37 results about "Amino acid sequence homology" patented technology

Differentially expressed Leishmania genes and proteins are described. One differentially expressed gene (A2) is expressed at significantly elevated levels (more than about 10 fold higher) in the amastigote stage of the life cycle when the Leishmania organism is present in macrophages than in the free promastigote stage. The A2 gene encodes a 22 kD protein (A2 protein) that is recognized by kala-azar convalescent serum and has amino acid sequence homology with an S-antigen of Plasmodium falcilparum Vietnamese isolate VI. Differentially expressed Leishmania genes and proteins have utility as vaccines, diagnostic reagents, as tools for the generation of immunological reagents and the generation of attenuated variants of Leishmania.

The invention relates to a method for using rice auxin to induce protein genes to be cloned, which is characterized in that the implementation steps comprise: (1) the preparation of a rice transformation receptor; (2) the genetic transformation of the rice; (3) the screening of kanamycin-resistant callus tissue and the regeneration of plants; (4) the screening of the mutant of a T-DNA inserted progenies; (5) Tail-PCR; (6) the comparison and analysis of sequences on the Internet. In the invention, a rice mutant with a short plant height is obtained when carrying out rice functional genome research by using T-DNA label method; the lateral neighboring sequences of the mutant are researched by using TAIL-PCR technology; meanwhile, the position where the mutant T-DNA inserts the rice genome is arranged on the No. 4 chromosome of the rice by the comparison on the databases of NCBI and TIGR on the Internet; moreover, the rice BAC clone (OSJNBa0084K01) of the position is found out. The T-DNA is inserted between the two genes of the clone by analyzing the clone. Known functional genes with a very high homology with BAC cloning code amino acid sequence are forecasted by the sequence comparison on the Internet.

The invention provides a novel plant strong salt tolerant gene AtNHXS1 acquired through DNA reshuffling technology, and the ionic transport activity of Na+/H+ antiporter protein coded by the gene is stronger than that of wild Na+/H+ antiporter protein ATNHX1. A nucleotide sequence of the gene as shown in SEQ ID NO:1 also comprises genes of the nucleotide sequence of sequence 1 in a sequence table with the homology between 70 and 100 percent, or a nucleotide sequence of an amino acid sequence of sequence 2 in a coded sequence table. The new Na+/H+ antiporter has a protein of the amino acid sequence of sequence 2 in the sequence table, protein of the amino acid sequence of the sequence 2 with the homology between 70 and 100 percent, or protein which replaces, deletes or adds one or a plurality of amino acids in the amino acid sequence of the sequence 2 with the same homology. The invention also provides the construction of recombinant vector, the transgenic plant and other methods so as to apply the gene and the protein, thereby cultivating a novel variety of transgenic plant with strong capacity of salt tolerance or other improved biologic characters.

The invention belongs to the technical field of biomedicine, specifically relates to a use of an interleukin-1receptor antagonist (IL-1Ra), and pharmaceutical compositions of the IL-1Ra. The invention discloses a use of protein selected from the following (a) or (b) in preparing drugs for treating or preventing a cardiac toxic reaction due to chemotherapeutics: (a) amino acid sequence of the protein is represented by SEQ ID NO.1; (b) the protein shares 70% amino acid sequence identity with the protein provided by the (a), and has activities for treating or preventing the cardiac toxic reaction due to the chemotherapeutics. According to the protein provided by the present invention, the cardiac toxic reaction due to the chemotherapeutics can be effectively removed or reduced, and a new selectivity may be provided for the future clinical treatment of tumor.

The invention discloses a novel fish odinagogue, which is a polypeptide having an amino acid sequence of Tyr-Asn-Lys-Asn-Ser-Phe-Gly-Lys-Arg-Tyr or a polypeptide having an amino acid sequence which has an over 60-percent homology with the amino acid sequence of Tyr-Asn-Lys-Asn-Ser-Phe-Gly-Lys-Arg-Tyr. When use, the polypeptide is dissolved in normal saline for fish according to a ratio of 1 milligram peptide per milliliter of normal saline; the injection amount is 0.5 to 1mu g per gram of fish; and the solution is injected into the fish at a position near the base part of the pectoral fin or the muscle of the dorsal fin. The novel fish odinagogue can induce the ovulation of goldfish, crucian and loach, and is novel fish odinagogue with an important production prospect.

