Fusion With DNA-Binding Domain TRIZ Cases
- RNA-Guided Endonuclease Targeting Precision via Guide RNA Segmentation
Replacing custom protein design with a universal nuclease and programmable RNA eliminates costly target-specific engineering while reducing off-target effects.
- Polypeptide DNA-Binding Specificity via Periodic Residue Variation
Periodic variation in every four modules reduces non-specific binding while maintaining high cleavage activity and simplifying vector preparation.
- Zinc Finger Nuclease Homologous Recombination in Plant Cells
Engineered zinc finger nucleases create double-strand breaks to increase homologous recombination frequency, overcoming rare meganuclease site limitations.
- Light-Activated Protein Phosphatase Operon for Spatiotemporal Control
Segmented genetic operons combine light-sensitive and catalytic domains to achieve spatiotemporal control over protein phosphatase activity.
- Methylcytosine-Selective Deaminases for Epigenetic Sequencing
A methylcytosine-selective deaminase converts 5mC and 5hmC to thymidine while leaving cytosine unaltered.
- Anti-IL-6 Agent for Post-Surgical Pain Prevention
Prophylactic anti-IL-6 agents block interleukin 6 signaling to prevent chronic post-operative pain persistence.
- Artificial Transcription Repressors for Permanent Epigenetic Gene Silencing
Combinatorial artificial transcription repressors fuse DNA-binding domains with epigenetic effectors to establish heritable gene silencing.
- Chimeric Polymerase SSB Fusion for DNA Replication Processivity
A chimeric protein fuses a single-stranded nucleic-acid-binding domain with a catalytic polymerase to increase replication speed.
- PCR Mixtures with Arginine and Sso7 for Specificity
Adding arginine and Sso7 conjugates to PCR mixtures suppresses non-specific product formation while increasing tolerance to inhibitors.
- Sso7 Polymerase Conjugate Mutations Reduce Non-Specific Amplification
Targeted mutations at positions 28, 43, and 53 in the Sso7 domain of polymerase conjugates reduce non-specific amplification while maintaining processivity.
- Chimeric Polypeptides for Site-Specific Genome Recombination
Chimeric polypeptides fuse TALE DNA-binding domains with nuclease modules to enable precise site-specific genome recombination.