Systems and methods for interwoven functional capillary arrays

Abstract

An assembly can include a plurality of capillaries. The plurality of capillaries can include a first capillary and a second capillary. The assembly can include a first fiber woven between the first capillary and the second capillary, around a portion of the first capillary, and around a portion of the second capillary. The portion of the first capillary and the portion of the second capillary can be parallel. A first portion of the first fiber and a second portion of the first fiber can be parallel. A detector can be configured to couple with a portion of the plurality of capillaries between a first end of each of the plurality of capillaries and a second end of each of the plurality of capillaries. The second end of each of the plurality of capillaries can receive a sample.

Description

Atty. Dkt. No. 20210012-01 (130073-0120) SYSTEMS AND METHODS FOR INTERWOVEN FUNCTIONAL CAPILLARY ARRAYSTECHNICAL FIELD

[0001] The present application relates generally to capillary electrophoresis.BACKGROUND

[0002] Capillary electrophoresis (CE) can be used to assess the size, purity, and composition of samples by separating analytes (e.g., ions) in a capillary. The analytes can migrate through the capillary using an applied electric field. The analytes can be separated based on their electrophoretic mobility.SUMMARY

[0003] Assembling capillary arrays by hand can be a time-consuming process due to complex routing requirements and the potential to damage the individual capillaries. The solutions described herein can provide an interwoven capillary array that includes capillaries woven with fibers (e.g., functional fibers, tubing, shielding, wires). The fibers can provide mechanical support to the capillary array, heating capabilities, cooling capabilities, control of optical properties such as avoiding crosstalk between neighboring capillaries, and / or optical excitation or emission detection. The fibers can be selected so that they reflect, absorb or allow transmission in certain wavelength ranges, depending on the application. This allows a wide variety of optical effects to be achieved.

[0004] At least one aspect of the present disclosure is directed to an assembly. The assembly can include a plurality of capillaries. The plurality of capillaries can include a first capillary and a second capillary. The assembly can include a first fiber woven between the first capillary and the second capillary, around a portion of the first capillary, and around a portion of the second capillary. The portion of the first capillary and the portion of the second capillaryAtty. Dkt. No. 20210012-01 (130073-0120) can be parallel. A first portion of the first fiber and a second portion of the first fiber can be parallel. A detector can be configured to couple with a portion of the plurality of capillaries between a first end of each of the plurality of capillaries and a second end of each of the plurality of capillaries. The second end of each of the plurality of capillaries can receive a sample.

[0005] Another aspect of the present disclosure is directed to an assembly. The assembly can include a plurality of capillaries arranged in a braided configuration. A detector can be configured to couple with a portion of the plurality of capillaries between a first end of each of the plurality of capillaries and a second end of each of the plurality of capillaries. The second end of each of the plurality of capillaries can receive a sample.

[0006] Another aspect of the present disclosure is directed to a method. The method can include weaving a first fiber between a first capillary of a plurality of capillaries and a second capillary of the plurality of capillaries, around a portion of the first capillary, and around a portion of the second capillary. The method can include positioning the portion of the first capillary parallel to the portion of the second capillary. The method can include positioning a first portion of the first fiber parallel to a second portion of the first fiber. A detector can be configured to couple with a portion of the plurality of capillaries between a first end of each of the plurality of capillaries and a second end of each of the plurality of capillaries. The second end of each of the plurality of capillaries can receive a sample.

[0007] Those skilled in the art will appreciate that the summary is illustrative only and is not intended to be in any way limiting. Other aspects, inventive features, and advantages of the devices and / or processes described herein, as defined solely by the claims, will become apparent in the detailed description set forth herein and taken in conjunction with the accompanying drawings.BRIEF DESCRIPTION OF THE DRAWINGSAtty. Dkt. No. 20210012-01 (130073-0120)

[0008] The details of one or more implementations of the subject matter described in this specification are set forth in the accompanying drawings and the description below. Other features, aspects, and advantages of the subject matter will become apparent from the description, the drawings, and the claims.

[0009] FIG. l is a diagram of a capillary electrophoresis system, in accordance with an embodiment.

[0010] FIG. 2 is a diagram of an assembly, in accordance with an embodiment.

[0011] FIG. 3 is a diagram of the assembly, in accordance with an embodiment.

[0012] FIG. 4 is a diagram of a cross-section of the assembly, in accordance with an embodiment.

[0013] FIG. 5 is a diagram of weaving patterns, in accordance with an embodiment.

[0014] FIG. 6 is a view of the assembly, in accordance with an embodiment.

[0015] FIG. 7 is a view of the assembly, in accordance with an embodiment.

[0016] Like reference numbers and designations in the various drawings indicate like elements.DETAILED DESCRIPTION

[0017] Following below are more detailed descriptions of various concepts related to, and implementations of interwoven functional capillary arrays. The various concepts introduced above and discussed in greater detail below may be implemented in any of a number of ways, as the described concepts are not limited to any particular manner of implementation. Examples of specific implementations and applications are provided primarily for illustrative purposes.

[0018] Assembling capillary arrays by hand can be a time-consuming process. For example, it may take more than 8 hours to build a capillary array having 96 capillaries. The design of capillary arrays can be limited to those based on manual manufacturability. Due toAtty. Dkt. No. 20210012-01 (130073-0120) manual assembly steps, the capillary arrays can require high tolerances, be less reproducible from array to array, and have a higher error potential. High tolerances refers to the issue that the spacing between capillaries and / or the diameters of through-holes in capillary holder structures (e.g., comb) need to be designed in a way to make manual assembly of the capillary array possible. Manual assembly or fabrication of the array can increase the risk of breaking or damaging the capillaries compared to automated methods. Damages to the array may not be recognized before the array is completed and tested. In some cases, the entire array must be scrapped due to damaged capillaries. Capillary to capillary deviation due to the manual assembly process can be an issue. Additionally, heat dissipation using forced air flow can lead to flow gradients, which can result in temperature gradients and inhomogeneous effects from capillary to capillary. For example, areas where capillaries are more tightly spaced can have higher flow resistance and therefore are cooled less effectively. Uniform or controlled thermostatting by air flow alone is a challenge. Furthermore, compact routing is difficult due to capillary elasticity and accessibility for manual labor (e.g., sequentially threading each capillary).

[0019] Embodiments of the present disclosure describe interwoven functional capillary arrays. The solutions described herein can provide an interwoven capillary array fabric that includes a mixture of capillaries and other functional fibers or structures (e.g., tubing, shielding, wires) to implement active and / or passive mechanical, optical, or thermal features and that interface with the compact interwoven capillary array. The solutions described herein can prevent undesirable temperature gradients of the capillary array due to air flow gradients.

