the selected at least portion to pretreat the sample.

[0019] Additionally, the pretreatment unit may comprise a kit base having a plurality of channels through which a fluid can flow and into which the sample and one or more reagents are introduced, and a rotary section to which each of the plurality of channels is connected, an injection control module having a plunger capable of linear reciprocating movement that communicates with an injection hole of the kit base, and a rotary module rotatably coupled to the rotary section of the kit base to communicate some of the plurality of channels with the injection hole.

[0020] In addition, the controller can control at least one of the injection control module and the rotation module to select a channel among the plurality of channels that communicates with the injection hole, and mix the sample and some of the one or more reagents.

[0021] In addition, the above reagents are provided in multiple quantities, and the controller can control at least one of the injection control module and the rotation module to sequentially flow the multiple reagents and mix each with the sample.

[0022] Additionally, the kit base includes an injection channel connecting the injection hole and the rotary section, a main channel connecting the main chamber into which the sample is introduced and the rotary section, and a sub channel connecting the sub chamber into which the reagent is introduced and the rotary section, and the rotary module has a rotating plate having a concavely formed connecting groove that rotates, and can connect either the main channel or the sub channel to the injection channel.

[0023] In addition, when some of the plurality of channels are connected to the injection hole by the rotation module, the plunger moves in a linear reciprocating motion and controls the internal pressure of the connected channel, thereby allowing at least some of the sample and one or more reagents to flow.

[0024] Another aspect of the present invention provides a sample analysis method comprising the steps of collecting a sample to be analyzed and providing the collected sample to a sample pretreatment device for pretreatment, wherein the step of pretreatment of the sample comprises the step of introducing the sample and one or more reagents used for pretreatment of the sample into the sample pretreatment device, and the step of controlling at least one of an injection control module and a rotation module of the sample pretreatment device to control the flow of at least some of the sample and the one or more reagents to pretreat the sample.

[0025] In addition, the sample pretreatment device comprises a rotary section into which the sample and one or more reagents are introduced and to which a plurality of channels through which fluid can flow are connected, and a kit base having an injection hole communicating with the injection control module, and the step of pretreating the sample comprises the rotary module being connected to the rotary section of the kit base and rotating to communicate some of the plurality of channels with the injection hole, and the plunger of the injection control module moving linearly back and forth to control the internal pressure of the channel communicating with the injection hole, thereby controlling the flow and mixing at least some of the sample and one or more reagents.

[0026] Additionally, the step of collecting the sample may comprise a step in which a sensor unit detects at least one of the location, size, and volume of the sample, and a step of collecting the sample by inserting a filament at a point corresponding to the location of the sample based on the information detected by the sensor unit.

[0027] In addition, the method may further include the step of wrapping the sample with a film and forming the film to seal and package the sample.

[0028] The structure and operation of the present invention will be described in detail below with reference to embodiments of the present invention illustrated in the attached drawings.

[0029] The present invention is capable of various modifications and may have various embodiments; specific embodiments are illustrated in the drawings and described in detail in the detailed description. The effects and features of the present invention, and the methods for achieving them, will become clear by referring to the embodiments described below in detail together with the drawings. However, the present invention is not limited to the embodiments disclosed below but can be implemented in various forms.

[0030] Hereinafter, embodiments of the present invention will be described in detail with reference to the attached drawings. When describing with reference to the drawings, identical or corresponding components are given the same reference numerals, and redundant descriptions thereof will be omitted.

[0031] In the following examples, singular expressions include plural expressions unless the context clearly indicates otherwise.

[0032] In the following embodiments, terms such as "include" or "have" mean that the features or components described in the specification are present, and do not preclude the possibility that one or more other features or components may be added.

[0033] Where an embodiment can be implemented differently, a specific process sequence may be performed differently from the order described. For example, two processes described consecutively may be performed substantially simultaneously or proceed in the reverse order of the description.

[0034] In the drawings, the size of components may be exaggerated or reduced for convenience of explanation. For example, the size and thickness of each component shown in the drawings are depicted arbitrarily for convenience of explanation, so the following embodiments are not necessarily limited to those illustrated.

[0035] FIG. 1 is a block diagram schematically showing a sample analysis system (1) according to one embodiment of the present invention, and FIG. 2 is a diagram exemplarily showing a sample collection unit (10) and a sample packaging unit (20) of FIG. 1.

[0036] Referring to FIGS. 1 and 2, the sample analysis system (1) may be equipped with a sample collection unit (10), a sample packaging unit (20), a controller (50), and a sample pretreatment device (100).

[0037] The sample analysis system (1) can collect a sample to be analyzed through a sample collection unit (10) and pre-process the sample through a sample pre-processing device (100). Additionally, the sample analysis system (1) can seal and package the sample through a sample packaging unit (20), and the packaged sample can be analyzed in a separate sample analysis device (not shown).

[0038] The sample collection unit (10) can collect a sample to be analyzed. Although the present specification describes an example in which the sample is feces expelled from a person's body, the type of sample to be analyzed in the present invention is not particularly limited.

[0039] The sample collection unit (10) may be equipped with a toilet (11), a sensor unit (12), a filament supply unit (13), and a filament cutting unit (14).

[0040] A sample discharged into the toilet (11) can be detected by a sensor unit (12). The sensor unit (12) can detect the location, size, volume, etc. of the sample to obtain information about the sample. For example, the sensor unit (12) may include a camera capable of 3D scanning the sample.

[0041] In one embodiment, the toilet (11) may be made of a transparent material. The sensor part (12) is placed in the toilet made of a transparent material and can detect a sample inside the toilet (11).

[0042] In one embodiment, the sensor unit (12) may be equipped with a plurality of sensors arranged radially. The sensor unit (12) can detect the sample from multiple angles to obtain more accurate information about the sample.

[0043] The controller (50) can obtain primary analysis results of feces based on information obtained from the sensor unit (12). At this time, the controller (50) can obtain primary analysis results, such as classification of feces and related findings, by using various algorithm models such as machine learning and deep learning.

[0044] The sample discharged into the toilet (11) can be collected through a filament (SF). The filament (SF) can be made of any type and form of material capable of collecting the sample.

[0045] The filament supply unit (13) can supply a filament (SF) to a point corresponding to the location of the sample based on information obtained from the sensor unit (12).

[0046] In one embodiment, the filament supply unit (13) may be provided with a reel on which a portion of the filament (SF) is wound. The filament supply unit (13) is controlled by a controller (50) so that the reel rotates and pushes the filament (SF) to a point corresponding to the position of the sample.

