A high-efficiency snadf bio-denitrification device and method based on endogenous biomass fermentation

Abstract

The present invention relates to the field of biological nitrogen removal technology, specifically to a SNADF (Short-range Nitrification, Anammox, Denitrification, and Fermentation) high-efficiency bio-denitrification device and method based on endogenous biomass fermentation. The invention adopts a starvation strategy, in which the carbon source supply is stopped to enrich OLB8 bacteria and Pedosphaeraceae bacteria that use endogenous organic matter (such as extracellular polymeric substances (EPS) and cell lysate products) as metabolic substrates. The fermentation of endogenous biomass such as EPS provides a carbon source for the denitrification system, thereby constructing a carbon self-sufficient SNADF process that integrates short-range nitrification, anaerobic ammonium oxidation (Anammox), denitrification, and fermentation. The specific technical scheme is as follows: In the reactor, wastewater and livestock and poultry waste are added. After starting the short-range nitrification process, before inoculating Anammox granular sludge, the effluent ammonium nitrogen and nitrite concentrations are controlled at 80-150 mg / L with a ratio close to 1:1. Dissolved oxygen (DO) is maintained at 0.3-0.5 mg / L, and pH is controlled at 7.5-8.0. The total nitrogen removal rate of the reactor exceeds 85 percentage, indicating that the short-range nitrification / anaerobic ammonium oxidation process has successfully. The starvation strategy is then repeated 3-5 times. During the starvation implementation, effluent ammonium nitrogen shall not exceed 50 mg / L, nitrite shall not exceed 20 mg / L, and the total nitrogen removal rate of the reactor shall be higher than 93 percentage, indicating that the carbon self-sufficient denitrification and fermentation process has successfully started. By using endogenous biomass such as EPS as a fermentation substrate, the SNADF process is realized in the reactor, enabling high-level nitrogen removal, sludge reduction, and low-energy operation simultaneously.