[0020] The disclosed solutions have a technical advantage of providing temperature regulation (e.g., cooling, heating) of the capillaries. The solutions can provide spatially resolved cooling or heating. The capillaries can be interwoven with other materials to implement mechanical, optical, and / or heat transitional properties to the interwoven capillaryAtty. Dkt. No. 20210012-01 (130073-0120) array. The functional materials can be parallel or perpendicular to the capillaries. The solutions can eliminate crosstalk using opaque separators that are interwoven between the capillaries. The solutions can provide for optical excitation or emission detection via interwoven excitation / emission fibers. The solutions can provide mixed weaving of capillaries and functional fibers or channels to generate interwoven capillary array fabrics in an automated, precise, and reproducible manner. The solutions can provide for a highly compact, densely packed, and / or homogenous array of capillaries that is independent of the length of the capillaries.

[0021] FIG. 1 is a diagram of a capillary electrophoresis system 100 (e.g., capillary electrophoresis system, CE system, CE device). The CE system 100 can include a representative CE system. The CE system 100 can include a CE instrument. The CE instrument can include components (e.g., fluidic components). The CE system 100 can include a fluidic system. The CE system 100 can be used for high-throughput electrophoresis applications. For example, the CE system 100 can be used for high-throughput parallel capillary electrophoresis applications.

[0022] The CE system 100 can include one or more capillaries. The CE system 100 can include a plurality of capillaries 105 (e.g., capillary bundle, separation capillaries). Although only one capillary of the plurality of capillaries 105 is shown in FIG. 1, the CE system 100 can include any number of capillaries. For example, the plurality of capillaries 105 can include 12 capillaries. The plurality of capillaries 105 can include between 48 and 96 capillaries. The plurality of capillaries 105 can include up to 384 capillaries. For example, the CE system 100 can include between 1 and 384 capillaries. Each capillary of the plurality of capillaries 105 can include a fused-silica capillary. For example, each capillary of the plurality of capillaries 105 can include a narrow-bore, fused-silica capillary. The plurality of capillaries 105 can be made of fused-silica or other materials. Each capillary of the plurality of capillaries 105 can have aAtty. Dkt. No. 20210012-01 (130073-0120) length. The length of each capillary of the plurality of capillaries 105 can be greater than 50 cm (e.g., 88 cm).

[0023] The CE system 100 can include an outlet reservoir 110. The outlet reservoir 110 can be filled with a solution (e.g., buffer solution, liquid). The outlet reservoir 110 can be emptied of the solution. The outlet reservoir 110 can be cleaned by flowing a cleaning solution into the outlet reservoir 110.

[0024] The outlet reservoir 110 can be fluidically coupled with (e.g., connected to, attached to) the plurality of capillaries 105. For example, a first end 106 of each of the plurality of capillaries 105 can be fluidically coupled with the outlet reservoir 110. The plurality of capillaries 105 can be inserted into the outlet reservoir 110. For example, the plurality of capillaries 105 can be inserted into (e.g., submerged in, disposed in) the solution disposed in the outlet reservoir 110. The plurality of capillaries 105 can be inserted into the outlet reservoir 110 before the outlet reservoir 110 is filled with the solution. The outlet reservoir 110 can be filled with the solution subsequent to the plurality of capillaries 105 being disposed in the outlet reservoir 110. A plurality of tips (e.g., capillary tips) of the first end 106 of each of the plurality of capillaries 105 can be inserted into the solution disposed in the outlet reservoir 110.

[0025] The CE system 100 can include an inlet reservoir 115. The inlet reservoir 115 can be filled with a solution (e.g., buffer solution). The inlet reservoir 115 can be fluidically coupled with the plurality of capillaries 105. For example, a second end 107 of each of the plurality of capillaries 105 can be fluidically coupled with the inlet reservoir 115. The second end 107 of each of the plurality of capillaries 105 can be opposite the first end 106 of each of the plurality of capillaries 105. The plurality of capillaries 105 can be inserted into the inlet reservoir 115. For example, the plurality of capillaries 105 can be inserted into the solution disposed in the inlet reservoir 115. The plurality of capillary tips of the second end 107 of each of the plurality of capillaries 105 can be inserted into the solution disposed in the inlet reservoirAtty. Dkt. No. 20210012-01 (130073-0120) 115. The inlet reservoir 115 can be moved towards the plurality of capillaries 105 such that the second end 107 of each of the plurality of capillaries 105 fluidically couples with the inlet reservoir 115.

[0026] The CE system 100 can include one or more sample trays 120. The sample tray 120 can include a plurality of wells. For example, the sample tray 120 can include a 96-well sample tray. Each well of the sample tray may contain a liquid sample. The sample can include, for example, proteins (e.g., antibodies, enzymes), peptides, polynucleotides (e.g., DNA, RNA), and / or oligonucleotides. The sample tray 120 can move to the location of the inlet reservoir 115. For example, the sample tray 120 can take the place of the inlet reservoir 115. The sample tray 120 can be fluidically coupled with the plurality of capillaries 105. For example, the second end 107 of each of the plurality of capillaries 105 can be fluidically coupled with the sample tray 120. The plurality of capillaries 105 can be inserted into the sample tray 120. For example, the plurality of capillaries 105 can be inserted into samples disposed in the sample tray 120. The sample tray 120 can include a plurality of wells. Each capillary of the plurality of capillaries 105 can be loaded with a different sample. The plurality of capillary tips of the second end 107 of each of the plurality of capillaries 105 can be inserted into the samples disposed in the sample tray 120. The sample tray 120 can be moved towards the plurality of capillaries 105 such that the second end 107 of each of the plurality of capillaries 105 fluidically couples with samples disposed in the sample tray 120.

[0027] The CE system 100 can include one or more electrodes 125. The one or more electrodes 125 can be disposed in the outlet reservoir 110. The one or more electrodes 125 can be oriented vertically in the outlet reservoir 110. The one or more electrodes 125 can be electrically coupled with the solution disposed in the outlet reservoir 110. The one or more electrodes 125 can be disposed in the inlet reservoir 115. The one or more electrodes 125 can be electrically coupled with the solution disposed in the inlet reservoir 115. The one or moreAtty. Dkt. No. 20210012-01 (130073-0120) electrodes 125 can electrically couple the outlet reservoir 110 to the inlet reservoir 115. The one or more electrodes 125 can be electrically coupled with the solution disposed in the outlet reservoir 110. The one or more electrodes 125 can be electrically coupled with the plurality of capillaries 105.

