Fusion With DNA-Binding Domain TRIZ Cases
- Recruitment-Domain Fusion Complex for Efficient Target Gene Repression
Interacting recruitment domains bring methylation and repressor fusions together to improve recruitment efficiency and target gene inhibition.
- Genome Modulation Polypeptide for Precise Long-Sequence Insertion
A single polypeptide combines target recognition, DNA cleavage, and reverse transcription to insert longer sequences at defined genomic sites.
- Adenine Deaminase Fusion Proteins for Eukaryotic Base Editing
Adenine deaminase fusion proteins pair catalytic editing with nucleic acid targeting for precise A-T to G-C conversion in eukaryotic cells.
- Prime Editor Variants Using MMR Inhibition for Precise Editing
MMR inhibition with MLH1dn or nearby silent mutations helps prime editing increase efficiency while reducing indel byproducts.
- Epigenetic Editing Fusion Proteins Without DNA Double-Strand Breaks
Fusion proteins pair DNA-binding domains with methyltransferases to modify gene regulation without double-strand breaks or indels.
- Recruitment-Domain Fusion Complex for Targeted Gene Repression
Interacting recruitment domains address weak recruitment and limited modification range by pairing DNA methylation and repressor fusions for target gene inhibition.
- Targeted CRISPR Gene Repression for HBV Replication Control
CRISPR-Cas fusion proteins target multiple HBV DNA sites to repress viral transcription and reduce replication and protein levels.
- Chimera DNA Polymerase Domains for High-Fidelity PCR
Combining Pfu, KOD, T4, and phi29 domains balances proofreading fidelity, processivity, amplification speed, and inhibitor tolerance in PCR.
- CRISPR-Cas Delta Endonuclease for Compact Gene Editing
A compact Casδ endonuclease addresses large CRISPR proteins and multi-RNA guidance through a truncated catalytic core for precise editing.
- PPR ssDNA-Binding Domains for Programmable Gene Regulation
Modular PPR repeats recognize target ssDNA sequences, enabling programmable gene-expression regulation beyond natural ssDNA-binding proteins.