Tissue culture method of rhodiola crenulata

Abstract

The invention provides a tissue culture method of Rhodiola crenulata, belonging to the technical field of plant tissue culture. According to the invention, a plurality of explants (callus, leaves and stem segments) of Rhodiola crenulata tissue culture seedlings are utilized to induce adventitious roots, and then propagation culture is carried out, so that the composition of the medium is limited, so that the induction rate of adventitious roots is between 58.3% and 72.6%, and the browning rate is low in the process of tissue culture adventitious roots of Rhodiola crenulata, which lays a foundation for industrialized production of important active components of Rhodiola crenulata by using adventitious roots in the future, and can solve the problem of raw materials of Rhodiola crenulata.