[0028] The CE system 100 can include one or more power supplies 130 (e.g., power source). The power supply 130 can include a source of power to an electronic circuit. The one or more electrodes 125 can electrically couple the plurality of capillaries 105 with the power supply 130. The power supply 130 can provide power to move the sample tray 120. The power supply 130 can be used to drive motors. The power supply 130 can be used to for electrophoresis. The power supply 130 can include a high-voltage (HV) power supply. The power supply 130 can provide power to move the inlet reservoir 115. The power supply 130 can provide power to pump a solution (e.g., gel, sieving gel) into the plurality of capillaries 105. The power supply 130 can provide power to electrokinetically inject samples into the plurality of capillaries 105.

[0029] The CE system 100 can include one or more detectors 135. The detector 135 can include a CCD (charged-coupled device) camera or a CMOS (complementary metal-oxide semiconductor) camera. The detector 135 can detect the samples and its fractions during and / or after separation while the sample and its fractions are migrating through the plurality of capillaries. The samples can be separated according to their electrophoretic mobility inside the plurality of capillaries 105 and be detected by the detector 135. The electrophoretic mobility of the samples can be influenced by hydrodynamic radius, charge, and / or viscosity of the sample media.

[0030] The CE system 100 can include one or more controllers 140. The controller 140 can be communicably connected, directly or indirectly, to the detector 135, the power supply 130, and / or other components of the CE system 100. The controller 140 can be electricallyAtty. Dkt. No. 20210012-01 (130073-0120) coupled with the CE system 100. The controller 140 can be an onboard computing component that is physically incorporated into a housing of the CE system 100. The controller 140 can be one or more separate computing devices and / or other such controlling devices that are internal and / or external to the housing of the CE system 100. The controller 140 or a portion of the controller 140 can reside within the CE system 100. For example, the controller 140 or a portion of the controller 140 can be disposed in the CE system 100. The controller 140 can be disposed outside of the CE system 100.

[0031] The controller 140 can include one or more processors, such as but not limited to, a single-core processor, a multi-core processor, a logic device, or other such data processing circuitry, configured to execute, analyze, and process data and information of the CE system 100. The controller 140 can include a non-transitory memory device communicably connected to the processor. The memory device may be configured as a volatile memory device (e.g., SRAM and DRAM), a non-volatile memory device (e.g., flash memory, ROM, and hard disk drive), or any combination thereof. The memory device may store executable code and other such information that is generated and / or processed by the processor during operation of the CE system 100.

[0032] The CE system 100 can include one or more input / output devices communicably connected to the controller 140. The input / output device can enable an operator and / or user to receive information from the controller 140 and to input information and parameters into the controller 140. Such information and parameters can be stored in the memory device, accessed by the processor, and output to the input / output device. For example, the input / output device can include a monitor, display device, touchscreen device, keyboard, microphone, joystick, dial, button, or other such device to enable input and output of information and parameters. The input / output device may be utilized to input information intoAtty. Dkt. No. 20210012-01 (130073-0120) the controller 140 and output or otherwise display information and data generated by the processor of the CE system 100.

[0033] FIG. 2 is a diagram of an assembly 200. The assembly 200 can include a capillary array (e.g., functional capillary array, interwoven capillary array, interwoven functional capillary array, interwoven capillary array structure). The interwoven structure can allow for movement of the single capillaries of the plurality of capillaries 105, which can allow them to move relative to their surroundings and reduce internal stress when bending. The interwoven structure can provide for flexible routing, mounting, and / or fixation. The interwoven structure can be mass producible on machinery, such as a loom designed to accommodate capillaries. The interwoven capillary array can be used for capillary electrophoresis, sequencing, and / or gas chromatography applications. Braiding or other textile manufacturing methods can be used to form mixed fabric capillary arrays and to tailor properties to application needs (e.g., mechanical, optical, thermal, etc.).

[0034] The assembly 200 can include the plurality of capillaries 105. The plurality of capillaries 105 can include two or more capillaries. In one example, the plurality of capillaries 105 includes a first capillary 205 and a second capillary 210. In another example, the plurality of capillaries 105 includes the first capillary 205, the second capillary 210, and a third capillary 215. The assembly 200 can include more than three capillaries (e.g., 12 capillaries, 48 capillaries, 96 capillaries, 384 capillaries).

[0035] The assembly 200 can include one or more fibers. For example, the one or more fibers can include a first fiber 220. In another example, the one or more fibers includes the first fiber 220 and a second fiber 225. In yet another example, the one or more fibers includes the first fiber 220, the second fiber 225, and a third fiber 230. The assembly 200 can include more than three fibers (e.g., four fibers, five fibers, six fibers, etc.). The one or more fibers can holdAtty. Dkt. No. 20210012-01 (130073-0120) the plurality of capillaries 105 in a fixed position. The one or more fibers can mechanically strengthen the assembly 200.

[0036] The fiber of the one or more fibers can include a tube (e.g., micro tubing, hollow tube). The tube can circulate a liquid. For example, a liquid can be circulated inside of the tube. The tube can include an enclosed circuit (e.g., enclosed heating circuit, enclosed cooling circuit). The tube can have an inlet and an outlet that can be used to circulate the liquid. Each circuit can be isolated from other circuits to allow for independent temperature control of each circuit.

[0037] The fiber of the one or more fibers can include (e.g., be made of) at least one of metal or alloy (e.g. copper or stainless steel), glass, polymer, or thread (e.g., clothing fibers such as wool). For example, the first fiber 220, the second fiber 225, and the third fiber 230 can be made of at least one of metal or alloy (e.g. copper or stainless steel), glass, polymer, or thread. The first fiber 220, the second fiber 225, and the third fiber 230 can be made of the same material or different materials. The one or more fibers can include a wire (e.g., resistance heating wire, mechanical guide wire). The mechanical guide wire can optimize routing by forcing the interwoven capillary array into a particular shape (e.g., mat configuration, cylindrical configuration). The one or more fibers can include an optical fiber for detection (e.g. for providing excitation light or for collecting light such as light transmitted through a capillary and maybe attenuated by a sample in the capillary or fluorescence light emitted from a sample in the capillary).

[0038] The first fiber 220 can be woven between the first capillary 205 and the second capillary 210. For example, a portion of the first fiber 220 can be disposed between the first capillary 205 and the second capillary 210. The first fiber 220 can hold the first capillary 205 and the second capillary 210 together.Atty. Dkt. No. 20210012-01 (130073-0120)

[0039] The first fiber 220 can be woven around a portion of the first capillary 205 (e.g., first capillary first portion 235). For example, the first fiber 220 can be wrapped around the first capillary first portion 235. The first fiber 220 can be adjacent to the first capillary first portion 235. The portion of the first fiber 220 disposed between the first capillary 205 and the second capillary 210 can be continuous with the portion of the first fiber 220 disposed around the first capillary first portion 235.

