Fusion With DNA-Binding Domain TRIZ Cases
- Recombinant Nucleases with TALE Binding for Precise HDR Editing
TALE-linked recombinant nucleases target DNA breaks while steering repair from NHEJ to HDR for more precise and efficient genome editing.
- Cas9 Cytidine Base Editor Fusion for Precise Single-Nucleotide Editing
A Cas9-deaminase-UGI fusion enables precise single-nucleotide editing while avoiding stochastic repair, reducing indels and unwanted mutations.
- Engineered Cas9 PAM Specificity for Broader Genome Targeting
Mutated Cas9 PAM-interacting residues expand targetable genomic sites while preserving editing precision and lowering off-target activity.
- HBV Epigenetic Editing for cccDNA Silencing Without DNA Cleavage
Targeted epigenetic editing silences HBV cccDNA and integrated DNA to suppress replication without DNA cleavage or repair activation.
- Epigenetic T Cell Editing for ACT Expansion and Persistence
Targeted epigenetic editing in T cells tunes gene expression to improve ACT expansion, effector function, and persistence.
- HBV Epigenetic Editing of Regulatory Regions Without DNA Breaks
Targeted epigenetic marks repress HBV regulatory regions in cccDNA and integrated DNA, sustaining viral silencing without DNA breaks.
- Intein-Assembled TALENs for Smaller Plasmids and Flexible Targeting
Inteins self-splice separate TALE and nuclease parts to shrink plasmids while preserving target specificity in genome editing.
- Modified ER-LBD Caspase-9 Kill Switch for Sustained Transgene Control
Modified ER-LBD fused to caspase-9 enables sustained transgene expression and ligand-triggered apoptosis to improve cell therapy safety.
- Recombinant Nucleases for Precise HDR-Guided Genome Editing
Donor-template-guided recombinant nucleases shift DNA repair toward HDR, improving genome editing precision without losing cleavage efficiency.
- AAV Fusion Transactivators for SCN1A Upregulation Under Packaging Limits
Fusion DNA-binding transactivators let AAV vectors boost SCN1A expression despite limited packaging capacity for gene augmentation.
- DNA Polymerase Genome Editing for Precise Sequence Replacement
A nuclease, DNA polymerase, and ssDNA template enable more precise genome sequence replacement with higher editing efficiency and fewer mutations.
- Engineered Transcription Factors for Selective SCN1A Upregulation
Engineered transcription factors and microRNA sites enable selective SCN1A upregulation in parvalbumin neurons while limiting immune response and off-target effects.
- Modular Chimeric Antigen Receptors for Switchable Multi-Marker Targeting
Separating CAR recognition and signaling polypeptides enables tunable immune activation, marker switching, and logic-based cancer targeting.
- CRISPR Epigenetic Repression of HBV Genes Without DNA Disruption
Targeted dCas9 repressor modules silence HBV DNA transcription to cut viral replication and protein levels without genetic disruption.
- Thioredoxin-Bound DNA Polymerase for Low-Stutter STR Amplification
An engineered DNA polymerase with TBD and thioredoxin cuts STR stutter artifacts without DTT or TCEP, simplifying PCR workflows.
- AAV-Split CRISPR Repressors for In Vivo Gene Silencing
Splitting dCas9 and KRAB across AAV vectors overcomes packaging limits while enabling stable, targeted in vivo gene repression.
- Targeted Epigenetic Activation of 15q11-13 Genes With Non-Viral CRISPR
A locus-specific CRISPR epigenetic system reactivates maternal 15q11-13 genes while limiting off-target effects through non-viral delivery.
- Re-Editable Template Sequences for Precise Multi-Round Genome Editing
A unique re-editable template sequence enables precise repeat genome edits while limiting unwanted cleavage, off-target effects, and cell-line rework.
- APOBEC1 Base Editor Variants for Low Off-Target RNA Editing
Specific APOBEC1 mutations suppress unwanted RNA and DNA edits while preserving Cas9-guided cytidine base editing precision.
- Engineered Transcription Repressors for Durable Allele Silencing
Engineered transcription repressors target regulatory DNA and poly(CAG) tracts to durably silence pathogenic alleles with fewer invasive treatments.
- Htt Repressors With AAV9 Delivery for Mutant Allele Suppression
Engineered zinc finger, TALE, and CRISPR Htt repressors use AAV9 brain delivery to suppress mutant alleles and reduce HD-related neuronal damage.
- Type V dCRISPR Repressors for Specific Gene Silencing
Catalytically dead Type V CRISPR fused to repressor domains enables targeted gene silencing with lower off-target effects and flexible gRNA retargeting.
- HbW Polynucleotide Editing for Break-Free Gene Modification
HbW-based editing agents enable precise gene modification without DNA breaks, reducing off-target effects and working in non-replicating cells.