[0047] The filament cutting unit (14) can cut the filament (SF). When the filament (SF) is supplied to a predetermined point corresponding to the location of the sample, the sample can be collected on the filament (SF), and the filament cutting unit (14) can cut one side of the filament (SF). At this time, the filament cutting unit (14) is provided in the form of a button and can be operated by the user's button operation. Alternatively, the filament cutting unit (14) may be controlled by a controller (50) to automatically cut the filament (SF).

[0048] The sample collection unit (10) may further be provided with a gripping part (not shown). The gripping part (not shown) can grip the filament (SF) that has been collected as a sample from the toilet (11) and supply it to a sample pretreatment device (100) or a sample packaging unit (20). At this time, the gripping part (not shown) may be provided in a form such as a multi-jointed robot arm and may have all kinds of parts and devices capable of gripping and transporting the filament (SF).

[0049] In this way, the sample analysis system (1) accurately collects samples in a consistent manner through the sample collection unit (10), and can minimize contamination of the samples during the collection process.

[0050] The sample packaging unit (20) can seal and package the sample. The sample packaging unit (20) may package the sample collected from the sample collection unit (10) immediately, or may package the sample pretreated in the sample pretreatment device (100).

[0051] The sample packaging unit (20) may be equipped with a packaging device (21), a rapid kit storage unit (22), and a sample acquisition unit (23).

[0052] The packaging device (21) can seal and package the sample. Specifically, the filament (SF) from which the sample was collected can be fed into the packaging device (21) and automatically sealed. At this time, the sample may be in a state collected from the sample collection unit (10) or may be in a state pretreated by the sample pretreatment device (100).

[0053] In one embodiment, although not specifically illustrated in the drawings, the packaging device (21) can supply a packaging film surrounding a filament (SF) and form the packaging film to package the filament (SF). For example, the packaging device (21) can sequentially high-temperature press the edges of the packaging film surrounding the filament (SF) to form it into the shape of a sealed bag that forms an internal space. Additionally, the packaging device (21) can high-temperature cut the packaging film to separate the sealed filament package.

[0054] A rapid kit storage unit (22) may store a rapid kit capable of testing a sample. The rapid kit storage unit (22) may be positioned on one side of the packaging device (21) to provide the rapid kit to the packaging device (21). The rapid kit provided to the packaging device (21) may be used to simply and quickly test and diagnose the sample before or simultaneously with packaging.

[0055] The sample acquisition unit (23) can acquire a packaged sample. For example, the sample acquisition unit (23) is provided in the form of a box positioned adjacent to the packaging device (21), so that a packaged filament package can be inserted and stored.

[0056] The sample pretreatment device (100) can pretreat a sample. A sample collected from the sample collection unit (10) can be provided to the sample pretreatment device (100) for pretreatment. The sample pretreatment device (100) may be placed adjacent to the sample collection unit (10) or may be placed separately in a separate space.

[0057] Specifically, the collected sample needs to be appropriately pretreated according to the purpose, conditions, and method of analysis. For example, if the sample is feces excreted from the body, the feces need to be homogenized, diluted, and filtered in advance for immune or genetic analysis. The sample analysis system (1) can analyze the sample more quickly and efficiently by pretreating the sample through the sample pretreatment device (100).

[0058] In one embodiment, the sample analysis system (1) can be used in a space environment. The sample analysis system (1) can be equipped in a space station or the like to collect and pre-process samples for analysis. The sample analysis system (1) automates the processes of sample collection, pre-processing, and packaging, so that samples can be analyzed quickly and simply without the need for operation by a professional user.

[0059] Below, the specific configuration and pretreatment method of the sample pretreatment device (100) will be described in detail.

[0060] FIG. 3 is an exemplary perspective view of the sample pretreatment device (100) of FIG. 1, and FIG. 4 is a drawing showing the sample pretreatment device (100) of FIG. 3 with a part of the housing (110) removed.

[0061] In the drawing, the 'X-axis' and the 'Y-axis' intersecting it in the 3-axis orthogonal coordinate system are substantially defined as horizontal directions, and the 'Z-axis' is defined as vertical directions. Additionally, in an embodiment of the present invention, the 'X-axis' and the 'Y-axis' may be parallel to the placement plane where the sample pretreatment device (100) is placed, and the 'Z-axis' may be perpendicular to the placement plane. Additionally, the 'Z-axis' may correspond to the height direction of the sample pretreatment device (100).

[0062] Referring to FIGS. 3 and 4, the sample pretreatment device (100) may be equipped with a housing (110), a robot arm unit (120), and a pretreatment unit (1000).

[0063] The housing (110) can form the exterior of the sample pretreatment device (100). The housing (110) may have an internal space, and a pretreatment unit (1000) may be accommodated in the internal space.

[0064] Although not shown in the drawing, the housing (110) can be sealed by a separate cover part (not shown). Through this, the sample pretreatment device (100) can pretreat the sample in a completely sealed state.

[0065] A robot arm unit (120) may be positioned on one side of the housing (110). The robot arm unit (120) may perform physical operations such as introducing a sample and reagent into the pretreatment unit (1000) or stirring. The operation of the robot arm unit (120) may be controlled by a controller (50).

[0066] The robot arm unit (120) may be equipped with one or more links and may operate with multiple degrees of freedom. The specific form and operating principle of the robot arm unit (120) are not particularly limited and may be provided in any form that can be positioned adjacent to the preprocessing unit (1000) to perform a predetermined task.

[0067] The pretreatment unit (1000) can pretreat a sample. A sample and a reagent may be introduced into the pretreatment unit (1000), and the sample may be pretreated by controlling the flow of the sample and the reagent. The operation of the pretreatment unit (1000) can be controlled by a controller (50).

[0068] In one embodiment, the pretreatment unit (1000) may be housed within a sealed space. For example, the pretreatment unit (1000) may be housed within the internal space of a sealed housing (110) surrounded by a cover portion (not shown). Alternatively, the sample pretreatment device (100) may be placed in a sealed space separated from the outside, so that the pretreatment unit (1000) may be housed within the sealed space. In this way, the pretreatment unit (1000) can control the flow of the sample, reagent, or mixture thereof by controlling the internal pressure of the channels through the injection control module (1200), which will be described later.

[0069] FIG. 5 is an exploded perspective view showing the preprocessing unit (1000) of FIG. 3.

[0070] Referring to FIGS. 4 and FIGS. 5 together, the pretreatment unit (1000) may be equipped with a kit base (1100), an injection control module (1200), and a rotation module (1300).

[0071] The kit base (1100) may be provided with a sample to be pretreated and a reagent used to pretreat the sample. The kit base (1100) may be equipped with chambers into which the sample and the reagent are injected, respectively. Additionally, the kit base (1100) may be equipped with channels through which the sample and the reagent can flow.

[0072] An injection control module (1200) can be coupled to one side of the kit base (1100). The injection control module (1200) can flow samples and reagents by controlling the internal pressure of the channels provided in the kit base (1100).