[0040] The first fiber 220 can be woven around a portion of the second capillary 210 (e.g., second capillary first portion 240). For example, the first fiber 220 can be wrapped around the second capillary first portion 240. The first fiber 220 can be adjacent to the second capillary first portion 240. The portion of the first fiber 220 disposed between the first capillary 205 and the second capillary 210 can be continuous with the portion of the first fiber 220 disposed around the second capillary first portion 240.

[0041] In some embodiments, the second capillary 210 can be disposed between the first capillary 205 and the third capillary 215. The first fiber 220 can be woven between the second capillary 210 and the third capillary 215. The first fiber 220 can be woven around a portion of the third capillary 215.

[0042] The portion of the first capillary 205 and the portion of the second capillary 210 can be parallel. For example, the first capillary first portion 235 and the second capillary first portion 240 can be parallel. At least one section of the first capillary 205 and at least one section of the second capillary 210 can be parallel. The portion of the first capillary 205 and the portion of the third capillary 215 can be parallel. The portion of the second capillary 210 and the portion of the third capillary 215 can be parallel. At least one section of the first capillary 205 and at least one section of the third capillary 215 can be parallel. At least one section of the second capillary 210 and at least one section of the third capillary 215 can be parallel.Atty. Dkt. No. 20210012-01 (130073-0120)

[0043] A first portion of the first fiber 220 (e.g., first fiber first portion 245) and a second portion of the first fiber 220 (e.g., first fiber second portion 250) can be parallel. For example, the first fiber first portion 245 can be parallel to the first fiber second portion 250. The first fiber first portion 245 can be separated from the first fiber second portion 250 by a distance greater than, less than, or equal to a diameter (e.g., width, thickness) of the first fiber 220. The first fiber first portion 245 can be adjacent to the first fiber second portion 250. A distance between the first fiber first portion 245 and the first fiber second portion 250 can be greater than, less than, or equal to the diameter of the first fiber 220.

[0044] The portion of the first capillary 205 and the portion of the second capillary 210 can be perpendicular to the first portion of the first fiber 220 and the second portion of the first fiber 220. For example, the first capillary first portion 235 can be perpendicular to the first fiber first portion 245. The first capillary first portion 235 can be perpendicular to the first fiber second portion 250. The second capillary first portion 240 can be perpendicular to the first fiber first portion 245. The second capillary first portion 240 can be perpendicular to the first fiber second portion 250.

[0045] In some embodiments, the first capillary first portion 235 is not perpendicular to the first fiber first portion 245 and the first fiber second portion 250. For example, the angle between the first capillary first portion 235 and the first fiber first portion 245 or the first fiber second portion 250 can be less than or greater than 90 degrees. In some embodiments, the second capillary first portion 240 is not perpendicular to the first fiber first portion 245 and the first fiber second portion 250. For example, the angle between the second capillary first portion 240 and the first fiber first portion 245 or the first fiber second portion 250 can be less than or greater than 90 degrees.

[0046] A distance between the first capillary 205 and the second capillary 210 can be greater than, less than, or equal to the diameter of the first fiber 220. For example, the distanceAtty. Dkt. No. 20210012-01 (130073-0120) between the first capillary first portion 235 and the second capillary first portion 240 can be greater than, less than, or equal to the diameter of the first fiber 220. The plurality of capillaries 105 can be tightly packed. For example, the distance between the first capillary 205 and the second capillary 210 can be equal to the diameter of the first fiber 220. The distance between the second capillary 210 and the third capillary 215 can be equal to the diameter of the first fiber 220.

[0047] In some embodiments, the distance between the plurality of capillaries 105 can be less than the diameter of the first fiber 220. For example, the first capillary 205 and the second capillary 210 can be adjacent, and the distance between the second capillary 210 and the third capillary 215 is equal to the diameter of the first fiber 220. In another example, the second capillary 210 and the third capillary 215 are adjacent, and the distance between the first capillary 205 and the second capillary 210 is equal to the diameter of the first fiber 220.

[0048] The detector 135 can be coupled (e.g., optically coupled) with a portion of the plurality of capillaries 105 between the first end 106 of each of the plurality of capillaries 105 and the second end 107 of each of the plurality of capillaries 105. For example, the detector 135 can receive one or more signals from the portion of the plurality of capillaries 105 between the first end 106 of each of the plurality of capillaries 105 and the second end 107 of each of the plurality of capillaries 105. An optical beam (e.g., light beam) can be focused on the portion of the plurality of capillaries 105 between the first end 106 of each of the plurality of capillaries 105 and the second end 107 of each of the plurality of capillaries 105. The signal from the interaction between the optical beam and the sample inside the portion of the plurality of capillaries 105 between the first end 106 of each of the plurality of capillaries 105 and the second end 107 of each of the plurality of capillaries 105 can be received by the detector 135.

[0049] In an example, the detector 135 is disposed (e.g., located) after at ’A or3 / 4 of the length of each of the plurality of capillaries 105. In another example, the portion of the pluralityAtty. Dkt. No. 20210012-01 (130073-0120) of capillaries 105 between the first end 106 of each of the plurality of capillaries 105 and the second end 107 of each of the plurality of capillaries 105 is disposed halfway between the first end 106 of each of the plurality of capillaries 105 and the second end 107 of each of the plurality of capillaries 105.

[0050] The second end 107 of each of the plurality of capillaries 105 can receive a sample. For example, the second end 107 of each of the plurality of capillaries 105 can receive the sample from the sample tray 120. The sample can include, for example, proteins, peptides, polynucleotides, and / or oligonucleotides.

[0051] The second fiber 225 can be disposed a first distance from the second end 107 of each of the plurality of capillaries 105. The first fiber 220 can be disposed a second distance from the second end 107 of each of the plurality of capillaries 105. In one example, the first distance is greater than the second distance. In another example, the first distance is less than the second distance.

[0052] The second fiber 225 can be woven around a first capillary second portion 237. The second fiber 225 can be adjacent to the first capillary second portion 237. The second fiber 225 can be woven around a second capillary second portion 242. The second fiber 225 can be adjacent to the second capillary second portion 242.

