In-situ remediation method for uranium contamination through bio-induced pyrite transformation

Abstract

The invention discloses an in-situ remediation method for uranium contamination. The method immobilizes uranium contaminants by exploiting interactions between microorganisms and pyrite, thereby driving pyrite hydrolysis, oxidation, atomic rearrangement, and biomineralization. Through these processes, uranium contaminants are fixed and encapsulated within the crystal lattice of reconstructed iron oxide products, achieving effective remediation of uranium-contaminated areas. The microorganisms include one or more of Brucella sp., Geobacter sp., and Clostridium sp. Utilizing indigenous microorganisms in the contaminated area, natural humic substances are used as electron donors to stimulate in-situ microbial activity. A humic substance solution is injected into the uranium-contaminated area by cyclic pumping, with a concentration of 0.05-5 g / L and a dosage of 5-500 g / day. The present method fully considers and rationally utilizes in-situ minerals, microorganisms, and geochemical resources. By relying on interactions between naturally occurring minerals and microorganisms, the method reduces engineering costs and achieves high remediation efficiency.