[0073] Figure 6 is a drawing showing an enlarged view of a part of Figure 4.

[0074] Referring to FIGS. 5 and 6, the injection control module (1200) may be equipped with a coupling syringe (1210) and a plunger (1220).

[0075] A coupling syringe (1210) can be coupled to a kit base (1100). An injection hole (1110) capable of communicating with channels is formed on one side of the kit base (1100), and the coupling syringe (1210) can be coupled to the injection hole (1110). The coupling syringe (1210) may have a hollow that communicates with the injection hole (1110). The coupling syringe (1210) may be supported by a support portion (1120) of the kit base (1100).

[0076] The plunger (1220) can be coupled to the coupling syringe (1210) to enable linear reciprocating motion. The plunger (1220) is coupled to the hollow of the coupling syringe (1210) and can move linearly by receiving driving force from the driving unit (130) to be described later. As the plunger (1220) moves linearly, the internal pressure of the channel forming a flow path in communication with the injection hole (1110) can be regulated. As a result, a sample, reagent, or a mixture thereof can flow in the channel.

[0077] The plunger (1220) may be provided with a meshing gear (1221) on one side. The meshing gear (1221) of the plunger (1220) may be arranged to mesh with the driving gear (131g) of the first driving module (131) to be described later. As a result, the plunger (1220) can perform linear reciprocating motion by the rotation of the driving gear (131g).

[0078] The operation of the injection control module (1200) can be controlled by a controller (50). The controller (50) can control the direction of movement and the speed of movement of the plunger (1220) by controlling the output of the first drive module (131), which will be described later. Through this, the type, flow rate, and flow velocity of the material flowing in the kit base (1100) can be controlled.

[0079] The rotation module (1300) is coupled to the kit base (1100) and can rotate. The kit base (1100) may be provided with a plurality of channels through which a sample and one or more reagents can flow, respectively. The rotation module (1300) can rotate and control the flow of the sample or reagent to selectively flow only through some of the plurality of channels.

[0080] The rotation module (1300) may be equipped with a rotatable rotation plate (1310). The rotation plate (1310) is connected to a second drive module (132) to be described later, and can rotate by receiving a driving force. The rotation plate (1310) may be detachably provided to a fixed part (132h) of the second drive module (132).

[0081] The rotating plate (1310) can be connected to the rotary portion (not shown) of the kit base (1100) to be described later. Additionally, the second driving module (132) and the kit base (1100) can be coupled by a coupling member (132f).

[0082] In this way, the kit base (1100) and the injection control module (1200) and rotation module (1300) connected thereto can be interchangeably provided within the sample pretreatment device (100). By doing so, the accuracy and reliability of the pretreatment results can be ensured by pretreating each sample using a separate pretreatment unit (1000).

[0083] In one embodiment, a sealing member (1320) may be placed between a rotating plate (1310) and a rotary section (not shown). The area where the rotating module (1300) and the kit base (1100) are joined can be sealed by the sealing member (1320), and a sample or reagent can flow smoothly in the chamber and channel formed inside the kit base (1100).

[0084] The rotating plate (1310) is provided with a predetermined plate shape, and a connecting groove (OP) formed concavely on one side may be disposed therein. The position of the connecting groove (OP) can be adjusted according to the rotation of the rotating plate (1310). A channel connected at a point corresponding to the position of the connecting groove (OP) communicates with an injection control module (1200) to regulate internal pressure. As a result, a sample, reagent, or a mixture thereof may flow along the channel.

[0085] The operation of the rotation module (1300) can be controlled by a controller (50). The controller (50) can adjust the rotation direction, rotation angle, etc. of the rotation plate (1310) by adjusting the output of the second drive module (132), which will be described later. Through this, a channel through which fluid can flow can be selected.

[0086] The principle of flow control for samples, reagents, etc., according to the operation of the rotation module (1300), and the method of sample pretreatment by the pretreatment unit (1000) will be explained in detail below.

[0087] The sample pretreatment device (100) may further be equipped with a driving unit (130). The driving unit may provide the power required for the pretreatment of the sample.

[0088] FIG. 7 is a block diagram conceptually showing the connection relationship between the driving unit (130) of FIG. 4 and other configurations.

[0089] Referring to FIG. 7, the driving unit (130) can provide driving force to at least one of the robot arm unit (120) and the preprocessing unit (1000). The driving unit (130) is controlled by a controller (50) to transmit driving force to the robot arm unit (120) or the preprocessing unit (1000).

[0090] The drive unit (130) may be equipped with a power source capable of generating power, such as a motor or cylinder, and may be equipped with a power transmission structure such as a gear or shaft capable of transmitting power generated from the power source. The detailed structure of the drive unit (130) and the method of power generation and transmission are not particularly limited.

[0091] The driving unit (130) may be equipped with a first driving module (131), a second driving module (132), a third driving module (133), and a fourth driving module (134).

[0092] The first driving module (131) can provide driving force to the injection control module (1200) of the pretreatment unit (1000). The first driving module (131) is connected to the injection control module (1200) and can transmit driving force to enable the injection control module (1200) to operate to control the flow of the sample and reagent.

[0093] As shown in FIG. 6, the first drive module (131) may be equipped with a rotating shaft (131a) and a drive gear (131g) coupled thereto. As the drive gear (131g) coupled to the rotating shaft (131a) rotates, a plunger (1220) having a meshing gear (1221) engaged with the drive gear (131g) can perform linear reciprocating motion. Through this, the injection control module (1200) operates and can control the flow of a sample, reagent, etc.

[0094] The second driving module (132) can provide driving force to the rotation module (1300) of the pretreatment unit (1000). The second driving module (132) is connected to the rotation module (1300), and as the rotation plate (1310) of the rotation module (1300) rotates, the internal pressure is controlled by the injection control module (1200), so that a channel through which a sample, reagent, etc. can flow can be selected.

[0095] The third driving module (133) can provide a driving force for controlling magnetic particles in the reagent. Magnetic particles may be administered into the reagent for the pretreatment of a sample, and the sample may be pretreated through the control of the magnetic particles. For example, in an example where feces is the sample, magnetic particles in the reagent may be precipitated for the purification of nucleic acids. The third driving module (133) can provide a driving force for controlling these magnetic particles.

[0096] In detail, the sample pretreatment device (100) may be equipped with a position-adjustable magnetic part (not shown), and a third driving module (133) may provide driving force to the magnetic part (not shown). As a result, the position of the magnetic part (not shown) may be adjusted so as to be adjacent to the reagent to which magnetic particles are administered.