[0053] The one or more fibers can include one or more functional fibers. Functional fibers can include fibers that perform a function such as heating, cooling, or emitting light. The one or more fibers can provide spatially resolved cooling or heating in the weaving perpendicular to the plurality of capillaries 105 in the interwoven array. The cooling or heating can be accomplished, for example, via microtubing, a heat conductive wire, or resistance heating. The one or more fibers can provide a temperature gradient to the plurality of capillaries 105. The one or more fibers can include a tube. The liquid can be circulated in the tube to heat or cool the plurality of capillaries 105.Atty. Dkt. No. 20210012-01 (130073-0120)

[0054] The one or more fibers can heat (e.g., increase the temperature of) each capillary of the plurality of capillaries 105. The one or more fibers can be made of a wire, such as a resistance heating wire, to heat up each capillary of the plurality of capillaries. Heating the capillary can increase the temperature of the substance inside the capillary. For example, the first fiber 220 can heat each capillary of the plurality of capillaries 105. The first fiber 220 can heat the first capillary first portion 235. The first fiber 220 can heat the second capillary first portion 240. The first fiber 220 can heat the first capillary first portion 235 and the second capillary first portion 240 to the same temperature. In some embodiments, the second fiber 225 can heat the first capillary second portion 237. The second fiber 225 can heat the second capillary second portion 242. The second fiber 225 can heat the first capillary second portion 237 and the second capillary second portion 242 to the same temperature.

[0055] For example, the first fiber 220 can heat the first capillary first portion 235 to a first temperature. The first fiber 220 can heat the second capillary first portion 240 to the first temperature. The first fiber 220 can maintain the temperature of the first capillary first portion 235 and the second capillary first portion 240 at the same temperature (e.g., first temperature). The second fiber 225 can heat the first capillary second portion 237 to a second temperature. The second fiber 225 can heat the second capillary second portion 242 to the second temperature. The first temperature can be less than, greater than, or equal to the second temperature. The second fiber 225 can maintain the temperature of the first capillary second portion 237 and the second capillary second portion 242 at the same temperature (e.g., second temperature). The third fiber 230 can heat a first capillary third portion to a third temperature. The third fiber 230 can heat a second capillary third portion to the third second temperature. The third temperature can be less than, greater than, or equal to the second temperature. The third temperature can be less than, greater than, or equal to the first temperature. The third fiberAtty. Dkt. No. 20210012-01 (130073-0120) 230 can maintain the temperature of the first capillary third portion and the second capillary third portion at the same temperature (e.g., third temperature).

[0056] The one or more fibers can cool (e.g., decrease the temperature of) each capillary of the plurality of capillaries 105. Cooling the capillary can decrease the temperature of the substance inside the capillary. For example, the first fiber 220 can cool each capillary of the plurality of capillaries 105. The first fiber 220 can include a tube. The tube can be configured to circulate a liquid (e.g., cooling liquid). For example, the liquid can be circulated inside of the tube. The liquid circulating inside of the tube can cool each capillary of the plurality of capillaries 105. The first fiber 220 can cool the first capillary first portion 235. The first fiber 220 can cool the second capillary first portion 240. The first fiber 220 can cool the first capillary first portion 235 and the second capillary first portion 240 to the same temperature. In some embodiments, the second fiber 225 can cool the first capillary second portion 237. The second fiber 225 can cool the second capillary second portion 242. The second fiber 225 can cool the first capillary second portion 237 and the second capillary second portion 242 to the same temperature.

[0057] For example, the first fiber 220 can cool the first capillary first portion 235 to a fourth temperature. The first fiber 220 can cool the second capillary first portion 240 to the fourth temperature. The first fiber 220 can maintain the temperature of the first capillary first portion 235 and the second capillary first portion 240 at the same temperature (e.g., fourth temperature). The second fiber 225 can cool the first capillary second portion 237 to a fifth temperature. The second fiber 225 can cool the second capillary second portion 242 to the fifth temperature. The fourth temperature can be less than, greater than, or equal to the fifth temperature. The second fiber 225 can maintain the temperature of the first capillary second portion 237 and the second capillary second portion 242 at the same temperature (e.g., fifth temperature). The third fiber 230 can cool the first capillary third portion to a sixth temperature.Atty. Dkt. No. 20210012-01 (130073-0120) The third fiber 230 can cool the second capillary third portion to the sixth second temperature. The sixth temperature can be less than, greater than, or equal to the fifth temperature. The sixth temperature can be less than, greater than, or equal to the fourth temperature. The third fiber 230 can maintain the temperature of the first capillary third portion and the second capillary third portion at the same temperature (e.g., sixth temperature).

[0058] The one or more fibers can modify the temperature of each capillary of the plurality of capillaries 105. For example, the one or more fibers can include a tube (e.g., tubing) configured to circulate a liquid. The liquid which flows or is circulated inside the tube can heat or cool each capillary of the plurality of capillaries 105. The liquid can be thermostatized by an external heater or cooler. The external heater or cooler can include one or more Peltier elements (e.g., thermoelectric heater or cooler), an electric heater, a compression cooler, or other device. The liquid can be heated or cooled in an external reservoir or tank, and from there, supplied into the tubing and back to the reservoir or tank using, for example, a pump (e.g., peristaltic pump) or other method for conveying liquids.

[0059] The one or more fibers can emit light to each capillary of the plurality of capillaries 105. The light emitted to the capillary can include excitation light that excites of the sample while migrating through capillary. The dye in the sample can fluoresce and the light emitted can be collected, for example, by the detector 135 to identify the analytes. The light emitted to each capillary of the plurality of capillaries 105 can have one or more wavelengths. For example, the first fiber 220 can emit light to each capillary of the plurality of capillaries 105. The first fiber 220 can emit light to the first capillary first portion 235. The first fiber 220 can emit light to the second capillary first portion 240. The first fiber 220 emit light to the first capillary first portion 235 and the second capillary first portion 240 with the same wavelength. In some embodiments, the second fiber 225 can emit light to the first capillary second portion 237. The second fiber 225 can emit light to the second capillary second portion 242. The secondAtty. Dkt. No. 20210012-01 (130073-0120) fiber 225 can emit light to the first capillary second portion 237 and the second capillary second portion 242 with the same wavelength. The one or more fibers can irradiate the plurality of capillaries 105. The one or more fibers can include emission fibers and / or excitation fibers.

[0060] The first fiber 220 can emit light with a first wavelength to the first capillary first portion 235. The first fiber 220 can emit light with the first wavelength to the second capillary first portion 240. The second fiber 225 can emit light with a second wavelength to the first capillary second portion 237. The second fiber 225 can emit light with the second wavelength to the second capillary second portion 242. The first wavelength can be less than, greater than, or equal to the second wavelength.

[0061] The light emitted by the one or more fibers can be generated by a light source (e.g., tunable light source). The tunable light source can include a lamp (e.g., xenon lamp, HgXe lamp, deuterium lamp). The tunable light source can include a lamp with a monochromator. The light can be coupled into the one or more fibers at the terminal of the one or more fibers. The one or more fibers can include side-glowing fibers, side-firing fibers, or side-emitting fibers.

