Fusion With DNA-Binding Domain TRIZ Cases
- Altered PAM Specificity SpCas9 Variants for Expanded Genome Targeting
Mutated SpCas9 nucleases recognize novel PAM sequences, resolving the trade-off between targeting range and sequence specificity.
- Cytosine Base Editing System Loop Mutations
Loop mutations in the hA3Bctd domain tune deaminase binding strength to narrow the editing window and reduce genome-wide off-target effects.
- Targeted Genomic Alteration via Multiple Insertion Sites
Multiple insertion sites enable targeted integration of exogenous nucleic acid sequences into predetermined genomic locations.
- Peptide Construct for Chromatin Targeting and DNA Break Induction
A nucleotide construct encodes a peptide with a targeting domain that binds to hypomethylated chromatin in cancer cells.
- Mutant TcBuster Transposase Charge Engineering for Gene Transfer
Increasing net charge via amino acid substitutions boosts transposition efficiency, resolving low delivery rates into immune system cells.
- Engineered Zinc Finger Proteins for EPSPS Gene Targeting
Zinc finger nucleases target paralogous EPSPS genes with high precision, resolving low efficiency in conventional plant genome editing.
- Repeatable Directed Endonucleases for Offset DNA Cleavage
Repeatable directed endonucleases cleave DNA at an offset position to preserve the recognition sequence, enabling precise genome engineering and data recording.