The invention relates to the technical field of rabies application, in particular to a rabies virus recombinant antigen and a preparation method and application thereof. The rabies virus recombinant antigen comprises one of the following parts (a) to (c): (a) amino acid sequence fragments N, G and P are sequentially included in the direction from an end N to an end C; (b) the rabies virus recombinant antigen is obtained by substituting, deleting or adding at least one or more amino acids to the amino acid sequence fragments N, G and/or P in (a), and an immune reaction of mammals to the rabiesvirus recombinant antigen can be induced; or (c) an amino acid sequence having homology with the amino acid sequence of the rabies virus recombinant antigen in (a) is obtained, and an immune reactionof the mammals to the rabies virus recombinant antigen can be induced. The rabies virus recombinant antigen has an antigen epitope with good antigenicity and small non-specificity. Compared with a whole-virus antigen, the rabies virus recombinant antigen has the advantages of stability, safety, low culture cost and high efficiency.

The invention discloses a new lycine aldehyde dehydrogenase gene BnBADH-1 in cabbage type rape. The nucleotide sequence is as shown in SEQ ID NO:1; and the coded amino acid sequence is as shown in SEQ ID NO:2. A new BADH gene sequence is obtained by cloning in cabbage type rape and is named as BnBADH-1. Compared with Arabidopsis thaliana BADH 1 (ALDH10A8), the BnBADH-1 has a homology of 90.24% in nucleic acid sequence and a homology of 93.41% in amino acid sequence. Compared with another rape BADH (AY351634) existing in GeneBank, the BnBADH-1 has a homology of 75.79% in nucleic acid sequence and a homology of 77.93% in amino acid sequence. Thus, the BnBADH-1 is a new gene different from the AY351634.

Hog monoamine oxidase B protein coding sequence is characterized by having nucleotide sequence from 129-1691 bit in SEQ IDNO.1 and monoamine oxidase B protein coding sequence homology 92.73% with human, rat, ox and dog, having amino acid sequence polypeptide in SEQ ID No:1, or its conserved variant polypeptide, or its active fragment, or its active derivative, having monoamine oxidase B amino acid sequence homology 93.81% with human, rat, ox and dog, cloning according MAO-B gene sequence, sequencing, obtaining hog MOA-B cDNA sequence, and homologous comparing for different species CDS sequence of MAO-B gene. It can be used for relation of MAO-gene and hog stress sensitivity and human related stress diseases.

The invention discloses construction, a production method and application of of a high-performance starch debranching enzyme chimera strain. A high-performance starch debranching enzyme chimera is obtained by effective chimeric combination of two parental pullulanase amino acid sequences, and the homology of the high-performance starch debranching enzyme chimera and the parental pullulanase amino acid sequences is not less than 95%; and the high-performance starch debranching enzyme chimera is obtained by carrying out chimeric combination on amino acid residues from site 1 to site 476 at the tail end of parental pullulanase of bacillus acidopullulyticus and amino acid residues from site 465 to site 976 at the tail end of parental pullulanase of bacillus deramificans. The chimeric pullulanase has a relatively high saccharification rate, also has higher heat resistance and thermal stability than the parental pullulanase, and is suitable for a saccharification reaction and starch liquefaction.