[0062] The one or more fibers can include one or more optical fibers. The one or more optical fibers can collect fluorescence emission light. The fluorescence emission light can then propagate along the one or more optical fibers. The fluorescence emission light can then be detected by a photosensitive element (e.g., photodiode, photomultiplier tube), such as a detector which is coupled to the ends of the one or more optical fibers.

[0063] In some embodiments, the temperature of the plurality of capillaries 105 can vary along the length of each capillary of the plurality of capillaries 105. For example, the temperature of the plurality of capillaries 105 can be in a range between 10°C and 70°C (e.g., between 25°C and 50°C). In another example, the first fiber 220 can heat the first capillary first portion 235 and the second capillary first portion 240 to a temperature of 70°C. In this example,Atty. Dkt. No. 20210012-01 (130073-0120) the second fiber 225 cools the first capillary second portion 237 and the second capillary second portion 242 to a temperature of 50°C. In this example, the third fiber 230 heats the first capillary third portion and the second capillary third portion to a temperature of 65°C. The temperatures that the plurality of capillaries 105 are heated or cooled to can depend on the particular application. For example, some applications can subject the plurality of capillaries 105 to heating or cooling cycles. The assembly 200 can be used for sequencing and / or quality inspection.

[0064] The assembly 200 can include a plurality of barriers (e.g., physical barriers, separators, shielding fiber). The plurality of barriers can reduce or eliminate crosstalk (e.g., when a sample inside a first capillary emits light and a second capillary next to the first capillary reflects the light and produces ghost peaks). For example, the plurality of barriers can be used to avoid crosstalk between neighboring capillaries. The plurality of barriers can include a plurality of optical shielding fibers. The plurality of barriers can reflect, absorb, or allow transmission in certain wavelength ranges. For example, the plurality of optical shielding fibers can be selected so that they reflect, absorb, or allow transmission in certain wavelength ranges depending on the application. This allows for a wide variety of optical effects to be achieved. The plurality of barriers can include a plurality of optically opaque barriers. The plurality of barriers can include a plurality of opaque separators. The plurality of optically opaque barriers can prevent light from one capillary of the plurality of capillaries 105 from reaching another capillary of the plurality of capillaries 105. The width of the barrier can be greater than, less than, or equal to the width of the capillary. The plurality of barriers can include flexible, ductile wire that can be bent to hold the plurality of capillaries 105 in a particular position. Specific wavelength dependent reflective, absorbing, or transmission optimized materials (e.g., coatings) can be used to control the optical properties of the plurality of barriers. The coatings can be used to improve excitation or eliminate certain unpreferred wavelengths. The coatingAtty. Dkt. No. 20210012-01 (130073-0120) can be applied to the plurality of barriers. For example, the coating can be applied to a portion of each barrier of the plurality of barriers. The plurality of barriers can be made of a material with certain optical properties. The coating can be applied to the plurality of capillaries 105 or parts thereof. For example, the coating can be applied to each capillary of the plurality of capillaries 105. A reflective coating can be applied to the backside of the capillary relative to the direction of excitation.

[0065] The plurality of optically opaque barriers can be disposed between the plurality of capillaries 105. For example, a first barrier 255 (e.g., first optically opaque barrier) of the plurality of barriers can be disposed between the first capillary 205 and the second capillary 210. The first barrier 255 can prevent light originating from the sample inside the first capillary 205 from reflecting off of the second capillary 210. The first barrier 255 can prevent light originating from the sample inside the second capillary 210 from reflecting off of the first capillary 205.

[0066] A second barrier 260 (e.g., second optically opaque barrier) of the plurality of barriers can be disposed between the second capillary 210 and the third capillary 215. The second barrier 260 can prevent light originating from the sample inside the second capillary 210 from reflecting off of the third capillary 215. The second barrier 260 can prevent light originating from the sample inside the third capillary 215 from reflecting off of the second capillary 210.

[0067] In some embodiments, the first fiber 220 is woven between the first capillary 205 and the first barrier 255. The first fiber 220 can be woven around the first capillary 205 and the first barrier 255. For example, the first fiber 220 can be woven around the first capillary first portion 235 and a portion of the first barrier 255 (e.g., first barrier first portion). The first fiber 220 can be woven around the first capillary 205 and the second barrier 260. For example,Atty. Dkt. No. 20210012-01 (130073-0120) the first fiber 220 can be woven around the first capillary first portion 235 and a portion of the second barrier 260 (e.g., second barrier first portion).

[0068] In some embodiments, the first fiber 220 and the plurality of capillaries 105 can be disposed in a potting component. This can allow the first fiber 220 and the plurality of capillaries 105 to be fixed in a particular configuration. Other ways to fix the first fiber 220 and the plurality of capillaries 105 in a particular configuration can include, for example, vacuum sealing, gluing, molding, thermal staking, or clamping. The one or more fibers and the plurality of capillaries 105 can be disposed in the potting component. The potting component can improve the cooling / heating performance and mitigate air gaps between the plurality of capillaries 105 and the cooling / heating fibers / tubes. The potting component can be a liquid that is poured onto the interwoven array. The potting component can be a photoactivated polymer or a time-activated polymer.

[0069] FIG. 3 is a diagram of the assembly 200. In this embodiment, the assembly 200 includes the first fiber 220 and the second fiber 225. The second fiber 225 can be disposed at a first portion 305 of each of the plurality of capillaries 105. The first fiber 220 is disposed at a second portion 310 of each of the plurality of capillaries 105.

[0070] The assembly 200 can include an optical window 315. The first fiber 220 can be disposed a distance (e.g., separation distance) from the second fiber 225. The distance between the first fiber 220 and the second fiber 225 can define a width 320 of the optical window 315. The optical beam can pass though the optical window 315 and be received by the detector 135. The optical window 315 can include a portion of the plurality of capillaries 105 that does not include one or more fibers woven between and around the plurality of capillaries 105. The optical window 315 can include a portion of the plurality of barriers that does not include one or more fibers woven between and around the plurality of barriers. Optical coupling can be implemented at the optical window 315. For example, optical coupling and / or detectionAtty. Dkt. No. 20210012-01 (130073-0120) (e.g., excitation and emission) can occur at any point between the first end 106 of each of the plurality of capillaries 105 and the second end 107 of each of the plurality of capillaries 105.

[0071] FIG. 4 is a diagram of a cross-section of the assembly 200. In this embodiment, the assembly 200 includes the first fiber 220, the second fiber 225, the plurality of capillaries 105 (e.g., the first capillary 205 and the second capillary 210), and the plurality of barriers (e.g., first barrier 255 and second barrier 260).

