Bacteria TRIZ Cases

Intestine-on-chip co-cultures epithelial and endothelial cells with probiotic bacteria to simulate intestinal motility.

Overexpressing the argD2 gene in Corynebacterium glutamicum enhances L-arginine flux while bypassing natural feedback-inhibition constraints.

Overexpressing acyl carrier protein and synthetase in cyanobacteria boosts lipid yield for biofuel feedstocks.

Pseudomonas sp. ECO-1 produces a bifunctional enzyme preparation that breaks down organic pollutants.

Solid copper substrates supply ions for nanoflower growth, eliminating added copper salts while embedding bacteria and enzymes.

Pseudomonas aeruginosa CSMY-1 biofilm degrades industrial pollutants including dyes, lignin, and arsenic through metabolic processes.

Engineered bacteria expand product diversity by utilizing multiple CoA primers in a reverse beta-oxidation pathway, overcoming limited synthesis capabilities.

Genetically modified Corynebacterium glutamicum improves putrescine and cadaverine yields by enhancing membrane transport proteins.

A Lactobacillus delbrueckii subsp lactis strain directly acidifies cereal suspensions to produce lactic acid without prior microbial adaptation.

Probiotics competitively absorb sialic acid from milk glycans, preventing Enterobacteriaceae colonization and diarrhea.

Optimized temperature and pH conditions enable the immobilized bacterium to achieve 90% degradation efficiency, overcoming low rates of existing strains.

Treating Bacillus natto supernatant with calcium chloride reduces vitamin K2 levels below 1 μg/g, avoiding expensive chitosan and organic solvent residues.

Modulating gut microbiota with tomatidine and probiotics enhances bone strength while reducing osteoporosis side effects.

Cysteine residue replacements form disulfide bonds, preventing phycobiliprotein denaturation during high-temperature manufacturing.

A biochemical viscosity reducer combines lipopeptides and enzymes to lower heavy oil viscosity.

PDC-1 strain degrades polycyclic aromatic hydrocarbons while tolerating heavy metal toxicity that inhibits conventional bacteria.

Cationic antimicrobial peptides reduce the lag phase of slow-growing Mycobacteria, enabling timely diagnosis.

A nitrate-reducing bacteria composition inhibits sulfate-reducing bacteria growth through metabolic competition.

Site-directed mutations at positions 275 and 469 boost conversion activity, resolving low productivity in D-tagatose manufacturing.

Deleting genes in the oxidative pathway suppresses 2,3-butanediol formation and simplifies purification of 1,3-propanediol.

Cell-free protein synthesis platform using Clostridium autoethanogenum extracts enables high-throughput prototyping without anaerobic culture constraints.

Recombinant beta-1,3-galactanase polypeptides hydrolyze specific carbohydrates to enhance solubilization in coffee extraction processes.

Mutating glpR and glpK genes removes feedback inhibition, increasing succinic acid titers from glycerol feedstock.

Microbiota-derived postbiotics replace costly, ethically problematic fetal bovine serum with sustainable alternatives for cultured meat production.

Recombinant CRAMP-expressing probiotic corrects dysbiosis and halts diabetogenic responses by restoring colonic homeostasis during early immune development.

Engineered acetogenic bacteria convert carbon monoxide into 3-hydroxypropionate, resolving low yield and poor gas utilization in industrial fermentation.

Non-ionic detergent buffers lyse blood cells to isolate viable microorganisms, eliminating sub-culturing delays that compromise diagnostic speed.

A Bifidobacterium pseudocatenulatum strain expresses endogenous xylanase genes to degrade complex dietary xylans into usable carbon sources.

Lyophilized biofilms on porous glass substrates enable reliable microbial deployment while eliminating liquid culture handling risks.

Lactic bacteria and fungal proteases break down gluten proteins into non-toxic peptides.

A bacterial genetic system uses a toxin-antidote mechanism to drive high-efficiency over-expression and secretion of recombinant proteins.

Reducing boron, manganese, molybdenum, and copper in the culture medium lowers operational costs without sacrificing specific space-time yield.

Recombining polyketide synthase modules overcomes substrate specificity limits, enabling production of diverse 3-hydroxycarboxylic acids and ketones.

Insertion sequence elements resolve low multicopy insertion efficiency by enabling targeted gene integration into Deinococcus genomes.

Mutated Acinetobacter baumannii cells trigger innate immunity, reducing bacterial burdens in multi-drug resistant infections.

Transformed host cells produce high-titer branched-chain fatty acids via engineered enzymatic pathways.

Aerobic Rhizobium oxidoreductase overcomes anaerobic microbial limits to efficiently produce ginsenoside aglycones.

Bacterial biosensors utilize UrpRS genetic circuits to detect and neutralize bioavailable uranium, eliminating complex external detection equipment.

A genetically modified microorganism uses a biosensor to trigger antitoxin expression, sustaining cell growth and viability.

Functional metagenomics accesses uncultured microbial diversity for novel biosurfactant discovery, overcoming culture-based identification limits.

AAHKAU medium boosts Haematococcus sp. KAU-01 growth to fix combustion gases, overcoming slow strain rates.

Expression of delta6 and delta15 desaturases in transgenic corn increases stearidonic acid levels, reducing reliance on fish-derived omega-3 sources.

S. salivarius DPC6487 produces nisin G to inhibit Fusobacterium without disrupting gut microbiota balance.

Deleting the ilvBN operon leader peptide removes feedback inhibition, allowing high-yield L-valine production without shared enzyme constraints.

Silk fibroin coating resolves manufacturing cost trade-offs by improving acid resistance, bile tolerance, and viability during freeze-drying.

Uridine diphosphate glycosyltransferase B converts rebaudioside A into high-purity sweeteners.

A bacterial strain producing high nitrile hydratase activity for acrylamide synthesis.

A new culture medium uses fatty acid-free bovine serum albumin to enhance mycobacterial growth.

Genetically engineered Pseudomonas strains metabolize carbon in pyrolysis waste streams using overexpressed chaperone proteins.