Vector engineering strategies to enhance volumetric productivity and decrease impurity formation across a diverse set of modalities
Patent Information
- Application Number
- ZA202607250
- Authority / Receiving Office
- ZA · ZA
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-01-19
- Filing Date
- 2026-07-15
- Publication Date
- 2026-07-29
AI Technical Summary
The high cost and complexity of manufacturing biologies, particularly antibodies with multiple chains, hinder their widespread adoption as front-line therapies due to the need for optimized cell lines, large-scale culturing, and purification processes, necessitating improved expression systems for higher titers and reduced impurities.
Employing expression cassettes with specific promoter arrangements and polyA signal sequences, such as CMV/GAPDH and SV40, to drive balanced expression of antibody chains in mammalian host cells, including CHO cells, to enhance titers and minimize impurities.
The proposed expression cassettes and vectors increase antibody production titers while maintaining product quality, reducing aggregation and clipping, and enabling more cost-effective and consistent biopharmaceutical production.