A space-time calibration system and method for whole-plate high-throughput combined photoelectric detection

Abstract

The present invention relates to the technical field of photoelectric measurement and calibration, in particular to a space-time calibration system and method for whole-plate high-throughput combined photoelectric detection. The system comprises: a time alignment module, which is used for accurately determining and aligning exposure windows; a spatial alignment module, which is used for multi-level dynamic focal plane compensation; an adaptive light source shaping system, which is used for realizing pixel-level uniformity of excitation light; and an electrode artifact positioning and correction system, which is used for eliminating optical artifacts of electrodes. The present invention provides a space-time calibration system and method for whole-plate high-throughput combined photoelectric detection, which solves or at least alleviates the problems such as time synchronization, focal plane drift, illumination uniformity and electrode artifacts existing in whole-plate high-throughput photoelectric detection in the prior art, realizes comprehensive focal plane compensation and signal fidelity, effectively addresses the challenges of space-time accuracy and uniformity, and provides an efficient and accurate technical foundation for biomedical detection.

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