[0072] The first fiber 220 can have a width (e.g., first fiber width 405). The first fiber width 405 can be equal to the diameter of the first fiber 220. The first fiber width 405 can be decreased by increasing the tension on the first fiber 220. The first fiber width 405 can be constant or vary along the length of the first fiber 220.

[0073] The second fiber 225 can have a width (e.g., second fiber width 410). The second fiber width 410 can be equal to the diameter of the second fiber 225. The second fiber width 410 can be decreased by increasing the tension on the second fiber 225. The second fiber width 410 can be greater than, less than, or equal to the first fiber width 405. The second fiber width 410 can be constant or vary along the length of the second fiber 225.

[0074] Each capillary of the plurality of capillaries 105 can have a width (e.g., capillary width 415). For example, the first capillary 205 can have the capillary width 415. The second capillary 210 can have the capillary width 415. The width of the first capillary 205 can be greater than, less than, or equal to the width of the second capillary 210.

[0075] Each barrier of the plurality of barriers can have a width (e.g., barrier width 420). For example, the first barrier 255 can have the barrier width 420. The second barrier 265 can have the barrier width 420. The width of the first barrier 255 can be greater than, less than, or equal to the width of the second barrier 265. The barrier width 420 can be greater than, less than, or equal to the capillary width 415. The barrier width 420 can be constant or vary along the length of each barrier of the plurality of barriers.Atty. Dkt. No. 20210012-01 (130073-0120)

[0076] Each capillary of the plurality of capillaries 105 can be separated from a neighboring capillary of the plurality of capillaries 105 by a distance 425. For example, the first capillary 205 and the second capillary 210 can be separated by the distance 425. If there is no barrier (e.g., first barrier 255, second barrier 260) between the first capillary 205 and the second capillary 210, the distance 425 can be equal to or less than the first fiber width 405 or the second fiber width 410. If there is a barrier between the first capillary 205 and the second capillary 210, the distance 425 can be equal to or greater than the barrier width 420.

[0077] Each capillary of the plurality of capillaries can be separated from each barrier of the plurality of barriers by a distance 430. For example, the first capillary 205 and the first barrier 255 can be separated by the distance 430. The first capillary 205 and the second barrier 260 can be separated by the distance 430. The second capillary 210 and the first barrier 255 can be separated by the distance 430. The second capillary 210 and the second barrier 260 can be separated by the distance 430. The distance 430 can be greater than, less than, or equal to the first fiber width 405 or the second fiber width 410.

[0078] FIG. 5 is a diagram of weaving patterns. The weaving patterns show the first fiber 220 and the plurality of capillaries 105. The weaving patterns can include, for example, plain weave (e.g., tabby weave, linen weave, taffeta weave), twill weave, or atlas weave. The first fiber 220 can be woven between and around the plurality of capillaries 105 according to one more of the weaving patterns shown in FIG. 5. Other weaving patterns not shown here can be used as well.

[0079] FIG. 6 is a view of the assembly 200. The assembly 200 can include the plurality of capillaries 105 (e.g., fused-silica capillaries). The plurality of capillaries 105 can be arranged in a braided configuration. The detector 135 can be configured to couple with a portion of the plurality of capillaries between the first end 106 of each of the plurality of capillaries 105 and the second end 107 of each of the plurality of capillaries 105. The second end 107 of each ofAtty. Dkt. No. 20210012-01 (130073-0120) the plurality of capillaries 105 can receive the sample. Braiding can be used to generate a reproducible and / or densely packed capillary array.

[0080] FIG. 7 is a view of the assembly 200. The assembly 200 can include the plurality of capillaries 105 arranged in a braided configuration. The plurality of capillaries 105 can form a tube (e.g., tube structure). The plurality of capillaries 105 can be in a cylindrical configuration. The plurality of capillaries 105 can form a capillary braid. In some embodiments, a rod can be disposed in the tube. For example, the plurality of capillaries 105 can surround the rod. The plurality of capillaries 105 can be woven around the rod. The rod can heat the plurality of capillaries 105. The rod can cool the plurality of capillaries 105. For instance, the rod may comprise a thermoelectric cooler / heater. Alternatively, a cooling or heating liquid is flowing through the rod. The rod may also include heating wires (e.g. resistive heaters). The rod can provide a supporting structure for the plurality of capillaries 105. The plurality of capillaries 105 can be braided around a heated core or cooled core.

[0081] One aspect of the present disclosure is directed to a method for forming an interwoven capillary array. In brief summary, the method can include weaving the first fiber. The method can include positioning the portion of the first capillary parallel to the portion of the second capillary. The method can include positioning the first portion of the first fiber parallel to the second portion of the first fiber.

[0082] The method can include weaving the first fiber. For example, the method can include weaving the first fiber between the first capillary of the plurality of capillaries and the second capillary of the plurality of capillaries. The method can include weaving the first fiber around the portion of the first capillary (e.g., first capillary first portion). The method can include weaving the first fiber around the portion of the second capillary (e.g., second capillary first portion).Atty. Dkt. No. 20210012-01 (130073-0120)

[0083] The method can include positioning the portion of the first capillary parallel to the portion of the second capillary. For example, the method can include positioning the first capillary first portion parallel to the second capillary first portion.

[0084] The method can include positioning the first portion of the first fiber (e.g., first fiber first portion) parallel to the second portion of the first fiber (e.g., first fiber second portion). For example, the method can include positioning the first fiber first portion parallel to the first fiber second portion.

[0085] In some embodiments, the detector is configured to couple with a portion of the plurality of capillaries between the first end of each of the plurality of capillaries and the second end of each of the plurality of capillaries. For example, the method can include coupling the detector with a portion of the plurality of capillaries between the first end of each of the plurality of capillaries and the second end of each of the plurality of capillaries. In some embodiments, the first end of each of the plurality of capillaries can be coupled with the detector. For example, the method can include coupling the first end of each of the plurality of capillaries with the detector. In some embodiments, the second end of each of the plurality of capillaries can receive the sample. For example, the method can include receiving the sample by the second end of each of the plurality of capillaries.

[0086] In some embodiments, the method can include weaving, by a loom (e.g., weaving table), the first fiber and the plurality of capillaries. The plurality of capillaries can be (e.g., constitute) warp. For example, the plurality of capillaries can be held stationary in tension on the loom (e.g., frame). The first fiber can be (e.g., constitute) weft. For example, the first fiber can be drawn through (e.g., inserted over and under) the plurality of capillaries.

[0087] In some embodiments, the method can include weaving the second fiber between the first capillary of the plurality of capillaries and the second capillary of the plurality of capillaries. The method can include weaving the second fiber around a second portion of theAtty. Dkt. No. 20210012-01 (130073-0120) first capillary (e.g., first capillary second portion). The method can include weaving the first fiber around a second portion of the second capillary (e.g., second capillary second portion).

