Fusion With DNA-Binding Domain TRIZ Cases
- Fusion RT variants for RNA structure interference
Engineered fusion reverse transcriptases with specific mutations enhance transcription efficiency by resolving RNA secondary structure interference.
- Single Base Editing HBA2 Gene Repair
A single base editor converts mutated cytosine to thymine in the HBA2 gene sequence without inducing double-strand breaks.
- Safe Harbor Locus Transgene Integration for Malignancy Risk Reduction
Targeted integration into albumin gene loci eliminates malignancy risks from random insertion while sustaining durable expression.
- Hybrid Polymerase for Long Amplicon Amplification
A hybrid polymerase conjugate enhances nucleic acid amplification efficiency through linked binding domains.
- TALE-Nuclease Design via Alternative RVD Matrix
An alternative RVD matrix maps repeat variable diresidues to nucleic acid bases, streamlining TALE-nuclease assembly and reducing off-target events.
- Engineered TALE Polypeptides for Precise Genome Targeting
Segmented TALE monomers enable precise genome targeting, resolving the trade-off between manipulation efficiency and binding specificity.
- Bidirectional Constitutive Promoter for Stable Transgene Co-expression
A bidirectional constitutive promoter drives simultaneous expression of multiple transgenes in plant cells using a single regulatory element.
- Thermophilic DNA Polymerase Mutants for Inhibitor Resistance
Mutant thermophilic DNA polymerases with neutral amino acid residues at position 379 enable efficient nucleic acid amplification.
- Platinum TALEN Editing for TCR Gene Replacement
Modified TALENs delete endogenous T cell receptors in regulatory T cells to prevent co-expression and ensure high specificity.
- Recombinant Fusion Protein for Efficient Genomic Editing
A recombinant fusion protein recruits linear DNA inserts to genomic cleavage sites.
- Yeast Three-Hybrid Assay for High-Throughput Metabolic Engineering
Genetically engineered yeast cells express fusion proteins to detect therapeutic molecules via chemical inducers of dimerization.
- Modular Metabolite Biosensor Design via Protein Segmentation
Modular fusion proteins detect metabolites to overcome limited biosensor availability.
- Gene-Regulating Compositions for Immune Cell Exhaustion
Reducing endogenous target genes in immune cells using CRISPR-Cas or siRNA to overcome exhaustion in solid malignancies.
- Cas12a Nuclease Editing 4qA Signal for Muscular Dystrophy
Cas12a nuclease proteins disrupt the 4qA polyadenylation signal sequence to reduce DUX4 expression, addressing permanent genetic mutation repair.
- TALEN Cleavage of Latent HSV-1 Genomes
TALENs cleave latent HSV-1 genomes via AAV delivery, eliminating persistent viral loads that conventional antivirals cannot address.
- De Novo Designed DNA Binding Proteins for Therapeutic Delivery
De novo designed small helical polypeptides achieve programmable DNA targeting while bypassing the size and delivery limits of CRISPR-Cas systems.
- Coiled Coil Reporter for Protein Interaction Imaging
Phase-shifted droplets concentrate fluorescent proteins to overcome tissue autofluorescence and light scattering during imaging.
- Multivalent Transcription Factor Assembly for Gene Expression Control
A modular molecular complex tethers synthetic transcription factors to tandem DNA binding motifs via a molecular clamp.
- Zinc Finger Fusion Proteins Repress Alpha-Synuclein
Zinc finger fusion proteins repress alpha-synuclein expression via targeted SNCA gene binding.