Bacteria TRIZ Cases

Engineered Pseudomonas putida secretes hydrophobic products via outer membrane vesicles, avoiding cell lysis and simplifying purification.

Selective enzymatic modification of steviol glycosides yields sucrose-like taste profiles while eliminating the need for high-purity starting materials.

Targeted amino acid substitutions at positions 35, 39, and 78 enhance enzyme activity to resolve suboptimal biosynthesis pathway efficiency.

Deleting cre-like regulatory elements from Bacillus promoters eliminates glucose-induced catabolite repression, sustaining high enzyme productivity.

Universal peptide tags enable rapid polypeptide quantification via mass spectrometry.

Deleting fhuC, fhuD, and fhuB genes eliminates the FhuCDB protein complex to conserve intracellular ATP for enhanced L-amino acid biosynthesis.

Engineered Escherichia coli strains with enhanced malate dehydrogenase and thiolase activities for isopropyl alcohol production.

Inoculating Oceanobacillus picturae into brine increases volatile substance variety while decreasing pungent compounds to enhance pickled vegetable flavor.

Engineered dehydrogenases degrade norleucine within host cells to prevent non-standard amino acid incorporation into recombinant proteins.

Recombinant Escherichia coli expresses specific enzymes to synthesize asiaticoside, overcoming low plant yields and enabling industrial-scale production.

Deleting FruA and FruK genes eliminates feedback inhibition, resolving the trade-off between uptake ability and intracellular glucose accumulation.

Engineered E. coli produces microcin H47 via signal-induced promoters, resolving antibiotic resistance and microbiota disruption during infection.

Bacterial peptides trigger host monocytes to produce IL-10, avoiding side effects from high-dose recombinant administration.

Novel Euglena desaturase enzymes resolve low conversion efficiency by enabling high-specificity production of omega-3 and omega-6 fatty acids.

Targeted gene deletions in recombinant E. coli reduce endogenous alcohol dehydrogenase activity, resolving the bottleneck of aldehyde conversion to alcohols.

Engineered hexuronate C4-epimerase variant converts D-fructose to D-tagatose via specific amino acid mutations.

Engineered mucoid strains maintain stable phenotypes to overcome seasonal seaweed harvest limits and low yields.

Modified bacteriocin tail fibers alter binding affinity to overcome antibiotic resistance in Pseudomonas aeruginosa infections.

Algal acyl-ACP thioesterase resolves the trade-off between lipid productivity and chain length control by leveraging distinct enzyme kinetics.

Recombinant endolysins target lactobacilli cell walls to prevent ethanol yield losses from bacterial contamination.

Overexpressing succinate dehydrogenase genes in microorganisms to boost methionine production yields.

Segmented O-antigen epitopes create an evolutionary trap that breaks the transmission chain of Proteobacteria enteropathogens.

Engineered microbial cells produce fatty aldehydes via fermentation, replacing petroleum extraction and reducing environmental damage.

GOS pre-conditioning improves L. reuteri persistence in the gut, reducing administration frequency while maintaining probiotic efficacy.

Photosynthetic membrane vesicles regenerate ATP via light irradiation to sustain continuous enzymatic synthesis of 5-aminolevulinic acid.

Fermentation of Streptococcus equi subsp. zooepidemicus followed by ultrafiltration produces sodium hyaluronate with controlled molecular weight.

Recombinant microorganisms express lipoxygenase and decarboxylase to catalyze whole-cell biotransformation of unsaturated fatty acids.

Prepartum vaccination with bacterial antigens establishes protective immunity against postpartum uterine infections without antibiotic use.

Agathobaculum butyriciproducens SR79 strain mediates therapeutic effects through the gut-brain axis, inhibiting neuroinflammation without hepatic toxicity.

Site-directed mutagenesis of Bet v 2 allergen creates hypoallergenic variants that lower IgE reactivity to minimize side effects during immunotherapy.

Engineered microbial cells produce branched chain fatty acid derivatives through optimized metabolic pathways.

Fluidized bed agglomeration produces stable nutrient particles that dissolve rapidly without requiring sterilization.

Inactivated Streptococcus thermophilus ST7 maintains storage stability while increasing IL-12p40 expression and Firmicutes/Bacteroidetes ratio.

Lowering microbial culture temperature sustains viability during substrate shortages.

High-performance Lactobacillus strains decompose oxalic acid, addressing insufficient degradation rates in conventional bacteria.

Replacing thioglycolic acid with ascorbic acid maintains detection sensitivity for four months, eliminating specialized equipment needs.

Engineered bacterial minicells display Fc-binding fusion proteins to capture targeting molecules, reducing toxicity to healthy tissues.

Inactivating nanT and nanA while overexpressing neuC, neuB, and glmS in E. coli boosts sialic acid yield to 15 g/liter.

Lactobacillus gasseri LM1065 reduces NO and IL-6 expression while increasing the prostaglandin E1/prostaglandin E2 ratio to relieve premenstrual syndrome.

A coryneform bacterium transformant produces phenol via introduced chorismate-pyruvate lyase and 4-hydroxybenzoate decarboxylase enzymes.

Fusion protein tags resolve inclusion body formation in E. coli, enabling high-yield 2′-fucosyllactose fermentation.

Engineered Lactobacillus plantarum secretes Amuc-1100 to resolve insufficient immune modulation while maintaining metabolic homeostasis.

Biotin supplementation suppresses canthaxanthin accumulation during bacterial fermentation, resolving safety limits while maintaining productivity.

Lactobacillus supplements modulate poultry gut microbiomes to improve feed conversion ratios.

A cloning vector uses a toxic gene with essential and inessential regions to enable positive selection of recombinant plasmids.

Replacing pure glucose with hydrolyzed agro-waste carbon sources reduces production costs while maintaining high L-arginine yields.

Novel probiotic strains metabolize nonfluorescent advanced glycation end products, replacing toxic synthetic inhibitors with safe biological agents.

Sulfate-modified polyvinyl alcohol gel beads encapsulate anaerobic bacteria, relieving organic acid inhibition and maintaining reactor stability.

Endogenous microbial consortium ferments vitivinicultural biomass to produce hydrogen at 37°C without external inoculum.

Deleting fadE blocks beta-oxidation while overexpressing BTE and MAACR enzymes boosts fatty alcohol titer to 1.65 g/L from renewable sugars.