[0097] The fourth driving module (134) can provide a driving force for fluorescence detection. The sample pretreatment device (100) can detect a fluorescent substance in the sample itself or in a mixture of the sample and a reagent to perform sample pretreatment and accompanying sample analysis. For example, in an embodiment where feces is the sample, fluorescence detection can be performed to detect intestinal microorganisms, etc.

[0098] The sample pretreatment device (100) may further be equipped with optical components, such as a light source, for detecting fluorescent substances. At this time, the position of the optical components may be adjusted as needed. The fourth driving module (134) may provide driving force to the optical components, and as a result, detection of fluorescent substances may be achieved in the target sample or in a mixture of the sample and a reagent.

[0099] The drive unit (130) may further be equipped with a fifth drive module (not shown) that provides driving force to the robot arm unit (120). The robot arm unit (120) operates by receiving driving force from the fifth drive module (not shown) and can perform tasks such as introducing a sample or reagent into a chamber, stirring, or other pretreatment operations. At this time, parts for introduction, stirring, etc., may be attached to the robot arm unit (120).

[0100] Below, the detailed structure of the kit base (1100) and the sample pretreatment method according to it are described.

[0101] FIG. 8 is a plan view specifically showing the kit base (1100) of FIG. 5, and FIG. 9 is a perspective view specifically showing the kit base (1100) of FIG. 5.

[0102] Referring to FIGS. 8 and 9, the pretreatment unit (1000) may be equipped with a plurality of chambers. A sample and a reagent may be injected into each chamber.

[0103] In detail, a sample and one or more reagents may be injected into the kit base (1100) of the pretreatment unit (1000). The chamber into which the sample is injected is defined as the main chamber (MB), and the chamber into which the reagent is injected is defined as the sub-chamber (SB). At this time, the number of sub-chambers (SB) may correspond to the number of reagents injected into the pretreatment unit. That is, different substances may be contained in each chamber.

[0104] Hereinafter, for convenience of explanation, the description will focus on an embodiment in which four sub-chambers (SB) are provided in the kit base (1100) as shown in FIGS. 8 and 9, and each will be defined as the first sub-chamber (SB1), the second sub-chamber (SB2), the third sub-chamber (SB3), and the fourth sub-chamber (SB4). Different types of reagents may be injected into the first sub-chamber (SB1) to the fourth sub-chamber (SB4).

[0105] The main chamber (MB), the first sub-chamber (SB1) to the fourth sub-chamber (SB4) may be spaced apart from each other within the kit base (1100) and may be formed into a predetermined space into which a sample or reagent can be injected.

[0106] The pretreatment unit (1000) may have a plurality of channels. A sample, a reagent, or a mixture thereof may flow through each channel.

[0107] In detail, a plurality of channels through which fluid can flow may be formed in the kit base (1100) of the pretreatment unit (1000). Each channel extends from the injection hole (1110) or the main chamber (MB) or sub-chamber (SB) and may be provided in the form of a passage through which fluid can flow.

[0108] The pretreatment unit (1000) may be equipped with a rotary section (RT). A rotary module (1300) may be coupled to the rotary section (RT), and a plurality of channels may be connected to each. Depending on the rotation of the rotary plate (1310) connected to the rotary section (RT), a channel among the plurality of channels through which fluid can flow may be selected.

[0109] A connecting groove (OP) formed concavely in the rotating plate (1310) can have one end fixedly positioned at the center (RC) of the rotary section (RT). As the rotating plate (1310) rotates, the connecting groove (OP) can rotate about the center (RC) of the rotary section (RT) as an axis.

[0110] The pretreatment unit (1000) may be provided with an injection channel (CC). An injection hole (1110) communicating with an injection control module (1200) is formed on one side of the kit base (1100), and the injection channel (CC) may extend from the injection hole (1110). The injection channel (CC) may connect the injection hole (1110) with a rotary unit (RT).

[0111] The pretreatment unit (1000) may be equipped with a main channel (MC). The main channel (MC) may extend from a main chamber (MB) into which a sample is injected and be connected to a rotary section (RT).

[0112] In one embodiment, the main channel (MC) may include a main first channel section (MCa) extending from the main chamber (MB) and a main second channel section (MCb) connecting the main first channel section (MCa) and the rotary section (RT). The heights at which the main first channel section (MCa) and the main second channel section (MCb) are positioned on the kit base (1100) may differ from each other.

[0113] For convenience of explanation, among the outer surfaces of the kit base (1100), the surface located relatively lower with respect to the 'Z-axis' of FIG. 9 is defined as the upper surface, and the surface located relatively higher is defined as the upper surface.

[0114] As shown in FIG. 9, the main first channel section (MCa) may be formed adjacent to the lower surface of the kit base (1100), and the main second channel section (MCb) may be formed adjacent to the upper surface of the kit base while extending from the main first channel section (MCa). The main channel (MC) is formed with a structure having a predetermined step, so that when the channel through which fluid flows is changed according to the position adjustment of the connecting groove (OP), the position in which the solution flowing through the main channel (MC) is received can be adjusted.

[0115] For example, when the connecting groove (OP) rotates to connect the sub-channel (SC) and the injection channel (CC) while the sample or reagent mixture is extracted from the main chamber (MB), the extracted material may be contained in the main second channel section (MCb). Through this, even when the injection control module (1200) and the main channel (MC) are not connected, a predetermined amount of material extracted from the main chamber (MB) can remain in the main channel (MC). Through this, the timing of injection and the amount of the material being injected or extracted can be precisely controlled.

[0116] The pretreatment unit (1000) may have a first sub-channel (SC1), a second sub-channel (SC2), a third sub-channel (SC3), and a fourth sub-channel (SC4). The first sub-channel (SC1) to the fourth sub-channel (SC4) may each extend from the first sub-chamber (SB1) to the fourth sub-chamber (SB4) and be connected to a rotary unit (RT).

[0117] One end of each injection channel (CC), main channel (MC), and first sub-channel (SC1) to fourth sub-channel (SC4) can be connected to the rotary section (RT). One end of the injection channel (CC) can be connected to the center (RC) of the rotary section (RT). The main channel (MC) and the first sub-channel (SC1) to fourth sub-channel (SC4) can be spaced apart from the center (RC) of the rotary section (RT) by the same distance.

[0118] The position of the connecting groove (OP) can be changed by the rotation of the rotating plate (1310). When the position of the connecting groove (OP) is adjusted, the injection channel (CC) is connected to any one of the main channel (MC), the first sub-channel (SC1) to the fourth sub-channel (SC4) in a single flow path so that fluid can flow. The injection control module (1200) can allow the sample, reagent, or a mixture thereof to flow by controlling the pressure within the connected flow path.