The invention discloses a recombinant protein for resisting porcine pseudorabies virus reproduction, and application of the recombinant protection to synthesis of a medicine for resisting porcine pseudorabies virus reproduction. The recombinant protein for resisting porcine pseudorabies virus reproduction is a protein with an amino acid sequence consisting of an amino acid sequence shown as SEQ ID No.2, a protein with an amino acid sequence which has homology of 95-100 percent with the amino acid sequence limited by the sequence SEQ ID No.2, and is used for coding proteins with same functions, or a protein which is derived from the amino acid sequence shown as SEQ ID No.2 through addition, deletion or substitution of one or more amino acids and has equal activity. The recombinant protein provided by the invention is used in the medicine for resisting the porcine pseudorabies virus reproduction; experiments prove that the recombinant protein has a remarkable inhibition effect.

This invention relates to a novel mycobacterial protein named DES, which appears to share significant amino acid sequence homology with soluble stearoyl-ACP desaturases. The results of allelic exchange experiments, indicate that the des gene may be essential to the survival of mycobacteria. These results coupled with the surface localization, the unique structure of DES, and the fact this antigen is expressed in vivo, and DES protein induces a humoral response in human patients, indicate that the DES protein provides a new target for the design of anti-mycobacterial drugs. This invention provides methods of screening molecules that can inhibit the DES enzyme activity of purified DES protein, in order to identify antibiotic molecules that are capable of inhibiting the growth or survival of mycobacteria. These methods may be practiced by using recombinant DES protein obtained from a recombinant mycobacterium host cell that was transformed with a vector containing the des gene, whose expression is controlled by regulatory or promoter sequences that function in mycobacteria. Another aspect of this invention relates to the molecules that have been identified according to the screening methods as having antibiotic activity against mycobacteria.

The invention belongs to the field of immunology and particularly relates to Plasmodium berghei gametophyte recombinant protein (rPbG37), a preparation method thereof and application of the Plasmodium berghei gametophyte recombinant protein (rPbG37) in malaria transmission blocking. The amino acid sequence of the Plasmodium berghei gametophyte recombinant protein (rPbG37) is as shown in SEQ ID No. 1; or the amino acid sequence of the Plasmodium berghei gametophyte recombinant protein (rPbG37) is an amino acid sequence whose homology with an amino acid sequence defined by the sequence as shown in SEQ ID No. 1 is 95-100% and encoding identical functional protein; or the amino acid sequence of the Plasmodium berghei gametophyte recombinant protein (rPbG37) is an amino acid sequence obtained by increasing, deleting or replacing one or more amino acid of the amino acid sequence as shown in SEQ ID No. 1 and provided with identical-activity derivative protein. The recombinant protein is novel recombinant protein with a transmission blocking effect and can further increase a malaria transmission blocking effect.

The present invention provides a process for producing a useful substance by use of a microorganism that lacks in their chromosomal DNA all or part of a gene encoding a protein having the amino acid sequence shown in SEQ ID NO: 1 or a gene encoding a protein having 80% or more, preferably 90% or more, more preferably 95% or more, even more preferably 97% or more, still more preferably 98% or more, and yet more preferably 99% or more homology with the amino acid sequence shown in SEQ ID NO: 1.

The invention belongs to the technical field of agaricus bisporus genetic engineering, and particularly relates to agaricus bisporus heme dioxygenase and application thereof. A known amino acid sequence of fungal heme dioxygenase is utilized, comparing with an agaricus bisporus genome sequence,and according to the agaricus bisporus heme dioxygenase, the heme dioxygenase of agaricus bisporus is obtained, the heme dioxygenase has a CYP domain. The amino acid sequence homology with the PpoC of aspergillus nidulans is 33%, the similarity is 51%, but the CYP domain of the heme dioxygenase is the same as that of the PpoC, a complete P450 characteristic consensus sequence heme conservative binding region is not contained, it is speculated that the CYP domain of the heme dioxygenase possibly has no function, and the heme dioxygenase is named AbDOX-(CYP). The cloning of the gene and the conversion expression of escherichia coli further show that the heme dioxygenase can catalyze linoleic acid to synthesize 1-octene-3-ol. On the basis of the evidence, a good technical foundation can be laid for further industrial production improvement of the 1-octene-3-ol.