Arms-PCR primer set for selecting disease-resistant cattle based on genetic polymorphism and use thereof

Abstract

The present invention provides an ARMS-PCR primer set for selecting disease-resistant cattle based on genetic polymorphism and use thereof, and belongs to the field of gene-assisted breeding technology. The ARMS-PCR primer set for selecting disease-resistant cattle based on genetic polymorphism provided by the present invention includes an upstream outer primer 12-PF3, an upstream inner primer 12-IF2, a downstream inner primer 12-Rin, and a downstream outer primer 12-LR1. The ARMS-PCR primer set according to the present invention can specifically detect the insertion and deletion homozygotes and the heterozygous genotypes thereof at the 12 bp insertion / deletion polymorphic site in the intronic region of the bovine prion protein gene, and gene-assisted breeding of cattle and detection of cattle-related products can be performed based on the genotypes, and a basis for evaluating susceptibility to bovine spongiform encephalopathy may be provided.

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