[0119] In one embodiment, the first sub-channel (SC1) may include a first sub-channel section (SC1a) extending from the first sub-chamber (SB1) and a first sub-channel section (SC1b) connecting the first sub-channel section (SC1a) and the rotary section (RT). The heights of the first sub-channel section (SC1a) and the first sub-channel section (SC1b) in the kit base (1100) may differ from each other. Similar to the main channel (MC), the first sub-channel section (SC1a) may be formed adjacent to the lower surface of the kit base (1100), and the first sub-channel section (SC1b) may be formed adjacent to the upper surface of the kit base (1100).

[0120] Likewise, each of the second sub-channel (SC2), the third sub-channel (SC3), and the fourth sub-channel (SC4) may be provided with a second sub-first channel section (SC2a) and a second sub-second channel section (SC2b), a third sub-first channel section (SC3a) and a third sub-second channel section (SC3b), and a fourth sub-first channel section (SC4a) and a fourth sub-second channel section (SC4b) arranged at different heights from each other.

[0121] Each sub-channel (SC) is formed with a structure having a predetermined step, so that when the fluid flow channel changes according to the position adjustment of the connecting groove (OP), the position in which the solution flowing through the sub-channel (SC) is received can be adjusted.

[0122] For example, when the connecting groove (OP) is rotated to connect the injection channel (CC) to the main channel (MC) or any one of the second sub-channel (SC2) to the fourth sub-channel (SC4) while the mixture with the reagent or sample is extracted in the first sub-chamber (SB1), the extracted substance may be contained in the first sub-second channel section (SC1b). Through this, even when the injection control module (1200) and the first sub-channel (SC1) are not connected, a predetermined amount of substance extracted from the first sub-chamber (SB1) can remain within the first sub-channel (SC1). Through this, the timing of injection and the amount of the substance being injected or extracted can be precisely controlled.

[0123] In one embodiment, the injection channel (CC), main channel (MC), and sub channel (SC) may each be formed inside the kit base (1100). Alternatively, at least a portion of the injection channel (CC), main channel (MC), and sub channel (SC) may be exposed on the upper surface of the kit base (1100), as shown in FIG. 5. The pretreatment unit (1000) is placed in a sealed space so that fluid can flow in each channel by driving the injection control module (1200).

[0124] In one embodiment, the kit base (1100) may further have a plurality of insertion holes. Depending on the purpose of pretreatment, the process of performing pretreatment, etc., components such as filtration filters and vials may be additionally inserted into the insertion holes. FIGS. 8 and 9 illustrate an embodiment in which a first insertion hole (OP1) is provided on a first sub-channel (SC1) of the kit base (1100), and a second insertion hole (OP2) and a third insertion hole (OP3) are provided on a separate channel extending from the rotary section (RT).

[0125] Next, the specific process of the sample pretreatment device (100) pretreating the sample is described, focusing on the detailed structure of the kit base (1100).

[0126] For convenience of explanation, the following description focuses on the sample pretreatment process obtained by lysing cells contained in feces, followed by purifying and washing the nucleic acid. However, as mentioned above, various sample pretreatment operations may be performed depending on the type of sample and reagent introduced into the pretreatment unit (1000).

[0127] FIGS. 10 to 14 are drawings exemplarily illustrating the process of the sample pretreatment device (100) of FIG. 3 pretreating a sample (SP).

[0128] In FIGS. 10 to 14, the reagents introduced into the first sub-chamber (SB1), second sub-chamber (SB2), third sub-chamber (SB3), and fourth sub-chamber (SB4) of the kit base (1100), respectively, are defined as the first reagent (TS1), second reagent (TS2), third reagent (TS3), and fourth reagent (TS4).

[0129] At this time, the first reagent (TS1) may be a buffer solution capable of homogenizing the fecal sample. Additionally, the second reagent (TS2) may be provided as a reagent capable of lysing cells in the feces, such as intestinal microorganisms. Additionally, the third reagent (TS3) may be provided as a reagent capable of purifying nucleic acids from the lysed cells, and the fourth reagent (TS4) may be provided as a reagent capable of washing the purified nucleic acids.

[0130] Additionally, for convenience of explanation in FIGS. 10 to 14, the rotation module (1300) is conceptually illustrated only with the connecting groove (OP), and other components are omitted.

[0131] Referring together to FIGS. 8 to 14, the sample pretreatment device (100) can pretreat the sample by sequentially extracting or injecting the sample, reagent, or mixture thereof.

[0132] FIG. 10 shows the state in which a sample pretreatment device (100) extracts a first reagent (TS1).

[0133] A single flow path can be formed by rotating the connecting groove (OP) of the rotation module (1300) to connect the injection channel (CC) and the first sub-channel (SC1). Subsequently, as the plunger (1220) of the injection control module (1200) moves in the first direction (D1), the first reagent (TS1) can be extracted by flowing from the first sub-chamber (SB1) along the first sub-channel (SC1) and the injection channel (CC).

[0134] FIG. 11 shows a state in which a sample pretreatment device (100) injects the extracted first reagent (TS1) into the main chamber (MB) where the sample is placed, or extracts a substance from the main chamber (MB).

[0135] A single flow path can be formed by rotating the connecting groove (OP) of the rotating module (1300) to connect the injection channel (CC) and the main channel (MC). Subsequently, as the plunger (1220) of the injection control module (1200) moves in the second direction (D2), the extracted first reagent (TS1) can flow along the injection channel (CC) and the main channel (MC) and be injected into the main chamber (MB).

[0136] When the first reagent (TS1) is injected into the main chamber (MB), the sample (SP) can be dissolved and homogenized in the first reagent (TS1). At this time, the robot arm unit (120) may be controlled by the controller (50) to stir the sample (SP) and the first reagent (TS1). The solution produced by dissolving the sample (SP) in the first reagent (TS1) is defined as the sample solution.

[0137] When the sample (SP) is dissolved in the first reagent (TS1), the plunger (1220) of the injection control module (1200) moves in the first direction (D1) so that the sample solution can be extracted. The sample solution can flow from the main chamber (MB) along the main channel (MC) and the injection channel (CC).

[0138] FIG. 12 shows a state in which a sample pretreatment device (100) injects the extracted sample solution into a second sub-chamber (SB2) in which a second reagent (TS2) is placed, or extracts a substance from the second sub-chamber (SB2).

[0139] A single flow path can be formed by rotating the connecting groove (OP) of the rotation module (1300) to connect the injection channel (CC) and the second sub-channel (SC2). Subsequently, as the plunger (1220) of the injection control module (1200) moves in the second direction (D2), the extracted sample solution can flow along the injection channel (CC) and the second sub-channel (SC2) and be injected into the second sub-chamber.

[0140] When the sample lysis solution is injected into the second sub-chamber (SB2), the second reagent (TS2) and the sample lysis solution are mixed, and the cells contained in the sample may be lysed, and the resulting solution is defined as the cell lysis solution.

