Insect pathogenic nematode symbiotic bacterium fermentation supernatant preparation, preparation method and application thereof

Abstract

The present invention belongs to the field of biotechnology, and specifically relates to an insect pathogenic nematode symbiotic bacterium fermentation supernatant preparation, a preparation method and an application thereof. The preparation includes Serratia marcescens fermentation supernatant and auxiliary agents. The preparation method includes S1: picking single colonies on an NBTA differential medium; S2: picking single colonies on an NA medium; S3: culturing in an LB liquid medium; S4: performing shake flask fermentation culture; S5: centrifuging to obtain a supernatant; S6: mixing the supernatant with auxiliary agents uniformly to obtain the insect pathogenic nematode symbiotic bacterium fermentation supernatant preparation. The present invention also provides an application of the insect pathogenic nematode symbiotic bacterium fermentation supernatant preparation in preventing and controlling Chinese chives gray mold and tomato gray mold. The insect pathogenic nematode symbiotic bacterium fermentation supernatant preparation of the present invention has a significant control effect on Chinese chives gray mold and tomato gray mold.