Fermentation TRIZ Cases

Acid heat treatment stabilizes PHA fermentation broth, preventing metabolic degradation and enabling delayed, simpler recovery at high cell concentrations.

A two-step heterotrophic-autotrophic bacterial process boosts PHA copolymer production rate and supports easier extraction at industrial scale.

Continuous flow-channel mixing of phospholipase D with organic and aqueous reactants shortens phospholipid reaction time and improves production efficiency.

Enzymatic acyloin condensation of formyl-CoA with aldehydes or ketones expands one-carbon feedstocks into substituted 2-hydroxyacyl-CoAs.

Microbial CYP450 hydroxylation replaces plant extraction and chemical synthesis to produce gamma-lactones with higher yield, lower cost, and less impact.

Engineered endoglycoceramidase mutants enable aqueous, donor-efficient glycosphingolipid synthesis with strong stereo- and regiochemical control.

Negatively charged solid media suppress dsRNA during in vitro transcription, preserving mRNA yield, stability, and transfection efficiency.

Using solid MgCl2·4H2O enables lower-temperature thermohydrolysis to produce MgO and HCl faster while avoiding reactor blocking.

Controlled water, methanol, and copper in biomass-derived inhibitors keep propylene polymerization stable and suppress lump polymers.

Keeping reducing sugar at 5 g/L or less during cell culture lowers recombinant protein glycation while preserving production and homogeneity.