[0141] When the sample lysate is mixed with the second reagent (TS2), the plunger (1220) of the injection control module (1200) moves in the first direction (D1) so that the cell lysate can be extracted. The cell lysate can flow from the second sub-chamber (SB2) along the second sub-channel (SC2) and the injection channel (CC).

[0142] FIG. 13 shows a state in which a sample pretreatment device (100) injects the extracted cell lysate into a third sub-chamber (SB3) in which a third reagent (TS3) is placed, or extracts a substance from the third sub-chamber (SB3).

[0143] A single flow path can be formed by rotating the connecting groove (OP) of the rotation module (1300) to connect the injection channel (CC) and the third sub-channel (SC3). Subsequently, as the plunger (1220) of the injection control module (1200) moves in the second direction (D2), the extracted cell lysate can flow along the injection channel (CC) and the third sub-channel (SC3) and be injected into the third sub-chamber (SB3).

[0144] When cell lysate is injected into the third sub-chamber (SB3), the third reagent (TS3) and cell lysate are mixed, and nucleic acids within the cells can be purified. The resulting solution is defined as the nucleic acid purification solution.

[0145] In one embodiment, the third reagent (TS3) may contain magnetic particles (not shown) used for the purification of nucleic acids. Nucleic acids contained in the cell lysate may be adsorbed onto the magnetic particles (not shown), and the nucleic acids may be purified as the magnetic particles (not shown) settle. Accordingly, the sample pretreatment device (100) may move the magnetic part (MG) to be adjacent to the third sub-chamber (SB3) by means of the third driving module (133). The magnetic part (MG) positioned adjacent to the third sub-chamber (SB3) may cause the magnetic particles (not shown) to settle, thereby allowing the nucleic acids to be highly purified.

[0146] When the cell lysate is mixed with the third reagent (TS3), the plunger (1220) of the injection control module (1200) moves in the first direction (D1) so that the nucleic acid purification solution can be extracted. The nucleic acid purification solution can flow from the third sub-chamber (SB3) along the third sub-channel (SC3) and the injection channel (CC).

[0147] FIG. 14 shows a state in which a sample pretreatment device (100) injects the extracted nucleic acid purification solution into a fourth sub-chamber (SB4) in which a fourth reagent (TS4) is placed, or extracts a substance from the fourth sub-chamber (SB4).

[0148] A single flow path can be formed by rotating the connecting groove (OP) of the rotation module (1300) to connect the injection channel (CC) and the fourth sub-channel (SC4). Subsequently, as the plunger (1220) of the injection control module (1200) moves in the second direction (D2), the extracted nucleic acid purification solution can flow along the injection channel (CC) and the fourth sub-channel (SC4) and be injected into the fourth sub-chamber (SB4).

[0149] When the nucleic acid purification solution is injected into the fourth sub-chamber (SB4), the fourth reagent (TS4) and the nucleic acid purification solution are mixed, and the purified nucleic acid can be washed. The resulting solution is defined as the nucleic acid washing solution.

[0150] When the nucleic acid purification solution is mixed with the fourth reagent (TS4), the plunger (1220) of the injection control module (1200) moves in the first direction (D1) to extract the nucleic acid washing solution. The nucleic acid washing solution can flow from the fourth sub-chamber (SB4) along the fourth sub-channel (SC4) and the injection channel (CC). The nucleic acid obtained in this way can be provided to a sample analysis device (not shown) to perform immunoassay, gene analysis, etc.

[0151] In this way, the sample pretreatment device (100) can control the injection control module (1200) and the rotation module (1300) to extract or inject the sample, reagent, or mixture thereof introduced into each chamber. In particular, a specific chamber and a channel extending therefrom can be selectively connected to the injection control module (1200) by the operation of the rotation module (1300), and as a result, the sample can be pretreated sequentially.

[0152] All or part of the sample pretreatment process shown in FIGS. 10 to 14 may be repeated a predetermined number of times, and as a result, all collected samples may be pretreated. In addition, once the sample pretreatment is completed, the pretreated sample is extracted, and the pretreatment unit used may be discarded. In this way, the pretreatment unit is provided to be replaceable, so that pretreatment can be performed in a consistent manner and environment for each sample to be pretreated.

[0153] FIGS. 15 to 18 are flowcharts illustrating a sample analysis method according to one embodiment of the present invention.

[0154] Referring to FIGS. 15 to 18, the sample analysis method may include the step of collecting a sample to be analyzed (S100), the step of providing the collected sample to a sample pretreatment device for pretreatment (S200), and the step of sealing and packaging the pretreated sample (S300).

[0155] In the step of collecting the sample to be analyzed (S100), the sample to be analyzed can be obtained. In an embodiment where the sample is feces, the step of collecting the sample to be analyzed (S100) can detect and collect the sample discharged into the toilet.

[0156] The step of collecting a sample to be analyzed (S100) may include a step (S110) in which a sensor unit detects at least one of the location, size, and volume of the sample, and a step (S120) of collecting a sample by inserting a filament at a point corresponding to the location of the sample.

[0157] The step (S110) in which the sensor unit detects at least one of the location, size, and volume of the sample allows the sensor unit to acquire information about the sample. The sensor unit can acquire information about the sample by detecting the location, size, volume, etc. of the sample.

[0158] The step (S120) of collecting a sample by inserting a filament at a point corresponding to the location of the sample can collect a sample using a filament based on information obtained from the sensor unit. A filament capable of collecting a sample can be inserted at a point corresponding to the location of the sample, and when a sample is collected on the filament, one side of the filament can be cut to obtain the sample.

[0159] The step (S200) of providing the collected sample to a sample pretreatment device for pretreatment may involve pretreating the sample in a sample pretreatment device. Depending on the purpose of analysis, analysis conditions, etc., the sample may be pretreated in a sample pretreatment device before analysis.

[0160] The step (S200) of providing a collected sample to a sample pretreatment device for pretreatment may include the step (S210) of introducing a sample and one or more reagents used for the analysis of the sample into the sample pretreatment device, and the step (S220) of controlling at least one of an injection control module and a rotation module of the sample pretreatment device to control the flow of the sample, reagent, or a mixture thereof to pretreat the sample.

[0161] The step (S210) of introducing a sample and one or more reagents used for the analysis of the sample into a sample pretreatment device may involve introducing the sample and the reagents into the sample pretreatment device. The sample may be introduced into the main chamber of the sample pretreatment device, and the reagents into the sub-chamber.

[0162] The step (S220) of pre-treating a sample by controlling the flow of a sample, reagent, or mixture thereof by controlling at least one of the injection control module and the rotation module of the sample pre-treatment device can pre-treat the sample by controlling at least one of the injection control module and the rotation module.

