Hydrolases TRIZ Cases

Ultrastable enzymes digest biopolymers at high temperature and low pH, preserving samples for higher-quality mass spectrometric data.

This case uses muscle-specific promoters in AAV vectors to restore calpain 3 activity in skeletal muscle with minimal cardiac impact.

This case combines inner-ear AAV delivery of TMPRSS3 or LOXHD1 with cochlear implants to preserve spiral ganglion cells.

TGF-β inhibition, Shh activation, and PSEN2 repair generate reproducible BFCNs with restored excitability.

Ex vivo loading of nucleated cells with mutant Ras antigens helps induce potent T cell responses against Ras-mutant cancers.

A sequence-modified aminoacylase hydrolyzes the difficult ortho isomer, enabling total hippuric acid measurement through glycine.

This case places promoter or protein-expression cargo into bacterial chromosomes to reduce plasmid variation and experimental noise.

Chemical catalyst removal and excess methanol are addressed with dual sn-1,3 and sn-2 lipases plus controlled alcohol addition.

Predefined DNA tags, suppression PCR, and rhAmpSeq improve sensitivity and accuracy when nominating CRISPR on- and off-target sites.

This case links circulating MZF1, AMH, TIMP1, and GNMT levels to risk stratification and targeted inhibitor treatment.

Cas endonuclease and guide RNA target fungal DNA while NHEJ suppression improves donor-DNA homologous recombination.

Smaller Staphylococcus-derived sRGNs improve eukaryotic editing activity, fidelity, and viral-vector compatibility.

This case uses modular guide RNA and Cas cassettes to enable targeted fungal genome edits without requiring homologous recombination.

Targeted Cas9 mutations alter PAM recognition, enabling cleavage to respond differently to methylated cytosine while retaining activity.

Dual selection with DPH-sensitive toxins and HRM PCR separates zygosity and integration events for CRISPR analysis.

This case uses reporter cleavage rates and KM or k*cat/KM to improve SNP differentiation when target concentration is unknown.

RNA-programmable CRISPR vectors simplify multi-site genome targeting, with nuclear localization sequences supporting nuclear accumulation.

This case uses guide RNA and engineered CRISPR functional domains to simplify precise genome editing and improve scalability.

This case uses Tat-responsive promoter elements to target latent HIV DNA while limiting constitutive CRISPR exposure.

Reporter-linked vectors enable rapid screening of multiple functional molecules in cells.

This case uses ligand-binding inducible caspase polypeptides to selectively eliminate modified cells while minimizing host immune response.

Targeted editing of SMO1-L, DWF7-L, and TAM iso 2 lowers SGAs while preserving plant growth and supporting protein and fiber recovery.

Fluorescent markers and flow cytometry identify uniform Cas expression, improving targeted mutation and donor DNA incorporation.

Sc+ and Sc++ modify the PAM-interacting region to recognize NG and NNG targets while retaining high-fidelity editing.

Targeted trehalose, RNA-binding, and SGI1 gene attenuation can raise algal lipid and biomass productivity for biofuel use.

This case uses endocytosis, plasma treatment, and selection to produce transformed cells with reduced cytotoxicity and no retained vector.

A self-removing CRISPR cassette excises itself after editing, reducing metabolic burden and enabling further plant cell transformations.

Modular guide RNAs and engineered CRISPR effectors expand target-site versatility while supporting precise nucleic acid modification.

Engineered binding domains and barcodes profile DNA and RNA modifications at single-base resolution.

Guide RNAs and ex vivo CRISPR editing target HBG1/HBG2 or BCL11A to raise fetal hemoglobin while using autologous cells.

Endonuclease and Cas-based enrichment separates strands and uses molecular barcodes to reduce PCR artifacts and detect rare variants.

This case uses targeted SIGRXC9 knockout to improve tomato chilling tolerance while managing dwarf growth and yield tradeoffs.

Cytolysin-mediated host cell lysis followed by DNase digestion reduces host DNA interference for broad pathogen sequencing.

A lipase route uses a structured intermediate to raise POS content in crude product.

This case uses evolved Bxb1 and PhiC31 integrases to improve large-cargo genome insertion while limiting off-target effects.

Heterologous polynucleotides and Cas-guided breaks improve homology-directed repair in plant genomes.

CRISPR disruption of MHC-I and MHC-II genes with PD-L1 or HLA-E supports immune evasion and cell survival after engraftment.

This case uses intracellular phosphatase and synthase pathways to produce sialylated compounds with less energy and fewer byproducts.

This case uses optimized nuclease sequences, guide RNAs, and fusion domains to improve precision and efficiency in eukaryotic cells.

Dual guide RNAs and retron ncRNA synthesize donor DNA in situ, enabling precise large genomic insertions, deletions, and replacements.

Genome-wide screening identifies regulators for CRISPR/Cas9 editing, helping CAR- and TCR-T cells resist exhaustion and immunosuppression.

This case uses Spinacia tetrandra genomic fragments and gene stacking to protect spinach against Peronospora and other pathogens.

Target polynucleotides move stepwise through a nanopore as modified Dda helicases enable rapid electrical sequencing without amplification.

This case combines DNA-PK and Polθ inhibitors to suppress NHEJ and MMEJ, reducing unwanted insertions or deletions during HDR editing.

PEgRNA-guided prime editing corrects NCF1 mutations through single-strand nicking, avoiding rearrangements near NCF1B and NCF1C.

CRISPR deletion of LNC000093 guides iPSCs through rosettes and neurospheres toward RGCs, with single-cell RNA sequencing for monitoring.

This case shows how PAD converts arginine to citrulline, reducing trypsin inhibitor activity and improving protein taste.

Targeted CD163 exon 7 inactivation improves PRRSv resistance while guide RNA design helps limit unintended genomic edits.

One-sided transposition preserves DNA contiguity and haplotype data.

Genetically engineered C1-fixing microbes sustain tandem-repeat protein and chemical production from CO, CO2, and H2.