Methods for identifying SMV resistance in genetically modified soybeans using gene editing and interference

Abstract

The present invention provides a method for identifying the resistance to Soybean Mosaic Virus (SMV) in genetically improved soybeans obtained by techniques such as gene editing, transgenesis, and RNAi interference. The method comprises the following steps: (1) providing the soybean variety to be evaluated; (2) separately preparing a first solution containing carborundum and a second solution containing Soybean Mosaic Virus, and mixing the first solution and the second solution in a certain ratio to obtain a working solution; (3) spraying the working solution obtained in step (2) onto the leaves of the soybean variety to be evaluated by using a spray gun inoculation method, so that the Soybean Mosaic Virus invades the leaves; (4) investigating the disease occurrence after a period of inoculation in step (3), and identifying the SMV resistance level of the soybean variety to be evaluated according to the disease performance.