[0163] The sample pretreatment device may have multiple channels through which a sample, reagent, or a mixture thereof can flow, and any one of the multiple channels may be connected to an injection control module depending on the operation of a rotary module. The injection control module can regulate the internal pressure of the connected channel to allow the sample, reagent, mixture, etc. to flow. When a sample and multiple reagents are introduced into the sample pretreatment device, the sample can be pretreated by sequentially mixing with each reagent.

[0164] In an embodiment where the sample is feces, the specific pretreatment method of the step (S220) of pretreating the sample by controlling the flow of the sample, reagent, or mixture thereof by controlling at least one of the injection control module and the rotation module of the sample pretreatment device is as follows.

[0165] The step (S220) of pre-treating a sample by controlling at least one of the injection control module and the rotation module of the sample pre-treatment device to control the flow of the sample, reagent, or mixture thereof is as follows: connecting an injection hole to a first sub-chamber into which a first reagent is introduced to extract the first reagent (S221); connecting an injection hole to a main chamber into which a sample is introduced to inject the extracted first reagent into the main chamber (S222); when the sample is dissolved in the first reagent, extracting the sample lysis solution (S223); connecting an injection hole to a second sub-chamber into which a second reagent is introduced to inject the extracted sample lysis solution into the second sub-chamber (S224); when cells contained in the sample are dissolved by the second reagent, extracting the cell lysis solution (S225); connecting an injection hole to a third sub-chamber into which a third reagent is introduced to inject the extracted cell lysis solution into the third sub-chamber (S226); and when nucleic acid is purified from the cell lysis solution by the third reagent, extracting the nucleic acid purification solution. The method may include a step (S227), a step (S228) of connecting the fourth sub-chamber into which the fourth reagent has been introduced and the injection hole to inject the extracted nucleic acid purification solution into the fourth sub-chamber, and a step (S229) of obtaining a nucleic acid washing solution containing nucleic acid washed by the fourth reagent.

[0166] The step (S221) of extracting the first reagent by connecting the first sub-chamber into which the first reagent is introduced and the injection hole can be performed by driving a rotary module to form a single flow path connecting the first sub-chamber and the injection hole, and by controlling the internal pressure of the flow path using an injection control module to extract the first reagent. At this time, the first reagent may be provided as a reagent capable of dissolving and homogenizing a sample.

[0167] The step (S222) of connecting the main chamber into which the sample is introduced and the injection hole to inject the extracted first reagent into the main chamber can be achieved by driving a rotary module to form a single flow path connecting the main chamber and the injection hole, and by controlling the internal pressure of the flow path using an injection control module to inject the extracted first reagent into the main chamber. Through this, the first reagent and the sample are mixed within the main chamber, and the sample can be dissolved and homogenized.

[0168] When the sample is dissolved in the first reagent, the step of extracting the sample solution (S223) allows the sample solution to be extracted from the main chamber by operating the injection control module while the main chamber and the injection hole are connected.

[0169] The step (S224) of connecting the second sub-chamber into which the second reagent is introduced and the injection hole to inject the extracted sample solution into the second sub-chamber can be achieved by driving a rotary module to form a single flow path connecting the second sub-chamber and the injection hole, and by controlling the internal pressure of the flow path using an injection control module to inject the extracted sample solution into the second sub-chamber.

[0170] The second reagent introduced into the second sub-chamber may be provided as a reagent capable of lysing the cells of the sample. As a result, the second reagent and the sample lysis solution are mixed within the second sub-chamber, and the cells of the sample can be lysed.

[0171] When the cells contained in the sample are lysed by the second reagent, the step of extracting the cell lysate (S225) allows the cell lysate to be extracted from the second sub-chamber by driving the injection control module while the second sub-chamber and the injection hole are connected.

[0172] The step (S226) of injecting the extracted cell lysate into the third sub-chamber by connecting the third sub-chamber into which the third reagent has been introduced and the injection hole is formed by driving a rotation module to form a single flow path connecting the third sub-chamber and the injection hole, and the injection control module can inject the extracted cell lysate into the third sub-chamber by controlling the internal pressure of the flow path.

[0173] The third reagent introduced into the third sub-chamber may be equipped as a reagent capable of purifying nucleic acids within the cell. As a result, the third reagent and the cell lysate are mixed within the third sub-chamber, and the nucleic acids can be purified.

[0174] The step (S226) of injecting the extracted cell lysate into the third sub-chamber by connecting the third sub-chamber into which the third reagent has been introduced and the injection hole can be adjusted so as to be adjacent to the third sub-chamber. The third reagent introduced into the third sub-chamber may contain magnetic particles for the purification of nucleic acids. By positioning the magnetic part at a location adjacent to the third sub-chamber, the nucleic acids can be highly purified as the magnetic particles settle.

[0175] When nucleic acid is purified from the cell lysate by the third reagent, the step of extracting the nucleic acid purified solution (S227) can be performed by driving the injection control module while the third sub-chamber and the injection hole are connected, so that the nucleic acid purified solution can be extracted from the third sub-chamber.

[0176] The step (S228) of connecting the fourth sub-chamber into which the fourth reagent has been introduced and the injection hole to inject the extracted nucleic acid purification solution into the fourth sub-chamber can be achieved by driving a rotation module to form a single flow path connecting the fourth sub-chamber and the injection hole, and by controlling the internal pressure of the flow path using an injection control module to inject the extracted nucleic acid purification solution into the fourth sub-chamber.

[0177] The fourth reagent introduced into the fourth sub-chamber may be provided as a reagent capable of washing purified nucleic acid. As a result, the fourth reagent and the nucleic acid purification solution are mixed within the fourth sub-chamber, and the nucleic acid can be washed.

[0178] The step (S229) of obtaining a nucleic acid washing solution containing nucleic acid washed by the fourth reagent can be performed by driving an injection control module while the fourth sub-chamber and the injection hole are connected, thereby extracting the nucleic acid washing solution from the fourth sub-chamber. The sample pretreatment device can obtain the extracted nucleic acid washing solution through sequential pretreatment steps, and the nucleic acid washing solution can be provided to a separate sample analysis device and used for immunoassay, genetic analysis, etc. of the sample.

[0179] In this way, the step (S200) of providing the collected sample to a sample pretreatment device for pretreatment can be performed by introducing the sample and one or more reagents into the sample pretreatment device and mixing the sample with the reagents. At this time, by controlling the operation of the injection control module and the rotation module of the sample pretreatment device, the sample can be mixed with one or more reagents and pretreated sequentially.

[0180] The step (S300) of sealing and packaging the pre-processed sample may involve wrapping the sample with a film and forming the film to seal and package the sample. The film for packaging the sample may be formed into a sealed bag that contains the sample and has a sealed internal space through processes such as high-temperature pressing or high-temperature cutting. The sample sealed and packaged in this manner may be stored in a separate storage unit or provided to a sample analysis device for use in analysis.

