Hydrolases TRIZ Cases

Cas9 RNPs and donor DNA templates enable targeted insertion of sequences over 200 nucleotides while reducing off-target effects and cell viability loss.

See how long ssDNA templates with short homology arms improve large-sequence insertion through CRISPR/Cas and homology-directed repair.

Genetically installed surface affinity tags and intracellular fluorescent reporters support FACS or MACS sorting with improved cell purity.

Surfactants inhibit conventional lipases in cleaners; targeted sequence changes preserve triglyceride hydrolysis and improve detergency.

Thermostable Cas variants preserve collateral cleavage above 60–65°C, enabling reliable nucleic acid amplification and detection in one vessel.

Cationic polymers bind nucleic acids into complexes that support CRISPR delivery while reducing viral-vector manufacturing and safety constraints.

Short DNA fragments are difficult to assemble with complex enzyme mixes; purified XthA exonuclease enables seamless joining down to 70 bp.

Mutations in DCL3, 4CL06, and RLK2 help solanaceous plants resist Begomovirus infection, propagation, and symptom expression.

Labor-intensive mammalian gene tagging can disrupt protein function; HITAG uses orthogonal Cas nucleases and NHEJ for scalable C-terminal insertion.

Specific SpCas9 mutations such as D54A improve on-target DNA cleavage in human cells while maintaining editing specificity.

Targeted substitutions at six protease residues improve PLA-degrading activity and stability at temperatures up to 80°C.

Specific amino acid substitutions help protease variants retain activity and half-life while degrading polylactic acid at 40–90°C.

Single-site PHB depolymerase mutations support stable, active PHA degradation at 45°C or above in industrial treatment.

Arabinose promoters, codon-optimized genes, and His-tag purification enable in-house enzymes for higher-quality mRNA and cell transfection.

CRISPR-Cas9 editing of HTR2A or SLAMF8 creates an immortalized avian platform for high-titer MDV growth without primary CEF preparation.

CRISPR/Cas9 disruption of PARP2 produces smaller plants with faster fruiting and less inedible biomass for confined farms.

A temporary selection marker identifies HDR-edited cells first, then a second HDR step removes it without leaving genetic remnants.

Replace RNAi knockdown screens with programmable CRISPR-Cas guide RNAs for scalable, precise gene knockouts and reduced off-target activity.

Engineered Vibrio natriegens cells retain plasmids without antibiotic selection while increasing growth and yields and reducing endotoxin secretion.

Removing antibiotic resistance genes supports stable target protein production while reducing product contamination and environmental leakage.

A native BnIND-A deletion helps Brassica plants resist pod shatter, reducing seed loss and preserving agronomic suitability.