[0088] Any references to implementations or elements or acts of the systems and methods herein referred to in the singular can include implementations including a plurality of these elements, and any references in plural to any implementation or element or act herein can include implementations including only a single element. References in the singular or plural form are not intended to limit the presently disclosed systems or methods, their components, acts, or elements to single or plural configurations. References to any act or element being based on any information, act or element may include implementations where the act or element is based at least in part on any information, act, or element.

[0089] While operations can be depicted in the drawings in a particular order, such operations are not required to be performed in the particular order shown or in sequential order, and all illustrated operations are not required to be performed. Actions described herein can be performed in a different order.

[0090] Any implementation disclosed herein may be combined with any other implementation, and references to “an implementation,” “some implementations,” “an alternate implementation,” “various implementations,” “one implementation” or the like are not necessarily mutually exclusive and are intended to indicate that a particular feature, structure, or characteristic described in connection with the implementation may be included in at least one implementation. Such terms as used herein are not necessarily all referring to the same implementation. Any implementation may be combined with any other implementation, inclusively or exclusively, in any manner consistent with the aspects and implementations disclosed herein.

[0091] References to “or” may be construed as inclusive so that any terms described using “or” may indicate any of a single, more than one, and all of the described terms.Atty. Dkt. No. 20210012-01 (130073-0120) References to at least one of a conjunctive list of terms may be construed as an inclusive OR to indicate any of a single, more than one, and all of the described terms. For example, a reference to “at least one of ‘A’ and ‘B’” can include only ‘A’, only ‘B’, as well as both ‘A’ and ‘B’. Elements other than ‘A’ and ‘B’ can also be included.

[0092] The systems and methods described herein may be embodied in other specific forms without departing from the characteristics thereof. The foregoing implementations are illustrative rather than limiting of the described systems and methods.

[0093] Where technical features in the drawings, detailed description or any claim are followed by reference signs, the reference signs have been included to increase the intelligibility of the drawings, detailed description, and claims. Accordingly, neither the reference signs nor their absence have any limiting effect on the scope of any claim elements.

[0094] The systems and methods described herein may be embodied in other specific forms without departing from the characteristics thereof. The foregoing implementations are illustrative rather than limiting of the described systems and methods. Scope of the systems and methods described herein is thus indicated by the appended claims, rather than the foregoing description, and changes that come within the meaning and range of equivalency of the claims are embraced therein.

Claims

Atty. Dkt. No. 20210012-01 (130073-0120)CLAIMSWhat is claimed is:

1. An assembly, comprising:a plurality of capillaries comprising a first capillary and a second capillary; and a first fiber woven between the first capillary and the second capillary, around a portion of the first capillary, and around a portion of the second capillary,wherein the portion of the first capillary and the portion of the second capillary are parallel,wherein a first portion of the first fiber and a second portion of the first fiber are parallel,wherein a detector is configured to couple with a portion of the plurality of capillaries between a first end of each of the plurality of capillaries and a second end of each of the plurality of capillaries, andwherein the second end of each of the plurality of capillaries is configured to receive a sample.

2. The assembly of claim 1, comprising:a second fiber disposed a first distance from the second end of each of the plurality of capillaries,wherein the first fiber is disposed a second distance from the second end of each of the plurality of capillaries, andwherein the first distance is greater than the second distance.

3. The assembly of claim 1, wherein the first fiber is configured to heat each capillary of the plurality of capillaries.Atty. Dkt. No. 20210012-01 (130073-0120)4. The assembly of claim 1, wherein the first fiber is configured to cool each capillary of the plurality of capillaries.

5. The assembly of claim 1, wherein the first fiber is configured to emit light to each capillary of the plurality of capillaries.

6. The assembly of claim 1, comprising:a plurality of barriers disposed between the plurality of capillaries.

7. The assembly of claim 1, wherein the first fiber comprises a tube configured to circulate a liquid.

8. The assembly of claim 1, comprisinga second fiber disposed at a first portion of each of the plurality of capillaries, wherein the first fiber is disposed at a second portion of each of the plurality of capillaries,wherein the first fiber is disposed a distance from the second fiber, andwherein the distance defines a width of an optical window.

9. The assembly of claim 1, wherein the portion of the first capillary and the portion of the second capillary are perpendicular to the first portion of the first fiber and the second portion of the first fiber.

10. The assembly of claim 1, wherein:Atty. Dkt. No. 20210012-01 (130073-0120) the plurality of capillaries comprises a third capillary,the second capillary disposed between the first capillary and the third capillary, and the first fiber woven between the second capillary and the third capillary and around a portion of the third capillary.

11. The assembly of claim 1, wherein the first fiber comprises at least one of metal, glass, polymer, or thread.

12. The assembly of claim 1, wherein a distance between the first capillary and the second capillary is greater than or equal to a diameter of the first fiber.

13. The assembly of claim 1, wherein the first fiber and the plurality of capillaries are disposed in a potting component.

14. An assembly, comprising:a plurality of capillaries arranged in a braided configuration,wherein a detector is configured to couple with a portion of the plurality of capillaries between a first end of each of the plurality of capillaries and a second end of each of the plurality of capillaries, andwherein the second end of each of the plurality of capillaries is configured to receive a sample.

15. The assembly of claim 14, wherein the plurality of capillaries form a tube.

16. The assembly of claim 15, comprising:Atty. Dkt. No. 20210012-01 (130073-0120) a rod disposed in the tube and configured to heat the plurality of capillaries.

17. The assembly of claim 15, comprising:a rod disposed in the tube and configured to cool the plurality of capillaries.

18. A method, comprising:weaving a first fiber between a first capillary of a plurality of capillaries and a second capillary of the plurality of capillaries, around a portion of the first capillary, and around a portion of the second capillary;positioning the portion of the first capillary parallel to the portion of the second capillary; andpositioning a first portion of the first fiber parallel to a second portion of the first fiber,wherein a detector is configured to couple with a portion of the plurality of capillaries between a first end of each of the plurality of capillaries and a second end of each of the plurality of capillaries, andwherein a second end of each of the plurality of capillaries is configured to receive a sample.

19. The method of claim 18, comprising:weaving, by a loom, the first fiber and the plurality of capillaries,wherein the first fiber comprises weft and the plurality of capillaries comprises warp.

20. The method of claim 18, comprising:Atty. Dkt. No. 20210012-01 (130073-0120) weaving a second fiber between the first capillary and the second capillary, around a second portion of the first capillary, and around a second portion of the second capillary.

Images