[0181] Although not shown in the drawing, the sample analysis method may further include a step of providing the collected sample to a sample analysis device for analysis. In this case, the sample may be provided to the sample analysis device immediately after collection, or it may be provided to the sample analysis device in a pre-processed and sealed packaged state.

[0182] Meanwhile, the order of each step of the sample analysis method shown in FIGS. 15 to 18 is exemplary, and each step may be performed simultaneously or the order may differ. In addition, all or some steps of the sample analysis method shown in FIGS. 15 to 18 may be repeated a predetermined number of times, thereby obtaining a precisely pre-processed sample. Furthermore, regarding specific sample collection, pre-processing methods, and detailed structures of the sample pre-processing device for each step, reference will be made to the descriptions in FIGS. 1 to 14.

[0183] A sample pretreatment device according to one embodiment of the present invention, a sample analysis system including the same, and a sample analysis method automate the collection, pretreatment, and packaging of samples to minimize sample contamination and enable rapid and simple acquisition and analysis of samples.

[0184] A sample pretreatment device, a sample analysis system including the same, and a sample analysis method according to one embodiment of the present invention are equipped with a replaceable pretreatment unit, allowing for convenient pretreatment operations to be performed for each sample. A sample pretreatment device, a sample analysis system including the same, and a sample analysis method according to one embodiment of the present invention control the flow of the sample and reagent by driving an injection control module and a rotation module of the pretreatment unit, thereby enabling sample pretreatment in a consistent manner and ensuring the reliability and accuracy of the pretreatment results.

[0185] As such, the present invention has been described with reference to the embodiments illustrated in the drawings, but this is merely illustrative, and those skilled in the art will understand that various modifications and equivalent alternative embodiments are possible therefrom. Accordingly, the true technical scope of protection of the present invention should be determined by the technical spirit of the appended claims.

[0186] The specific practices described in the embodiments are examples and do not limit the scope of the embodiments in any way. Furthermore, unless specifically stated as "essential," "importantly," etc., components may not be strictly necessary for the application of the present invention.

[0187] In the specification of the embodiments (particularly in the claims), the use of the term "the above" and similar descriptive terms may be in both singular and plural. Furthermore, where a range is described in the embodiments, it is considered to include the invention with respect to individual values ​​within said range (unless otherwise stated), and is equivalent to describing each individual value constituting said range in the detailed description. Finally, regarding the steps constituting the method according to the embodiments, unless explicitly stated in order or otherwise stated, said steps may be performed in a suitable order. The embodiments are not necessarily limited by the order in which said steps are described. The use of all examples or exemplary terms (e.g., etc.) in the embodiments is merely for the purpose of describing the embodiments in detail, and the scope of the embodiments is not limited by said examples or exemplary terms unless limited by the claims. Furthermore, those skilled in the art will understand that various modifications, combinations, and changes may be made according to design conditions and factors within the scope of the claims or equivalents.

[0188] According to one embodiment of the present invention, a sample pretreatment device, a sample analysis system using the same, and a sample analysis method are provided. Furthermore, embodiments of the present invention can be applied to techniques for pretreating and analyzing samples, etc.

Claims

1. Housing having an internal space; A pretreatment unit accommodated in the internal space of the above-mentioned housing and pretreating a sample using one or more reagents; and A controller connected to a driving unit that provides driving force to the above-mentioned pretreatment unit and controls the pretreatment of the sample; comprising The above preprocessing unit is, A sample pretreatment device that selects at least some of the above sample and at least one of the above reagents to control the flow, and mixes the selected at least some to pretreat the above sample.

2. In Paragraph 1, The above preprocessing unit is, A kit base having a plurality of channels through which fluid can flow, into which the above sample and the above one or more reagents are introduced, and a rotary section to which the plurality of channels are each connected; An injection control module communicating with the injection hole of the above-mentioned kit base and having a linearly reciprocating plunger; and A sample pretreatment device comprising: a rotary module rotatably coupled to the rotary portion of the kit base and communicating some of the plurality of channels with the injection hole.

3. In Paragraph 2, The above controller is, A sample pretreatment device that controls at least one of the injection control module and the rotation module to select a channel communicating with the injection hole among the plurality of channels and mixes the sample and some of the one or more reagents.

4. In Paragraph 2, The above reagents are provided in multiple quantities, and The above controller is, A sample pretreatment device that controls at least one of the injection control module and the rotation module to sequentially flow the plurality of reagents and mix them with the sample.

5. In Paragraph 2, The above kit base is, An injection channel connecting the injection hole and the rotary section; A main channel connecting the main chamber into which the above sample is introduced and the rotary section; and It includes a sub-channel connecting the sub-chamber into which the above-mentioned reagent is introduced and the above-mentioned rotary section; and The above-mentioned rotating module is, A sample pretreatment device having a rotating plate having a concavely formed connecting groove that rotates and connects either the main channel and the sub-channel to the injection channel.

6. In Paragraph 2, The above injection control module is, A sample pretreatment device in which, when some of the plurality of channels are connected to the injection hole by the above-described rotating module, the plunger moves linearly back and forth to regulate the internal pressure of the connected channels, thereby causing the sample and at least some of the one or more reagents to flow.

7. A step of collecting a sample to be analyzed; and The method includes the step of providing the collected sample to a sample pretreatment device for pretreatment; The step of pre-treating the above sample is, A step of introducing the above sample and one or more reagents used for the pretreatment of the above sample into the above sample pretreatment device; and A sample analysis method comprising the step of pre-treating the sample by controlling at least one of the injection control module and the rotation module of the sample pretreatment device to control the flow of at least some of the sample and one or more reagents.

8. In Paragraph 7, The above sample pretreatment device is, A kit base having a rotary section to which a plurality of channels through which fluid can flow are connected, into which the above sample and the above one or more reagents are introduced, and an injection hole communicating with the above injection control module; The step of pre-treating the above sample is, The above-mentioned rotating module is connected to the rotary part of the kit base and rotates, and some of the plurality of channels are connected to the injection hole, and A sample analysis method in which a plunger of the injection control module moves linearly back and forth to control the internal pressure of a channel communicating with the injection hole, thereby controlling the flow and mixing of at least some of the sample and one or more reagents.

9. In Paragraph 7, The step of collecting the above sample is, A step in which a sensor unit detects at least one of the position, size, and volume of the sample; and A sample analysis method comprising the step of collecting the sample by inserting a filament at a point corresponding to the location of the sample based on information detected by the sensor unit.

10. In Paragraph 7, A sample analysis method further comprising the step of wrapping the sample with a film and forming the film to seal and package the sample.

Images